MD02G1269400.v1.1

SPT2 chromatin protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0003861
Physical Location & Seq
Reverse (-)
32378207 .. 32381871
3665 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1269400.v1.1.491

Sequence Viewer

Length: 1299 bp
ATGCGGGGATATGGTAGAGATGAATGCTATCAATCAGTCAAAGAATATGATGATTATGAGGACGAGGGATATGGGGAAGCGGAGGATGGAAAAAAAGATACAGATCGCACCAACGTAACTGTGGCACGTCTTGATTTTAGACAGTGCTTGAAAGAACAACATAGAAAGAAGTTGGAGATAGATGGTGGTTCTTCTCAAGCCAAGTCCTATGGAAAGAAGAAGCTACCCTATGATAATTACGGATCTTTTTTTGGCCCATATCAACCAGTTATCTCTCAGAGAGTAATTCAAGTGACCCAGCAACTCTCATCTAGGGTTAATCCCCTTCAACTTGACAAGAAAAGCTCTGGATCGACATCTTCTGGATCAAAATCTGTATCATATGGCCAGAAACCCAAAGTCATGAATGAGAAAAAAACTAAAGTTCAAATGATTAGGGAAATGAGAGATTACTCACAATTTTTCTCTAAGGATGCAGACTGTCCAGCTCCTGCCAAGGATTGTCCCCCTCAAAATGTCTCGCTTCCAAACTCTGGGGTGAGGTCAAATCATATGGCGATGAAAATCAAACAGTCACTGGAAAGTAATGGAAGGAAACCGCCGACTATGAAAGAACAGATGCATTCTGAAGTTGGGTCCACTAAGTTAATTTCTGCACGTAGAAGAATTGATTCAAAATCAATGGGCACTAGGAAGCAGCTTGAAAGCAATGAAAATGGGTCGGGCCGGCCTTTAGGGTCAAAAGGGCTTCCTTTGAAGATGTCTGCTTCAACTACTGAGAGGAAGACTTCTGCACCTGGTGTGAAGAATTCCACCACGTCTGGTTTGCACAAACCACTCCCTCCAAAGTTGCAGTCATCTGTTTCAAGGCAGCACCTGACACAAAAAGCGGAAGTACATATGCCGTATACTAAGCCCAAAGCAAGACAGTCGACAGGATTGTCAAAACCTCAGATGAGCATGCAACAATTGCAAAAGCAAACCTCACCCCGTACTATGTATGATAATCGTCCCAAGAAAAAAAGACGGTGTTCCGATGATCAGTATGACCCTGAGGCCGGGAATGTTCGTGATGTGATAAGAAAAATGTTTCGTTATAATCCCGATAGATTTGCTGGTGATGCTGAAGACGATAGTGATATGGAGGCAGGTTTTGAAGATATTCAGAGGGAAGAGAGAAGAAGCGCGCTAATTGCGAGAAGAGAGGATGAAGAGCAAGCTAGGTTGATAGAAGCCGAAGAAAGAAAGGAACGGATGGCCAGGTTGAGAAAGCAAAAGAAGCGTCAGCTGGTTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPT2

Protein Analysis

433

Amino Acids

49.23

Weight (kDa)

9.74

Isoelectric Point (pI)

56.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPT2 PF08243 322 - 419 4e-16 SPT2 chromatin protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1098
AasI GACNNNNNNGTC 1 cut(s) 940
Acc36I ACCTGC 1 cut(s) 1139
AccB7I CCANNNNNTGG 1 cut(s) 533
AccI GTMKAC 2 cut(s) 908, 932
AccII CGCG 1 cut(s) 1187
AciI CCGC 4 cut(s) 4, 80, 599, 890
AclWI GGATC 3 cut(s) 250, 358, 373
AcoI YGGCCR 2 cut(s) 385, 1257
AcsI RAATTY 1 cut(s) 808
AcuI CTGAAG 2 cut(s) 648, 1146
AdeI CACNNNGTG 1 cut(s) 800
AfaI GTAC 2 cut(s) 897, 994
AfiI CCNNNNNNNGG 2 cut(s) 533, 1058
AjiI CACGTC 2 cut(s) 128, 819
AjnI CCWGG 2 cut(s) 796, 1259
AluBI AGCT 6 cut(s) 223, 345, 488, 700, 1220, 1288
AluI AGCT 6 cut(s) 223, 345, 488, 700, 1220, 1288
Alw26I GTCTC 1 cut(s) 523
AlwI GGATC 3 cut(s) 250, 358, 373
AlwNI CAGNNNCTG 3 cut(s) 491, 577, 877
AoxI GGCC 6 cut(s) 253, 385, 724, 728, 1056, 1257
ApeKI GCWGC 2 cut(s) 697, 871
ApoI RAATTY 1 cut(s) 808
ArsI GACNNNNNNTTYG 4 cut(s) 839, 871, 1267, 1299
Asp700I GAANNNNTTC 2 cut(s) 670, 1161
AspLEI GCGC 2 cut(s) 1187, 1189
AspS9I GGNCC 3 cut(s) 254, 636, 724
AsuC2I CCSGG 1 cut(s) 1060
AsuHPI GGTGA 3 cut(s) 550, 978, 1130
AvaII GGWCC 1 cut(s) 636
AxyI CCTNAGG 1 cut(s) 1053
BaeGI GKGCMC 1 cut(s) 689
BalI TGGCCA 2 cut(s) 387, 1259
BbsI GAAGAC 2 cut(s) 791, 1134
BbvI GCAGC 2 cut(s) 709, 883
BccI CCATC 3 cut(s) 80, 176, 1249
BceAI ACGGC 1 cut(s) 889
BcgI CGANNNNNNTGC 4 cut(s) 912, 946, 1094, 1128
BciT130I CCWGG 2 cut(s) 798, 1261
BclI TGATCA 1 cut(s) 1039
BcnI CCSGG 1 cut(s) 1060
BcoDI GTCTC 1 cut(s) 523
BfaI CTAG 3 cut(s) 312, 690, 1221
BfuAI ACCTGC 1 cut(s) 1139
BisI GCNGC 2 cut(s) 698, 872
BlsI GCNGC 2 cut(s) 699, 873
Bme1390I CCNGG 3 cut(s) 798, 1060, 1261
Bme18I GGWCC 1 cut(s) 636
BmgBI CACGTC 2 cut(s) 128, 819
BmgT120I GGNCC 3 cut(s) 254, 636, 724
BmiI GGNNCC 1 cut(s) 637
BmrFI CCNGG 3 cut(s) 798, 1060, 1261
BmsI GCATC 3 cut(s) 463, 609, 1111
BpiI GAAGAC 2 cut(s) 791, 1134
BpuEI CTTGAG 1 cut(s) 180
BpuMI CCSGG 1 cut(s) 1060
BsaAI YACGTR 1 cut(s) 659
BsaBI GATNNNNATC 4 cut(s) 102, 355, 370, 563
BsaJI CCNNGG 1 cut(s) 495
Bsc4I CCNNNNNNNGG 2 cut(s) 533, 1058
Bse118I RCCGGY 1 cut(s) 726
Bse1I ACTGG 2 cut(s) 266, 582
Bse21I CCTNAGG 1 cut(s) 1053
Bse3DI GCAATG 1 cut(s) 715
Bse8I GATNNNNATC 4 cut(s) 102, 355, 370, 563
BseBI CCWGG 2 cut(s) 798, 1261
BseDI CCNNGG 1 cut(s) 495
BseGI GGATG 4 cut(s) 91, 478, 1213, 1260
BseJI GATNNNNATC 4 cut(s) 102, 355, 370, 563
BseLI CCNNNNNNNGG 2 cut(s) 533, 1058
BseMI GCAATG 1 cut(s) 715
BseMII CTCAG 4 cut(s) 290, 768, 965, 1044
BseNI ACTGG 2 cut(s) 266, 582
BsePI GCGCGC 1 cut(s) 1185
BseSI GKGCMC 1 cut(s) 689
BseXI GCAGC 2 cut(s) 709, 883
BseYI CCCAGC 1 cut(s) 297
BsgI GTGCAG 2 cut(s) 639, 777
Bsh1236I CGCG 1 cut(s) 1187
BshFI GGCC 6 cut(s) 255, 387, 726, 730, 1058, 1259
BsiSI CCGG 2 cut(s) 727, 1059
BslFI GGGAC 2 cut(s) 489, 996
BslI CCNNNNNNNGG 2 cut(s) 533, 1058
BsmAI GTCTC 1 cut(s) 523
BsmFI GGGAC 2 cut(s) 489, 996
BsmI GAATGC 2 cut(s) 29, 622
BsnI GGCC 6 cut(s) 255, 387, 726, 730, 1058, 1259
Bsp1286I GDGCHC 1 cut(s) 689
Bsp143I GATC 5 cut(s) 103, 242, 350, 365, 1039
BspACI CCGC 4 cut(s) 4, 80, 599, 890
BspANI GGCC 6 cut(s) 255, 387, 726, 730, 1058, 1259
BspCNI CTCAG 4 cut(s) 289, 769, 964, 1045
BspFNI CGCG 1 cut(s) 1187
BspHI TCATGA 1 cut(s) 402
BspLI GGNNCC 1 cut(s) 637
BspMI ACCTGC 1 cut(s) 1139
BspPI GGATC 3 cut(s) 250, 358, 373
BspQI GCTCTTC 1 cut(s) 1206
BsrDI GCAATG 1 cut(s) 715
BsrFI RCCGGY 1 cut(s) 726
BsrI ACTGG 2 cut(s) 266, 582
BssAI RCCGGY 1 cut(s) 726
BssECI CCNNGG 1 cut(s) 495
BssHII GCGCGC 1 cut(s) 1185
BssMI GATC 5 cut(s) 103, 242, 350, 365, 1039
BssNAI GTATAC 1 cut(s) 909
BssT1I CCWWGG 1 cut(s) 495
Bst1107I GTATAC 1 cut(s) 909
Bst2UI CCWGG 2 cut(s) 798, 1261
Bst4CI ACNGT 6 cut(s) 121, 144, 482, 573, 930, 1029
Bst6I CTCTTC 3 cut(s) 1167, 1195, 1206
BstAPI GCANNNNNTGC 1 cut(s) 970
BstBAI YACGTR 1 cut(s) 659
BstC8I GCNNGC 4 cut(s) 728, 962, 1187, 1218
BstDEI CTNAG 7 cut(s) 276, 468, 642, 777, 912, 951, 1053
BstF5I GGATG 4 cut(s) 91, 478, 1213, 1260
BstFNI CGCG 1 cut(s) 1187
BstHHI GCGC 2 cut(s) 1187, 1189
BstKTI GATC 5 cut(s) 106, 245, 353, 368, 1042
BstMAI GTCTC 1 cut(s) 523
BstMBI GATC 5 cut(s) 103, 242, 350, 365, 1039
BstMWI GCNNNNNNNGC 4 cut(s) 970, 1121, 1193, 1279
BstNI CCWGG 2 cut(s) 798, 1261
BstNSI RCATGY 1 cut(s) 964
BstSCI CCNGG 3 cut(s) 796, 1058, 1259
BstSLI GKGCMC 1 cut(s) 689
BstUI CGCG 1 cut(s) 1187
BstV1I GCAGC 2 cut(s) 709, 883
BstV2I GAAGAC 2 cut(s) 791, 1134
BstX2I RGATCY 1 cut(s) 242
BstYI RGATCY 1 cut(s) 242
BstZ17I GTATAC 1 cut(s) 909
Bsu36I CCTNAGG 1 cut(s) 1053
BsuRI GGCC 6 cut(s) 255, 387, 726, 730, 1058, 1259
BtgZI GCGATG 1 cut(s) 572
BtrI CACGTC 2 cut(s) 128, 819
BtsCI GGATG 4 cut(s) 91, 478, 1213, 1260
BtsIMutI CAGTG 2 cut(s) 149, 575
BveI ACCTGC 1 cut(s) 1139
Cac8I GCNNGC 4 cut(s) 728, 962, 1187, 1218
CaiI CAGNNNCTG 3 cut(s) 491, 577, 877
CciI TCATGA 1 cut(s) 402
CfoI GCGC 2 cut(s) 1187, 1189
Cfr10I RCCGGY 1 cut(s) 726
Cfr13I GGNCC 3 cut(s) 254, 636, 724
CseI GACGC 1 cut(s) 1271
CsiI ACCWGGT 1 cut(s) 796
Csp6I GTAC 2 cut(s) 896, 993
CviAII CATG 2 cut(s) 403, 961
CviQI GTAC 2 cut(s) 896, 993
DdeI CTNAG 7 cut(s) 276, 468, 642, 777, 912, 951, 1053
DpnI GATC 5 cut(s) 105, 244, 352, 367, 1041
DpnII GATC 5 cut(s) 103, 242, 350, 365, 1039
DraIII CACNNNGTG 1 cut(s) 800
DrdI GACNNNNNNGTC 1 cut(s) 940
DseDI GACNNNNNNGTC 1 cut(s) 940
EaeI YGGCCR 2 cut(s) 385, 1257
Eam1104I CTCTTC 3 cut(s) 1167, 1195, 1206
EarI CTCTTC 3 cut(s) 1167, 1195, 1206
Eco130I CCWWGG 1 cut(s) 495
Eco47I GGWCC 1 cut(s) 636
Eco57I CTGAAG 2 cut(s) 648, 1146
Eco81I CCTNAGG 1 cut(s) 1053
EcoRI GAATTC 1 cut(s) 808
EcoRII CCWGG 2 cut(s) 796, 1259
EcoT14I CCWWGG 1 cut(s) 495
EcoT22I ATGCAT 1 cut(s) 624
ErhI CCWWGG 1 cut(s) 495
FaeI CATG 2 cut(s) 406, 964
FaqI GGGAC 2 cut(s) 489, 996
FatI CATG 2 cut(s) 402, 960
FauNDI CATATG 3 cut(s) 382, 552, 900
FbaI TGATCA 1 cut(s) 1039
FblI GTMKAC 2 cut(s) 908, 932
Fnu4HI GCNGC 2 cut(s) 698, 872
FokI GGATG 4 cut(s) 98, 485, 1220, 1267
FseI GGCCGGCC 1 cut(s) 730
Fsp4HI GCNGC 2 cut(s) 698, 872
FspBI CTAG 3 cut(s) 312, 690, 1221
GlaI GCGC 2 cut(s) 1186, 1188
GluI GCNGC 2 cut(s) 698, 872
GsaI CCCAGC 1 cut(s) 301
HaeIII GGCC 6 cut(s) 255, 387, 726, 730, 1058, 1259
HapII CCGG 2 cut(s) 727, 1059
HgaI GACGC 1 cut(s) 1271
HhaI GCGC 2 cut(s) 1187, 1189
Hin1II CATG 2 cut(s) 406, 964
Hin6I GCGC 2 cut(s) 1185, 1187
HinP1I GCGC 2 cut(s) 1185, 1187
HincII GTYRAC 1 cut(s) 933
HindII GTYRAC 1 cut(s) 933
HinfI GANTC 1 cut(s) 671
HpaII CCGG 2 cut(s) 727, 1059
HphI GGTGA 3 cut(s) 550, 978, 1130
Hpy166II GTNNAC 3 cut(s) 639, 909, 933
Hpy188I TCNGA 5 cut(s) 279, 628, 954, 1036, 1167
Hpy188III TCNNGA 6 cut(s) 131, 348, 363, 403, 1070, 1103
Hpy8I GTNNAC 3 cut(s) 639, 909, 933
HpyAV CCTTC 2 cut(s) 335, 585
HpyCH4III ACNGT 6 cut(s) 121, 144, 482, 573, 930, 1029
HpyCH4IV ACGT 4 cut(s) 114, 127, 658, 818
HpyCH4V TGCA 8 cut(s) 476, 622, 656, 794, 829, 853, 964, 973
HpyF10VI GCNNNNNNNGC 4 cut(s) 970, 1121, 1193, 1279
HpyF3I CTNAG 7 cut(s) 276, 468, 642, 777, 912, 951, 1053
HpySE526I ACGT 4 cut(s) 114, 127, 658, 818
Hsp92II CATG 2 cut(s) 406, 964
HspAI GCGC 2 cut(s) 1185, 1187
KroI GCCGGC 1 cut(s) 726
KroNI GCCGGC 1 cut(s) 728
Ksp22I TGATCA 1 cut(s) 1039
Kzo9I GATC 5 cut(s) 103, 242, 350, 365, 1039
LguI GCTCTTC 1 cut(s) 1206
LmnI GCTCC 1 cut(s) 493
Lsp1109I GCAGC 2 cut(s) 709, 883
LweI GCATC 3 cut(s) 463, 609, 1111
MabI ACCWGGT 1 cut(s) 796
MaeI CTAG 3 cut(s) 312, 690, 1221
MaeII ACGT 4 cut(s) 114, 127, 658, 818
MaeIII GTNAC 3 cut(s) 115, 292, 573
MalI GATC 5 cut(s) 105, 244, 352, 367, 1041
MboI GATC 5 cut(s) 103, 242, 350, 365, 1039
MfeI CAATTG 1 cut(s) 968
MflI RGATCY 1 cut(s) 242
MhlI GDGCHC 1 cut(s) 689
MlsI TGGCCA 2 cut(s) 387, 1259
MluCI AATT 8 cut(s) 235, 285, 458, 648, 666, 808, 968, 1191
MluNI TGGCCA 2 cut(s) 387, 1259
MmeI TCCRAC 1 cut(s) 153
Mox20I TGGCCA 2 cut(s) 387, 1259
Mph1103I ATGCAT 1 cut(s) 624
MroNI GCCGGC 1 cut(s) 726
MroXI GAANNNNTTC 2 cut(s) 670, 1161
MscI TGGCCA 2 cut(s) 387, 1259
MseI TTAA 2 cut(s) 318, 647
Msp20I TGGCCA 2 cut(s) 387, 1259
MspA1I CMGCKG 1 cut(s) 1288
MspI CCGG 2 cut(s) 727, 1059
MspR9I CCNGG 3 cut(s) 798, 1060, 1261
MunI CAATTG 1 cut(s) 968
Mva1269I GAATGC 2 cut(s) 29, 622
MvaI CCWGG 2 cut(s) 798, 1261
MvnI CGCG 1 cut(s) 1187
MwoI GCNNNNNNNGC 4 cut(s) 970, 1121, 1193, 1279
NaeI GCCGGC 1 cut(s) 728
NciI CCSGG 1 cut(s) 1060
NdeI CATATG 3 cut(s) 382, 552, 900
NdeII GATC 5 cut(s) 103, 242, 350, 365, 1039
NgoMIV GCCGGC 1 cut(s) 726
NlaIII CATG 2 cut(s) 406, 964
NlaIV GGNNCC 1 cut(s) 637
NmuCI GTSAC 2 cut(s) 292, 573
NsiI ATGCAT 1 cut(s) 624
NspI RCATGY 1 cut(s) 964
PaeI GCATGC 1 cut(s) 964
PagI TCATGA 1 cut(s) 402
PauI GCGCGC 1 cut(s) 1185
PciSI GCTCTTC 1 cut(s) 1206
PctI GAATGC 2 cut(s) 29, 622
PdiI GCCGGC 1 cut(s) 728
PdmI GAANNNNTTC 2 cut(s) 670, 1161
PfeI GAWTC 1 cut(s) 671
PflMI CCANNNNNTGG 1 cut(s) 533
PkrI GCNGC 2 cut(s) 699, 873
Ppu21I YACGTR 1 cut(s) 659
PsiI TTATAA 1 cut(s) 1098
Psp6I CCWGG 2 cut(s) 796, 1259
PspFI CCCAGC 1 cut(s) 297
PspGI CCWGG 2 cut(s) 796, 1259
PspN4I GGNNCC 1 cut(s) 637
PspPI GGNCC 3 cut(s) 254, 636, 724
PstNI CAGNNNCTG 3 cut(s) 491, 577, 877
PsuI RGATCY 1 cut(s) 242
PteI GCGCGC 1 cut(s) 1185
PvuII CAGCTG 1 cut(s) 1288
RigI GGCCGGCC 1 cut(s) 730
RsaI GTAC 2 cut(s) 897, 994
RsaNI GTAC 2 cut(s) 896, 993
SalI GTCGAC 1 cut(s) 931
SapI GCTCTTC 1 cut(s) 1206
SaqAI TTAA 2 cut(s) 318, 647
SatI GCNGC 2 cut(s) 698, 872
Sau3AI GATC 5 cut(s) 103, 242, 350, 365, 1039
Sau96I GGNCC 3 cut(s) 254, 636, 724
ScrFI CCNGG 3 cut(s) 798, 1060, 1261
SduI GDGCHC 1 cut(s) 689
SexAI ACCWGGT 1 cut(s) 796
SfaNI GCATC 3 cut(s) 463, 609, 1111
SinI GGWCC 1 cut(s) 636
SmlI CTYRAG 1 cut(s) 195
SmoI CTYRAG 1 cut(s) 195
SphI GCATGC 1 cut(s) 964
Sse9I AATT 8 cut(s) 235, 285, 458, 648, 666, 808, 968, 1191
SsiI CCGC 4 cut(s) 4, 80, 599, 890
SspMI CTAG 3 cut(s) 312, 690, 1221
StyD4I CCNGG 3 cut(s) 796, 1058, 1259
StyI CCWWGG 1 cut(s) 495
TaaI ACNGT 6 cut(s) 121, 144, 482, 573, 930, 1029
TaiI ACGT 4 cut(s) 117, 130, 661, 821
TaqI TCGA 2 cut(s) 353, 932
TasI AATT 8 cut(s) 235, 285, 458, 648, 666, 808, 968, 1191
TatI WGTACW 1 cut(s) 895
TfiI GAWTC 1 cut(s) 671
Tru1I TTAA 2 cut(s) 318, 647
Tru9I TTAA 2 cut(s) 318, 647
TscAI CASTG 2 cut(s) 149, 582
TseFI GTSAC 2 cut(s) 292, 573
TseI GCWGC 2 cut(s) 697, 871
Tsp45I GTSAC 2 cut(s) 292, 573
TspDTI ATGAA 7 cut(s) 36, 419, 575, 623, 726, 1224, 1283
TspGWI ACGGA 2 cut(s) 255, 1267
TspRI CASTG 2 cut(s) 149, 582
Van91I CCANNNNNTGG 1 cut(s) 533
VpaK11BI GGWCC 1 cut(s) 636
XapI RAATTY 1 cut(s) 808
XceI RCATGY 1 cut(s) 964
XcmI CCANNNNNNNNNTGG 1 cut(s) 118
XmiI GTMKAC 2 cut(s) 908, 932
XmnI GAANNNNTTC 2 cut(s) 670, 1161
XspI CTAG 3 cut(s) 312, 690, 1221
Zsp2I ATGCAT 1 cut(s) 624
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.