Rw1G020530

SNARE-complex protein Syntaxin-18 N-terminus

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0006035
Physical Location & Seq
Forward (+)
44167060 .. 44170011
2952 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G020530.1

Sequence Viewer

Length: 933 bp
ATGGCCAAATTCAGAGACAGGATGGAAGATTTCAAAGACGCTGTGCGCCAAGCTGCCATTTCTTCCGGCTACAACGAGTCCAAATTGGCAGCGACTATGGCCAAGTTCATTATACATGCACCTCGCCAAAGGTCACCCTTCACCAGAGCTGCTTACAAAACGCTTGAAAGCATTGGAGCTCTGGAACAGTTTATGTTGAAACATCGAAAGGATTATGTGGATCGGCATCGCACCACTGAACATGAGAGAGATAGCATTGAGCAGGAAGTTAATGCTTTTATTAAAGCGTGCCAAGAACAAATTAATGTTCTCAAAAATAGCATTAATGATGAGGAGGCACAGTCAAAGGGCTGGCTTGGTATTAGGGCTGATCACTCTAATGCTGATACTATAGCACACAAGCATGGGGTGGTTTTGATTTTAAGTGAGAGACTTCATTCTGTCACGTCACATTATGATCAGCTAAGATCCATACGCTTCCAAGATACCTTTAGCAGAGCAACACCAAGAAGAAAACTTAACCGGAATCCAAAATCAAAATCCGAAGATACCTCCAATTCTAATAATTTAGAGGTCAGAGAACAACCTGAAGCATTTCAGGCGCAGTCTCTAACCGTCCAACAACAGCTCTTGGATGATGAAACACGCGCCCTTCAGGTAGAGTTGACTAGCCTTCTAGATGCAGTTCAGGAAACTGAAACTAAGATGGTGGAAATGTCTGCACTAAATCACCTTCTGTCTACACATGTTCTGCAACAACGTGAACAAATAGAGCAACTATATGACCAAGCAGTTGAAGCCACACACAATGTAGATCTCGGCAACAAAGAGCTTTCCAAAGCAATCCAGAGAAATACCAGCAGCAGGACCTTTCTTTTGCTCTTCCTATTTGTACTTACTTTTTCCATTCTCTTTCTTGATTGGTATAGTTAA

Protein Analysis

310

Amino Acids

35.76

Weight (kDa)

6.47

Isoelectric Point (pI)

50.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Syntaxin-18_N PF10496 6 - 86 6.9e-17 SNARE-complex protein Syntaxin-18 N-terminus
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 740
AccII CGCG 1 cut(s) 648
AclWI GGATC 2 cut(s) 228, 462
AcoI YGGCCR 2 cut(s) 3, 99
AcsI RAATTY 1 cut(s) 8
AcuI CTGAAG 2 cut(s) 609, 638
AfaI GTAC 1 cut(s) 894
AfiI CCNNNNNNNGG 1 cut(s) 864
AflIII ACRYGT 1 cut(s) 745
AgsI TTSAA 4 cut(s) 34, 167, 199, 797
AjiI CACGTC 1 cut(s) 447
AluBI AGCT 6 cut(s) 53, 149, 179, 463, 628, 832
AluI AGCT 6 cut(s) 53, 149, 179, 463, 628, 832
Alw21I GWGCWC 1 cut(s) 181
Alw26I GTCTC 3 cut(s) 9, 424, 612
AlwI GGATC 2 cut(s) 228, 462
AoxI GGCC 2 cut(s) 3, 99
ApeKI GCWGC 4 cut(s) 53, 89, 149, 861
ApoI RAATTY 1 cut(s) 8
ArsI GACNNNNNNTTYG 2 cut(s) 859, 891
AseI ATTAAT 2 cut(s) 303, 324
Asp700I GAANNNNTTC 1 cut(s) 594
AspLEI GCGC 3 cut(s) 48, 604, 650
AspS9I GGNCC 1 cut(s) 867
AsuHPI GGTGA 3 cut(s) 126, 133, 722
AvaII GGWCC 1 cut(s) 867
BalI TGGCCA 2 cut(s) 5, 101
BanII GRGCYC 1 cut(s) 181
Bbv12I GWGCWC 1 cut(s) 181
BbvI GCAGC 4 cut(s) 40, 101, 136, 873
BccI CCATC 2 cut(s) 16, 700
BclI TGATCA 2 cut(s) 370, 457
BcoDI GTCTC 3 cut(s) 9, 424, 612
BfaI CTAG 2 cut(s) 669, 677
BfmI CTRYAG 1 cut(s) 390
BglII AGATCT 1 cut(s) 814
BisI GCNGC 4 cut(s) 54, 90, 150, 862
BlsI GCNGC 4 cut(s) 55, 91, 151, 863
Bme18I GGWCC 1 cut(s) 867
BmgBI CACGTC 1 cut(s) 447
BmgT120I GGNCC 1 cut(s) 867
BmsI GCATC 2 cut(s) 235, 670
BsaBI GATNNNNATC 1 cut(s) 225
BsaWI WCCGGW 1 cut(s) 522
BsaXI ACNNNNNCTCC 2 cut(s) 326, 356
Bsc4I CCNNNNNNNGG 1 cut(s) 864
Bse8I GATNNNNATC 1 cut(s) 225
BseGI GGATG 2 cut(s) 27, 640
BseJI GATNNNNATC 1 cut(s) 225
BseLI CCNNNNNNNGG 1 cut(s) 864
BseRI GAGGAG 1 cut(s) 347
BseXI GCAGC 4 cut(s) 40, 101, 136, 873
BsgI GTGCAG 1 cut(s) 705
Bsh1236I CGCG 1 cut(s) 648
BshFI GGCC 2 cut(s) 5, 101
BsiHKAI GWGCWC 1 cut(s) 181
BsiSI CCGG 2 cut(s) 66, 523
BslI CCNNNNNNNGG 1 cut(s) 864
BsmAI GTCTC 3 cut(s) 9, 424, 612
BsnI GGCC 2 cut(s) 5, 101
Bsp1286I GDGCHC 1 cut(s) 181
Bsp143I GATC 5 cut(s) 220, 370, 457, 467, 814
BspANI GGCC 2 cut(s) 5, 101
BspFNI CGCG 1 cut(s) 648
BspPI GGATC 2 cut(s) 228, 462
BspQI GCTCTTC 1 cut(s) 887
BssMI GATC 5 cut(s) 220, 370, 457, 467, 814
Bst4CI ACNGT 3 cut(s) 189, 342, 616
Bst6I CTCTTC 1 cut(s) 887
BstC8I GCNNGC 2 cut(s) 289, 353
BstDEI CTNAG 2 cut(s) 464, 702
BstEII GGTNACC 1 cut(s) 132
BstF5I GGATG 2 cut(s) 27, 640
BstFNI CGCG 1 cut(s) 648
BstHHI GCGC 3 cut(s) 48, 604, 650
BstKTI GATC 5 cut(s) 223, 373, 460, 470, 817
BstMAI GTCTC 3 cut(s) 9, 424, 612
BstMBI GATC 5 cut(s) 220, 370, 457, 467, 814
BstMWI GCNNNNNNNGC 3 cut(s) 98, 599, 797
BstNSI RCATGY 2 cut(s) 119, 749
BstPI GGTNACC 1 cut(s) 132
BstSFI CTRYAG 1 cut(s) 390
BstUI CGCG 1 cut(s) 648
BstV1I GCAGC 4 cut(s) 40, 101, 136, 873
BstX2I RGATCY 2 cut(s) 467, 814
BstYI RGATCY 2 cut(s) 467, 814
BsuRI GGCC 2 cut(s) 5, 101
BtgZI GCGATG 1 cut(s) 212
BtrI CACGTC 1 cut(s) 447
BtsCI GGATG 2 cut(s) 27, 640
BtsIMutI CAGTG 1 cut(s) 234
Cac8I GCNNGC 2 cut(s) 289, 353
CfoI GCGC 3 cut(s) 48, 604, 650
Cfr13I GGNCC 1 cut(s) 867
CseI GACGC 1 cut(s) 47
Csp6I GTAC 1 cut(s) 893
CviAII CATG 4 cut(s) 116, 242, 404, 746
CviQI GTAC 1 cut(s) 893
DdeI CTNAG 2 cut(s) 464, 702
DpnI GATC 5 cut(s) 222, 372, 459, 469, 816
DpnII GATC 5 cut(s) 220, 370, 457, 467, 814
EaeI YGGCCR 2 cut(s) 3, 99
Eam1104I CTCTTC 1 cut(s) 887
EarI CTCTTC 1 cut(s) 887
Ecl136II GAGCTC 1 cut(s) 179
Eco24I GRGCYC 1 cut(s) 181
Eco47I GGWCC 1 cut(s) 867
Eco53kI GAGCTC 1 cut(s) 179
Eco57I CTGAAG 2 cut(s) 609, 638
Eco91I GGTNACC 1 cut(s) 132
EcoICRI GAGCTC 1 cut(s) 179
EcoO109I RGGNCCY 1 cut(s) 867
EcoO65I GGTNACC 1 cut(s) 132
EcoT38I GRGCYC 1 cut(s) 181
FaeI CATG 4 cut(s) 119, 245, 407, 749
FatI CATG 4 cut(s) 115, 241, 403, 745
FbaI TGATCA 2 cut(s) 370, 457
FblI GTMKAC 1 cut(s) 740
Fnu4HI GCNGC 4 cut(s) 54, 90, 150, 862
FokI GGATG 2 cut(s) 34, 647
FriOI GRGCYC 1 cut(s) 181
Fsp4HI GCNGC 4 cut(s) 54, 90, 150, 862
FspBI CTAG 2 cut(s) 669, 677
GlaI GCGC 3 cut(s) 47, 603, 649
GluI GCNGC 4 cut(s) 54, 90, 150, 862
HaeIII GGCC 2 cut(s) 5, 101
HapII CCGG 2 cut(s) 66, 523
HgaI GACGC 1 cut(s) 47
HhaI GCGC 3 cut(s) 48, 604, 650
Hin1II CATG 4 cut(s) 119, 245, 407, 749
Hin6I GCGC 3 cut(s) 46, 602, 648
HinP1I GCGC 3 cut(s) 46, 602, 648
HincII GTYRAC 1 cut(s) 666
HindII GTYRAC 1 cut(s) 666
HinfI GANTC 2 cut(s) 77, 526
HpaII CCGG 2 cut(s) 66, 523
HphI GGTGA 3 cut(s) 126, 133, 722
Hpy166II GTNNAC 3 cut(s) 666, 741, 764
Hpy188I TCNGA 3 cut(s) 14, 544, 578
Hpy188III TCNNGA 5 cut(s) 182, 677, 689, 847, 917
Hpy8I GTNNAC 3 cut(s) 666, 741, 764
HpyAV CCTTC 4 cut(s) 148, 662, 683, 743
HpyCH4III ACNGT 3 cut(s) 189, 342, 616
HpyCH4IV ACGT 2 cut(s) 446, 760
HpyCH4V TGCA 4 cut(s) 119, 683, 722, 754
HpyF10VI GCNNNNNNNGC 3 cut(s) 98, 599, 797
HpyF3I CTNAG 2 cut(s) 464, 702
HpySE526I ACGT 2 cut(s) 446, 760
Hsp92II CATG 4 cut(s) 119, 245, 407, 749
HspAI GCGC 3 cut(s) 46, 602, 648
Ksp22I TGATCA 2 cut(s) 370, 457
Kzo9I GATC 5 cut(s) 220, 370, 457, 467, 814
LguI GCTCTTC 1 cut(s) 887
LmnI GCTCC 1 cut(s) 176
Lsp1109I GCAGC 4 cut(s) 40, 101, 136, 873
LweI GCATC 2 cut(s) 235, 670
MaeI CTAG 2 cut(s) 669, 677
MaeII ACGT 2 cut(s) 446, 760
MaeIII GTNAC 3 cut(s) 132, 442, 447
MalI GATC 5 cut(s) 222, 372, 459, 469, 816
MboI GATC 5 cut(s) 220, 370, 457, 467, 814
MboII GAAGA 5 cut(s) 38, 54, 522, 557, 874
MflI RGATCY 2 cut(s) 467, 814
MhlI GDGCHC 1 cut(s) 181
MlsI TGGCCA 2 cut(s) 5, 101
MluCI AATT 5 cut(s) 8, 83, 300, 556, 565
MluNI TGGCCA 2 cut(s) 5, 101
MlyI GAGTC 1 cut(s) 86
MmeI TCCRAC 1 cut(s) 643
MnlI CCTC 5 cut(s) 132, 325, 328, 562, 565
Mox20I TGGCCA 2 cut(s) 5, 101
MroXI GAANNNNTTC 1 cut(s) 594
MscI TGGCCA 2 cut(s) 5, 101
MseI TTAA 7 cut(s) 270, 282, 303, 324, 422, 519, 931
MslI CAYNNNNRTG 2 cut(s) 378, 402
Msp20I TGGCCA 2 cut(s) 5, 101
MspI CCGG 2 cut(s) 66, 523
MvnI CGCG 1 cut(s) 648
MwoI GCNNNNNNNGC 3 cut(s) 98, 599, 797
NdeII GATC 5 cut(s) 220, 370, 457, 467, 814
NlaIII CATG 4 cut(s) 119, 245, 407, 749
NmeAIII GCCGAG 1 cut(s) 798
NmuCI GTSAC 3 cut(s) 132, 442, 447
NspI RCATGY 2 cut(s) 119, 749
PciI ACATGT 1 cut(s) 745
PciSI GCTCTTC 1 cut(s) 887
PdmI GAANNNNTTC 1 cut(s) 594
PfeI GAWTC 1 cut(s) 526
PkrI GCNGC 4 cut(s) 55, 91, 151, 863
PleI GAGTC 1 cut(s) 85
PpsI GAGTC 1 cut(s) 85
PpuMI RGGWCCY 1 cut(s) 867
PscI ACATGT 1 cut(s) 745
PshBI ATTAAT 2 cut(s) 303, 324
Psp124BI GAGCTC 1 cut(s) 181
Psp5II RGGWCCY 1 cut(s) 867
PspEI GGTNACC 1 cut(s) 132
PspPI GGNCC 1 cut(s) 867
PspPPI RGGWCCY 1 cut(s) 867
PsuI RGATCY 2 cut(s) 467, 814
RsaI GTAC 1 cut(s) 894
RsaNI GTAC 1 cut(s) 893
RseI CAYNNNNRTG 2 cut(s) 378, 402
SacI GAGCTC 1 cut(s) 181
SapI GCTCTTC 1 cut(s) 887
SaqAI TTAA 7 cut(s) 270, 282, 303, 324, 422, 519, 931
SatI GCNGC 4 cut(s) 54, 90, 150, 862
Sau3AI GATC 5 cut(s) 220, 370, 457, 467, 814
Sau96I GGNCC 1 cut(s) 867
SchI GAGTC 1 cut(s) 86
SduI GDGCHC 1 cut(s) 181
SfaNI GCATC 2 cut(s) 235, 670
SfcI CTRYAG 1 cut(s) 390
SinI GGWCC 1 cut(s) 867
SmiMI CAYNNNNRTG 2 cut(s) 378, 402
Sse9I AATT 5 cut(s) 8, 83, 300, 556, 565
SspMI CTAG 2 cut(s) 669, 677
SstI GAGCTC 1 cut(s) 181
TaaI ACNGT 3 cut(s) 189, 342, 616
TaiI ACGT 2 cut(s) 449, 763
TaqI TCGA 1 cut(s) 205
TasI AATT 5 cut(s) 8, 83, 300, 556, 565
TatI WGTACW 1 cut(s) 892
TfiI GAWTC 1 cut(s) 526
Tru1I TTAA 7 cut(s) 270, 282, 303, 324, 422, 519, 931
Tru9I TTAA 7 cut(s) 270, 282, 303, 324, 422, 519, 931
TscAI CASTG 1 cut(s) 241
TseFI GTSAC 3 cut(s) 132, 442, 447
TseI GCWGC 4 cut(s) 53, 89, 149, 861
Tsp45I GTSAC 3 cut(s) 132, 442, 447
TspDTI ATGAA 3 cut(s) 97, 425, 654
TspRI CASTG 1 cut(s) 241
VpaK11BI GGWCC 1 cut(s) 867
VspI ATTAAT 2 cut(s) 303, 324
XapI RAATTY 1 cut(s) 8
XbaI TCTAGA 1 cut(s) 676
XceI RCATGY 2 cut(s) 119, 749
XmiI GTMKAC 1 cut(s) 740
XmnI GAANNNNTTC 1 cut(s) 594
XspI CTAG 2 cut(s) 669, 677
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.