pycom07g00570

U6 snRNA-associated Sm-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr7
OG0008526
Physical Location & Seq
Forward (+)
527397 .. 530139
2743 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom07g00570.1

Sequence Viewer

Length: 294 bp
ATGTCAGGGAGGAAAGAAACAGTTTTGGACCTGGCCAAGTTTGTGGACAAAGGTGTCCAAGTCAAGCTCACCGGCGGCAGACAAGTGACAGGGACTCTGAAAGGATATGACCAGTTGTTAAACCTTGTCCTAGATGAAGCAGTGGAGTTTCTAAGAGCGATCACCCTGAAGACCACTGATCAGACCAGGCGCCTTGGCCTCATAGTTTGTAGGGGAACCGCTGTAATGCTTGTGTCGCCGACAGATGGTACAGATGAGATCGCCAACCCTTTTAGCCAGCCAGATGGTGCCTAA

Protein Analysis

98

Amino Acids

10.46

Weight (kDa)

6.18

Isoelectric Point (pI)

22.01

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LSM PF01423 10 - 80 1.2e-19 LSM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 53
AccB1I GGYRCC 2 cut(s) 189, 287
AciI CCGC 2 cut(s) 75, 219
AcoI YGGCCR 1 cut(s) 33
AcuI CTGAAG 1 cut(s) 188
AcyI GRCGYC 1 cut(s) 190
AfaI GTAC 1 cut(s) 250
AfiI CCNNNNNNNGG 1 cut(s) 245
AjnI CCWGG 2 cut(s) 30, 185
AluBI AGCT 1 cut(s) 67
AluI AGCT 1 cut(s) 67
AoxI GGCC 2 cut(s) 33, 196
AspLEI GCGC 1 cut(s) 192
AspS9I GGNCC 1 cut(s) 28
AsuHPI GGTGA 2 cut(s) 61, 154
AvaII GGWCC 1 cut(s) 28
BalI TGGCCA 1 cut(s) 35
BanI GGYRCC 2 cut(s) 189, 287
BbsI GAAGAC 1 cut(s) 176
BccI CCATC 2 cut(s) 239, 278
BciT130I CCWGG 2 cut(s) 32, 187
BclI TGATCA 1 cut(s) 178
BfaI CTAG 1 cut(s) 131
BfoI RGCGCY 1 cut(s) 193
BisI GCNGC 1 cut(s) 76
BlsI GCNGC 1 cut(s) 77
Bme1390I CCNGG 2 cut(s) 32, 187
Bme18I GGWCC 1 cut(s) 28
BmgT120I GGNCC 1 cut(s) 28
BmiI GGNNCC 3 cut(s) 191, 217, 289
BmrFI CCNGG 2 cut(s) 32, 187
BpiI GAAGAC 1 cut(s) 176
BsaHI GRCGYC 1 cut(s) 190
BsaJI CCNNGG 1 cut(s) 193
Bsc4I CCNNNNNNNGG 1 cut(s) 245
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 1 cut(s) 112
BseBI CCWGG 2 cut(s) 32, 187
BseDI CCNNGG 1 cut(s) 193
BseLI CCNNNNNNNGG 1 cut(s) 245
BseNI ACTGG 1 cut(s) 112
BshFI GGCC 2 cut(s) 35, 198
BshNI GGYRCC 2 cut(s) 189, 287
BsiSI CCGG 1 cut(s) 72
BslFI GGGAC 1 cut(s) 106
BslI CCNNNNNNNGG 1 cut(s) 245
BsmFI GGGAC 1 cut(s) 106
BsnI GGCC 2 cut(s) 35, 198
Bsp143I GATC 3 cut(s) 159, 178, 258
BspACI CCGC 2 cut(s) 75, 219
BspANI GGCC 2 cut(s) 35, 198
BspLI GGNNCC 3 cut(s) 191, 217, 289
BspT107I GGYRCC 2 cut(s) 189, 287
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 1 cut(s) 112
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 1 cut(s) 193
BssMI GATC 3 cut(s) 159, 178, 258
BssNI GRCGYC 1 cut(s) 190
BssT1I CCWWGG 1 cut(s) 193
Bst2UI CCWGG 2 cut(s) 32, 187
Bst4CI ACNGT 1 cut(s) 22
BstACI GRCGYC 1 cut(s) 190
BstC8I GCNNGC 1 cut(s) 278
BstDEI CTNAG 1 cut(s) 152
BstH2I RGCGCY 1 cut(s) 193
BstHHI GCGC 1 cut(s) 192
BstKTI GATC 3 cut(s) 162, 181, 261
BstMBI GATC 3 cut(s) 159, 178, 258
BstMWI GCNNNNNNNGC 1 cut(s) 235
BstNI CCWGG 2 cut(s) 32, 187
BstSCI CCNGG 2 cut(s) 30, 185
BstV2I GAAGAC 1 cut(s) 176
BstXI CCANNNNNNTGG 2 cut(s) 43, 284
BsuRI GGCC 2 cut(s) 35, 198
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 2 cut(s) 147, 174
Cac8I GCNNGC 1 cut(s) 278
CfoI GCGC 1 cut(s) 192
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 1 cut(s) 28
Csp6I GTAC 1 cut(s) 249
CviJI RGCY 5 cut(s) 35, 67, 198, 276, 280
CviKI_1 RGCY 5 cut(s) 35, 67, 198, 276, 280
CviQI GTAC 1 cut(s) 249
DdeI CTNAG 1 cut(s) 152
DinI GGCGCC 1 cut(s) 191
DpnI GATC 3 cut(s) 161, 180, 260
DpnII GATC 3 cut(s) 159, 178, 258
DrdI GACNNNNNNGTC 1 cut(s) 53
DseDI GACNNNNNNGTC 1 cut(s) 53
EaeI YGGCCR 1 cut(s) 33
Eco130I CCWWGG 1 cut(s) 193
Eco47I GGWCC 1 cut(s) 28
Eco57I CTGAAG 1 cut(s) 188
EcoRII CCWGG 2 cut(s) 30, 185
EcoT14I CCWWGG 1 cut(s) 193
EgeI GGCGCC 1 cut(s) 191
EheI GGCGCC 1 cut(s) 191
ErhI CCWWGG 1 cut(s) 193
FaiI YATR 2 cut(s) 108, 203
FaqI GGGAC 1 cut(s) 106
FbaI TGATCA 1 cut(s) 178
Fnu4HI GCNGC 1 cut(s) 76
Fsp4HI GCNGC 1 cut(s) 76
FspBI CTAG 1 cut(s) 131
GlaI GCGC 1 cut(s) 191
GluI GCNGC 1 cut(s) 76
HaeII RGCGCY 1 cut(s) 193
HaeIII GGCC 2 cut(s) 35, 198
HapII CCGG 1 cut(s) 72
HhaI GCGC 1 cut(s) 192
Hin1I GRCGYC 1 cut(s) 190
Hin6I GCGC 1 cut(s) 190
HinP1I GCGC 1 cut(s) 190
HinfI GANTC 1 cut(s) 94
HpaII CCGG 1 cut(s) 72
HphI GGTGA 2 cut(s) 61, 154
Hpy166II GTNNAC 1 cut(s) 46
Hpy188I TCNGA 2 cut(s) 99, 183
Hpy8I GTNNAC 1 cut(s) 46
HpyCH4III ACNGT 1 cut(s) 22
HpyF10VI GCNNNNNNNGC 1 cut(s) 235
HpyF3I CTNAG 1 cut(s) 152
Hsp92I GRCGYC 1 cut(s) 190
HspAI GCGC 1 cut(s) 190
KasI GGCGCC 1 cut(s) 189
Ksp22I TGATCA 1 cut(s) 178
Kzo9I GATC 3 cut(s) 159, 178, 258
LpnPI CCDG 9 cut(s) 17, 44, 75, 85, 125, 172, 179, 199, 290
MaeI CTAG 1 cut(s) 131
MaeIII GTNAC 1 cut(s) 85
MalI GATC 3 cut(s) 161, 180, 260
MboI GATC 3 cut(s) 159, 178, 258
MboII GAAGA 1 cut(s) 181
MlsI TGGCCA 1 cut(s) 35
MluNI TGGCCA 1 cut(s) 35
Mly113I GGCGCC 1 cut(s) 190
MlyI GAGTC 1 cut(s) 88
MnlI CCTC 2 cut(s) 3, 209
Mox20I TGGCCA 1 cut(s) 35
MscI TGGCCA 1 cut(s) 35
MseI TTAA 1 cut(s) 119
Msp20I TGGCCA 1 cut(s) 35
MspA1I CMGCKG 1 cut(s) 221
MspI CCGG 1 cut(s) 72
MspR9I CCNGG 2 cut(s) 32, 187
MvaI CCWGG 2 cut(s) 32, 187
MwoI GCNNNNNNNGC 1 cut(s) 235
NarI GGCGCC 1 cut(s) 190
NdeII GATC 3 cut(s) 159, 178, 258
NlaIV GGNNCC 3 cut(s) 191, 217, 289
NmuCI GTSAC 1 cut(s) 85
PkrI GCNGC 1 cut(s) 77
PleI GAGTC 1 cut(s) 88
PluTI GGCGCC 1 cut(s) 193
PpsI GAGTC 1 cut(s) 88
Psp6I CCWGG 2 cut(s) 30, 185
PspGI CCWGG 2 cut(s) 30, 185
PspN4I GGNNCC 3 cut(s) 191, 217, 289
PspPI GGNCC 1 cut(s) 28
RsaI GTAC 1 cut(s) 250
RsaNI GTAC 1 cut(s) 249
SaqAI TTAA 1 cut(s) 119
SatI GCNGC 1 cut(s) 76
Sau3AI GATC 3 cut(s) 159, 178, 258
Sau96I GGNCC 1 cut(s) 28
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 2 cut(s) 32, 187
SetI ASST 4 cut(s) 33, 55, 69, 126
SfoI GGCGCC 1 cut(s) 191
SgrAI CRCCGGYG 1 cut(s) 71
SinI GGWCC 1 cut(s) 28
SsiI CCGC 2 cut(s) 75, 219
SspDI GGCGCC 1 cut(s) 189
SspMI CTAG 1 cut(s) 131
StyD4I CCNGG 2 cut(s) 30, 185
StyI CCWWGG 1 cut(s) 193
TaaI ACNGT 1 cut(s) 22
TauI GCSGC 1 cut(s) 78
Tru1I TTAA 1 cut(s) 119
Tru9I TTAA 1 cut(s) 119
TscAI CASTG 2 cut(s) 147, 181
TseFI GTSAC 1 cut(s) 85
Tsp45I GTSAC 1 cut(s) 85
TspDTI ATGAA 1 cut(s) 150
TspRI CASTG 2 cut(s) 147, 181
VpaK11BI GGWCC 1 cut(s) 28
XspI CTAG 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.