Rw1G009950

Glutaredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr1
OG0016741
Physical Location & Seq
Forward (+)
22097940 .. 22099414
1475 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw1G009950.1

Sequence Viewer

Length: 1107 bp
ATGAAGGGAATGAAAGGAAAACTTGTGAAGAGGTTGAAATCAATCCCAAAAGTTCTTCAGGTGAAGCCACAAGATCGAGTTCTTCAGGTAAGCGCATCAGATGGGTATGTGGAAAACCTTCCAAACATCTCAGTTGTCAAATTTCACAAGGAAGAAACAGAGCAGGACAAGCTCAACTACTGCAGCAGTGTGAGAGAGCAGCAACAACCTGATATTATTGACGTCGATGAGCTCATGAGAGACCTCCTCACAGATTCTGAGGATGAAGTCGACGACGACGACGACAAAGAAAACATTAAGCCAAGAGTGAAGGCAAAGGACCCAGTTGCTTCTAAGGAGTTTAAATTGAAGCAGAGTAAAGCTACTTTCCCAGATTCAGAAGCACCACAGGTACTAGAGTCGGAAAGCCGTAAACAAACACCTCTATCGGAGATTGACATCTCGTCTTTCCGGCGACCGGACTTAGATTCCGGTACCCTCTTCGACCCGGACCTACTTGCCGCCTTTCGGCAAGCCGTTATTGAGTACATGAAATTGAGTGAAGCAGAGAGGAAATCAATAACAGAGATTCAAAATATTGAAAACAGTGAAACAGAGGCAGAGGCAGAGGCGGAGGCGGAGCCGGACCCTCTATTAGGCTTCGAAGAGAAATGTCCACCTGGGAGCTGGGAGTCGGTAATTCTCTACACGACAACCCTTCGAGGGATACGAAAGACCTTCGACGATTGCAACAGCATCCGATTTCTGTTAGAGAGCTTTCGGGTTGTGTATTTCGAGCGTGATGTATCGATGCACATGGAATTTAGACAAGAGCTGTGGAAGATTTTGGATTGCAAATCGGTTCCTCCTAAGCTTTTCATCAAGGGGAGATACATTGGGGGAGCAGAGGAGGTTTTGACGCTTCACGAGCAAGGAAAGCTGCGGCCTCTCCTTGCAGGAGTGCCGTTGGATCAATCAGATGGACCGTGTCAAGGTTGCGCTGGAGTTCGGTTTGTGGTTTGTTTCAAATGCCACGGAAGTCACAAGATCATTGCCCAAGAGGGAGAGTCTAATGTGTGCCCGCAGTGTAACGAAAATGGGTTGATTATATGCCCATATTGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

368

Amino Acids

41.65

Weight (kDa)

4.94

Isoelectric Point (pI)

50.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 226 - 292 1.3e-10 Glutaredoxin
GRXCR1-2_C PF23733 323 - 368 1.5e-16 GRXCR1/2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016741)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g27780 FvH4_1g27790 FvH4_7g05400
malus_domestica MD02G1258300.v1.1 MD07G1062500.v1.1
prunus_persica Prupe.2G073600_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0332071
rosa_laevigata RLG00000029694
rosa_roxburghii Rroxscaffold_4G00318400
rosa_rugosa Rorug01G0098200
rosa_samantha Rh1AG122100 Rh1BG093500 Rh1CG116700
rosa_wichuraiana Rw1G009950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 225
Acc65I GGTACC 1 cut(s) 473
AccB1I GGYRCC 1 cut(s) 473
AccI GTMKAC 1 cut(s) 270
AciI CCGC 5 cut(s) 501, 611, 617, 922, 1061
AclWI GGATC 1 cut(s) 957
AcsI RAATTY 2 cut(s) 140, 800
AcuI CTGAAG 2 cut(s) 41, 68
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 3 cut(s) 393, 475, 527
AfiI CCNNNNNNNGG 5 cut(s) 457, 507, 635, 702, 971
AgsI TTSAA 5 cut(s) 37, 349, 572, 581, 1006
AhdI GACNNNNNGTC 1 cut(s) 442
AjnI CCWGG 1 cut(s) 658
AluBI AGCT 8 cut(s) 172, 232, 362, 666, 756, 814, 853, 919
AluI AGCT 8 cut(s) 172, 232, 362, 666, 756, 814, 853, 919
Alw21I GWGCWC 1 cut(s) 234
Alw26I GTCTC 1 cut(s) 234
AlwI GGATC 1 cut(s) 957
AlwNI CAGNNNCTG 1 cut(s) 257
AoxI GGCC 1 cut(s) 923
ApeKI GCWGC 3 cut(s) 183, 199, 919
ApoI RAATTY 2 cut(s) 140, 800
Asp700I GAANNNNTTC 1 cut(s) 117
Asp718I GGTACC 1 cut(s) 473
AspLEI GCGC 2 cut(s) 95, 980
AspS9I GGNCC 4 cut(s) 319, 490, 625, 962
AsuC2I CCSGG 1 cut(s) 488
AsuHPI GGTGA 1 cut(s) 73
AsuII TTCGAA 1 cut(s) 642
AvaII GGWCC 4 cut(s) 319, 490, 625, 962
BaeGI GKGCMC 1 cut(s) 1061
BaeI ACNNNNGTAYC 2 cut(s) 383, 416
BanI GGYRCC 1 cut(s) 473
BanII GRGCYC 1 cut(s) 234
BauI CACGAG 1 cut(s) 905
Bbv12I GWGCWC 1 cut(s) 234
BbvI GCAGC 3 cut(s) 195, 211, 906
BccI CCATC 2 cut(s) 95, 953
BceAI ACGGC 3 cut(s) 393, 500, 928
BciT130I CCWGG 1 cut(s) 660
BciVI GTATCC 1 cut(s) 699
BcnI CCSGG 1 cut(s) 488
BcoDI GTCTC 1 cut(s) 234
BfaI CTAG 1 cut(s) 395
BfmI CTRYAG 1 cut(s) 181
BfuI GTATCC 1 cut(s) 699
BisI GCNGC 5 cut(s) 184, 200, 501, 920, 923
BlsI GCNGC 5 cut(s) 185, 201, 502, 921, 924
Bme1390I CCNGG 2 cut(s) 488, 660
Bme18I GGWCC 4 cut(s) 319, 490, 625, 962
BmeRI GACNNNNNGTC 1 cut(s) 442
BmgT120I GGNCC 4 cut(s) 319, 490, 625, 962
BmiI GGNNCC 5 cut(s) 321, 475, 621, 627, 843
BmrFI CCNGG 2 cut(s) 488, 660
BmrI ACTGGG 1 cut(s) 317
BmsI GCATC 3 cut(s) 104, 744, 780
BmuI ACTGGG 1 cut(s) 317
BplI GAGNNNNNCTC 2 cut(s) 231, 263
BpmI CTGGAG 1 cut(s) 1002
Bpu10I CCTNAGC 1 cut(s) 849
Bpu14I TTCGAA 1 cut(s) 642
BpuMI CCSGG 1 cut(s) 488
Bsa29I ATCGAT 1 cut(s) 788
BsaBI GATNNNNATC 1 cut(s) 437
BsaHI GRCGYC 1 cut(s) 222
BsaI GGTCTC 1 cut(s) 234
BsaJI CCNNGG 2 cut(s) 659, 1012
BsaWI WCCGGW 2 cut(s) 457, 470
Bsc4I CCNNNNNNNGG 5 cut(s) 457, 507, 635, 702, 971
Bse1I ACTGG 1 cut(s) 323
Bse3DI GCAATG 1 cut(s) 1029
Bse8I GATNNNNATC 1 cut(s) 437
BseBI CCWGG 1 cut(s) 660
BseCI ATCGAT 1 cut(s) 788
BseDI CCNNGG 2 cut(s) 659, 1012
BseGI GGATG 2 cut(s) 268, 735
BseJI GATNNNNATC 1 cut(s) 437
BseLI CCNNNNNNNGG 5 cut(s) 457, 507, 635, 702, 971
BseMI GCAATG 1 cut(s) 1029
BseMII CTCAG 2 cut(s) 144, 249
BseNI ACTGG 1 cut(s) 323
BseRI GAGGAG 2 cut(s) 236, 902
BseSI GKGCMC 1 cut(s) 1061
BseXI GCAGC 3 cut(s) 195, 211, 906
BseYI CCCAGC 1 cut(s) 666
Bsh1285I CGRYCG 1 cut(s) 458
BshFI GGCC 1 cut(s) 925
BshNI GGYRCC 1 cut(s) 473
BshVI ATCGAT 1 cut(s) 788
BsiEI CGRYCG 1 cut(s) 458
BsiHKAI GWGCWC 1 cut(s) 234
BsiSI CCGG 5 cut(s) 451, 458, 471, 488, 623
BslI CCNNNNNNNGG 5 cut(s) 457, 507, 635, 702, 971
BsmAI GTCTC 1 cut(s) 234
BsnI GGCC 1 cut(s) 925
Bso31I GGTCTC 1 cut(s) 234
Bsp119I TTCGAA 1 cut(s) 642
Bsp1286I GDGCHC 2 cut(s) 234, 1061
Bsp143I GATC 3 cut(s) 73, 949, 1026
BspACI CCGC 5 cut(s) 501, 611, 617, 922, 1061
BspANI GGCC 1 cut(s) 925
BspCNI CTCAG 2 cut(s) 143, 250
BspDI ATCGAT 1 cut(s) 788
BspHI TCATGA 1 cut(s) 234
BspLI GGNNCC 5 cut(s) 321, 475, 621, 627, 843
BspMAI CTGCAG 1 cut(s) 185
BspPI GGATC 1 cut(s) 957
BspT104I TTCGAA 1 cut(s) 642
BspT107I GGYRCC 1 cut(s) 473
BspTNI GGTCTC 1 cut(s) 234
BsrDI GCAATG 1 cut(s) 1029
BsrI ACTGG 1 cut(s) 323
BssECI CCNNGG 2 cut(s) 659, 1012
BssMI GATC 3 cut(s) 73, 949, 1026
BssNI GRCGYC 1 cut(s) 222
BssSI CACGAG 1 cut(s) 905
Bst2BI CACGAG 1 cut(s) 905
Bst2UI CCWGG 1 cut(s) 660
Bst4CI ACNGT 2 cut(s) 587, 966
Bst6I CTCTTC 3 cut(s) 23, 485, 639
BstACI GRCGYC 1 cut(s) 222
BstBI TTCGAA 1 cut(s) 642
BstC8I GCNNGC 2 cut(s) 513, 1061
BstDEI CTNAG 5 cut(s) 130, 258, 333, 463, 849
BstDSI CCRYGG 1 cut(s) 1012
BstENI CCTNNNNNAGG 1 cut(s) 633
BstF5I GGATG 2 cut(s) 268, 735
BstHHI GCGC 2 cut(s) 95, 980
BstKTI GATC 3 cut(s) 76, 952, 1029
BstMAI GTCTC 1 cut(s) 234
BstMBI GATC 3 cut(s) 73, 949, 1026
BstMCI CGRYCG 1 cut(s) 458
BstMWI GCNNNNNNNGC 3 cut(s) 169, 907, 916
BstNI CCWGG 1 cut(s) 660
BstSCI CCNGG 2 cut(s) 486, 658
BstSFI CTRYAG 1 cut(s) 181
BstSLI GKGCMC 1 cut(s) 1061
BstV1I GCAGC 3 cut(s) 195, 211, 906
Bsu15I ATCGAT 1 cut(s) 788
BsuI GTATCC 1 cut(s) 699
BsuRI GGCC 1 cut(s) 925
BsuTUI ATCGAT 1 cut(s) 788
BtgI CCRYGG 1 cut(s) 1012
BtsCI GGATG 2 cut(s) 268, 735
BtsI GCAGTG 2 cut(s) 193, 1070
BtsIMutI CAGTG 3 cut(s) 193, 592, 1070
Cac8I GCNNGC 2 cut(s) 513, 1061
CaiI CAGNNNCTG 1 cut(s) 257
CciI TCATGA 1 cut(s) 234
CfoI GCGC 2 cut(s) 95, 980
Cfr13I GGNCC 4 cut(s) 319, 490, 625, 962
ClaI ATCGAT 1 cut(s) 788
CseI GACGC 1 cut(s) 907
Csp6I GTAC 3 cut(s) 392, 474, 526
CspCI CAANNNNNGTGG 2 cut(s) 797, 832
CviAII CATG 3 cut(s) 235, 529, 796
CviQI GTAC 3 cut(s) 392, 474, 526
DdeI CTNAG 5 cut(s) 130, 258, 333, 463, 849
DpnI GATC 3 cut(s) 75, 951, 1028
DpnII GATC 3 cut(s) 73, 949, 1026
DraI TTTAAA 1 cut(s) 343
DriI GACNNNNNGTC 1 cut(s) 442
Eam1104I CTCTTC 3 cut(s) 23, 485, 639
Eam1105I GACNNNNNGTC 1 cut(s) 442
EarI CTCTTC 3 cut(s) 23, 485, 639
EciI GGCGGA 2 cut(s) 626, 632
Ecl136II GAGCTC 1 cut(s) 232
Eco24I GRGCYC 1 cut(s) 234
Eco31I GGTCTC 1 cut(s) 234
Eco47I GGWCC 4 cut(s) 319, 490, 625, 962
Eco53kI GAGCTC 1 cut(s) 232
Eco57I CTGAAG 2 cut(s) 41, 68
EcoICRI GAGCTC 1 cut(s) 232
EcoNI CCTNNNNNAGG 1 cut(s) 633
EcoO109I RGGNCCY 1 cut(s) 319
EcoRII CCWGG 1 cut(s) 658
EcoT38I GRGCYC 1 cut(s) 234
FaeI CATG 3 cut(s) 238, 532, 799
FaiI YATR 7 cut(s) 108, 236, 530, 797, 1088, 1090, 1096
FalI AAGNNNNNCTT 1 cut(s) 38
FatI CATG 3 cut(s) 234, 528, 795
FauI CCCGC 1 cut(s) 1068
FblI GTMKAC 1 cut(s) 270
Fnu4HI GCNGC 5 cut(s) 184, 200, 501, 920, 923
FokI GGATG 2 cut(s) 275, 722
FriOI GRGCYC 1 cut(s) 234
Fsp4HI GCNGC 5 cut(s) 184, 200, 501, 920, 923
FspBI CTAG 1 cut(s) 395
GlaI GCGC 2 cut(s) 94, 979
GluI GCNGC 5 cut(s) 184, 200, 501, 920, 923
GsaI CCCAGC 1 cut(s) 670
GsuI CTGGAG 1 cut(s) 1002
HaeIII GGCC 1 cut(s) 925
HapII CCGG 5 cut(s) 451, 458, 471, 488, 623
HgaI GACGC 1 cut(s) 907
HhaI GCGC 2 cut(s) 95, 980
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 3 cut(s) 238, 532, 799
Hin6I GCGC 2 cut(s) 93, 978
HinP1I GCGC 2 cut(s) 93, 978
HincII GTYRAC 1 cut(s) 271
HindII GTYRAC 1 cut(s) 271
HindIII AAGCTT 1 cut(s) 851
HinfI GANTC 7 cut(s) 254, 374, 398, 467, 568, 671, 1046
HpaII CCGG 5 cut(s) 451, 458, 471, 488, 623
HphI GGTGA 1 cut(s) 73
Hpy166II GTNNAC 3 cut(s) 271, 413, 656
Hpy188I TCNGA 7 cut(s) 100, 259, 379, 403, 430, 740, 958
Hpy188III TCNNGA 2 cut(s) 235, 905
Hpy8I GTNNAC 3 cut(s) 271, 413, 656
Hpy99I CGWCG 6 cut(s) 227, 275, 278, 281, 284, 725
HpyAV CCTTC 4 cut(s) 128, 304, 707, 727
HpyCH4III ACNGT 2 cut(s) 587, 966
HpyCH4IV ACGT 1 cut(s) 222
HpyCH4V TGCA 5 cut(s) 183, 729, 793, 834, 935
HpyF10VI GCNNNNNNNGC 3 cut(s) 169, 907, 916
HpyF3I CTNAG 5 cut(s) 130, 258, 333, 463, 849
HpySE526I ACGT 1 cut(s) 222
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 3 cut(s) 238, 532, 799
HspAI GCGC 2 cut(s) 93, 978
KpnI GGTACC 1 cut(s) 477
Kzo9I GATC 3 cut(s) 73, 949, 1026
LmnI GCTCC 3 cut(s) 619, 663, 881
Lsp1109I GCAGC 3 cut(s) 195, 211, 906
LweI GCATC 3 cut(s) 104, 744, 780
MaeI CTAG 1 cut(s) 395
MaeII ACGT 1 cut(s) 222
MaeIII GTNAC 2 cut(s) 1019, 1067
MalI GATC 3 cut(s) 75, 951, 1028
MboI GATC 3 cut(s) 73, 949, 1026
MboII GAAGA 7 cut(s) 40, 47, 74, 164, 472, 656, 832
MhlI GDGCHC 2 cut(s) 234, 1061
MluCI AATT 5 cut(s) 140, 344, 533, 678, 800
MlyI GAGTC 3 cut(s) 407, 680, 1055
MmeI TCCRAC 2 cut(s) 381, 927
MroXI GAANNNNTTC 1 cut(s) 117
MseI TTAA 2 cut(s) 297, 342
MspI CCGG 5 cut(s) 451, 458, 471, 488, 623
MspR9I CCNGG 2 cut(s) 488, 660
MvaI CCWGG 1 cut(s) 660
MwoI GCNNNNNNNGC 3 cut(s) 169, 907, 916
NciI CCSGG 1 cut(s) 488
NdeII GATC 3 cut(s) 73, 949, 1026
NlaIII CATG 3 cut(s) 238, 532, 799
NlaIV GGNNCC 5 cut(s) 321, 475, 621, 627, 843
NmuCI GTSAC 1 cut(s) 1019
NspV TTCGAA 1 cut(s) 642
PagI TCATGA 1 cut(s) 234
PcsI WCGNNNNNNNCGW 3 cut(s) 276, 279, 706
PdmI GAANNNNTTC 1 cut(s) 117
PfeI GAWTC 4 cut(s) 254, 374, 467, 568
PflFI GACNNNGTC 1 cut(s) 966
PkrI GCNGC 5 cut(s) 185, 201, 502, 921, 924
PleI GAGTC 3 cut(s) 406, 679, 1054
PpsI GAGTC 3 cut(s) 406, 679, 1054
PpuMI RGGWCCY 1 cut(s) 319
Psp124BI GAGCTC 1 cut(s) 234
Psp5II RGGWCCY 1 cut(s) 319
Psp6I CCWGG 1 cut(s) 658
PspFI CCCAGC 1 cut(s) 666
PspGI CCWGG 1 cut(s) 658
PspN4I GGNNCC 5 cut(s) 321, 475, 621, 627, 843
PspPI GGNCC 4 cut(s) 319, 490, 625, 962
PspPPI RGGWCCY 1 cut(s) 319
PstI CTGCAG 1 cut(s) 185
PstNI CAGNNNCTG 1 cut(s) 257
PsyI GACNNNGTC 1 cut(s) 966
RsaI GTAC 3 cut(s) 393, 475, 527
RsaNI GTAC 3 cut(s) 392, 474, 526
SacI GAGCTC 1 cut(s) 234
SalI GTCGAC 1 cut(s) 269
SaqAI TTAA 2 cut(s) 297, 342
SatI GCNGC 5 cut(s) 184, 200, 501, 920, 923
Sau3AI GATC 3 cut(s) 73, 949, 1026
Sau96I GGNCC 4 cut(s) 319, 490, 625, 962
SchI GAGTC 3 cut(s) 407, 680, 1055
ScrFI CCNGG 2 cut(s) 488, 660
SduI GDGCHC 2 cut(s) 234, 1061
SfaNI GCATC 3 cut(s) 104, 744, 780
SfcI CTRYAG 1 cut(s) 181
SfuI TTCGAA 1 cut(s) 642
SinI GGWCC 4 cut(s) 319, 490, 625, 962
Sse9I AATT 5 cut(s) 140, 344, 533, 678, 800
SsiI CCGC 5 cut(s) 501, 611, 617, 922, 1061
SspI AATATT 1 cut(s) 577
SspMI CTAG 1 cut(s) 395
SstI GAGCTC 1 cut(s) 234
StyD4I CCNGG 2 cut(s) 486, 658
TaaI ACNGT 2 cut(s) 587, 966
TaiI ACGT 1 cut(s) 225
TaqI TCGA 9 cut(s) 76, 225, 270, 483, 642, 700, 720, 774, 788
TasI AATT 5 cut(s) 140, 344, 533, 678, 800
TatI WGTACW 1 cut(s) 525
TauI GCSGC 2 cut(s) 503, 925
TfiI GAWTC 4 cut(s) 254, 374, 467, 568
Tru1I TTAA 2 cut(s) 297, 342
Tru9I TTAA 2 cut(s) 297, 342
TscAI CASTG 3 cut(s) 193, 592, 1070
TseFI GTSAC 1 cut(s) 1019
TseI GCWGC 3 cut(s) 183, 199, 919
Tsp45I GTSAC 1 cut(s) 1019
TspDTI ATGAA 5 cut(s) 17, 26, 279, 545, 847
TspGWI ACGGA 1 cut(s) 1029
TspRI CASTG 3 cut(s) 193, 592, 1070
Tth111I GACNNNGTC 1 cut(s) 966
VpaK11BI GGWCC 4 cut(s) 319, 490, 625, 962
XagI CCTNNNNNAGG 1 cut(s) 633
XapI RAATTY 2 cut(s) 140, 800
XcmI CCANNNNNNNNNTGG 1 cut(s) 663
XmiI GTMKAC 1 cut(s) 270
XmnI GAANNNNTTC 1 cut(s) 117
XspI CTAG 1 cut(s) 395
ZraI GACGTC 1 cut(s) 223
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.