MD16G1281700.v1.1

SPT2 chromatin protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0003861
Physical Location & Seq
Forward (+)
38405789 .. 38411319
5531 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1281700.v1.1.491

Sequence Viewer

Length: 1509 bp
ATGCGGGGATATGATAGAGATGATTATGGTCAGTCAGGCGACGAATATGAGGATTATGAGGACGATGAGGATGGTGAAGAGTATGAAGAGGAAGAGGAAGATCCAAAGCCTACCAAGGAAGCATTGGCATACCTTGAGTTGAGACAACGCTTGAAAGAACAATTACGAAAGAAGAAAAGTGGTTCTTCTTTGGCCAACCCCAGTAATAAAAAGAAGAAGCTCCCCTATGACAATTTTGGATCTTTCTTTGGCCCATCTCAACCAGTTATTGCTGAGAGAGTGATTCAAGAAAGCAAATCGTTGTTAGAAACCAAGCACCTAACATCCAGTGCTACCAACTCTGTCCATCATAACAAGAAGAGCTCTGGATCAACCTCTGCTGGATCGAAATATGTATCAAATGATCAGAAACCCAAAGTCATTAATGAGGTTAAGAGTAAAGTACAAAAGATCAAGGATACAAGAGATTACTCGTTTTTACTTTCTGACGATGCAGAGCCCCCAGCCTCTACAAAGGATCGACCACCTCAAAATGTCTCTGTTCCAAATTCTGGTGTGCGGTCTAGTCAAATGGCGACAAATAATGGCAGACATATACCTTCATCAAATAATGGCAGACATATACCTTCATCAAATAATGCCAGACCTGTACCTTCCTCAAATAATGGAAGACATGTACCTTCGGCAAATAATGGCAGACATGTACTTTCAGCAAATAATGGTAGACATATACCTTCAGCAAATAATGGCAGACATGTACCTTCAGCAAATGATGGTAGAGTTGTACCTGGCCGTCGTGATGAAAGGAAACTGGTATCTATGAATGGACAGATGCATTCTAAAGTGGGGCCCAATAAGTTGAGTTCTGCCAGTAGACGACCCGATTCAACATCAATGGACTCGAGAAGACAGCTTGGTAGCATAGGTGGAAATGGGCCTGGCCGGCCTCTAGAGACAAAAAGATTACCGTCTAAGATGCCTGCTTCAACCACTGAGAGGAAAGCTTCTACACCAGGCCTAAAGAATCCCATGTCTGGTGTGCCCAGACCACCTCTTTCAAAGTTGCAGTCATCTATTCCAAGGCAGCAGTTGCAACAAAAAAAAGAAGTACGAGAATCTAATAAGCCCAAAGTGTTACTGAAACAGTCATCAGGATTGTCAAGACCTCAGCACATAAACAAGCCACAAATGCAAAGGCAAATCTCGTCACGTCCTATGTCTCAAGAGCATCCTCCCAAAAAGAAGCCTATGAGACGGCACCCAGATGATGATGAACCGGGTCTGGATTTTAGAAGTGAGATCAGAAAAATGTTTCGTCAACCAGAAAGATACGCTAGTGACGACGACGATAGTGATATGGAGGCGAACTTTGAAGATATTATGAAGGAGGAGAGACGAAGTGCGCTAATCGCAAGAAGGGAGGACGAAGAGCAGGCTAGGTTGATAGAAGAAGAAGAAAGGAGGGAACGGATGGCTAAACAAAATCGCAAGCGTAAGCTCGGTCATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPT2

Protein Analysis

503

Amino Acids

56.6

Weight (kDa)

9.79

Isoelectric Point (pI)

71.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPT2 PF08243 399 - 493 1.1e-15 SPT2 chromatin protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1257
AccB7I CCANNNNNTGG 1 cut(s) 551
AccI GTMKAC 2 cut(s) 724, 874
AciI CCGC 2 cut(s) 4, 559
AclWI GGATC 5 cut(s) 95, 247, 376, 391, 525
AcoI YGGCCR 3 cut(s) 192, 790, 940
AcsI RAATTY 1 cut(s) 547
AcuI CTGAAG 2 cut(s) 720, 747
AfaI GTAC 7 cut(s) 444, 651, 678, 705, 759, 786, 1110
AfiI CCNNNNNNNGG 3 cut(s) 551, 996, 1034
AflIII ACRYGT 3 cut(s) 673, 700, 754
AgsI TTSAA 6 cut(s) 154, 287, 888, 987, 1059, 1373
AjiI CACGTC 1 cut(s) 1211
AjnI CCWGG 3 cut(s) 787, 937, 1012
AluBI AGCT 5 cut(s) 220, 363, 913, 1004, 1498
AluI AGCT 5 cut(s) 220, 363, 913, 1004, 1498
Alw21I GWGCWC 1 cut(s) 365
Alw26I GTCTC 6 cut(s) 136, 541, 947, 1224, 1246, 1387
AlwI GGATC 5 cut(s) 95, 247, 376, 391, 525
Ama87I CYCGRG 1 cut(s) 901
AoxI GGCC 8 cut(s) 192, 250, 790, 848, 935, 940, 944, 1015
ApaI GGGCCC 1 cut(s) 852
ApeKI GCWGC 1 cut(s) 1084
ApoI RAATTY 1 cut(s) 547
ArsI GACNNNNNNTTYG 2 cut(s) 1052, 1084
AseI ATTAAT 1 cut(s) 423
AspLEI GCGC 1 cut(s) 1405
AspS9I GGNCC 4 cut(s) 251, 848, 849, 935
AsuC2I CCSGG 1 cut(s) 1278
AsuHPI GGTGA 1 cut(s) 86
AvaI CYCGRG 1 cut(s) 901
BaeGI GKGCMC 2 cut(s) 852, 1044
BaeI ACNNNNGTAYC 2 cut(s) 1321, 1354
BalI TGGCCA 1 cut(s) 194
BanI GGYRCC 1 cut(s) 1257
BanII GRGCYC 3 cut(s) 365, 501, 852
BbsI GAAGAC 2 cut(s) 676, 913
Bbv12I GWGCWC 1 cut(s) 365
BbvCI CCTCAGC 1 cut(s) 1167
BbvI GCAGC 1 cut(s) 1096
BccI CCATC 5 cut(s) 65, 262, 354, 767, 1465
BceAI ACGGC 2 cut(s) 777, 1271
BciT130I CCWGG 3 cut(s) 789, 939, 1014
BciVI GTATCC 1 cut(s) 451
BclI TGATCA 1 cut(s) 403
BcnI CCSGG 1 cut(s) 1278
BcoDI GTCTC 6 cut(s) 136, 541, 947, 1224, 1246, 1387
BfaI CTAG 4 cut(s) 564, 950, 1335, 1437
BfuI GTATCC 1 cut(s) 451
BglI GCCNNNNNGGC 1 cut(s) 943
BisI GCNGC 1 cut(s) 1085
BlsI GCNGC 1 cut(s) 1086
Bme1390I CCNGG 4 cut(s) 789, 939, 1014, 1278
BmeT110I CYCGRG 1 cut(s) 901
BmgBI CACGTC 1 cut(s) 1211
BmgT120I GGNCC 4 cut(s) 251, 848, 849, 935
BmiI GGNNCC 3 cut(s) 849, 850, 1259
BmrFI CCNGG 4 cut(s) 789, 939, 1014, 1278
BmrI ACTGGG 1 cut(s) 195
BmsI GCATC 4 cut(s) 481, 822, 966, 1237
BmuI ACTGGG 1 cut(s) 195
BpiI GAAGAC 2 cut(s) 676, 913
Bpu10I CCTNAGC 1 cut(s) 1167
BpuEI CTTGAG 2 cut(s) 155, 1206
BpuMI CCSGG 1 cut(s) 1278
BsaJI CCNNGG 2 cut(s) 114, 1079
Bsc4I CCNNNNNNNGG 3 cut(s) 551, 996, 1034
Bse118I RCCGGY 1 cut(s) 942
Bse1I ACTGG 5 cut(s) 201, 263, 327, 816, 870
BseBI CCWGG 3 cut(s) 789, 939, 1014
BseDI CCNNGG 2 cut(s) 114, 1079
BseGI GGATG 4 cut(s) 76, 323, 1228, 1476
BseLI CCNNNNNNNGG 3 cut(s) 551, 996, 1034
BseMII CTCAG 3 cut(s) 264, 984, 1181
BseNI ACTGG 5 cut(s) 201, 263, 327, 816, 870
BseRI GAGGAG 1 cut(s) 1403
BseSI GKGCMC 2 cut(s) 852, 1044
BseXI GCAGC 1 cut(s) 1096
BseYI CCCAGC 1 cut(s) 502
BshFI GGCC 8 cut(s) 194, 252, 792, 850, 937, 942, 946, 1017
BshNI GGYRCC 1 cut(s) 1257
BsiHKAI GWGCWC 1 cut(s) 365
BsiHKCI CYCGRG 1 cut(s) 901
BsiSI CCGG 2 cut(s) 943, 1277
BslI CCNNNNNNNGG 3 cut(s) 551, 996, 1034
BsmAI GTCTC 6 cut(s) 136, 541, 947, 1224, 1246, 1387
BsmBI CGTCTC 2 cut(s) 1246, 1387
BsmI GAATGC 1 cut(s) 835
BsnI GGCC 8 cut(s) 194, 252, 792, 850, 937, 942, 946, 1017
BsoBI CYCGRG 1 cut(s) 901
Bsp120I GGGCCC 1 cut(s) 848
Bsp1286I GDGCHC 4 cut(s) 365, 501, 852, 1044
Bsp143I GATC 8 cut(s) 100, 239, 368, 383, 403, 450, 517, 1299
BspACI CCGC 2 cut(s) 4, 559
BspANI GGCC 8 cut(s) 194, 252, 792, 850, 937, 942, 946, 1017
BspCNI CTCAG 3 cut(s) 265, 985, 1180
BspLI GGNNCC 3 cut(s) 849, 850, 1259
BspPI GGATC 5 cut(s) 95, 247, 376, 391, 525
BspQI GCTCTTC 2 cut(s) 353, 1422
BspT107I GGYRCC 1 cut(s) 1257
BsrFI RCCGGY 1 cut(s) 942
BsrI ACTGG 5 cut(s) 201, 263, 327, 816, 870
BssAI RCCGGY 1 cut(s) 942
BssECI CCNNGG 2 cut(s) 114, 1079
BssMI GATC 8 cut(s) 100, 239, 368, 383, 403, 450, 517, 1299
BssT1I CCWWGG 2 cut(s) 114, 1079
Bst2UI CCWGG 3 cut(s) 789, 939, 1014
Bst4CI ACNGT 2 cut(s) 969, 1146
Bst6I CTCTTC 5 cut(s) 72, 81, 87, 353, 1422
BstAPI GCANNNNNTGC 1 cut(s) 1090
BstC8I GCNNGC 4 cut(s) 944, 981, 1434, 1490
BstDEI CTNAG 4 cut(s) 273, 972, 993, 1167
BstF5I GGATG 4 cut(s) 76, 323, 1228, 1476
BstHHI GCGC 1 cut(s) 1405
BstKTI GATC 8 cut(s) 103, 242, 371, 386, 406, 453, 520, 1302
BstMAI GTCTC 6 cut(s) 136, 541, 947, 1224, 1246, 1387
BstMBI GATC 8 cut(s) 100, 239, 368, 383, 403, 450, 517, 1299
BstMWI GCNNNNNNNGC 4 cut(s) 943, 1090, 1189, 1409
BstNI CCWGG 3 cut(s) 789, 939, 1014
BstNSI RCATGY 3 cut(s) 677, 704, 758
BstSCI CCNGG 4 cut(s) 787, 937, 1012, 1276
BstSLI GKGCMC 2 cut(s) 852, 1044
BstV1I GCAGC 1 cut(s) 1096
BstV2I GAAGAC 2 cut(s) 676, 913
BstX2I RGATCY 2 cut(s) 100, 239
BstYI RGATCY 2 cut(s) 100, 239
BsuI GTATCC 1 cut(s) 451
BsuRI GGCC 8 cut(s) 194, 252, 792, 850, 937, 942, 946, 1017
BtrI CACGTC 1 cut(s) 1211
BtsCI GGATG 4 cut(s) 76, 323, 1228, 1476
BtsIMutI CAGTG 2 cut(s) 334, 990
Cac8I GCNNGC 4 cut(s) 944, 981, 1434, 1490
CfoI GCGC 1 cut(s) 1405
Cfr10I RCCGGY 1 cut(s) 942
Cfr13I GGNCC 4 cut(s) 251, 848, 849, 935
Csp6I GTAC 7 cut(s) 443, 650, 677, 704, 758, 785, 1109
CviAII CATG 4 cut(s) 674, 701, 755, 1030
CviQI GTAC 7 cut(s) 443, 650, 677, 704, 758, 785, 1109
DdeI CTNAG 4 cut(s) 273, 972, 993, 1167
DpnI GATC 8 cut(s) 102, 241, 370, 385, 405, 452, 519, 1301
DpnII GATC 8 cut(s) 100, 239, 368, 383, 403, 450, 517, 1299
EaeI YGGCCR 3 cut(s) 192, 790, 940
Eam1104I CTCTTC 5 cut(s) 72, 81, 87, 353, 1422
EarI CTCTTC 5 cut(s) 72, 81, 87, 353, 1422
Ecl136II GAGCTC 1 cut(s) 363
Eco130I CCWWGG 2 cut(s) 114, 1079
Eco147I AGGCCT 1 cut(s) 1017
Eco24I GRGCYC 3 cut(s) 365, 501, 852
Eco53kI GAGCTC 1 cut(s) 363
Eco57I CTGAAG 2 cut(s) 720, 747
Eco88I CYCGRG 1 cut(s) 901
EcoICRI GAGCTC 1 cut(s) 363
EcoO109I RGGNCCY 1 cut(s) 848
EcoRII CCWGG 3 cut(s) 787, 937, 1012
EcoT14I CCWWGG 2 cut(s) 114, 1079
EcoT22I ATGCAT 1 cut(s) 837
EcoT38I GRGCYC 3 cut(s) 365, 501, 852
ErhI CCWWGG 2 cut(s) 114, 1079
Esp3I CGTCTC 2 cut(s) 1246, 1387
FaeI CATG 4 cut(s) 677, 704, 758, 1033
FalI AAGNNNNNCTT 2 cut(s) 169, 201
FatI CATG 4 cut(s) 673, 700, 754, 1029
FbaI TGATCA 1 cut(s) 403
FblI GTMKAC 2 cut(s) 724, 874
Fnu4HI GCNGC 1 cut(s) 1085
FokI GGATG 4 cut(s) 83, 310, 1215, 1483
FriOI GRGCYC 3 cut(s) 365, 501, 852
FseI GGCCGGCC 1 cut(s) 946
Fsp4HI GCNGC 1 cut(s) 1085
FspBI CTAG 4 cut(s) 564, 950, 1335, 1437
GlaI GCGC 1 cut(s) 1404
GluI GCNGC 1 cut(s) 1085
GsaI CCCAGC 1 cut(s) 506
HaeIII GGCC 8 cut(s) 194, 252, 792, 850, 937, 942, 946, 1017
HapII CCGG 2 cut(s) 943, 1277
HhaI GCGC 1 cut(s) 1405
Hin1II CATG 4 cut(s) 677, 704, 758, 1033
Hin6I GCGC 1 cut(s) 1403
HinP1I GCGC 1 cut(s) 1403
HincII GTYRAC 1 cut(s) 1319
HindII GTYRAC 1 cut(s) 1319
HindIII AAGCTT 1 cut(s) 1002
HinfI GANTC 5 cut(s) 283, 884, 899, 1024, 1115
HpaII CCGG 2 cut(s) 943, 1277
HphI GGTGA 1 cut(s) 86
Hpy166II GTNNAC 3 cut(s) 725, 875, 1319
Hpy188I TCNGA 3 cut(s) 408, 487, 1304
Hpy188III TCNNGA 9 cut(s) 287, 366, 797, 903, 950, 1152, 1161, 1223, 1283
Hpy8I GTNNAC 3 cut(s) 725, 875, 1319
Hpy99I CGWCG 4 cut(s) 44, 798, 1346, 1349
HpyAV CCTTC 8 cut(s) 609, 636, 663, 690, 744, 771, 1378, 1410
HpyCH4III ACNGT 2 cut(s) 969, 1146
HpyCH4IV ACGT 1 cut(s) 1210
HpyCH4V TGCA 5 cut(s) 494, 835, 1066, 1093, 1192
HpyF10VI GCNNNNNNNGC 4 cut(s) 943, 1090, 1189, 1409
HpyF3I CTNAG 4 cut(s) 273, 972, 993, 1167
HpySE526I ACGT 1 cut(s) 1210
Hsp92II CATG 4 cut(s) 677, 704, 758, 1033
HspAI GCGC 1 cut(s) 1403
KroI GCCGGC 1 cut(s) 942
KroNI GCCGGC 1 cut(s) 944
Ksp22I TGATCA 1 cut(s) 403
Kzo9I GATC 8 cut(s) 100, 239, 368, 383, 403, 450, 517, 1299
LguI GCTCTTC 2 cut(s) 353, 1422
LmnI GCTCC 1 cut(s) 225
Lsp1109I GCAGC 1 cut(s) 1096
LweI GCATC 4 cut(s) 481, 822, 966, 1237
MaeI CTAG 4 cut(s) 564, 950, 1335, 1437
MaeII ACGT 1 cut(s) 1210
MaeIII GTNAC 3 cut(s) 1134, 1206, 1337
MalI GATC 8 cut(s) 102, 241, 370, 385, 405, 452, 519, 1301
MboI GATC 8 cut(s) 100, 239, 368, 383, 403, 450, 517, 1299
MflI RGATCY 2 cut(s) 100, 239
MhlI GDGCHC 4 cut(s) 365, 501, 852, 1044
MlsI TGGCCA 1 cut(s) 194
MluCI AATT 3 cut(s) 161, 232, 547
MluNI TGGCCA 1 cut(s) 194
MlyI GAGTC 1 cut(s) 893
Mox20I TGGCCA 1 cut(s) 194
Mph1103I ATGCAT 1 cut(s) 837
MroNI GCCGGC 1 cut(s) 942
MscI TGGCCA 1 cut(s) 194
MseI TTAA 2 cut(s) 423, 432
MslI CAYNNNNRTG 1 cut(s) 1263
Msp20I TGGCCA 1 cut(s) 194
MspI CCGG 2 cut(s) 943, 1277
MspR9I CCNGG 4 cut(s) 789, 939, 1014, 1278
Mva1269I GAATGC 1 cut(s) 835
MvaI CCWGG 3 cut(s) 789, 939, 1014
MwoI GCNNNNNNNGC 4 cut(s) 943, 1090, 1189, 1409
NaeI GCCGGC 1 cut(s) 944
NciI CCSGG 1 cut(s) 1278
NdeII GATC 8 cut(s) 100, 239, 368, 383, 403, 450, 517, 1299
NgoMIV GCCGGC 1 cut(s) 942
NlaIII CATG 4 cut(s) 677, 704, 758, 1033
NlaIV GGNNCC 3 cut(s) 849, 850, 1259
NmuCI GTSAC 2 cut(s) 1206, 1337
NsiI ATGCAT 1 cut(s) 837
NspI RCATGY 3 cut(s) 677, 704, 758
PaeR7I CTCGAG 1 cut(s) 901
PceI AGGCCT 1 cut(s) 1017
PciI ACATGT 3 cut(s) 673, 700, 754
PciSI GCTCTTC 2 cut(s) 353, 1422
PcsI WCGNNNNNNNCGW 1 cut(s) 1338
PctI GAATGC 1 cut(s) 835
PdiI GCCGGC 1 cut(s) 944
PfeI GAWTC 4 cut(s) 283, 884, 1024, 1115
PflMI CCANNNNNTGG 1 cut(s) 551
PkrI GCNGC 1 cut(s) 1086
PleI GAGTC 1 cut(s) 893
PpsI GAGTC 1 cut(s) 893
PscI ACATGT 3 cut(s) 673, 700, 754
PshBI ATTAAT 1 cut(s) 423
Psp124BI GAGCTC 1 cut(s) 365
Psp6I CCWGG 3 cut(s) 787, 937, 1012
PspFI CCCAGC 1 cut(s) 502
PspGI CCWGG 3 cut(s) 787, 937, 1012
PspN4I GGNNCC 3 cut(s) 849, 850, 1259
PspOMI GGGCCC 1 cut(s) 848
PspPI GGNCC 4 cut(s) 251, 848, 849, 935
PsuI RGATCY 2 cut(s) 100, 239
RigI GGCCGGCC 1 cut(s) 946
RsaI GTAC 7 cut(s) 444, 651, 678, 705, 759, 786, 1110
RsaNI GTAC 7 cut(s) 443, 650, 677, 704, 758, 785, 1109
RseI CAYNNNNRTG 1 cut(s) 1263
SacI GAGCTC 1 cut(s) 365
SapI GCTCTTC 2 cut(s) 353, 1422
SaqAI TTAA 2 cut(s) 423, 432
SatI GCNGC 1 cut(s) 1085
Sau3AI GATC 8 cut(s) 100, 239, 368, 383, 403, 450, 517, 1299
Sau96I GGNCC 4 cut(s) 251, 848, 849, 935
SchI GAGTC 1 cut(s) 893
ScrFI CCNGG 4 cut(s) 789, 939, 1014, 1278
SduI GDGCHC 4 cut(s) 365, 501, 852, 1044
SfaNI GCATC 4 cut(s) 481, 822, 966, 1237
SfiI GGCCNNNNNGGCC 1 cut(s) 943
Sfr274I CTCGAG 1 cut(s) 901
SlaI CTCGAG 1 cut(s) 901
SmiMI CAYNNNNRTG 1 cut(s) 1263
SmlI CTYRAG 3 cut(s) 134, 901, 1221
SmoI CTYRAG 3 cut(s) 134, 901, 1221
Sse9I AATT 3 cut(s) 161, 232, 547
SseBI AGGCCT 1 cut(s) 1017
SsiI CCGC 2 cut(s) 4, 559
SspMI CTAG 4 cut(s) 564, 950, 1335, 1437
SstI GAGCTC 1 cut(s) 365
StuI AGGCCT 1 cut(s) 1017
StyD4I CCNGG 4 cut(s) 787, 937, 1012, 1276
StyI CCWWGG 2 cut(s) 114, 1079
TaaI ACNGT 2 cut(s) 969, 1146
TaiI ACGT 1 cut(s) 1213
TaqI TCGA 3 cut(s) 386, 520, 902
TaqII GACCGA 1 cut(s) 1490
TasI AATT 3 cut(s) 161, 232, 547
TatI WGTACW 2 cut(s) 442, 703
TfiI GAWTC 4 cut(s) 283, 884, 1024, 1115
Tru1I TTAA 2 cut(s) 423, 432
Tru9I TTAA 2 cut(s) 423, 432
TscAI CASTG 2 cut(s) 334, 997
TseFI GTSAC 2 cut(s) 1206, 1337
TseI GCWGC 1 cut(s) 1084
Tsp45I GTSAC 2 cut(s) 1206, 1337
TspDTI ATGAA 7 cut(s) 99, 591, 618, 816, 836, 1287, 1397
TspGWI ACGGA 1 cut(s) 1483
TspRI CASTG 2 cut(s) 334, 997
Van91I CCANNNNNTGG 1 cut(s) 551
VspI ATTAAT 1 cut(s) 423
XapI RAATTY 1 cut(s) 547
XbaI TCTAGA 1 cut(s) 949
XceI RCATGY 3 cut(s) 677, 704, 758
XcmI CCANNNNNNNNNTGG 1 cut(s) 121
XhoI CTCGAG 1 cut(s) 901
XmiI GTMKAC 2 cut(s) 724, 874
XspI CTAG 4 cut(s) 564, 950, 1335, 1437
Zsp2I ATGCAT 1 cut(s) 837
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.