Rorug01G0222000

SNARE-complex protein Syntaxin-18 N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0006035
Physical Location & Seq
Forward (+)
31851012 .. 31888332
37321 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0222000.1

Sequence Viewer

Length: 3927 bp
ATGGCCGATGTTAGAGCAGCTCACCACAAAACTTCATCTTCCAATACTGCATCTCCTTTTCCCAGTAGGTATCATGTGTTCTTGAGTTTCAGGGGCCAAGACACTCGCAAAACTTTTACCGATCACCTCTATACAGCCTTTAAGGACCAAGGCTTTCGCACCTTCCTAGATGACGATGAACTCGAGAGAGGAGAAGATATTAACCCTGGACTTGAGAAAGCGATTCAACAGTCACGAAGTTCTGTCATTGTGTTCTCAAAAGATTACGCGTCTTCCAGGTGGTGCCTTAACGAGCTGTTGATGATCCTTGAACGCAAGAGGACCTCAGATCATGTAGTTTTACCAGTCTTCTACAACGTGGAACCATCCCAAGTGAGGAAGCAGACCGGAAGTTTTGGGGAGGCATTTGCCAGACACCAAAAGACTCAACCGTCGGACAAGGTGAAAGAATGGAAGGACGCACTTACGAAAGTTGCAGATCTTGGAGGGATGGTCTTACGGAACCAAGATGACGGGTATGAGTCAAAGTTCATCAAAAAAATTGTTAGAGTGATTGCAGACAAGCTAAGTCGCCCACTCTTAAGTGTTCCTCAAAACCTGATTGGAATCCATTCTCAAGTCCAAAGCATTAACTCATGGGTGGAAGATGGATCAACTGATGTTGGTATACTTGCAATTTATGGTATGTCTGGCATAGGGAAGACGACCATTGCAAAATCTGTTTATAATTCAAACTTTGGAAGATTTGAAGGAAGCAGCTTCCTTGAAAATATTAGAGAAACTTCAGAACAACCAAATGGTTTAGTTCAAATACAAATACAACTTCTTTCTAATATTTTGAATGGAAGAAAAGTGAAGATTCACAGTGTCAGTGAGGGAATAATTAAGATCCAAGATGCCATAAGCTCTAAGAGAATTCTTCTTGTCCTTGATGATGTTGATGATTTGGACGAATTAAAGGCCGTACTTAGCATGCAAGATCAGTTTTATCCCGGGAGTAAAATCATCATGACAACTAGGCGCAGAAGATTGTTATCGGCTCATCAAGTCACTAAAGTTCATATTGTTGAAACTCTGGGATACAATGAATCATTGGAGCTCTTCAGTTGGCATGCATTTGGACAAGACCATCCTATTGAAGGTTACATGAAACATTCAGAAAAGGTAGTAGATCACAGCGGAGGACTTCCTTTAGCTCTTCAAGTTTTGGGTTCTTCTCTATCCGGGGAAAGTATAGCTGTATGGGAAAGTGCATTGGAGAAATTAGAAGCTATCCCAAACAGTGAAATTATGAAAAAGCTCAGAATAAGCTACGATGGTTTACAAGATGACCATGACAAACAATTATTCCTACATCTTGTCTGCTTCTTTATTGGAAAAGACAAAGACTTCATAGTTACATTACTCGACGGATGTGATTTCTACACGGTTGTTGGCATTCAAAATCTCATTGACAGATGTTTAGTGACAATTCATAGATATAATAACAAGCTGCAGATGCATGACATGATTCGTGACATGGGAAGAGGAATTGTTCGGCTAGAATCAGAGGAGCCAGAGAGACGCAGCAGACTGTGGCATCATAAGGATTCACTCAGAGTATTGAGAGAGAAAAATGGAACACATACAATTCAGGGTCTTGTCCTCAACATGCATATGCAACAAGGAGACGGCCCTTCAAGGATAGTCTTGGAAACCAATGCATTTGCAAGAATGCATAAACTGAGATTCCTCCATCTTAGCCATGTACAACTCAAACACTGTTATAAAGAATTCCCAACAGCACTAAAATGGTTATGTTGGCATGAATTGTCTCTGGATTCTATACCGGATGATTTTTCTTTAGAAGGCCTGGTTATTCTTGAAATGCAGCATAGCAGCTTGAGACAAGTCTGGAACGGAACAAAATGTCTTCCATTATTGAAGATTATTGATCTCAGCCATTCTCATAGCCTTATAGAAACCACTGACCTCTCATTTTTCCCCAATCTAGAGAAACTGATTCTTGTAGATTGTGTAAGCCTGATTGATGTCCATATATCCATTGGAAACCTGGAAAGACTTGTTTACTTAAATCTGAAAGATTGCAAAAGTATTAGGATGCTCCCGAACAACATATGTAGGCTAAAATTACTTGAAGCACTTATTATATCTGGCTGCTCAAATCTGAAGGAGCTACCAATTGAGATGATGAGGTACATGACATATCTGAAAGTGTTTGAAGCAGACGGAATTCCTATAAATGAGTTAGTCACTACTAGCGAGGAGGTCAAATCATGGCCAGGAAGAAGTTCATCTGTTTTCTGGACTTGTTTACCATGCTCTTTAGTGGAATTAAGTCTTGCAGGATGCAATCTTTCCAATGAATATTTTCCTTGGGAATTTCATAACCAACACTCATTGAAAATATTAAATTTAGCAAATAATCCAATTATTAGCCTCCCGAATTGCATCAAAGTTCTAAAGAGGCTTTATAAGCTCGGTTTTTATGGCTGCAATAAGCTCAAATCGCTTGTAGGGCTACCAAAGGTAGATTTCATATTGGAGGTGACAAATTGCTCATCACTGGAAAGAATAACATATCAATCGTTTTCATGCAAAGAAAACAAGTTGTGGCATACTTCGAGCTTACCCGGTCTAGTTGAGGTTGAGTACCTATTCAAGTTAGAACCCATTGAAAAAGTTGATGTAGAAATGCTTAACCTTTTGGGCTTGTGCAACGTGGAATCCATGCCAACCATTCAGATCCACCCAGCTTTTCATTATATTCCTCAAGAAAGACCCGTTCAGGGACTGTCTGAATTTGGTATATTCAGCACATTTCTTCCTGGGAATCAGGTTCCAGGCCAGTTCAGCTATAGAAATAAAGAGTCATCGTCAAAATCTTTTACTGTGCCTTCACTTCCCAATCTCAGGAGCCGAGGCTGGAACATCTTCGTTGTCTATGCCTTCTGTTTTCACGTAGACTATAGTGGACCTGATGATGATGTACCCGACGACTCTCCCATGATTATTAAAGTCACACATAAGAGCAAGAGTCTAAAGTGGATCTATGGCCCATCATGCCATGGTGTTCTGCCTGTGGGAGAAGACATAATATGGTTAAGCAATTGGAATATGGAAAATGAATTACAAGGTGGCGATGAAGTTATTCTTTCGGTATTTGTACATCATATGCTTGTGTTAAAGGAGTGGGGCATCCAAATTCTGCCAGAGCAAGAAGAGAATATGAGTACGCAGCGCAACAGGCCAGGTTCATTTGGTTATCCAATAGTCATTGGTGGAGATTTGTCACTGTATGAGCTGATACCAAGAATATACTTGCTCCTCGGTAAACCTAACTCCTCAAGAGAAGATATTGCTTACTGTTGGTATAGTCACATTGTTGACGACTCTGATGAAGATACAGACCGAGAAGAAGAGGAAGAGGAAGAAGCAATGAGCGCTTTCACTGATAGCTTTGGTCACAGAAGCTGGGAGATATCAAAAAAATCATCAAAAAAGGATTGTTGTGATGACGATGATGTTGATTTTGAAATCAACCTAGAACGTGATGTTGACATTGTTGAACTAGATGGAAATCCCAGTACCACTACCCAGAAAAGTAGCGGAAAGAGAAAACGCTCATCTTTTACTAACGTGTGCGCCGAGGGTTCAGGGAACAGAAATGAATTGCTTGAGAAAGAAGTTGCTGATTCCGCGCCATTACGTGGTAATGATGAAGTAGCTCACCCAGTTGCAAAGGATGTTGTTGCAGATGAAGAATTGATGCAATGCTTTGACATTCTGAACACCATGGATCCGGAAATTGATGATGTTAGTTACTCCAAAGCTCTGATGATTTTCCACCAGGATGCTACTTTGAGAAAGATGTTTGTGCGAATGCCTGGTAAGAGGCGAACTAGTTTCATTCTTCACCTTATATAG

Protein Analysis

1308

Amino Acids

148.92

Weight (kDa)

5.8

Isoelectric Point (pI)

45.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 24 - 190 6.3e-54 TIR domain
TIR_2 PF13676 26 - 122 1.3e-16 TIR domain
NB-ARC PF00931 208 - 374 4e-21 NB-ARC domain
WHD_ROQ1 PF23282 444 - 515 2.1e-16 Disease resistance protein Roq1-like, winged-helix domain
LRR_14 PF23598 655 - 739 1.4e-06 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 726, 1767, 2475
AasI GACNNNNNNGTC 1 cut(s) 430
AccB1I GGYRCC 1 cut(s) 282
AccB7I CCANNNNNTGG 1 cut(s) 3710
AccI GTMKAC 2 cut(s) 667, 2964
AccII CGCG 2 cut(s) 269, 3701
AccIII TCCGGA 1 cut(s) 3802
AciI CCGC 3 cut(s) 1179, 3609, 3699
AclWI GGATC 7 cut(s) 298, 658, 883, 2740, 3056, 3794, 3807
AcoI YGGCCR 2 cut(s) 3, 2279
AcsI RAATTY 7 cut(s) 915, 1772, 2232, 2381, 2413, 2801, 3204
AcuI CTGAAG 3 cut(s) 768, 1087, 2189
AfaI GTAC 8 cut(s) 966, 1749, 2198, 2654, 2991, 3168, 3235, 3589
AfeI AGCGCT 1 cut(s) 3445
AfiI CCNNNNNNNGG 5 cut(s) 375, 1139, 2789, 2913, 3710
AflII CTTAAG 1 cut(s) 580
AflIII ACRYGT 2 cut(s) 267, 3639
AhlI ACTAGT 1 cut(s) 3902
Alw21I GWGCWC 1 cut(s) 1101
Alw26I GTCTC 4 cut(s) 1555, 1662, 1818, 1879
AlwI GGATC 7 cut(s) 298, 658, 883, 2740, 3056, 3794, 3807
AlwNI CAGNNNCTG 2 cut(s) 2794, 3474
Ama87I CYCGRG 2 cut(s) 182, 992
Aor13HI TCCGGA 1 cut(s) 3802
Aor51HI AGCGCT 1 cut(s) 3445
AoxI GGCC 9 cut(s) 3, 94, 960, 1672, 1849, 2279, 2845, 3055, 3248
ApeKI GCWGC 9 cut(s) 17, 756, 1492, 1566, 1870, 1878, 2157, 2493, 3238
ApoI RAATTY 7 cut(s) 915, 1772, 2232, 2381, 2413, 2801, 3204
Asp700I GAANNNNTTC 5 cut(s) 610, 2290, 2370, 2933, 3150
AspLEI GCGC 5 cut(s) 1023, 3243, 3446, 3647, 3703
AspS9I GGNCC 6 cut(s) 94, 145, 321, 1673, 2975, 3056
AsuC2I CCSGG 4 cut(s) 993, 994, 1225, 2634
AsuHPI GGTGA 6 cut(s) 14, 116, 454, 2560, 3722, 3908
AvaI CYCGRG 2 cut(s) 182, 992
AvaII GGWCC 3 cut(s) 145, 321, 2975
BalI TGGCCA 1 cut(s) 2281
BamHI GGATCC 1 cut(s) 3799
BanI GGYRCC 1 cut(s) 282
BanII GRGCYC 1 cut(s) 1101
BbsI GAAGAC 5 cut(s) 264, 340, 707, 1904, 3096
Bbv12I GWGCWC 1 cut(s) 1101
BbvI GCAGC 9 cut(s) 29, 768, 1479, 1578, 1882, 1890, 2144, 2480, 3250
BccI CCATC 8 cut(s) 373, 484, 641, 1137, 1310, 1743, 3067, 3569
BceAI ACGGC 2 cut(s) 947, 1687
BciVI GTATCC 1 cut(s) 1073
BcnI CCSGG 4 cut(s) 993, 994, 1225, 2634
BcoDI GTCTC 4 cut(s) 1555, 1662, 1818, 1879
BcuI ACTAGT 1 cut(s) 3902
BfaI CTAG 9 cut(s) 167, 1017, 1541, 1991, 2259, 2639, 3545, 3572, 3903
BfmI CTRYAG 3 cut(s) 1493, 2857, 2968
BfoI RGCGCY 1 cut(s) 3447
BfrI CTTAAG 1 cut(s) 580
BfuI GTATCC 1 cut(s) 1073
BglII AGATCT 1 cut(s) 478
BisI GCNGC 9 cut(s) 18, 757, 1493, 1567, 1871, 1879, 2158, 2494, 3239
BlsI GCNGC 9 cut(s) 19, 758, 1494, 1568, 1872, 1880, 2159, 2495, 3240
Bme18I GGWCC 3 cut(s) 145, 321, 2975
BmeT110I CYCGRG 2 cut(s) 182, 992
BmgT120I GGNCC 6 cut(s) 94, 145, 321, 1673, 2975, 3056
BmiI GGNNCC 8 cut(s) 95, 284, 363, 503, 1554, 2841, 2918, 3801
BmrI ACTGGG 3 cut(s) 57, 3579, 3728
BmuI ACTGGG 3 cut(s) 57, 3579, 3728
BpiI GAAGAC 5 cut(s) 264, 340, 707, 1904, 3096
BpuEI CTTGAG 7 cut(s) 103, 233, 600, 1903, 2757, 3331, 3698
BpuMI CCSGG 4 cut(s) 993, 994, 1225, 2634
BsaAI YACGTR 2 cut(s) 2962, 3710
BsaBI GATNNNNATC 3 cut(s) 605, 1033, 3579
BsaWI WCCGGW 3 cut(s) 386, 1828, 3802
BsaXI ACNNNNNCTCC 4 cut(s) 37, 67, 477, 507
Bsc4I CCNNNNNNNGG 5 cut(s) 375, 1139, 2789, 2913, 3710
Bse1I ACTGG 6 cut(s) 63, 344, 2571, 2848, 3585, 3734
Bse3DI GCAATG 3 cut(s) 708, 3444, 3779
Bse8I GATNNNNATC 3 cut(s) 605, 1033, 3579
BseAI TCCGGA 1 cut(s) 3802
BseJI GATNNNNATC 3 cut(s) 605, 1033, 3579
BseLI CCNNNNNNNGG 5 cut(s) 375, 1139, 2789, 2913, 3710
BseMI GCAATG 3 cut(s) 708, 3444, 3779
BseMII CTCAG 6 cut(s) 339, 1315, 1609, 1715, 1951, 2926
BseNI ACTGG 6 cut(s) 63, 344, 2571, 2848, 3585, 3734
BseRI GAGGAG 5 cut(s) 204, 1565, 2279, 3317, 3334
BseXI GCAGC 9 cut(s) 29, 768, 1479, 1578, 1882, 1890, 2144, 2480, 3250
BseYI CCCAGC 2 cut(s) 2752, 3474
Bsh1236I CGCG 2 cut(s) 269, 3701
BshFI GGCC 9 cut(s) 5, 96, 962, 1674, 1851, 2281, 2847, 3057, 3250
BshNI GGYRCC 1 cut(s) 282
BsiHKAI GWGCWC 1 cut(s) 1101
BsiHKCI CYCGRG 2 cut(s) 182, 992
BsiSI CCGG 6 cut(s) 387, 993, 1224, 1829, 2634, 3803
BslFI GGGAC 1 cut(s) 2805
BslI CCNNNNNNNGG 5 cut(s) 375, 1139, 2789, 2913, 3710
BsmAI GTCTC 4 cut(s) 1555, 1662, 1818, 1879
BsmBI CGTCTC 2 cut(s) 1555, 1662
BsmFI GGGAC 1 cut(s) 2805
BsmI GAATGC 3 cut(s) 1437, 1719, 3888
BsnI GGCC 9 cut(s) 5, 96, 962, 1674, 1851, 2281, 2847, 3057, 3250
BsoBI CYCGRG 2 cut(s) 182, 992
Bsp1286I GDGCHC 1 cut(s) 1101
Bsp13I TCCGGA 1 cut(s) 3802
Bsp1407I TGTACA 2 cut(s) 1747, 3166
Bsp19I CCATGG 2 cut(s) 3067, 3795
BspACI CCGC 3 cut(s) 1179, 3609, 3699
BspANI GGCC 9 cut(s) 5, 96, 962, 1674, 1851, 2281, 2847, 3057, 3250
BspCNI CTCAG 6 cut(s) 338, 1314, 1608, 1716, 1950, 2925
BspEI TCCGGA 1 cut(s) 3802
BspFNI CGCG 2 cut(s) 269, 3701
BspHI TCATGA 1 cut(s) 1008
BspLI GGNNCC 8 cut(s) 95, 284, 363, 503, 1554, 2841, 2918, 3801
BspMAI CTGCAG 1 cut(s) 1497
BspPI GGATC 7 cut(s) 298, 658, 883, 2740, 3056, 3794, 3807
BspQI GCTCTTC 2 cut(s) 1106, 1203
BspT107I GGYRCC 1 cut(s) 282
BspTI CTTAAG 1 cut(s) 580
BsrDI GCAATG 3 cut(s) 708, 3444, 3779
BsrGI TGTACA 2 cut(s) 1747, 3166
BsrI ACTGG 6 cut(s) 63, 344, 2571, 2848, 3585, 3734
BssNAI GTATAC 1 cut(s) 668
BssT1I CCWWGG 4 cut(s) 148, 2375, 3067, 3795
Bst1107I GTATAC 1 cut(s) 668
Bst6I CTCTTC 6 cut(s) 1106, 1203, 1519, 3216, 3414, 3420
BstAFI CTTAAG 1 cut(s) 580
BstAUI TGTACA 2 cut(s) 1747, 3166
BstBAI YACGTR 2 cut(s) 2962, 3710
BstC8I GCNNGC 2 cut(s) 974, 1113
BstDSI CCRYGG 2 cut(s) 3067, 3795
BstENI CCTNNNNNAGG 1 cut(s) 1137
BstFNI CGCG 2 cut(s) 269, 3701
BstH2I RGCGCY 1 cut(s) 3447
BstHHI GCGC 5 cut(s) 1023, 3243, 3446, 3647, 3703
BstMAI GTCTC 4 cut(s) 1555, 1662, 1818, 1879
BstMWI GCNNNNNNNGC 9 cut(s) 1498, 2476, 2509, 2518, 2853, 3063, 3247, 3443, 3698
BstNSI RCATGY 3 cut(s) 976, 1115, 1654
BstSFI CTRYAG 3 cut(s) 1493, 2857, 2968
BstUI CGCG 2 cut(s) 269, 3701
BstV1I GCAGC 9 cut(s) 29, 768, 1479, 1578, 1882, 1890, 2144, 2480, 3250
BstV2I GAAGAC 5 cut(s) 264, 340, 707, 1904, 3096
BstX2I RGATCY 5 cut(s) 478, 888, 2745, 3048, 3799
BstYI RGATCY 5 cut(s) 478, 888, 2745, 3048, 3799
BstZ17I GTATAC 1 cut(s) 668
BsuI GTATCC 1 cut(s) 1073
BsuRI GGCC 9 cut(s) 5, 96, 962, 1674, 1851, 2281, 2847, 3057, 3250
BtgI CCRYGG 2 cut(s) 3067, 3795
BtgZI GCGATG 1 cut(s) 3156
BtsIMutI CAGTG 8 cut(s) 871, 877, 1288, 1759, 1965, 2564, 3293, 3450
Cac8I GCNNGC 2 cut(s) 974, 1113
CaiI CAGNNNCTG 2 cut(s) 2794, 3474
CciI TCATGA 1 cut(s) 1008
CfoI GCGC 5 cut(s) 1023, 3243, 3446, 3647, 3703
Cfr13I GGNCC 6 cut(s) 94, 145, 321, 1673, 2975, 3056
Cfr9I CCCGGG 1 cut(s) 992
CseI GACGC 3 cut(s) 258, 467, 1572
Csp6I GTAC 8 cut(s) 965, 1748, 2197, 2653, 2990, 3167, 3234, 3588
CviQI GTAC 8 cut(s) 965, 1748, 2197, 2653, 2990, 3167, 3234, 3588
DrdI GACNNNNNNGTC 1 cut(s) 430
DseDI GACNNNNNNGTC 1 cut(s) 430
EaeI YGGCCR 2 cut(s) 3, 2279
Eam1104I CTCTTC 6 cut(s) 1106, 1203, 1519, 3216, 3414, 3420
EarI CTCTTC 6 cut(s) 1106, 1203, 1519, 3216, 3414, 3420
Ecl136II GAGCTC 1 cut(s) 1099
Eco130I CCWWGG 4 cut(s) 148, 2375, 3067, 3795
Eco147I AGGCCT 1 cut(s) 1851
Eco24I GRGCYC 1 cut(s) 1101
Eco32I GATATC 1 cut(s) 3483
Eco47I GGWCC 3 cut(s) 145, 321, 2975
Eco47III AGCGCT 1 cut(s) 3445
Eco53kI GAGCTC 1 cut(s) 1099
Eco57I CTGAAG 3 cut(s) 768, 1087, 2189
Eco88I CYCGRG 2 cut(s) 182, 992
EcoICRI GAGCTC 1 cut(s) 1099
EcoNI CCTNNNNNAGG 1 cut(s) 1137
EcoO109I RGGNCCY 1 cut(s) 321
EcoRI GAATTC 3 cut(s) 915, 1772, 2232
EcoRV GATATC 1 cut(s) 3483
EcoT14I CCWWGG 4 cut(s) 148, 2375, 3067, 3795
EcoT22I ATGCAT 5 cut(s) 1117, 1503, 1656, 1705, 1719
EcoT38I GRGCYC 1 cut(s) 1101
ErhI CCWWGG 4 cut(s) 148, 2375, 3067, 3795
Esp3I CGTCTC 2 cut(s) 1555, 1662
FalI AAGNNNNNCTT 2 cut(s) 3138, 3170
FaqI GGGAC 1 cut(s) 2805
FauNDI CATATG 3 cut(s) 1656, 2117, 3174
FblI GTMKAC 2 cut(s) 667, 2964
Fnu4HI GCNGC 9 cut(s) 18, 757, 1493, 1567, 1871, 1879, 2158, 2494, 3239
FriOI GRGCYC 1 cut(s) 1101
Fsp4HI GCNGC 9 cut(s) 18, 757, 1493, 1567, 1871, 1879, 2158, 2494, 3239
FspBI CTAG 9 cut(s) 167, 1017, 1541, 1991, 2259, 2639, 3545, 3572, 3903
GlaI GCGC 5 cut(s) 1022, 3242, 3445, 3646, 3702
GluI GCNGC 9 cut(s) 18, 757, 1493, 1567, 1871, 1879, 2158, 2494, 3239
GsaI CCCAGC 2 cut(s) 2756, 3478
HaeII RGCGCY 1 cut(s) 3447
HaeIII GGCC 9 cut(s) 5, 96, 962, 1674, 1851, 2281, 2847, 3057, 3250
HapII CCGG 6 cut(s) 387, 993, 1224, 1829, 2634, 3803
HgaI GACGC 3 cut(s) 258, 467, 1572
HhaI GCGC 5 cut(s) 1023, 3243, 3446, 3647, 3703
Hin6I GCGC 5 cut(s) 1021, 3241, 3444, 3645, 3701
HinP1I GCGC 5 cut(s) 1021, 3241, 3444, 3645, 3701
HincII GTYRAC 2 cut(s) 3388, 3559
HindII GTYRAC 2 cut(s) 3388, 3559
HpaII CCGG 6 cut(s) 387, 993, 1224, 1829, 2634, 3803
HphI GGTGA 6 cut(s) 14, 116, 454, 2560, 3722, 3908
Hpy166II GTNNAC 9 cut(s) 668, 1322, 2068, 2315, 2965, 2975, 3335, 3388, 3559
Hpy8I GTNNAC 9 cut(s) 668, 1322, 2068, 2315, 2965, 2975, 3335, 3388, 3559
Hpy99I CGWCG 3 cut(s) 436, 1412, 2999
HpyAV CCTTC 9 cut(s) 172, 448, 743, 1133, 1686, 1841, 2164, 2907, 2959
HpyCH4IV ACGT 6 cut(s) 357, 2721, 2961, 3550, 3639, 3709
HpyF10VI GCNNNNNNNGC 9 cut(s) 1498, 2476, 2509, 2518, 2853, 3063, 3247, 3443, 3698
HpySE526I ACGT 6 cut(s) 357, 2721, 2961, 3550, 3639, 3709
HspAI GCGC 5 cut(s) 1021, 3241, 3444, 3645, 3701
Kpn2I TCCGGA 1 cut(s) 3802
LguI GCTCTTC 2 cut(s) 1106, 1203
LmnI GCTCC 6 cut(s) 1096, 1552, 2109, 2173, 2916, 3330
Lsp1109I GCAGC 9 cut(s) 29, 768, 1479, 1578, 1882, 1890, 2144, 2480, 3250
MaeI CTAG 9 cut(s) 167, 1017, 1541, 1991, 2259, 2639, 3545, 3572, 3903
MaeII ACGT 6 cut(s) 357, 2721, 2961, 3550, 3639, 3709
MfeI CAATTG 2 cut(s) 2181, 3109
MflI RGATCY 5 cut(s) 478, 888, 2745, 3048, 3799
MhlI GDGCHC 1 cut(s) 1101
MlsI TGGCCA 1 cut(s) 2281
MluI ACGCGT 1 cut(s) 267
MluNI TGGCCA 1 cut(s) 2281
MlyI GAGTC 6 cut(s) 418, 530, 2879, 2993, 3046, 3386
MmeI TCCRAC 1 cut(s) 414
Mox20I TGGCCA 1 cut(s) 2281
Mph1103I ATGCAT 5 cut(s) 1117, 1503, 1656, 1705, 1719
MroI TCCGGA 1 cut(s) 3802
MroXI GAANNNNTTC 5 cut(s) 610, 2290, 2370, 2933, 3150
MscI TGGCCA 1 cut(s) 2281
MslI CAYNNNNRTG 3 cut(s) 1655, 1789, 3852
Msp20I TGGCCA 1 cut(s) 2281
MspA1I CMGCKG 1 cut(s) 1179
MspCI CTTAAG 1 cut(s) 580
MspI CCGG 6 cut(s) 387, 993, 1224, 1829, 2634, 3803
MunI CAATTG 2 cut(s) 2181, 3109
Mva1269I GAATGC 3 cut(s) 1437, 1719, 3888
MvnI CGCG 2 cut(s) 269, 3701
MwoI GCNNNNNNNGC 9 cut(s) 1498, 2476, 2509, 2518, 2853, 3063, 3247, 3443, 3698
NciI CCSGG 4 cut(s) 993, 994, 1225, 2634
NcoI CCATGG 2 cut(s) 3067, 3795
NdeI CATATG 3 cut(s) 1656, 2117, 3174
NlaIV GGNNCC 8 cut(s) 95, 284, 363, 503, 1554, 2841, 2918, 3801
NmeAIII GCCGAG 2 cut(s) 2945, 3673
NsiI ATGCAT 5 cut(s) 1117, 1503, 1656, 1705, 1719
NspI RCATGY 3 cut(s) 976, 1115, 1654
PaeI GCATGC 2 cut(s) 976, 1115
PaeR7I CTCGAG 1 cut(s) 182
PagI TCATGA 1 cut(s) 1008
PceI AGGCCT 1 cut(s) 1851
PciSI GCTCTTC 2 cut(s) 1106, 1203
PcsI WCGNNNNNNNCGW 2 cut(s) 180, 3645
PctI GAATGC 3 cut(s) 1437, 1719, 3888
PdmI GAANNNNTTC 5 cut(s) 610, 2290, 2370, 2933, 3150
PflMI CCANNNNNTGG 1 cut(s) 3710
PkrI GCNGC 9 cut(s) 19, 758, 1494, 1568, 1872, 1880, 2159, 2495, 3240
PleI GAGTC 6 cut(s) 418, 529, 2878, 2993, 3045, 3386
PpsI GAGTC 6 cut(s) 418, 529, 2878, 2993, 3045, 3386
Ppu21I YACGTR 2 cut(s) 2962, 3710
PpuMI RGGWCCY 1 cut(s) 321
PsiI TTATAA 3 cut(s) 726, 1767, 2475
Psp124BI GAGCTC 1 cut(s) 1101
Psp5II RGGWCCY 1 cut(s) 321
PspFI CCCAGC 2 cut(s) 2752, 3474
PspN4I GGNNCC 8 cut(s) 95, 284, 363, 503, 1554, 2841, 2918, 3801
PspPI GGNCC 6 cut(s) 94, 145, 321, 1673, 2975, 3056
PspPPI RGGWCCY 1 cut(s) 321
PsrI GAACNNNNNNTAC 2 cut(s) 62, 94
PstI CTGCAG 1 cut(s) 1497
PstNI CAGNNNCTG 2 cut(s) 2794, 3474
PsuI RGATCY 5 cut(s) 478, 888, 2745, 3048, 3799
RsaI GTAC 8 cut(s) 966, 1749, 2198, 2654, 2991, 3168, 3235, 3589
RsaNI GTAC 8 cut(s) 965, 1748, 2197, 2653, 2990, 3167, 3234, 3588
RseI CAYNNNNRTG 3 cut(s) 1655, 1789, 3852
SacI GAGCTC 1 cut(s) 1101
SapI GCTCTTC 2 cut(s) 1106, 1203
SatI GCNGC 9 cut(s) 18, 757, 1493, 1567, 1871, 1879, 2158, 2494, 3239
Sau96I GGNCC 6 cut(s) 94, 145, 321, 1673, 2975, 3056
SchI GAGTC 6 cut(s) 418, 530, 2879, 2993, 3046, 3386
SduI GDGCHC 1 cut(s) 1101
SfcI CTRYAG 3 cut(s) 1493, 2857, 2968
Sfr274I CTCGAG 1 cut(s) 182
SinI GGWCC 3 cut(s) 145, 321, 2975
SlaI CTCGAG 1 cut(s) 182
SmaI CCCGGG 1 cut(s) 994
SmiMI CAYNNNNRTG 3 cut(s) 1655, 1789, 3852
SmlI CTYRAG 9 cut(s) 82, 182, 212, 580, 615, 1882, 2772, 3346, 3677
SmoI CTYRAG 9 cut(s) 82, 182, 212, 580, 615, 1882, 2772, 3346, 3677
SpeI ACTAGT 1 cut(s) 3902
SphI GCATGC 2 cut(s) 976, 1115
SseBI AGGCCT 1 cut(s) 1851
SsiI CCGC 3 cut(s) 1179, 3609, 3699
SspI AATATT 4 cut(s) 772, 835, 2369, 2409
SspMI CTAG 9 cut(s) 167, 1017, 1541, 1991, 2259, 2639, 3545, 3572, 3903
SstI GAGCTC 1 cut(s) 1101
StuI AGGCCT 1 cut(s) 1851
StyI CCWWGG 4 cut(s) 148, 2375, 3067, 3795
TaiI ACGT 6 cut(s) 360, 2724, 2964, 3553, 3642, 3712
TaqI TCGA 3 cut(s) 183, 1407, 2624
TaqII GACCGA 1 cut(s) 3426
TatI WGTACW 2 cut(s) 1747, 3166
TscAI CASTG 8 cut(s) 871, 877, 1288, 1766, 1972, 2571, 3300, 3457
TseI GCWGC 9 cut(s) 17, 756, 1492, 1566, 1870, 1878, 2157, 2493, 3238
TspGWI ACGGA 4 cut(s) 514, 1425, 1914, 2244
TspMI CCCGGG 1 cut(s) 992
TspRI CASTG 8 cut(s) 871, 877, 1288, 1766, 1972, 2571, 3300, 3457
Van91I CCANNNNNTGG 1 cut(s) 3710
Vha464I CTTAAG 1 cut(s) 580
VpaK11BI GGWCC 3 cut(s) 145, 321, 2975
XagI CCTNNNNNAGG 1 cut(s) 1137
XapI RAATTY 7 cut(s) 915, 1772, 2232, 2381, 2413, 2801, 3204
XbaI TCTAGA 1 cut(s) 1990
XceI RCATGY 3 cut(s) 976, 1115, 1654
XcmI CCANNNNNNNNNTGG 2 cut(s) 2042, 2050
XhoI CTCGAG 1 cut(s) 182
XmaI CCCGGG 1 cut(s) 992
XmiI GTMKAC 2 cut(s) 667, 2964
XmnI GAANNNNTTC 5 cut(s) 610, 2290, 2370, 2933, 3150
XspI CTAG 9 cut(s) 167, 1017, 1541, 1991, 2259, 2639, 3545, 3572, 3903
Zsp2I ATGCAT 5 cut(s) 1117, 1503, 1656, 1705, 1719
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.