Rroxscaffold_2G00134030

SPT2 chromatin protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0003861
Physical Location & Seq
Forward (+)
71513261 .. 71516411
3151 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00134030.1

Sequence Viewer

Length: 1506 bp
ATGCGGGGATATGATACACAGAGATTCGATCGACCAGTAGAGGAATATGATGGTTATGAGTCCGAGGGGGATGGATATGGATACGAGGAGGAAGGAGATGAGTATGAAGATGAAGAGGAAGAGGAAGAAGAAGCTCCAAAACCTACCAAGGAAGCATTGGAGTACCTTGAGTTGAGACAACGCTTGAAAGAACAAGTTCGAAGGCAGATGAAGAAGAAGGAAGGTGGTTCTCGGGCCAACTCCAATGATAGAAAGAAGCTCCCCTATGATAATTTTGGATCCTTCTTTGGCCCTTCTCAACCAGTTATCGCTCAGAGAGTAATTCAAGAAAGCAAGTCGTTATTGGAAACCCGGCATCTGGCATCTAGGGCCACAAACTCTGTCCATCCTAACAAGAAGAGCTCTGGGTCTACCTCTGGTGGCTCAAAATCTGTTGCACATAACCCGAAACCCAAAGTAATTAATGAGCAAAAAAATAAACTCCAAAAGCTTAAAGACACTAGAGATTACTCATTTTTATTATCTGAAGATGCGGAGCTTCCTGCTCCTGCAAAGGATCGTCCCCCTCGAAGTGACTCTGCTCTGAAGTCTGAGGTGCGATCAAGTCAAACAATGGCAAAAAGTAAACAACCTTTGGCAAATAATGGTAGACCTGTCCATGGTGGTCATGATAATGGTAGATCTGTTCATGGCAGTCGTGAGAATGGGAGACCTGTTCAGGGCAGTCGTGAGAATGGGAGACCTGTTCAAGGCAGTCGTGAGAATGGGAGACCTGTTCAGTCTGGTCGTGATGAAAGGAAAGTGGTTCCTACTAATGGACATATGCATTCTAAAGTTGGGTCCAATAGGCCAAGTTCTGCCAGTAGCCGGCCTGGTTCAACATCAATGGACTCTAGAAAACAACTTGGTAGCAACAGTGCAAATGGGCCTGGCCGGCCTCTAGGGCCAAAAGGTCTGCCTTCAAAGATGCCTGCTTCTACCACAGAGAGGAGGGCTTCTGCACCAGGTTTGAAGAATTCCCAGTCTTCTTTGCAGAAAGCACCCTCTTCAAAGTCGCAGTCATCTGTTCAGAGGCAGCCAGTACAGCTAAGAAAAGAAGTACGAGAACCTTATAAGCCCAATATGTTATCAAAACAGTCCACGGGATTGTCAAAACCTCAGATCCACAAGCCACAAATGCATAAGCAAATCCCATCACGTCCTATGTCACAAGATCGTCCCAAAAAAAGGCCTGCAAGCAGGTTTGCAGATGATGAGTATGACCCTGAATCGGGGGATATTAGTAGTATGATCAGAAACATGTTTGGTTACAATCCAAATAAGTTTGCTGATGACGATGACGTTAGTGACATGGAGGCAGGATTTGAAGATATTCAGAGGGAGGAGAGGAGAAGTGCAAAAATTGCAAGACAGGAGGATGAAGAACAAGCTAGGTTGATAGAAGAAGAAGAAAGGAGGGAACGTTTGGCCGCCAAGATGAGAAAACAGAAGAAGCTTAAGCGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
SPT2

Protein Analysis

501

Amino Acids

56.31

Weight (kDa)

9.66

Isoelectric Point (pI)

72.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SPT2 PF08243 400 - 488 1.7e-18 SPT2 chromatin protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1113
Acc36I ACCTGC 1 cut(s) 1230
AccI GTMKAC 2 cut(s) 410, 649
AciI CCGC 3 cut(s) 4, 533, 1470
AclI AACGTT 1 cut(s) 1462
AclWI GGATC 4 cut(s) 273, 286, 564, 1156
AcoI YGGCCR 2 cut(s) 931, 1467
AcsI RAATTY 1 cut(s) 1015
AcuI CTGAAG 2 cut(s) 546, 605
AfaI GTAC 3 cut(s) 164, 1083, 1101
AflII CTTAAG 1 cut(s) 1496
AflIII ACRYGT 1 cut(s) 1299
AgsI TTSAA 8 cut(s) 187, 326, 749, 879, 963, 1012, 1050, 1367
AjiI CACGTC 1 cut(s) 1199
AjnI CCWGG 3 cut(s) 871, 928, 1003
AluBI AGCT 8 cut(s) 134, 259, 402, 490, 538, 1087, 1430, 1495
AluI AGCT 8 cut(s) 134, 259, 402, 490, 538, 1087, 1430, 1495
Alw21I GWGCWC 1 cut(s) 404
Alw26I GTCTC 4 cut(s) 169, 703, 733, 763
AlwI GGATC 4 cut(s) 273, 286, 564, 1156
Ama87I CYCGRG 1 cut(s) 231
ApeKI GCWGC 1 cut(s) 1075
ApoI RAATTY 1 cut(s) 1015
ArsI GACNNNNNNTTYG 2 cut(s) 1043, 1075
AseI ATTAAT 1 cut(s) 462
Asp700I GAANNNNTTC 2 cut(s) 195, 1371
AspS9I GGNCC 6 cut(s) 234, 290, 369, 840, 926, 944
AsuC2I CCSGG 1 cut(s) 352
AsuII TTCGAA 1 cut(s) 199
AvaI CYCGRG 1 cut(s) 231
AvaII GGWCC 1 cut(s) 840
BamHI GGATCC 1 cut(s) 278
BanII GRGCYC 1 cut(s) 404
BbsI GAAGAC 1 cut(s) 1017
Bbv12I GWGCWC 1 cut(s) 404
BbvI GCAGC 1 cut(s) 1087
BccI CCATC 4 cut(s) 44, 65, 393, 1201
BciT130I CCWGG 3 cut(s) 873, 930, 1005
BciVI GTATCC 1 cut(s) 74
BclI TGATCA 1 cut(s) 1290
BcnI CCSGG 1 cut(s) 352
BcoDI GTCTC 4 cut(s) 169, 703, 733, 763
BfaI CTAG 5 cut(s) 366, 501, 894, 941, 1431
BfrI CTTAAG 1 cut(s) 1496
BfuAI ACCTGC 1 cut(s) 1230
BfuI GTATCC 1 cut(s) 74
BglI GCCNNNNNGGC 2 cut(s) 934, 943
BglII AGATCT 1 cut(s) 680
BisI GCNGC 2 cut(s) 1076, 1470
BlsI GCNGC 2 cut(s) 1077, 1471
Bme1390I CCNGG 4 cut(s) 352, 873, 930, 1005
Bme18I GGWCC 1 cut(s) 840
BmeT110I CYCGRG 1 cut(s) 231
BmgBI CACGTC 1 cut(s) 1199
BmgT120I GGNCC 6 cut(s) 234, 290, 369, 840, 926, 944
BmiI GGNNCC 3 cut(s) 280, 807, 841
BmrFI CCNGG 4 cut(s) 352, 873, 930, 1005
BmrI ACTGGG 1 cut(s) 1015
BmsI GCATC 4 cut(s) 364, 371, 520, 957
BmuI ACTGGG 1 cut(s) 1015
BpiI GAAGAC 1 cut(s) 1017
Bpu14I TTCGAA 1 cut(s) 199
BpuEI CTTGAG 1 cut(s) 188
BpuMI CCSGG 1 cut(s) 352
BsaI GGTCTC 3 cut(s) 703, 733, 763
BsaJI CCNNGG 4 cut(s) 63, 147, 658, 1140
BsaXI ACNNNNNCTCC 2 cut(s) 1447, 1477
Bse118I RCCGGY 2 cut(s) 867, 933
Bse1I ACTGG 5 cut(s) 35, 302, 861, 1021, 1079
BseBI CCWGG 3 cut(s) 873, 930, 1005
BseDI CCNNGG 4 cut(s) 63, 147, 658, 1140
BseGI GGATG 3 cut(s) 76, 385, 1423
BseMII CTCAG 3 cut(s) 326, 582, 1172
BseNI ACTGG 5 cut(s) 35, 302, 861, 1021, 1079
BseRI GAGGAG 4 cut(s) 101, 1003, 1397, 1402
BseXI GCAGC 1 cut(s) 1087
BsgI GTGCAG 1 cut(s) 984
Bsh1285I CGRYCG 1 cut(s) 31
BsiEI CGRYCG 1 cut(s) 31
BsiHKAI GWGCWC 1 cut(s) 404
BsiHKCI CYCGRG 1 cut(s) 231
BsiSI CCGG 3 cut(s) 352, 868, 934
BslFI GGGAC 2 cut(s) 546, 1203
BsmAI GTCTC 4 cut(s) 169, 703, 733, 763
BsmFI GGGAC 2 cut(s) 546, 1203
BsmI GAATGC 1 cut(s) 826
Bso31I GGTCTC 3 cut(s) 703, 733, 763
BsoBI CYCGRG 1 cut(s) 231
Bsp119I TTCGAA 1 cut(s) 199
Bsp1286I GDGCHC 1 cut(s) 404
Bsp143I GATC 8 cut(s) 28, 278, 556, 599, 680, 1161, 1213, 1290
Bsp19I CCATGG 1 cut(s) 658
BspACI CCGC 3 cut(s) 4, 533, 1470
BspCNI CTCAG 3 cut(s) 325, 583, 1171
BspHI TCATGA 1 cut(s) 667
BspLI GGNNCC 3 cut(s) 280, 807, 841
BspMI ACCTGC 1 cut(s) 1230
BspPI GGATC 4 cut(s) 273, 286, 564, 1156
BspQI GCTCTTC 1 cut(s) 392
BspT104I TTCGAA 1 cut(s) 199
BspTI CTTAAG 1 cut(s) 1496
BspTNI GGTCTC 3 cut(s) 703, 733, 763
BsrFI RCCGGY 2 cut(s) 867, 933
BsrI ACTGG 5 cut(s) 35, 302, 861, 1021, 1079
BssAI RCCGGY 2 cut(s) 867, 933
BssECI CCNNGG 4 cut(s) 63, 147, 658, 1140
BssMI GATC 8 cut(s) 28, 278, 556, 599, 680, 1161, 1213, 1290
BssT1I CCWWGG 2 cut(s) 147, 658
Bst2UI CCWGG 3 cut(s) 873, 930, 1005
Bst4CI ACNGT 2 cut(s) 917, 1137
Bst6I CTCTTC 4 cut(s) 108, 114, 392, 1051
BstAFI CTTAAG 1 cut(s) 1496
BstAPI GCANNNNNTGC 1 cut(s) 1403
BstBI TTCGAA 1 cut(s) 199
BstC8I GCNNGC 5 cut(s) 869, 935, 972, 1233, 1237
BstDEI CTNAG 4 cut(s) 312, 591, 1088, 1158
BstDSI CCRYGG 2 cut(s) 658, 1140
BstENI CCTNNNNNAGG 1 cut(s) 747
BstF5I GGATG 3 cut(s) 76, 385, 1423
BstKTI GATC 8 cut(s) 31, 281, 559, 602, 683, 1164, 1216, 1293
BstMAI GTCTC 4 cut(s) 169, 703, 733, 763
BstMBI GATC 8 cut(s) 28, 278, 556, 599, 680, 1161, 1213, 1290
BstMCI CGRYCG 1 cut(s) 31
BstMWI GCNNNNNNNGC 6 cut(s) 368, 934, 943, 1084, 1177, 1403
BstNI CCWGG 3 cut(s) 873, 930, 1005
BstNSI RCATGY 1 cut(s) 1303
BstSCI CCNGG 4 cut(s) 350, 871, 928, 1003
BstV1I GCAGC 1 cut(s) 1087
BstV2I GAAGAC 1 cut(s) 1017
BstX2I RGATCY 3 cut(s) 278, 680, 1161
BstYI RGATCY 3 cut(s) 278, 680, 1161
BsuI GTATCC 1 cut(s) 74
BtgI CCRYGG 2 cut(s) 658, 1140
BtrI CACGTC 1 cut(s) 1199
BtsCI GGATG 3 cut(s) 76, 385, 1423
BtsIMutI CAGTG 1 cut(s) 922
BveI ACCTGC 1 cut(s) 1230
Cac8I GCNNGC 5 cut(s) 869, 935, 972, 1233, 1237
CciI TCATGA 1 cut(s) 667
Cfr10I RCCGGY 2 cut(s) 867, 933
Cfr13I GGNCC 6 cut(s) 234, 290, 369, 840, 926, 944
CsiI ACCWGGT 1 cut(s) 1003
Csp6I GTAC 3 cut(s) 163, 1082, 1100
CviAII CATG 5 cut(s) 659, 668, 689, 1300, 1351
CviQI GTAC 3 cut(s) 163, 1082, 1100
DdeI CTNAG 4 cut(s) 312, 591, 1088, 1158
DpnI GATC 8 cut(s) 30, 280, 558, 601, 682, 1163, 1215, 1292
DpnII GATC 8 cut(s) 28, 278, 556, 599, 680, 1161, 1213, 1290
EaeI YGGCCR 2 cut(s) 931, 1467
Eam1104I CTCTTC 4 cut(s) 108, 114, 392, 1051
EarI CTCTTC 4 cut(s) 108, 114, 392, 1051
Ecl136II GAGCTC 1 cut(s) 402
Eco130I CCWWGG 2 cut(s) 147, 658
Eco147I AGGCCT 1 cut(s) 1231
Eco24I GRGCYC 1 cut(s) 404
Eco31I GGTCTC 3 cut(s) 703, 733, 763
Eco47I GGWCC 1 cut(s) 840
Eco53kI GAGCTC 1 cut(s) 402
Eco57I CTGAAG 2 cut(s) 546, 605
Eco88I CYCGRG 1 cut(s) 231
EcoICRI GAGCTC 1 cut(s) 402
EcoNI CCTNNNNNAGG 1 cut(s) 747
EcoRI GAATTC 1 cut(s) 1015
EcoRII CCWGG 3 cut(s) 871, 928, 1003
EcoT14I CCWWGG 2 cut(s) 147, 658
EcoT22I ATGCAT 2 cut(s) 828, 1182
EcoT38I GRGCYC 1 cut(s) 404
ErhI CCWWGG 2 cut(s) 147, 658
FaeI CATG 5 cut(s) 662, 671, 692, 1303, 1354
FaqI GGGAC 2 cut(s) 546, 1203
FatI CATG 5 cut(s) 658, 667, 688, 1299, 1350
FauNDI CATATG 1 cut(s) 822
FbaI TGATCA 1 cut(s) 1290
FblI GTMKAC 2 cut(s) 410, 649
Fnu4HI GCNGC 2 cut(s) 1076, 1470
FokI GGATG 3 cut(s) 83, 372, 1430
FriOI GRGCYC 1 cut(s) 404
FseI GGCCGGCC 1 cut(s) 937
Fsp4HI GCNGC 2 cut(s) 1076, 1470
FspBI CTAG 5 cut(s) 366, 501, 894, 941, 1431
GluI GCNGC 2 cut(s) 1076, 1470
HapII CCGG 3 cut(s) 352, 868, 934
Hin1II CATG 5 cut(s) 662, 671, 692, 1303, 1354
HindIII AAGCTT 2 cut(s) 488, 1493
HinfI GANTC 5 cut(s) 24, 59, 575, 890, 1268
HpaII CCGG 3 cut(s) 352, 868, 934
Hpy166II GTNNAC 4 cut(s) 411, 626, 650, 1140
Hpy188I TCNGA 9 cut(s) 64, 315, 526, 585, 592, 1071, 1161, 1295, 1377
Hpy188III TCNNGA 7 cut(s) 326, 668, 698, 728, 758, 788, 894
Hpy8I GTNNAC 4 cut(s) 411, 626, 650, 1140
HpyAV CCTTC 7 cut(s) 86, 195, 211, 215, 292, 303, 969
HpyCH4III ACNGT 2 cut(s) 917, 1137
HpyCH4IV ACGT 3 cut(s) 1198, 1341, 1462
HpyF10VI GCNNNNNNNGC 6 cut(s) 368, 934, 943, 1084, 1177, 1403
HpyF3I CTNAG 4 cut(s) 312, 591, 1088, 1158
HpySE526I ACGT 3 cut(s) 1198, 1341, 1462
Hsp92II CATG 5 cut(s) 662, 671, 692, 1303, 1354
KroI GCCGGC 2 cut(s) 867, 933
KroNI GCCGGC 2 cut(s) 869, 935
Ksp22I TGATCA 1 cut(s) 1290
Kzo9I GATC 8 cut(s) 28, 278, 556, 599, 680, 1161, 1213, 1290
LguI GCTCTTC 1 cut(s) 392
LmnI GCTCC 4 cut(s) 139, 264, 535, 550
Lsp1109I GCAGC 1 cut(s) 1087
LweI GCATC 4 cut(s) 364, 371, 520, 957
MabI ACCWGGT 1 cut(s) 1003
MaeI CTAG 5 cut(s) 366, 501, 894, 941, 1431
MaeII ACGT 3 cut(s) 1198, 1341, 1462
MaeIII GTNAC 4 cut(s) 572, 1206, 1307, 1346
MalI GATC 8 cut(s) 30, 280, 558, 601, 682, 1163, 1215, 1292
MboI GATC 8 cut(s) 28, 278, 556, 599, 680, 1161, 1213, 1290
MflI RGATCY 3 cut(s) 278, 680, 1161
MhlI GDGCHC 1 cut(s) 404
MluCI AATT 5 cut(s) 271, 321, 459, 1015, 1401
MlyI GAGTC 3 cut(s) 68, 569, 884
Mph1103I ATGCAT 2 cut(s) 828, 1182
MroNI GCCGGC 2 cut(s) 867, 933
MroXI GAANNNNTTC 2 cut(s) 195, 1371
MseI TTAA 3 cut(s) 462, 492, 1497
MslI CAYNNNNRTG 1 cut(s) 672
MspCI CTTAAG 1 cut(s) 1496
MspI CCGG 3 cut(s) 352, 868, 934
MspR9I CCNGG 4 cut(s) 352, 873, 930, 1005
Mva1269I GAATGC 1 cut(s) 826
MvaI CCWGG 3 cut(s) 873, 930, 1005
MwoI GCNNNNNNNGC 6 cut(s) 368, 934, 943, 1084, 1177, 1403
NaeI GCCGGC 2 cut(s) 869, 935
NciI CCSGG 1 cut(s) 352
NcoI CCATGG 1 cut(s) 658
NdeI CATATG 1 cut(s) 822
NdeII GATC 8 cut(s) 28, 278, 556, 599, 680, 1161, 1213, 1290
NgoMIV GCCGGC 2 cut(s) 867, 933
NlaIII CATG 5 cut(s) 662, 671, 692, 1303, 1354
NlaIV GGNNCC 3 cut(s) 280, 807, 841
NmuCI GTSAC 3 cut(s) 572, 1206, 1346
NsiI ATGCAT 2 cut(s) 828, 1182
NspI RCATGY 1 cut(s) 1303
NspV TTCGAA 1 cut(s) 199
PagI TCATGA 1 cut(s) 667
PceI AGGCCT 1 cut(s) 1231
PciI ACATGT 1 cut(s) 1299
PciSI GCTCTTC 1 cut(s) 392
PcsI WCGNNNNNNNCGW 1 cut(s) 565
PctI GAATGC 1 cut(s) 826
PdiI GCCGGC 2 cut(s) 869, 935
PdmI GAANNNNTTC 2 cut(s) 195, 1371
PfeI GAWTC 2 cut(s) 24, 1268
PkrI GCNGC 2 cut(s) 1077, 1471
Ple19I CGATCG 1 cut(s) 31
PleI GAGTC 3 cut(s) 67, 569, 884
PpsI GAGTC 3 cut(s) 67, 569, 884
PscI ACATGT 1 cut(s) 1299
PshBI ATTAAT 1 cut(s) 462
PsiI TTATAA 1 cut(s) 1113
Psp124BI GAGCTC 1 cut(s) 404
Psp1406I AACGTT 1 cut(s) 1462
Psp6I CCWGG 3 cut(s) 871, 928, 1003
PspGI CCWGG 3 cut(s) 871, 928, 1003
PspN4I GGNNCC 3 cut(s) 280, 807, 841
PspPI GGNCC 6 cut(s) 234, 290, 369, 840, 926, 944
PsuI RGATCY 3 cut(s) 278, 680, 1161
PvuI CGATCG 1 cut(s) 31
RigI GGCCGGCC 1 cut(s) 937
RsaI GTAC 3 cut(s) 164, 1083, 1101
RsaNI GTAC 3 cut(s) 163, 1082, 1100
RseI CAYNNNNRTG 1 cut(s) 672
SacI GAGCTC 1 cut(s) 404
SapI GCTCTTC 1 cut(s) 392
SaqAI TTAA 3 cut(s) 462, 492, 1497
SatI GCNGC 2 cut(s) 1076, 1470
Sau3AI GATC 8 cut(s) 28, 278, 556, 599, 680, 1161, 1213, 1290
Sau96I GGNCC 6 cut(s) 234, 290, 369, 840, 926, 944
SchI GAGTC 3 cut(s) 68, 569, 884
ScrFI CCNGG 4 cut(s) 352, 873, 930, 1005
SduI GDGCHC 1 cut(s) 404
SexAI ACCWGGT 1 cut(s) 1003
SfaNI GCATC 4 cut(s) 364, 371, 520, 957
SfiI GGCCNNNNNGGCC 2 cut(s) 934, 943
SfuI TTCGAA 1 cut(s) 199
SinI GGWCC 1 cut(s) 840
SmiMI CAYNNNNRTG 1 cut(s) 672
SmlI CTYRAG 2 cut(s) 167, 1496
SmoI CTYRAG 2 cut(s) 167, 1496
Sse9I AATT 5 cut(s) 271, 321, 459, 1015, 1401
SseBI AGGCCT 1 cut(s) 1231
SsiI CCGC 3 cut(s) 4, 533, 1470
SspMI CTAG 5 cut(s) 366, 501, 894, 941, 1431
SstI GAGCTC 1 cut(s) 404
StuI AGGCCT 1 cut(s) 1231
StyD4I CCNGG 4 cut(s) 350, 871, 928, 1003
StyI CCWWGG 2 cut(s) 147, 658
TaaI ACNGT 2 cut(s) 917, 1137
TaiI ACGT 3 cut(s) 1201, 1344, 1465
TaqI TCGA 4 cut(s) 27, 31, 199, 568
TasI AATT 5 cut(s) 271, 321, 459, 1015, 1401
TatI WGTACW 1 cut(s) 1081
TauI GCSGC 1 cut(s) 1472
TfiI GAWTC 2 cut(s) 24, 1268
Tru1I TTAA 3 cut(s) 462, 492, 1497
Tru9I TTAA 3 cut(s) 462, 492, 1497
TscAI CASTG 1 cut(s) 922
TseFI GTSAC 3 cut(s) 572, 1206, 1346
TseI GCWGC 1 cut(s) 1075
Tsp45I GTSAC 3 cut(s) 572, 1206, 1346
TspDTI ATGAA 6 cut(s) 120, 126, 224, 677, 807, 1434
TspRI CASTG 1 cut(s) 922
Vha464I CTTAAG 1 cut(s) 1496
VpaK11BI GGWCC 1 cut(s) 840
VspI ATTAAT 1 cut(s) 462
XagI CCTNNNNNAGG 1 cut(s) 747
XapI RAATTY 1 cut(s) 1015
XbaI TCTAGA 1 cut(s) 893
XceI RCATGY 1 cut(s) 1303
XcmI CCANNNNNNNNNTGG 1 cut(s) 154
XmiI GTMKAC 2 cut(s) 410, 649
XmnI GAANNNNTTC 2 cut(s) 195, 1371
XspI CTAG 5 cut(s) 366, 501, 894, 941, 1431
Zsp2I ATGCAT 2 cut(s) 828, 1182
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.