MD02G1196300.v1.1

SNARE-complex protein Syntaxin-18 N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0006035
Physical Location & Seq
Reverse (-)
18849437 .. 18852506
3070 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1196300.v1.1.491

Sequence Viewer

Length: 930 bp
ATGTCCAAAGTCAGGGATAGGATGGAAGATTTCAAAGATGCGGTGCGCCACTCTGCTATATCAATGGGTTACAACGAGTCCAAATTGGCAGCGATTATGGCAAAATTCATCATCCATGCACCCCGGCAGCGGTCGCCTTTCACCAGAGCTGCCCTCAAGACGCTTGAAAGCATAGGAGCTCTGGAACAGTTTATGTTGAAACATCGAAAGGATTATGTGGATCTGCATCGCACCACTGAACAGGAGAGGGATAGCATTGAGCATGAAGTTGGTGCTTTTATTAAAGCATGCCAAGAACAAATCGATGTTCTAAAAAATAGCATTAATGATGACGAGGCAAACTCAAAGGGCTGGCTTGGAATTAGGACTGACTACTCCAACGCTGATACCATAGCACACAAACATGGTGTGGTTTTGATTTTAAGTGAGAGACTCCATTCAGTCACGTCACGGTTTGATCAGCTAAGGGCCATACGCTTCCAAGATTCCATTAACAAAGCAACACCAAGAAGAAAACCTAAGCGGATTAAGACTTCAGCATCCACAGACACCTCTAACTCTAACAATTTGGAGCTCAGGGAACCTAATGAATTTCAACCCGAACCTCTAACAGTGCAACAACAACTCTTGGATGATGAAACACGTGCCCTTCAGGTAGAGCTGACTAGCCTTCTAGATGCGGTTCAGGAAACTGAAACTAAGATGGTGGAAATGTCTGCGCTGAATCACCTTATGTCCACACATGTTTTGCAACAAGCCCACCAAATAGAGCAACTGTACGATCAGGCAGTTGAAGCAACAAAGAACGTAGAGCTCGGCAACAAAGAGATCTCCAAAGCAATCCAGCGGAATAGCAGCAGCAGGACCTTTCTTTTTCTTTTCCTATTTGTACTTACGTTTTCGATTCTCTTTCTTGATTGGTATAGTTAA

Protein Analysis

310

Amino Acids

35.48

Weight (kDa)

6.43

Isoelectric Point (pI)

46.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Syntaxin-18_N PF10496 6 - 86 2.3e-15 SNARE-complex protein Syntaxin-18 N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 41, 130, 523, 680, 847
AclWI GGATC 1 cut(s) 228
AcsI RAATTY 2 cut(s) 104, 590
AcuI CTGAAG 2 cut(s) 519, 635
AcvI CACGTG 1 cut(s) 644
AfaI GTAC 2 cut(s) 779, 891
AfiI CCNNNNNNNGG 2 cut(s) 12, 129
AflIII ACRYGT 2 cut(s) 641, 742
AgsI TTSAA 5 cut(s) 34, 167, 199, 596, 794
AjiI CACGTC 1 cut(s) 447
AluBI AGCT 6 cut(s) 149, 179, 463, 574, 661, 814
AluI AGCT 6 cut(s) 149, 179, 463, 574, 661, 814
Alw21I GWGCWC 3 cut(s) 181, 576, 816
Alw26I GTCTC 1 cut(s) 424
AlwI GGATC 1 cut(s) 228
AoxI GGCC 1 cut(s) 468
ApeKI GCWGC 5 cut(s) 89, 127, 149, 855, 858
ApoI RAATTY 2 cut(s) 104, 590
AseI ATTAAT 1 cut(s) 324
AspLEI GCGC 2 cut(s) 48, 721
AspS9I GGNCC 2 cut(s) 468, 864
AsuC2I CCSGG 1 cut(s) 124
AsuHPI GGTGA 2 cut(s) 133, 719
AvaII GGWCC 1 cut(s) 864
BaeGI GKGCMC 1 cut(s) 649
BanII GRGCYC 3 cut(s) 181, 576, 816
BbrPI CACGTG 1 cut(s) 644
Bbv12I GWGCWC 3 cut(s) 181, 576, 816
BbvI GCAGC 5 cut(s) 101, 136, 139, 867, 870
BccI CCATC 2 cut(s) 16, 697
BclI TGATCA 1 cut(s) 457
BcnI CCSGG 1 cut(s) 124
BcoDI GTCTC 1 cut(s) 424
BfaI CTAG 2 cut(s) 666, 674
BglII AGATCT 1 cut(s) 828
BisI GCNGC 5 cut(s) 90, 128, 150, 856, 859
BlsI GCNGC 5 cut(s) 91, 129, 151, 857, 860
Bme1390I CCNGG 1 cut(s) 124
Bme18I GGWCC 1 cut(s) 864
BmgBI CACGTC 1 cut(s) 447
BmgT120I GGNCC 2 cut(s) 468, 864
BmiI GGNNCC 1 cut(s) 582
BmrFI CCNGG 1 cut(s) 124
BmsI GCATC 4 cut(s) 28, 235, 548, 667
BplI GAGNNNNNCTC 4 cut(s) 138, 170, 326, 358
Bpu10I CCTNAGC 3 cut(s) 464, 519, 575
BpuEI CTTGAG 1 cut(s) 140
BpuMI CCSGG 1 cut(s) 124
Bsa29I ATCGAT 1 cut(s) 303
BsaAI YACGTR 1 cut(s) 644
BsaBI GATNNNNATC 1 cut(s) 225
BsaJI CCNNGG 1 cut(s) 122
Bsc4I CCNNNNNNNGG 2 cut(s) 12, 129
Bse8I GATNNNNATC 1 cut(s) 225
BseCI ATCGAT 1 cut(s) 303
BseDI CCNNGG 1 cut(s) 122
BseGI GGATG 4 cut(s) 27, 111, 539, 637
BseJI GATNNNNATC 1 cut(s) 225
BseLI CCNNNNNNNGG 2 cut(s) 12, 129
BseMII CTCAG 1 cut(s) 589
BseSI GKGCMC 1 cut(s) 649
BseXI GCAGC 5 cut(s) 101, 136, 139, 867, 870
Bsh1285I CGRYCG 1 cut(s) 134
BshFI GGCC 1 cut(s) 470
BshVI ATCGAT 1 cut(s) 303
BsiEI CGRYCG 1 cut(s) 134
BsiHKAI GWGCWC 3 cut(s) 181, 576, 816
BsiSI CCGG 1 cut(s) 124
BslI CCNNNNNNNGG 2 cut(s) 12, 129
BsmAI GTCTC 1 cut(s) 424
BsnI GGCC 1 cut(s) 470
Bsp1286I GDGCHC 4 cut(s) 181, 576, 649, 816
Bsp143I GATC 4 cut(s) 220, 457, 781, 828
BspACI CCGC 5 cut(s) 41, 130, 523, 680, 847
BspANI GGCC 1 cut(s) 470
BspCNI CTCAG 1 cut(s) 588
BspDI ATCGAT 1 cut(s) 303
BspLI GGNNCC 1 cut(s) 582
BspPI GGATC 1 cut(s) 228
BssECI CCNNGG 1 cut(s) 122
BssMI GATC 4 cut(s) 220, 457, 781, 828
Bst4CI ACNGT 4 cut(s) 189, 453, 613, 777
BstBAI YACGTR 1 cut(s) 644
BstC8I GCNNGC 2 cut(s) 289, 353
BstDEI CTNAG 4 cut(s) 464, 519, 575, 699
BstF5I GGATG 4 cut(s) 27, 111, 539, 637
BstHHI GCGC 2 cut(s) 48, 721
BstKTI GATC 4 cut(s) 223, 460, 784, 831
BstMAI GTCTC 1 cut(s) 424
BstMBI GATC 4 cut(s) 220, 457, 781, 828
BstMCI CGRYCG 1 cut(s) 134
BstMWI GCNNNNNNNGC 3 cut(s) 98, 133, 794
BstNSI RCATGY 2 cut(s) 291, 746
BstSCI CCNGG 1 cut(s) 122
BstSLI GKGCMC 1 cut(s) 649
BstV1I GCAGC 5 cut(s) 101, 136, 139, 867, 870
BstX2I RGATCY 2 cut(s) 220, 828
BstYI RGATCY 2 cut(s) 220, 828
Bsu15I ATCGAT 1 cut(s) 303
BsuRI GGCC 1 cut(s) 470
BsuTUI ATCGAT 1 cut(s) 303
BtgZI GCGATG 1 cut(s) 212
BtrI CACGTC 1 cut(s) 447
BtsCI GGATG 4 cut(s) 27, 111, 539, 637
BtsIMutI CAGTG 2 cut(s) 234, 618
Cac8I GCNNGC 2 cut(s) 289, 353
CfoI GCGC 2 cut(s) 48, 721
Cfr13I GGNCC 2 cut(s) 468, 864
ClaI ATCGAT 1 cut(s) 303
CseI GACGC 1 cut(s) 169
Csp6I GTAC 2 cut(s) 778, 890
CviAII CATG 5 cut(s) 116, 263, 288, 404, 743
CviQI GTAC 2 cut(s) 778, 890
DdeI CTNAG 4 cut(s) 464, 519, 575, 699
DpnI GATC 4 cut(s) 222, 459, 783, 830
DpnII GATC 4 cut(s) 220, 457, 781, 828
Ecl136II GAGCTC 3 cut(s) 179, 574, 814
Eco24I GRGCYC 3 cut(s) 181, 576, 816
Eco47I GGWCC 1 cut(s) 864
Eco53kI GAGCTC 3 cut(s) 179, 574, 814
Eco57I CTGAAG 2 cut(s) 519, 635
Eco72I CACGTG 1 cut(s) 644
EcoICRI GAGCTC 3 cut(s) 179, 574, 814
EcoO109I RGGNCCY 1 cut(s) 864
EcoT38I GRGCYC 3 cut(s) 181, 576, 816
FaeI CATG 5 cut(s) 119, 266, 291, 407, 746
FatI CATG 5 cut(s) 115, 262, 287, 403, 742
FbaI TGATCA 1 cut(s) 457
Fnu4HI GCNGC 5 cut(s) 90, 128, 150, 856, 859
FokI GGATG 4 cut(s) 34, 98, 526, 644
FriOI GRGCYC 3 cut(s) 181, 576, 816
Fsp4HI GCNGC 5 cut(s) 90, 128, 150, 856, 859
FspBI CTAG 2 cut(s) 666, 674
GlaI GCGC 2 cut(s) 47, 720
GluI GCNGC 5 cut(s) 90, 128, 150, 856, 859
HaeIII GGCC 1 cut(s) 470
HapII CCGG 1 cut(s) 124
HgaI GACGC 1 cut(s) 169
HhaI GCGC 2 cut(s) 48, 721
Hin1II CATG 5 cut(s) 119, 266, 291, 407, 746
Hin6I GCGC 2 cut(s) 46, 719
HinP1I GCGC 2 cut(s) 46, 719
HinfI GANTC 5 cut(s) 77, 432, 485, 724, 904
HpaII CCGG 1 cut(s) 124
HphI GGTGA 2 cut(s) 133, 719
Hpy166II GTNNAC 1 cut(s) 738
Hpy188III TCNNGA 5 cut(s) 157, 182, 674, 686, 914
Hpy8I GTNNAC 1 cut(s) 738
HpyAV CCTTC 2 cut(s) 659, 680
HpyCH4III ACNGT 4 cut(s) 189, 453, 613, 777
HpyCH4IV ACGT 4 cut(s) 446, 643, 807, 896
HpyCH4V TGCA 4 cut(s) 119, 226, 616, 751
HpyF10VI GCNNNNNNNGC 3 cut(s) 98, 133, 794
HpyF3I CTNAG 4 cut(s) 464, 519, 575, 699
HpySE526I ACGT 4 cut(s) 446, 643, 807, 896
Hsp92II CATG 5 cut(s) 119, 266, 291, 407, 746
HspAI GCGC 2 cut(s) 46, 719
Ksp22I TGATCA 1 cut(s) 457
Kzo9I GATC 4 cut(s) 220, 457, 781, 828
LmnI GCTCC 2 cut(s) 176, 571
Lsp1109I GCAGC 5 cut(s) 101, 136, 139, 867, 870
LweI GCATC 4 cut(s) 28, 235, 548, 667
MaeI CTAG 2 cut(s) 666, 674
MaeII ACGT 4 cut(s) 446, 643, 807, 896
MaeIII GTNAC 3 cut(s) 68, 442, 447
MalI GATC 4 cut(s) 222, 459, 783, 830
MboI GATC 4 cut(s) 220, 457, 781, 828
MboII GAAGA 2 cut(s) 38, 522
MflI RGATCY 2 cut(s) 220, 828
MhlI GDGCHC 4 cut(s) 181, 576, 649, 816
MluCI AATT 5 cut(s) 83, 104, 360, 565, 590
MlyI GAGTC 2 cut(s) 86, 426
MmeI TCCRAC 1 cut(s) 402
MnlI CCTC 5 cut(s) 164, 240, 328, 562, 615
MseI TTAA 6 cut(s) 282, 324, 422, 492, 528, 928
MslI CAYNNNNRTG 1 cut(s) 402
MspA1I CMGCKG 2 cut(s) 130, 847
MspI CCGG 1 cut(s) 124
MspR9I CCNGG 1 cut(s) 124
MwoI GCNNNNNNNGC 3 cut(s) 98, 133, 794
NciI CCSGG 1 cut(s) 124
NdeII GATC 4 cut(s) 220, 457, 781, 828
NlaIII CATG 5 cut(s) 119, 266, 291, 407, 746
NlaIV GGNNCC 1 cut(s) 582
NmeAIII GCCGAG 1 cut(s) 795
NmuCI GTSAC 2 cut(s) 442, 447
NspI RCATGY 2 cut(s) 291, 746
PaeI GCATGC 1 cut(s) 291
PciI ACATGT 1 cut(s) 742
PfeI GAWTC 3 cut(s) 485, 724, 904
PkrI GCNGC 5 cut(s) 91, 129, 151, 857, 860
PleI GAGTC 2 cut(s) 85, 426
PmaCI CACGTG 1 cut(s) 644
PmlI CACGTG 1 cut(s) 644
PpsI GAGTC 2 cut(s) 85, 426
Ppu21I YACGTR 1 cut(s) 644
PpuMI RGGWCCY 1 cut(s) 864
PscI ACATGT 1 cut(s) 742
PshBI ATTAAT 1 cut(s) 324
Psp124BI GAGCTC 3 cut(s) 181, 576, 816
Psp5II RGGWCCY 1 cut(s) 864
PspCI CACGTG 1 cut(s) 644
PspN4I GGNNCC 1 cut(s) 582
PspPI GGNCC 2 cut(s) 468, 864
PspPPI RGGWCCY 1 cut(s) 864
PsuI RGATCY 2 cut(s) 220, 828
RsaI GTAC 2 cut(s) 779, 891
RsaNI GTAC 2 cut(s) 778, 890
RseI CAYNNNNRTG 1 cut(s) 402
SacI GAGCTC 3 cut(s) 181, 576, 816
SaqAI TTAA 6 cut(s) 282, 324, 422, 492, 528, 928
SatI GCNGC 5 cut(s) 90, 128, 150, 856, 859
Sau3AI GATC 4 cut(s) 220, 457, 781, 828
Sau96I GGNCC 2 cut(s) 468, 864
SchI GAGTC 2 cut(s) 86, 426
ScrFI CCNGG 1 cut(s) 124
SduI GDGCHC 4 cut(s) 181, 576, 649, 816
SfaNI GCATC 4 cut(s) 28, 235, 548, 667
SinI GGWCC 1 cut(s) 864
SmiMI CAYNNNNRTG 1 cut(s) 402
SmlI CTYRAG 1 cut(s) 155
SmoI CTYRAG 1 cut(s) 155
SphI GCATGC 1 cut(s) 291
Sse9I AATT 5 cut(s) 83, 104, 360, 565, 590
SsiI CCGC 5 cut(s) 41, 130, 523, 680, 847
SspMI CTAG 2 cut(s) 666, 674
SstI GAGCTC 3 cut(s) 181, 576, 816
StyD4I CCNGG 1 cut(s) 122
TaaI ACNGT 4 cut(s) 189, 453, 613, 777
TaiI ACGT 4 cut(s) 449, 646, 810, 899
TaqI TCGA 3 cut(s) 205, 303, 902
TasI AATT 5 cut(s) 83, 104, 360, 565, 590
TatI WGTACW 1 cut(s) 889
TfiI GAWTC 3 cut(s) 485, 724, 904
Tru1I TTAA 6 cut(s) 282, 324, 422, 492, 528, 928
Tru9I TTAA 6 cut(s) 282, 324, 422, 492, 528, 928
TscAI CASTG 2 cut(s) 241, 618
TseFI GTSAC 2 cut(s) 442, 447
TseI GCWGC 5 cut(s) 89, 127, 149, 855, 858
Tsp45I GTSAC 2 cut(s) 442, 447
TspDTI ATGAA 4 cut(s) 97, 279, 603, 651
TspRI CASTG 2 cut(s) 241, 618
VpaK11BI GGWCC 1 cut(s) 864
VspI ATTAAT 1 cut(s) 324
XapI RAATTY 2 cut(s) 104, 590
XbaI TCTAGA 1 cut(s) 673
XceI RCATGY 2 cut(s) 291, 746
XspI CTAG 2 cut(s) 666, 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.