Rw2G010200
NAC Family

NAC domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0005584
Physical Location & Seq
Reverse (-)
10308160 .. 10312698
4539 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G010200.1

Sequence Viewer

Length: 1089 bp
ATGGAGTCAATCGAGTCCACTGTTCCGCCAGGTTTTCGATTTCATCCGACTGATGAAGAACTCGTTGGTTATTACCTTAGAAAGAAAGTTGCATCGCAAAAGATCGATCTCGATGTCATCAGAGACATTGATCTCTACCGAATAGAACCATGGGATCTGCAAGATAGATGCCGGATCGGGTATGAGGAGCAGAATGAATGGTATTTCTTCAGCCACAAAGATAAGAAGTATCCGACGGGCACAAGGACCAACAGGGCTACCATGGCCGGGTTTTGGAAAGCGACGGGAAGGGACAAATCGGTGTACGATAAGTCCAAGCTGATAGGAATGAGGAAAACTCTTGTTTTCTACAAAGGGAGAGCTCCGAATGGACAGAAAACCGACTGGATCATGCACGAATACCGACTCGAATCCGACGAAAATGGACCACCACAGGAAGAAGGATGGGTGGTATGCCGAGCATTCAAGAAGAGAACCAGTAGCCAAAACAAAAGCATGGAAGGGTGGGACTCAAGCTACTTCTACGACGAACTTCACGGTGTCAGCTCCATCGTGGATCCAGCCGATTTCATCTCGAGGCATCCCCAAAGGGCGGCGCAAAGCTTCTTAGCTGCCCAGAGTTTCTTGTGTAAGCAGGAGATCACAGCTGAAGCCGACAATTTGAATAGTACTTTCATGCCATCTGATCATTTTGTCCAGCTCCCTCAGCTAGAGAGCCCATCTCTTCCCTTAATAAAGAGGCCGAACTCATCAATGTCTCTGATATCTTCGTCGGAGAATAATGTCGAAGATGACGTGGAGTTGATTAATAATAGAGGGTGCAGCAGTAACATTATTAATGCCAATAAAGCAGCTGCAGTGACTGACTGGAGGGACCTGGACAAGTTTGTAGCTTCTCAGTTGAGTCAAGAAGATAAGTATAATGAATTAGGTGATCATGGAGAGTACTCAAGCTTTGGTGTTCATGATCATGATGATTCAGATATGGCGGCATTGTTGTTGTTGCAGAGGGATGATGGGAACAAGTTGAATGGATTTTTAAGTTCGAGCTCGGATTGTGATCTGGGAATATGCATATTTGAAAAATGA

Protein Analysis

362

Amino Acids

41.35

Weight (kDa)

5.11

Isoelectric Point (pI)

49.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NAM PF02365 9 - 136 7.7e-49 No apical meristem (NAM) protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 26, 593, 989
AclWI GGATC 5 cut(s) 162, 182, 395, 551, 564
AcoI YGGCCR 1 cut(s) 264
AcuI CTGAAG 2 cut(s) 193, 669
AfaI GTAC 3 cut(s) 305, 670, 947
AfiI CCNNNNNNNGG 3 cut(s) 267, 273, 592
AgsI TTSAA 4 cut(s) 466, 664, 1030, 1082
AjiI CACGTC 1 cut(s) 796
AjnI CCWGG 2 cut(s) 28, 876
AjuI GAANNNNNNNTTGG 2 cut(s) 48, 80
Alw21I GWGCWC 2 cut(s) 364, 1052
Alw26I GTCTC 2 cut(s) 117, 762
AlwI GGATC 5 cut(s) 162, 182, 395, 551, 564
AlwNI CAGNNNCTG 1 cut(s) 863
Ama87I CYCGRG 1 cut(s) 574
AoxI GGCC 2 cut(s) 264, 740
ApeKI GCWGC 4 cut(s) 611, 822, 851, 854
AseI ATTAAT 2 cut(s) 807, 837
AspLEI GCGC 1 cut(s) 598
AspS9I GGNCC 3 cut(s) 246, 425, 874
AsuC2I CCSGG 1 cut(s) 268
AsuHPI GGTGA 1 cut(s) 944
AvaI CYCGRG 1 cut(s) 574
AvaII GGWCC 3 cut(s) 246, 425, 874
BaeGI GKGCMC 1 cut(s) 242
BamHI GGATCC 1 cut(s) 556
BanII GRGCYC 3 cut(s) 364, 719, 1052
Bbv12I GWGCWC 2 cut(s) 364, 1052
BbvCI CCTCAGC 1 cut(s) 705
BbvI GCAGC 4 cut(s) 598, 834, 841, 863
BccI CCATC 5 cut(s) 438, 557, 688, 727, 1010
BciT130I CCWGG 2 cut(s) 30, 878
BciVI GTATCC 1 cut(s) 240
BclI TGATCA 3 cut(s) 685, 934, 967
BcnI CCSGG 1 cut(s) 268
BcoDI GTCTC 2 cut(s) 117, 762
BfaI CTAG 1 cut(s) 710
BfmI CTRYAG 1 cut(s) 855
BfuI GTATCC 1 cut(s) 240
BisI GCNGC 6 cut(s) 594, 612, 823, 852, 855, 990
BlsI GCNGC 6 cut(s) 595, 613, 824, 853, 856, 991
BmcAI AGTACT 2 cut(s) 670, 947
Bme1390I CCNGG 3 cut(s) 30, 268, 878
Bme18I GGWCC 3 cut(s) 246, 425, 874
BmeT110I CYCGRG 1 cut(s) 574
BmgBI CACGTC 1 cut(s) 796
BmgT120I GGNCC 3 cut(s) 246, 425, 874
BmiI GGNNCC 2 cut(s) 558, 875
BmrFI CCNGG 3 cut(s) 30, 268, 878
BmsI GCATC 3 cut(s) 101, 158, 589
BplI GAGNNNNNCTC 4 cut(s) 322, 354, 706, 738
BpmI CTGGAG 1 cut(s) 889
Bpu10I CCTNAGC 1 cut(s) 705
BpuEI CTTGAG 2 cut(s) 496, 934
BpuMI CCSGG 1 cut(s) 268
Bsa29I ATCGAT 1 cut(s) 105
BsaBI GATNNNNATC 1 cut(s) 1059
BsaJI CCNNGG 2 cut(s) 149, 261
Bsc4I CCNNNNNNNGG 3 cut(s) 267, 273, 592
Bse1I ACTGG 3 cut(s) 389, 477, 872
Bse8I GATNNNNATC 1 cut(s) 1059
BseBI CCWGG 2 cut(s) 30, 878
BseCI ATCGAT 1 cut(s) 105
BseDI CCNNGG 2 cut(s) 149, 261
BseGI GGATG 4 cut(s) 43, 449, 580, 1018
BseJI GATNNNNATC 1 cut(s) 1059
BseLI CCNNNNNNNGG 3 cut(s) 267, 273, 592
BseMII CTCAG 2 cut(s) 719, 911
BseNI ACTGG 3 cut(s) 389, 477, 872
BseRI GAGGAG 1 cut(s) 200
BseSI GKGCMC 1 cut(s) 242
BseXI GCAGC 4 cut(s) 598, 834, 841, 863
BsgI GTGCAG 1 cut(s) 841
BshFI GGCC 2 cut(s) 266, 742
BshVI ATCGAT 1 cut(s) 105
BsiHKAI GWGCWC 2 cut(s) 364, 1052
BsiHKCI CYCGRG 1 cut(s) 574
BsiSI CCGG 2 cut(s) 172, 267
BslFI GGGAC 3 cut(s) 305, 521, 887
BslI CCNNNNNNNGG 3 cut(s) 267, 273, 592
BsmAI GTCTC 2 cut(s) 117, 762
BsmFI GGGAC 3 cut(s) 305, 521, 887
BsmI GAATGC 1 cut(s) 461
BsnI GGCC 2 cut(s) 266, 742
BsoBI CYCGRG 1 cut(s) 574
Bsp1286I GDGCHC 4 cut(s) 242, 364, 719, 1052
Bsp19I CCATGG 2 cut(s) 149, 261
BspACI CCGC 3 cut(s) 26, 593, 989
BspANI GGCC 2 cut(s) 266, 742
BspCNI CTCAG 2 cut(s) 718, 910
BspDI ATCGAT 1 cut(s) 105
BspHI TCATGA 2 cut(s) 964, 970
BspLI GGNNCC 2 cut(s) 558, 875
BspMAI CTGCAG 1 cut(s) 859
BspPI GGATC 5 cut(s) 162, 182, 395, 551, 564
BsrI ACTGG 3 cut(s) 389, 477, 872
BssECI CCNNGG 2 cut(s) 149, 261
BssT1I CCWWGG 2 cut(s) 149, 261
Bst2UI CCWGG 2 cut(s) 30, 878
Bst4CI ACNGT 2 cut(s) 22, 539
Bst6I CTCTTC 2 cut(s) 464, 729
BstDEI CTNAG 4 cut(s) 77, 607, 705, 897
BstDSI CCRYGG 2 cut(s) 149, 261
BstF5I GGATG 4 cut(s) 43, 449, 580, 1018
BstHHI GCGC 1 cut(s) 598
BstMAI GTCTC 2 cut(s) 117, 762
BstMWI GCNNNNNNNGC 3 cut(s) 263, 706, 848
BstNI CCWGG 2 cut(s) 30, 878
BstSCI CCNGG 3 cut(s) 28, 266, 876
BstSFI CTRYAG 1 cut(s) 855
BstSLI GKGCMC 1 cut(s) 242
BstV1I GCAGC 4 cut(s) 598, 834, 841, 863
BstX2I RGATCY 2 cut(s) 154, 556
BstYI RGATCY 2 cut(s) 154, 556
Bsu15I ATCGAT 1 cut(s) 105
BsuI GTATCC 1 cut(s) 240
BsuRI GGCC 2 cut(s) 266, 742
BsuTUI ATCGAT 1 cut(s) 105
BtgI CCRYGG 2 cut(s) 149, 261
BtgZI GCGATG 1 cut(s) 78
BtrI CACGTC 1 cut(s) 796
BtsCI GGATG 4 cut(s) 43, 449, 580, 1018
BtsI GCAGTG 1 cut(s) 864
BtsIMutI CAGTG 2 cut(s) 18, 864
CaiI CAGNNNCTG 1 cut(s) 863
CciI TCATGA 2 cut(s) 964, 970
CfoI GCGC 1 cut(s) 598
Cfr13I GGNCC 3 cut(s) 246, 425, 874
ClaI ATCGAT 1 cut(s) 105
Csp6I GTAC 3 cut(s) 304, 669, 946
CviAII CATG 8 cut(s) 150, 262, 391, 496, 676, 938, 965, 971
CviQI GTAC 3 cut(s) 304, 669, 946
DdeI CTNAG 4 cut(s) 77, 607, 705, 897
EaeI YGGCCR 1 cut(s) 264
Eam1104I CTCTTC 2 cut(s) 464, 729
EarI CTCTTC 2 cut(s) 464, 729
EciI GGCGGA 1 cut(s) 15
Ecl136II GAGCTC 2 cut(s) 362, 1050
Eco130I CCWWGG 2 cut(s) 149, 261
Eco24I GRGCYC 3 cut(s) 364, 719, 1052
Eco32I GATATC 1 cut(s) 765
Eco47I GGWCC 3 cut(s) 246, 425, 874
Eco53kI GAGCTC 2 cut(s) 362, 1050
Eco57I CTGAAG 2 cut(s) 193, 669
Eco88I CYCGRG 1 cut(s) 574
EcoICRI GAGCTC 2 cut(s) 362, 1050
EcoO109I RGGNCCY 1 cut(s) 874
EcoRII CCWGG 2 cut(s) 28, 876
EcoRV GATATC 1 cut(s) 765
EcoT14I CCWWGG 2 cut(s) 149, 261
EcoT22I ATGCAT 1 cut(s) 1076
EcoT38I GRGCYC 3 cut(s) 364, 719, 1052
ErhI CCWWGG 2 cut(s) 149, 261
FaeI CATG 8 cut(s) 153, 265, 394, 499, 679, 941, 968, 974
FaqI GGGAC 3 cut(s) 305, 521, 887
FatI CATG 8 cut(s) 149, 261, 390, 495, 675, 937, 964, 970
FbaI TGATCA 3 cut(s) 685, 934, 967
Fnu4HI GCNGC 6 cut(s) 594, 612, 823, 852, 855, 990
FokI GGATG 4 cut(s) 30, 456, 567, 1025
FriOI GRGCYC 3 cut(s) 364, 719, 1052
Fsp4HI GCNGC 6 cut(s) 594, 612, 823, 852, 855, 990
FspBI CTAG 1 cut(s) 710
GlaI GCGC 1 cut(s) 597
GluI GCNGC 6 cut(s) 594, 612, 823, 852, 855, 990
GsuI CTGGAG 1 cut(s) 889
HaeIII GGCC 2 cut(s) 266, 742
HapII CCGG 2 cut(s) 172, 267
HhaI GCGC 1 cut(s) 598
Hin1II CATG 8 cut(s) 153, 265, 394, 499, 679, 941, 968, 974
Hin6I GCGC 1 cut(s) 596
HinP1I GCGC 1 cut(s) 596
HindIII AAGCTT 2 cut(s) 601, 952
HinfI GANTC 7 cut(s) 5, 14, 405, 410, 509, 904, 977
HpaII CCGG 2 cut(s) 172, 267
HphI GGTGA 1 cut(s) 944
Hpy166II GTNNAC 2 cut(s) 18, 304
Hpy188III TCNNGA 6 cut(s) 110, 466, 574, 908, 965, 971
Hpy8I GTNNAC 2 cut(s) 18, 304
Hpy99I CGWCG 5 cut(s) 238, 286, 419, 530, 775
HpyAV CCTTC 3 cut(s) 282, 434, 494
HpyCH4III ACNGT 2 cut(s) 22, 539
HpyCH4IV ACGT 1 cut(s) 795
HpyCH4V TGCA 7 cut(s) 92, 160, 394, 822, 857, 1006, 1074
HpyF10VI GCNNNNNNNGC 3 cut(s) 263, 706, 848
HpyF3I CTNAG 4 cut(s) 77, 607, 705, 897
HpySE526I ACGT 1 cut(s) 795
Hsp92II CATG 8 cut(s) 153, 265, 394, 499, 679, 941, 968, 974
HspAI GCGC 1 cut(s) 596
Ksp22I TGATCA 3 cut(s) 685, 934, 967
LmnI GCTCC 4 cut(s) 187, 367, 551, 705
Lsp1109I GCAGC 4 cut(s) 598, 834, 841, 863
LweI GCATC 3 cut(s) 101, 158, 589
MaeI CTAG 1 cut(s) 710
MaeII ACGT 1 cut(s) 795
MaeIII GTNAC 2 cut(s) 827, 859
MboII GAAGA 8 cut(s) 68, 199, 449, 481, 716, 759, 800, 923
MflI RGATCY 2 cut(s) 154, 556
MhlI GDGCHC 4 cut(s) 242, 364, 719, 1052
MluCI AATT 2 cut(s) 658, 926
MlyI GAGTC 5 cut(s) 14, 23, 399, 503, 913
MmeI TCCRAC 4 cut(s) 71, 257, 438, 753
MnlI CCTC 8 cut(s) 178, 324, 570, 714, 732, 809, 864, 1002
Mph1103I ATGCAT 1 cut(s) 1076
MseI TTAA 4 cut(s) 731, 807, 837, 1040
MslI CAYNNNNRTG 1 cut(s) 969
MspA1I CMGCKG 2 cut(s) 647, 854
MspI CCGG 2 cut(s) 172, 267
MspR9I CCNGG 3 cut(s) 30, 268, 878
Mva1269I GAATGC 1 cut(s) 461
MvaI CCWGG 2 cut(s) 30, 878
MwoI GCNNNNNNNGC 3 cut(s) 263, 706, 848
NciI CCSGG 1 cut(s) 268
NcoI CCATGG 2 cut(s) 149, 261
NlaIII CATG 8 cut(s) 153, 265, 394, 499, 679, 941, 968, 974
NlaIV GGNNCC 2 cut(s) 558, 875
NmeAIII GCCGAG 1 cut(s) 482
NmuCI GTSAC 1 cut(s) 859
NsiI ATGCAT 1 cut(s) 1076
PaeR7I CTCGAG 1 cut(s) 574
PagI TCATGA 2 cut(s) 964, 970
PcsI WCGNNNNNNNCGW 2 cut(s) 414, 792
PctI GAATGC 1 cut(s) 461
PfeI GAWTC 2 cut(s) 410, 977
PkrI GCNGC 6 cut(s) 595, 613, 824, 853, 856, 991
PleI GAGTC 5 cut(s) 13, 22, 399, 503, 912
PpsI GAGTC 5 cut(s) 13, 22, 399, 503, 912
PpuMI RGGWCCY 1 cut(s) 874
PshBI ATTAAT 2 cut(s) 807, 837
Psp124BI GAGCTC 2 cut(s) 364, 1052
Psp5II RGGWCCY 1 cut(s) 874
Psp6I CCWGG 2 cut(s) 28, 876
PspGI CCWGG 2 cut(s) 28, 876
PspN4I GGNNCC 2 cut(s) 558, 875
PspPI GGNCC 3 cut(s) 246, 425, 874
PspPPI RGGWCCY 1 cut(s) 874
PstI CTGCAG 1 cut(s) 859
PstNI CAGNNNCTG 1 cut(s) 863
PsuI RGATCY 2 cut(s) 154, 556
PvuII CAGCTG 2 cut(s) 647, 854
RsaI GTAC 3 cut(s) 305, 670, 947
RsaNI GTAC 3 cut(s) 304, 669, 946
RseI CAYNNNNRTG 1 cut(s) 969
SacI GAGCTC 2 cut(s) 364, 1052
SaqAI TTAA 4 cut(s) 731, 807, 837, 1040
SatI GCNGC 6 cut(s) 594, 612, 823, 852, 855, 990
Sau96I GGNCC 3 cut(s) 246, 425, 874
ScaI AGTACT 2 cut(s) 670, 947
SchI GAGTC 5 cut(s) 14, 23, 399, 503, 913
ScrFI CCNGG 3 cut(s) 30, 268, 878
SduI GDGCHC 4 cut(s) 242, 364, 719, 1052
SfaNI GCATC 3 cut(s) 101, 158, 589
SfcI CTRYAG 1 cut(s) 855
Sfr274I CTCGAG 1 cut(s) 574
SinI GGWCC 3 cut(s) 246, 425, 874
SlaI CTCGAG 1 cut(s) 574
SmiMI CAYNNNNRTG 1 cut(s) 969
SmlI CTYRAG 3 cut(s) 511, 574, 949
SmoI CTYRAG 3 cut(s) 511, 574, 949
Sse9I AATT 2 cut(s) 658, 926
SsiI CCGC 3 cut(s) 26, 593, 989
SspMI CTAG 1 cut(s) 710
SstI GAGCTC 2 cut(s) 364, 1052
StyD4I CCNGG 3 cut(s) 28, 266, 876
StyI CCWWGG 2 cut(s) 149, 261
TaaI ACNGT 2 cut(s) 22, 539
TaiI ACGT 1 cut(s) 798
TaqI TCGA 8 cut(s) 12, 37, 105, 111, 408, 575, 786, 1046
TasI AATT 2 cut(s) 658, 926
TatI WGTACW 2 cut(s) 668, 945
TauI GCSGC 2 cut(s) 596, 992
TfiI GAWTC 2 cut(s) 410, 977
Tru1I TTAA 4 cut(s) 731, 807, 837, 1040
Tru9I TTAA 4 cut(s) 731, 807, 837, 1040
TscAI CASTG 2 cut(s) 25, 864
TseFI GTSAC 1 cut(s) 859
TseI GCWGC 4 cut(s) 611, 822, 851, 854
Tsp45I GTSAC 1 cut(s) 859
TspDTI ATGAA 7 cut(s) 32, 69, 210, 559, 664, 939, 953
TspRI CASTG 2 cut(s) 25, 864
VpaK11BI GGWCC 3 cut(s) 246, 425, 874
VspI ATTAAT 2 cut(s) 807, 837
XhoI CTCGAG 1 cut(s) 574
XspI CTAG 1 cut(s) 710
ZrmI AGTACT 2 cut(s) 670, 947
Zsp2I ATGCAT 1 cut(s) 1076
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.