Basic Information

Type: gene

Biological Identity

Species

rosa_rugosa

Chromosome

GWHBQTZ00000001

Orthogroup ID

OG0008526

Physical Location & Seq

Genomic Coordinates Forward (+)

780355 .. 784641

Gene Structure 4287 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rorug01G0003700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3582 bp

ATGGCGTCAACCTCTTCTCGTCGTCAATGGAAATACGATGTGTTTCTAAGTTTCAGAGGGGCAGATACCCGCAGTGTTTTTACCGATCATCTGTATTCTGCACTAACTCGGAGAGGAATTCTCGCCTTTCGGGATGACCCAGAGCTTGAGAAAGGAAAATCCATTAGGCCTGAACTTTTTGCTGCAATTGAGGACTCTAGATCTGCGATTGTCATTCTCTCATGGAGATATGCTCGTTCGTCATGGTGCTTGGATGAACTCGTCAAGATTATTCAATGCATGAAAGAGATGGGCCAACAAGTCCTCCCCCTCTTCTACTGGGTGGATCCTTCTGATGTGCGGCACCACAGGGGAGTTTTTGAGCTTAAAAGGGAACCGGAAGTAGAAGTAAGGGAACATGAAGAAGTTTATGGGAAGGATGAGGACAGACTAAGTGCGTGGAGAGCTGCTTTGACTGAGGTGGCCAATCTTTCTGGCTGGCATTTAAGGGAACATGATCCACCTACAACAATACTAATTGACGCAATTGTTAATCATATACTAAAGAAGTCCGTGTATACATCTTCAAGTGTTGACAATGGCTTCATAGGAATGGGTTCCCGCATTGATGATTTATTAAGCAATTACATATATACGCAGCGTGGTAGGGTGCGTTTTATAGGGATCCATGGCATGCGGGGCATAGGTAAGACAGCACTTGCTCGAGCTATCCATGATGAAATTTGTCTGGATTTTGACCGAACCTGCTTTCTTTCCAATGTTAGAGAAATGTCTAAAAACAATGGCCTAGTTTCTCTACAAGAAAAACTTCTTTCCAGAATCCTGATGACAAAAATTGAAAATATAGAGGATGAATACACAGGAGCTGCTATGATACAAAGGCGGTTGTGTAGAATGAAGGTGCTTGTTGTTATTGATGATGTGGATCAGTTGACGCAATTACAGAAATTGGCTGGAAGTTGCAGCTGGTTTGGTCCAGGGACTAGAATTATCATAACCACCACAGATGTTCAGTTGTTAAAGGCACATGATGTTGATGCTACATACAAGGTTAACGAGTTAAACTGTGATGAAGCACTTCAACTTTTGAGTTTGAAGGCTTTTAAGAAATGGCCCCCACCAGAAGATTATTTGCATCTGTGCTACGATATTTTAGGGTATGCTCAGGGGCTTCCGTTGGCTCTCGTGGTTTTAGGTTCTTTTCTGTCAGGTAGAGGGGCTGATAAATGGGCAAGTGCAATAGATAGGCTGAGAAGCACACCAGATAAACGCATTATTGACGTGCTTCGGATTAGTTTTGATGACCTGCATAAAAAAGAGAAAGAAATATTCCTACATATTGCTTGCTTTTACAAGGGGAAGGATAAGGATCGAGTGACACAAATACTAGACTATTGCCAGCTACAACCTGTCATTGGTTTAGATGTTCTTGCTGATAGATCTCTCATAACTATCTCCAACAACGATTTGTCGATGCATGATTTGCTACAAGAAATGGGCTGGGAAGTTGTTCGTGAACAGTCTCCTGATGAGCCAGGCAAACGTAGTAGATTATGGTCTCCTGACGACATCAACGAAGTGCTGAAGAAAAATAAGGGAACAGATTCAATCCAAGGCATGGTAATGGAGTTGACTAAATTACAAGCGGCTCATTGGAAGCCAGAAGCCTTTTCAAATTTGTCTCAACTTAGTCTTCTCCATATTCGTAATGTGGACCTTCCCAAAGGCCTCACTTGTCTTTCCAAATCCTTGAGACTCCTCGAATGGATTGGGTGTCCCTTAAGATCTCTCCCACAAAACTTTGAAGCAGATGAACTTATTGAACTTAACTTGTGCCACAGCACCATTAAACAGCTTTGGAAGGGAGCAAAGAATTTTGGCAAGTTGAAGTTCATCAAACTCTGCCATTCTCAAAAAATTGTGGAGACCCCCGACCTCGCAGGTGTTCAGAATCTTGAGACTTTAGATCTTGAAGGATGTTCTCATTTGGTAAGAATCCATCAATCCCTTGGATTTCTCAAAAAGCTTATTGTCCTGAATCTTAAAGACTGCAAAAACCTCAAGAGTCTGCCAAGTAGAATTGAAATGGAATCTCTTGAAACATTAATTCTTTCTAACTGCTCAAAAGTTAAGAAGATTCCCGAGTTTGTTGGAAATATGGAACGTTTGTTGGTGCTTTGTCTTGATGAGACTGCCATTGAGGAACTGCCTGTTTCAATTGAACGGCTGACTGGCCTTGTGACATTGAATCTAAGCAACTGCAGAAATCTTGTTTGTCTTCCAAGCACCATCAATAAATTGAAGTCTGTTGAAAATCTTAATCTTTCTGGATGCTTGAAACTTGGCAAACATCAGGTAAATGTGGGGGGGATGGACTGTTTTGAGGAAACTGACGTGAATAGTGGGTCTGCAATGGAAATGTCATCTACCCATGACCGCATAAAATATGTGAGAGGTTCAATCTTTCATAGATGCAAAGTGGTTTGGGGATCTTTAAATAAGTTATTGCCTTCTGGATTGGTGCAAAAAGTGAATACAGAGCCAACGATTTTCCGCTTGCCTATTTCTGGTCTGTGTAATTTAACGTATCTGAACCTGAGTAATTGCAATCTTGGTGAAGGAGCATTTGCCGACGAATTCGGTTACTTTCCCTCTTTGGTGACCTTGAATCTAAGTGGAAACAATTTTGTTCGTGTTCCTTCAGGCATTCGATTGCTTTCTAAGCTTGAGAACTTTAACTTGGAGAATTGCAAGAGACTTCAAGAGTTGTCAGACCTTCCATCAAATAGTAGACTAGATTTAAGGGCAGATGGTTGTACTTCACTGAAATACTTGTTTGATGCATCAAATTTGAACAGATTAAACAAATCGTATTTCAATTTCATCAATTGCTTCAATCTAAATGGCAATCAAGGATGCAACAACATAGCATTTGAAATGCTGAAGACATTCATGTATCAGGGAATCTCTAATAAGAGGGAAACTTTTCAAATTGTAATTCCTGGAAGTAATATTCCTGAGTGGTTCAGCCATCAGAGTGTTGGGTGTTCATTAAGTGTATCTCTACCTGTACGTTGGAATAACAGTCAGGTTTTGGGATTTGCTCTGTGTGCTGTTTTTGTACTCCATGAGCATCATCAGGTGGATAAGCTTTATATAGATGAATTCAAGACTTTTAATGCAACACATCATCTTGTATGTTGCCTGAAGCTTGATGGAAGAGAATTGGGAGTATATGGCAGACAGCCTGCATTTCGCTTTAGTGAAGAATTTTGCCAGGTTGAATCAGATCACCTGTGGCTATTCTATGTATCTCGTGATAAATACTTTGGTACAGAGTCGTGGCATAACAGTTGCAGTCAGTTTGAGTTCTTATTTGAAACCAGAGGGCCGGGTCTGAAGGTGAAGGAGTGTGGAGTCCGTCTGATATATAAGCAAGATGTGCAAGAGTTGAACCAAACAACCACTCAATCAAGCAGTGGGATGTCTCCGTATGAGGATATATTGATTGGTTTTGACATTCCAGTTGCAGGGGAAACCAGTGGCACTGGTAGCACAACTTGCACGCTTGAAGAATTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005681 GO:0005684 GO:0005688 GO:0005689 GO:0006139 GO:0006396 GO:0006725 GO:0006807 GO:0008150 GO:0008152 GO:0009987 GO:0010467 GO:0016070 GO:0030532 GO:0032991 GO:0034641 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0046483 GO:0071004 GO:0071010 GO:0071013 GO:0071704 GO:0090304 GO:0097525 GO:0097526 GO:0120114 GO:1901360 GO:1902494 GO:1990726 GO:1990904

KEGG Pathways

Metabolic & Signaling

ko03018 ko03040 map03018 map03040

Pfam Domains

Protein Families

LSM

Protein Analysis

1193

Amino Acids

135.29

Weight (kDa)

6.55

Isoelectric Point (pI)

41.41

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
TIR	PF01582	11 - 190	2.2e-43	TIR domain
TIR_2	PF13676	14 - 107	5.6e-12	TIR domain
NB-ARC	PF00931	215 - 370	6.3e-18	NB-ARC domain
NACHT	PF05729	220 - 362	2.8e-06	NACHT domain
WHD_ROQ1	PF23282	438 - 508	1.9e-21	Disease resistance protein Roq1-like, winged-helix domain
LRR_13	PF23286	697 - 781	8.8e-11	Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14	PF23598	708 - 776	1.6e-08	Leucine-rich repeat region
C-JID	PF20160	1000 - 1149	3.5e-20	C-JID domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0008526)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G03870 AT2G03870
fragaria_vesca	FvH4_7g00800 FvH4_7g00800
malus_domestica	MD02G1314900.v1.1 MD07G1007200.v1.1
prunus_persica	Prupe.2G010100_v2.0.a1 Prupe.2G010100_v2.0.a1 Prupe.2G010100_v2.0.a1
pyrus_communis	pycom02g26390 pycom07g00570
rosa_chinensis	RchiOBHm_Chr1g0314961
rosa_multiflora	Rmu_sc0005168.1_g000014
rosa_roxburghii	Rroxscaffold_4G00331640
rosa_rugosa	Rorug01G0003600 Rorug01G0003700 Rorug01G0003800 Rorug01G0003900
rosa_samantha	Rh1AG014300 Rh1BG006100 Rh1CG012400 Rh1DG007900
rosa_wichuraiana	Rw1G000680

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1931
Acc36I	ACCTGC	3 cut(s)	752, 1314, 1931
AccB1I	GGYRCC	1 cut(s)	342
AccB7I	CCANNNNNTGG	1 cut(s)	1618
AccI	GTMKAC	2 cut(s)	557, 2791
AciI	CCGC	8 cut(s)	70, 340, 601, 676, 883, 1646, 2437, 2554
AclI	AACGTT	1 cut(s)	2164
AclWI	GGATC	8 cut(s)	320, 333, 491, 658, 671, 933, 1377, 2497
AcoI	YGGCCR	1 cut(s)	462
AcsI	RAATTY	8 cut(s)	117, 720, 1675, 1873, 2636, 2848, 3164, 3269
AcuI	CTGAAG	5 cut(s)	1604, 2685, 2963, 3227, 3419
AcyI	GRCGYC	1 cut(s)	5
AfaI	GTAC	4 cut(s)	2818, 3072, 3123, 3334
AfiI	CCNNNNNNNGG	4 cut(s)	1415, 1618, 2567, 3530
AflII	CTTAAG	1 cut(s)	1780
AjiI	CACGTC	2 cut(s)	1282, 2395
AjnI	CCWGG	4 cut(s)	976, 1534, 3001, 3276
AjuI	GAANNNNNNNTTGG	2 cut(s)	2723, 2755
Alw26I	GTCTC	9 cut(s)	1527, 1563, 1686, 1747, 1919, 1952, 2183, 2749, 3492
AlwI	GGATC	8 cut(s)	320, 333, 491, 658, 671, 933, 1377, 2497
AlwNI	CAGNNNCTG	1 cut(s)	866
Ama87I	CYCGRG	2 cut(s)	702, 2141
AoxI	GGCC	8 cut(s)	167, 292, 462, 784, 1112, 1726, 2233, 3389
ApeKI	GCWGC	5 cut(s)	182, 446, 637, 866, 963
ApoI	RAATTY	8 cut(s)	117, 720, 1675, 1873, 2636, 2848, 3164, 3269
AseI	ATTAAT	1 cut(s)	2105
Asp700I	GAANNNNTTC	7 cut(s)	595, 807, 1077, 1509, 1603, 2948, 2985
AspS9I	GGNCC	5 cut(s)	292, 974, 1113, 1714, 3389
AsuC2I	CCSGG	1 cut(s)	3393
AsuHPI	GGTGA	4 cut(s)	2627, 2671, 3284, 3415
AvaI	CYCGRG	2 cut(s)	702, 2141
AvaII	GGWCC	2 cut(s)	974, 1714
BalI	TGGCCA	1 cut(s)	464
BamHI	GGATCC	2 cut(s)	325, 663
BanI	GGYRCC	1 cut(s)	342
BauI	CACGAG	2 cut(s)	1184, 3315
BbsI	GAAGAC	3 cut(s)	1685, 2270, 2951
BbvI	GCAGC	5 cut(s)	169, 433, 649, 853, 975
BccI	CCATC	8 cut(s)	283, 2007, 2297, 2365, 2788, 2804, 3039, 3209
BceAI	ACGGC	1 cut(s)	2240
BciT130I	CCWGG	4 cut(s)	978, 1536, 3003, 3278
BcnI	CCSGG	1 cut(s)	3393
BcoDI	GTCTC	9 cut(s)	1527, 1563, 1686, 1747, 1919, 1952, 2183, 2749, 3492
BfaI	CTAG	5 cut(s)	198, 788, 984, 1388, 2795
BfmI	CTRYAG	1 cut(s)	2260
BfrI	CTTAAG	1 cut(s)	1780
BfuAI	ACCTGC	3 cut(s)	752, 1314, 1931
BglII	AGATCT	4 cut(s)	200, 1439, 1784, 1966
BisI	GCNGC	7 cut(s)	183, 341, 447, 638, 867, 964, 1647
BlsI	GCNGC	7 cut(s)	184, 342, 448, 639, 868, 965, 1648
Bme1390I	CCNGG	5 cut(s)	978, 1536, 3003, 3278, 3393
Bme18I	GGWCC	2 cut(s)	974, 1714
BmeT110I	CYCGRG	2 cut(s)	702, 2141
BmgBI	CACGTC	2 cut(s)	1282, 2395
BmgT120I	GGNCC	5 cut(s)	292, 974, 1113, 1714, 3389
BmiI	GGNNCC	6 cut(s)	327, 344, 375, 598, 665, 1115
BmrFI	CCNGG	5 cut(s)	978, 1536, 3003, 3278, 3393
BmrI	ACTGGG	1 cut(s)	328
BmsI	GCATC	9 cut(s)	1027, 1144, 1464, 2321, 2462, 2830, 2852, 2906, 3142
BmuI	ACTGGG	1 cut(s)	328
BpiI	GAAGAC	3 cut(s)	1685, 2270, 2951
BplI	GAGNNNNNCTC	4 cut(s)	105, 137, 217, 249
Bpu10I	CCTNAGC	1 cut(s)	1164
BpuEI	CTTGAG	5 cut(s)	167, 1771, 1976, 2045, 2747
BpuMI	CCSGG	1 cut(s)	3393
BsaBI	GATNNNNATC	2 cut(s)	924, 1368
BsaHI	GRCGYC	1 cut(s)	5
BsaI	GGTCTC	2 cut(s)	1563, 1919
BsaJI	CCNNGG	4 cut(s)	667, 977, 1612, 2008
BsaWI	WCCGGW	1 cut(s)	376
BsaXI	ACNNNNNCTCC	2 cut(s)	288, 318
Bsc4I	CCNNNNNNNGG	4 cut(s)	1415, 1618, 2567, 3530
Bse1I	ACTGG	5 cut(s)	323, 2236, 3524, 3540, 3553
Bse3DI	GCAATG	1 cut(s)	2418
Bse8I	GATNNNNATC	2 cut(s)	924, 1368
BseBI	CCWGG	4 cut(s)	978, 1536, 3003, 3278
BseDI	CCNNGG	4 cut(s)	667, 977, 1612, 2008
BseGI	GGATG	9 cut(s)	139, 259, 424, 856, 1982, 2336, 2376, 2921, 3489
BseJI	GATNNNNATC	2 cut(s)	924, 1368
BseLI	CCNNNNNNNGG	4 cut(s)	1415, 1618, 2567, 3530
BseMI	GCAATG	1 cut(s)	2418
BseMII	CTCAG	5 cut(s)	447, 1178, 1241, 2588, 3009
BseNI	ACTGG	5 cut(s)	323, 2236, 3524, 3540, 3553
BseRI	GAGGAG	1 cut(s)	1748
BseXI	GCAGC	5 cut(s)	169, 433, 649, 853, 975
BseYI	CCCAGC	1 cut(s)	1500
BsgI	GTGCAG	1 cut(s)	84
BshFI	GGCC	8 cut(s)	169, 294, 464, 786, 1114, 1728, 2235, 3391
BshNI	GGYRCC	1 cut(s)	342
BsiHKCI	CYCGRG	2 cut(s)	702, 2141
BsiSI	CCGG	2 cut(s)	377, 3392
BslFI	GGGAC	2 cut(s)	994, 1761
BslI	CCNNNNNNNGG	4 cut(s)	1415, 1618, 2567, 3530
BsmAI	GTCTC	9 cut(s)	1527, 1563, 1686, 1747, 1919, 1952, 2183, 2749, 3492
BsmFI	GGGAC	2 cut(s)	994, 1761
BsmI	GAATGC	1 cut(s)	2706
BsnI	GGCC	8 cut(s)	169, 294, 464, 786, 1114, 1728, 2235, 3391
Bso31I	GGTCTC	2 cut(s)	1563, 1919
BsoBI	CYCGRG	2 cut(s)	702, 2141
Bsp19I	CCATGG	1 cut(s)	667
BspACI	CCGC	8 cut(s)	70, 340, 601, 676, 883, 1646, 2437, 2554
BspANI	GGCC	8 cut(s)	169, 294, 464, 786, 1114, 1728, 2235, 3391
BspCNI	CTCAG	5 cut(s)	448, 1177, 1242, 2589, 3010
BspLI	GGNNCC	6 cut(s)	327, 344, 375, 598, 665, 1115
BspMAI	CTGCAG	1 cut(s)	2264
BspMI	ACCTGC	3 cut(s)	752, 1314, 1931
BspPI	GGATC	8 cut(s)	320, 333, 491, 658, 671, 933, 1377, 2497
BspT107I	GGYRCC	1 cut(s)	342
BspTI	CTTAAG	1 cut(s)	1780
BspTNI	GGTCTC	2 cut(s)	1563, 1919
BsrDI	GCAATG	1 cut(s)	2418
BsrI	ACTGG	5 cut(s)	323, 2236, 3524, 3540, 3553
BssECI	CCNNGG	4 cut(s)	667, 977, 1612, 2008
BssNAI	GTATAC	1 cut(s)	558
BssNI	GRCGYC	1 cut(s)	5
BssSI	CACGAG	2 cut(s)	1184, 3315
BssT1I	CCWWGG	3 cut(s)	667, 1612, 2008
Bst1107I	GTATAC	1 cut(s)	558
Bst2BI	CACGAG	2 cut(s)	1184, 3315
Bst2UI	CCWGG	4 cut(s)	978, 1536, 3003, 3278
Bst4CI	ACNGT	5 cut(s)	1067, 1521, 2378, 3086, 3353
Bst6I	CTCTTC	3 cut(s)	19, 317, 3214
BstACI	GRCGYC	1 cut(s)	5
BstAFI	CTTAAG	1 cut(s)	1780
BstAPI	GCANNNNNTGC	3 cut(s)	1483, 3442, 3561
BstC8I	GCNNGC	7 cut(s)	479, 674, 1345, 1400, 2558, 3249, 3566
BstDSI	CCRYGG	1 cut(s)	667
BstEII	GGTNACC	1 cut(s)	2659
BstF5I	GGATG	9 cut(s)	139, 259, 424, 856, 1982, 2336, 2376, 2921, 3489
BstMAI	GTCTC	9 cut(s)	1527, 1563, 1686, 1747, 1919, 1952, 2183, 2749, 3492
BstMWI	GCNNNNNNNGC	8 cut(s)	443, 678, 1483, 2722, 3110, 3442, 3552, 3561
BstNI	CCWGG	4 cut(s)	978, 1536, 3003, 3278
BstNSI	RCATGY	1 cut(s)	676
BstPI	GGTNACC	1 cut(s)	2659
BstSCI	CCNGG	5 cut(s)	976, 1534, 3001, 3276, 3391
BstSFI	CTRYAG	1 cut(s)	2260
BstV1I	GCAGC	5 cut(s)	169, 433, 649, 853, 975
BstV2I	GAAGAC	3 cut(s)	1685, 2270, 2951
BstX2I	RGATCY	7 cut(s)	200, 325, 663, 1439, 1784, 1966, 2489
BstYI	RGATCY	7 cut(s)	200, 325, 663, 1439, 1784, 1966, 2489
BstZ17I	GTATAC	1 cut(s)	558
BsuRI	GGCC	8 cut(s)	169, 294, 464, 786, 1114, 1728, 2235, 3391
BtgI	CCRYGG	1 cut(s)	667
BtrI	CACGTC	2 cut(s)	1282, 2395
BtsCI	GGATG	9 cut(s)	139, 259, 424, 856, 1982, 2336, 2376, 2921, 3489
BtsI	GCAGTG	2 cut(s)	79, 3484
BtsIMutI	CAGTG	5 cut(s)	79, 2822, 3484, 3546, 3547
BveI	ACCTGC	3 cut(s)	752, 1314, 1931
Cac8I	GCNNGC	7 cut(s)	479, 674, 1345, 1400, 2558, 3249, 3566
CaiI	CAGNNNCTG	1 cut(s)	866
Cfr13I	GGNCC	5 cut(s)	292, 974, 1113, 1714, 3389
CseI	GACGC	2 cut(s)	530, 943
Csp6I	GTAC	4 cut(s)	2817, 3071, 3122, 3333
CviQI	GTAC	4 cut(s)	2817, 3071, 3122, 3333
DraI	TTTAAA	1 cut(s)	2496
EaeI	YGGCCR	1 cut(s)	462
Eam1104I	CTCTTC	3 cut(s)	19, 317, 3214
EarI	CTCTTC	3 cut(s)	19, 317, 3214
Eco130I	CCWWGG	3 cut(s)	667, 1612, 2008
Eco147I	AGGCCT	2 cut(s)	169, 1728
Eco31I	GGTCTC	2 cut(s)	1563, 1919
Eco47I	GGWCC	2 cut(s)	974, 1714
Eco57I	CTGAAG	5 cut(s)	1604, 2685, 2963, 3227, 3419
Eco88I	CYCGRG	2 cut(s)	702, 2141
Eco91I	GGTNACC	1 cut(s)	2659
EcoO65I	GGTNACC	1 cut(s)	2659
EcoRI	GAATTC	3 cut(s)	117, 2636, 3164
EcoRII	CCWGG	4 cut(s)	976, 1534, 3001, 3276
EcoT14I	CCWWGG	3 cut(s)	667, 1612, 2008
EcoT22I	ATGCAT	3 cut(s)	281, 1479, 2845
ErhI	CCWWGG	3 cut(s)	667, 1612, 2008
FalI	AAGNNNNNCTT	2 cut(s)	792, 824
FaqI	GGGAC	2 cut(s)	994, 1761
FauI	CCCGC	3 cut(s)	77, 608, 669
FblI	GTMKAC	2 cut(s)	557, 2791
Fnu4HI	GCNGC	7 cut(s)	183, 341, 447, 638, 867, 964, 1647
FokI	GGATG	9 cut(s)	146, 266, 431, 863, 1989, 2343, 2383, 2928, 3496
Fsp4HI	GCNGC	7 cut(s)	183, 341, 447, 638, 867, 964, 1647
FspBI	CTAG	5 cut(s)	198, 788, 984, 1388, 2795
GluI	GCNGC	7 cut(s)	183, 341, 447, 638, 867, 964, 1647
GsaI	CCCAGC	1 cut(s)	1504
HaeIII	GGCC	8 cut(s)	169, 294, 464, 786, 1114, 1728, 2235, 3391
HapII	CCGG	2 cut(s)	377, 3392
HgaI	GACGC	2 cut(s)	530, 943
Hin1I	GRCGYC	1 cut(s)	5
HincII	GTYRAC	5 cut(s)	9, 574, 933, 1054, 1632
HindII	GTYRAC	5 cut(s)	9, 574, 933, 1054, 1632
HindIII	AAGCTT	4 cut(s)	2024, 2723, 3149, 3209
HpaI	GTTAAC	1 cut(s)	1054
HpaII	CCGG	2 cut(s)	377, 3392
HphI	GGTGA	4 cut(s)	2627, 2671, 3284, 3415
Hpy166II	GTNNAC	9 cut(s)	9, 558, 574, 933, 1054, 1517, 1632, 1714, 2792
Hpy8I	GTNNAC	9 cut(s)	9, 558, 574, 933, 1054, 1517, 1632, 1714, 2792
Hpy99I	CGWCG	2 cut(s)	24, 2636
HpyCH4III	ACNGT	5 cut(s)	1067, 1521, 2378, 3086, 3353
HpyCH4IV	ACGT	6 cut(s)	1281, 1543, 2164, 2394, 2585, 3073
HpyF10VI	GCNNNNNNNGC	8 cut(s)	443, 678, 1483, 2722, 3110, 3442, 3552, 3561
HpySE526I	ACGT	6 cut(s)	1281, 1543, 2164, 2394, 2585, 3073
Hsp92I	GRCGYC	1 cut(s)	5
KspAI	GTTAAC	1 cut(s)	1054
LmnI	GCTCC	3 cut(s)	863, 1865, 2621
Lsp1109I	GCAGC	5 cut(s)	169, 433, 649, 853, 975
LweI	GCATC	9 cut(s)	1027, 1144, 1464, 2321, 2462, 2830, 2852, 2906, 3142
MaeI	CTAG	5 cut(s)	198, 788, 984, 1388, 2795
MaeII	ACGT	6 cut(s)	1281, 1543, 2164, 2394, 2585, 3073
MaeIII	GTNAC	4 cut(s)	1375, 2239, 2642, 2659
MfeI	CAATTG	4 cut(s)	186, 525, 2217, 2887
MflI	RGATCY	7 cut(s)	200, 325, 663, 1439, 1784, 1966, 2489
MlsI	TGGCCA	1 cut(s)	464
MluNI	TGGCCA	1 cut(s)	464
MlyI	GAGTC	5 cut(s)	188, 1749, 2074, 3347, 3426
MmeI	TCCRAC	3 cut(s)	1482, 2131, 3056
Mox20I	TGGCCA	1 cut(s)	464
Mph1103I	ATGCAT	3 cut(s)	281, 1479, 2845
MroXI	GAANNNNTTC	7 cut(s)	595, 807, 1077, 1509, 1603, 2948, 2985
MscI	TGGCCA	1 cut(s)	464
MslI	CAYNNNNRTG	3 cut(s)	590, 1622, 3036
Msp20I	TGGCCA	1 cut(s)	464
MspA1I	CMGCKG	1 cut(s)	966
MspCI	CTTAAG	1 cut(s)	1780
MspI	CCGG	2 cut(s)	377, 3392
MspR9I	CCNGG	5 cut(s)	978, 1536, 3003, 3278, 3393
MunI	CAATTG	4 cut(s)	186, 525, 2217, 2887
Mva1269I	GAATGC	1 cut(s)	2706
MvaI	CCWGG	4 cut(s)	978, 1536, 3003, 3278
MwoI	GCNNNNNNNGC	8 cut(s)	443, 678, 1483, 2722, 3110, 3442, 3552, 3561
NciI	CCSGG	1 cut(s)	3393
NcoI	CCATGG	1 cut(s)	667
NlaIV	GGNNCC	6 cut(s)	327, 344, 375, 598, 665, 1115
NmuCI	GTSAC	3 cut(s)	1375, 2239, 2659
NsiI	ATGCAT	3 cut(s)	281, 1479, 2845
NspI	RCATGY	1 cut(s)	676
PaeI	GCATGC	1 cut(s)	676
PaeR7I	CTCGAG	1 cut(s)	702
PaqCI	CACCTGC	1 cut(s)	1931
PceI	AGGCCT	2 cut(s)	169, 1728
PcsI	WCGNNNNNNNCGW	1 cut(s)	1572
PctI	GAATGC	1 cut(s)	2706
PdmI	GAANNNNTTC	7 cut(s)	595, 807, 1077, 1509, 1603, 2948, 2985
PflMI	CCANNNNNTGG	1 cut(s)	1618
PfoI	TCCNGGA	1 cut(s)	3001
PkrI	GCNGC	7 cut(s)	184, 342, 448, 639, 868, 965, 1648
PleI	GAGTC	5 cut(s)	188, 1749, 2073, 3346, 3425
PpsI	GAGTC	5 cut(s)	188, 1749, 2073, 3346, 3425
PshBI	ATTAAT	1 cut(s)	2105
Psp1406I	AACGTT	1 cut(s)	2164
Psp6I	CCWGG	4 cut(s)	976, 1534, 3001, 3276
PspEI	GGTNACC	1 cut(s)	2659
PspFI	CCCAGC	1 cut(s)	1500
PspGI	CCWGG	4 cut(s)	976, 1534, 3001, 3276
PspN4I	GGNNCC	6 cut(s)	327, 344, 375, 598, 665, 1115
PspPI	GGNCC	5 cut(s)	292, 974, 1113, 1714, 3389
PspXI	VCTCGAGB	1 cut(s)	702
PstI	CTGCAG	1 cut(s)	2264
PstNI	CAGNNNCTG	1 cut(s)	866
PsuI	RGATCY	7 cut(s)	200, 325, 663, 1439, 1784, 1966, 2489
PvuII	CAGCTG	1 cut(s)	966
RsaI	GTAC	4 cut(s)	2818, 3072, 3123, 3334
RsaNI	GTAC	4 cut(s)	2817, 3071, 3122, 3333
RseI	CAYNNNNRTG	3 cut(s)	590, 1622, 3036
SatI	GCNGC	7 cut(s)	183, 341, 447, 638, 867, 964, 1647
Sau96I	GGNCC	5 cut(s)	292, 974, 1113, 1714, 3389
SchI	GAGTC	5 cut(s)	188, 1749, 2074, 3347, 3426
ScrFI	CCNGG	5 cut(s)	978, 1536, 3003, 3278, 3393
SfaNI	GCATC	9 cut(s)	1027, 1144, 1464, 2321, 2462, 2830, 2852, 2906, 3142
SfcI	CTRYAG	1 cut(s)	2260
Sfr274I	CTCGAG	1 cut(s)	702
SinI	GGWCC	2 cut(s)	974, 1714
SlaI	CTCGAG	1 cut(s)	702
SmiMI	CAYNNNNRTG	3 cut(s)	590, 1622, 3036
SmlI	CTYRAG	7 cut(s)	146, 702, 1750, 1780, 1955, 2060, 2726
SmoI	CTYRAG	7 cut(s)	146, 702, 1750, 1780, 1955, 2060, 2726
SphI	GCATGC	1 cut(s)	676
SseBI	AGGCCT	2 cut(s)	169, 1728
SsiI	CCGC	8 cut(s)	70, 340, 601, 676, 883, 1646, 2437, 2554
SspI	AATATT	2 cut(s)	1329, 3013
SspMI	CTAG	5 cut(s)	198, 788, 984, 1388, 2795
StuI	AGGCCT	2 cut(s)	169, 1728
StyD4I	CCNGG	5 cut(s)	976, 1534, 3001, 3276, 3391
StyI	CCWWGG	3 cut(s)	667, 1612, 2008
TaaI	ACNGT	5 cut(s)	1067, 1521, 2378, 3086, 3353
TaiI	ACGT	6 cut(s)	1284, 1546, 2167, 2397, 2588, 3076
TaqI	TCGA	5 cut(s)	703, 1372, 1472, 1761, 2710
TaqII	GACCGA	1 cut(s)	753
TatI	WGTACW	2 cut(s)	2816, 3121
TauI	GCSGC	2 cut(s)	343, 1649
TscAI	CASTG	5 cut(s)	79, 2829, 3484, 3547, 3553
TseFI	GTSAC	3 cut(s)	1375, 2239, 2659
TseI	GCWGC	5 cut(s)	182, 446, 637, 866, 963
Tsp45I	GTSAC	3 cut(s)	1375, 2239, 2659
TspGWI	ACGGA	4 cut(s)	541, 1164, 3410, 3480
TspRI	CASTG	5 cut(s)	79, 2829, 3484, 3547, 3553
Van91I	CCANNNNNTGG	1 cut(s)	1618
Vha464I	CTTAAG	1 cut(s)	1780
VpaK11BI	GGWCC	2 cut(s)	974, 1714
VspI	ATTAAT	1 cut(s)	2105
XapI	RAATTY	8 cut(s)	117, 720, 1675, 1873, 2636, 2848, 3164, 3269
XbaI	TCTAGA	1 cut(s)	197
XceI	RCATGY	1 cut(s)	676
XcmI	CCANNNNNNNNNTGG	2 cut(s)	2006, 3038
XhoI	CTCGAG	1 cut(s)	702
XmiI	GTMKAC	2 cut(s)	557, 2791
XmnI	GAANNNNTTC	7 cut(s)	595, 807, 1077, 1509, 1603, 2948, 2985
XspI	CTAG	5 cut(s)	198, 788, 984, 1388, 2795
Zsp2I	ATGCAT	3 cut(s)	281, 1479, 2845

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.