MD02G1182200.v1.1
ERF Family

Major facilitator superfamily domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0007474
Physical Location & Seq
Reverse (-)
16423912 .. 16427099
3188 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1182200.v1.1.491

Sequence Viewer

Length: 1377 bp
ATGGCGGTGGTGATTGAGAGCTCCGTTTGGGAACCGAATCCCGGCGTATACATATTCATCTTCCTGGCCTCACTCTTCTCCATCCTCGTCTTCCCTTACGCTTGCAGCAGCAGCGGCGGCGGCAGCTCTGGCAAAGCTCCGTCGCTCTTCGACCATGGAATCTCCCCCACCGCTTCCTCTCTTCGCTTCCAGCGCAACTTCCTTCTCATCTATTCTCTCGCCTCAGTGATGGAAGGATTGTGGTCTGTATTTGGCGAGTTCGAATTAGCTTACTACGGTTTCAGCAGAGAGCAAATGATGTTTTCTCTCTGTGTTGGATTTGCTGCGTCTCTGTGTGTTGGTTCTTTATTTGGCGTGCTTTCGGATTTAATAGGTCCAAAGAAAGTTTGCATCCTCTTTTGCATTCTGCACCTTTTTCTTGGCTGGTGGAAGAGAATTACTGATCATCCGAGCATTTTGTTGGCGAGAATTTGTTTGTCACTTGCAGCTTCAATTTTTTCATTCAGTTTTGAGACGTGGATGGTGGTTCAACACGAAGAGCAAGGACATAGGCAGGATATGTTGAGTGAGACGTTTTGGTTAATGAGTTTCTTTGAGTCCGCGTTTCTTATTGGAAGCCAGGTGCTTTCAAATTGGCTCATTGGTGATAATTTGGAGAAAAACATGGCTTCACATTCTACTGCAGCCATCTTCTTGGCAATGGTAGGCTTAGTCTGCCTCCTCAGAGGATGGACACAAACCCCACAAAAAGTAGCTCTTAAGGAGTATAGAGCGTCTTTCTCTGCATATATTTTTGGTGATAAAAGAATATGGCTTTTGGCATGGGCACAAGCTTGTCTTCACTTCTCTATAACAGTCTTCTGGATACTATGGGCCCCTACAATAGTGGCCGATGGACGGGAAGTGCACCTAGGGTTGATATATCCATGTTTTATGGGCTCAAGAATGCTTGGAAGCACAGTATTTCCATGGCTGAATAGTGGACTGTCGTCCCTTAGAACTGAGGATTGCCTAGTATATGCATTCATCATGCTGGGGCTTGTATTGTCCGTAACAGCTTATGATTACCAGGAAATTGGAGTTTTAACGACACTATTTTGCATATTTCACGCTGGTCTTGGTGTGGTTTTGCCTTCACTTGCCAGATTGAGGACCATGTATGTCCCTAATGCACTGCGTGGAGGGATGATAAGCCTTTCTCAAGCTCCTGCAAATGCAGCAATTCTGCTTTTTCTGGTGCAAGGAAAATACTACTATAACATTGGGAATTCAACAATCATAGCGTTTGCTGCGCTTGGGCTATTCACAGCAGCTGGTTGCATGCATGTCTTGAAGCGATGGGGGAAGCAACCGTATCACAACTGGCACAAATTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_5

Protein Analysis

459

Amino Acids

50.99

Weight (kDa)

8.21

Isoelectric Point (pI)

45.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_5 PF05631 62 - 351 7.2e-31 Sugar-tranasporters, 12 TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 48
AccII CGCG 1 cut(s) 602
AciI CCGC 6 cut(s) 5, 114, 117, 120, 171, 600
AcoI YGGCCR 1 cut(s) 888
AcsI RAATTY 2 cut(s) 468, 1267
AdeI CACNNNGTG 1 cut(s) 1178
AfiI CCNNNNNNNGG 3 cut(s) 41, 897, 1149
AflII CTTAAG 1 cut(s) 758
AgsI TTSAA 5 cut(s) 492, 530, 630, 1272, 1333
AjiI CACGTC 1 cut(s) 516
AjnI CCWGG 3 cut(s) 63, 618, 1068
Alw21I GWGCWC 2 cut(s) 23, 909
Alw26I GTCTC 3 cut(s) 333, 506, 563
Alw44I GTGCAC 1 cut(s) 905
AlwNI CAGNNNCTG 1 cut(s) 1313
AoxI GGCC 3 cut(s) 66, 873, 888
ApaI GGGCCC 1 cut(s) 877
ApaLI GTGCAC 1 cut(s) 905
ApoI RAATTY 2 cut(s) 468, 1267
AspA2I CCTAGG 1 cut(s) 910
AspLEI GCGC 2 cut(s) 195, 1294
AspS9I GGNCC 4 cut(s) 374, 873, 874, 1152
AsuC2I CCSGG 1 cut(s) 42
AsuHPI GGTGA 3 cut(s) 22, 656, 809
AsuII TTCGAA 1 cut(s) 261
AvaII GGWCC 2 cut(s) 374, 1152
AvrII CCTAGG 1 cut(s) 910
BaeGI GKGCMC 3 cut(s) 829, 877, 909
BanII GRGCYC 3 cut(s) 23, 877, 941
BbsI GAAGAC 3 cut(s) 82, 830, 850
Bbv12I GWGCWC 2 cut(s) 23, 909
BccI CCATC 7 cut(s) 89, 223, 514, 695, 723, 887, 1332
BciT130I CCWGG 3 cut(s) 65, 620, 1070
BciVI GTATCC 1 cut(s) 858
BclI TGATCA 1 cut(s) 442
BcnI CCSGG 1 cut(s) 42
BcoDI GTCTC 3 cut(s) 333, 506, 563
BfaI CTAG 2 cut(s) 911, 1013
BfmI CTRYAG 1 cut(s) 681
BfrI CTTAAG 1 cut(s) 758
BfuI GTATCC 1 cut(s) 858
BlnI CCTAGG 1 cut(s) 910
Bme1390I CCNGG 4 cut(s) 42, 65, 620, 1070
Bme18I GGWCC 2 cut(s) 374, 1152
BmgBI CACGTC 1 cut(s) 516
BmgT120I GGNCC 4 cut(s) 374, 873, 874, 1152
BmiI GGNNCC 3 cut(s) 33, 875, 876
BmrFI CCNGG 4 cut(s) 42, 65, 620, 1070
BmsI GCATC 1 cut(s) 399
BoxI GACNNNNGTC 1 cut(s) 988
BpiI GAAGAC 3 cut(s) 82, 830, 850
Bpu14I TTCGAA 1 cut(s) 261
BpuEI CTTGAG 2 cut(s) 925, 1185
BpuMI CCSGG 1 cut(s) 42
BsaJI CCNNGG 3 cut(s) 154, 910, 968
Bsc4I CCNNNNNNNGG 3 cut(s) 41, 897, 1149
Bse1I ACTGG 1 cut(s) 1367
Bse3DI GCAATG 1 cut(s) 705
BseBI CCWGG 3 cut(s) 65, 620, 1070
BseDI CCNNGG 3 cut(s) 154, 910, 968
BseGI GGATG 6 cut(s) 81, 390, 445, 525, 734, 1191
BseLI CCNNNNNNNGG 3 cut(s) 41, 897, 1149
BseMI GCAATG 1 cut(s) 705
BseMII CTCAG 3 cut(s) 237, 736, 993
BseNI ACTGG 1 cut(s) 1367
BseRI GAGGAG 1 cut(s) 710
BseSI GKGCMC 3 cut(s) 829, 877, 909
BseYI CCCAGC 1 cut(s) 1033
BsgI GTGCAG 1 cut(s) 392
Bsh1236I CGCG 1 cut(s) 602
BshFI GGCC 3 cut(s) 68, 875, 890
BsiHKAI GWGCWC 2 cut(s) 23, 909
BsiSI CCGG 1 cut(s) 42
BslFI GGGAC 2 cut(s) 976, 1148
BslI CCNNNNNNNGG 3 cut(s) 41, 897, 1149
BsmAI GTCTC 3 cut(s) 333, 506, 563
BsmBI CGTCTC 3 cut(s) 333, 506, 563
BsmFI GGGAC 2 cut(s) 976, 1148
BsmI GAATGC 3 cut(s) 402, 951, 1022
BsnI GGCC 3 cut(s) 68, 875, 890
Bsp119I TTCGAA 1 cut(s) 261
Bsp120I GGGCCC 1 cut(s) 873
Bsp1286I GDGCHC 5 cut(s) 23, 829, 877, 909, 941
Bsp143I GATC 1 cut(s) 442
Bsp19I CCATGG 2 cut(s) 154, 968
BspACI CCGC 6 cut(s) 5, 114, 117, 120, 171, 600
BspANI GGCC 3 cut(s) 68, 875, 890
BspCNI CTCAG 3 cut(s) 236, 735, 994
BspFNI CGCG 1 cut(s) 602
BspLI GGNNCC 3 cut(s) 33, 875, 876
BspMAI CTGCAG 1 cut(s) 685
BspQI GCTCTTC 2 cut(s) 152, 531
BspT104I TTCGAA 1 cut(s) 261
BspTI CTTAAG 1 cut(s) 758
BsrDI GCAATG 1 cut(s) 705
BsrI ACTGG 1 cut(s) 1367
BssECI CCNNGG 3 cut(s) 154, 910, 968
BssMI GATC 1 cut(s) 442
BssNAI GTATAC 1 cut(s) 49
BssT1I CCWWGG 3 cut(s) 154, 910, 968
Bst1107I GTATAC 1 cut(s) 49
Bst2UI CCWGG 3 cut(s) 65, 620, 1070
Bst4CI ACNGT 5 cut(s) 278, 856, 961, 987, 1353
Bst6I CTCTTC 5 cut(s) 80, 152, 186, 425, 531
BstAFI CTTAAG 1 cut(s) 758
BstBI TTCGAA 1 cut(s) 261
BstC8I GCNNGC 3 cut(s) 103, 356, 1322
BstDEI CTNAG 5 cut(s) 223, 709, 722, 995, 1002
BstDSI CCRYGG 2 cut(s) 154, 968
BstF5I GGATG 6 cut(s) 81, 390, 445, 525, 734, 1191
BstFNI CGCG 1 cut(s) 602
BstHHI GCGC 2 cut(s) 195, 1294
BstKTI GATC 1 cut(s) 445
BstMAI GTCTC 3 cut(s) 333, 506, 563
BstMBI GATC 1 cut(s) 442
BstNI CCWGG 3 cut(s) 65, 620, 1070
BstNSI RCATGY 2 cut(s) 1324, 1328
BstPAI GACNNNNGTC 1 cut(s) 988
BstSCI CCNGG 4 cut(s) 40, 63, 618, 1068
BstSFI CTRYAG 1 cut(s) 681
BstSLI GKGCMC 3 cut(s) 829, 877, 909
BstUI CGCG 1 cut(s) 602
BstV2I GAAGAC 3 cut(s) 82, 830, 850
BstXI CCANNNNNNTGG 2 cut(s) 694, 1076
BstZ17I GTATAC 1 cut(s) 49
BsuI GTATCC 1 cut(s) 858
BsuRI GGCC 3 cut(s) 68, 875, 890
BtgI CCRYGG 2 cut(s) 154, 968
BtgZI GCGATG 1 cut(s) 1351
BtrI CACGTC 1 cut(s) 516
BtsCI GGATG 6 cut(s) 81, 390, 445, 525, 734, 1191
BtsI GCAGTG 1 cut(s) 1172
BtsIMutI CAGTG 2 cut(s) 231, 1172
Cac8I GCNNGC 3 cut(s) 103, 356, 1322
CaiI CAGNNNCTG 1 cut(s) 1313
CfoI GCGC 2 cut(s) 195, 1294
Cfr13I GGNCC 4 cut(s) 374, 873, 874, 1152
CseI GACGC 2 cut(s) 315, 762
CviAII CATG 9 cut(s) 155, 664, 822, 927, 969, 1030, 1156, 1321, 1325
DdeI CTNAG 5 cut(s) 223, 709, 722, 995, 1002
DpnI GATC 1 cut(s) 444
DpnII GATC 1 cut(s) 442
DraIII CACNNNGTG 1 cut(s) 1178
EaeI YGGCCR 1 cut(s) 888
Eam1104I CTCTTC 5 cut(s) 80, 152, 186, 425, 531
EarI CTCTTC 5 cut(s) 80, 152, 186, 425, 531
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 3 cut(s) 154, 910, 968
Eco24I GRGCYC 3 cut(s) 23, 877, 941
Eco47I GGWCC 2 cut(s) 374, 1152
Eco53kI GAGCTC 1 cut(s) 21
EcoICRI GAGCTC 1 cut(s) 21
EcoO109I RGGNCCY 1 cut(s) 874
EcoRI GAATTC 1 cut(s) 1267
EcoRII CCWGG 3 cut(s) 63, 618, 1068
EcoT14I CCWWGG 3 cut(s) 154, 910, 968
EcoT22I ATGCAT 2 cut(s) 1024, 1326
EcoT38I GRGCYC 3 cut(s) 23, 877, 941
ErhI CCWWGG 3 cut(s) 154, 910, 968
Esp3I CGTCTC 3 cut(s) 333, 506, 563
FaeI CATG 9 cut(s) 158, 667, 825, 930, 972, 1033, 1159, 1324, 1328
FalI AAGNNNNNCTT 4 cut(s) 741, 773, 822, 854
FaqI GGGAC 2 cut(s) 976, 1148
FatI CATG 9 cut(s) 154, 663, 821, 926, 968, 1029, 1155, 1320, 1324
FbaI TGATCA 1 cut(s) 442
FblI GTMKAC 1 cut(s) 48
FokI GGATG 6 cut(s) 68, 377, 432, 532, 741, 1198
FriOI GRGCYC 3 cut(s) 23, 877, 941
FspBI CTAG 2 cut(s) 911, 1013
GlaI GCGC 2 cut(s) 194, 1293
GsaI CCCAGC 1 cut(s) 1037
HaeIII GGCC 3 cut(s) 68, 875, 890
HapII CCGG 1 cut(s) 42
HgaI GACGC 2 cut(s) 315, 762
HhaI GCGC 2 cut(s) 195, 1294
Hin1II CATG 9 cut(s) 158, 667, 825, 930, 972, 1033, 1159, 1324, 1328
Hin6I GCGC 2 cut(s) 193, 1292
HinP1I GCGC 2 cut(s) 193, 1292
HindIII AAGCTT 1 cut(s) 831
HinfI GANTC 3 cut(s) 37, 159, 596
HpaII CCGG 1 cut(s) 42
HphI GGTGA 3 cut(s) 22, 656, 809
Hpy166II GTNNAC 3 cut(s) 49, 907, 983
Hpy188I TCNGA 3 cut(s) 364, 450, 725
Hpy188III TCNNGA 3 cut(s) 862, 942, 1330
Hpy8I GTNNAC 3 cut(s) 49, 907, 983
Hpy99I CGWCG 1 cut(s) 145
HpyAV CCTTC 3 cut(s) 212, 227, 1143
HpyCH4III ACNGT 5 cut(s) 278, 856, 961, 987, 1353
HpyCH4IV ACGT 2 cut(s) 515, 572
HpyF3I CTNAG 5 cut(s) 223, 709, 722, 995, 1002
HpySE526I ACGT 2 cut(s) 515, 572
Hsp92II CATG 9 cut(s) 158, 667, 825, 930, 972, 1033, 1159, 1324, 1328
HspAI GCGC 2 cut(s) 193, 1292
Ksp22I TGATCA 1 cut(s) 442
Kzo9I GATC 1 cut(s) 442
LguI GCTCTTC 2 cut(s) 152, 531
LmnI GCTCC 3 cut(s) 26, 142, 1210
LweI GCATC 1 cut(s) 399
MaeI CTAG 2 cut(s) 911, 1013
MaeII ACGT 2 cut(s) 515, 572
MaeIII GTNAC 2 cut(s) 477, 1051
MalI GATC 1 cut(s) 444
MboI GATC 1 cut(s) 442
MhlI GDGCHC 5 cut(s) 23, 829, 877, 909, 941
MlyI GAGTC 1 cut(s) 605
MmeI TCCRAC 1 cut(s) 295
Mph1103I ATGCAT 2 cut(s) 1024, 1326
MseI TTAA 4 cut(s) 368, 581, 759, 1085
MspA1I CMGCKG 2 cut(s) 114, 1313
MspCI CTTAAG 1 cut(s) 758
MspI CCGG 1 cut(s) 42
MspR9I CCNGG 4 cut(s) 42, 65, 620, 1070
Mva1269I GAATGC 3 cut(s) 402, 951, 1022
MvaI CCWGG 3 cut(s) 65, 620, 1070
MvnI CGCG 1 cut(s) 602
NciI CCSGG 1 cut(s) 42
NcoI CCATGG 2 cut(s) 154, 968
NdeII GATC 1 cut(s) 442
NlaIII CATG 9 cut(s) 158, 667, 825, 930, 972, 1033, 1159, 1324, 1328
NlaIV GGNNCC 3 cut(s) 33, 875, 876
NmuCI GTSAC 1 cut(s) 477
NsiI ATGCAT 2 cut(s) 1024, 1326
NspI RCATGY 2 cut(s) 1324, 1328
NspV TTCGAA 1 cut(s) 261
PaeI GCATGC 1 cut(s) 1324
PciSI GCTCTTC 2 cut(s) 152, 531
PctI GAATGC 3 cut(s) 402, 951, 1022
PfeI GAWTC 2 cut(s) 37, 159
PleI GAGTC 1 cut(s) 604
PpsI GAGTC 1 cut(s) 604
PshAI GACNNNNGTC 1 cut(s) 988
Psp124BI GAGCTC 1 cut(s) 23
Psp6I CCWGG 3 cut(s) 63, 618, 1068
PspFI CCCAGC 1 cut(s) 1033
PspGI CCWGG 3 cut(s) 63, 618, 1068
PspN4I GGNNCC 3 cut(s) 33, 875, 876
PspOMI GGGCCC 1 cut(s) 873
PspPI GGNCC 4 cut(s) 374, 873, 874, 1152
PstI CTGCAG 1 cut(s) 685
PstNI CAGNNNCTG 1 cut(s) 1313
PvuII CAGCTG 1 cut(s) 1313
SacI GAGCTC 1 cut(s) 23
SapI GCTCTTC 2 cut(s) 152, 531
SaqAI TTAA 4 cut(s) 368, 581, 759, 1085
Sau3AI GATC 1 cut(s) 442
Sau96I GGNCC 4 cut(s) 374, 873, 874, 1152
SchI GAGTC 1 cut(s) 605
ScrFI CCNGG 4 cut(s) 42, 65, 620, 1070
SduI GDGCHC 5 cut(s) 23, 829, 877, 909, 941
SfaNI GCATC 1 cut(s) 399
SfcI CTRYAG 1 cut(s) 681
SfuI TTCGAA 1 cut(s) 261
SinI GGWCC 2 cut(s) 374, 1152
SmlI CTYRAG 3 cut(s) 758, 940, 1200
SmoI CTYRAG 3 cut(s) 758, 940, 1200
SphI GCATGC 1 cut(s) 1324
SsiI CCGC 6 cut(s) 5, 114, 117, 120, 171, 600
SspMI CTAG 2 cut(s) 911, 1013
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 4 cut(s) 40, 63, 618, 1068
StyI CCWWGG 3 cut(s) 154, 910, 968
TaaI ACNGT 5 cut(s) 278, 856, 961, 987, 1353
TaiI ACGT 2 cut(s) 518, 575
TaqI TCGA 2 cut(s) 150, 261
TauI GCSGC 3 cut(s) 117, 120, 123
TfiI GAWTC 2 cut(s) 37, 159
Tru1I TTAA 4 cut(s) 368, 581, 759, 1085
Tru9I TTAA 4 cut(s) 368, 581, 759, 1085
TscAI CASTG 2 cut(s) 231, 1179
TseFI GTSAC 1 cut(s) 477
Tsp45I GTSAC 1 cut(s) 477
TspDTI ATGAA 3 cut(s) 46, 489, 1015
TspGWI ACGGA 3 cut(s) 13, 129, 1039
TspRI CASTG 2 cut(s) 231, 1179
Vha464I CTTAAG 1 cut(s) 758
VneI GTGCAC 1 cut(s) 905
VpaK11BI GGWCC 2 cut(s) 374, 1152
XapI RAATTY 2 cut(s) 468, 1267
XceI RCATGY 2 cut(s) 1324, 1328
XmaJI CCTAGG 1 cut(s) 910
XmiI GTMKAC 1 cut(s) 48
XspI CTAG 2 cut(s) 911, 1013
Zsp2I ATGCAT 2 cut(s) 1024, 1326
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.