MD11G1014200.v1.1

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0011667
Physical Location & Seq
Forward (+)
1141862 .. 1147108
5247 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1014200.v1.1.491

Sequence Viewer

Length: 2100 bp
ATGCCGGAGCTTCGCAGAGGAGTCCGCCGAGGCCGTGCTAGGGTAGCGAGCAAGCCCTCCGACCCACCGCCGCGGTCGCGGAGGACGCGTGCCACCGTCGCCAGAGAAGCCGCCGCCGAGGCCGTGGTGAGGCCCAGGACGAGGTTGGCTGTGAGGAAATTGAAAGAAGAGGAGAAGCAGGAGCCGGTGCCGGTGCTGGAGGAAGACCCTGTGATTGTGATATCGGTGAAAGACTCTGATTCGGAAGGCAGTAAGGGAGAAGAAAAAGTCGAGGAAGATAAGAAAGCTGTGATGGCTGACGATAGTGGTGGCCTGAGTGCTAATAAGGCTGCTGGGCAAGAAGAAGAGGGCAGTACTGCGCCTTTCCCTGAAAAGGTTCAAGTAGGAGGGTCACCACTCTATAAGGTAGAAAGGAAGCTGGGAAAAGGTGGCTTTGGTCAAGTGTTTGTTGGCCGTCGTGTTACGGGTGGAGTTGATCGTTTAAGTGGTGCTGGTGCTATTGAGGTAGCACTTAAATTCGAGCACAGAAACAGCAAAGGCTGTACTTATGGTCCTCCATATGAGTGGCAAGTTTACAACACGCTTGGTGGTAGTCATGGTGTGCCGAAAGTACATTATAAAGGAAAGCAAGGAGACTATTATGTTATGGTTATGGACATGCTAGGGCCTAGTTTATGGGATGTATGGAATTCTTCAGGGCAAATAATGTCTGCAGAAATGGTGGCTTGCATAGCTGTCGAGTCCTTATCAATTCTAGAGAAGATGCACTCAAGAGGCTATGTGCATGGAGATGTAAAGCCCGAAAACTTTTTACTTGGTCAGCCATCTACAGCTCAGGAGAAGAAGTTGTTTCTTGTTGACCTTGGATTAGCGACAAAGTGGAAAGACACTAGTGGGGGGCATGTTGATTATGATCAACGTCCTGATATGTTTAGAGGAACTGTGCGATATGCTAGTGTTCATGCTCATCTGGGAAGAACTGCTAGCAGAAGAGATGATCTTGAATCTCTTGCTTATACACTTATTTTTCTCCACCGAGGGAGGTTACCATGGCAGGGCTATCAGGGTGATAACAAATCTTTCCTAGTTTGCAAAAAGAAGATGGCAACATCCCCTGAAATGCTGTGCTGCTTCTGCCCCCCACCTCTGAGACAATTTCTTGAGGTCGTGGTGAACATGAAATTTGATGAAGAGCCTAACTATTCGAAATTAATATCTTTGTTTGAGGGCTTGATTGGATCAAATCCTGCTGTAAGGCCGATTAAAACTGATGGTGCTCAAAAGATTATCAGTCAGGTTGGCCAGAAACGGGGTAGATTGAATATTGAGGAAGATGATGATGGGCAGCCAAGAAAGAAGGTTCGGCTAGGAGTACCTGCCACCCAATGGATTTCAGTTTACAATGCTCGGGTGCCGATGAAACAGAGGTATCATTACAATGTTGCTGATGCGAGGTTGGCGCAGCATGTGGAGAGAGGGATTGCAGATGGTCTGCTTATAAGTTGTGTCTCATCTTGTTCTAATCTCTGGGCACTTATTATGGACGCTGGAACCAATTTTACTAACCAAGTTTATGAGCTCTCCCCCTTCTTCTTGCACAAGGAATGGATCATGGAACAATGGGAGAAGAACTATTACATTAGTTCTATTGCTGGTGCTAATAATGGAAGTTCCCTTGTGGTGATGTCCAAAGGAACTCAGTATACGCAACAGTCTTACAAAGTAAGTGATTCTTTCCCCTTCAAGTGGATAAATAAGAAGTGGAGAGAAGGATTTCATGTGACCTCAATGGCAACTGCTGGGAGCCGGTGGGGTGTTGTTATGTCTCGCAATGCGGGCTTCAGTGATCAGGTTGTGGAACTTGATTTTCTCTATCCGAGTGAAGGCATCCACAGACGTTGGGATGGTGGTTTCCGCATAACATCAACAGCTGCAACTTGGGACCAAGCAGCTCTTATCTTGAGTGTTCCGAAGCGCAAACCTGGTGATGAAACTCAGGAGACGTTGCGTACATCTCAATTTCCAAGCACACATGTGAAGGAAAAATGGGCAAAGAATCTGTATCTCGCTTGTTTGTGTTACGGGCGCACTGTATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04392 map04011 map04392
Pfam Domains
Protein Families
Pkinase

Protein Analysis

700

Amino Acids

78.11

Weight (kDa)

9.19

Isoelectric Point (pI)

48.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 134 - 419 1.2e-16 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 135 - 405 2e-06 Protein tyrosine and serine/threonine kinase
Pkinase_fungal PF17667 249 - 343 2.1e-07 Fungal protein kinase
DUF7477 PF24289 432 - 699 1.3e-161 Domain of unknown function (DUF7477)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0011667)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13670
fragaria_vesca FvH4_3g44690
malus_domestica MD03G1011500.v1.1 MD11G1014200.v1.1
prunus_persica Prupe.6G011900_v2.0.a1 Prupe.6G011900_v2.0.a1 Prupe.6G011900_v2.0.a1
pyrus_communis pycom03g01040 pycom11g00960
rosa_chinensis RchiOBHm_Chr5g0081361
rosa_laevigata RLG00000036927
rosa_multiflora Rmu_sc0007768.1_g000010 Rmu_sc0009187.1_g000017
rosa_roxburghii Rroxscaffold_1G00002180
rosa_rugosa Rorug05G0476000
rosa_samantha Rh5AG527400 Rh5BG551600 Rh5CG574400
rosa_wichuraiana Rw5G049050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 618, 1499
Acc36I ACCTGC 1 cut(s) 1384
AccB1I GGYRCC 2 cut(s) 187, 1411
AccB7I CCANNNNNTGG 1 cut(s) 1386
AccI GTMKAC 1 cut(s) 1703
AccII CGCG 3 cut(s) 73, 79, 88
AciI CCGC 9 cut(s) 25, 68, 71, 73, 79, 111, 114, 1835, 1915
AclWI GGATC 2 cut(s) 1246, 1616
AcoI YGGCCR 2 cut(s) 451, 1300
AcsI RAATTY 3 cut(s) 515, 688, 1181
AcuI CTGAAG 2 cut(s) 678, 1825
AfaI GTAC 5 cut(s) 355, 544, 612, 1374, 2011
AfiI CCNNNNNNNGG 9 cut(s) 40, 129, 141, 373, 1055, 1309, 1386, 1746, 1883
AflIII ACRYGT 2 cut(s) 86, 2032
AgsI TTSAA 5 cut(s) 163, 380, 1004, 1321, 1744
AhlI ACTAGT 1 cut(s) 890
AjnI CCWGG 2 cut(s) 134, 1981
AleI CACNNNNGTG 1 cut(s) 2033
AluBI AGCT 8 cut(s) 10, 287, 418, 734, 833, 1579, 1931, 1952
AluI AGCT 8 cut(s) 10, 287, 418, 734, 833, 1579, 1931, 1952
Alw21I GWGCWC 3 cut(s) 525, 1279, 1581
Alw26I GTCTC 5 cut(s) 627, 1144, 1513, 1830, 1994
AlwI GGATC 2 cut(s) 1246, 1616
Ama87I CYCGRG 1 cut(s) 1407
AoxI GGCC 8 cut(s) 31, 120, 131, 310, 451, 665, 1256, 1300
ApeKI GCWGC 6 cut(s) 329, 1128, 1345, 1462, 1931, 1949
ApoI RAATTY 3 cut(s) 515, 688, 1181
AseI ATTAAT 1 cut(s) 1211
Asp700I GAANNNNTTC 2 cut(s) 375, 1773
AspLEI GCGC 4 cut(s) 361, 1462, 1977, 2088
AspS9I GGNCC 4 cut(s) 132, 551, 665, 1942
AsuHPI GGTGA 7 cut(s) 139, 238, 384, 1079, 1183, 1693, 1997
AsuII TTCGAA 1 cut(s) 1205
AsuNHI GCTAGC 1 cut(s) 983
AvaI CYCGRG 1 cut(s) 1407
AvaII GGWCC 2 cut(s) 551, 1942
BaeGI GKGCMC 1 cut(s) 1534
BaeI ACNNNNGTAYC 2 cut(s) 1412, 1445
BalI TGGCCA 1 cut(s) 1302
BanI GGYRCC 2 cut(s) 187, 1411
BanII GRGCYC 1 cut(s) 1581
BarI GAAGNNNNNNTAC 2 cut(s) 1619, 1651
BbsI GAAGAC 1 cut(s) 210
Bbv12I GWGCWC 3 cut(s) 525, 1279, 1581
BbvI GCAGC 6 cut(s) 316, 1115, 1357, 1474, 1918, 1961
BccI CCATC 7 cut(s) 286, 832, 1096, 1265, 1334, 1481, 1898
BceAI ACGGC 3 cut(s) 18, 107, 438
BcgI CGANNNNNNTGC 2 cut(s) 718, 752
BciT130I CCWGG 2 cut(s) 136, 1983
BclI TGATCA 2 cut(s) 913, 1846
BcoDI GTCTC 5 cut(s) 627, 1144, 1513, 1830, 1994
BcuI ACTAGT 1 cut(s) 890
BfaI CTAG 9 cut(s) 39, 662, 669, 755, 891, 954, 984, 1085, 1367
BfmI CTRYAG 2 cut(s) 711, 828
BfuAI ACCTGC 1 cut(s) 1384
BglI GCCNNNNNGGC 1 cut(s) 119
BisI GCNGC 9 cut(s) 71, 111, 114, 330, 1129, 1346, 1463, 1932, 1950
BlsI GCNGC 9 cut(s) 72, 112, 115, 331, 1130, 1347, 1464, 1933, 1951
BmcAI AGTACT 1 cut(s) 355
Bme1390I CCNGG 2 cut(s) 136, 1983
Bme18I GGWCC 2 cut(s) 551, 1942
BmeT110I CYCGRG 1 cut(s) 1407
BmgT120I GGNCC 4 cut(s) 132, 551, 665, 1942
BmiI GGNNCC 6 cut(s) 183, 189, 1413, 1552, 1805, 1943
BmrFI CCNGG 2 cut(s) 136, 1983
BmsI GCATC 3 cut(s) 753, 1438, 1896
BmtI GCTAGC 1 cut(s) 987
BpiI GAAGAC 1 cut(s) 210
BpmI CTGGAG 1 cut(s) 218
Bpu10I CCTNAGC 1 cut(s) 834
Bpu14I TTCGAA 1 cut(s) 1205
BpuEI CTTGAG 3 cut(s) 754, 1181, 1981
BsaBI GATNNNNATC 1 cut(s) 912
BsaJI CCNNGG 8 cut(s) 28, 71, 117, 123, 134, 862, 1036, 1049
BsaXI ACNNNNNCTCC 4 cut(s) 73, 103, 830, 860
Bsc4I CCNNNNNNNGG 9 cut(s) 40, 129, 141, 373, 1055, 1309, 1386, 1746, 1883
Bse118I RCCGGY 3 cut(s) 184, 190, 1806
Bse3DI GCAATG 1 cut(s) 1837
Bse8I GATNNNNATC 1 cut(s) 912
BseBI CCWGG 2 cut(s) 136, 1983
BseDI CCNNGG 8 cut(s) 28, 71, 117, 123, 134, 862, 1036, 1049
BseGI GGATG 4 cut(s) 685, 1109, 1887, 1909
BseJI GATNNNNATC 1 cut(s) 912
BseLI CCNNNNNNNGG 9 cut(s) 40, 129, 141, 373, 1055, 1309, 1386, 1746, 1883
BseMI GCAATG 1 cut(s) 1837
BseMII CTCAG 5 cut(s) 305, 848, 1139, 1712, 2009
BseRI GAGGAG 2 cut(s) 33, 185
BseSI GKGCMC 1 cut(s) 1534
BseXI GCAGC 6 cut(s) 316, 1115, 1357, 1474, 1918, 1961
BseYI CCCAGC 3 cut(s) 332, 418, 1799
Bsh1236I CGCG 3 cut(s) 73, 79, 88
Bsh1285I CGRYCG 1 cut(s) 77
BshFI GGCC 8 cut(s) 33, 122, 133, 312, 453, 667, 1258, 1302
BshNI GGYRCC 2 cut(s) 187, 1411
BsiEI CGRYCG 1 cut(s) 77
BsiHKAI GWGCWC 3 cut(s) 525, 1279, 1581
BsiHKCI CYCGRG 1 cut(s) 1407
BsiSI CCGG 4 cut(s) 5, 185, 191, 1807
BslFI GGGAC 1 cut(s) 1955
BslI CCNNNNNNNGG 9 cut(s) 40, 129, 141, 373, 1055, 1309, 1386, 1746, 1883
BsmAI GTCTC 5 cut(s) 627, 1144, 1513, 1830, 1994
BsmBI CGTCTC 1 cut(s) 1994
BsmFI GGGAC 1 cut(s) 1955
BsnI GGCC 8 cut(s) 33, 122, 133, 312, 453, 667, 1258, 1302
BsoBI CYCGRG 1 cut(s) 1407
Bsp119I TTCGAA 1 cut(s) 1205
Bsp1286I GDGCHC 4 cut(s) 525, 1279, 1534, 1581
Bsp143I GATC 6 cut(s) 475, 913, 997, 1238, 1608, 1846
Bsp19I CCATGG 1 cut(s) 1049
BspACI CCGC 9 cut(s) 25, 68, 71, 73, 79, 111, 114, 1835, 1915
BspANI GGCC 8 cut(s) 33, 122, 133, 312, 453, 667, 1258, 1302
BspCNI CTCAG 5 cut(s) 306, 847, 1140, 1711, 2008
BspFNI CGCG 3 cut(s) 73, 79, 88
BspLI GGNNCC 6 cut(s) 183, 189, 1413, 1552, 1805, 1943
BspMAI CTGCAG 1 cut(s) 715
BspMI ACCTGC 1 cut(s) 1384
BspOI GCTAGC 1 cut(s) 987
BspPI GGATC 2 cut(s) 1246, 1616
BspQI GCTCTTC 1 cut(s) 1185
BspT104I TTCGAA 1 cut(s) 1205
BspT107I GGYRCC 2 cut(s) 187, 1411
BsrDI GCAATG 1 cut(s) 1837
BsrFI RCCGGY 3 cut(s) 184, 190, 1806
BssAI RCCGGY 3 cut(s) 184, 190, 1806
BssECI CCNNGG 8 cut(s) 28, 71, 117, 123, 134, 862, 1036, 1049
BssMI GATC 6 cut(s) 475, 913, 997, 1238, 1608, 1846
BssNAI GTATAC 1 cut(s) 1704
BssT1I CCWWGG 2 cut(s) 862, 1049
Bst1107I GTATAC 1 cut(s) 1704
Bst2UI CCWGG 2 cut(s) 136, 1983
Bst4CI ACNGT 4 cut(s) 97, 943, 1713, 2092
Bst6I CTCTTC 4 cut(s) 162, 339, 985, 1185
BstBI TTCGAA 1 cut(s) 1205
BstC8I GCNNGC 6 cut(s) 49, 53, 90, 727, 985, 1837
BstDEI CTNAG 5 cut(s) 314, 834, 1148, 1698, 1995
BstDSI CCRYGG 3 cut(s) 71, 123, 1049
BstEII GGTNACC 2 cut(s) 390, 1044
BstF5I GGATG 4 cut(s) 685, 1109, 1887, 1909
BstFNI CGCG 3 cut(s) 73, 79, 88
BstHHI GCGC 4 cut(s) 361, 1462, 1977, 2088
BstKTI GATC 6 cut(s) 478, 916, 1000, 1241, 1611, 1849
BstMAI GTCTC 5 cut(s) 627, 1144, 1513, 1830, 1994
BstMBI GATC 6 cut(s) 475, 913, 997, 1238, 1608, 1846
BstMCI CGRYCG 1 cut(s) 77
BstNI CCWGG 2 cut(s) 136, 1983
BstNSI RCATGY 4 cut(s) 661, 905, 1469, 2036
BstPI GGTNACC 2 cut(s) 390, 1044
BstSCI CCNGG 2 cut(s) 134, 1981
BstSFI CTRYAG 2 cut(s) 711, 828
BstSLI GKGCMC 1 cut(s) 1534
BstUI CGCG 3 cut(s) 73, 79, 88
BstV1I GCAGC 6 cut(s) 316, 1115, 1357, 1474, 1918, 1961
BstV2I GAAGAC 1 cut(s) 210
BstXI CCANNNNNNTGG 1 cut(s) 564
BstZ17I GTATAC 1 cut(s) 1704
BsuRI GGCC 8 cut(s) 33, 122, 133, 312, 453, 667, 1258, 1302
BtgI CCRYGG 3 cut(s) 71, 123, 1049
BtsCI GGATG 4 cut(s) 685, 1109, 1887, 1909
BtsIMutI CAGTG 2 cut(s) 1849, 2088
BveI ACCTGC 1 cut(s) 1384
Cac8I GCNNGC 6 cut(s) 49, 53, 90, 727, 985, 1837
CfoI GCGC 4 cut(s) 361, 1462, 1977, 2088
Cfr10I RCCGGY 3 cut(s) 184, 190, 1806
Cfr13I GGNCC 4 cut(s) 132, 551, 665, 1942
Cfr42I CCGCGG 1 cut(s) 74
CseI GACGC 2 cut(s) 94, 1553
CsiI ACCWGGT 1 cut(s) 1981
Csp6I GTAC 5 cut(s) 354, 543, 611, 1373, 2010
CspCI CAANNNNNGTGG 2 cut(s) 1880, 1915
CviQI GTAC 5 cut(s) 354, 543, 611, 1373, 2010
DdeI CTNAG 5 cut(s) 314, 834, 1148, 1698, 1995
DpnI GATC 6 cut(s) 477, 915, 999, 1240, 1610, 1848
DpnII GATC 6 cut(s) 475, 913, 997, 1238, 1608, 1846
EaeI YGGCCR 2 cut(s) 451, 1300
Eam1104I CTCTTC 4 cut(s) 162, 339, 985, 1185
EarI CTCTTC 4 cut(s) 162, 339, 985, 1185
EciI GGCGGA 1 cut(s) 14
Ecl136II GAGCTC 1 cut(s) 1579
Eco130I CCWWGG 2 cut(s) 862, 1049
Eco24I GRGCYC 1 cut(s) 1581
Eco32I GATATC 1 cut(s) 222
Eco47I GGWCC 2 cut(s) 551, 1942
Eco53kI GAGCTC 1 cut(s) 1579
Eco57I CTGAAG 2 cut(s) 678, 1825
Eco88I CYCGRG 1 cut(s) 1407
Eco91I GGTNACC 2 cut(s) 390, 1044
EcoICRI GAGCTC 1 cut(s) 1579
EcoO109I RGGNCCY 1 cut(s) 665
EcoO65I GGTNACC 2 cut(s) 390, 1044
EcoRI GAATTC 1 cut(s) 688
EcoRII CCWGG 2 cut(s) 134, 1981
EcoRV GATATC 1 cut(s) 222
EcoT14I CCWWGG 2 cut(s) 862, 1049
EcoT38I GRGCYC 1 cut(s) 1581
ErhI CCWWGG 2 cut(s) 862, 1049
Esp3I CGTCTC 1 cut(s) 1994
FalI AAGNNNNNCTT 4 cut(s) 1717, 1749, 1938, 1970
FaqI GGGAC 1 cut(s) 1955
FauI CCCGC 1 cut(s) 1828
FauNDI CATATG 1 cut(s) 559
FbaI TGATCA 2 cut(s) 913, 1846
FblI GTMKAC 1 cut(s) 1703
Fnu4HI GCNGC 9 cut(s) 71, 111, 114, 330, 1129, 1346, 1463, 1932, 1950
FokI GGATG 4 cut(s) 692, 1096, 1874, 1916
FriOI GRGCYC 1 cut(s) 1581
Fsp4HI GCNGC 9 cut(s) 71, 111, 114, 330, 1129, 1346, 1463, 1932, 1950
FspBI CTAG 9 cut(s) 39, 662, 669, 755, 891, 954, 984, 1085, 1367
GlaI GCGC 4 cut(s) 360, 1461, 1976, 2087
GluI GCNGC 9 cut(s) 71, 111, 114, 330, 1129, 1346, 1463, 1932, 1950
GsaI CCCAGC 3 cut(s) 336, 422, 1803
GsuI CTGGAG 1 cut(s) 218
HaeIII GGCC 8 cut(s) 33, 122, 133, 312, 453, 667, 1258, 1302
HapII CCGG 4 cut(s) 5, 185, 191, 1807
HgaI GACGC 2 cut(s) 94, 1553
HhaI GCGC 4 cut(s) 361, 1462, 1977, 2088
Hin6I GCGC 4 cut(s) 359, 1460, 1975, 2086
HinP1I GCGC 4 cut(s) 359, 1460, 1975, 2086
HincII GTYRAC 1 cut(s) 859
HindII GTYRAC 1 cut(s) 859
HinfI GANTC 7 cut(s) 21, 233, 239, 740, 1004, 1730, 2056
HpaII CCGG 4 cut(s) 5, 185, 191, 1807
HphI GGTGA 7 cut(s) 139, 238, 384, 1079, 1183, 1693, 1997
Hpy166II GTNNAC 5 cut(s) 574, 859, 1174, 1399, 1704
Hpy188I TCNGA 6 cut(s) 61, 238, 244, 1149, 1878, 1971
Hpy188III TCNNGA 9 cut(s) 755, 771, 836, 923, 1001, 1160, 1960, 1997, 2097
Hpy8I GTNNAC 5 cut(s) 574, 859, 1174, 1399, 1704
Hpy99I CGWCG 2 cut(s) 101, 459
HpyAV CCTTC 7 cut(s) 239, 1351, 1597, 1750, 1763, 1877, 2032
HpyCH4III ACNGT 4 cut(s) 97, 943, 1713, 2092
HpyCH4IV ACGT 3 cut(s) 919, 1897, 2003
HpyCH4V TGCA 8 cut(s) 713, 729, 766, 784, 1092, 1484, 1597, 1934
HpyF3I CTNAG 5 cut(s) 314, 834, 1148, 1698, 1995
HpySE526I ACGT 3 cut(s) 919, 1897, 2003
HspAI GCGC 4 cut(s) 359, 1460, 1975, 2086
Ksp22I TGATCA 2 cut(s) 913, 1846
KspI CCGCGG 1 cut(s) 74
Kzo9I GATC 6 cut(s) 475, 913, 997, 1238, 1608, 1846
LguI GCTCTTC 1 cut(s) 1185
LmnI GCTCC 3 cut(s) 7, 181, 1803
Lsp1109I GCAGC 6 cut(s) 316, 1115, 1357, 1474, 1918, 1961
LweI GCATC 3 cut(s) 753, 1438, 1896
MabI ACCWGGT 1 cut(s) 1981
MaeI CTAG 9 cut(s) 39, 662, 669, 755, 891, 954, 984, 1085, 1367
MaeII ACGT 3 cut(s) 919, 1897, 2003
MaeIII GTNAC 5 cut(s) 390, 460, 1044, 1780, 2078
MalI GATC 6 cut(s) 477, 915, 999, 1240, 1610, 1848
MboI GATC 6 cut(s) 475, 913, 997, 1238, 1608, 1846
MhlI GDGCHC 4 cut(s) 525, 1279, 1534, 1581
MlsI TGGCCA 1 cut(s) 1302
MluCI AATT 9 cut(s) 158, 515, 688, 750, 1154, 1181, 1208, 1555, 2018
MluI ACGCGT 1 cut(s) 86
MluNI TGGCCA 1 cut(s) 1302
MlyI GAGTC 3 cut(s) 30, 227, 749
MmeI TCCRAC 1 cut(s) 84
Mox20I TGGCCA 1 cut(s) 1302
MroXI GAANNNNTTC 2 cut(s) 375, 1773
MscI TGGCCA 1 cut(s) 1302
MseI TTAA 4 cut(s) 482, 513, 1211, 1263
MslI CAYNNNNRTG 4 cut(s) 562, 789, 1437, 2033
Msp20I TGGCCA 1 cut(s) 1302
MspA1I CMGCKG 2 cut(s) 73, 1931
MspI CCGG 4 cut(s) 5, 185, 191, 1807
MspR9I CCNGG 2 cut(s) 136, 1983
MvaI CCWGG 2 cut(s) 136, 1983
MvnI CGCG 3 cut(s) 73, 79, 88
NcoI CCATGG 1 cut(s) 1049
NdeI CATATG 1 cut(s) 559
NdeII GATC 6 cut(s) 475, 913, 997, 1238, 1608, 1846
NheI GCTAGC 1 cut(s) 983
NlaIV GGNNCC 6 cut(s) 183, 189, 1413, 1552, 1805, 1943
NmeAIII GCCGAG 2 cut(s) 53, 142
NmuCI GTSAC 2 cut(s) 390, 1780
NspI RCATGY 4 cut(s) 661, 905, 1469, 2036
NspV TTCGAA 1 cut(s) 1205
OliI CACNNNNGTG 1 cut(s) 2033
PciI ACATGT 1 cut(s) 2032
PciSI GCTCTTC 1 cut(s) 1185
PdmI GAANNNNTTC 2 cut(s) 375, 1773
PfeI GAWTC 4 cut(s) 239, 1004, 1730, 2056
PflMI CCANNNNNTGG 1 cut(s) 1386
PkrI GCNGC 9 cut(s) 72, 112, 115, 331, 1130, 1347, 1464, 1933, 1951
PleI GAGTC 3 cut(s) 29, 227, 748
PpsI GAGTC 3 cut(s) 29, 227, 748
PscI ACATGT 1 cut(s) 2032
PshBI ATTAAT 1 cut(s) 1211
PsiI TTATAA 2 cut(s) 618, 1499
Psp124BI GAGCTC 1 cut(s) 1581
Psp6I CCWGG 2 cut(s) 134, 1981
PspEI GGTNACC 2 cut(s) 390, 1044
PspFI CCCAGC 3 cut(s) 332, 418, 1799
PspGI CCWGG 2 cut(s) 134, 1981
PspN4I GGNNCC 6 cut(s) 183, 189, 1413, 1552, 1805, 1943
PspPI GGNCC 4 cut(s) 132, 551, 665, 1942
PsrI GAACNNNNNNTAC 4 cut(s) 1543, 1575, 1687, 1719
PstI CTGCAG 1 cut(s) 715
PvuII CAGCTG 1 cut(s) 1931
RsaI GTAC 5 cut(s) 355, 544, 612, 1374, 2011
RsaNI GTAC 5 cut(s) 354, 543, 611, 1373, 2010
RseI CAYNNNNRTG 4 cut(s) 562, 789, 1437, 2033
SacI GAGCTC 1 cut(s) 1581
SacII CCGCGG 1 cut(s) 74
SapI GCTCTTC 1 cut(s) 1185
SaqAI TTAA 4 cut(s) 482, 513, 1211, 1263
SatI GCNGC 9 cut(s) 71, 111, 114, 330, 1129, 1346, 1463, 1932, 1950
Sau3AI GATC 6 cut(s) 475, 913, 997, 1238, 1608, 1846
Sau96I GGNCC 4 cut(s) 132, 551, 665, 1942
ScaI AGTACT 1 cut(s) 355
SchI GAGTC 3 cut(s) 30, 227, 749
ScrFI CCNGG 2 cut(s) 136, 1983
SduI GDGCHC 4 cut(s) 525, 1279, 1534, 1581
SexAI ACCWGGT 1 cut(s) 1981
SfaNI GCATC 3 cut(s) 753, 1438, 1896
SfcI CTRYAG 2 cut(s) 711, 828
Sfr303I CCGCGG 1 cut(s) 74
SfuI TTCGAA 1 cut(s) 1205
SgrBI CCGCGG 1 cut(s) 74
SinI GGWCC 2 cut(s) 551, 1942
SmiMI CAYNNNNRTG 4 cut(s) 562, 789, 1437, 2033
SmlI CTYRAG 3 cut(s) 769, 1160, 1960
SmoI CTYRAG 3 cut(s) 769, 1160, 1960
SpeI ACTAGT 1 cut(s) 890
Sse9I AATT 9 cut(s) 158, 515, 688, 750, 1154, 1181, 1208, 1555, 2018
SsiI CCGC 9 cut(s) 25, 68, 71, 73, 79, 111, 114, 1835, 1915
SspI AATATT 1 cut(s) 1324
SspMI CTAG 9 cut(s) 39, 662, 669, 755, 891, 954, 984, 1085, 1367
SstI GAGCTC 1 cut(s) 1581
StyD4I CCNGG 2 cut(s) 134, 1981
StyI CCWWGG 2 cut(s) 862, 1049
TaaI ACNGT 4 cut(s) 97, 943, 1713, 2092
TaiI ACGT 3 cut(s) 922, 1900, 2006
TaqI TCGA 4 cut(s) 270, 519, 738, 1205
TasI AATT 9 cut(s) 158, 515, 688, 750, 1154, 1181, 1208, 1555, 2018
TatI WGTACW 3 cut(s) 353, 542, 610
TauI GCSGC 3 cut(s) 73, 113, 116
TfiI GAWTC 4 cut(s) 239, 1004, 1730, 2056
Tru1I TTAA 4 cut(s) 482, 513, 1211, 1263
Tru9I TTAA 4 cut(s) 482, 513, 1211, 1263
TscAI CASTG 2 cut(s) 1849, 2095
TseFI GTSAC 2 cut(s) 390, 1780
TseI GCWGC 6 cut(s) 329, 1128, 1345, 1462, 1931, 1949
Tsp45I GTSAC 2 cut(s) 390, 1780
TspDTI ATGAA 6 cut(s) 950, 1193, 1203, 1433, 1766, 2004
TspRI CASTG 2 cut(s) 1849, 2095
Van91I CCANNNNNTGG 1 cut(s) 1386
VpaK11BI GGWCC 2 cut(s) 551, 1942
VspI ATTAAT 1 cut(s) 1211
XapI RAATTY 3 cut(s) 515, 688, 1181
XbaI TCTAGA 1 cut(s) 754
XceI RCATGY 4 cut(s) 661, 905, 1469, 2036
XcmI CCANNNNNNNNNTGG 1 cut(s) 142
XmiI GTMKAC 1 cut(s) 1703
XmnI GAANNNNTTC 2 cut(s) 375, 1773
XspI CTAG 9 cut(s) 39, 662, 669, 755, 891, 954, 984, 1085, 1367
ZrmI AGTACT 1 cut(s) 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.