Rh5CG574400

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0011667
Physical Location & Seq
Reverse (-)
81407740 .. 81414125
6386 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG574400.1

Sequence Viewer

Length: 1458 bp
ATGGTTATGGACATGCTAGGACCTAGTTTATGGGATGTATGGAATACTTCAGGGCAAGCGATGTCTGCAGAAATGGTAGCTTGTATAGCTGTTGAGTCTTTATCGATTCTGGAGAAGATGCACTCTAGAGGGTATGTGCATGGAGATGTAAAGCCAGAGAACTTTTTGCTTGGTCAGCCATCTACCGCTCAAGAGAAGAAATTGTATCTTGTTGACCTCGGATTAGCAACAAAGTGGAAAGACAGCTCAAGTGGGCTTCATGTTGAATATGATCAACGTCCTGATATGTTTAGAGGAACTGTTCGATATGCTAGTGTACATGCTCACTTGGGAAGAACTGCTAGTAGACGAGATGACCTCGAATCACTTGCTTATACACTTATTTTTCTCCATCGAGGAAGGTTGCCATGGCAGGGGTATCAGGGTGATAACAAATCTTTCCTAGTTTGTAAAAAGAAGATGGCAACATCACCTGAAATGCTGTGCTGTTTCTGCCCTGCAGCTCTAAGGCAATTTCTTGAGATCGTGGTGAACATGAAATTTGATGAAGAGCCTAACTATTCAAAATTAATATCACTGTTTGAGGGATTGATAGGAACAAATCCTGCTGTTAGACCATTAAAAATTGATGGTGCTCAGAAGATTATTAGCCAGGTTGGCCAGAAACGGGGTAGATTGAATATTGAGGAAGATGATGACGGGCAGCCAAGGAAGAAGGTTCGGCTAGGAGTACCTGCTACACAATGGATTTCAATTTACAATGCTCGGATGCCAATGAAACAAAGGTATCATTACAACGTCGCTGATGCAAGGTTGGCACAACATGTGGAGAGAGGAATTGCAGATGGTCTGCTTATAAGTTGCGTGTCTTCATGTTCTAATCTCTGGGCACTTATTATGGATGCTGGAACTGGTTTTTCAAACCAAGTTTATGAGCTTTCTCCCTTCTTCTTGCACAAGGAATGGATAATGGAACAATGGGAAAAGAATTATTACATTAGTTCTATTGCTGGTGCTAACAATGGGAGTTCCCTTGTGGTGATGTCCAAAGGAACCCAGTATACTCAACAGTCTTACAAAGTGAGCGATTCTTTCCCCTTCAAGTGGATAAACAAAAAGTGGAGGGAAGGTTTTCATGTAACTTCAATGGCAACTGCTGGGAGCCGGTGGGGTGTTGTAATGTCTCGGAATGCTGGCTTCAGTGATCAGGTTGTGGAGCTTGATTTTCTCTATCCAAGTGAAGGCATCCACAGGCGTTGGGATAATGGTTTCCGGATAACATCAACAGCTGCAACCTGGGATCAAGCGGCTCTTATCTTGAGTGTTCCCAAGCGCAAACCTGGTGATGAAACACAGGAGACCCTGCGTACATCACAATTTCCTAGCACACATGTCAAGGAAAAATGGGCAAAGAATCTGTATCTTGCTTGTTTGTGCTATGGACGTACCGTATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04392 map04011 map04392
Pfam Domains
Protein Families
Pkinase

Protein Analysis

485

Amino Acids

54.92

Weight (kDa)

8.94

Isoelectric Point (pI)

44.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 1 - 154 1.9e-12 Protein kinase domain
Pkinase_fungal PF17667 34 - 129 6e-07 Fungal protein kinase
DUF7477 PF24289 218 - 485 7.1e-163 Domain of unknown function (DUF7477)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011667)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13670
fragaria_vesca FvH4_3g44690
malus_domestica MD03G1011500.v1.1 MD11G1014200.v1.1
prunus_persica Prupe.6G011900_v2.0.a1 Prupe.6G011900_v2.0.a1 Prupe.6G011900_v2.0.a1
pyrus_communis pycom03g01040 pycom11g00960
rosa_chinensis RchiOBHm_Chr5g0081361
rosa_laevigata RLG00000036927
rosa_multiflora Rmu_sc0007768.1_g000010 Rmu_sc0009187.1_g000017
rosa_roxburghii Rroxscaffold_1G00002180
rosa_rugosa Rorug05G0476000
rosa_samantha Rh5AG527400 Rh5BG551600 Rh5CG574400
rosa_wichuraiana Rw5G049050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 857
Acc36I ACCTGC 1 cut(s) 742
AccBSI CCGCTC 1 cut(s) 188
AccI GTMKAC 2 cut(s) 346, 1061
AccIII TCCGGA 1 cut(s) 1272
AciI CCGC 2 cut(s) 186, 1307
AclWI GGATC 1 cut(s) 1308
AcoI YGGCCR 1 cut(s) 658
AcsI RAATTY 1 cut(s) 539
AcuI CTGAAG 2 cut(s) 33, 1183
AfaI GTAC 4 cut(s) 318, 732, 1369, 1447
AfiI CCNNNNNNNGG 4 cut(s) 413, 667, 1104, 1241
AflIII ACRYGT 2 cut(s) 823, 1390
AgsI TTSAA 7 cut(s) 266, 564, 679, 753, 921, 1102, 1146
AjnI CCWGG 3 cut(s) 651, 1295, 1339
AluBI AGCT 7 cut(s) 80, 89, 246, 503, 937, 1219, 1289
AluI AGCT 7 cut(s) 80, 89, 246, 503, 937, 1219, 1289
Alw21I GWGCWC 1 cut(s) 637
Alw26I GTCTC 2 cut(s) 1188, 1352
AlwI GGATC 1 cut(s) 1308
Aor13HI TCCGGA 1 cut(s) 1272
AoxI GGCC 1 cut(s) 658
ApeKI GCWGC 3 cut(s) 500, 703, 1289
ApoI RAATTY 1 cut(s) 539
AseI ATTAAT 1 cut(s) 569
Asp700I GAANNNNTTC 1 cut(s) 1131
AspLEI GCGC 1 cut(s) 1335
AspS9I GGNCC 1 cut(s) 20
AsuHPI GGTGA 5 cut(s) 437, 462, 541, 1051, 1355
AvaII GGWCC 1 cut(s) 20
BaeGI GKGCMC 1 cut(s) 892
BaeI ACNNNNGTAYC 2 cut(s) 770, 803
BalI TGGCCA 1 cut(s) 660
BbsI GAAGAC 1 cut(s) 861
Bbv12I GWGCWC 1 cut(s) 637
BbvI GCAGC 3 cut(s) 512, 715, 1276
BccI CCATC 5 cut(s) 187, 399, 454, 623, 839
BcgI CGANNNNNNTGC 2 cut(s) 350, 384
BciT130I CCWGG 3 cut(s) 653, 1297, 1341
BclI TGATCA 2 cut(s) 271, 1204
BcoDI GTCTC 2 cut(s) 1188, 1352
BfaI CTAG 8 cut(s) 17, 24, 126, 312, 342, 443, 725, 1383
BfmI CTRYAG 2 cut(s) 66, 498
BfuAI ACCTGC 1 cut(s) 742
BglI GCCNNNNNGGC 1 cut(s) 657
BisI GCNGC 4 cut(s) 501, 704, 1290, 1308
BlsI GCNGC 4 cut(s) 502, 705, 1291, 1309
Bme1390I CCNGG 3 cut(s) 653, 1297, 1341
Bme18I GGWCC 1 cut(s) 20
BmgT120I GGNCC 1 cut(s) 20
BmiI GGNNCC 2 cut(s) 1054, 1163
BmrFI CCNGG 3 cut(s) 653, 1297, 1341
BmrI ACTGGG 1 cut(s) 1051
BmsI GCATC 5 cut(s) 108, 759, 796, 892, 1254
BmuI ACTGGG 1 cut(s) 1051
BpiI GAAGAC 1 cut(s) 861
BplI GAGNNNNNCTC 2 cut(s) 342, 374
BpmI CTGGAG 1 cut(s) 131
BpuEI CTTGAG 4 cut(s) 174, 232, 539, 1339
Bsa29I ATCGAT 1 cut(s) 104
BsaI GGTCTC 1 cut(s) 1352
BsaJI CCNNGG 4 cut(s) 217, 407, 707, 1296
BsaWI WCCGGW 1 cut(s) 1272
BsaXI ACNNNNNCTCC 2 cut(s) 1114, 1144
Bsc4I CCNNNNNNNGG 4 cut(s) 413, 667, 1104, 1241
Bse118I RCCGGY 1 cut(s) 1164
Bse1I ACTGG 2 cut(s) 916, 1057
BseAI TCCGGA 1 cut(s) 1272
BseBI CCWGG 3 cut(s) 653, 1297, 1341
BseCI ATCGAT 1 cut(s) 104
BseDI CCNNGG 4 cut(s) 217, 407, 707, 1296
BseGI GGATG 4 cut(s) 40, 774, 907, 1245
BseLI CCNNNNNNNGG 4 cut(s) 413, 667, 1104, 1241
BseMII CTCAG 1 cut(s) 650
BseNI ACTGG 2 cut(s) 916, 1057
BseSI GKGCMC 1 cut(s) 892
BseXI GCAGC 3 cut(s) 512, 715, 1276
BseYI CCCAGC 1 cut(s) 1157
BshFI GGCC 1 cut(s) 660
BshVI ATCGAT 1 cut(s) 104
BsiHKAI GWGCWC 1 cut(s) 637
BsiSI CCGG 2 cut(s) 1165, 1273
BslI CCNNNNNNNGG 4 cut(s) 413, 667, 1104, 1241
BsmAI GTCTC 2 cut(s) 1188, 1352
BsmI GAATGC 1 cut(s) 1195
BsnI GGCC 1 cut(s) 660
Bso31I GGTCTC 1 cut(s) 1352
Bsp1286I GDGCHC 2 cut(s) 637, 892
Bsp13I TCCGGA 1 cut(s) 1272
Bsp1407I TGTACA 1 cut(s) 316
Bsp143I GATC 4 cut(s) 271, 522, 1204, 1300
Bsp19I CCATGG 1 cut(s) 407
BspACI CCGC 2 cut(s) 186, 1307
BspANI GGCC 1 cut(s) 660
BspCNI CTCAG 1 cut(s) 649
BspDI ATCGAT 1 cut(s) 104
BspEI TCCGGA 1 cut(s) 1272
BspLI GGNNCC 2 cut(s) 1054, 1163
BspMAI CTGCAG 2 cut(s) 70, 502
BspMI ACCTGC 1 cut(s) 742
BspPI GGATC 1 cut(s) 1308
BspQI GCTCTTC 1 cut(s) 543
BspTNI GGTCTC 1 cut(s) 1352
BsrBI CCGCTC 1 cut(s) 188
BsrFI RCCGGY 1 cut(s) 1164
BsrGI TGTACA 1 cut(s) 316
BsrI ACTGG 2 cut(s) 916, 1057
BssAI RCCGGY 1 cut(s) 1164
BssECI CCNNGG 4 cut(s) 217, 407, 707, 1296
BssMI GATC 4 cut(s) 271, 522, 1204, 1300
BssNAI GTATAC 1 cut(s) 1062
BssT1I CCWWGG 2 cut(s) 407, 707
Bst1107I GTATAC 1 cut(s) 1062
Bst2UI CCWGG 3 cut(s) 653, 1297, 1341
Bst4CI ACNGT 4 cut(s) 301, 579, 1071, 1450
Bst6I CTCTTC 1 cut(s) 543
BstAUI TGTACA 1 cut(s) 316
BstC8I GCNNGC 2 cut(s) 57, 1195
BstDEI CTNAG 2 cut(s) 506, 636
BstDSI CCRYGG 1 cut(s) 407
BstF5I GGATG 4 cut(s) 40, 774, 907, 1245
BstHHI GCGC 1 cut(s) 1335
BstKTI GATC 4 cut(s) 274, 525, 1207, 1303
BstMAI GTCTC 2 cut(s) 1188, 1352
BstMBI GATC 4 cut(s) 271, 522, 1204, 1300
BstMWI GCNNNNNNNGC 6 cut(s) 65, 86, 175, 492, 657, 815
BstNI CCWGG 3 cut(s) 653, 1297, 1341
BstNSI RCATGY 4 cut(s) 16, 323, 827, 1394
BstSCI CCNGG 3 cut(s) 651, 1295, 1339
BstSFI CTRYAG 2 cut(s) 66, 498
BstSLI GKGCMC 1 cut(s) 892
BstV1I GCAGC 3 cut(s) 512, 715, 1276
BstV2I GAAGAC 1 cut(s) 861
BstZ17I GTATAC 1 cut(s) 1062
Bsu15I ATCGAT 1 cut(s) 104
BsuRI GGCC 1 cut(s) 660
BsuTUI ATCGAT 1 cut(s) 104
BtgI CCRYGG 1 cut(s) 407
BtgZI GCGATG 1 cut(s) 74
BtsCI GGATG 4 cut(s) 40, 774, 907, 1245
BtsIMutI CAGTG 2 cut(s) 575, 1207
BveI ACCTGC 1 cut(s) 742
Cac8I GCNNGC 2 cut(s) 57, 1195
CfoI GCGC 1 cut(s) 1335
Cfr10I RCCGGY 1 cut(s) 1164
Cfr13I GGNCC 1 cut(s) 20
ClaI ATCGAT 1 cut(s) 104
CsiI ACCWGGT 1 cut(s) 1339
Csp6I GTAC 4 cut(s) 317, 731, 1368, 1446
CspCI CAANNNNNGTGG 2 cut(s) 1238, 1273
CviQI GTAC 4 cut(s) 317, 731, 1368, 1446
DdeI CTNAG 2 cut(s) 506, 636
DpnI GATC 4 cut(s) 273, 524, 1206, 1302
DpnII GATC 4 cut(s) 271, 522, 1204, 1300
EaeI YGGCCR 1 cut(s) 658
Eam1104I CTCTTC 1 cut(s) 543
EarI CTCTTC 1 cut(s) 543
Eco130I CCWWGG 2 cut(s) 407, 707
Eco31I GGTCTC 1 cut(s) 1352
Eco47I GGWCC 1 cut(s) 20
Eco57I CTGAAG 2 cut(s) 33, 1183
EcoO109I RGGNCCY 1 cut(s) 20
EcoRII CCWGG 3 cut(s) 651, 1295, 1339
EcoT14I CCWWGG 2 cut(s) 407, 707
ErhI CCWWGG 2 cut(s) 407, 707
FalI AAGNNNNNCTT 2 cut(s) 1296, 1328
FbaI TGATCA 2 cut(s) 271, 1204
FblI GTMKAC 2 cut(s) 346, 1061
Fnu4HI GCNGC 4 cut(s) 501, 704, 1290, 1308
FokI GGATG 4 cut(s) 47, 781, 914, 1232
Fsp4HI GCNGC 4 cut(s) 501, 704, 1290, 1308
FspBI CTAG 8 cut(s) 17, 24, 126, 312, 342, 443, 725, 1383
GlaI GCGC 1 cut(s) 1334
GluI GCNGC 4 cut(s) 501, 704, 1290, 1308
GsaI CCCAGC 1 cut(s) 1161
GsuI CTGGAG 1 cut(s) 131
HaeIII GGCC 1 cut(s) 660
HapII CCGG 2 cut(s) 1165, 1273
HhaI GCGC 1 cut(s) 1335
Hin6I GCGC 1 cut(s) 1333
HinP1I GCGC 1 cut(s) 1333
HincII GTYRAC 1 cut(s) 214
HindII GTYRAC 1 cut(s) 214
HinfI GANTC 5 cut(s) 95, 106, 362, 1088, 1414
HpaII CCGG 2 cut(s) 1165, 1273
HphI GGTGA 5 cut(s) 437, 462, 541, 1051, 1355
Hpy166II GTNNAC 5 cut(s) 214, 317, 347, 532, 1062
Hpy188I TCNGA 4 cut(s) 221, 639, 768, 1188
Hpy188III TCNNGA 7 cut(s) 110, 126, 191, 281, 518, 1273, 1318
Hpy8I GTNNAC 5 cut(s) 214, 317, 347, 532, 1062
Hpy99I CGWCG 1 cut(s) 803
HpyAV CCTTC 6 cut(s) 393, 709, 955, 1108, 1121, 1235
HpyCH4III ACNGT 4 cut(s) 301, 579, 1071, 1450
HpyCH4IV ACGT 3 cut(s) 277, 798, 1444
HpyCH4V TGCA 8 cut(s) 68, 121, 139, 500, 809, 842, 955, 1292
HpyF10VI GCNNNNNNNGC 6 cut(s) 65, 86, 175, 492, 657, 815
HpyF3I CTNAG 2 cut(s) 506, 636
HpySE526I ACGT 3 cut(s) 277, 798, 1444
HspAI GCGC 1 cut(s) 1333
Kpn2I TCCGGA 1 cut(s) 1272
Ksp22I TGATCA 2 cut(s) 271, 1204
Kzo9I GATC 4 cut(s) 271, 522, 1204, 1300
LguI GCTCTTC 1 cut(s) 543
LmnI GCTCC 2 cut(s) 1161, 1216
Lsp1109I GCAGC 3 cut(s) 512, 715, 1276
LweI GCATC 5 cut(s) 108, 759, 796, 892, 1254
MabI ACCWGGT 1 cut(s) 1339
MaeI CTAG 8 cut(s) 17, 24, 126, 312, 342, 443, 725, 1383
MaeII ACGT 3 cut(s) 277, 798, 1444
MaeIII GTNAC 1 cut(s) 1138
MalI GATC 4 cut(s) 273, 524, 1206, 1302
MbiI CCGCTC 1 cut(s) 188
MboI GATC 4 cut(s) 271, 522, 1204, 1300
MhlI GDGCHC 2 cut(s) 637, 892
MlsI TGGCCA 1 cut(s) 660
MluCI AATT 9 cut(s) 200, 512, 539, 566, 624, 753, 837, 988, 1376
MluNI TGGCCA 1 cut(s) 660
MlyI GAGTC 1 cut(s) 104
MnlI CCTC 9 cut(s) 122, 227, 287, 368, 389, 577, 679, 827, 1116
Mox20I TGGCCA 1 cut(s) 660
MroI TCCGGA 1 cut(s) 1272
MroXI GAANNNNTTC 1 cut(s) 1131
MscI TGGCCA 1 cut(s) 660
MseI TTAA 3 cut(s) 569, 620, 1456
MslI CAYNNNNRTG 1 cut(s) 144
Msp20I TGGCCA 1 cut(s) 660
MspA1I CMGCKG 1 cut(s) 1289
MspI CCGG 2 cut(s) 1165, 1273
MspR9I CCNGG 3 cut(s) 653, 1297, 1341
Mva1269I GAATGC 1 cut(s) 1195
MvaI CCWGG 3 cut(s) 653, 1297, 1341
MwoI GCNNNNNNNGC 6 cut(s) 65, 86, 175, 492, 657, 815
NcoI CCATGG 1 cut(s) 407
NdeII GATC 4 cut(s) 271, 522, 1204, 1300
NlaIV GGNNCC 2 cut(s) 1054, 1163
NspI RCATGY 4 cut(s) 16, 323, 827, 1394
PciI ACATGT 2 cut(s) 823, 1390
PciSI GCTCTTC 1 cut(s) 543
PctI GAATGC 1 cut(s) 1195
PdmI GAANNNNTTC 1 cut(s) 1131
PfeI GAWTC 4 cut(s) 106, 362, 1088, 1414
PkrI GCNGC 4 cut(s) 502, 705, 1291, 1309
PleI GAGTC 1 cut(s) 103
PpsI GAGTC 1 cut(s) 103
PpuMI RGGWCCY 1 cut(s) 20
PscI ACATGT 2 cut(s) 823, 1390
PshBI ATTAAT 1 cut(s) 569
PsiI TTATAA 1 cut(s) 857
Psp5II RGGWCCY 1 cut(s) 20
Psp6I CCWGG 3 cut(s) 651, 1295, 1339
PspFI CCCAGC 1 cut(s) 1157
PspGI CCWGG 3 cut(s) 651, 1295, 1339
PspN4I GGNNCC 2 cut(s) 1054, 1163
PspPI GGNCC 1 cut(s) 20
PspPPI RGGWCCY 1 cut(s) 20
PsrI GAACNNNNNNTAC 2 cut(s) 1045, 1077
PstI CTGCAG 2 cut(s) 70, 502
PvuII CAGCTG 1 cut(s) 1289
RsaI GTAC 4 cut(s) 318, 732, 1369, 1447
RsaNI GTAC 4 cut(s) 317, 731, 1368, 1446
RseI CAYNNNNRTG 1 cut(s) 144
SapI GCTCTTC 1 cut(s) 543
SaqAI TTAA 3 cut(s) 569, 620, 1456
SatI GCNGC 4 cut(s) 501, 704, 1290, 1308
Sau3AI GATC 4 cut(s) 271, 522, 1204, 1300
Sau96I GGNCC 1 cut(s) 20
SchI GAGTC 1 cut(s) 104
ScrFI CCNGG 3 cut(s) 653, 1297, 1341
SduI GDGCHC 2 cut(s) 637, 892
SexAI ACCWGGT 1 cut(s) 1339
SfaNI GCATC 5 cut(s) 108, 759, 796, 892, 1254
SfcI CTRYAG 2 cut(s) 66, 498
SinI GGWCC 1 cut(s) 20
SmiMI CAYNNNNRTG 1 cut(s) 144
SmlI CTYRAG 4 cut(s) 189, 247, 518, 1318
SmoI CTYRAG 4 cut(s) 189, 247, 518, 1318
Sse9I AATT 9 cut(s) 200, 512, 539, 566, 624, 753, 837, 988, 1376
SsiI CCGC 2 cut(s) 186, 1307
SspI AATATT 1 cut(s) 682
SspMI CTAG 8 cut(s) 17, 24, 126, 312, 342, 443, 725, 1383
StyD4I CCNGG 3 cut(s) 651, 1295, 1339
StyI CCWWGG 2 cut(s) 407, 707
TaaI ACNGT 4 cut(s) 301, 579, 1071, 1450
TaiI ACGT 3 cut(s) 280, 801, 1447
TaqI TCGA 4 cut(s) 104, 304, 360, 394
TasI AATT 9 cut(s) 200, 512, 539, 566, 624, 753, 837, 988, 1376
TatI WGTACW 1 cut(s) 316
TauI GCSGC 1 cut(s) 1310
TfiI GAWTC 4 cut(s) 106, 362, 1088, 1414
Tru1I TTAA 3 cut(s) 569, 620, 1456
Tru9I TTAA 3 cut(s) 569, 620, 1456
TscAI CASTG 2 cut(s) 582, 1207
TseI GCWGC 3 cut(s) 500, 703, 1289
TspDTI ATGAA 7 cut(s) 248, 551, 561, 791, 861, 1124, 1362
TspRI CASTG 2 cut(s) 582, 1207
VpaK11BI GGWCC 1 cut(s) 20
VspI ATTAAT 1 cut(s) 569
XapI RAATTY 1 cut(s) 539
XbaI TCTAGA 1 cut(s) 125
XceI RCATGY 4 cut(s) 16, 323, 827, 1394
XmiI GTMKAC 2 cut(s) 346, 1061
XmnI GAANNNNTTC 1 cut(s) 1131
XspI CTAG 8 cut(s) 17, 24, 126, 312, 342, 443, 725, 1383
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.