MD02G1258300.v1.1

Glutaredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0016741
Physical Location & Seq
Reverse (-)
31160676 .. 31161821
1146 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1258300.v1.1.491

Sequence Viewer

Length: 1146 bp
ATGAAAGGGGTGAAAGGGAAGTTGATGATGAAGTTGAAGTCAATCGGACCGATTGGACCGATTGGAATTGGGTACCTGAAACCCCAAGATCGAGTTCTTCAGGTAAATGCATCGGATGGGTACGCGGATTCAAATCTCAACATTCAGACCCATAATTTGATTTCGGAAGGAACGAAGCAGAAGAAGAAGATCAAACAGTTAGAGAAGAATGTGACGGAGAAAGAGCCTGACATCATCGACGTCACCGAGCTTATGAGAGACCTCGAAGATGAAGAAGAGTCAGAGCAGGATGGTGATGATATCGATGTCGACGACAAGGAAAACGTTAGGCCTAAAATGGTGGCAAAACACCCTGTAGAGTGCAGTAAAAGTAGGGATAAAACGGAGTCAAATTTGGAGGAAAACAGAGCTTCTGAAGCTTCAGAGAATCGTAGACAGACCCCCTTGTCGGAGATTGACATCTCATCTTTTCGGAGACCGGACTTAAACTCCGGTAGCCTCTTCGACCCGGACCTACTAGCAGCCTTTCAGCAAGCAGTGATGGAATATATGAGGTTGAGCGAAGAAGAGAAAAATGCAAGGAGAAGCGAAAAAGAAGATATTGAAGCGCCAGATCCCGAGCCAGTGCCAGAGCTAGAGCCAACACTGCCTTCAAATGAAGACGCTCTATTAGGCTTTGAAGAAAAGTGTCCGCCTGGTAGTTTGGATTCATCGGTAGTTCTCTACACTACAACCCTTCGGGGGATTCGGAAGACTTTTGATGATTGCAACAGCGTTCGATTTCTTTTGGAGAGCTTTCGGGTGGTGTTCTACGAGCGAGATGTGTCAATGCACATGGAATTCAGACAAGAGCTGTGGAAGATTCTGGATTGCAAAGCAGTGCCTCCAAAGCTTTTCATAAAGGGGAGGTATATCGGGGGAGCGGAGGAGGTTTTAACGCTTCATGAACAAGGAAAGCTCAAGCCGCTCTTCGAAGGAGTCCCGTTGGATCGGTCCATTGGCCCCTGTGAAGCTTGTGATGGAGTTCGATTTGTGGTTTGCTTCAGATGCAGCGGGAGCTGCAAGCTCATCGCCGATGAGGGGCAGCCGGATAAGTGCCCCGAGTGTAACGAAAATGGGTTGATTATATGCCCCTTGTGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

382

Amino Acids

42.91

Weight (kDa)

4.82

Isoelectric Point (pI)

56.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 239 - 305 1.9e-11 Glutaredoxin
GRXCR1-2_C PF23733 336 - 381 5.1e-17 GRXCR1/2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016741)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g27780 FvH4_1g27790 FvH4_7g05400
malus_domestica MD02G1258300.v1.1 MD07G1062500.v1.1
prunus_persica Prupe.2G073600_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0332071
rosa_laevigata RLG00000029694
rosa_roxburghii Rroxscaffold_4G00318400
rosa_rugosa Rorug01G0098200
rosa_samantha Rh1AG122100 Rh1BG093500 Rh1CG116700
rosa_wichuraiana Rw1G009950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 445
AatII GACGTC 1 cut(s) 243
Acc65I GGTACC 1 cut(s) 72
AccB1I GGYRCC 1 cut(s) 72
AccBSI CCGCTC 2 cut(s) 923, 967
AccI GTMKAC 2 cut(s) 309, 433
AccII CGCG 1 cut(s) 125
AciI CCGC 5 cut(s) 125, 692, 923, 965, 1053
AclI AACGTT 1 cut(s) 324
AclWI GGATC 2 cut(s) 608, 996
AcsI RAATTY 2 cut(s) 391, 839
AcuI CTGAAG 4 cut(s) 83, 405, 435, 1027
AcyI GRCGYC 1 cut(s) 240
AfaI GTAC 2 cut(s) 74, 122
AfiI CCNNNNNNNGG 3 cut(s) 448, 741, 1080
AgsI TTSAA 5 cut(s) 37, 132, 605, 654, 680
AjnI CCWGG 1 cut(s) 694
AjuI GAANNNNNNNTTGG 4 cut(s) 78, 110, 634, 666
Alw26I GTCTC 2 cut(s) 252, 469
AlwI GGATC 2 cut(s) 608, 996
Ama87I CYCGRG 2 cut(s) 617, 1100
AoxI GGCC 2 cut(s) 329, 1000
ApeKI GCWGC 4 cut(s) 521, 1050, 1059, 1084
ApoI RAATTY 2 cut(s) 391, 839
ArsI GACNNNNNNTTYG 2 cut(s) 139, 171
Asp718I GGTACC 1 cut(s) 72
AspLEI GCGC 1 cut(s) 610
AspS9I GGNCC 5 cut(s) 47, 56, 511, 993, 1001
AsuC2I CCSGG 1 cut(s) 509
AsuHPI GGTGA 3 cut(s) 22, 235, 305
AsuII TTCGAA 1 cut(s) 972
AvaI CYCGRG 2 cut(s) 617, 1100
AvaII GGWCC 4 cut(s) 47, 56, 511, 993
BaeGI GKGCMC 1 cut(s) 1100
BanI GGYRCC 1 cut(s) 72
BbsI GAAGAC 2 cut(s) 666, 758
BbvI GCAGC 4 cut(s) 533, 1046, 1062, 1096
BccI CCATC 4 cut(s) 110, 284, 535, 1013
BcgI CGANNNNNNTGC 2 cut(s) 1051, 1085
BciT130I CCWGG 1 cut(s) 696
BcnI CCSGG 1 cut(s) 509
BcoDI GTCTC 2 cut(s) 252, 469
BfaI CTAG 2 cut(s) 518, 635
BfmI CTRYAG 1 cut(s) 354
BfoI RGCGCY 1 cut(s) 611
BisI GCNGC 5 cut(s) 522, 965, 1051, 1060, 1085
BlsI GCNGC 5 cut(s) 523, 966, 1052, 1061, 1086
Bme1390I CCNGG 2 cut(s) 509, 696
Bme18I GGWCC 4 cut(s) 47, 56, 511, 993
BmeT110I CYCGRG 2 cut(s) 617, 1100
BmgT120I GGNCC 5 cut(s) 47, 56, 511, 993, 1001
BmiI GGNNCC 2 cut(s) 74, 1003
BmrFI CCNGG 2 cut(s) 509, 696
BmsI GCATC 2 cut(s) 119, 1037
BpiI GAAGAC 2 cut(s) 666, 758
Bpu14I TTCGAA 1 cut(s) 972
BpuEI CTTGAG 1 cut(s) 944
BpuMI CCSGG 1 cut(s) 509
Bsa29I ATCGAT 1 cut(s) 303
BsaBI GATNNNNATC 2 cut(s) 132, 458
BsaHI GRCGYC 1 cut(s) 240
BsaI GGTCTC 2 cut(s) 252, 469
BsaWI WCCGGW 2 cut(s) 478, 491
BsaXI ACNNNNNCTCC 2 cut(s) 473, 503
Bsc4I CCNNNNNNNGG 3 cut(s) 448, 741, 1080
Bse1I ACTGG 1 cut(s) 623
Bse8I GATNNNNATC 2 cut(s) 132, 458
BseBI CCWGG 1 cut(s) 696
BseCI ATCGAT 1 cut(s) 303
BseGI GGATG 2 cut(s) 121, 295
BseJI GATNNNNATC 2 cut(s) 132, 458
BseLI CCNNNNNNNGG 3 cut(s) 448, 741, 1080
BseNI ACTGG 1 cut(s) 623
BseRI GAGGAG 1 cut(s) 941
BseSI GKGCMC 1 cut(s) 1100
BseXI GCAGC 4 cut(s) 533, 1046, 1062, 1096
BsgI GTGCAG 1 cut(s) 382
Bsh1236I CGCG 1 cut(s) 125
BshFI GGCC 2 cut(s) 331, 1002
BshNI GGYRCC 1 cut(s) 72
BshVI ATCGAT 1 cut(s) 303
BsiHKCI CYCGRG 2 cut(s) 617, 1100
BsiSI CCGG 4 cut(s) 479, 492, 509, 1088
BslFI GGGAC 1 cut(s) 965
BslI CCNNNNNNNGG 3 cut(s) 448, 741, 1080
BsmAI GTCTC 2 cut(s) 252, 469
BsmFI GGGAC 1 cut(s) 965
BsnI GGCC 2 cut(s) 331, 1002
Bso31I GGTCTC 2 cut(s) 252, 469
BsoBI CYCGRG 2 cut(s) 617, 1100
Bsp119I TTCGAA 1 cut(s) 972
Bsp1286I GDGCHC 1 cut(s) 1100
Bsp143I GATC 4 cut(s) 88, 189, 613, 988
BspACI CCGC 5 cut(s) 125, 692, 923, 965, 1053
BspANI GGCC 2 cut(s) 331, 1002
BspDI ATCGAT 1 cut(s) 303
BspFNI CGCG 1 cut(s) 125
BspHI TCATGA 1 cut(s) 943
BspLI GGNNCC 2 cut(s) 74, 1003
BspPI GGATC 2 cut(s) 608, 996
BspQI GCTCTTC 1 cut(s) 974
BspT104I TTCGAA 1 cut(s) 972
BspT107I GGYRCC 1 cut(s) 72
BspTNI GGTCTC 2 cut(s) 252, 469
BsrBI CCGCTC 2 cut(s) 923, 967
BsrI ACTGG 1 cut(s) 623
BssMI GATC 4 cut(s) 88, 189, 613, 988
BssNI GRCGYC 1 cut(s) 240
Bst2UI CCWGG 1 cut(s) 696
Bst4CI ACNGT 1 cut(s) 198
Bst6I CTCTTC 4 cut(s) 270, 506, 561, 974
BstACI GRCGYC 1 cut(s) 240
BstBI TTCGAA 1 cut(s) 972
BstC8I GCNNGC 2 cut(s) 534, 1064
BstF5I GGATG 2 cut(s) 121, 295
BstFNI CGCG 1 cut(s) 125
BstH2I RGCGCY 1 cut(s) 611
BstHHI GCGC 1 cut(s) 610
BstKTI GATC 4 cut(s) 91, 192, 616, 991
BstMAI GTCTC 2 cut(s) 252, 469
BstMBI GATC 4 cut(s) 88, 189, 613, 988
BstMWI GCNNNNNNNGC 7 cut(s) 416, 646, 889, 964, 1047, 1056, 1059
BstNI CCWGG 1 cut(s) 696
BstSCI CCNGG 2 cut(s) 507, 694
BstSFI CTRYAG 1 cut(s) 354
BstSLI GKGCMC 1 cut(s) 1100
BstUI CGCG 1 cut(s) 125
BstV1I GCAGC 4 cut(s) 533, 1046, 1062, 1096
BstV2I GAAGAC 2 cut(s) 666, 758
BstX2I RGATCY 1 cut(s) 613
BstYI RGATCY 1 cut(s) 613
Bsu15I ATCGAT 1 cut(s) 303
BsuRI GGCC 2 cut(s) 331, 1002
BsuTUI ATCGAT 1 cut(s) 303
BtgZI GCGATG 1 cut(s) 1054
BtsCI GGATG 2 cut(s) 121, 295
BtsI GCAGTG 3 cut(s) 543, 644, 885
BtsIMutI CAGTG 4 cut(s) 543, 630, 644, 885
Cac8I GCNNGC 2 cut(s) 534, 1064
CciI TCATGA 1 cut(s) 943
CfoI GCGC 1 cut(s) 610
Cfr13I GGNCC 5 cut(s) 47, 56, 511, 993, 1001
ClaI ATCGAT 1 cut(s) 303
CpoI CGGWCCG 1 cut(s) 47
CseI GACGC 1 cut(s) 671
Csp6I GTAC 2 cut(s) 73, 121
CspCI CAANNNNNGTGG 2 cut(s) 836, 871
CspI CGGWCCG 1 cut(s) 47
CviAII CATG 2 cut(s) 835, 944
CviQI GTAC 2 cut(s) 73, 121
DpnI GATC 4 cut(s) 90, 191, 615, 990
DpnII GATC 4 cut(s) 88, 189, 613, 988
DrdI GACNNNNNNGTC 1 cut(s) 445
DseDI GACNNNNNNGTC 1 cut(s) 445
Eam1104I CTCTTC 4 cut(s) 270, 506, 561, 974
EarI CTCTTC 4 cut(s) 270, 506, 561, 974
EciI GGCGGA 1 cut(s) 681
Eco147I AGGCCT 1 cut(s) 331
Eco31I GGTCTC 2 cut(s) 252, 469
Eco32I GATATC 1 cut(s) 301
Eco47I GGWCC 4 cut(s) 47, 56, 511, 993
Eco57I CTGAAG 4 cut(s) 83, 405, 435, 1027
Eco88I CYCGRG 2 cut(s) 617, 1100
EcoRI GAATTC 1 cut(s) 839
EcoRII CCWGG 1 cut(s) 694
EcoRV GATATC 1 cut(s) 301
EcoT22I ATGCAT 1 cut(s) 112
FaeI CATG 2 cut(s) 838, 947
FalI AAGNNNNNCTT 2 cut(s) 953, 985
FaqI GGGAC 1 cut(s) 965
FatI CATG 2 cut(s) 834, 943
FauI CCCGC 1 cut(s) 1046
FblI GTMKAC 2 cut(s) 309, 433
Fnu4HI GCNGC 5 cut(s) 522, 965, 1051, 1060, 1085
FokI GGATG 2 cut(s) 128, 302
Fsp4HI GCNGC 5 cut(s) 522, 965, 1051, 1060, 1085
FspBI CTAG 2 cut(s) 518, 635
GlaI GCGC 1 cut(s) 609
GluI GCNGC 5 cut(s) 522, 965, 1051, 1060, 1085
HaeII RGCGCY 1 cut(s) 611
HaeIII GGCC 2 cut(s) 331, 1002
HapII CCGG 4 cut(s) 479, 492, 509, 1088
HgaI GACGC 1 cut(s) 671
HhaI GCGC 1 cut(s) 610
Hin1I GRCGYC 1 cut(s) 240
Hin1II CATG 2 cut(s) 838, 947
Hin6I GCGC 1 cut(s) 608
HinP1I GCGC 1 cut(s) 608
HincII GTYRAC 1 cut(s) 310
HindII GTYRAC 1 cut(s) 310
HindIII AAGCTT 3 cut(s) 417, 890, 1011
HinfI GANTC 8 cut(s) 128, 278, 386, 427, 707, 745, 862, 978
HpaII CCGG 4 cut(s) 479, 492, 509, 1088
HphI GGTGA 3 cut(s) 22, 235, 305
Hpy166II GTNNAC 2 cut(s) 310, 434
Hpy188III TCNNGA 3 cut(s) 617, 866, 944
Hpy8I GTNNAC 2 cut(s) 310, 434
Hpy99I CGWCG 2 cut(s) 242, 314
HpyAV CCTTC 4 cut(s) 161, 660, 746, 968
HpyCH4III ACNGT 1 cut(s) 198
HpyCH4IV ACGT 2 cut(s) 240, 324
HpyCH4V TGCA 8 cut(s) 110, 363, 578, 768, 832, 873, 1050, 1062
HpyF10VI GCNNNNNNNGC 7 cut(s) 416, 646, 889, 964, 1047, 1056, 1059
HpySE526I ACGT 2 cut(s) 240, 324
Hsp92I GRCGYC 1 cut(s) 240
Hsp92II CATG 2 cut(s) 838, 947
HspAI GCGC 1 cut(s) 608
KpnI GGTACC 1 cut(s) 76
Kzo9I GATC 4 cut(s) 88, 189, 613, 988
LguI GCTCTTC 1 cut(s) 974
LmnI GCTCC 2 cut(s) 920, 1056
Lsp1109I GCAGC 4 cut(s) 533, 1046, 1062, 1096
LweI GCATC 2 cut(s) 119, 1037
MaeI CTAG 2 cut(s) 518, 635
MaeII ACGT 2 cut(s) 240, 324
MaeIII GTNAC 3 cut(s) 211, 241, 1106
MalI GATC 4 cut(s) 90, 191, 615, 990
MbiI CCGCTC 2 cut(s) 923, 967
MboI GATC 4 cut(s) 88, 189, 613, 988
MflI RGATCY 1 cut(s) 613
MhlI GDGCHC 1 cut(s) 1100
MluCI AATT 4 cut(s) 66, 154, 391, 839
MlyI GAGTC 3 cut(s) 287, 395, 987
MmeI TCCRAC 2 cut(s) 429, 966
MnlI CCTC 9 cut(s) 272, 391, 509, 546, 894, 900, 919, 922, 1072
Mph1103I ATGCAT 1 cut(s) 112
MseI TTAA 2 cut(s) 485, 935
MspA1I CMGCKG 1 cut(s) 1053
MspI CCGG 4 cut(s) 479, 492, 509, 1088
MspR9I CCNGG 2 cut(s) 509, 696
MvaI CCWGG 1 cut(s) 696
MvnI CGCG 1 cut(s) 125
MwoI GCNNNNNNNGC 7 cut(s) 416, 646, 889, 964, 1047, 1056, 1059
NciI CCSGG 1 cut(s) 509
NdeII GATC 4 cut(s) 88, 189, 613, 988
NlaIII CATG 2 cut(s) 838, 947
NlaIV GGNNCC 2 cut(s) 74, 1003
NmuCI GTSAC 2 cut(s) 211, 241
NsiI ATGCAT 1 cut(s) 112
NspV TTCGAA 1 cut(s) 972
PagI TCATGA 1 cut(s) 943
PceI AGGCCT 1 cut(s) 331
PciSI GCTCTTC 1 cut(s) 974
PcsI WCGNNNNNNNCGW 3 cut(s) 170, 243, 309
PfeI GAWTC 5 cut(s) 128, 427, 707, 745, 862
PkrI GCNGC 5 cut(s) 523, 966, 1052, 1061, 1086
PleI GAGTC 3 cut(s) 286, 394, 986
PpsI GAGTC 3 cut(s) 286, 394, 986
Psp1406I AACGTT 1 cut(s) 324
Psp6I CCWGG 1 cut(s) 694
PspGI CCWGG 1 cut(s) 694
PspN4I GGNNCC 2 cut(s) 74, 1003
PspPI GGNCC 5 cut(s) 47, 56, 511, 993, 1001
PsuI RGATCY 1 cut(s) 613
RsaI GTAC 2 cut(s) 74, 122
RsaNI GTAC 2 cut(s) 73, 121
Rsr2I CGGWCCG 1 cut(s) 47
RsrII CGGWCCG 1 cut(s) 47
SalI GTCGAC 1 cut(s) 308
SapI GCTCTTC 1 cut(s) 974
SaqAI TTAA 2 cut(s) 485, 935
SatI GCNGC 5 cut(s) 522, 965, 1051, 1060, 1085
Sau3AI GATC 4 cut(s) 88, 189, 613, 988
Sau96I GGNCC 5 cut(s) 47, 56, 511, 993, 1001
SchI GAGTC 3 cut(s) 287, 395, 987
ScrFI CCNGG 2 cut(s) 509, 696
SduI GDGCHC 1 cut(s) 1100
SfaNI GCATC 2 cut(s) 119, 1037
SfcI CTRYAG 1 cut(s) 354
SfuI TTCGAA 1 cut(s) 972
SinI GGWCC 4 cut(s) 47, 56, 511, 993
SmlI CTYRAG 1 cut(s) 959
SmoI CTYRAG 1 cut(s) 959
Sse9I AATT 4 cut(s) 66, 154, 391, 839
SseBI AGGCCT 1 cut(s) 331
SsiI CCGC 5 cut(s) 125, 692, 923, 965, 1053
SspMI CTAG 2 cut(s) 518, 635
StuI AGGCCT 1 cut(s) 331
StyD4I CCNGG 2 cut(s) 507, 694
TaaI ACNGT 1 cut(s) 198
TaiI ACGT 2 cut(s) 243, 327
TaqI TCGA 9 cut(s) 91, 237, 264, 303, 309, 504, 778, 972, 1027
TaqII GACCGA 3 cut(s) 64, 73, 981
TasI AATT 4 cut(s) 66, 154, 391, 839
TauI GCSGC 1 cut(s) 967
TfiI GAWTC 5 cut(s) 128, 427, 707, 745, 862
Tru1I TTAA 2 cut(s) 485, 935
Tru9I TTAA 2 cut(s) 485, 935
TscAI CASTG 4 cut(s) 543, 630, 651, 885
TseFI GTSAC 2 cut(s) 211, 241
TseI GCWGC 4 cut(s) 521, 1050, 1059, 1084
Tsp45I GTSAC 2 cut(s) 211, 241
TspDTI ATGAA 8 cut(s) 17, 44, 285, 672, 699, 886, 932, 960
TspGWI ACGGA 2 cut(s) 230, 398
TspRI CASTG 4 cut(s) 543, 630, 651, 885
VpaK11BI GGWCC 4 cut(s) 47, 56, 511, 993
XapI RAATTY 2 cut(s) 391, 839
XmiI GTMKAC 2 cut(s) 309, 433
XspI CTAG 2 cut(s) 518, 635
ZraI GACGTC 1 cut(s) 241
Zsp2I ATGCAT 1 cut(s) 112
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.