Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr02

Orthogroup ID

OG0010253

Physical Location & Seq

Genomic Coordinates Reverse (-)

19297895 .. 19303002

Gene Structure 5108 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD02G1198200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2202 bp

ATGGCCGGCGGTGGAGGAGACGCGCCGCCTCTCCCGTCCTCTAACGGCGGCGAATTCCTCCTCTCCTTGCTCCAACAACAAAAGCCCAACCTCCACCACCACCAGTCCCCCTCCCCACAACAACAGCAACCGCCTCTAGTGCTCGATCCGGCGGTTGCTGCGGTGGGACCCACACTCCCGTTCCCGCATATGCCGCCGTGGGTATCATCCAACGGCCAAGATCATCACCCTCAACTCCCCAACCCTTCTTCCCTCTGGTCCACGCAGTCCCCTCCTCCTAATTTCAACTTCCTAGGGTTTCCCCAAAGCCCTTATCCCTCTTCCTCTCCCCCGAATCCCTTTCCCCAGTTCGCCGGAAACCAATTCCCGGGAAACCTCGAGGATTTGAGGAATCTAGTCGGCTTTCAGAGTCCAAACAATAATCCCCTCCAAAACCTTGCCCAGCTGAATCAGCATCAAGATCAGAAGCTCAAGTTTAGTTATTTGCCTGGCGACCTTGTCCAAAACCCCGAAGGTTCAGCAAATGCCAGCATTTCCTCTGAGGTTTCGAAGCTCAGCAATAGCTTCGACAGGAATCTCAATTTGAGTTCTAACAATTCTTCCACTTCCAATGAGTTCCGGCATGGGAATCACGATAATTTCGGTTCGAATTTCAATTCTCGGGAGCAGGTACAGCAAGGCGGAGGTGCAAGTGGAAGGGGAAAGCAGTTTCAAAGGAATACTCCGCCGCCAGGGTTTGGGAACAATGCGAGGGGAGGAGGGAGTTGGAATTCTGGGAATAGGAGGGAGTTTGAGCATAATGTGGATAGGGATAGGCAGAGTTCGAGTGAATTTGTGAGGAACAGGGATGTTTCTTTTGAAGATGAGAGGATGAGAAGATTGGCAAATGAAGATGGTCGAACTCGAGGCAATGGGGTTCGGGGATTAGGGTTCAATGCACAGCCTGATGAGCCTGGTCTGCCCACTGGCACCAATCTCCATTCGGCTTCTGCTTTGGAGATTGAAGAGTCGATGATGAATTTACAACATGGAGATAAGGCGAGAAAAGACGTTGAAGATGGAAGCCAACAGTTGGTTGATGATGACAGGGATAAAAAGAATGAGGCAAAGCAACACCATAATGCGCGTGAAAAGGAGTCAAGATCAGATAACAGAGGACAACACCTACTTAACCAAAGAATGAGAATCTTCAAAAGACAGATGCAATGTCGCATGGACATAGACAGGCTAAATGCTCCATTTCTTGCAATTTATGATTCCTTGATACCGACTGAGGAAGAGAAGGCTAAGCAGAAGCAATTGTTTACATTATTGGAGAGGTTAGTTACTAAAGAATGGCCTGAAGCTCGACTATACATCTACGGATCGTGTGGCAACTCATTTGGGGTTTCTAAAAGTGATATTGATCTCTGTCTTGCAATTGATGAGGCAAACATTAACAAGTCAGAGTTCTTATTGAGGTTGGCAGATATTTTGCAATCAGACAGTCTACAGAATGTGCAGGCGTTGACACGTGCTAGGGTTCCCATAGTAAAGCTTATGGATCCTGTCACTGGAATCTCCTGTGACATATGCATCAACAATGTTTTGGCTGTCATAAATACAAAGCTTCTTCGTGATTATGCAAAAATTGATGCAAGATTACGGCAATTGGCTTTTATTGTGAAACATTGGGCCAAGTCAAGAGGAGTCAATGAAACTTACCATGGAACTTTATCAAGCTATGCGTATGTTTTGATGTGCATTCATTTCTTACAACATCGCAGACCTGCTATTCTCCCCTGCTTACAGGGAATGCAAACAACGTACTCTGTCACTGTAGAGAACGTTGAATGTTCTTTCTTTGATCAAGTAGACAAACTAAGAGATTTTGGGTCCCATAACAAGGAACCTATTGCTAGACTCGTGTGGGGATTTTTCAATTACTGGGCATATAGCCATGATTATGCAAATTCCGTCATTTCTGTTCGTACGGGAAGCTTAATCAGCAAGCGGGAAAAGGACTGGACAAGGAGGGTTGGGAATGATCGGCATTTGATATGCATAGAAGACCCTTTCGAGATATCCCACGACCTGGGTCGAGTGGTGGACAAGTACAGCATAAAAGTTCTAAGGGAGGAGTTTGAGCGTGCAGCCGACATTATGCAGTACGATGCAAATCCCTGTGTGAAGCTCTTTGAGCCGTACGTCCCTGATGTTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003824 GO:0005488 GO:0006139 GO:0006378 GO:0006396 GO:0006397 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009893 GO:0009987 GO:0010467 GO:0010468 GO:0010604 GO:0010628 GO:0016070 GO:0016071 GO:0016073 GO:0016074 GO:0016180 GO:0016740 GO:0016772 GO:0016779 GO:0019219 GO:0019222 GO:0031123 GO:0031124 GO:0031126 GO:0031323 GO:0031325 GO:0034470 GO:0034641 GO:0034660 GO:0043144 GO:0043170 GO:0043628 GO:0043629 GO:0043631 GO:0044237 GO:0044238 GO:0045935 GO:0046483 GO:0048518 GO:0048522 GO:0048583 GO:0048584 GO:0050265 GO:0050789 GO:0050794 GO:0051171 GO:0051173 GO:0051252 GO:0051254 GO:0060147 GO:0060148 GO:0060255 GO:0060964 GO:0060966 GO:0060968 GO:0065007 GO:0070569 GO:0070920 GO:0071050 GO:0071076 GO:0071704 GO:0080090 GO:0090065 GO:0090304 GO:0097159 GO:0140098 GO:1900368 GO:1900370 GO:1901360 GO:1901363 GO:1903705

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

NTP_transf_2 PAP_assoc

Protein Analysis

734

Amino Acids

82.53

Weight (kDa)

6.55

Isoelectric Point (pI)

51.28

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MTPAP-like_central	PF22600	415 - 542	7.2e-32	Poly(A) RNA polymerase, mitochondrial-like, central palm domain
TUTase	PF19088	424 - 524	1.8e-09	TUTase nucleotidyltransferase domain
PAP_assoc	PF03828	632 - 690	2.4e-19	Cid1 family poly A polymerase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010253)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G45620
fragaria_vesca	FvH4_7g12540 FvH4_7g12540 FvH4_7g12540 FvH4_7g12540 FvH4_7g12540
malus_domestica	MD02G1198200.v1.1
prunus_persica	Prupe.2G149800_v2.0.a1 Prupe.2G149800_v2.0.a1
pyrus_communis	pycom02g16180
rosa_chinensis	RchiOBHm_Chr1g0351451
rosa_laevigata	RLG00000028407
rosa_multiflora	Rmu_ssc0000403.1_g000022
rosa_roxburghii	Rroxscaffold_4G00303810
rosa_rugosa	Rorug01G0218100 Rorug01G0218200
rosa_samantha	Rh1AG232700 Rh1BG203700 Rh1CG217400 Rh1DG230600
rosa_wichuraiana	Rw1G020260

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AbsI	CCTCGAGG	1 cut(s)	377
Acc36I	ACCTGC	2 cut(s)	658, 1777
AccB1I	GGYRCC	1 cut(s)	968
AccB7I	CCANNNNNTGG	3 cut(s)	737, 1072, 2074
AccI	GTMKAC	2 cut(s)	1489, 1854
AccII	CGCG	2 cut(s)	23, 1126
AclI	AACGTT	1 cut(s)	1827
AclWI	GGATC	4 cut(s)	140, 1372, 1538, 1551
AcoI	YGGCCR	2 cut(s)	3, 214
AcsI	RAATTY	6 cut(s)	53, 649, 769, 830, 1018, 1951
AcuI	CTGAAG	1 cut(s)	1362
AcvI	CACGTG	1 cut(s)	1514
AfaI	GTAC	6 cut(s)	672, 1808, 1972, 2096, 2150, 2186
AfiI	CCNNNNNNNGG	7 cut(s)	367, 731, 737, 1072, 1553, 1885, 2074
AflIII	ACRYGT	1 cut(s)	1511
AjnI	CCWGG	4 cut(s)	487, 730, 952, 2073
Alw21I	GWGCWC	1 cut(s)	144
Alw26I	GTCTC	1 cut(s)	12
AlwI	GGATC	4 cut(s)	140, 1372, 1538, 1551
Ama87I	CYCGRG	4 cut(s)	367, 377, 660, 903
AoxI	GGCC	4 cut(s)	3, 214, 1337, 1674
ApeKI	GCWGC	2 cut(s)	158, 2132
ApoI	RAATTY	6 cut(s)	53, 649, 769, 830, 1018, 1951
AspA2I	CCTAGG	1 cut(s)	292
AspLEI	GCGC	2 cut(s)	25, 1126
AspS9I	GGNCC	4 cut(s)	167, 258, 1674, 1875
AsuC2I	CCSGG	2 cut(s)	368, 369
AsuHPI	GGTGA	1 cut(s)	218
AsuII	TTCGAA	2 cut(s)	548, 647
AvaI	CYCGRG	4 cut(s)	367, 377, 660, 903
AvaII	GGWCC	3 cut(s)	167, 258, 1875
AvrII	CCTAGG	1 cut(s)	292
BamHI	GGATCC	1 cut(s)	1543
BanI	GGYRCC	1 cut(s)	968
BauI	CACGAG	1 cut(s)	1904
BbrPI	CACGTG	1 cut(s)	1514
BbsI	GAAGAC	1 cut(s)	2055
Bbv12I	GWGCWC	1 cut(s)	144
BbvI	GCAGC	2 cut(s)	145, 2144
BccI	CCATC	2 cut(s)	887, 1052
BceAI	ACGGC	5 cut(s)	61, 181, 229, 1661, 2167
BcgI	CGANNNNNNTGC	2 cut(s)	547, 581
BciT130I	CCWGG	4 cut(s)	489, 732, 954, 2075
BclI	TGATCA	1 cut(s)	1846
BcnI	CCSGG	2 cut(s)	368, 369
BcoDI	GTCTC	1 cut(s)	12
BfaI	CTAG	5 cut(s)	137, 293, 395, 1518, 1899
BfmI	CTRYAG	2 cut(s)	1490, 1818
BfuAI	ACCTGC	2 cut(s)	658, 1777
BisI	GCNGC	6 cut(s)	26, 49, 159, 194, 728, 2133
BlnI	CCTAGG	1 cut(s)	292
BlpI	GCTNAGC	2 cut(s)	554, 1287
BlsI	GCNGC	6 cut(s)	27, 50, 160, 195, 729, 2134
Bme1390I	CCNGG	6 cut(s)	368, 369, 489, 732, 954, 2075
Bme18I	GGWCC	3 cut(s)	167, 258, 1875
BmeT110I	CYCGRG	4 cut(s)	367, 377, 660, 903
BmgT120I	GGNCC	4 cut(s)	167, 258, 1674, 1875
BmiI	GGNNCC	8 cut(s)	168, 169, 970, 1524, 1545, 1876, 1877, 1890
BmrFI	CCNGG	6 cut(s)	368, 369, 489, 732, 954, 2075
BmrI	ACTGGG	2 cut(s)	340, 1936
BmsI	GCATC	5 cut(s)	463, 1191, 1584, 1624, 2143
BmuI	ACTGGG	2 cut(s)	340, 1936
BoxI	GACNNNNGTC	1 cut(s)	2076
BpiI	GAAGAC	1 cut(s)	2055
Bpu1102I	GCTNAGC	2 cut(s)	554, 1287
Bpu14I	TTCGAA	2 cut(s)	548, 647
BpuEI	CTTGAG	1 cut(s)	455
BpuMI	CCSGG	2 cut(s)	368, 369
BsaAI	YACGTR	1 cut(s)	1514
BsaBI	GATNNNNATC	2 cut(s)	1404, 2157
BsaJI	CCNNGG	6 cut(s)	197, 292, 367, 731, 1705, 2074
BsaXI	ACNNNNNCTCC	2 cut(s)	159, 189
Bsc4I	CCNNNNNNNGG	7 cut(s)	367, 731, 737, 1072, 1553, 1885, 2074
Bse118I	RCCGGY	1 cut(s)	5
Bse1I	ACTGG	6 cut(s)	103, 346, 970, 1558, 1931, 2009
Bse3DI	GCAATG	2 cut(s)	916, 1211
Bse8I	GATNNNNATC	2 cut(s)	1404, 2157
BseBI	CCWGG	4 cut(s)	489, 732, 954, 2075
BseDI	CCNNGG	6 cut(s)	197, 292, 367, 731, 1705, 2074
BseGI	GGATG	3 cut(s)	206, 853, 876
BseJI	GATNNNNATC	2 cut(s)	1404, 2157
BseLI	CCNNNNNNNGG	7 cut(s)	367, 731, 737, 1072, 1553, 1885, 2074
BseMI	GCAATG	2 cut(s)	916, 1211
BseMII	CTCAG	3 cut(s)	531, 568, 1263
BseNI	ACTGG	6 cut(s)	103, 346, 970, 1558, 1931, 2009
BseRI	GAGGAG	6 cut(s)	30, 50, 264, 771, 1701, 2132
BseXI	GCAGC	2 cut(s)	145, 2144
BseYI	CCCAGC	1 cut(s)	441
BsgI	GTGCAG	2 cut(s)	1520, 2151
Bsh1236I	CGCG	2 cut(s)	23, 1126
BshFI	GGCC	4 cut(s)	5, 216, 1339, 1676
BshNI	GGYRCC	1 cut(s)	968
BsiHKAI	GWGCWC	1 cut(s)	144
BsiHKCI	CYCGRG	4 cut(s)	367, 377, 660, 903
BsiSI	CCGG	5 cut(s)	6, 149, 354, 368, 619
BsiWI	CGTACG	2 cut(s)	1970, 2184
BslFI	GGGAC	5 cut(s)	91, 180, 253, 1861, 2174
BslI	CCNNNNNNNGG	7 cut(s)	367, 731, 737, 1072, 1553, 1885, 2074
BsmAI	GTCTC	1 cut(s)	12
BsmBI	CGTCTC	1 cut(s)	12
BsmFI	GGGAC	5 cut(s)	91, 180, 253, 1861, 2174
BsmI	GAATGC	2 cut(s)	1743, 1800
BsnI	GGCC	4 cut(s)	5, 216, 1339, 1676
BsoBI	CYCGRG	4 cut(s)	367, 377, 660, 903
Bsp119I	TTCGAA	2 cut(s)	548, 647
Bsp1286I	GDGCHC	1 cut(s)	144
Bsp143I	GATC	9 cut(s)	145, 220, 460, 1142, 1364, 1405, 1543, 1846, 2026
Bsp1720I	GCTNAGC	2 cut(s)	554, 1287
Bsp19I	CCATGG	1 cut(s)	1705
BspANI	GGCC	4 cut(s)	5, 216, 1339, 1676
BspCNI	CTCAG	3 cut(s)	532, 567, 1264
BspFNI	CGCG	2 cut(s)	23, 1126
BspLI	GGNNCC	8 cut(s)	168, 169, 970, 1524, 1545, 1876, 1877, 1890
BspMI	ACCTGC	2 cut(s)	658, 1777
BspPI	GGATC	4 cut(s)	140, 1372, 1538, 1551
BspT104I	TTCGAA	2 cut(s)	548, 647
BspT107I	GGYRCC	1 cut(s)	968
BsrDI	GCAATG	2 cut(s)	916, 1211
BsrFI	RCCGGY	1 cut(s)	5
BsrI	ACTGG	6 cut(s)	103, 346, 970, 1558, 1931, 2009
BssAI	RCCGGY	1 cut(s)	5
BssECI	CCNNGG	6 cut(s)	197, 292, 367, 731, 1705, 2074
BssMI	GATC	9 cut(s)	145, 220, 460, 1142, 1364, 1405, 1543, 1846, 2026
BssSI	CACGAG	1 cut(s)	1904
BssT1I	CCWWGG	2 cut(s)	292, 1705
Bst2BI	CACGAG	1 cut(s)	1904
Bst2UI	CCWGG	4 cut(s)	489, 732, 954, 2075
Bst4CI	ACNGT	3 cut(s)	1071, 1487, 1819
Bst6I	CTCTTC	3 cut(s)	325, 999, 1272
BstBAI	YACGTR	1 cut(s)	1514
BstBI	TTCGAA	2 cut(s)	548, 647
BstC8I	GCNNGC	5 cut(s)	7, 529, 1503, 1991, 2130
BstDEI	CTNAG	6 cut(s)	540, 554, 1272, 1287, 1862, 2111
BstDSI	CCRYGG	2 cut(s)	197, 1705
BstF5I	GGATG	3 cut(s)	206, 853, 876
BstFNI	CGCG	2 cut(s)	23, 1126
BstHHI	GCGC	2 cut(s)	25, 1126
BstKTI	GATC	9 cut(s)	148, 223, 463, 1145, 1367, 1408, 1546, 1849, 2029
BstMAI	GTCTC	1 cut(s)	12
BstMBI	GATC	9 cut(s)	145, 220, 460, 1142, 1364, 1405, 1543, 1846, 2026
BstMWI	GCNNNNNNNGC	9 cut(s)	139, 158, 193, 451, 673, 949, 958, 1986, 2179
BstNI	CCWGG	4 cut(s)	489, 732, 954, 2075
BstPAI	GACNNNNGTC	1 cut(s)	2076
BstSCI	CCNGG	6 cut(s)	366, 367, 487, 730, 952, 2073
BstSFI	CTRYAG	2 cut(s)	1490, 1818
BstUI	CGCG	2 cut(s)	23, 1126
BstV1I	GCAGC	2 cut(s)	145, 2144
BstV2I	GAAGAC	1 cut(s)	2055
BstX2I	RGATCY	1 cut(s)	1543
BstYI	RGATCY	1 cut(s)	1543
BsuRI	GGCC	4 cut(s)	5, 216, 1339, 1676
BtgI	CCRYGG	2 cut(s)	197, 1705
BtgZI	GCGATG	1 cut(s)	1745
BtsCI	GGATG	3 cut(s)	206, 853, 876
BtsIMutI	CAGTG	3 cut(s)	963, 1551, 1815
BveI	ACCTGC	2 cut(s)	658, 1777
Cac8I	GCNNGC	5 cut(s)	7, 529, 1503, 1991, 2130
CfoI	GCGC	2 cut(s)	25, 1126
Cfr10I	RCCGGY	1 cut(s)	5
Cfr13I	GGNCC	4 cut(s)	167, 258, 1674, 1875
Cfr9I	CCCGGG	1 cut(s)	367
CseI	GACGC	1 cut(s)	29
Csp6I	GTAC	6 cut(s)	671, 1807, 1971, 2095, 2149, 2185
CviAII	CATG	5 cut(s)	623, 1028, 1213, 1706, 1940
CviQI	GTAC	6 cut(s)	671, 1807, 1971, 2095, 2149, 2185
DdeI	CTNAG	6 cut(s)	540, 554, 1272, 1287, 1862, 2111
DpnI	GATC	9 cut(s)	147, 222, 462, 1144, 1366, 1407, 1545, 1848, 2028
DpnII	GATC	9 cut(s)	145, 220, 460, 1142, 1364, 1405, 1543, 1846, 2026
EaeI	YGGCCR	2 cut(s)	3, 214
Eam1104I	CTCTTC	3 cut(s)	325, 999, 1272
EarI	CTCTTC	3 cut(s)	325, 999, 1272
EciI	GGCGGA	2 cut(s)	696, 714
Eco130I	CCWWGG	2 cut(s)	292, 1705
Eco32I	GATATC	1 cut(s)	2064
Eco47I	GGWCC	3 cut(s)	167, 258, 1875
Eco57I	CTGAAG	1 cut(s)	1362
Eco72I	CACGTG	1 cut(s)	1514
Eco88I	CYCGRG	4 cut(s)	367, 377, 660, 903
EcoO109I	RGGNCCY	2 cut(s)	167, 1875
EcoRI	GAATTC	2 cut(s)	53, 769
EcoRII	CCWGG	4 cut(s)	487, 730, 952, 2073
EcoRV	GATATC	1 cut(s)	2064
EcoT14I	CCWWGG	2 cut(s)	292, 1705
EcoT22I	ATGCAT	2 cut(s)	1577, 2045
ErhI	CCWWGG	2 cut(s)	292, 1705
Esp3I	CGTCTC	1 cut(s)	12
FaeI	CATG	5 cut(s)	626, 1031, 1216, 1709, 1943
FaqI	GGGAC	5 cut(s)	91, 180, 253, 1861, 2174
FatI	CATG	5 cut(s)	622, 1027, 1212, 1705, 1939
FauI	CCCGC	2 cut(s)	192, 1986
FauNDI	CATATG	2 cut(s)	189, 1571
FbaI	TGATCA	1 cut(s)	1846
FblI	GTMKAC	2 cut(s)	1489, 1854
Fnu4HI	GCNGC	6 cut(s)	26, 49, 159, 194, 728, 2133
FokI	GGATG	3 cut(s)	193, 860, 883
Fsp4HI	GCNGC	6 cut(s)	26, 49, 159, 194, 728, 2133
FspBI	CTAG	5 cut(s)	137, 293, 395, 1518, 1899
GlaI	GCGC	2 cut(s)	24, 1125
GluI	GCNGC	6 cut(s)	26, 49, 159, 194, 728, 2133
GsaI	CCCAGC	1 cut(s)	445
HaeIII	GGCC	4 cut(s)	5, 216, 1339, 1676
HapII	CCGG	5 cut(s)	6, 149, 354, 368, 619
HgaI	GACGC	1 cut(s)	29
HhaI	GCGC	2 cut(s)	25, 1126
Hin1II	CATG	5 cut(s)	626, 1031, 1216, 1709, 1943
Hin6I	GCGC	2 cut(s)	23, 1124
HinP1I	GCGC	2 cut(s)	23, 1124
HincII	GTYRAC	1 cut(s)	1509
HindII	GTYRAC	1 cut(s)	1509
HindIII	AAGCTT	3 cut(s)	1535, 1607, 1978
HpaII	CCGG	5 cut(s)	6, 149, 354, 368, 619
HphI	GGTGA	1 cut(s)	218
Hpy166II	GTNNAC	6 cut(s)	261, 1305, 1490, 1509, 1855, 2089
Hpy188I	TCNGA	6 cut(s)	408, 465, 541, 1147, 1447, 1483
Hpy188III	TCNNGA	7 cut(s)	458, 632, 662, 1140, 1616, 1683, 2059
Hpy8I	GTNNAC	6 cut(s)	261, 1305, 1490, 1509, 1855, 2089
HpyAV	CCTTC	4 cut(s)	255, 506, 690, 1276
HpyCH4III	ACNGT	3 cut(s)	1071, 1487, 1819
HpyCH4IV	ACGT	5 cut(s)	1050, 1513, 1805, 1827, 2187
HpyF10VI	GCNNNNNNNGC	9 cut(s)	139, 158, 193, 451, 673, 949, 958, 1986, 2179
HpyF3I	CTNAG	6 cut(s)	540, 554, 1272, 1287, 1862, 2111
HpySE526I	ACGT	5 cut(s)	1050, 1513, 1805, 1827, 2187
Hsp92II	CATG	5 cut(s)	626, 1031, 1216, 1709, 1943
HspAI	GCGC	2 cut(s)	23, 1124
KflI	GGGWCCC	2 cut(s)	167, 1875
KroI	GCCGGC	1 cut(s)	5
KroNI	GCCGGC	1 cut(s)	7
Ksp22I	TGATCA	1 cut(s)	1846
Kzo9I	GATC	9 cut(s)	145, 220, 460, 1142, 1364, 1405, 1543, 1846, 2026
LmnI	GCTCC	3 cut(s)	75, 664, 1240
Lsp1109I	GCAGC	2 cut(s)	145, 2144
LweI	GCATC	5 cut(s)	463, 1191, 1584, 1624, 2143
MaeI	CTAG	5 cut(s)	137, 293, 395, 1518, 1899
MaeII	ACGT	5 cut(s)	1050, 1513, 1805, 1827, 2187
MaeIII	GTNAC	4 cut(s)	1324, 1549, 1565, 1813
MalI	GATC	9 cut(s)	147, 222, 462, 1144, 1366, 1407, 1545, 1848, 2028
MboI	GATC	9 cut(s)	145, 220, 460, 1142, 1364, 1405, 1543, 1846, 2026
MfeI	CAATTG	3 cut(s)	1298, 1419, 1649
MflI	RGATCY	1 cut(s)	1543
MhlI	GDGCHC	1 cut(s)	144
MlyI	GAGTC	5 cut(s)	418, 1016, 1145, 1698, 1896
MmeI	TCCRAC	3 cut(s)	97, 234, 746
Mph1103I	ATGCAT	2 cut(s)	1577, 2045
MroNI	GCCGGC	1 cut(s)	5
MseI	TTAA	4 cut(s)	1170, 1437, 1982, 2200
MslI	CAYNNNNRTG	2 cut(s)	1119, 1944
MspA1I	CMGCKG	1 cut(s)	445
MspI	CCGG	5 cut(s)	6, 149, 354, 368, 619
MspR9I	CCNGG	6 cut(s)	368, 369, 489, 732, 954, 2075
MunI	CAATTG	3 cut(s)	1298, 1419, 1649
Mva1269I	GAATGC	2 cut(s)	1743, 1800
MvaI	CCWGG	4 cut(s)	489, 732, 954, 2075
MvnI	CGCG	2 cut(s)	23, 1126
MwoI	GCNNNNNNNGC	9 cut(s)	139, 158, 193, 451, 673, 949, 958, 1986, 2179
NaeI	GCCGGC	1 cut(s)	7
NciI	CCSGG	2 cut(s)	368, 369
NcoI	CCATGG	1 cut(s)	1705
NdeI	CATATG	2 cut(s)	189, 1571
NdeII	GATC	9 cut(s)	145, 220, 460, 1142, 1364, 1405, 1543, 1846, 2026
NgoMIV	GCCGGC	1 cut(s)	5
NlaIII	CATG	5 cut(s)	626, 1031, 1216, 1709, 1943
NlaIV	GGNNCC	8 cut(s)	168, 169, 970, 1524, 1545, 1876, 1877, 1890
NmuCI	GTSAC	3 cut(s)	1549, 1565, 1813
NsiI	ATGCAT	2 cut(s)	1577, 2045
NspV	TTCGAA	2 cut(s)	548, 647
PaeR7I	CTCGAG	2 cut(s)	377, 903
PctI	GAATGC	2 cut(s)	1743, 1800
PdiI	GCCGGC	1 cut(s)	7
PfeI	GAWTC	8 cut(s)	334, 391, 448, 574, 628, 1185, 1256, 1557
Pfl23II	CGTACG	2 cut(s)	1970, 2184
PflFI	GACNNNGTC	1 cut(s)	497
PflMI	CCANNNNNTGG	3 cut(s)	737, 1072, 2074
PkrI	GCNGC	6 cut(s)	27, 50, 160, 195, 729, 2134
PleI	GAGTC	5 cut(s)	417, 1015, 1144, 1697, 1896
PmaCI	CACGTG	1 cut(s)	1514
PmlI	CACGTG	1 cut(s)	1514
PpsI	GAGTC	5 cut(s)	417, 1015, 1144, 1697, 1896
Ppu21I	YACGTR	1 cut(s)	1514
PpuMI	RGGWCCY	2 cut(s)	167, 1875
PshAI	GACNNNNGTC	1 cut(s)	2076
Psp1406I	AACGTT	1 cut(s)	1827
Psp5II	RGGWCCY	2 cut(s)	167, 1875
Psp6I	CCWGG	4 cut(s)	487, 730, 952, 2073
PspCI	CACGTG	1 cut(s)	1514
PspFI	CCCAGC	1 cut(s)	441
PspGI	CCWGG	4 cut(s)	487, 730, 952, 2073
PspLI	CGTACG	2 cut(s)	1970, 2184
PspN4I	GGNNCC	8 cut(s)	168, 169, 970, 1524, 1545, 1876, 1877, 1890
PspPI	GGNCC	4 cut(s)	167, 258, 1674, 1875
PspPPI	RGGWCCY	2 cut(s)	167, 1875
PspXI	VCTCGAGB	2 cut(s)	377, 903
PsuI	RGATCY	1 cut(s)	1543
PsyI	GACNNNGTC	1 cut(s)	497
PvuII	CAGCTG	1 cut(s)	445
RsaI	GTAC	6 cut(s)	672, 1808, 1972, 2096, 2150, 2186
RsaNI	GTAC	6 cut(s)	671, 1807, 1971, 2095, 2149, 2185
RseI	CAYNNNNRTG	2 cut(s)	1119, 1944
SaqAI	TTAA	4 cut(s)	1170, 1437, 1982, 2200
SatI	GCNGC	6 cut(s)	26, 49, 159, 194, 728, 2133
Sau3AI	GATC	9 cut(s)	145, 220, 460, 1142, 1364, 1405, 1543, 1846, 2026
Sau96I	GGNCC	4 cut(s)	167, 258, 1674, 1875
SchI	GAGTC	5 cut(s)	418, 1016, 1145, 1698, 1896
ScrFI	CCNGG	6 cut(s)	368, 369, 489, 732, 954, 2075
SduI	GDGCHC	1 cut(s)	144
SfaNI	GCATC	5 cut(s)	463, 1191, 1584, 1624, 2143
SfcI	CTRYAG	2 cut(s)	1490, 1818
Sfr274I	CTCGAG	2 cut(s)	377, 903
SfuI	TTCGAA	2 cut(s)	548, 647
SinI	GGWCC	3 cut(s)	167, 258, 1875
SlaI	CTCGAG	2 cut(s)	377, 903
SmaI	CCCGGG	1 cut(s)	369
SmiMI	CAYNNNNRTG	2 cut(s)	1119, 1944
SmlI	CTYRAG	3 cut(s)	377, 470, 903
SmoI	CTYRAG	3 cut(s)	377, 470, 903
SspMI	CTAG	5 cut(s)	137, 293, 395, 1518, 1899
StyD4I	CCNGG	6 cut(s)	366, 367, 487, 730, 952, 2073
StyI	CCWWGG	2 cut(s)	292, 1705
TaaI	ACNGT	3 cut(s)	1071, 1487, 1819
TaiI	ACGT	5 cut(s)	1053, 1516, 1808, 1830, 2190
TatI	WGTACW	1 cut(s)	2094
TauI	GCSGC	4 cut(s)	28, 51, 196, 730
TfiI	GAWTC	8 cut(s)	334, 391, 448, 574, 628, 1185, 1256, 1557
Tru1I	TTAA	4 cut(s)	1170, 1437, 1982, 2200
Tru9I	TTAA	4 cut(s)	1170, 1437, 1982, 2200
TscAI	CASTG	3 cut(s)	970, 1558, 1822
TseFI	GTSAC	3 cut(s)	1549, 1565, 1813
TseI	GCWGC	2 cut(s)	158, 2132
Tsp45I	GTSAC	3 cut(s)	1549, 1565, 1813
TspDTI	ATGAA	4 cut(s)	903, 1031, 1710, 1736
TspGWI	ACGGA	2 cut(s)	1377, 1945
TspMI	CCCGGG	1 cut(s)	367
TspRI	CASTG	3 cut(s)	970, 1558, 1822
Tth111I	GACNNNGTC	1 cut(s)	497
Van91I	CCANNNNNTGG	3 cut(s)	737, 1072, 2074
VpaK11BI	GGWCC	3 cut(s)	167, 258, 1875
XapI	RAATTY	6 cut(s)	53, 649, 769, 830, 1018, 1951
XhoI	CTCGAG	2 cut(s)	377, 903
XmaI	CCCGGG	1 cut(s)	367
XmaJI	CCTAGG	1 cut(s)	292
XmiI	GTMKAC	2 cut(s)	1489, 1854
XspI	CTAG	5 cut(s)	137, 293, 395, 1518, 1899
Zsp2I	ATGCAT	2 cut(s)	1577, 2045

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.