Rroxscaffold_2G00136390
ERF Family

Major facilitator superfamily domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0007474
Physical Location & Seq
Forward (+)
74107264 .. 74111119
3856 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00136390.1

Sequence Viewer

Length: 1380 bp
ATGGCGGTGGTGATTGAGAGCTCCGTTTGGGAACCGAGCCCCGCGCTTTACATCTTCATCTTCTTCGCCTGCCTCTTCTCGATTCTCTTCTTCCCTTACGCCTCCGGCAGCACCAACAGCACCTCCGCCGCGAAAGCTGCTCCGTCTCTGTTCGACCATGGAGTCTCCCCCACCGCTTCGTCTTTTCGGTTCCAGAGGAACTTCCTGCTTCTCTATTCTCTCGCCTCAGTTATGGAAGGACTGTGGTCGGTGTTCGGTGAAGTTGAGCTAGCTTACTACGGATTAAGCAAAGAGAAAATGGTGCTTTATCTCTGTGTAGGACTTGCGGCTTCGATTTCTATCGGTTCTTTCTTAGGAGTACTTTCTGATTTAATAGGTCCAAAGAAAGTTTGCTTCTTCTTTTGCTTTCTACACCTCCTTGTTGGCTTGTTGAAGAGAATCGTCCCTCATTTGAGTGTTTGGGTGGCGAGTGTTTGTTTGTCAATCACAAGTTCAATTTTTTCGTTCGGTTTTGAGACGTGGATGGTACTTCAACATGAAGAGCAAGGGCACAGGCAAGATATGCTGAGTGAGACATTTTGGTTAATGAGTTTCTTCGAGTCAGCATGTCTTATTGGTAGCCAGGTTTTTTCAAATTGGCTCATCGGTAATAATGCAGAGAGAAGTATGGCGTCTCATTCTACTGCAGCTATCTTCTTGGCAGTAATAAGCTTAGTCTGTCTCACCAAGGGATGGAAAGAAATCCCACATAAAGTAGCTCTTAAGGAGTATAGGGCATCTTTTCGTGCATACATTGTTGGCGATAAAAGAGTATGGCTTTTGGCATGGGCACAAGCTTGCCTTCACTTCTCTGTAGCAGTATTTTGGATACTATGGGCCCCAACAATAGTGGCTGATGGACGAGAAGTGCACCTAGGGTTGATATATCCGTGTTTTCTTGGATCAAGAATGCTTGGAAGCACAGTATTCCCATGGCTCATTAGTGGACTGTCATCCCTTAGAACTGATGATTGCCTAGTATATGCATTCATTGTACTGGGGCTAGTAATGTCCATAACAGCTTATGATTATCAGGAAGTTGGGATGCTAGTGACACTATTTTGCATATTTCATGCCGGCCTTGGCGTGATTTGGCCTTCACTTGCCAGATTGAGAACCATGTATGTGCCTAATGCACTGCGTGGAGGGATGATAAGCCTTTCTCAAGCCCCTGCAAATGCAGCAATTCTGCTTTTTCTGGTGCAAGGAAAGTACCACAATAACATTGGAAACTCGACGATCATAGCATTTGCTGCCCTTGGGTTATTCACAGCAGCCGGTTGCATGCATGTCTTGAAGCGATGGGGGAAGCAACCATATCAGAACTGGCACAAATTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MFS_5

Protein Analysis

459

Amino Acids

50.95

Weight (kDa)

8.66

Isoelectric Point (pI)

45.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MFS_5 PF05631 63 - 351 1.7e-26 Sugar-tranasporters, 12 TM
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 161
AccB7I CCANNNNNTGG 1 cut(s) 732
AccII CGCG 2 cut(s) 44, 131
AciI CCGC 6 cut(s) 5, 42, 126, 129, 174, 326
AclWI GGATC 1 cut(s) 949
AcyI GRCGYC 1 cut(s) 671
AdeI CACNNNGTG 1 cut(s) 1181
AfaI GTAC 4 cut(s) 360, 528, 1035, 1253
AfiI CCNNNNNNNGG 1 cut(s) 732
AflII CTTAAG 1 cut(s) 761
AgsI TTSAA 5 cut(s) 433, 495, 533, 633, 1336
AjiI CACGTC 1 cut(s) 519
AjnI CCWGG 1 cut(s) 621
AluBI AGCT 9 cut(s) 21, 137, 268, 272, 689, 711, 758, 836, 1061
AluI AGCT 9 cut(s) 21, 137, 268, 272, 689, 711, 758, 836, 1061
Alw21I GWGCWC 2 cut(s) 23, 912
Alw26I GTCTC 6 cut(s) 150, 169, 509, 566, 678, 725
Alw44I GTGCAC 1 cut(s) 908
AlwI GGATC 1 cut(s) 949
AoxI GGCC 3 cut(s) 876, 1117, 1133
ApaI GGGCCC 1 cut(s) 880
ApaLI GTGCAC 1 cut(s) 908
ApeKI GCWGC 6 cut(s) 108, 137, 686, 1220, 1292, 1313
AspA2I CCTAGG 1 cut(s) 913
AspLEI GCGC 1 cut(s) 46
AspS9I GGNCC 3 cut(s) 377, 876, 877
AsuHPI GGTGA 3 cut(s) 22, 269, 715
AsuNHI GCTAGC 1 cut(s) 268
AvaII GGWCC 1 cut(s) 377
AvrII CCTAGG 1 cut(s) 913
BaeGI GKGCMC 4 cut(s) 552, 832, 880, 912
BanII GRGCYC 3 cut(s) 23, 41, 880
Bbv12I GWGCWC 2 cut(s) 23, 912
BbvI GCAGC 6 cut(s) 120, 124, 698, 1232, 1279, 1325
BccI CCATC 4 cut(s) 517, 726, 890, 1335
BciT130I CCWGG 1 cut(s) 623
BciVI GTATCC 1 cut(s) 861
BcoDI GTCTC 6 cut(s) 150, 169, 509, 566, 678, 725
BfaI CTAG 5 cut(s) 269, 914, 1016, 1043, 1088
BfmI CTRYAG 2 cut(s) 684, 852
BfrI CTTAAG 1 cut(s) 761
BfuI GTATCC 1 cut(s) 861
BisI GCNGC 8 cut(s) 109, 129, 138, 327, 687, 1221, 1293, 1314
BlnI CCTAGG 1 cut(s) 913
BlsI GCNGC 8 cut(s) 110, 130, 139, 328, 688, 1222, 1294, 1315
BmcAI AGTACT 1 cut(s) 360
Bme1390I CCNGG 1 cut(s) 623
Bme18I GGWCC 1 cut(s) 377
BmgBI CACGTC 1 cut(s) 519
BmgT120I GGNCC 3 cut(s) 377, 876, 877
BmiI GGNNCC 4 cut(s) 33, 191, 878, 879
BmrFI CCNGG 1 cut(s) 623
BmrI ACTGGG 1 cut(s) 1046
BmsI GCATC 2 cut(s) 785, 1074
BmtI GCTAGC 1 cut(s) 272
BmuI ACTGGG 1 cut(s) 1046
BoxI GACNNNNGTC 1 cut(s) 244
BpuEI CTTGAG 1 cut(s) 1188
BsaBI GATNNNNATC 1 cut(s) 338
BsaHI GRCGYC 1 cut(s) 671
BsaJI CCNNGG 6 cut(s) 157, 726, 913, 971, 1120, 1297
BsaXI ACNNNNNCTCC 2 cut(s) 107, 137
Bsc4I CCNNNNNNNGG 1 cut(s) 732
Bse118I RCCGGY 2 cut(s) 1115, 1316
Bse1I ACTGG 2 cut(s) 1041, 1370
Bse8I GATNNNNATC 1 cut(s) 338
BseBI CCWGG 1 cut(s) 623
BseDI CCNNGG 6 cut(s) 157, 726, 913, 971, 1120, 1297
BseGI GGATG 5 cut(s) 528, 737, 992, 1089, 1194
BseJI GATNNNNATC 1 cut(s) 338
BseLI CCNNNNNNNGG 1 cut(s) 732
BseMII CTCAG 2 cut(s) 240, 557
BseNI ACTGG 2 cut(s) 1041, 1370
BseSI GKGCMC 4 cut(s) 552, 832, 880, 912
BseXI GCAGC 6 cut(s) 120, 124, 698, 1232, 1279, 1325
Bsh1236I CGCG 2 cut(s) 44, 131
BshFI GGCC 3 cut(s) 878, 1119, 1135
BsiHKAI GWGCWC 2 cut(s) 23, 912
BsiSI CCGG 3 cut(s) 105, 1116, 1317
BslFI GGGAC 1 cut(s) 428
BslI CCNNNNNNNGG 1 cut(s) 732
BsmAI GTCTC 6 cut(s) 150, 169, 509, 566, 678, 725
BsmBI CGTCTC 3 cut(s) 150, 509, 678
BsmFI GGGAC 1 cut(s) 428
BsmI GAATGC 2 cut(s) 954, 1025
BsnI GGCC 3 cut(s) 878, 1119, 1135
Bsp120I GGGCCC 1 cut(s) 876
Bsp1286I GDGCHC 6 cut(s) 23, 41, 552, 832, 880, 912
Bsp143I GATC 2 cut(s) 941, 1278
Bsp19I CCATGG 2 cut(s) 157, 971
BspACI CCGC 6 cut(s) 5, 42, 126, 129, 174, 326
BspANI GGCC 3 cut(s) 878, 1119, 1135
BspCNI CTCAG 2 cut(s) 239, 558
BspFNI CGCG 2 cut(s) 44, 131
BspLI GGNNCC 4 cut(s) 33, 191, 878, 879
BspMAI CTGCAG 1 cut(s) 688
BspOI GCTAGC 1 cut(s) 272
BspPI GGATC 1 cut(s) 949
BspQI GCTCTTC 1 cut(s) 534
BspTI CTTAAG 1 cut(s) 761
BsrFI RCCGGY 2 cut(s) 1115, 1316
BsrI ACTGG 2 cut(s) 1041, 1370
BssAI RCCGGY 2 cut(s) 1115, 1316
BssECI CCNNGG 6 cut(s) 157, 726, 913, 971, 1120, 1297
BssMI GATC 2 cut(s) 941, 1278
BssNI GRCGYC 1 cut(s) 671
BssT1I CCWWGG 6 cut(s) 157, 726, 913, 971, 1120, 1297
Bst2UI CCWGG 1 cut(s) 623
Bst4CI ACNGT 3 cut(s) 243, 964, 990
Bst6I CTCTTC 4 cut(s) 80, 92, 428, 534
BstACI GRCGYC 1 cut(s) 671
BstAFI CTTAAG 1 cut(s) 761
BstAPI GCANNNNNTGC 2 cut(s) 562, 1292
BstC8I GCNNGC 5 cut(s) 70, 270, 838, 1117, 1325
BstDEI CTNAG 5 cut(s) 226, 352, 566, 712, 998
BstDSI CCRYGG 2 cut(s) 157, 971
BstF5I GGATG 5 cut(s) 528, 737, 992, 1089, 1194
BstFNI CGCG 2 cut(s) 44, 131
BstHHI GCGC 1 cut(s) 46
BstKTI GATC 2 cut(s) 944, 1281
BstMAI GTCTC 6 cut(s) 150, 169, 509, 566, 678, 725
BstMBI GATC 2 cut(s) 941, 1278
BstMWI GCNNNNNNNGC 6 cut(s) 117, 134, 137, 562, 1220, 1292
BstNI CCWGG 1 cut(s) 623
BstNSI RCATGY 3 cut(s) 609, 1327, 1331
BstPAI GACNNNNGTC 1 cut(s) 244
BstSCI CCNGG 1 cut(s) 621
BstSFI CTRYAG 2 cut(s) 684, 852
BstSLI GKGCMC 4 cut(s) 552, 832, 880, 912
BstUI CGCG 2 cut(s) 44, 131
BstV1I GCAGC 6 cut(s) 120, 124, 698, 1232, 1279, 1325
BsuI GTATCC 1 cut(s) 861
BsuRI GGCC 3 cut(s) 878, 1119, 1135
BtgI CCRYGG 2 cut(s) 157, 971
BtgZI GCGATG 1 cut(s) 1354
BtrI CACGTC 1 cut(s) 519
BtsCI GGATG 5 cut(s) 528, 737, 992, 1089, 1194
BtsI GCAGTG 1 cut(s) 1175
BtsIMutI CAGTG 1 cut(s) 1175
Cac8I GCNNGC 5 cut(s) 70, 270, 838, 1117, 1325
CfoI GCGC 1 cut(s) 46
Cfr10I RCCGGY 2 cut(s) 1115, 1316
Cfr13I GGNCC 3 cut(s) 377, 876, 877
CseI GACGC 1 cut(s) 660
Csp6I GTAC 4 cut(s) 359, 527, 1034, 1252
CspCI CAANNNNNGTGG 2 cut(s) 870, 905
CviAII CATG 9 cut(s) 158, 536, 606, 825, 972, 1112, 1159, 1324, 1328
CviQI GTAC 4 cut(s) 359, 527, 1034, 1252
DdeI CTNAG 5 cut(s) 226, 352, 566, 712, 998
DpnI GATC 2 cut(s) 943, 1280
DpnII GATC 2 cut(s) 941, 1278
DraIII CACNNNGTG 1 cut(s) 1181
DrdI GACNNNNNNGTC 1 cut(s) 161
DseDI GACNNNNNNGTC 1 cut(s) 161
Eam1104I CTCTTC 4 cut(s) 80, 92, 428, 534
EarI CTCTTC 4 cut(s) 80, 92, 428, 534
EciI GGCGGA 1 cut(s) 115
Ecl136II GAGCTC 1 cut(s) 21
Eco130I CCWWGG 6 cut(s) 157, 726, 913, 971, 1120, 1297
Eco24I GRGCYC 3 cut(s) 23, 41, 880
Eco47I GGWCC 1 cut(s) 377
Eco53kI GAGCTC 1 cut(s) 21
EcoICRI GAGCTC 1 cut(s) 21
EcoO109I RGGNCCY 1 cut(s) 877
EcoRII CCWGG 1 cut(s) 621
EcoT14I CCWWGG 6 cut(s) 157, 726, 913, 971, 1120, 1297
EcoT22I ATGCAT 2 cut(s) 1027, 1329
EcoT38I GRGCYC 3 cut(s) 23, 41, 880
ErhI CCWWGG 6 cut(s) 157, 726, 913, 971, 1120, 1297
Esp3I CGTCTC 3 cut(s) 150, 509, 678
FaeI CATG 9 cut(s) 161, 539, 609, 828, 975, 1115, 1162, 1327, 1331
FalI AAGNNNNNCTT 4 cut(s) 744, 776, 825, 857
FaqI GGGAC 1 cut(s) 428
FatI CATG 9 cut(s) 157, 535, 605, 824, 971, 1111, 1158, 1323, 1327
FauI CCCGC 1 cut(s) 49
Fnu4HI GCNGC 8 cut(s) 109, 129, 138, 327, 687, 1221, 1293, 1314
FokI GGATG 5 cut(s) 535, 744, 979, 1096, 1201
FriOI GRGCYC 3 cut(s) 23, 41, 880
Fsp4HI GCNGC 8 cut(s) 109, 129, 138, 327, 687, 1221, 1293, 1314
FspBI CTAG 5 cut(s) 269, 914, 1016, 1043, 1088
GlaI GCGC 1 cut(s) 45
GluI GCNGC 8 cut(s) 109, 129, 138, 327, 687, 1221, 1293, 1314
HaeIII GGCC 3 cut(s) 878, 1119, 1135
HapII CCGG 3 cut(s) 105, 1116, 1317
HgaI GACGC 1 cut(s) 660
HhaI GCGC 1 cut(s) 46
Hin1I GRCGYC 1 cut(s) 671
Hin1II CATG 9 cut(s) 161, 539, 609, 828, 975, 1115, 1162, 1327, 1331
Hin6I GCGC 1 cut(s) 44
HinP1I GCGC 1 cut(s) 44
HindIII AAGCTT 2 cut(s) 709, 834
HinfI GANTC 4 cut(s) 82, 162, 438, 599
HpaII CCGG 3 cut(s) 105, 1116, 1317
HphI GGTGA 3 cut(s) 22, 269, 715
Hpy166II GTNNAC 2 cut(s) 910, 986
Hpy188I TCNGA 2 cut(s) 367, 1362
Hpy188III TCNNGA 5 cut(s) 79, 193, 945, 1073, 1333
Hpy8I GTNNAC 2 cut(s) 910, 986
Hpy99I CGWCG 1 cut(s) 1279
HpyAV CCTTC 3 cut(s) 230, 851, 1146
HpyCH4III ACNGT 3 cut(s) 243, 964, 990
HpyCH4IV ACGT 1 cut(s) 518
HpyF10VI GCNNNNNNNGC 6 cut(s) 117, 134, 137, 562, 1220, 1292
HpyF3I CTNAG 5 cut(s) 226, 352, 566, 712, 998
HpySE526I ACGT 1 cut(s) 518
Hsp92I GRCGYC 1 cut(s) 671
Hsp92II CATG 9 cut(s) 161, 539, 609, 828, 975, 1115, 1162, 1327, 1331
HspAI GCGC 1 cut(s) 44
KroI GCCGGC 1 cut(s) 1115
KroNI GCCGGC 1 cut(s) 1117
Kzo9I GATC 2 cut(s) 941, 1278
LguI GCTCTTC 1 cut(s) 534
LmnI GCTCC 2 cut(s) 26, 145
Lsp1109I GCAGC 6 cut(s) 120, 124, 698, 1232, 1279, 1325
LweI GCATC 2 cut(s) 785, 1074
MaeI CTAG 5 cut(s) 269, 914, 1016, 1043, 1088
MaeII ACGT 1 cut(s) 518
MaeIII GTNAC 1 cut(s) 1090
MalI GATC 2 cut(s) 943, 1280
MboI GATC 2 cut(s) 941, 1278
MhlI GDGCHC 6 cut(s) 23, 41, 552, 832, 880, 912
MluCI AATT 4 cut(s) 495, 634, 1224, 1373
MlyI GAGTC 2 cut(s) 171, 608
MnlI CCTC 8 cut(s) 83, 112, 133, 189, 235, 425, 456, 1178
Mph1103I ATGCAT 2 cut(s) 1027, 1329
MroNI GCCGGC 1 cut(s) 1115
MseI TTAA 4 cut(s) 284, 371, 584, 762
MslI CAYNNNNRTG 2 cut(s) 453, 1163
MspCI CTTAAG 1 cut(s) 761
MspI CCGG 3 cut(s) 105, 1116, 1317
MspR9I CCNGG 1 cut(s) 623
Mva1269I GAATGC 2 cut(s) 954, 1025
MvaI CCWGG 1 cut(s) 623
MvnI CGCG 2 cut(s) 44, 131
MwoI GCNNNNNNNGC 6 cut(s) 117, 134, 137, 562, 1220, 1292
NaeI GCCGGC 1 cut(s) 1117
NcoI CCATGG 2 cut(s) 157, 971
NdeII GATC 2 cut(s) 941, 1278
NgoMIV GCCGGC 1 cut(s) 1115
NheI GCTAGC 1 cut(s) 268
NlaIII CATG 9 cut(s) 161, 539, 609, 828, 975, 1115, 1162, 1327, 1331
NlaIV GGNNCC 4 cut(s) 33, 191, 878, 879
NmuCI GTSAC 1 cut(s) 1090
NsiI ATGCAT 2 cut(s) 1027, 1329
NspI RCATGY 3 cut(s) 609, 1327, 1331
PaeI GCATGC 1 cut(s) 1327
PciSI GCTCTTC 1 cut(s) 534
PctI GAATGC 2 cut(s) 954, 1025
PdiI GCCGGC 1 cut(s) 1117
PfeI GAWTC 2 cut(s) 82, 438
PflMI CCANNNNNTGG 1 cut(s) 732
PkrI GCNGC 8 cut(s) 110, 130, 139, 328, 688, 1222, 1294, 1315
PleI GAGTC 2 cut(s) 170, 607
PpsI GAGTC 2 cut(s) 170, 607
PshAI GACNNNNGTC 1 cut(s) 244
Psp124BI GAGCTC 1 cut(s) 23
Psp6I CCWGG 1 cut(s) 621
PspGI CCWGG 1 cut(s) 621
PspN4I GGNNCC 4 cut(s) 33, 191, 878, 879
PspOMI GGGCCC 1 cut(s) 876
PspPI GGNCC 3 cut(s) 377, 876, 877
PstI CTGCAG 1 cut(s) 688
RsaI GTAC 4 cut(s) 360, 528, 1035, 1253
RsaNI GTAC 4 cut(s) 359, 527, 1034, 1252
RseI CAYNNNNRTG 2 cut(s) 453, 1163
SacI GAGCTC 1 cut(s) 23
SapI GCTCTTC 1 cut(s) 534
SaqAI TTAA 4 cut(s) 284, 371, 584, 762
SatI GCNGC 8 cut(s) 109, 129, 138, 327, 687, 1221, 1293, 1314
Sau3AI GATC 2 cut(s) 941, 1278
Sau96I GGNCC 3 cut(s) 377, 876, 877
ScaI AGTACT 1 cut(s) 360
SchI GAGTC 2 cut(s) 171, 608
ScrFI CCNGG 1 cut(s) 623
SduI GDGCHC 6 cut(s) 23, 41, 552, 832, 880, 912
SfaNI GCATC 2 cut(s) 785, 1074
SfcI CTRYAG 2 cut(s) 684, 852
SinI GGWCC 1 cut(s) 377
SmiMI CAYNNNNRTG 2 cut(s) 453, 1163
SmlI CTYRAG 2 cut(s) 761, 1203
SmoI CTYRAG 2 cut(s) 761, 1203
SphI GCATGC 1 cut(s) 1327
Sse9I AATT 4 cut(s) 495, 634, 1224, 1373
SsiI CCGC 6 cut(s) 5, 42, 126, 129, 174, 326
SspMI CTAG 5 cut(s) 269, 914, 1016, 1043, 1088
SstI GAGCTC 1 cut(s) 23
StyD4I CCNGG 1 cut(s) 621
StyI CCWWGG 6 cut(s) 157, 726, 913, 971, 1120, 1297
TaaI ACNGT 3 cut(s) 243, 964, 990
TaiI ACGT 1 cut(s) 521
TaqI TCGA 5 cut(s) 80, 153, 332, 597, 1274
TasI AATT 4 cut(s) 495, 634, 1224, 1373
TatI WGTACW 2 cut(s) 358, 1033
TauI GCSGC 2 cut(s) 131, 329
TfiI GAWTC 2 cut(s) 82, 438
Tru1I TTAA 4 cut(s) 284, 371, 584, 762
Tru9I TTAA 4 cut(s) 284, 371, 584, 762
TscAI CASTG 1 cut(s) 1182
TseFI GTSAC 1 cut(s) 1090
TseI GCWGC 6 cut(s) 108, 137, 686, 1220, 1292, 1313
Tsp45I GTSAC 1 cut(s) 1090
TspDTI ATGAA 4 cut(s) 46, 552, 1018, 1100
TspGWI ACGGA 4 cut(s) 13, 132, 294, 918
TspRI CASTG 1 cut(s) 1182
Van91I CCANNNNNTGG 1 cut(s) 732
Vha464I CTTAAG 1 cut(s) 761
VneI GTGCAC 1 cut(s) 908
VpaK11BI GGWCC 1 cut(s) 377
XceI RCATGY 3 cut(s) 609, 1327, 1331
XcmI CCANNNNNNNNNTGG 2 cut(s) 1262, 1362
XmaJI CCTAGG 1 cut(s) 913
XspI CTAG 5 cut(s) 269, 914, 1016, 1043, 1088
ZrmI AGTACT 1 cut(s) 360
Zsp2I ATGCAT 2 cut(s) 1027, 1329
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.