Rroxscaffold_4G00318400

Glutaredoxin

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0016741
Physical Location & Seq
Reverse (-)
44532100 .. 44533191
1092 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00318400.1

Sequence Viewer

Length: 1092 bp
ATGAAGGGAATGAAAGGAAAACTAGTGAAGAGGTTGAAATCTATCCCAAGAGTTCTTCAGGTGAAGCCACAGGATCGAGTTCTTCAGGTAAGCGCATCAGATGGGTATGTGGAAAACCTTCCAAACATCTCAGTTGTCAAATTCCACAAGGACGAAACAGAGCAGGACAAGCTCAGCTGCAGCAGTGTGAGAGAGCAGCAACAACCTGATATTATTGACGTCGATGAGCTCATGAGAGACCTCCTCACAGATTCTGAGGGTGAAGTCGACGACAACAAAGAAAACATTACGCCAAGAGTGAAGGCAAAGGACCCAGTTGCTTCTAAGGAGTTTAAATTGAAGCAGAGTAAAGCTACTTTTCCAGACTTAGAAGCACCACCGGTACTAGAGTCGGAAAGCCGTAAACAAACACCTCTATCGGAGATTGACATCTCGTCTTTCCGGCGACCGGACTTAGATTCCGGTACCCTCTTCGACCCGGACCTACTCGCCGCCTTTCGGCAAGCCGTTATTGAGTACATGAAATTGAGTGAAGCAGAGAGGAAGTCAATAACAGAGATACAAAATATTGAAAACAATGAAACAGAGGCAGAGGCAGAGGCGGAGCCGGACCCTCTATTAGGCTTCGAAGAGAAATGTCCACCTGGGAGCTTGGAGTCGGTAGTTCTCTACACGACAACCCTTCGAGGGATACGAAAGACCTTCGACGATTGCAACAGCATCCGATTTCTGTTAGAGAGCTTTCGGGTTGTATATTTCGAGCGTGATGTGTCGATGCACATGGAATTCAGACAAGAGCTGTGGAAGATTTTGGATTGCAAATCGGTTCCTCCTAAGCTTTTCATCAAGGGCAGATACATTGGGGGAGCAGAGGAGGTTTTGACGCTTCACGAGCAAGGAAAGCTGCGGCCGCTCCTTGCAGGAGTGCCGTTGGATCAATCAGATGGTCCGTGTCAAGGTTGCGCTGGAGTTCGGTTTGTGGTTTGTTTCAAATGCCATGGAAGTCACAAGATCATTGCCCAAGAGGGAGAGTCTAATGTGTGCCCGCAGTGTAACGAAAATGGGTTGATTATATGCCCATATTGTTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Glutaredoxin

Protein Analysis

363

Amino Acids

40.9

Weight (kDa)

5.1

Isoelectric Point (pI)

50.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glutaredoxin PF00462 221 - 287 5.4e-11 Glutaredoxin
GRXCR1-2_C PF23733 318 - 363 1.5e-16 GRXCR1/2, C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016741)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g27780 FvH4_1g27790 FvH4_7g05400
malus_domestica MD02G1258300.v1.1 MD07G1062500.v1.1
prunus_persica Prupe.2G073600_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0332071
rosa_laevigata RLG00000029694
rosa_roxburghii Rroxscaffold_4G00318400
rosa_rugosa Rorug01G0098200
rosa_samantha Rh1AG122100 Rh1BG093500 Rh1CG116700
rosa_wichuraiana Rw1G009950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 222
Acc65I GGTACC 1 cut(s) 464
AccB1I GGYRCC 1 cut(s) 464
AccBSI CCGCTC 1 cut(s) 913
AccI GTMKAC 1 cut(s) 267
AciI CCGC 5 cut(s) 492, 602, 907, 911, 1046
AclWI GGATC 2 cut(s) 81, 942
AcoI YGGCCR 1 cut(s) 908
AcsI RAATTY 2 cut(s) 140, 785
AcuI CTGAAG 2 cut(s) 41, 68
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 3 cut(s) 384, 466, 518
AfiI CCNNNNNNNGG 5 cut(s) 448, 498, 620, 687, 956
AgeI ACCGGT 1 cut(s) 379
AgsI TTSAA 4 cut(s) 37, 340, 572, 991
AhdI GACNNNNNGTC 1 cut(s) 433
AhlI ACTAGT 1 cut(s) 22
AjnI CCWGG 1 cut(s) 643
AluBI AGCT 9 cut(s) 172, 177, 229, 353, 651, 741, 799, 838, 904
AluI AGCT 9 cut(s) 172, 177, 229, 353, 651, 741, 799, 838, 904
Alw21I GWGCWC 1 cut(s) 231
Alw26I GTCTC 1 cut(s) 231
AlwI GGATC 2 cut(s) 81, 942
AlwNI CAGNNNCTG 1 cut(s) 254
AoxI GGCC 1 cut(s) 908
ApeKI GCWGC 4 cut(s) 177, 180, 196, 904
ApoI RAATTY 2 cut(s) 140, 785
AsiGI ACCGGT 1 cut(s) 379
Asp700I GAANNNNTTC 1 cut(s) 117
Asp718I GGTACC 1 cut(s) 464
AspLEI GCGC 2 cut(s) 95, 965
AspS9I GGNCC 4 cut(s) 310, 481, 610, 947
AsuC2I CCSGG 1 cut(s) 479
AsuHPI GGTGA 2 cut(s) 73, 272
AsuII TTCGAA 1 cut(s) 627
AvaII GGWCC 4 cut(s) 310, 481, 610, 947
BaeGI GKGCMC 1 cut(s) 1046
BaeI ACNNNNGTAYC 2 cut(s) 374, 407
BanI GGYRCC 1 cut(s) 464
BanII GRGCYC 1 cut(s) 231
BauI CACGAG 1 cut(s) 890
Bbv12I GWGCWC 1 cut(s) 231
BbvI GCAGC 4 cut(s) 164, 192, 208, 891
BccI CCATC 2 cut(s) 95, 938
BceAI ACGGC 3 cut(s) 384, 491, 913
BciT130I CCWGG 1 cut(s) 645
BciVI GTATCC 1 cut(s) 684
BcnI CCSGG 1 cut(s) 479
BcoDI GTCTC 1 cut(s) 231
BcuI ACTAGT 1 cut(s) 22
BfaI CTAG 2 cut(s) 23, 386
BfmI CTRYAG 1 cut(s) 178
BfuI GTATCC 1 cut(s) 684
BisI GCNGC 7 cut(s) 178, 181, 197, 492, 905, 908, 911
BlpI GCTNAGC 1 cut(s) 173
BlsI GCNGC 7 cut(s) 179, 182, 198, 493, 906, 909, 912
Bme1390I CCNGG 2 cut(s) 479, 645
Bme18I GGWCC 4 cut(s) 310, 481, 610, 947
BmeRI GACNNNNNGTC 1 cut(s) 433
BmgT120I GGNCC 4 cut(s) 310, 481, 610, 947
BmiI GGNNCC 5 cut(s) 312, 466, 606, 612, 828
BmrFI CCNGG 2 cut(s) 479, 645
BmrI ACTGGG 1 cut(s) 308
BmsI GCATC 3 cut(s) 104, 729, 765
BmuI ACTGGG 1 cut(s) 308
BplI GAGNNNNNCTC 2 cut(s) 228, 260
BpmI CTGGAG 1 cut(s) 987
Bpu10I CCTNAGC 1 cut(s) 834
Bpu1102I GCTNAGC 1 cut(s) 173
Bpu14I TTCGAA 1 cut(s) 627
BpuMI CCSGG 1 cut(s) 479
BsaBI GATNNNNATC 1 cut(s) 428
BsaHI GRCGYC 1 cut(s) 219
BsaI GGTCTC 1 cut(s) 231
BsaJI CCNNGG 2 cut(s) 644, 997
BsaWI WCCGGW 3 cut(s) 379, 448, 461
Bsc4I CCNNNNNNNGG 5 cut(s) 448, 498, 620, 687, 956
Bse118I RCCGGY 1 cut(s) 379
Bse1I ACTGG 1 cut(s) 314
Bse3DI GCAATG 1 cut(s) 1014
Bse8I GATNNNNATC 1 cut(s) 428
BseBI CCWGG 1 cut(s) 645
BseDI CCNNGG 2 cut(s) 644, 997
BseGI GGATG 1 cut(s) 720
BseJI GATNNNNATC 1 cut(s) 428
BseLI CCNNNNNNNGG 5 cut(s) 448, 498, 620, 687, 956
BseMI GCAATG 1 cut(s) 1014
BseMII CTCAG 3 cut(s) 144, 187, 246
BseNI ACTGG 1 cut(s) 314
BseRI GAGGAG 2 cut(s) 233, 887
BseSI GKGCMC 1 cut(s) 1046
BseX3I CGGCCG 1 cut(s) 908
BseXI GCAGC 4 cut(s) 164, 192, 208, 891
Bsh1285I CGRYCG 2 cut(s) 449, 911
BshFI GGCC 1 cut(s) 910
BshNI GGYRCC 1 cut(s) 464
BshTI ACCGGT 1 cut(s) 379
BsiEI CGRYCG 2 cut(s) 449, 911
BsiHKAI GWGCWC 1 cut(s) 231
BsiSI CCGG 6 cut(s) 380, 442, 449, 462, 479, 608
BslI CCNNNNNNNGG 5 cut(s) 448, 498, 620, 687, 956
BsmAI GTCTC 1 cut(s) 231
BsnI GGCC 1 cut(s) 910
Bso31I GGTCTC 1 cut(s) 231
Bsp119I TTCGAA 1 cut(s) 627
Bsp1286I GDGCHC 2 cut(s) 231, 1046
Bsp143I GATC 3 cut(s) 73, 934, 1011
Bsp1720I GCTNAGC 1 cut(s) 173
Bsp19I CCATGG 1 cut(s) 997
BspACI CCGC 5 cut(s) 492, 602, 907, 911, 1046
BspANI GGCC 1 cut(s) 910
BspCNI CTCAG 3 cut(s) 143, 186, 247
BspHI TCATGA 1 cut(s) 231
BspLI GGNNCC 5 cut(s) 312, 466, 606, 612, 828
BspMAI CTGCAG 1 cut(s) 182
BspPI GGATC 2 cut(s) 81, 942
BspT104I TTCGAA 1 cut(s) 627
BspT107I GGYRCC 1 cut(s) 464
BspTNI GGTCTC 1 cut(s) 231
BsrBI CCGCTC 1 cut(s) 913
BsrDI GCAATG 1 cut(s) 1014
BsrFI RCCGGY 1 cut(s) 379
BsrI ACTGG 1 cut(s) 314
BssAI RCCGGY 1 cut(s) 379
BssECI CCNNGG 2 cut(s) 644, 997
BssMI GATC 3 cut(s) 73, 934, 1011
BssNI GRCGYC 1 cut(s) 219
BssSI CACGAG 1 cut(s) 890
BssT1I CCWWGG 1 cut(s) 997
Bst2BI CACGAG 1 cut(s) 890
Bst2UI CCWGG 1 cut(s) 645
Bst6I CTCTTC 3 cut(s) 23, 476, 624
BstACI GRCGYC 1 cut(s) 219
BstBI TTCGAA 1 cut(s) 627
BstC8I GCNNGC 2 cut(s) 504, 1046
BstDEI CTNAG 7 cut(s) 130, 173, 255, 324, 367, 454, 834
BstDSI CCRYGG 1 cut(s) 997
BstENI CCTNNNNNAGG 1 cut(s) 618
BstF5I GGATG 1 cut(s) 720
BstHHI GCGC 2 cut(s) 95, 965
BstKTI GATC 3 cut(s) 76, 937, 1014
BstMAI GTCTC 1 cut(s) 231
BstMBI GATC 3 cut(s) 73, 934, 1011
BstMCI CGRYCG 2 cut(s) 449, 911
BstMWI GCNNNNNNNGC 4 cut(s) 169, 892, 901, 910
BstNI CCWGG 1 cut(s) 645
BstSCI CCNGG 2 cut(s) 477, 643
BstSFI CTRYAG 1 cut(s) 178
BstSLI GKGCMC 1 cut(s) 1046
BstV1I GCAGC 4 cut(s) 164, 192, 208, 891
BstZI CGGCCG 1 cut(s) 908
BsuI GTATCC 1 cut(s) 684
BsuRI GGCC 1 cut(s) 910
BtgI CCRYGG 1 cut(s) 997
BtsCI GGATG 1 cut(s) 720
BtsI GCAGTG 2 cut(s) 190, 1055
BtsIMutI CAGTG 2 cut(s) 190, 1055
Cac8I GCNNGC 2 cut(s) 504, 1046
CaiI CAGNNNCTG 1 cut(s) 254
CciI TCATGA 1 cut(s) 231
CciNI GCGGCCGC 1 cut(s) 908
CfoI GCGC 2 cut(s) 95, 965
Cfr10I RCCGGY 1 cut(s) 379
Cfr13I GGNCC 4 cut(s) 310, 481, 610, 947
CseI GACGC 1 cut(s) 892
Csp6I GTAC 3 cut(s) 383, 465, 517
CspAI ACCGGT 1 cut(s) 379
CspCI CAANNNNNGTGG 2 cut(s) 782, 817
CviAII CATG 4 cut(s) 232, 520, 781, 998
CviQI GTAC 3 cut(s) 383, 465, 517
DdeI CTNAG 7 cut(s) 130, 173, 255, 324, 367, 454, 834
DpnI GATC 3 cut(s) 75, 936, 1013
DpnII GATC 3 cut(s) 73, 934, 1011
DraI TTTAAA 1 cut(s) 334
DriI GACNNNNNGTC 1 cut(s) 433
EaeI YGGCCR 1 cut(s) 908
EagI CGGCCG 1 cut(s) 908
Eam1104I CTCTTC 3 cut(s) 23, 476, 624
Eam1105I GACNNNNNGTC 1 cut(s) 433
EarI CTCTTC 3 cut(s) 23, 476, 624
EciI GGCGGA 1 cut(s) 617
Ecl136II GAGCTC 1 cut(s) 229
EclXI CGGCCG 1 cut(s) 908
Eco130I CCWWGG 1 cut(s) 997
Eco24I GRGCYC 1 cut(s) 231
Eco31I GGTCTC 1 cut(s) 231
Eco47I GGWCC 4 cut(s) 310, 481, 610, 947
Eco52I CGGCCG 1 cut(s) 908
Eco53kI GAGCTC 1 cut(s) 229
Eco57I CTGAAG 2 cut(s) 41, 68
EcoICRI GAGCTC 1 cut(s) 229
EcoNI CCTNNNNNAGG 1 cut(s) 618
EcoO109I RGGNCCY 1 cut(s) 310
EcoRI GAATTC 1 cut(s) 785
EcoRII CCWGG 1 cut(s) 643
EcoT14I CCWWGG 1 cut(s) 997
EcoT38I GRGCYC 1 cut(s) 231
ErhI CCWWGG 1 cut(s) 997
FaeI CATG 4 cut(s) 235, 523, 784, 1001
FaiI YATR 9 cut(s) 108, 233, 521, 754, 782, 999, 1073, 1075, 1081
FatI CATG 4 cut(s) 231, 519, 780, 997
FauI CCCGC 1 cut(s) 1053
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 7 cut(s) 178, 181, 197, 492, 905, 908, 911
FokI GGATG 1 cut(s) 707
FriOI GRGCYC 1 cut(s) 231
Fsp4HI GCNGC 7 cut(s) 178, 181, 197, 492, 905, 908, 911
FspBI CTAG 2 cut(s) 23, 386
GlaI GCGC 2 cut(s) 94, 964
GluI GCNGC 7 cut(s) 178, 181, 197, 492, 905, 908, 911
GsuI CTGGAG 1 cut(s) 987
HaeIII GGCC 1 cut(s) 910
HapII CCGG 6 cut(s) 380, 442, 449, 462, 479, 608
HgaI GACGC 1 cut(s) 892
HhaI GCGC 2 cut(s) 95, 965
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 4 cut(s) 235, 523, 784, 1001
Hin6I GCGC 2 cut(s) 93, 963
HinP1I GCGC 2 cut(s) 93, 963
HincII GTYRAC 1 cut(s) 268
HindII GTYRAC 1 cut(s) 268
HindIII AAGCTT 1 cut(s) 836
HinfI GANTC 5 cut(s) 251, 389, 458, 656, 1031
HpaII CCGG 6 cut(s) 380, 442, 449, 462, 479, 608
HphI GGTGA 2 cut(s) 73, 272
Hpy166II GTNNAC 3 cut(s) 268, 404, 641
Hpy188I TCNGA 7 cut(s) 100, 256, 394, 421, 725, 791, 943
Hpy188III TCNNGA 3 cut(s) 232, 362, 890
Hpy8I GTNNAC 3 cut(s) 268, 404, 641
Hpy99I CGWCG 3 cut(s) 224, 272, 710
HpyAV CCTTC 4 cut(s) 128, 295, 692, 712
HpyCH4IV ACGT 1 cut(s) 219
HpyCH4V TGCA 5 cut(s) 180, 714, 778, 819, 920
HpyF10VI GCNNNNNNNGC 4 cut(s) 169, 892, 901, 910
HpyF3I CTNAG 7 cut(s) 130, 173, 255, 324, 367, 454, 834
HpySE526I ACGT 1 cut(s) 219
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 4 cut(s) 235, 523, 784, 1001
HspAI GCGC 2 cut(s) 93, 963
KpnI GGTACC 1 cut(s) 468
Kzo9I GATC 3 cut(s) 73, 934, 1011
LmnI GCTCC 4 cut(s) 604, 648, 866, 918
Lsp1109I GCAGC 4 cut(s) 164, 192, 208, 891
LweI GCATC 3 cut(s) 104, 729, 765
MaeI CTAG 2 cut(s) 23, 386
MaeII ACGT 1 cut(s) 219
MaeIII GTNAC 2 cut(s) 1004, 1052
MalI GATC 3 cut(s) 75, 936, 1013
MbiI CCGCTC 1 cut(s) 913
MboI GATC 3 cut(s) 73, 934, 1011
MboII GAAGA 6 cut(s) 40, 47, 74, 463, 641, 817
MhlI GDGCHC 2 cut(s) 231, 1046
MluCI AATT 4 cut(s) 140, 335, 524, 785
MlyI GAGTC 3 cut(s) 398, 665, 1040
MmeI TCCRAC 2 cut(s) 372, 912
MroXI GAANNNNTTC 1 cut(s) 117
MseI TTAA 1 cut(s) 333
MspA1I CMGCKG 1 cut(s) 177
MspI CCGG 6 cut(s) 380, 442, 449, 462, 479, 608
MspR9I CCNGG 2 cut(s) 479, 645
MvaI CCWGG 1 cut(s) 645
MwoI GCNNNNNNNGC 4 cut(s) 169, 892, 901, 910
NciI CCSGG 1 cut(s) 479
NcoI CCATGG 1 cut(s) 997
NdeII GATC 3 cut(s) 73, 934, 1011
NlaIII CATG 4 cut(s) 235, 523, 784, 1001
NlaIV GGNNCC 5 cut(s) 312, 466, 606, 612, 828
NmuCI GTSAC 1 cut(s) 1004
NotI GCGGCCGC 1 cut(s) 908
NspV TTCGAA 1 cut(s) 627
PagI TCATGA 1 cut(s) 231
PcsI WCGNNNNNNNCGW 1 cut(s) 691
PdmI GAANNNNTTC 1 cut(s) 117
PfeI GAWTC 2 cut(s) 251, 458
PinAI ACCGGT 1 cut(s) 379
PkrI GCNGC 7 cut(s) 179, 182, 198, 493, 906, 909, 912
PleI GAGTC 3 cut(s) 397, 664, 1039
PpsI GAGTC 3 cut(s) 397, 664, 1039
PpuMI RGGWCCY 1 cut(s) 310
Psp124BI GAGCTC 1 cut(s) 231
Psp5II RGGWCCY 1 cut(s) 310
Psp6I CCWGG 1 cut(s) 643
PspGI CCWGG 1 cut(s) 643
PspN4I GGNNCC 5 cut(s) 312, 466, 606, 612, 828
PspPI GGNCC 4 cut(s) 310, 481, 610, 947
PspPPI RGGWCCY 1 cut(s) 310
PstI CTGCAG 1 cut(s) 182
PstNI CAGNNNCTG 1 cut(s) 254
PvuII CAGCTG 1 cut(s) 177
RsaI GTAC 3 cut(s) 384, 466, 518
RsaNI GTAC 3 cut(s) 383, 465, 517
SacI GAGCTC 1 cut(s) 231
SalI GTCGAC 1 cut(s) 266
SaqAI TTAA 1 cut(s) 333
SatI GCNGC 7 cut(s) 178, 181, 197, 492, 905, 908, 911
Sau3AI GATC 3 cut(s) 73, 934, 1011
Sau96I GGNCC 4 cut(s) 310, 481, 610, 947
SchI GAGTC 3 cut(s) 398, 665, 1040
ScrFI CCNGG 2 cut(s) 479, 645
SduI GDGCHC 2 cut(s) 231, 1046
SfaNI GCATC 3 cut(s) 104, 729, 765
SfcI CTRYAG 1 cut(s) 178
SfuI TTCGAA 1 cut(s) 627
SinI GGWCC 4 cut(s) 310, 481, 610, 947
SpeI ACTAGT 1 cut(s) 22
Sse9I AATT 4 cut(s) 140, 335, 524, 785
SsiI CCGC 5 cut(s) 492, 602, 907, 911, 1046
SspI AATATT 1 cut(s) 568
SspMI CTAG 2 cut(s) 23, 386
SstI GAGCTC 1 cut(s) 231
StyD4I CCNGG 2 cut(s) 477, 643
StyI CCWWGG 1 cut(s) 997
TaiI ACGT 1 cut(s) 222
TaqI TCGA 9 cut(s) 76, 222, 267, 474, 627, 685, 705, 759, 773
TasI AATT 4 cut(s) 140, 335, 524, 785
TatI WGTACW 1 cut(s) 516
TauI GCSGC 3 cut(s) 494, 910, 913
TfiI GAWTC 2 cut(s) 251, 458
Tru1I TTAA 1 cut(s) 333
Tru9I TTAA 1 cut(s) 333
TscAI CASTG 2 cut(s) 190, 1055
TseFI GTSAC 1 cut(s) 1004
TseI GCWGC 4 cut(s) 177, 180, 196, 904
Tsp45I GTSAC 1 cut(s) 1004
TspDTI ATGAA 5 cut(s) 17, 26, 536, 594, 832
TspGWI ACGGA 1 cut(s) 939
TspRI CASTG 2 cut(s) 190, 1055
VpaK11BI GGWCC 4 cut(s) 310, 481, 610, 947
XagI CCTNNNNNAGG 1 cut(s) 618
XapI RAATTY 2 cut(s) 140, 785
XmiI GTMKAC 1 cut(s) 267
XmnI GAANNNNTTC 1 cut(s) 117
XspI CTAG 2 cut(s) 23, 386
ZraI GACGTC 1 cut(s) 220
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.