Prupe.6G224200_v2.0.a1
ERF Family

Major facilitator superfamily domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp06
OG0007474
Physical Location & Seq
Forward (+)
22905820 .. 22910010
4191 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.6G224200.3

Sequence Viewer

Length: 1392 bp
ATGGGGGTGGTGATTGAAAGCTCTGTTTGGGAGCCAAATCCTGGCCTTTACATATTCATCTTCTTCGCCTCTCTCTTCTCCATCCTCGCCTTCCCTTATGCTTCAAACAAGTACAGCAGCACTGGCAGCAGCAGCAGCAGCTCCAGCAAAGCTCCGTCGCTCTTCGACCATGGAATCTCCCCCACCGCCTCCTCTCTCCGCTTCCAGAGGAACTTCCTTCTTATCTATTCTCTTGCCTCAGTGATGGAGGGACTGTGGTCTGTATTTGGTGAGTTTGAGTTAGCTTACTACGGTTTAAGCAGAGAGCAAATGATGTTTTCTCTCTGTGTTGGATTTGCAGCTTCTCTTTTAATTGGTTCCTTCTTAGGCGTACTTTCCGATGTAATAGGTCCAAAGAAAGTTTGTTTCCTCTTTTGCATTCTACACCTCTTTCTTGGCTTGTGGAAGAGAATTATTGATCATCCGAGCGTTTTGGCGACGAGTATTTGTTTGTCTCTCGCAGCTTCGATTTTTTCTTTCAGTTTCGAGACGTGGATGGTAGTTCAACATGAAGAGCAAGGGCACAGGCAAGATATGTTGAGTGAGACTTTTTGGTTGATGAGTTTCTTTGAGTCCGCATCTCTCATTGGAAGCCAGGTGCTTTCAAATTGGCTCATTGGTAATAATGTGGACAAAAACATGGCATCTCATTCCACTGCAGCCATCTTCTTGGCAATAATTGCCTTGGTTTGTCTTCTCAGAGGATGGACAGAAACCCCACAGAAAGTAGCTCTTAAGGAGTATAGGGCGTCTTTCTCTAAATATGTTTTTGGTGATAAAAGAATATGGCTTTTGGTGTGGGCACAGGCATGCCTTCACTTCTCTGTAGCAGTCTTCTGGATACTATGGGCCCCTATTATAGTGGCTGATGGACGAGAAGTGCACCTAGGGTTGATATATCCATGTTTTTTGGGCTCAAGAATGCTTGGAAGCACAATATTTCCGTGGCTCATTAGTGGACTGTCATCCCTGAGAACTGAGGATTGCCTAGTATATGCATTCATTATAATGGGGCTTGTCTTGTCCATAACAGCGTATGATTATCAGGAAATTGGAGTATTAGTGACATTATTTTGCATATTTCATGCCGGTCTTGGCATGATTTTTCCTTCACTAGCCAGATTGAGGACAATGTATGTGCCTAATGCACTGCGTGGGGGGATGATAAGCCTTTCTCAAGCCCCAGCAAATGCAGCAATTCTGCTTTTTCTGGTGCAAGGAAAATACTACAATAACATTGGGAACTCAACAATCATAGCGTTTGCTGCCCTTGGACTATTCACAGCAGCTGGTTGCATGCATGTCTTGAAGCGATACGGGAAGCAACCATATCACAACTGGCGTAAACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_5

Protein Analysis

464

Amino Acids

51.51

Weight (kDa)

8.6

Isoelectric Point (pI)

42.6

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1046
AccB7I CCANNNNNTGG 1 cut(s) 41
AciI CCGC 3 cut(s) 186, 199, 615
AcyI GRCGYC 1 cut(s) 788
AdeI CACNNNGTG 1 cut(s) 1193
AfaI GTAC 2 cut(s) 113, 372
AfiI CCNNNNNNNGG 2 cut(s) 41, 1164
AflII CTTAAG 1 cut(s) 773
AgsI TTSAA 5 cut(s) 17, 105, 545, 645, 1348
AjiI CACGTC 1 cut(s) 531
AjnI CCWGG 2 cut(s) 40, 633
AluBI AGCT 8 cut(s) 21, 141, 152, 284, 341, 503, 770, 1328
AluI AGCT 8 cut(s) 21, 141, 152, 284, 341, 503, 770, 1328
Alw21I GWGCWC 1 cut(s) 924
Alw26I GTCTC 3 cut(s) 498, 521, 578
Alw44I GTGCAC 1 cut(s) 920
AlwNI CAGNNNCTG 1 cut(s) 1328
AoxI GGCC 2 cut(s) 43, 888
ApaI GGGCCC 1 cut(s) 892
ApaLI GTGCAC 1 cut(s) 920
AspA2I CCTAGG 1 cut(s) 925
AspS9I GGNCC 3 cut(s) 389, 888, 889
AsuHPI GGTGA 3 cut(s) 22, 281, 824
AvaII GGWCC 1 cut(s) 389
AvrII CCTAGG 1 cut(s) 925
BaeGI GKGCMC 4 cut(s) 564, 844, 892, 924
BanII GRGCYC 2 cut(s) 892, 956
BbsI GAAGAC 2 cut(s) 725, 865
Bbv12I GWGCWC 1 cut(s) 924
BccI CCATC 6 cut(s) 89, 238, 529, 710, 738, 902
BciT130I CCWGG 2 cut(s) 42, 635
BciVI GTATCC 1 cut(s) 873
BclI TGATCA 1 cut(s) 457
BcoDI GTCTC 3 cut(s) 498, 521, 578
BfaI CTAG 3 cut(s) 926, 1028, 1154
BfmI CTRYAG 2 cut(s) 696, 864
BfrI CTTAAG 1 cut(s) 773
BfuI GTATCC 1 cut(s) 873
BlnI CCTAGG 1 cut(s) 925
Bme1390I CCNGG 2 cut(s) 42, 635
Bme18I GGWCC 1 cut(s) 389
BmgBI CACGTC 1 cut(s) 531
BmgT120I GGNCC 3 cut(s) 389, 888, 889
BmiI GGNNCC 4 cut(s) 33, 358, 890, 891
BmrFI CCNGG 2 cut(s) 42, 635
BmsI GCATC 2 cut(s) 626, 692
BoxI GACNNNNGTC 1 cut(s) 256
BpiI GAAGAC 2 cut(s) 725, 865
BpmI CTGGAG 1 cut(s) 127
BpuEI CTTGAG 2 cut(s) 940, 1200
BsaHI GRCGYC 1 cut(s) 788
BsaJI CCNNGG 5 cut(s) 169, 723, 925, 983, 1309
BsaXI ACNNNNNCTCC 4 cut(s) 239, 269, 1086, 1116
Bsc4I CCNNNNNNNGG 2 cut(s) 41, 1164
Bse118I RCCGGY 1 cut(s) 1127
Bse1I ACTGG 2 cut(s) 127, 1382
BseBI CCWGG 2 cut(s) 42, 635
BseDI CCNNGG 5 cut(s) 169, 723, 925, 983, 1309
BseGI GGATG 6 cut(s) 81, 460, 540, 749, 1004, 1206
BseLI CCNNNNNNNGG 2 cut(s) 41, 1164
BseMII CTCAG 4 cut(s) 252, 751, 1001, 1008
BseNI ACTGG 2 cut(s) 127, 1382
BseRI GAGGAG 1 cut(s) 181
BseSI GKGCMC 4 cut(s) 564, 844, 892, 924
BseYI CCCAGC 1 cut(s) 1222
BshFI GGCC 2 cut(s) 45, 890
BsiHKAI GWGCWC 1 cut(s) 924
BsiSI CCGG 1 cut(s) 1128
BslFI GGGAC 1 cut(s) 264
BslI CCNNNNNNNGG 2 cut(s) 41, 1164
BsmAI GTCTC 3 cut(s) 498, 521, 578
BsmBI CGTCTC 1 cut(s) 521
BsmFI GGGAC 1 cut(s) 264
BsmI GAATGC 3 cut(s) 417, 966, 1037
BsnI GGCC 2 cut(s) 45, 890
Bsp120I GGGCCC 1 cut(s) 888
Bsp1286I GDGCHC 5 cut(s) 564, 844, 892, 924, 956
Bsp143I GATC 1 cut(s) 457
Bsp19I CCATGG 1 cut(s) 169
BspACI CCGC 3 cut(s) 186, 199, 615
BspANI GGCC 2 cut(s) 45, 890
BspCNI CTCAG 4 cut(s) 251, 750, 1002, 1009
BspLI GGNNCC 4 cut(s) 33, 358, 890, 891
BspMAI CTGCAG 1 cut(s) 700
BspQI GCTCTTC 2 cut(s) 167, 546
BspTI CTTAAG 1 cut(s) 773
BsrFI RCCGGY 1 cut(s) 1127
BsrI ACTGG 2 cut(s) 127, 1382
BssAI RCCGGY 1 cut(s) 1127
BssECI CCNNGG 5 cut(s) 169, 723, 925, 983, 1309
BssMI GATC 1 cut(s) 457
BssNI GRCGYC 1 cut(s) 788
BssT1I CCWWGG 4 cut(s) 169, 723, 925, 1309
Bst2UI CCWGG 2 cut(s) 42, 635
Bst4CI ACNGT 3 cut(s) 255, 293, 1002
Bst6I CTCTTC 4 cut(s) 80, 167, 440, 546
BstACI GRCGYC 1 cut(s) 788
BstAFI CTTAAG 1 cut(s) 773
BstAPI GCANNNNNTGC 1 cut(s) 719
BstC8I GCNNGC 2 cut(s) 850, 1337
BstDEI CTNAG 5 cut(s) 238, 364, 737, 1010, 1017
BstDSI CCRYGG 2 cut(s) 169, 983
BstF5I GGATG 6 cut(s) 81, 460, 540, 749, 1004, 1206
BstKTI GATC 1 cut(s) 460
BstMAI GTCTC 3 cut(s) 498, 521, 578
BstMBI GATC 1 cut(s) 457
BstMWI GCNNNNNNNGC 9 cut(s) 123, 126, 132, 135, 138, 144, 719, 1232, 1304
BstNI CCWGG 2 cut(s) 42, 635
BstNSI RCATGY 3 cut(s) 852, 1339, 1343
BstPAI GACNNNNGTC 1 cut(s) 256
BstSCI CCNGG 2 cut(s) 40, 633
BstSFI CTRYAG 2 cut(s) 696, 864
BstSLI GKGCMC 4 cut(s) 564, 844, 892, 924
BstV2I GAAGAC 2 cut(s) 725, 865
BstXI CCANNNNNNTGG 1 cut(s) 709
BsuI GTATCC 1 cut(s) 873
BsuRI GGCC 2 cut(s) 45, 890
BtgI CCRYGG 2 cut(s) 169, 983
BtrI CACGTC 1 cut(s) 531
BtsCI GGATG 6 cut(s) 81, 460, 540, 749, 1004, 1206
BtsI GCAGTG 2 cut(s) 693, 1187
BtsIMutI CAGTG 4 cut(s) 120, 246, 693, 1187
Cac8I GCNNGC 2 cut(s) 850, 1337
CaiI CAGNNNCTG 1 cut(s) 1328
Cfr10I RCCGGY 1 cut(s) 1127
Cfr13I GGNCC 3 cut(s) 389, 888, 889
CseI GACGC 1 cut(s) 777
Csp6I GTAC 2 cut(s) 112, 371
CviAII CATG 9 cut(s) 170, 548, 679, 849, 942, 1124, 1138, 1336, 1340
CviQI GTAC 2 cut(s) 112, 371
DdeI CTNAG 5 cut(s) 238, 364, 737, 1010, 1017
DpnI GATC 1 cut(s) 459
DpnII GATC 1 cut(s) 457
DraIII CACNNNGTG 1 cut(s) 1193
Eam1104I CTCTTC 4 cut(s) 80, 167, 440, 546
EarI CTCTTC 4 cut(s) 80, 167, 440, 546
Eco130I CCWWGG 4 cut(s) 169, 723, 925, 1309
Eco24I GRGCYC 2 cut(s) 892, 956
Eco47I GGWCC 1 cut(s) 389
EcoO109I RGGNCCY 1 cut(s) 889
EcoRII CCWGG 2 cut(s) 40, 633
EcoT14I CCWWGG 4 cut(s) 169, 723, 925, 1309
EcoT22I ATGCAT 2 cut(s) 1039, 1341
EcoT38I GRGCYC 2 cut(s) 892, 956
ErhI CCWWGG 4 cut(s) 169, 723, 925, 1309
Esp3I CGTCTC 1 cut(s) 521
FaeI CATG 9 cut(s) 173, 551, 682, 852, 945, 1127, 1141, 1339, 1343
FalI AAGNNNNNCTT 2 cut(s) 756, 788
FaqI GGGAC 1 cut(s) 264
FatI CATG 9 cut(s) 169, 547, 678, 848, 941, 1123, 1137, 1335, 1339
FbaI TGATCA 1 cut(s) 457
FokI GGATG 6 cut(s) 68, 447, 547, 756, 991, 1213
FriOI GRGCYC 2 cut(s) 892, 956
FspBI CTAG 3 cut(s) 926, 1028, 1154
GsaI CCCAGC 1 cut(s) 1226
GsuI CTGGAG 1 cut(s) 127
HaeIII GGCC 2 cut(s) 45, 890
HapII CCGG 1 cut(s) 1128
HgaI GACGC 1 cut(s) 777
Hin1I GRCGYC 1 cut(s) 788
Hin1II CATG 9 cut(s) 173, 551, 682, 852, 945, 1127, 1141, 1339, 1343
HinfI GANTC 2 cut(s) 174, 611
HpaII CCGG 1 cut(s) 1128
HphI GGTGA 3 cut(s) 22, 281, 824
Hpy166II GTNNAC 4 cut(s) 670, 922, 998, 1385
Hpy188I TCNGA 4 cut(s) 379, 465, 740, 1391
Hpy188III TCNNGA 6 cut(s) 205, 526, 877, 957, 1085, 1345
Hpy8I GTNNAC 4 cut(s) 670, 922, 998, 1385
Hpy99I CGWCG 2 cut(s) 160, 481
HpyAV CCTTC 5 cut(s) 100, 227, 370, 863, 1158
HpyCH4III ACNGT 3 cut(s) 255, 293, 1002
HpyCH4IV ACGT 1 cut(s) 530
HpyF10VI GCNNNNNNNGC 9 cut(s) 123, 126, 132, 135, 138, 144, 719, 1232, 1304
HpyF3I CTNAG 5 cut(s) 238, 364, 737, 1010, 1017
HpySE526I ACGT 1 cut(s) 530
Hsp92I GRCGYC 1 cut(s) 788
Hsp92II CATG 9 cut(s) 173, 551, 682, 852, 945, 1127, 1141, 1339, 1343
Ksp22I TGATCA 1 cut(s) 457
Kzo9I GATC 1 cut(s) 457
LguI GCTCTTC 2 cut(s) 167, 546
LmnI GCTCC 3 cut(s) 31, 146, 157
LweI GCATC 2 cut(s) 626, 692
MaeI CTAG 3 cut(s) 926, 1028, 1154
MaeII ACGT 1 cut(s) 530
MaeIII GTNAC 1 cut(s) 1102
MalI GATC 1 cut(s) 459
MboI GATC 1 cut(s) 457
MboII GAAGA 9 cut(s) 52, 55, 67, 154, 457, 563, 697, 725, 865
MhlI GDGCHC 5 cut(s) 564, 844, 892, 924, 956
MluCI AATT 6 cut(s) 351, 450, 646, 717, 1089, 1236
MlyI GAGTC 1 cut(s) 620
MmeI TCCRAC 1 cut(s) 310
Mph1103I ATGCAT 2 cut(s) 1039, 1341
MseI TTAA 3 cut(s) 296, 350, 774
MslI CAYNNNNRTG 2 cut(s) 847, 1046
MspA1I CMGCKG 1 cut(s) 1328
MspCI CTTAAG 1 cut(s) 773
MspI CCGG 1 cut(s) 1128
MspR9I CCNGG 2 cut(s) 42, 635
Mva1269I GAATGC 3 cut(s) 417, 966, 1037
MvaI CCWGG 2 cut(s) 42, 635
MwoI GCNNNNNNNGC 9 cut(s) 123, 126, 132, 135, 138, 144, 719, 1232, 1304
NcoI CCATGG 1 cut(s) 169
NdeII GATC 1 cut(s) 457
NlaIII CATG 9 cut(s) 173, 551, 682, 852, 945, 1127, 1141, 1339, 1343
NlaIV GGNNCC 4 cut(s) 33, 358, 890, 891
NmuCI GTSAC 1 cut(s) 1102
NsiI ATGCAT 2 cut(s) 1039, 1341
NspI RCATGY 3 cut(s) 852, 1339, 1343
PaeI GCATGC 2 cut(s) 852, 1339
PciSI GCTCTTC 2 cut(s) 167, 546
PctI GAATGC 3 cut(s) 417, 966, 1037
PfeI GAWTC 1 cut(s) 174
PflMI CCANNNNNTGG 1 cut(s) 41
PleI GAGTC 1 cut(s) 619
PpsI GAGTC 1 cut(s) 619
PshAI GACNNNNGTC 1 cut(s) 256
PsiI TTATAA 1 cut(s) 1046
Psp6I CCWGG 2 cut(s) 40, 633
PspFI CCCAGC 1 cut(s) 1222
PspGI CCWGG 2 cut(s) 40, 633
PspN4I GGNNCC 4 cut(s) 33, 358, 890, 891
PspOMI GGGCCC 1 cut(s) 888
PspPI GGNCC 3 cut(s) 389, 888, 889
PstI CTGCAG 1 cut(s) 700
PstNI CAGNNNCTG 1 cut(s) 1328
PvuII CAGCTG 1 cut(s) 1328
RsaI GTAC 2 cut(s) 113, 372
RsaNI GTAC 2 cut(s) 112, 371
RseI CAYNNNNRTG 2 cut(s) 847, 1046
SapI GCTCTTC 2 cut(s) 167, 546
SaqAI TTAA 3 cut(s) 296, 350, 774
Sau3AI GATC 1 cut(s) 457
Sau96I GGNCC 3 cut(s) 389, 888, 889
SchI GAGTC 1 cut(s) 620
ScrFI CCNGG 2 cut(s) 42, 635
SduI GDGCHC 5 cut(s) 564, 844, 892, 924, 956
SfaNI GCATC 2 cut(s) 626, 692
SfcI CTRYAG 2 cut(s) 696, 864
SinI GGWCC 1 cut(s) 389
SmiMI CAYNNNNRTG 2 cut(s) 847, 1046
SmlI CTYRAG 3 cut(s) 773, 955, 1215
SmoI CTYRAG 3 cut(s) 773, 955, 1215
SphI GCATGC 2 cut(s) 852, 1339
Sse9I AATT 6 cut(s) 351, 450, 646, 717, 1089, 1236
SsiI CCGC 3 cut(s) 186, 199, 615
SspI AATATT 1 cut(s) 978
SspMI CTAG 3 cut(s) 926, 1028, 1154
StyD4I CCNGG 2 cut(s) 40, 633
StyI CCWWGG 4 cut(s) 169, 723, 925, 1309
TaaI ACNGT 3 cut(s) 255, 293, 1002
TaiI ACGT 1 cut(s) 533
TaqI TCGA 3 cut(s) 165, 506, 525
TasI AATT 6 cut(s) 351, 450, 646, 717, 1089, 1236
TatI WGTACW 1 cut(s) 111
TfiI GAWTC 1 cut(s) 174
Tru1I TTAA 3 cut(s) 296, 350, 774
Tru9I TTAA 3 cut(s) 296, 350, 774
TscAI CASTG 4 cut(s) 127, 246, 700, 1194
TseFI GTSAC 1 cut(s) 1102
Tsp45I GTSAC 1 cut(s) 1102
TspDTI ATGAA 4 cut(s) 46, 564, 1030, 1112
TspGWI ACGGA 2 cut(s) 144, 972
TspRI CASTG 4 cut(s) 127, 246, 700, 1194
Van91I CCANNNNNTGG 1 cut(s) 41
Vha464I CTTAAG 1 cut(s) 773
VneI GTGCAC 1 cut(s) 920
VpaK11BI GGWCC 1 cut(s) 389
XceI RCATGY 3 cut(s) 852, 1339, 1343
XcmI CCANNNNNNNNNTGG 1 cut(s) 1374
XmaJI CCTAGG 1 cut(s) 925
XspI CTAG 3 cut(s) 926, 1028, 1154
Zsp2I ATGCAT 2 cut(s) 1039, 1341
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.