MD02G1145300.v1.1

Tubby-like F-box protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0010770
Physical Location & Seq
Forward (+)
12013585 .. 12016279
2695 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1145300.v1.1.491

Sequence Viewer

Length: 1209 bp
ATGAAAGATGGAATTGGGAGCATGTCAAGGCGAGTTGGTGAGCGGAAGCTTTGGCATAGAAGGACGTTGTCGCATATTGCTCCTGATATGGCTCCAACCCCTCCAACTGATGTGATTCAGCAGGGTCAGTGGGCAAACTTGCCTCCGGAATTGCTTTTGGACATAATTCAGAGGGTAGAAGAAAGTGAAACCTCGTGGCCTGCTCGAGCTGTCGTTGTTTTTTGTGGTTCAGTTTGTAAGTCATGGAGGGACATTACGGGGGAGATAGTCAAAACTCCTGAGCAATGTGGAAGGCTCACATTTCCAATCTCATTGAAGCAGCCGGGGCCTCGCCAGTCTCCAATACAGTGCTTCATTAGAAGGGATAGAGGAACTTCCACATTTTATCTGTACTATGGTCTGGCACCATCTGAGGGTGAGAAAGATAAATTATTGCTAGCAGCCAAAAGGATCAGAAGGGCAACAAGCACGGAGTTCGTTATATCTTTGGTTTCAGATGATTTTTCTCGAGCCAGCAGTGCATATGTTGGTAAATTGAGGTCCAACTTTTTGGGTACCAAGTTCACCGTATACGACAGCCAGCCTCCATGTGATGCGGTACCCAAACCAAAAAATCGATCATCTCGAAGGTTCCATTCTAAGCAGGTATCTCCAAGAGTACCAGCGTGTAACTACAGCGTTGGCAACATCTCTTACGAGCTCAATATTTTTTGCACAAGAGGTCCAAGGAGAATGCATTGTGCTATGCACTCCATTCCTGTCTCGTCCATTCAGGAGGGGGGCACCGCCCCAACACCAACATCGTTGCTACAAGCCTATGACGAACAATTTGCTGTCTCACCAAGTTCAAAAGAAAAGGATCTGGTCGCAGAGTTCAGCTCTACAACCCTCTCGGAGCCAAACGTGTCAGCCCCGGGTGCAGAGCCATTGGTTTTGAAAAACAAGTCTCCTAGGTGGCATGAGCAGCTACAATGCTGGTGCCTTAACTTTAGAGGACGCGTTACAGTGGCTTCTGTGAAAAACTTCCAGCTTGTGGCAGCTGTTGATCCGTCCCACAACGTTTCGGCTGAAGAGCAAGAGAGGGTAATCCTACAGTTTGGAAAGATTGGAAAAGACATCTTCACCTTGGATTATGGCTACCCGCTGTCCTGTTTCCAAGCGTTTGCGATCTGCTTAAGCAGCTTCGACACCAAGCCAGCGTGTGAATGA

Protein Analysis

403

Amino Acids

44.81

Weight (kDa)

9.05

Isoelectric Point (pI)

66.29

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Tub PF01167 105 - 397 5.9e-102 Tub family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0010770)

Species Orthologous Gene IDs
arabidopsis_thaliana AT2G18280 AT2G18280
fragaria_vesca FvH4_1g13070
malus_domestica MD02G1145300.v1.1 MD14G1114200.v1.1 MD15G1259500.v1.1
prunus_persica Prupe.7G156400_v2.0.a1
pyrus_communis pycom02g11500 pycom15g22700
rosa_chinensis RchiOBHm_Chr2g0101241
rosa_laevigata RLG00000017064
rosa_multiflora Rmu_sc0006314.1_g000016
rosa_roxburghii Rroxscaffold_2G00141470
rosa_rugosa Rorug02G0099900 Rorug02G0100000
rosa_samantha Rh2AG145400 Rh2BG150800 Rh2CG151200 Rh2DG150900
rosa_wichuraiana Rw2G011340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 634
Acc65I GGTACC 2 cut(s) 554, 598
AccB1I GGYRCC 5 cut(s) 403, 554, 598, 782, 978
AccB7I CCANNNNNTGG 1 cut(s) 1033
AccBSI CCGCTC 1 cut(s) 43
AccI GTMKAC 1 cut(s) 570
AccII CGCG 1 cut(s) 999
AccIII TCCGGA 1 cut(s) 145
AciI CCGC 4 cut(s) 43, 596, 786, 1142
AclI AACGTT 1 cut(s) 1059
AclWI GGATC 3 cut(s) 458, 867, 1040
AcuI CTGAAG 1 cut(s) 1089
AfaI GTAC 4 cut(s) 392, 556, 600, 660
AfiI CCNNNNNNNGG 2 cut(s) 413, 1033
AflII CTTAAG 1 cut(s) 1174
AflIII ACRYGT 2 cut(s) 903, 997
AgsI TTSAA 3 cut(s) 316, 849, 937
AluBI AGCT 8 cut(s) 49, 209, 700, 879, 967, 1030, 1040, 1182
AluI AGCT 8 cut(s) 49, 209, 700, 879, 967, 1030, 1040, 1182
Alw21I GWGCWC 1 cut(s) 702
Alw26I GTCTC 4 cut(s) 342, 766, 841, 951
AlwI GGATC 3 cut(s) 458, 867, 1040
Ama87I CYCGRG 3 cut(s) 204, 507, 913
Aor13HI TCCGGA 1 cut(s) 145
AoxI GGCC 2 cut(s) 197, 326
ApeKI GCWGC 5 cut(s) 319, 440, 964, 1037, 1179
ArsI GACNNNNNNTTYG 2 cut(s) 812, 844
Asp700I GAANNNNTTC 1 cut(s) 1022
Asp718I GGTACC 2 cut(s) 554, 598
AspA2I CCTAGG 1 cut(s) 950
AspS9I GGNCC 3 cut(s) 326, 540, 722
AsuC2I CCSGG 3 cut(s) 324, 914, 915
AsuHPI GGTGA 5 cut(s) 50, 428, 556, 831, 1114
AsuNHI GCTAGC 1 cut(s) 436
AvaI CYCGRG 3 cut(s) 204, 507, 913
AvaII GGWCC 2 cut(s) 540, 722
AvrII CCTAGG 1 cut(s) 950
BaeGI GKGCMC 1 cut(s) 785
BanI GGYRCC 5 cut(s) 403, 554, 598, 782, 978
BanII GRGCYC 1 cut(s) 702
BauI CACGAG 1 cut(s) 193
Bbv12I GWGCWC 1 cut(s) 702
BbvI GCAGC 5 cut(s) 331, 452, 976, 1049, 1191
BccI CCATC 2 cut(s) 2, 415
BcgI CGANNNNNNTGC 4 cut(s) 457, 491, 812, 846
BcnI CCSGG 3 cut(s) 324, 914, 915
BcoDI GTCTC 4 cut(s) 342, 766, 841, 951
BfaI CTAG 2 cut(s) 437, 951
BfmI CTRYAG 2 cut(s) 673, 1091
BfrI CTTAAG 1 cut(s) 1174
BfuAI ACCTGC 1 cut(s) 634
BisI GCNGC 5 cut(s) 320, 441, 965, 1038, 1180
BlnI CCTAGG 1 cut(s) 950
BlsI GCNGC 5 cut(s) 321, 442, 966, 1039, 1181
Bme1390I CCNGG 3 cut(s) 324, 914, 915
Bme18I GGWCC 2 cut(s) 540, 722
BmeT110I CYCGRG 3 cut(s) 204, 507, 913
BmgT120I GGNCC 3 cut(s) 326, 540, 722
BmiI GGNNCC 9 cut(s) 93, 327, 405, 556, 600, 632, 784, 897, 980
BmrFI CCNGG 3 cut(s) 324, 914, 915
BmsI GCATC 1 cut(s) 583
BmtI GCTAGC 1 cut(s) 440
BplI GAGNNNNNCTC 2 cut(s) 863, 895
Bpu10I CCTNAGC 1 cut(s) 279
BpuMI CCSGG 3 cut(s) 324, 914, 915
Bsa29I ATCGAT 1 cut(s) 616
BsaJI CCNNGG 6 cut(s) 323, 725, 912, 913, 950, 1125
BsaWI WCCGGW 1 cut(s) 145
Bsc4I CCNNNNNNNGG 2 cut(s) 413, 1033
Bse1I ACTGG 1 cut(s) 334
Bse3DI GCAATG 1 cut(s) 290
BseAI TCCGGA 1 cut(s) 145
BseCI ATCGAT 1 cut(s) 616
BseDI CCNNGG 6 cut(s) 323, 725, 912, 913, 950, 1125
BseLI CCNNNNNNNGG 2 cut(s) 413, 1033
BseMI GCAATG 1 cut(s) 290
BseMII CTCAG 2 cut(s) 270, 402
BseNI ACTGG 1 cut(s) 334
BseSI GKGCMC 1 cut(s) 785
BseXI GCAGC 5 cut(s) 331, 452, 976, 1049, 1191
BsgI GTGCAG 1 cut(s) 939
Bsh1236I CGCG 1 cut(s) 999
BshFI GGCC 2 cut(s) 199, 328
BshNI GGYRCC 5 cut(s) 403, 554, 598, 782, 978
BshVI ATCGAT 1 cut(s) 616
BsiHKAI GWGCWC 1 cut(s) 702
BsiHKCI CYCGRG 3 cut(s) 204, 507, 913
BsiSI CCGG 3 cut(s) 146, 323, 914
BslFI GGGAC 2 cut(s) 263, 1036
BslI CCNNNNNNNGG 2 cut(s) 413, 1033
BsmAI GTCTC 4 cut(s) 342, 766, 841, 951
BsmFI GGGAC 2 cut(s) 263, 1036
BsmI GAATGC 1 cut(s) 738
BsnI GGCC 2 cut(s) 199, 328
BsoBI CYCGRG 3 cut(s) 204, 507, 913
Bsp1286I GDGCHC 2 cut(s) 702, 785
Bsp13I TCCGGA 1 cut(s) 145
Bsp143I GATC 5 cut(s) 450, 617, 859, 1045, 1167
BspACI CCGC 4 cut(s) 43, 596, 786, 1142
BspANI GGCC 2 cut(s) 199, 328
BspCNI CTCAG 2 cut(s) 271, 403
BspDI ATCGAT 1 cut(s) 616
BspEI TCCGGA 1 cut(s) 145
BspFNI CGCG 1 cut(s) 999
BspLI GGNNCC 9 cut(s) 93, 327, 405, 556, 600, 632, 784, 897, 980
BspMI ACCTGC 1 cut(s) 634
BspOI GCTAGC 1 cut(s) 440
BspPI GGATC 3 cut(s) 458, 867, 1040
BspQI GCTCTTC 1 cut(s) 1065
BspT107I GGYRCC 5 cut(s) 403, 554, 598, 782, 978
BspTI CTTAAG 1 cut(s) 1174
BsrBI CCGCTC 1 cut(s) 43
BsrDI GCAATG 1 cut(s) 290
BsrI ACTGG 1 cut(s) 334
BssECI CCNNGG 6 cut(s) 323, 725, 912, 913, 950, 1125
BssMI GATC 5 cut(s) 450, 617, 859, 1045, 1167
BssNAI GTATAC 1 cut(s) 571
BssSI CACGAG 1 cut(s) 193
BssT1I CCWWGG 3 cut(s) 725, 950, 1125
Bst1107I GTATAC 1 cut(s) 571
Bst2BI CACGAG 1 cut(s) 193
Bst4CI ACNGT 4 cut(s) 348, 568, 1006, 1095
Bst6I CTCTTC 1 cut(s) 1065
BstAFI CTTAAG 1 cut(s) 1174
BstC8I GCNNGC 5 cut(s) 201, 438, 514, 581, 1197
BstDEI CTNAG 3 cut(s) 279, 411, 639
BstFNI CGCG 1 cut(s) 999
BstKTI GATC 5 cut(s) 453, 620, 862, 1048, 1170
BstMAI GTCTC 4 cut(s) 342, 766, 841, 951
BstMBI GATC 5 cut(s) 450, 617, 859, 1045, 1167
BstMWI GCNNNNNNNGC 5 cut(s) 325, 518, 917, 964, 1179
BstNSI RCATGY 1 cut(s) 25
BstSCI CCNGG 3 cut(s) 322, 912, 913
BstSFI CTRYAG 2 cut(s) 673, 1091
BstSLI GKGCMC 1 cut(s) 785
BstUI CGCG 1 cut(s) 999
BstV1I GCAGC 5 cut(s) 331, 452, 976, 1049, 1191
BstX2I RGATCY 1 cut(s) 859
BstXI CCANNNNNNTGG 1 cut(s) 550
BstYI RGATCY 1 cut(s) 859
BstZ17I GTATAC 1 cut(s) 571
Bsu15I ATCGAT 1 cut(s) 616
BsuRI GGCC 2 cut(s) 199, 328
BsuTUI ATCGAT 1 cut(s) 616
BtsI GCAGTG 1 cut(s) 523
BtsIMutI CAGTG 4 cut(s) 134, 353, 523, 1011
BveI ACCTGC 1 cut(s) 634
Cac8I GCNNGC 5 cut(s) 201, 438, 514, 581, 1197
Cfr13I GGNCC 3 cut(s) 326, 540, 722
Cfr9I CCCGGG 1 cut(s) 913
ClaI ATCGAT 1 cut(s) 616
CseI GACGC 1 cut(s) 1005
Csp6I GTAC 4 cut(s) 391, 555, 599, 659
CviAII CATG 4 cut(s) 22, 243, 588, 959
CviQI GTAC 4 cut(s) 391, 555, 599, 659
DdeI CTNAG 3 cut(s) 279, 411, 639
DpnI GATC 5 cut(s) 452, 619, 861, 1047, 1169
DpnII GATC 5 cut(s) 450, 617, 859, 1045, 1167
Eam1104I CTCTTC 1 cut(s) 1065
EarI CTCTTC 1 cut(s) 1065
Ecl136II GAGCTC 1 cut(s) 700
Eco130I CCWWGG 3 cut(s) 725, 950, 1125
Eco24I GRGCYC 1 cut(s) 702
Eco47I GGWCC 2 cut(s) 540, 722
Eco53kI GAGCTC 1 cut(s) 700
Eco57I CTGAAG 1 cut(s) 1089
Eco88I CYCGRG 3 cut(s) 204, 507, 913
EcoICRI GAGCTC 1 cut(s) 700
EcoO109I RGGNCCY 1 cut(s) 326
EcoT14I CCWWGG 3 cut(s) 725, 950, 1125
EcoT22I ATGCAT 1 cut(s) 738
EcoT38I GRGCYC 1 cut(s) 702
ErhI CCWWGG 3 cut(s) 725, 950, 1125
FaeI CATG 4 cut(s) 25, 246, 591, 962
FaqI GGGAC 2 cut(s) 263, 1036
FatI CATG 4 cut(s) 21, 242, 587, 958
FauI CCCGC 1 cut(s) 1149
FauNDI CATATG 1 cut(s) 523
FblI GTMKAC 1 cut(s) 570
Fnu4HI GCNGC 5 cut(s) 320, 441, 965, 1038, 1180
FriOI GRGCYC 1 cut(s) 702
Fsp4HI GCNGC 5 cut(s) 320, 441, 965, 1038, 1180
FspBI CTAG 2 cut(s) 437, 951
GluI GCNGC 5 cut(s) 320, 441, 965, 1038, 1180
HaeIII GGCC 2 cut(s) 199, 328
HapII CCGG 3 cut(s) 146, 323, 914
HgaI GACGC 1 cut(s) 1005
Hin1II CATG 4 cut(s) 25, 246, 591, 962
HindIII AAGCTT 1 cut(s) 47
HinfI GANTC 1 cut(s) 115
HpaII CCGG 3 cut(s) 146, 323, 914
HphI GGTGA 5 cut(s) 50, 428, 556, 831, 1114
Hpy166II GTNNAC 2 cut(s) 564, 571
Hpy188I TCNGA 5 cut(s) 171, 412, 455, 496, 895
Hpy188III TCNNGA 6 cut(s) 83, 146, 278, 507, 624, 773
Hpy8I GTNNAC 2 cut(s) 564, 571
HpyAV CCTTC 5 cut(s) 54, 285, 354, 450, 621
HpyCH4III ACNGT 4 cut(s) 348, 568, 1006, 1095
HpyCH4IV ACGT 3 cut(s) 65, 903, 1059
HpyCH4V TGCA 5 cut(s) 521, 714, 736, 748, 920
HpyF10VI GCNNNNNNNGC 5 cut(s) 325, 518, 917, 964, 1179
HpyF3I CTNAG 3 cut(s) 279, 411, 639
HpySE526I ACGT 3 cut(s) 65, 903, 1059
Hsp92II CATG 4 cut(s) 25, 246, 591, 962
Kpn2I TCCGGA 1 cut(s) 145
KpnI GGTACC 2 cut(s) 558, 602
Kzo9I GATC 5 cut(s) 450, 617, 859, 1045, 1167
LguI GCTCTTC 1 cut(s) 1065
LmnI GCTCC 4 cut(s) 18, 85, 97, 895
Lsp1109I GCAGC 5 cut(s) 331, 452, 976, 1049, 1191
LweI GCATC 1 cut(s) 583
MaeI CTAG 2 cut(s) 437, 951
MaeII ACGT 3 cut(s) 65, 903, 1059
MaeIII GTNAC 2 cut(s) 668, 1000
MalI GATC 5 cut(s) 452, 619, 861, 1047, 1169
MbiI CCGCTC 1 cut(s) 43
MboI GATC 5 cut(s) 450, 617, 859, 1045, 1167
MboII GAAGA 3 cut(s) 191, 1082, 1111
MflI RGATCY 1 cut(s) 859
MhlI GDGCHC 2 cut(s) 702, 785
MluCI AATT 6 cut(s) 12, 149, 165, 428, 533, 827
MluI ACGCGT 1 cut(s) 997
MmeI TCCRAC 3 cut(s) 119, 128, 567
Mph1103I ATGCAT 1 cut(s) 738
MroI TCCGGA 1 cut(s) 145
MroXI GAANNNNTTC 1 cut(s) 1022
MseI TTAA 2 cut(s) 984, 1175
MspA1I CMGCKG 2 cut(s) 1040, 1144
MspCI CTTAAG 1 cut(s) 1174
MspI CCGG 3 cut(s) 146, 323, 914
MspR9I CCNGG 3 cut(s) 324, 914, 915
Mva1269I GAATGC 1 cut(s) 738
MvnI CGCG 1 cut(s) 999
MwoI GCNNNNNNNGC 5 cut(s) 325, 518, 917, 964, 1179
NciI CCSGG 3 cut(s) 324, 914, 915
NdeI CATATG 1 cut(s) 523
NdeII GATC 5 cut(s) 450, 617, 859, 1045, 1167
NheI GCTAGC 1 cut(s) 436
NlaIII CATG 4 cut(s) 25, 246, 591, 962
NlaIV GGNNCC 9 cut(s) 93, 327, 405, 556, 600, 632, 784, 897, 980
NsiI ATGCAT 1 cut(s) 738
NspI RCATGY 1 cut(s) 25
PaeR7I CTCGAG 2 cut(s) 204, 507
PciSI GCTCTTC 1 cut(s) 1065
PcsI WCGNNNNNNNCGW 1 cut(s) 622
PctI GAATGC 1 cut(s) 738
PdmI GAANNNNTTC 1 cut(s) 1022
PfeI GAWTC 1 cut(s) 115
PflFI GACNNNGTC 1 cut(s) 67
PflMI CCANNNNNTGG 1 cut(s) 1033
PkrI GCNGC 5 cut(s) 321, 442, 966, 1039, 1181
Psp124BI GAGCTC 1 cut(s) 702
Psp1406I AACGTT 1 cut(s) 1059
PspN4I GGNNCC 9 cut(s) 93, 327, 405, 556, 600, 632, 784, 897, 980
PspPI GGNCC 3 cut(s) 326, 540, 722
PspXI VCTCGAGB 1 cut(s) 204
PsuI RGATCY 1 cut(s) 859
PsyI GACNNNGTC 1 cut(s) 67
PvuII CAGCTG 1 cut(s) 1040
RsaI GTAC 4 cut(s) 392, 556, 600, 660
RsaNI GTAC 4 cut(s) 391, 555, 599, 659
SacI GAGCTC 1 cut(s) 702
SapI GCTCTTC 1 cut(s) 1065
SaqAI TTAA 2 cut(s) 984, 1175
SatI GCNGC 5 cut(s) 320, 441, 965, 1038, 1180
Sau3AI GATC 5 cut(s) 450, 617, 859, 1045, 1167
Sau96I GGNCC 3 cut(s) 326, 540, 722
ScrFI CCNGG 3 cut(s) 324, 914, 915
SduI GDGCHC 2 cut(s) 702, 785
SfaNI GCATC 1 cut(s) 583
SfcI CTRYAG 2 cut(s) 673, 1091
Sfr274I CTCGAG 2 cut(s) 204, 507
SinI GGWCC 2 cut(s) 540, 722
SlaI CTCGAG 2 cut(s) 204, 507
SmaI CCCGGG 1 cut(s) 915
SmlI CTYRAG 3 cut(s) 204, 507, 1174
SmoI CTYRAG 3 cut(s) 204, 507, 1174
Sse9I AATT 6 cut(s) 12, 149, 165, 428, 533, 827
SsiI CCGC 4 cut(s) 43, 596, 786, 1142
SspI AATATT 1 cut(s) 706
SspMI CTAG 2 cut(s) 437, 951
SstI GAGCTC 1 cut(s) 702
StyD4I CCNGG 3 cut(s) 322, 912, 913
StyI CCWWGG 3 cut(s) 725, 950, 1125
TaaI ACNGT 4 cut(s) 348, 568, 1006, 1095
TaiI ACGT 3 cut(s) 68, 906, 1062
TaqI TCGA 5 cut(s) 205, 508, 616, 625, 1185
TasI AATT 6 cut(s) 12, 149, 165, 428, 533, 827
TatI WGTACW 1 cut(s) 390
TfiI GAWTC 1 cut(s) 115
Tru1I TTAA 2 cut(s) 984, 1175
Tru9I TTAA 2 cut(s) 984, 1175
TscAI CASTG 4 cut(s) 134, 353, 523, 1011
TseI GCWGC 5 cut(s) 319, 440, 964, 1037, 1179
TspDTI ATGAA 2 cut(s) 17, 343
TspGWI ACGGA 2 cut(s) 485, 1038
TspMI CCCGGG 1 cut(s) 913
TspRI CASTG 4 cut(s) 134, 353, 523, 1011
Tth111I GACNNNGTC 1 cut(s) 67
Van91I CCANNNNNTGG 1 cut(s) 1033
Vha464I CTTAAG 1 cut(s) 1174
VpaK11BI GGWCC 2 cut(s) 540, 722
XceI RCATGY 1 cut(s) 25
XhoI CTCGAG 2 cut(s) 204, 507
XmaI CCCGGG 1 cut(s) 913
XmaJI CCTAGG 1 cut(s) 950
XmiI GTMKAC 1 cut(s) 570
XmnI GAANNNNTTC 1 cut(s) 1022
XspI CTAG 2 cut(s) 437, 951
Zsp2I ATGCAT 1 cut(s) 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.