Rh1BG093500

Glutaredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0016741
Physical Location & Seq
Forward (+)
16326613 .. 16328288
1676 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG093500.1

Sequence Viewer

Length: 1098 bp
ATGAAGGGAATGAAAGGAAAACTAGTGAAGAGGTTGAAATCGATCCCAAGAGTTCTTCAGGTGAAGCCACAGGATCGAGTTCTTCAGGTAAGCGCATCAGATGGGTATGTGGAAAACCTTCCAAACATCTCAGTTGTCAAATTCCACAAGGAAGAAACAGAGCAGGACAAGCTCAACTGCAGCAGTGTGAGAGAGCAGCAACAACCTGATATTATTGACGTCGATGAGCTTATGAGAGACCTCCTCACAGATTCTGACGATGACGTCGTCGACGACGACGACAAAGAGAACATGACGCCAAGAATGAAGGCAAAGGACCCAGTTGCTTCTAAGGAGTCTAAATTGATGCAGAGTAAAGCTACTTTCCCAGATTCAGAAGCACCACCGGTACTAGAGTCGGAAAGCCGTAAACAAACACCTCTATCGGAGATTGACATCTCGTCTTTCCGGCGACCGGACTTAGATTCCGGTACCCTCTTCGACCCGGACCTACTCGCCGCCTTTCGGCAAGCCGTTATTGAGTACATGAAATTGAGTGAAGCAGAGAGGAAGTCAATAACAGAGATACAAAATATTGAAAACAATGAAACAGAGGCAGAGGCAGAGGCGGAGCCGGACCCTCTATTAGGCTTCGAAGAGAAATGTCCACCTGGGAGCTTGGAGTCGGTAATTCTCTACACGACAACCCTTCGAGGGATACGAAAGACCTTCGACGATTGCAACAGCATCCGATTTCTGTTAGAGAGCTTTCGGGTTGTGTATTTCGAGCGTGATGTGTCGATGCACATGGAATTTAGACAAGAGCTGTGGAAGATTTTGGATTGCAAATCGGTTCCTCCTAAGCTTTTCATCAAGGGGAGATACATTGGGGGAGCAGAGGAGGTTTTGACGCTTCACGAGCAAGGAAAGCTGCGGCCGCTCCTTGCAGGAGTGCCCTTGGATCAATCAGATGGACCGTGTCAAGGTTGCGCTGGAGTTCGGTTTGTGGTTTGTTTCAAATGCCACGGAAGTCACAAGATCATTGCCCAAGAGGGAGAGTCTAATGTGTGCCCGCAGTGTAACGAAAATGGGTTGATTATATGCCCATATTGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

365

Amino Acids

41.16

Weight (kDa)

4.92

Isoelectric Point (pI)

49.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 223 - 289 1.3e-10 Glutaredoxin
GRXCR1-2_C PF23733 320 - 365 1.5e-16 GRXCR1/2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016741)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g27780 FvH4_1g27790 FvH4_7g05400
malus_domestica MD02G1258300.v1.1 MD07G1062500.v1.1
prunus_persica Prupe.2G073600_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0332071
rosa_laevigata RLG00000029694
rosa_roxburghii Rroxscaffold_4G00318400
rosa_rugosa Rorug01G0098200
rosa_samantha Rh1AG122100 Rh1BG093500 Rh1CG116700
rosa_wichuraiana Rw1G009950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 263
AatII GACGTC 2 cut(s) 222, 267
Acc65I GGTACC 1 cut(s) 470
AccB1I GGYRCC 1 cut(s) 470
AccBSI CCGCTC 1 cut(s) 919
AccI GTMKAC 1 cut(s) 270
AciI CCGC 5 cut(s) 498, 608, 913, 917, 1052
AclWI GGATC 3 cut(s) 37, 81, 948
AcoI YGGCCR 1 cut(s) 914
AcsI RAATTY 2 cut(s) 140, 791
AcuI CTGAAG 2 cut(s) 41, 68
AcyI GRCGYC 3 cut(s) 219, 264, 296
AfaI GTAC 3 cut(s) 390, 472, 524
AfiI CCNNNNNNNGG 5 cut(s) 454, 504, 626, 693, 962
AgeI ACCGGT 1 cut(s) 385
AgsI TTSAA 3 cut(s) 37, 578, 997
AhdI GACNNNNNGTC 1 cut(s) 439
AhlI ACTAGT 1 cut(s) 22
AjnI CCWGG 1 cut(s) 649
AluBI AGCT 8 cut(s) 172, 229, 359, 657, 747, 805, 844, 910
AluI AGCT 8 cut(s) 172, 229, 359, 657, 747, 805, 844, 910
Alw26I GTCTC 1 cut(s) 231
AlwI GGATC 3 cut(s) 37, 81, 948
AlwNI CAGNNNCTG 1 cut(s) 254
AoxI GGCC 1 cut(s) 914
ApeKI GCWGC 3 cut(s) 180, 196, 910
ApoI RAATTY 2 cut(s) 140, 791
AsiGI ACCGGT 1 cut(s) 385
Asp700I GAANNNNTTC 1 cut(s) 117
Asp718I GGTACC 1 cut(s) 470
AspLEI GCGC 2 cut(s) 95, 971
AspS9I GGNCC 4 cut(s) 316, 487, 616, 953
AsuC2I CCSGG 1 cut(s) 485
AsuHPI GGTGA 1 cut(s) 73
AsuII TTCGAA 1 cut(s) 633
AvaII GGWCC 4 cut(s) 316, 487, 616, 953
BaeGI GKGCMC 2 cut(s) 936, 1052
BaeI ACNNNNGTAYC 2 cut(s) 380, 413
BanI GGYRCC 1 cut(s) 470
BauI CACGAG 1 cut(s) 896
BbvI GCAGC 3 cut(s) 192, 208, 897
BccI CCATC 2 cut(s) 95, 944
BceAI ACGGC 2 cut(s) 390, 497
BciT130I CCWGG 1 cut(s) 651
BciVI GTATCC 1 cut(s) 690
BcnI CCSGG 1 cut(s) 485
BcoDI GTCTC 1 cut(s) 231
BcuI ACTAGT 1 cut(s) 22
BfaI CTAG 2 cut(s) 23, 392
BfmI CTRYAG 1 cut(s) 178
BfuI GTATCC 1 cut(s) 690
BisI GCNGC 6 cut(s) 181, 197, 498, 911, 914, 917
BlsI GCNGC 6 cut(s) 182, 198, 499, 912, 915, 918
Bme1390I CCNGG 2 cut(s) 485, 651
Bme18I GGWCC 4 cut(s) 316, 487, 616, 953
BmeRI GACNNNNNGTC 1 cut(s) 439
BmgT120I GGNCC 4 cut(s) 316, 487, 616, 953
BmiI GGNNCC 5 cut(s) 318, 472, 612, 618, 834
BmrFI CCNGG 2 cut(s) 485, 651
BmrI ACTGGG 1 cut(s) 314
BmsI GCATC 4 cut(s) 104, 336, 735, 771
BmuI ACTGGG 1 cut(s) 314
BplI GAGNNNNNCTC 2 cut(s) 228, 260
BpmI CTGGAG 1 cut(s) 993
Bpu10I CCTNAGC 1 cut(s) 840
Bpu14I TTCGAA 1 cut(s) 633
BpuMI CCSGG 1 cut(s) 485
Bsa29I ATCGAT 1 cut(s) 41
BsaBI GATNNNNATC 1 cut(s) 434
BsaHI GRCGYC 3 cut(s) 219, 264, 296
BsaI GGTCTC 1 cut(s) 231
BsaJI CCNNGG 3 cut(s) 650, 936, 1003
BsaWI WCCGGW 3 cut(s) 385, 454, 467
Bsc4I CCNNNNNNNGG 5 cut(s) 454, 504, 626, 693, 962
Bse118I RCCGGY 1 cut(s) 385
Bse1I ACTGG 1 cut(s) 320
Bse3DI GCAATG 1 cut(s) 1020
Bse8I GATNNNNATC 1 cut(s) 434
BseBI CCWGG 1 cut(s) 651
BseCI ATCGAT 1 cut(s) 41
BseDI CCNNGG 3 cut(s) 650, 936, 1003
BseGI GGATG 1 cut(s) 726
BseJI GATNNNNATC 1 cut(s) 434
BseLI CCNNNNNNNGG 5 cut(s) 454, 504, 626, 693, 962
BseMI GCAATG 1 cut(s) 1020
BseMII CTCAG 1 cut(s) 144
BseNI ACTGG 1 cut(s) 320
BseRI GAGGAG 2 cut(s) 233, 893
BseSI GKGCMC 2 cut(s) 936, 1052
BseX3I CGGCCG 1 cut(s) 914
BseXI GCAGC 3 cut(s) 192, 208, 897
Bsh1285I CGRYCG 2 cut(s) 455, 917
BshFI GGCC 1 cut(s) 916
BshNI GGYRCC 1 cut(s) 470
BshTI ACCGGT 1 cut(s) 385
BshVI ATCGAT 1 cut(s) 41
BsiEI CGRYCG 2 cut(s) 455, 917
BsiSI CCGG 6 cut(s) 386, 448, 455, 468, 485, 614
BslI CCNNNNNNNGG 5 cut(s) 454, 504, 626, 693, 962
BsmAI GTCTC 1 cut(s) 231
BsnI GGCC 1 cut(s) 916
Bso31I GGTCTC 1 cut(s) 231
Bsp119I TTCGAA 1 cut(s) 633
Bsp1286I GDGCHC 2 cut(s) 936, 1052
Bsp143I GATC 4 cut(s) 42, 73, 940, 1017
BspACI CCGC 5 cut(s) 498, 608, 913, 917, 1052
BspANI GGCC 1 cut(s) 916
BspCNI CTCAG 1 cut(s) 143
BspDI ATCGAT 1 cut(s) 41
BspLI GGNNCC 5 cut(s) 318, 472, 612, 618, 834
BspMAI CTGCAG 1 cut(s) 182
BspPI GGATC 3 cut(s) 37, 81, 948
BspT104I TTCGAA 1 cut(s) 633
BspT107I GGYRCC 1 cut(s) 470
BspTNI GGTCTC 1 cut(s) 231
BsrBI CCGCTC 1 cut(s) 919
BsrDI GCAATG 1 cut(s) 1020
BsrFI RCCGGY 1 cut(s) 385
BsrI ACTGG 1 cut(s) 320
BssAI RCCGGY 1 cut(s) 385
BssECI CCNNGG 3 cut(s) 650, 936, 1003
BssMI GATC 4 cut(s) 42, 73, 940, 1017
BssNI GRCGYC 3 cut(s) 219, 264, 296
BssSI CACGAG 1 cut(s) 896
BssT1I CCWWGG 1 cut(s) 936
Bst2BI CACGAG 1 cut(s) 896
Bst2UI CCWGG 1 cut(s) 651
Bst4CI ACNGT 1 cut(s) 957
Bst6I CTCTTC 3 cut(s) 23, 482, 630
BstACI GRCGYC 3 cut(s) 219, 264, 296
BstBI TTCGAA 1 cut(s) 633
BstC8I GCNNGC 2 cut(s) 510, 1052
BstDEI CTNAG 4 cut(s) 130, 330, 460, 840
BstDSI CCRYGG 1 cut(s) 1003
BstENI CCTNNNNNAGG 1 cut(s) 624
BstF5I GGATG 1 cut(s) 726
BstHHI GCGC 2 cut(s) 95, 971
BstKTI GATC 4 cut(s) 45, 76, 943, 1020
BstMAI GTCTC 1 cut(s) 231
BstMBI GATC 4 cut(s) 42, 73, 940, 1017
BstMCI CGRYCG 2 cut(s) 455, 917
BstMWI GCNNNNNNNGC 4 cut(s) 169, 898, 907, 916
BstNI CCWGG 1 cut(s) 651
BstSCI CCNGG 2 cut(s) 483, 649
BstSFI CTRYAG 1 cut(s) 178
BstSLI GKGCMC 2 cut(s) 936, 1052
BstV1I GCAGC 3 cut(s) 192, 208, 897
BstZI CGGCCG 1 cut(s) 914
Bsu15I ATCGAT 1 cut(s) 41
BsuI GTATCC 1 cut(s) 690
BsuRI GGCC 1 cut(s) 916
BsuTUI ATCGAT 1 cut(s) 41
BtgI CCRYGG 1 cut(s) 1003
BtsCI GGATG 1 cut(s) 726
BtsI GCAGTG 2 cut(s) 190, 1061
BtsIMutI CAGTG 2 cut(s) 190, 1061
Cac8I GCNNGC 2 cut(s) 510, 1052
CaiI CAGNNNCTG 1 cut(s) 254
CciNI GCGGCCGC 1 cut(s) 914
CfoI GCGC 2 cut(s) 95, 971
Cfr10I RCCGGY 1 cut(s) 385
Cfr13I GGNCC 4 cut(s) 316, 487, 616, 953
ClaI ATCGAT 1 cut(s) 41
CseI GACGC 2 cut(s) 304, 898
Csp6I GTAC 3 cut(s) 389, 471, 523
CspAI ACCGGT 1 cut(s) 385
CspCI CAANNNNNGTGG 2 cut(s) 788, 823
CviAII CATG 3 cut(s) 292, 526, 787
CviQI GTAC 3 cut(s) 389, 471, 523
DdeI CTNAG 4 cut(s) 130, 330, 460, 840
DpnI GATC 4 cut(s) 44, 75, 942, 1019
DpnII GATC 4 cut(s) 42, 73, 940, 1017
DrdI GACNNNNNNGTC 1 cut(s) 263
DriI GACNNNNNGTC 1 cut(s) 439
DseDI GACNNNNNNGTC 1 cut(s) 263
EaeI YGGCCR 1 cut(s) 914
EagI CGGCCG 1 cut(s) 914
Eam1104I CTCTTC 3 cut(s) 23, 482, 630
Eam1105I GACNNNNNGTC 1 cut(s) 439
EarI CTCTTC 3 cut(s) 23, 482, 630
EciI GGCGGA 1 cut(s) 623
EclXI CGGCCG 1 cut(s) 914
Eco130I CCWWGG 1 cut(s) 936
Eco31I GGTCTC 1 cut(s) 231
Eco47I GGWCC 4 cut(s) 316, 487, 616, 953
Eco52I CGGCCG 1 cut(s) 914
Eco57I CTGAAG 2 cut(s) 41, 68
EcoNI CCTNNNNNAGG 1 cut(s) 624
EcoO109I RGGNCCY 1 cut(s) 316
EcoRII CCWGG 1 cut(s) 649
EcoT14I CCWWGG 1 cut(s) 936
ErhI CCWWGG 1 cut(s) 936
FaeI CATG 3 cut(s) 295, 529, 790
FaiI YATR 8 cut(s) 108, 233, 293, 527, 788, 1079, 1081, 1087
FatI CATG 3 cut(s) 291, 525, 786
FauI CCCGC 1 cut(s) 1059
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 6 cut(s) 181, 197, 498, 911, 914, 917
FokI GGATG 1 cut(s) 713
Fsp4HI GCNGC 6 cut(s) 181, 197, 498, 911, 914, 917
FspBI CTAG 2 cut(s) 23, 392
GlaI GCGC 2 cut(s) 94, 970
GluI GCNGC 6 cut(s) 181, 197, 498, 911, 914, 917
GsuI CTGGAG 1 cut(s) 993
HaeIII GGCC 1 cut(s) 916
HapII CCGG 6 cut(s) 386, 448, 455, 468, 485, 614
HgaI GACGC 2 cut(s) 304, 898
HhaI GCGC 2 cut(s) 95, 971
Hin1I GRCGYC 3 cut(s) 219, 264, 296
Hin1II CATG 3 cut(s) 295, 529, 790
Hin6I GCGC 2 cut(s) 93, 969
HinP1I GCGC 2 cut(s) 93, 969
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HindIII AAGCTT 1 cut(s) 842
HinfI GANTC 7 cut(s) 251, 335, 371, 395, 464, 662, 1037
HpaII CCGG 6 cut(s) 386, 448, 455, 468, 485, 614
HphI GGTGA 1 cut(s) 73
Hpy166II GTNNAC 3 cut(s) 271, 410, 647
Hpy188I TCNGA 7 cut(s) 100, 256, 376, 400, 427, 731, 949
Hpy188III TCNNGA 1 cut(s) 896
Hpy8I GTNNAC 3 cut(s) 271, 410, 647
Hpy99I CGWCG 7 cut(s) 224, 269, 272, 275, 278, 281, 716
HpyAV CCTTC 4 cut(s) 128, 301, 698, 718
HpyCH4III ACNGT 1 cut(s) 957
HpyCH4IV ACGT 2 cut(s) 219, 264
HpyCH4V TGCA 6 cut(s) 180, 349, 720, 784, 825, 926
HpyF10VI GCNNNNNNNGC 4 cut(s) 169, 898, 907, 916
HpyF3I CTNAG 4 cut(s) 130, 330, 460, 840
HpySE526I ACGT 2 cut(s) 219, 264
Hsp92I GRCGYC 3 cut(s) 219, 264, 296
Hsp92II CATG 3 cut(s) 295, 529, 790
HspAI GCGC 2 cut(s) 93, 969
KpnI GGTACC 1 cut(s) 474
Kzo9I GATC 4 cut(s) 42, 73, 940, 1017
LmnI GCTCC 4 cut(s) 610, 654, 872, 924
Lsp1109I GCAGC 3 cut(s) 192, 208, 897
LweI GCATC 4 cut(s) 104, 336, 735, 771
MaeI CTAG 2 cut(s) 23, 392
MaeII ACGT 2 cut(s) 219, 264
MaeIII GTNAC 2 cut(s) 1010, 1058
MalI GATC 4 cut(s) 44, 75, 942, 1019
MbiI CCGCTC 1 cut(s) 919
MboI GATC 4 cut(s) 42, 73, 940, 1017
MboII GAAGA 7 cut(s) 40, 47, 74, 164, 469, 647, 823
MhlI GDGCHC 2 cut(s) 936, 1052
MluCI AATT 5 cut(s) 140, 341, 530, 669, 791
MlyI GAGTC 4 cut(s) 344, 404, 671, 1046
MmeI TCCRAC 1 cut(s) 378
MroXI GAANNNNTTC 1 cut(s) 117
MspI CCGG 6 cut(s) 386, 448, 455, 468, 485, 614
MspR9I CCNGG 2 cut(s) 485, 651
MvaI CCWGG 1 cut(s) 651
MwoI GCNNNNNNNGC 4 cut(s) 169, 898, 907, 916
NciI CCSGG 1 cut(s) 485
NdeII GATC 4 cut(s) 42, 73, 940, 1017
NlaIII CATG 3 cut(s) 295, 529, 790
NlaIV GGNNCC 5 cut(s) 318, 472, 612, 618, 834
NmuCI GTSAC 1 cut(s) 1010
NotI GCGGCCGC 1 cut(s) 914
NspV TTCGAA 1 cut(s) 633
PcsI WCGNNNNNNNCGW 5 cut(s) 264, 270, 273, 276, 697
PdmI GAANNNNTTC 1 cut(s) 117
PfeI GAWTC 3 cut(s) 251, 371, 464
PflFI GACNNNGTC 2 cut(s) 266, 957
PinAI ACCGGT 1 cut(s) 385
PkrI GCNGC 6 cut(s) 182, 198, 499, 912, 915, 918
PleI GAGTC 4 cut(s) 343, 403, 670, 1045
PpsI GAGTC 4 cut(s) 343, 403, 670, 1045
PpuMI RGGWCCY 1 cut(s) 316
Psp5II RGGWCCY 1 cut(s) 316
Psp6I CCWGG 1 cut(s) 649
PspGI CCWGG 1 cut(s) 649
PspN4I GGNNCC 5 cut(s) 318, 472, 612, 618, 834
PspPI GGNCC 4 cut(s) 316, 487, 616, 953
PspPPI RGGWCCY 1 cut(s) 316
PstI CTGCAG 1 cut(s) 182
PstNI CAGNNNCTG 1 cut(s) 254
PsyI GACNNNGTC 2 cut(s) 266, 957
RsaI GTAC 3 cut(s) 390, 472, 524
RsaNI GTAC 3 cut(s) 389, 471, 523
SalI GTCGAC 1 cut(s) 269
SatI GCNGC 6 cut(s) 181, 197, 498, 911, 914, 917
Sau3AI GATC 4 cut(s) 42, 73, 940, 1017
Sau96I GGNCC 4 cut(s) 316, 487, 616, 953
SchI GAGTC 4 cut(s) 344, 404, 671, 1046
ScrFI CCNGG 2 cut(s) 485, 651
SduI GDGCHC 2 cut(s) 936, 1052
SfaNI GCATC 4 cut(s) 104, 336, 735, 771
SfcI CTRYAG 1 cut(s) 178
SfuI TTCGAA 1 cut(s) 633
SgrDI CGTCGACG 1 cut(s) 269
SinI GGWCC 4 cut(s) 316, 487, 616, 953
SpeI ACTAGT 1 cut(s) 22
Sse9I AATT 5 cut(s) 140, 341, 530, 669, 791
SsiI CCGC 5 cut(s) 498, 608, 913, 917, 1052
SspI AATATT 1 cut(s) 574
SspMI CTAG 2 cut(s) 23, 392
StyD4I CCNGG 2 cut(s) 483, 649
StyI CCWWGG 1 cut(s) 936
TaaI ACNGT 1 cut(s) 957
TaiI ACGT 2 cut(s) 222, 267
TasI AATT 5 cut(s) 140, 341, 530, 669, 791
TatI WGTACW 1 cut(s) 522
TauI GCSGC 3 cut(s) 500, 916, 919
TfiI GAWTC 3 cut(s) 251, 371, 464
TscAI CASTG 2 cut(s) 190, 1061
TseFI GTSAC 1 cut(s) 1010
TseI GCWGC 3 cut(s) 180, 196, 910
Tsp45I GTSAC 1 cut(s) 1010
TspDTI ATGAA 6 cut(s) 17, 26, 320, 542, 600, 838
TspGWI ACGGA 1 cut(s) 1020
TspRI CASTG 2 cut(s) 190, 1061
Tth111I GACNNNGTC 2 cut(s) 266, 957
VpaK11BI GGWCC 4 cut(s) 316, 487, 616, 953
XagI CCTNNNNNAGG 1 cut(s) 624
XapI RAATTY 2 cut(s) 140, 791
XmiI GTMKAC 1 cut(s) 270
XmnI GAANNNNTTC 1 cut(s) 117
XspI CTAG 2 cut(s) 23, 392
ZraI GACGTC 2 cut(s) 220, 265
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.