MD02G1314900.v1.1

U6 snRNA-associated Sm-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0008526
Physical Location & Seq
Reverse (-)
36989727 .. 36991469
1743 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1314900.v1.1.491

Sequence Viewer

Length: 309 bp
ATGTCAGGGAGGAAAGAAACAGTTTTGGACCTGGCCAAGTTTGTGGACAAGGGTGTCCAAGTCAAGCTCACCGGCGGTAGACAAGTGACAGGGACTCTTAAAGGTTATGACCAGTTGTTAAACCTTGTTCTAGATGAAGCAGTGGAGTTTCTAAGAGATTCTGATGATCCGTTGAAGACCACTGATCAGACCAGGCGCCTTGGCCTCATAGTATGTATTCTTCTAGTTTATCTCTGTTGTAGGATCTTAAGAAAACTGCACGGGAACTTGATATATATTGATGTTGGCTGTTGTAGGATCTATGTTTAG

Protein Analysis

103

Amino Acids

11.5

Weight (kDa)

8.65

Isoelectric Point (pI)

17.21

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LSM PF01423 10 - 71 6.3e-16 LSM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 53
AccB1I GGYRCC 1 cut(s) 195
AccI GTMKAC 1 cut(s) 79
AciI CCGC 1 cut(s) 75
AclWI GGATC 3 cut(s) 161, 251, 305
AcoI YGGCCR 1 cut(s) 33
AcyI GRCGYC 1 cut(s) 196
AflII CTTAAG 1 cut(s) 247
AgsI TTSAA 1 cut(s) 175
AjnI CCWGG 2 cut(s) 30, 191
AluBI AGCT 1 cut(s) 67
AluI AGCT 1 cut(s) 67
AlwI GGATC 3 cut(s) 161, 251, 305
AoxI GGCC 2 cut(s) 33, 202
AspLEI GCGC 1 cut(s) 198
AspS9I GGNCC 1 cut(s) 28
AsuHPI GGTGA 1 cut(s) 61
AvaII GGWCC 1 cut(s) 28
BalI TGGCCA 1 cut(s) 35
BanI GGYRCC 1 cut(s) 195
BarI GAAGNNNNNNTAC 2 cut(s) 204, 236
BbsI GAAGAC 1 cut(s) 182
BciT130I CCWGG 2 cut(s) 32, 193
BclI TGATCA 1 cut(s) 184
BfaI CTAG 2 cut(s) 131, 224
BfoI RGCGCY 1 cut(s) 199
BfrI CTTAAG 1 cut(s) 247
Bme1390I CCNGG 2 cut(s) 32, 193
Bme18I GGWCC 1 cut(s) 28
BmgT120I GGNCC 1 cut(s) 28
BmiI GGNNCC 1 cut(s) 197
BmrFI CCNGG 2 cut(s) 32, 193
BpiI GAAGAC 1 cut(s) 182
BsaHI GRCGYC 1 cut(s) 196
BsaJI CCNNGG 1 cut(s) 199
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 1 cut(s) 112
BseBI CCWGG 2 cut(s) 32, 193
BseDI CCNNGG 1 cut(s) 199
BseNI ACTGG 1 cut(s) 112
BsgI GTGCAG 1 cut(s) 242
BshFI GGCC 2 cut(s) 35, 204
BshNI GGYRCC 1 cut(s) 195
BsiSI CCGG 1 cut(s) 72
BslFI GGGAC 1 cut(s) 106
BsmFI GGGAC 1 cut(s) 106
BsnI GGCC 2 cut(s) 35, 204
Bsp143I GATC 4 cut(s) 166, 184, 243, 297
BspACI CCGC 1 cut(s) 75
BspANI GGCC 2 cut(s) 35, 204
BspLI GGNNCC 1 cut(s) 197
BspPI GGATC 3 cut(s) 161, 251, 305
BspT107I GGYRCC 1 cut(s) 195
BspTI CTTAAG 1 cut(s) 247
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 1 cut(s) 112
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 1 cut(s) 199
BssMI GATC 4 cut(s) 166, 184, 243, 297
BssNI GRCGYC 1 cut(s) 196
BssT1I CCWWGG 1 cut(s) 199
Bst2UI CCWGG 2 cut(s) 32, 193
Bst4CI ACNGT 1 cut(s) 22
BstACI GRCGYC 1 cut(s) 196
BstAFI CTTAAG 1 cut(s) 247
BstDEI CTNAG 1 cut(s) 152
BstH2I RGCGCY 1 cut(s) 199
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 4 cut(s) 169, 187, 246, 300
BstMBI GATC 4 cut(s) 166, 184, 243, 297
BstNI CCWGG 2 cut(s) 32, 193
BstSCI CCNGG 2 cut(s) 30, 191
BstV2I GAAGAC 1 cut(s) 182
BstX2I RGATCY 2 cut(s) 243, 297
BstXI CCANNNNNNTGG 1 cut(s) 43
BstYI RGATCY 2 cut(s) 243, 297
BsuRI GGCC 2 cut(s) 35, 204
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 2 cut(s) 147, 180
CfoI GCGC 1 cut(s) 198
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 1 cut(s) 28
CviJI RGCY 4 cut(s) 35, 67, 204, 288
CviKI_1 RGCY 4 cut(s) 35, 67, 204, 288
DdeI CTNAG 1 cut(s) 152
DinI GGCGCC 1 cut(s) 197
DpnI GATC 4 cut(s) 168, 186, 245, 299
DpnII GATC 4 cut(s) 166, 184, 243, 297
DrdI GACNNNNNNGTC 1 cut(s) 53
DseDI GACNNNNNNGTC 1 cut(s) 53
EaeI YGGCCR 1 cut(s) 33
Eco130I CCWWGG 1 cut(s) 199
Eco47I GGWCC 1 cut(s) 28
EcoRII CCWGG 2 cut(s) 30, 191
EcoT14I CCWWGG 1 cut(s) 199
EgeI GGCGCC 1 cut(s) 197
EheI GGCGCC 1 cut(s) 197
ErhI CCWWGG 1 cut(s) 199
FaiI YATR 6 cut(s) 108, 209, 214, 274, 276, 303
FaqI GGGAC 1 cut(s) 106
FbaI TGATCA 1 cut(s) 184
FblI GTMKAC 1 cut(s) 79
FspBI CTAG 2 cut(s) 131, 224
GlaI GCGC 1 cut(s) 197
HaeII RGCGCY 1 cut(s) 199
HaeIII GGCC 2 cut(s) 35, 204
HapII CCGG 1 cut(s) 72
HhaI GCGC 1 cut(s) 198
Hin1I GRCGYC 1 cut(s) 196
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HinfI GANTC 2 cut(s) 94, 158
HpaII CCGG 1 cut(s) 72
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 2 cut(s) 46, 80
Hpy188I TCNGA 2 cut(s) 163, 189
Hpy188III TCNNGA 1 cut(s) 131
Hpy8I GTNNAC 2 cut(s) 46, 80
HpyCH4III ACNGT 1 cut(s) 22
HpyCH4V TGCA 1 cut(s) 259
HpyF3I CTNAG 1 cut(s) 152
Hsp92I GRCGYC 1 cut(s) 196
HspAI GCGC 1 cut(s) 196
KasI GGCGCC 1 cut(s) 195
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 4 cut(s) 166, 184, 243, 297
LpnPI CCDG 7 cut(s) 17, 44, 75, 85, 125, 178, 205
MaeI CTAG 2 cut(s) 131, 224
MaeIII GTNAC 1 cut(s) 85
MalI GATC 4 cut(s) 168, 186, 245, 299
MboI GATC 4 cut(s) 166, 184, 243, 297
MboII GAAGA 2 cut(s) 187, 212
MflI RGATCY 2 cut(s) 243, 297
MlsI TGGCCA 1 cut(s) 35
MluNI TGGCCA 1 cut(s) 35
Mly113I GGCGCC 1 cut(s) 196
MlyI GAGTC 1 cut(s) 88
MnlI CCTC 2 cut(s) 3, 215
Mox20I TGGCCA 1 cut(s) 35
MscI TGGCCA 1 cut(s) 35
MseI TTAA 3 cut(s) 99, 119, 248
Msp20I TGGCCA 1 cut(s) 35
MspCI CTTAAG 1 cut(s) 247
MspI CCGG 1 cut(s) 72
MspR9I CCNGG 2 cut(s) 32, 193
MvaI CCWGG 2 cut(s) 32, 193
NarI GGCGCC 1 cut(s) 196
NdeII GATC 4 cut(s) 166, 184, 243, 297
NlaIV GGNNCC 1 cut(s) 197
NmuCI GTSAC 1 cut(s) 85
PfeI GAWTC 1 cut(s) 158
PleI GAGTC 1 cut(s) 88
PluTI GGCGCC 1 cut(s) 199
PpsI GAGTC 1 cut(s) 88
Psp6I CCWGG 2 cut(s) 30, 191
PspGI CCWGG 2 cut(s) 30, 191
PspN4I GGNNCC 1 cut(s) 197
PspPI GGNCC 1 cut(s) 28
PsuI RGATCY 2 cut(s) 243, 297
SaqAI TTAA 3 cut(s) 99, 119, 248
Sau3AI GATC 4 cut(s) 166, 184, 243, 297
Sau96I GGNCC 1 cut(s) 28
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 2 cut(s) 32, 193
SetI ASST 4 cut(s) 33, 69, 106, 126
SfoI GGCGCC 1 cut(s) 197
SgrAI CRCCGGYG 1 cut(s) 71
SinI GGWCC 1 cut(s) 28
SmlI CTYRAG 1 cut(s) 247
SmoI CTYRAG 1 cut(s) 247
SsiI CCGC 1 cut(s) 75
SspDI GGCGCC 1 cut(s) 195
SspMI CTAG 2 cut(s) 131, 224
StyD4I CCNGG 2 cut(s) 30, 191
StyI CCWWGG 1 cut(s) 199
TaaI ACNGT 1 cut(s) 22
TfiI GAWTC 1 cut(s) 158
Tru1I TTAA 3 cut(s) 99, 119, 248
Tru9I TTAA 3 cut(s) 99, 119, 248
TscAI CASTG 2 cut(s) 147, 187
TseFI GTSAC 1 cut(s) 85
Tsp45I GTSAC 1 cut(s) 85
TspDTI ATGAA 1 cut(s) 150
TspGWI ACGGA 1 cut(s) 159
TspRI CASTG 2 cut(s) 147, 187
Vha464I CTTAAG 1 cut(s) 247
VpaK11BI GGWCC 1 cut(s) 28
XbaI TCTAGA 1 cut(s) 130
XmiI GTMKAC 1 cut(s) 79
XspI CTAG 2 cut(s) 131, 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.