MD07G1007200.v1.1

U6 snRNA-associated Sm-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0008526
Physical Location & Seq
Forward (+)
654536 .. 657895
3360 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1007200.v1.1.491

Sequence Viewer

Length: 300 bp
ATGTCAGGGAGGAAAGAAACAGTTTTGGACCTGGCCAAGTTTGTGGACAAAGGTGTCCAAGTCAAGCTCACCGGCGGGAGACAAGTGACAGGGACTCTGAAAGGATATGACCAGTTGTTAAACCTTGTCCTAGATGAAGCAGTGGAGTTTCTAAGAGATTCTGATGATCCATTGAAGACCACTGATCAGACCAGGCGCCTTGGCCTCATAGTTTGCAGGGGAACCGCTGTAATGCTTGTGTCGCCGACAGATGGTACAGATGAGATCGCCAACCCTTTTAGCCAGCCAGATGGTGCCTAA

Protein Analysis

100

Amino Acids

10.71

Weight (kDa)

4.79

Isoelectric Point (pI)

23.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LSM PF01423 10 - 82 8.4e-21 LSM domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 53
AccB1I GGYRCC 2 cut(s) 195, 293
AciI CCGC 2 cut(s) 75, 225
AclWI GGATC 1 cut(s) 161
AcoI YGGCCR 1 cut(s) 33
AcyI GRCGYC 1 cut(s) 196
AfaI GTAC 1 cut(s) 256
AfiI CCNNNNNNNGG 1 cut(s) 251
AgsI TTSAA 1 cut(s) 175
AjnI CCWGG 2 cut(s) 30, 191
AluBI AGCT 1 cut(s) 67
AluI AGCT 1 cut(s) 67
Alw26I GTCTC 1 cut(s) 73
AlwI GGATC 1 cut(s) 161
AoxI GGCC 2 cut(s) 33, 202
AspLEI GCGC 1 cut(s) 198
AspS9I GGNCC 1 cut(s) 28
AsuHPI GGTGA 1 cut(s) 61
AvaII GGWCC 1 cut(s) 28
BalI TGGCCA 1 cut(s) 35
BanI GGYRCC 2 cut(s) 195, 293
BbsI GAAGAC 1 cut(s) 182
BccI CCATC 2 cut(s) 245, 284
BciT130I CCWGG 2 cut(s) 32, 193
BclI TGATCA 1 cut(s) 184
BcoDI GTCTC 1 cut(s) 73
BfaI CTAG 1 cut(s) 131
BfoI RGCGCY 1 cut(s) 199
Bme1390I CCNGG 2 cut(s) 32, 193
Bme18I GGWCC 1 cut(s) 28
BmgT120I GGNCC 1 cut(s) 28
BmiI GGNNCC 3 cut(s) 197, 223, 295
BmrFI CCNGG 2 cut(s) 32, 193
BpiI GAAGAC 1 cut(s) 182
BsaHI GRCGYC 1 cut(s) 196
BsaJI CCNNGG 1 cut(s) 199
Bsc4I CCNNNNNNNGG 1 cut(s) 251
Bse118I RCCGGY 1 cut(s) 71
Bse1I ACTGG 1 cut(s) 112
BseBI CCWGG 2 cut(s) 32, 193
BseDI CCNNGG 1 cut(s) 199
BseLI CCNNNNNNNGG 1 cut(s) 251
BseNI ACTGG 1 cut(s) 112
BshFI GGCC 2 cut(s) 35, 204
BshNI GGYRCC 2 cut(s) 195, 293
BsiSI CCGG 1 cut(s) 72
BslFI GGGAC 1 cut(s) 106
BslI CCNNNNNNNGG 1 cut(s) 251
BsmAI GTCTC 1 cut(s) 73
BsmFI GGGAC 1 cut(s) 106
BsnI GGCC 2 cut(s) 35, 204
Bsp143I GATC 3 cut(s) 166, 184, 264
BspACI CCGC 2 cut(s) 75, 225
BspANI GGCC 2 cut(s) 35, 204
BspLI GGNNCC 3 cut(s) 197, 223, 295
BspPI GGATC 1 cut(s) 161
BspT107I GGYRCC 2 cut(s) 195, 293
BsrFI RCCGGY 1 cut(s) 71
BsrI ACTGG 1 cut(s) 112
BssAI RCCGGY 1 cut(s) 71
BssECI CCNNGG 1 cut(s) 199
BssMI GATC 3 cut(s) 166, 184, 264
BssNI GRCGYC 1 cut(s) 196
BssT1I CCWWGG 1 cut(s) 199
Bst2UI CCWGG 2 cut(s) 32, 193
Bst4CI ACNGT 1 cut(s) 22
BstACI GRCGYC 1 cut(s) 196
BstC8I GCNNGC 1 cut(s) 284
BstDEI CTNAG 1 cut(s) 152
BstH2I RGCGCY 1 cut(s) 199
BstHHI GCGC 1 cut(s) 198
BstKTI GATC 3 cut(s) 169, 187, 267
BstMAI GTCTC 1 cut(s) 73
BstMBI GATC 3 cut(s) 166, 184, 264
BstMWI GCNNNNNNNGC 1 cut(s) 241
BstNI CCWGG 2 cut(s) 32, 193
BstSCI CCNGG 2 cut(s) 30, 191
BstV2I GAAGAC 1 cut(s) 182
BstXI CCANNNNNNTGG 2 cut(s) 43, 290
BsuRI GGCC 2 cut(s) 35, 204
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 2 cut(s) 147, 180
Cac8I GCNNGC 1 cut(s) 284
CfoI GCGC 1 cut(s) 198
Cfr10I RCCGGY 1 cut(s) 71
Cfr13I GGNCC 1 cut(s) 28
Csp6I GTAC 1 cut(s) 255
CviJI RGCY 5 cut(s) 35, 67, 204, 282, 286
CviKI_1 RGCY 5 cut(s) 35, 67, 204, 282, 286
CviQI GTAC 1 cut(s) 255
DdeI CTNAG 1 cut(s) 152
DinI GGCGCC 1 cut(s) 197
DpnI GATC 3 cut(s) 168, 186, 266
DpnII GATC 3 cut(s) 166, 184, 264
DrdI GACNNNNNNGTC 1 cut(s) 53
DseDI GACNNNNNNGTC 1 cut(s) 53
EaeI YGGCCR 1 cut(s) 33
Eco130I CCWWGG 1 cut(s) 199
Eco47I GGWCC 1 cut(s) 28
EcoRII CCWGG 2 cut(s) 30, 191
EcoT14I CCWWGG 1 cut(s) 199
EgeI GGCGCC 1 cut(s) 197
EheI GGCGCC 1 cut(s) 197
ErhI CCWWGG 1 cut(s) 199
FaiI YATR 2 cut(s) 108, 209
FaqI GGGAC 1 cut(s) 106
FauI CCCGC 1 cut(s) 68
FbaI TGATCA 1 cut(s) 184
FspBI CTAG 1 cut(s) 131
GlaI GCGC 1 cut(s) 197
HaeII RGCGCY 1 cut(s) 199
HaeIII GGCC 2 cut(s) 35, 204
HapII CCGG 1 cut(s) 72
HhaI GCGC 1 cut(s) 198
Hin1I GRCGYC 1 cut(s) 196
Hin6I GCGC 1 cut(s) 196
HinP1I GCGC 1 cut(s) 196
HinfI GANTC 2 cut(s) 94, 158
HpaII CCGG 1 cut(s) 72
HphI GGTGA 1 cut(s) 61
Hpy166II GTNNAC 1 cut(s) 46
Hpy188I TCNGA 3 cut(s) 99, 163, 189
Hpy8I GTNNAC 1 cut(s) 46
HpyCH4III ACNGT 1 cut(s) 22
HpyCH4V TGCA 1 cut(s) 216
HpyF10VI GCNNNNNNNGC 1 cut(s) 241
HpyF3I CTNAG 1 cut(s) 152
Hsp92I GRCGYC 1 cut(s) 196
HspAI GCGC 1 cut(s) 196
KasI GGCGCC 1 cut(s) 195
Ksp22I TGATCA 1 cut(s) 184
Kzo9I GATC 3 cut(s) 166, 184, 264
LpnPI CCDG 9 cut(s) 17, 44, 75, 85, 125, 178, 202, 205, 296
MaeI CTAG 1 cut(s) 131
MaeIII GTNAC 1 cut(s) 85
MalI GATC 3 cut(s) 168, 186, 266
MboI GATC 3 cut(s) 166, 184, 264
MboII GAAGA 1 cut(s) 187
MlsI TGGCCA 1 cut(s) 35
MluNI TGGCCA 1 cut(s) 35
Mly113I GGCGCC 1 cut(s) 196
MlyI GAGTC 1 cut(s) 88
MnlI CCTC 2 cut(s) 3, 215
Mox20I TGGCCA 1 cut(s) 35
MscI TGGCCA 1 cut(s) 35
MseI TTAA 1 cut(s) 119
Msp20I TGGCCA 1 cut(s) 35
MspA1I CMGCKG 1 cut(s) 227
MspI CCGG 1 cut(s) 72
MspR9I CCNGG 2 cut(s) 32, 193
MvaI CCWGG 2 cut(s) 32, 193
MwoI GCNNNNNNNGC 1 cut(s) 241
NarI GGCGCC 1 cut(s) 196
NdeII GATC 3 cut(s) 166, 184, 264
NlaIV GGNNCC 3 cut(s) 197, 223, 295
NmuCI GTSAC 1 cut(s) 85
PfeI GAWTC 1 cut(s) 158
PleI GAGTC 1 cut(s) 88
PluTI GGCGCC 1 cut(s) 199
PpsI GAGTC 1 cut(s) 88
Psp6I CCWGG 2 cut(s) 30, 191
PspGI CCWGG 2 cut(s) 30, 191
PspN4I GGNNCC 3 cut(s) 197, 223, 295
PspPI GGNCC 1 cut(s) 28
RsaI GTAC 1 cut(s) 256
RsaNI GTAC 1 cut(s) 255
SaqAI TTAA 1 cut(s) 119
Sau3AI GATC 3 cut(s) 166, 184, 264
Sau96I GGNCC 1 cut(s) 28
SchI GAGTC 1 cut(s) 88
ScrFI CCNGG 2 cut(s) 32, 193
SetI ASST 4 cut(s) 33, 55, 69, 126
SfoI GGCGCC 1 cut(s) 197
SgrAI CRCCGGYG 1 cut(s) 71
SinI GGWCC 1 cut(s) 28
SsiI CCGC 2 cut(s) 75, 225
SspDI GGCGCC 1 cut(s) 195
SspMI CTAG 1 cut(s) 131
StyD4I CCNGG 2 cut(s) 30, 191
StyI CCWWGG 1 cut(s) 199
TaaI ACNGT 1 cut(s) 22
TfiI GAWTC 1 cut(s) 158
Tru1I TTAA 1 cut(s) 119
Tru9I TTAA 1 cut(s) 119
TscAI CASTG 2 cut(s) 147, 187
TseFI GTSAC 1 cut(s) 85
Tsp45I GTSAC 1 cut(s) 85
TspDTI ATGAA 1 cut(s) 150
TspRI CASTG 2 cut(s) 147, 187
VpaK11BI GGWCC 1 cut(s) 28
XspI CTAG 1 cut(s) 131
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.