Rh2BG205500
ERF Family

Major facilitator superfamily domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0007474
Physical Location & Seq
Reverse (-)
18986272 .. 18990210
3939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG205500.1

Sequence Viewer

Length: 1164 bp
ATGGCGGTGGTGATTGAGAGCTCCGTTTGGGAACCGAGCCCCGCGCTTTACATCTTCATCTTCTTCGCCTGCCTCTTCTCGATTCTCTTCTTCCCTTACGCCTCCGGCAGCACCAACAGCACCTCCGCCGCGAAAGCTGCTCCGTCTCTGTTCGACCATGGAGTCTCCCCCACCGCTTCGTCTTTTCGGTTCCAGAGGAACTTCCTGCTTCTCTATTCTCTCGCCTCAGTTATGGAAGGACTGTGGTCGGTGTTCGGCGAAGTTGAGCTAGCTTACTACGGATTAAGCAAAGAGAAAATGGTGCTTTATCTCTGCATAGGACTTGCGGCTTCGATTTCTATCGGTTCTTTCTTTGGAGTACTTTCTGATTTAATAGGTCCAAAGAAAGTTTGCTTCTTCTTTTGCTTTCTACACCTCCTTGTTGGCTTGTTGAAAAGAATCGCCCCTCATTCGAGTGTTTGGGTGGCGAGTATTTGTTTGTCAATCACAAGTTCAGTTTTTTCATTCGGTTTCGAGACGTGGATGGTGGTTCAACATGAAGAGCAAGGGCACAGGCAAGATATGCTGAGTGATACATTTTGGTTAATGAGTTTCTTCGAGTCAGCATGTCTTATTGGTAGCCAGGTTTTTTCAAATTGGCTCATCGGTAATAATGCAGAGAGAAGTATGGCGTCTCATTCCACTGCAGCTATCTTCTTGGCAGTAATAAGCTTAGTCTGTCTCACCAAGGGATGGAAAGAAATCCCACATAAAGTAGCTCTTAAGGAGTATAGGGCATCTTTCCGTGCATACATTGTTGGCGATAAAAGACTATGGCTTTTGGCATGGGCACAAGCTTGCCTTCACTTCTCTGTTGCAGTATTCTGGATACTATGGGCCCCAACAATAGTGGCTGATGGACGAGAAGTGCACCTAGGGTTGATATATCCATGTTTTCTTGGATCAAGAATGCTTGGAAGCACAGTATTCCCATGGCTCATTAGTGGACTGTCATCCCTTAGAACTGATGATTGCCTAGTATATGCATTCGTTGTACTGGGGCTAGTAATGTCCATAACAGCTTATGATTATCAGGAAGTTGGGATGCTAGTGACGCTATTTTGCATATTTCATGCCGGCCTTGGCGTGGTTTTGCCTTCACTTGCCAGATTGAGAACCATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_5

Protein Analysis

387

Amino Acids

42.98

Weight (kDa)

7.11

Isoelectric Point (pI)

41.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_5 PF05631 63 - 352 7.5e-26 Sugar-tranasporters, 12 TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 161
AccB7I CCANNNNNTGG 1 cut(s) 732
AccII CGCG 2 cut(s) 44, 131
AciI CCGC 6 cut(s) 5, 42, 126, 129, 174, 326
AclWI GGATC 1 cut(s) 949
AcyI GRCGYC 1 cut(s) 671
AfaI GTAC 2 cut(s) 360, 1035
AfiI CCNNNNNNNGG 2 cut(s) 732, 1126
AflII CTTAAG 1 cut(s) 761
AgsI TTSAA 3 cut(s) 433, 533, 633
AjiI CACGTC 1 cut(s) 519
AjnI CCWGG 1 cut(s) 621
AluBI AGCT 9 cut(s) 21, 137, 268, 272, 689, 711, 758, 836, 1061
AluI AGCT 9 cut(s) 21, 137, 268, 272, 689, 711, 758, 836, 1061
Alw21I GWGCWC 2 cut(s) 23, 912
Alw26I GTCTC 5 cut(s) 150, 169, 509, 678, 725
Alw44I GTGCAC 1 cut(s) 908
AlwI GGATC 1 cut(s) 949
AoxI GGCC 2 cut(s) 876, 1117
ApaI GGGCCC 1 cut(s) 880
ApaLI GTGCAC 1 cut(s) 908
ApeKI GCWGC 3 cut(s) 108, 137, 686
AspA2I CCTAGG 1 cut(s) 913
AspLEI GCGC 1 cut(s) 46
AspS9I GGNCC 3 cut(s) 377, 876, 877
AsuHPI GGTGA 2 cut(s) 22, 715
AsuNHI GCTAGC 1 cut(s) 268
AvaII GGWCC 1 cut(s) 377
AvrII CCTAGG 1 cut(s) 913
BaeGI GKGCMC 4 cut(s) 552, 832, 880, 912
BanII GRGCYC 3 cut(s) 23, 41, 880
Bbv12I GWGCWC 2 cut(s) 23, 912
BbvI GCAGC 3 cut(s) 120, 124, 698
BccI CCATC 3 cut(s) 517, 726, 890
BciT130I CCWGG 1 cut(s) 623
BciVI GTATCC 1 cut(s) 861
BcoDI GTCTC 5 cut(s) 150, 169, 509, 678, 725
BfaI CTAG 5 cut(s) 269, 914, 1016, 1043, 1088
BfmI CTRYAG 1 cut(s) 684
BfrI CTTAAG 1 cut(s) 761
BfuI GTATCC 1 cut(s) 861
BisI GCNGC 5 cut(s) 109, 129, 138, 327, 687
BlnI CCTAGG 1 cut(s) 913
BlsI GCNGC 5 cut(s) 110, 130, 139, 328, 688
BmcAI AGTACT 1 cut(s) 360
Bme1390I CCNGG 1 cut(s) 623
Bme18I GGWCC 1 cut(s) 377
BmgBI CACGTC 1 cut(s) 519
BmgT120I GGNCC 3 cut(s) 377, 876, 877
BmiI GGNNCC 4 cut(s) 33, 191, 878, 879
BmrFI CCNGG 1 cut(s) 623
BmrI ACTGGG 1 cut(s) 1046
BmsI GCATC 2 cut(s) 785, 1074
BmtI GCTAGC 1 cut(s) 272
BmuI ACTGGG 1 cut(s) 1046
BoxI GACNNNNGTC 1 cut(s) 244
BsaBI GATNNNNATC 1 cut(s) 338
BsaHI GRCGYC 1 cut(s) 671
BsaJI CCNNGG 5 cut(s) 157, 726, 913, 971, 1120
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 2 cut(s) 732, 1126
Bse118I RCCGGY 1 cut(s) 1115
Bse1I ACTGG 1 cut(s) 1041
Bse8I GATNNNNATC 1 cut(s) 338
BseBI CCWGG 1 cut(s) 623
BseDI CCNNGG 5 cut(s) 157, 726, 913, 971, 1120
BseGI GGATG 4 cut(s) 528, 737, 992, 1089
BseJI GATNNNNATC 1 cut(s) 338
BseLI CCNNNNNNNGG 2 cut(s) 732, 1126
BseMII CTCAG 2 cut(s) 240, 557
BseNI ACTGG 1 cut(s) 1041
BseSI GKGCMC 4 cut(s) 552, 832, 880, 912
BseXI GCAGC 3 cut(s) 120, 124, 698
Bsh1236I CGCG 2 cut(s) 44, 131
BshFI GGCC 2 cut(s) 878, 1119
BsiHKAI GWGCWC 2 cut(s) 23, 912
BsiSI CCGG 2 cut(s) 105, 1116
BslI CCNNNNNNNGG 2 cut(s) 732, 1126
BsmAI GTCTC 5 cut(s) 150, 169, 509, 678, 725
BsmBI CGTCTC 3 cut(s) 150, 509, 678
BsmI GAATGC 2 cut(s) 954, 1025
BsnI GGCC 2 cut(s) 878, 1119
Bsp120I GGGCCC 1 cut(s) 876
Bsp1286I GDGCHC 6 cut(s) 23, 41, 552, 832, 880, 912
Bsp143I GATC 1 cut(s) 941
Bsp19I CCATGG 2 cut(s) 157, 971
BspACI CCGC 6 cut(s) 5, 42, 126, 129, 174, 326
BspANI GGCC 2 cut(s) 878, 1119
BspCNI CTCAG 2 cut(s) 239, 558
BspFNI CGCG 2 cut(s) 44, 131
BspLI GGNNCC 4 cut(s) 33, 191, 878, 879
BspMAI CTGCAG 1 cut(s) 688
BspOI GCTAGC 1 cut(s) 272
BspPI GGATC 1 cut(s) 949
BspQI GCTCTTC 1 cut(s) 534
BspTI CTTAAG 1 cut(s) 761
BsrFI RCCGGY 1 cut(s) 1115
BsrI ACTGG 1 cut(s) 1041
BssAI RCCGGY 1 cut(s) 1115
BssECI CCNNGG 5 cut(s) 157, 726, 913, 971, 1120
BssMI GATC 1 cut(s) 941
BssNI GRCGYC 1 cut(s) 671
BssT1I CCWWGG 5 cut(s) 157, 726, 913, 971, 1120
Bst2UI CCWGG 1 cut(s) 623
Bst4CI ACNGT 3 cut(s) 243, 964, 990
Bst6I CTCTTC 3 cut(s) 80, 92, 534
BstACI GRCGYC 1 cut(s) 671
BstAFI CTTAAG 1 cut(s) 761
BstAPI GCANNNNNTGC 1 cut(s) 562
BstC8I GCNNGC 4 cut(s) 70, 270, 838, 1117
BstDEI CTNAG 4 cut(s) 226, 566, 712, 998
BstDSI CCRYGG 2 cut(s) 157, 971
BstF5I GGATG 4 cut(s) 528, 737, 992, 1089
BstFNI CGCG 2 cut(s) 44, 131
BstHHI GCGC 1 cut(s) 46
BstKTI GATC 1 cut(s) 944
BstMAI GTCTC 5 cut(s) 150, 169, 509, 678, 725
BstMBI GATC 1 cut(s) 941
BstMWI GCNNNNNNNGC 5 cut(s) 117, 134, 137, 562, 1093
BstNI CCWGG 1 cut(s) 623
BstNSI RCATGY 1 cut(s) 609
BstPAI GACNNNNGTC 1 cut(s) 244
BstSCI CCNGG 1 cut(s) 621
BstSFI CTRYAG 1 cut(s) 684
BstSLI GKGCMC 4 cut(s) 552, 832, 880, 912
BstUI CGCG 2 cut(s) 44, 131
BstV1I GCAGC 3 cut(s) 120, 124, 698
BsuI GTATCC 1 cut(s) 861
BsuRI GGCC 2 cut(s) 878, 1119
BtgI CCRYGG 2 cut(s) 157, 971
BtrI CACGTC 1 cut(s) 519
BtsCI GGATG 4 cut(s) 528, 737, 992, 1089
BtsI GCAGTG 1 cut(s) 681
BtsIMutI CAGTG 1 cut(s) 681
Cac8I GCNNGC 4 cut(s) 70, 270, 838, 1117
CfoI GCGC 1 cut(s) 46
Cfr10I RCCGGY 1 cut(s) 1115
Cfr13I GGNCC 3 cut(s) 377, 876, 877
CseI GACGC 2 cut(s) 660, 1102
Csp6I GTAC 2 cut(s) 359, 1034
CspCI CAANNNNNGTGG 2 cut(s) 870, 905
CviAII CATG 8 cut(s) 158, 536, 606, 825, 930, 972, 1112, 1159
CviQI GTAC 2 cut(s) 359, 1034
DdeI CTNAG 4 cut(s) 226, 566, 712, 998
DpnI GATC 1 cut(s) 943
DpnII GATC 1 cut(s) 941
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
Eam1104I CTCTTC 3 cut(s) 80, 92, 534
EarI CTCTTC 3 cut(s) 80, 92, 534
EciI GGCGGA 1 cut(s) 115
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 5 cut(s) 157, 726, 913, 971, 1120
Eco24I GRGCYC 3 cut(s) 23, 41, 880
Eco47I GGWCC 1 cut(s) 377
Eco53kI GAGCTC 1 cut(s) 21
EcoICRI GAGCTC 1 cut(s) 21
EcoO109I RGGNCCY 1 cut(s) 877
EcoRII CCWGG 1 cut(s) 621
EcoT14I CCWWGG 5 cut(s) 157, 726, 913, 971, 1120
EcoT22I ATGCAT 1 cut(s) 1027
EcoT38I GRGCYC 3 cut(s) 23, 41, 880
ErhI CCWWGG 5 cut(s) 157, 726, 913, 971, 1120
Esp3I CGTCTC 3 cut(s) 150, 509, 678
FaeI CATG 8 cut(s) 161, 539, 609, 828, 933, 975, 1115, 1162
FalI AAGNNNNNCTT 4 cut(s) 744, 776, 825, 857
FatI CATG 8 cut(s) 157, 535, 605, 824, 929, 971, 1111, 1158
FauI CCCGC 1 cut(s) 49
Fnu4HI GCNGC 5 cut(s) 109, 129, 138, 327, 687
FokI GGATG 4 cut(s) 535, 744, 979, 1096
FriOI GRGCYC 3 cut(s) 23, 41, 880
Fsp4HI GCNGC 5 cut(s) 109, 129, 138, 327, 687
FspBI CTAG 5 cut(s) 269, 914, 1016, 1043, 1088
GlaI GCGC 1 cut(s) 45
GluI GCNGC 5 cut(s) 109, 129, 138, 327, 687
HaeIII GGCC 2 cut(s) 878, 1119
HapII CCGG 2 cut(s) 105, 1116
HgaI GACGC 2 cut(s) 660, 1102
HhaI GCGC 1 cut(s) 46
Hin1I GRCGYC 1 cut(s) 671
Hin1II CATG 8 cut(s) 161, 539, 609, 828, 933, 975, 1115, 1162
Hin6I GCGC 1 cut(s) 44
HinP1I GCGC 1 cut(s) 44
HindIII AAGCTT 2 cut(s) 709, 834
HinfI GANTC 4 cut(s) 82, 162, 438, 599
HpaII CCGG 2 cut(s) 105, 1116
HphI GGTGA 2 cut(s) 22, 715
Hpy166II GTNNAC 2 cut(s) 910, 986
Hpy188I TCNGA 1 cut(s) 367
Hpy188III TCNNGA 6 cut(s) 79, 193, 514, 865, 945, 1073
Hpy8I GTNNAC 2 cut(s) 910, 986
HpyAV CCTTC 3 cut(s) 230, 851, 1146
HpyCH4III ACNGT 3 cut(s) 243, 964, 990
HpyCH4IV ACGT 1 cut(s) 518
HpyCH4V TGCA 8 cut(s) 315, 656, 686, 788, 857, 910, 1025, 1104
HpyF10VI GCNNNNNNNGC 5 cut(s) 117, 134, 137, 562, 1093
HpyF3I CTNAG 4 cut(s) 226, 566, 712, 998
HpySE526I ACGT 1 cut(s) 518
Hsp92I GRCGYC 1 cut(s) 671
Hsp92II CATG 8 cut(s) 161, 539, 609, 828, 933, 975, 1115, 1162
HspAI GCGC 1 cut(s) 44
KroI GCCGGC 1 cut(s) 1115
KroNI GCCGGC 1 cut(s) 1117
Kzo9I GATC 1 cut(s) 941
LguI GCTCTTC 1 cut(s) 534
LmnI GCTCC 2 cut(s) 26, 145
Lsp1109I GCAGC 3 cut(s) 120, 124, 698
LweI GCATC 2 cut(s) 785, 1074
MaeI CTAG 5 cut(s) 269, 914, 1016, 1043, 1088
MaeII ACGT 1 cut(s) 518
MaeIII GTNAC 1 cut(s) 1090
MalI GATC 1 cut(s) 943
MboI GATC 1 cut(s) 941
MhlI GDGCHC 6 cut(s) 23, 41, 552, 832, 880, 912
MluCI AATT 1 cut(s) 634
MlyI GAGTC 2 cut(s) 171, 608
MnlI CCTC 7 cut(s) 83, 112, 133, 189, 235, 425, 456
Mph1103I ATGCAT 1 cut(s) 1027
MroNI GCCGGC 1 cut(s) 1115
MseI TTAA 4 cut(s) 284, 371, 584, 762
MslI CAYNNNNRTG 1 cut(s) 453
MspCI CTTAAG 1 cut(s) 761
MspI CCGG 2 cut(s) 105, 1116
MspR9I CCNGG 1 cut(s) 623
Mva1269I GAATGC 2 cut(s) 954, 1025
MvaI CCWGG 1 cut(s) 623
MvnI CGCG 2 cut(s) 44, 131
MwoI GCNNNNNNNGC 5 cut(s) 117, 134, 137, 562, 1093
NaeI GCCGGC 1 cut(s) 1117
NcoI CCATGG 2 cut(s) 157, 971
NdeII GATC 1 cut(s) 941
NgoMIV GCCGGC 1 cut(s) 1115
NheI GCTAGC 1 cut(s) 268
NlaIII CATG 8 cut(s) 161, 539, 609, 828, 933, 975, 1115, 1162
NlaIV GGNNCC 4 cut(s) 33, 191, 878, 879
NmuCI GTSAC 1 cut(s) 1090
NsiI ATGCAT 1 cut(s) 1027
NspI RCATGY 1 cut(s) 609
PciSI GCTCTTC 1 cut(s) 534
PctI GAATGC 2 cut(s) 954, 1025
PdiI GCCGGC 1 cut(s) 1117
PfeI GAWTC 2 cut(s) 82, 438
PflMI CCANNNNNTGG 1 cut(s) 732
PkrI GCNGC 5 cut(s) 110, 130, 139, 328, 688
PleI GAGTC 2 cut(s) 170, 607
PpsI GAGTC 2 cut(s) 170, 607
PshAI GACNNNNGTC 1 cut(s) 244
Psp124BI GAGCTC 1 cut(s) 23
Psp6I CCWGG 1 cut(s) 621
PspGI CCWGG 1 cut(s) 621
PspN4I GGNNCC 4 cut(s) 33, 191, 878, 879
PspOMI GGGCCC 1 cut(s) 876
PspPI GGNCC 3 cut(s) 377, 876, 877
PstI CTGCAG 1 cut(s) 688
RsaI GTAC 2 cut(s) 360, 1035
RsaNI GTAC 2 cut(s) 359, 1034
RseI CAYNNNNRTG 1 cut(s) 453
SacI GAGCTC 1 cut(s) 23
SapI GCTCTTC 1 cut(s) 534
SaqAI TTAA 4 cut(s) 284, 371, 584, 762
SatI GCNGC 5 cut(s) 109, 129, 138, 327, 687
Sau3AI GATC 1 cut(s) 941
Sau96I GGNCC 3 cut(s) 377, 876, 877
ScaI AGTACT 1 cut(s) 360
SchI GAGTC 2 cut(s) 171, 608
ScrFI CCNGG 1 cut(s) 623
SduI GDGCHC 6 cut(s) 23, 41, 552, 832, 880, 912
SfaNI GCATC 2 cut(s) 785, 1074
SfcI CTRYAG 1 cut(s) 684
SinI GGWCC 1 cut(s) 377
SmiMI CAYNNNNRTG 1 cut(s) 453
SmlI CTYRAG 1 cut(s) 761
SmoI CTYRAG 1 cut(s) 761
Sse9I AATT 1 cut(s) 634
SsiI CCGC 6 cut(s) 5, 42, 126, 129, 174, 326
SspMI CTAG 5 cut(s) 269, 914, 1016, 1043, 1088
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 1 cut(s) 621
StyI CCWWGG 5 cut(s) 157, 726, 913, 971, 1120
TaaI ACNGT 3 cut(s) 243, 964, 990
TaiI ACGT 1 cut(s) 521
TaqI TCGA 6 cut(s) 80, 153, 332, 452, 513, 597
TasI AATT 1 cut(s) 634
TatI WGTACW 2 cut(s) 358, 1033
TauI GCSGC 2 cut(s) 131, 329
TfiI GAWTC 2 cut(s) 82, 438
Tru1I TTAA 4 cut(s) 284, 371, 584, 762
Tru9I TTAA 4 cut(s) 284, 371, 584, 762
TscAI CASTG 1 cut(s) 688
TseFI GTSAC 1 cut(s) 1090
TseI GCWGC 3 cut(s) 108, 137, 686
Tsp45I GTSAC 1 cut(s) 1090
TspDTI ATGAA 4 cut(s) 46, 492, 552, 1100
TspGWI ACGGA 4 cut(s) 13, 132, 294, 773
TspRI CASTG 1 cut(s) 688
Van91I CCANNNNNTGG 1 cut(s) 732
Vha464I CTTAAG 1 cut(s) 761
VneI GTGCAC 1 cut(s) 908
VpaK11BI GGWCC 1 cut(s) 377
XceI RCATGY 1 cut(s) 609
XmaJI CCTAGG 1 cut(s) 913
XspI CTAG 5 cut(s) 269, 914, 1016, 1043, 1088
ZrmI AGTACT 1 cut(s) 360
Zsp2I ATGCAT 1 cut(s) 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.