MD03G1011500.v1.1

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0011667
Physical Location & Seq
Forward (+)
889383 .. 894488
5106 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1011500.v1.1.491

Sequence Viewer

Length: 2094 bp
ATGCCGGAGCTTCGCAGAGGAGTACGCCGAGGCCGCGCTAGGGTAGCAAGCAAGCCCTCCGACCTACCGCCGCGGTCGCGGAGGACCCGTGCCACCGTCGCCCGAGAAGCCGCCGCCGAGGCCGTGGTGAGGCCCAGGACGAGGTTGGCTGTGAGGAAATTGAAAGAAGAGGAGAAGCAGGAGCCGGATCCGGAGGAAGACCCTGTGATTGTGATATCCGTGAAAGACTCCGATTCGGAGGGCAGTAAGGAAGAAGAAGAAGTCGAGGAAGATAAGAAAGCTGTGATGGCTGACGATAGTGGTGGCCTGAGTGCTAATAAGGCTGCTGGGCAAGAAGAAGAGGGCAGTACTGCCCCTTTCCCCGAAAAGGTTCAAGTAGGAGGGTCACCACTCTATAAGGTAGAGAGGAAGCTGGGGAAAGGTGGCTTTGGTCAAGTGTTTGTTGGCCGTCGTGTTACGGGTGGAGTTGATCGTCTAAGTGGTGCTGGTGCTATTGAGGTAGCACTTAAATTCGAGCACAGAAATAGCAAAGGATGTACTTATGGTCCTCCATATGAGTGGCAAGTTTACAATACCCTTGGTGGTAGTCATGGAGTGCCAAAAGTACATTATAAAGGAAAGCAAGGAGACTATTATGTTATGGTTATGGACATGCTAGGGCCTAGTTTATGGGATGTATGGAATTCTTCAGGGCAAGCGATGTCTGCAGAAATGGTAGCTTGTATAGCTGTCGAGTCTTTATCAATTCTAGAGAAGATGCACTCAAGAGGCTATGTGCATGGAGATGTAAAGCCCGAAAATTTTTTACTTGGTCAGCCATCTACAGCTCAAGAGAAGAAGTTGTTTCTTGTTGACCTTGGACTAGCGACAAAGTGGAAAGACACTAGTGGGGGGCATGTTGATTATGATCAACGTCCTGATATGTTTAGGGGAACTGTGCGATATGCTAGTGTTCATGCTCATCTGGGAAGAACTGCTAGCAGAAGAGATGATCTTGAATCTCTTGCTTATACACTTATTTTTCTCCACCGAGGCAGGTTACCATGGCAAGGCTATCAGGGTGATAACAAATCTTTCCTAGTTTGCAAAAAAAAGATGGCAACATCTCCTGAAATGCTGTGCTGCTTCTGCCCCCCACCTCTAAGACAATTTCTTGAGGTCGTGGTGAACATGAAATTTGATGAAGAGCCTAACTATTCGAAGTTAATCTCGTTGTTTGAGGGTTTGATAGGATCAAATCCTGCTGTAAGGCCAATCAAAACTGATGGTGCTCAAAAGATTATCAGTCAGGTTGGCCAGAAACGGGGTAGATTGAATATTGAGGAAGATGATGATGGGCAGCCTAGGAAGAAGGTTCGGCTAGGAGTACCTGCCACCCAATGGATTTCAATTTACAATGCTAGGATGCCGATGAAACAGAGGTATCATTACAATGTCGCTGATGCGAGGTTGGCACAACATGTGGAGAGAGGGATTGCAGATGGTCTGCTTATAAGTTGTGTGTCATCTTGTTCTAATCTCTGGGCACTTATTATGGACGCTGGAACCGGTTTTACAAACCAAGTTTATGAGCTCTCCCCCTTCTTCTTGCACAAGGAATGGATCATGGAACAATGGGAGAAGAATTATTACATTAGTTCTATTGCCGGTGCTAACAATGGAAGTTCCCTTGTAGTGATGTCCAAAGGTACCCAGTATACGCAACAGTCTTACAAAGTCAGTGATTCTTTCCCCTTCAAGTGGATAAATAAGAAGTGGAGAGAAGGATTTCATGTGACCTCAATGGCAACTGCTGGGAGCCGGTGGGGTGTTGTTATGTCTCGCAATGCGGGCTTCAGTGATCAGGTTGTGGAGCTTGATTTTCTCTATCCGAGTGAAGGCATCCACAGACGTTGGGACAATGGTTTCCGCATAACATCGACAGCCGCAACTTGGGATCAAGCAGCTCTTATCTTGAGTGTTCCAAAGCGCAAACCTGGTGACGAAACTCAGGAGACGCTGCGCACGTCTCAATTCCCAAGCACACATGTGAAGGAAAAATGGGCGAAGAATCTGTATCTCGCTTGTTTGTGTTACGGGCGCACTGTATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04392 map04011 map04392
Pfam Domains
Protein Families
Pkinase

Protein Analysis

698

Amino Acids

78.0

Weight (kDa)

9.02

Isoelectric Point (pI)

49.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 132 - 417 1.3e-16 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 133 - 403 7.2e-07 Protein tyrosine and serine/threonine kinase
Pkinase_fungal PF17667 247 - 341 2.1e-07 Fungal protein kinase
DUF7477 PF24289 430 - 697 1.5e-162 Domain of unknown function (DUF7477)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011667)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13670
fragaria_vesca FvH4_3g44690
malus_domestica MD03G1011500.v1.1 MD11G1014200.v1.1
prunus_persica Prupe.6G011900_v2.0.a1 Prupe.6G011900_v2.0.a1 Prupe.6G011900_v2.0.a1
pyrus_communis pycom03g01040 pycom11g00960
rosa_chinensis RchiOBHm_Chr5g0081361
rosa_laevigata RLG00000036927
rosa_multiflora Rmu_sc0007768.1_g000010 Rmu_sc0009187.1_g000017
rosa_roxburghii Rroxscaffold_1G00002180
rosa_rugosa Rorug05G0476000
rosa_samantha Rh5AG527400 Rh5BG551600 Rh5CG574400
rosa_wichuraiana Rw5G049050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 612, 1493
Acc16I TGCGCA 1 cut(s) 2003
Acc36I ACCTGC 2 cut(s) 1026, 1378
Acc65I GGTACC 1 cut(s) 1688
AccB1I GGYRCC 1 cut(s) 1688
AccB7I CCANNNNNTGG 1 cut(s) 1380
AccI GTMKAC 1 cut(s) 1697
AccII CGCG 3 cut(s) 36, 73, 79
AccIII TCCGGA 1 cut(s) 190
AclWI GGATC 5 cut(s) 182, 195, 1240, 1610, 1944
AcoI YGGCCR 2 cut(s) 445, 1294
AcsI RAATTY 4 cut(s) 509, 682, 799, 1175
AcuI CTGAAG 2 cut(s) 672, 1819
AfaI GTAC 6 cut(s) 24, 349, 538, 606, 1368, 1690
AflIII ACRYGT 2 cut(s) 1459, 2026
AgeI ACCGGT 1 cut(s) 1547
AgsI TTSAA 6 cut(s) 163, 374, 998, 1315, 1389, 1738
AhlI ACTAGT 1 cut(s) 884
AjiI CACGTC 1 cut(s) 2007
AjnI CCWGG 2 cut(s) 134, 1975
AleI CACNNNNGTG 1 cut(s) 2027
AluBI AGCT 9 cut(s) 10, 281, 412, 719, 728, 827, 1573, 1855, 1946
AluI AGCT 9 cut(s) 10, 281, 412, 719, 728, 827, 1573, 1855, 1946
Alw21I GWGCWC 3 cut(s) 519, 1273, 1575
Alw26I GTCTC 4 cut(s) 621, 1824, 1988, 2013
AlwI GGATC 5 cut(s) 182, 195, 1240, 1610, 1944
Ama87I CYCGRG 1 cut(s) 102
Aor13HI TCCGGA 1 cut(s) 190
AoxI GGCC 8 cut(s) 31, 120, 131, 304, 445, 659, 1250, 1294
ApeKI GCWGC 5 cut(s) 323, 1122, 1339, 1943, 1999
ApoI RAATTY 4 cut(s) 509, 682, 799, 1175
AsiGI ACCGGT 1 cut(s) 1547
Asp700I GAANNNNTTC 2 cut(s) 369, 1767
Asp718I GGTACC 1 cut(s) 1688
AspA2I CCTAGG 1 cut(s) 1343
AspLEI GCGC 4 cut(s) 38, 1971, 2004, 2082
AspS9I GGNCC 4 cut(s) 84, 132, 545, 659
AsuHPI GGTGA 5 cut(s) 139, 378, 1073, 1177, 1991
AsuII TTCGAA 1 cut(s) 1199
AsuNHI GCTAGC 1 cut(s) 977
AvaI CYCGRG 1 cut(s) 102
AvaII GGWCC 2 cut(s) 84, 545
AvrII CCTAGG 1 cut(s) 1343
BaeGI GKGCMC 1 cut(s) 1528
BaeI ACNNNNGTAYC 2 cut(s) 1406, 1439
BalI TGGCCA 1 cut(s) 1296
BamHI GGATCC 1 cut(s) 187
BanI GGYRCC 1 cut(s) 1688
BanII GRGCYC 1 cut(s) 1575
BarI GAAGNNNNNNTAC 2 cut(s) 1613, 1645
BbsI GAAGAC 1 cut(s) 204
Bbv12I GWGCWC 3 cut(s) 519, 1273, 1575
BbvI GCAGC 5 cut(s) 310, 1109, 1351, 1955, 1986
BccI CCATC 6 cut(s) 280, 826, 1090, 1259, 1328, 1475
BceAI ACGGC 2 cut(s) 107, 432
BciT130I CCWGG 2 cut(s) 136, 1977
BclI TGATCA 2 cut(s) 907, 1840
BcoDI GTCTC 4 cut(s) 621, 1824, 1988, 2013
BcuI ACTAGT 1 cut(s) 884
BfmI CTRYAG 2 cut(s) 705, 822
BfuAI ACCTGC 2 cut(s) 1026, 1378
BglI GCCNNNNNGGC 1 cut(s) 119
BlnI CCTAGG 1 cut(s) 1343
BmcAI AGTACT 1 cut(s) 349
Bme1390I CCNGG 2 cut(s) 136, 1977
Bme18I GGWCC 2 cut(s) 84, 545
BmeT110I CYCGRG 1 cut(s) 102
BmgBI CACGTC 1 cut(s) 2007
BmgT120I GGNCC 4 cut(s) 84, 132, 545, 659
BmiI GGNNCC 6 cut(s) 86, 183, 189, 1546, 1690, 1799
BmrFI CCNGG 2 cut(s) 136, 1977
BmrI ACTGGG 1 cut(s) 1687
BmsI GCATC 4 cut(s) 747, 1395, 1432, 1890
BmtI GCTAGC 1 cut(s) 981
BmuI ACTGGG 1 cut(s) 1687
BpiI GAAGAC 1 cut(s) 204
Bpu14I TTCGAA 1 cut(s) 1199
BpuEI CTTGAG 4 cut(s) 748, 813, 1175, 1975
BsaBI GATNNNNATC 1 cut(s) 906
BsaWI WCCGGW 2 cut(s) 190, 1547
BsaXI ACNNNNNCTCC 2 cut(s) 73, 103
Bse118I RCCGGY 3 cut(s) 1547, 1646, 1800
Bse1I ACTGG 1 cut(s) 1693
Bse3DI GCAATG 1 cut(s) 1831
Bse8I GATNNNNATC 1 cut(s) 906
BseAI TCCGGA 1 cut(s) 190
BseBI CCWGG 2 cut(s) 136, 1977
BseGI GGATG 4 cut(s) 539, 679, 1410, 1881
BseJI GATNNNNATC 1 cut(s) 906
BseMI GCAATG 1 cut(s) 1831
BseMII CTCAG 2 cut(s) 299, 2003
BseNI ACTGG 1 cut(s) 1693
BseRI GAGGAG 2 cut(s) 33, 185
BseSI GKGCMC 1 cut(s) 1528
BseXI GCAGC 5 cut(s) 310, 1109, 1351, 1955, 1986
BseYI CCCAGC 3 cut(s) 326, 412, 1793
Bsh1236I CGCG 3 cut(s) 36, 73, 79
Bsh1285I CGRYCG 1 cut(s) 77
BshFI GGCC 8 cut(s) 33, 122, 133, 306, 447, 661, 1252, 1296
BshNI GGYRCC 1 cut(s) 1688
BshTI ACCGGT 1 cut(s) 1547
BsiEI CGRYCG 1 cut(s) 77
BsiHKAI GWGCWC 3 cut(s) 519, 1273, 1575
BsiHKCI CYCGRG 1 cut(s) 102
BsiSI CCGG 6 cut(s) 5, 185, 191, 1548, 1647, 1801
BslFI GGGAC 1 cut(s) 1910
BsmAI GTCTC 4 cut(s) 621, 1824, 1988, 2013
BsmBI CGTCTC 2 cut(s) 1988, 2013
BsmFI GGGAC 1 cut(s) 1910
BsnI GGCC 8 cut(s) 33, 122, 133, 306, 447, 661, 1252, 1296
BsoBI CYCGRG 1 cut(s) 102
Bsp119I TTCGAA 1 cut(s) 1199
Bsp1286I GDGCHC 4 cut(s) 519, 1273, 1528, 1575
Bsp13I TCCGGA 1 cut(s) 190
Bsp143I GATC 8 cut(s) 187, 469, 907, 991, 1232, 1602, 1840, 1936
Bsp19I CCATGG 1 cut(s) 1043
BspANI GGCC 8 cut(s) 33, 122, 133, 306, 447, 661, 1252, 1296
BspCNI CTCAG 2 cut(s) 300, 2002
BspEI TCCGGA 1 cut(s) 190
BspFNI CGCG 3 cut(s) 36, 73, 79
BspLI GGNNCC 6 cut(s) 86, 183, 189, 1546, 1690, 1799
BspMAI CTGCAG 1 cut(s) 709
BspMI ACCTGC 2 cut(s) 1026, 1378
BspOI GCTAGC 1 cut(s) 981
BspPI GGATC 5 cut(s) 182, 195, 1240, 1610, 1944
BspQI GCTCTTC 1 cut(s) 1179
BspT104I TTCGAA 1 cut(s) 1199
BspT107I GGYRCC 1 cut(s) 1688
BsrDI GCAATG 1 cut(s) 1831
BsrFI RCCGGY 3 cut(s) 1547, 1646, 1800
BsrI ACTGG 1 cut(s) 1693
BssAI RCCGGY 3 cut(s) 1547, 1646, 1800
BssMI GATC 8 cut(s) 187, 469, 907, 991, 1232, 1602, 1840, 1936
BssNAI GTATAC 1 cut(s) 1698
BssT1I CCWWGG 4 cut(s) 577, 856, 1043, 1343
Bst1107I GTATAC 1 cut(s) 1698
Bst2UI CCWGG 2 cut(s) 136, 1977
Bst4CI ACNGT 4 cut(s) 97, 937, 1707, 2086
Bst6I CTCTTC 4 cut(s) 162, 333, 979, 1179
BstBI TTCGAA 1 cut(s) 1199
BstC8I GCNNGC 5 cut(s) 49, 53, 696, 979, 1831
BstDEI CTNAG 4 cut(s) 308, 476, 1142, 1989
BstDSI CCRYGG 3 cut(s) 71, 123, 1043
BstEII GGTNACC 2 cut(s) 384, 1038
BstF5I GGATG 4 cut(s) 539, 679, 1410, 1881
BstFNI CGCG 3 cut(s) 36, 73, 79
BstHHI GCGC 4 cut(s) 38, 1971, 2004, 2082
BstKTI GATC 8 cut(s) 190, 472, 910, 994, 1235, 1605, 1843, 1939
BstMAI GTCTC 4 cut(s) 621, 1824, 1988, 2013
BstMBI GATC 8 cut(s) 187, 469, 907, 991, 1232, 1602, 1840, 1936
BstMCI CGRYCG 1 cut(s) 77
BstNI CCWGG 2 cut(s) 136, 1977
BstNSI RCATGY 4 cut(s) 655, 899, 1463, 2030
BstPI GGTNACC 2 cut(s) 384, 1038
BstSCI CCNGG 2 cut(s) 134, 1975
BstSFI CTRYAG 2 cut(s) 705, 822
BstSLI GKGCMC 1 cut(s) 1528
BstUI CGCG 3 cut(s) 36, 73, 79
BstV1I GCAGC 5 cut(s) 310, 1109, 1351, 1955, 1986
BstV2I GAAGAC 1 cut(s) 204
BstX2I RGATCY 1 cut(s) 187
BstXI CCANNNNNNTGG 1 cut(s) 558
BstYI RGATCY 1 cut(s) 187
BstZ17I GTATAC 1 cut(s) 1698
BsuRI GGCC 8 cut(s) 33, 122, 133, 306, 447, 661, 1252, 1296
BtgI CCRYGG 3 cut(s) 71, 123, 1043
BtgZI GCGATG 1 cut(s) 713
BtrI CACGTC 1 cut(s) 2007
BtsCI GGATG 4 cut(s) 539, 679, 1410, 1881
BtsIMutI CAGTG 3 cut(s) 1726, 1843, 2082
BveI ACCTGC 2 cut(s) 1026, 1378
Cac8I GCNNGC 5 cut(s) 49, 53, 696, 979, 1831
CfoI GCGC 4 cut(s) 38, 1971, 2004, 2082
Cfr10I RCCGGY 3 cut(s) 1547, 1646, 1800
Cfr13I GGNCC 4 cut(s) 84, 132, 545, 659
Cfr42I CCGCGG 1 cut(s) 74
CseI GACGC 2 cut(s) 1547, 2005
CsiI ACCWGGT 1 cut(s) 1975
Csp6I GTAC 6 cut(s) 23, 348, 537, 605, 1367, 1689
CspAI ACCGGT 1 cut(s) 1547
CspCI CAANNNNNGTGG 2 cut(s) 1874, 1909
CviQI GTAC 6 cut(s) 23, 348, 537, 605, 1367, 1689
DdeI CTNAG 4 cut(s) 308, 476, 1142, 1989
DpnI GATC 8 cut(s) 189, 471, 909, 993, 1234, 1604, 1842, 1938
DpnII GATC 8 cut(s) 187, 469, 907, 991, 1232, 1602, 1840, 1936
EaeI YGGCCR 2 cut(s) 445, 1294
Eam1104I CTCTTC 4 cut(s) 162, 333, 979, 1179
EarI CTCTTC 4 cut(s) 162, 333, 979, 1179
Ecl136II GAGCTC 1 cut(s) 1573
Eco130I CCWWGG 4 cut(s) 577, 856, 1043, 1343
Eco24I GRGCYC 1 cut(s) 1575
Eco32I GATATC 1 cut(s) 216
Eco47I GGWCC 2 cut(s) 84, 545
Eco53kI GAGCTC 1 cut(s) 1573
Eco57I CTGAAG 2 cut(s) 672, 1819
Eco88I CYCGRG 1 cut(s) 102
Eco91I GGTNACC 2 cut(s) 384, 1038
EcoICRI GAGCTC 1 cut(s) 1573
EcoO109I RGGNCCY 2 cut(s) 84, 659
EcoO65I GGTNACC 2 cut(s) 384, 1038
EcoRI GAATTC 1 cut(s) 682
EcoRII CCWGG 2 cut(s) 134, 1975
EcoRV GATATC 1 cut(s) 216
EcoT14I CCWWGG 4 cut(s) 577, 856, 1043, 1343
EcoT38I GRGCYC 1 cut(s) 1575
ErhI CCWWGG 4 cut(s) 577, 856, 1043, 1343
Esp3I CGTCTC 2 cut(s) 1988, 2013
FalI AAGNNNNNCTT 2 cut(s) 1932, 1964
FaqI GGGAC 1 cut(s) 1910
FauI CCCGC 1 cut(s) 1822
FauNDI CATATG 1 cut(s) 553
FbaI TGATCA 2 cut(s) 907, 1840
FblI GTMKAC 1 cut(s) 1697
FokI GGATG 4 cut(s) 546, 686, 1417, 1868
FriOI GRGCYC 1 cut(s) 1575
FspI TGCGCA 1 cut(s) 2003
GlaI GCGC 4 cut(s) 37, 1970, 2003, 2081
GsaI CCCAGC 3 cut(s) 330, 416, 1797
HaeIII GGCC 8 cut(s) 33, 122, 133, 306, 447, 661, 1252, 1296
HapII CCGG 6 cut(s) 5, 185, 191, 1548, 1647, 1801
HgaI GACGC 2 cut(s) 1547, 2005
HhaI GCGC 4 cut(s) 38, 1971, 2004, 2082
Hin6I GCGC 4 cut(s) 36, 1969, 2002, 2080
HinP1I GCGC 4 cut(s) 36, 1969, 2002, 2080
HincII GTYRAC 1 cut(s) 853
HindII GTYRAC 1 cut(s) 853
HinfI GANTC 6 cut(s) 227, 233, 734, 998, 1724, 2050
HpaII CCGG 6 cut(s) 5, 185, 191, 1548, 1647, 1801
HphI GGTGA 5 cut(s) 139, 378, 1073, 1177, 1991
Hpy166II GTNNAC 4 cut(s) 568, 853, 1168, 1698
Hpy188I TCNGA 4 cut(s) 61, 232, 238, 1872
Hpy8I GTNNAC 4 cut(s) 568, 853, 1168, 1698
Hpy99I CGWCG 2 cut(s) 101, 453
HpyAV CCTTC 6 cut(s) 1345, 1591, 1744, 1757, 1871, 2026
HpyCH4III ACNGT 4 cut(s) 97, 937, 1707, 2086
HpyCH4IV ACGT 3 cut(s) 913, 1891, 2006
HpyCH4V TGCA 6 cut(s) 707, 760, 778, 1086, 1478, 1591
HpyF3I CTNAG 4 cut(s) 308, 476, 1142, 1989
HpySE526I ACGT 3 cut(s) 913, 1891, 2006
HspAI GCGC 4 cut(s) 36, 1969, 2002, 2080
Kpn2I TCCGGA 1 cut(s) 190
KpnI GGTACC 1 cut(s) 1692
Ksp22I TGATCA 2 cut(s) 907, 1840
KspI CCGCGG 1 cut(s) 74
Kzo9I GATC 8 cut(s) 187, 469, 907, 991, 1232, 1602, 1840, 1936
LguI GCTCTTC 1 cut(s) 1179
LmnI GCTCC 4 cut(s) 7, 181, 1797, 1852
Lsp1109I GCAGC 5 cut(s) 310, 1109, 1351, 1955, 1986
LweI GCATC 4 cut(s) 747, 1395, 1432, 1890
MabI ACCWGGT 1 cut(s) 1975
MaeII ACGT 3 cut(s) 913, 1891, 2006
MaeIII GTNAC 6 cut(s) 384, 454, 1038, 1774, 1979, 2072
MalI GATC 8 cut(s) 189, 471, 909, 993, 1234, 1604, 1842, 1938
MboI GATC 8 cut(s) 187, 469, 907, 991, 1232, 1602, 1840, 1936
MflI RGATCY 1 cut(s) 187
MhlI GDGCHC 4 cut(s) 519, 1273, 1528, 1575
MlsI TGGCCA 1 cut(s) 1296
MluNI TGGCCA 1 cut(s) 1296
MlyI GAGTC 2 cut(s) 221, 743
MmeI TCCRAC 1 cut(s) 84
Mox20I TGGCCA 1 cut(s) 1296
MroI TCCGGA 1 cut(s) 190
MroXI GAANNNNTTC 2 cut(s) 369, 1767
MscI TGGCCA 1 cut(s) 1296
MseI TTAA 2 cut(s) 507, 1205
MslI CAYNNNNRTG 4 cut(s) 556, 783, 1431, 2027
Msp20I TGGCCA 1 cut(s) 1296
MspA1I CMGCKG 1 cut(s) 73
MspI CCGG 6 cut(s) 5, 185, 191, 1548, 1647, 1801
MspR9I CCNGG 2 cut(s) 136, 1977
MvaI CCWGG 2 cut(s) 136, 1977
MvnI CGCG 3 cut(s) 36, 73, 79
NcoI CCATGG 1 cut(s) 1043
NdeI CATATG 1 cut(s) 553
NdeII GATC 8 cut(s) 187, 469, 907, 991, 1232, 1602, 1840, 1936
NheI GCTAGC 1 cut(s) 977
NlaIV GGNNCC 6 cut(s) 86, 183, 189, 1546, 1690, 1799
NmeAIII GCCGAG 2 cut(s) 53, 142
NmuCI GTSAC 3 cut(s) 384, 1774, 1979
NsbI TGCGCA 1 cut(s) 2003
NspI RCATGY 4 cut(s) 655, 899, 1463, 2030
NspV TTCGAA 1 cut(s) 1199
OliI CACNNNNGTG 1 cut(s) 2027
PciI ACATGT 2 cut(s) 1459, 2026
PciSI GCTCTTC 1 cut(s) 1179
PcsI WCGNNNNNNNCGW 1 cut(s) 2003
PdmI GAANNNNTTC 2 cut(s) 369, 1767
PfeI GAWTC 4 cut(s) 233, 998, 1724, 2050
PflMI CCANNNNNTGG 1 cut(s) 1380
PinAI ACCGGT 1 cut(s) 1547
PleI GAGTC 2 cut(s) 221, 742
PpsI GAGTC 2 cut(s) 221, 742
PpuMI RGGWCCY 1 cut(s) 84
PscI ACATGT 2 cut(s) 1459, 2026
PsiI TTATAA 2 cut(s) 612, 1493
Psp124BI GAGCTC 1 cut(s) 1575
Psp5II RGGWCCY 1 cut(s) 84
Psp6I CCWGG 2 cut(s) 134, 1975
PspEI GGTNACC 2 cut(s) 384, 1038
PspFI CCCAGC 3 cut(s) 326, 412, 1793
PspGI CCWGG 2 cut(s) 134, 1975
PspN4I GGNNCC 6 cut(s) 86, 183, 189, 1546, 1690, 1799
PspPI GGNCC 4 cut(s) 84, 132, 545, 659
PspPPI RGGWCCY 1 cut(s) 84
PsrI GAACNNNNNNTAC 2 cut(s) 1537, 1569
PstI CTGCAG 1 cut(s) 709
PsuI RGATCY 1 cut(s) 187
RsaI GTAC 6 cut(s) 24, 349, 538, 606, 1368, 1690
RsaNI GTAC 6 cut(s) 23, 348, 537, 605, 1367, 1689
RseI CAYNNNNRTG 4 cut(s) 556, 783, 1431, 2027
SacI GAGCTC 1 cut(s) 1575
SacII CCGCGG 1 cut(s) 74
SapI GCTCTTC 1 cut(s) 1179
SaqAI TTAA 2 cut(s) 507, 1205
Sau3AI GATC 8 cut(s) 187, 469, 907, 991, 1232, 1602, 1840, 1936
Sau96I GGNCC 4 cut(s) 84, 132, 545, 659
ScaI AGTACT 1 cut(s) 349
SchI GAGTC 2 cut(s) 221, 743
ScrFI CCNGG 2 cut(s) 136, 1977
SduI GDGCHC 4 cut(s) 519, 1273, 1528, 1575
SexAI ACCWGGT 1 cut(s) 1975
SfaNI GCATC 4 cut(s) 747, 1395, 1432, 1890
SfcI CTRYAG 2 cut(s) 705, 822
Sfr303I CCGCGG 1 cut(s) 74
SfuI TTCGAA 1 cut(s) 1199
SgrBI CCGCGG 1 cut(s) 74
SinI GGWCC 2 cut(s) 84, 545
SmiMI CAYNNNNRTG 4 cut(s) 556, 783, 1431, 2027
SmlI CTYRAG 4 cut(s) 763, 828, 1154, 1954
SmoI CTYRAG 4 cut(s) 763, 828, 1154, 1954
SpeI ACTAGT 1 cut(s) 884
SspI AATATT 1 cut(s) 1318
SstI GAGCTC 1 cut(s) 1575
StyD4I CCNGG 2 cut(s) 134, 1975
StyI CCWWGG 4 cut(s) 577, 856, 1043, 1343
TaaI ACNGT 4 cut(s) 97, 937, 1707, 2086
TaiI ACGT 3 cut(s) 916, 1894, 2009
TaqI TCGA 5 cut(s) 264, 513, 732, 1199, 1919
TatI WGTACW 3 cut(s) 347, 536, 604
TauI GCSGC 5 cut(s) 36, 73, 113, 116, 1928
TfiI GAWTC 4 cut(s) 233, 998, 1724, 2050
Tru1I TTAA 2 cut(s) 507, 1205
Tru9I TTAA 2 cut(s) 507, 1205
TscAI CASTG 3 cut(s) 1726, 1843, 2089
TseFI GTSAC 3 cut(s) 384, 1774, 1979
TseI GCWGC 5 cut(s) 323, 1122, 1339, 1943, 1999
Tsp45I GTSAC 3 cut(s) 384, 1774, 1979
TspDTI ATGAA 5 cut(s) 944, 1187, 1197, 1427, 1760
TspGWI ACGGA 1 cut(s) 208
TspRI CASTG 3 cut(s) 1726, 1843, 2089
Van91I CCANNNNNTGG 1 cut(s) 1380
VpaK11BI GGWCC 2 cut(s) 84, 545
XapI RAATTY 4 cut(s) 509, 682, 799, 1175
XbaI TCTAGA 1 cut(s) 748
XceI RCATGY 4 cut(s) 655, 899, 1463, 2030
XcmI CCANNNNNNNNNTGG 1 cut(s) 142
XmaJI CCTAGG 1 cut(s) 1343
XmiI GTMKAC 1 cut(s) 1697
XmnI GAANNNNTTC 2 cut(s) 369, 1767
ZrmI AGTACT 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.