Rorug05G0476000

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0006538
Physical Location & Seq
Forward (+)
65454325 .. 65515867
61543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0476000.1

Sequence Viewer

Length: 3096 bp
ATGGATTCCCACTTCCATACTCTACTGCCTCCTCAGTTTTCCATGTTAGCTCTTCTTCTATGCATATCATTTCTGAGCGCAGAGTCTGCTATAATGCCAAGCATTACCACCGACAAGGAGGCCTTGATCTCATTCAAGGAGGCCTTGCTCGATGTCCCTCCATCTTTAAGCTGGGACCAGAATTCATCCCCTTGCAGCAATTGGACCGGAGTTGTTTGCAGAAAACGTGGTCAGAGTCAGAGAGTTGTTGTTCTTGATCTTTCAGGATTGGGACTTACAGGGTCCATAAGCCCTCACATTGGTAACTTATCCTTCCTCCAATCCCTTCAACTTCAAAACAACAAGTTAACAGGAGCAATTCCTACTCAAGTTGTTAACCTTTTCCGCTTGAGATCTCTGAACCTGAGCTCCAACACCATACAAGGTCCACTACCTTCAAACATGAGCCACTTGACTGCACTAGAAATGCTCGACTTGGCGGGGAATAATATAACAGGGAAGCTCCCTGAGGAGATATTCAGCCAAAAAGAACTTCAAGTCCTGAATCTGGGACGAAACAATTTCTTTGGTTCAATTCCTTCATCCATAAGCAACCTCTCATCCACGCTCACATATTTGAATTTGGGTACAAATAGCCTCAGTGGGATCATACCTAGTGAGTTGGGTCTACTTATCAATCTCAAGGAACTTGATCTTTCTGGTAACAAGTTCACTGGGACTGTTGCTCCCTCCATATACAACATTACTTCATTGGTTCTGTTTACTGTTGCTTCAAATCAACTTTGGGGTGAGATTCCCAAAAATATTGATGAAACACTTCCAAATCTTTTATACTACCGGAACTGCTTTAATCTTATGACAGGGAAAATTCCAGTGTCTTTGCACAATATCACCAAGATTCGATCAATTCGAATGTCAAACAATTTTCTGGAAGGAACAGTTCCACCAGGGCTGGGAAACATGCCATTCCTCGAAATGTACAACATTGGCTTCAATCGGATCGTCAGTAAAGGCAGTGATGGTCTCAGTTTTATCACTTCCTTGACGAACAGCACCAACCTGCAATTTCTTGCTATCGACGACAATCAGTTAGAGGGAGTCATTCCAGAATCACTTGGCAATCTCTCAAAGGTTCTCTCAAAATTGTACATGGGAGGGAATCGGATTTATGGTAACATTCCTGCCTCAGTTGGTCATCTTAGTGGTTTAACTTTGTTAAATGTGAGCTACAATTCAATATCTGGTGAAATTCCCACTGAAATAGGCCAATTGGAAAACCTACAAATGCTTGGTTTAGCAGGAAATGAAATGTCTGGTCATATTCCAAACTCTTTAGGAAATCTCAAAAAGTTGAACGGTATTGATTTATCTGGGAATCACTTTGTCGGCCAAATACCCTCTTCTTTTTCAAACTTCCAGAGTTTGCTTTCCATGGACTTGTCAAACAATCAGCTCAACGGAACCATTTCCGGTGAAACTCTCAACCTTCCTAGTTTGAGCACCATTCTCAATCTGTCCAAAAACTTTCTAAGTGGACCTTTGCCTTCAGAAATTGGGTCCCTTGAAAAAGTTGTCACTATAGACCTCTCTGACAATGCATTATCTGGAGATATCCCTGGATCAATAGGAAAGTGTAAAAGCTTGGGGAGATTGTTGATGTCAAGAAATAGAATTTCAGGTCCTATTTCCACTGGTCTAGGAGAATTGAGAGGCCTTGAATTTCTAGACCTCTCCTCAAACCAGCTGTCCAGCTCCATTCCAGAGAATTTTCGAGACCTACGTGCCCTTCGATACTTGAACCTATCATTTAATAACTTAGAAGGAGTGATTCCTAATGGTGGAGTATTTGTTACAGATTTCACTAATGTTCATCTTGAAGGCAACCCAAAAATTTGCCTCAAATTTCCATGTGAGAAAAATACTGATGGTCATCGAAGAAGAAAAATACTAGTCCTAGTTATGATCATTACAACAGTCTTAGCAACCATATGTGTTATAGTGGGCTCTTTGGTCTATGCGAAGACAAGAAAAGGCTGCAAAGCAAAAATTACAGGAACTTGTGATAACGTAGTAGTAAAAGGGCAGCATCAGATGGTATCGTATGAAGAACTCCGTCGCTCAACAGGGAATTTCAACCCGGATAATTTGGTTGGAAGGGGGAGCTTTGGTTCTGTTTATAAAGGGTTTCTTAGAGACCAAGGAATTGAAGTTGCAGTCAAGGTGCTCGATATAAGAACAACAGGGTCTTGGAAGAGTTTCTTGGCAGAGTGTAATGCATTAAGGAGTGTGAGGCACCGAAATCTTGTCAAACTGATCACCTCCTGCTCAAGCTTAGACTGTAAGAACATGGAGTTTCTGGCTTTGGTTTATGAGTATCTGAGTAATGGGAGCTTGGAAGATTGGATTAAGCGCAAGAGAACAAATTCAGATGGAAACGGTTTGAACATTGTGGAGAGATTGAATGTGGCAATTGATGTTGCTTGTGGATTGGATTACCTCCATAATGATTGTGAAGTTGCAGTGGCTCACTGTGATTTGAAGCCAAGCAACATTCTTTTGGACAAAGACATGACTGCAAAGGTTGGAGATTTTGGGCTGGCAAAGTTACTGATACAGGAAACATCCACTCAACATTCTATTGGCTCCACCAATGTCCTCAAGGGTTCCATAGGATATATACCGCCAGAATATGGTTTTGGACAAAAACCCTCCACAGCTGGAGATGCATACAGTTTTGGAGTAATGTTGTTGGAGCTGTTTACTGGAAAAAGTCCAACCGATGAAAGCTTCACAGGAGAAGCTAACCTCGTTCAATGGGTGCTGTCATCTTTTCCCCACAACATAGCACAAGTACTAGACTCTCAGTTGTCACATTATGCTGAGCACGACGAAGACTCAAATCCCATCTCATCAGAAGAACTGAATTGTCTGATCTCAGTTATTGAGGTTGGGCTCTCTTGCACCTTCGCCTCTCCTGATGGACGGATTAGCTTGAGGGATGCCCTTCACAAACTTCAAACTGCTAGAGAGACGCTTTTGAAGCTTGCTCACTTTGACAGTGCAGCGTGTGAATTTCAACAACAATTGGAGTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko05206 map00270 map01100 map05206
Pfam Domains
Protein Families
BAH Chromo DNA_methylase

Protein Analysis

1031

Amino Acids

112.13

Weight (kDa)

6.03

Isoelectric Point (pI)

36.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 37 - 73 1.2e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 95 - 159 8.2e-08 Leucine-rich repeat region
LRR_8 PF13855 106 - 164 5.5e-09 Leucine rich repeat
LRR_8 PF13855 177 - 237 3.2e-06 Leucine rich repeat
LRR_14 PF23598 345 - 456 1.1e-08 Leucine-rich repeat region
LRR_8 PF13855 379 - 436 3.9e-06 Leucine rich repeat
LRR_14 PF23598 467 - 605 7.8e-10 Leucine-rich repeat region
LRR_8 PF13855 569 - 606 5.1e-07 Leucine rich repeat
Pkinase PF00069 714 - 938 2.6e-41 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 715 - 928 2.7e-39 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2178
Acc36I ACCTGC 1 cut(s) 1068
AccB1I GGYRCC 1 cut(s) 2292
AccB7I CCANNNNNTGG 1 cut(s) 2690
AccI GTMKAC 1 cut(s) 667
AciI CCGC 3 cut(s) 385, 479, 2681
AclWI GGATC 3 cut(s) 653, 1007, 1627
AcoI YGGCCR 1 cut(s) 1387
AcuI CTGAAG 1 cut(s) 1530
AfaI GTAC 4 cut(s) 628, 980, 1148, 2853
AfiI CCNNNNNNNGG 5 cut(s) 299, 547, 953, 1838, 2690
AhlI ACTAGT 1 cut(s) 1948
AjnI CCWGG 2 cut(s) 946, 1615
AjuI GAANNNNNNNTTGG 6 cut(s) 516, 548, 2243, 2275, 2679, 2711
AloI GAACNNNNNNTCC 6 cut(s) 392, 424, 522, 554, 2152, 2184
Alw21I GWGCWC 4 cut(s) 410, 1502, 2226, 2886
Alw26I GTCTC 4 cut(s) 1028, 1767, 2187, 3023
AlwI GGATC 3 cut(s) 653, 1007, 1627
AlwNI CAGNNNCTG 1 cut(s) 86
AoxI GGCC 5 cut(s) 120, 141, 1264, 1387, 1711
ApeKI GCWGC 4 cut(s) 195, 2034, 2083, 3062
ArsI GACNNNNNNTTYG 2 cut(s) 1730, 1762
Asp700I GAANNNNTTC 4 cut(s) 816, 1465, 2255, 2422
AspLEI GCGC 2 cut(s) 80, 2412
AspS9I GGNCC 7 cut(s) 175, 204, 282, 425, 1535, 1557, 1679
AsuC2I CCSGG 1 cut(s) 2138
AsuHPI GGTGA 5 cut(s) 800, 883, 1256, 1484, 2308
AsuII TTCGAA 1 cut(s) 910
AvaII GGWCC 7 cut(s) 175, 204, 282, 425, 1535, 1557, 1679
AxyI CCTNAGG 1 cut(s) 507
BaeGI GKGCMC 1 cut(s) 1786
BanI GGYRCC 1 cut(s) 2292
BanII GRGCYC 3 cut(s) 410, 2006, 2955
BbsI GAAGAC 2 cut(s) 2027, 2898
Bbv12I GWGCWC 4 cut(s) 410, 1502, 2226, 2886
BbvI GCAGC 4 cut(s) 207, 2021, 2095, 3074
BccI CCATC 7 cut(s) 169, 1013, 1919, 2086, 2423, 2912, 2972
BciT130I CCWGG 2 cut(s) 948, 1617
BclI TGATCA 2 cut(s) 1962, 2313
BcnI CCSGG 1 cut(s) 2138
BcoDI GTCTC 4 cut(s) 1028, 1767, 2187, 3023
BcuI ACTAGT 1 cut(s) 1948
BfaI CTAG 9 cut(s) 461, 654, 1491, 1697, 1724, 1949, 1955, 2855, 3024
BfmI CTRYAG 1 cut(s) 1578
BfuAI ACCTGC 1 cut(s) 1068
BglII AGATCT 1 cut(s) 392
BisI GCNGC 4 cut(s) 196, 2035, 2084, 3063
BlpI GCTNAGC 1 cut(s) 2880
BlsI GCNGC 4 cut(s) 197, 2036, 2085, 3064
BmcAI AGTACT 1 cut(s) 2853
Bme1390I CCNGG 3 cut(s) 948, 1617, 2138
Bme18I GGWCC 7 cut(s) 175, 204, 282, 425, 1535, 1557, 1679
BmgT120I GGNCC 7 cut(s) 175, 204, 282, 425, 1535, 1557, 1679
BmiI GGNNCC 8 cut(s) 176, 283, 1462, 1558, 1559, 2294, 2644, 2665
BmrFI CCNGG 3 cut(s) 948, 1617, 2138
BmrI ACTGGG 1 cut(s) 723
BmsI GCATC 3 cut(s) 2095, 2713, 2989
BmuI ACTGGG 1 cut(s) 723
BpiI GAAGAC 2 cut(s) 2027, 2898
BpmI CTGGAG 2 cut(s) 1626, 2739
Bpu10I CCTNAGC 1 cut(s) 404
Bpu1102I GCTNAGC 1 cut(s) 2880
Bpu14I TTCGAA 1 cut(s) 910
BpuEI CTTGAG 6 cut(s) 351, 409, 665, 2311, 2642, 3013
BpuMI CCSGG 1 cut(s) 2138
BsaAI YACGTR 1 cut(s) 1781
BsaBI GATNNNNATC 1 cut(s) 1929
BsaI GGTCTC 3 cut(s) 1028, 1767, 2187
BsaJI CCNNGG 4 cut(s) 947, 1431, 1615, 2197
BsaWI WCCGGW 3 cut(s) 206, 837, 1469
BsaXI ACNNNNNCTCC 8 cut(s) 392, 422, 709, 739, 1833, 1863, 2152, 2182
Bsc4I CCNNNNNNNGG 5 cut(s) 299, 547, 953, 1838, 2690
Bse1I ACTGG 4 cut(s) 718, 872, 1696, 2767
Bse21I CCTNAGG 1 cut(s) 507
Bse8I GATNNNNATC 1 cut(s) 1929
BseBI CCWGG 2 cut(s) 948, 1617
BseDI CCNNGG 4 cut(s) 947, 1431, 1615, 2197
BseGI GGATG 5 cut(s) 185, 581, 599, 2621, 3004
BseJI GATNNNNATC 1 cut(s) 1929
BseLI CCNNNNNNNGG 5 cut(s) 299, 547, 953, 1838, 2690
BseNI ACTGG 4 cut(s) 718, 872, 1696, 2767
BseRI GAGGAG 3 cut(s) 21, 524, 1723
BseSI GKGCMC 1 cut(s) 1786
BseXI GCAGC 4 cut(s) 207, 2021, 2095, 3074
BseYI CCCAGC 2 cut(s) 171, 952
BsgI GTGCAG 2 cut(s) 441, 3081
BshFI GGCC 5 cut(s) 122, 143, 1266, 1389, 1713
BshNI GGYRCC 1 cut(s) 2292
BsiHKAI GWGCWC 4 cut(s) 410, 1502, 2226, 2886
BsiSI CCGG 4 cut(s) 207, 838, 1470, 2138
BslFI GGGAC 6 cut(s) 140, 188, 285, 564, 730, 1543
BslI CCNNNNNNNGG 5 cut(s) 299, 547, 953, 1838, 2690
BsmAI GTCTC 4 cut(s) 1028, 1767, 2187, 3023
BsmBI CGTCTC 1 cut(s) 3023
BsmFI GGGAC 6 cut(s) 140, 188, 285, 564, 730, 1543
BsnI GGCC 5 cut(s) 122, 143, 1266, 1389, 1713
Bso31I GGTCTC 3 cut(s) 1028, 1767, 2187
Bsp119I TTCGAA 1 cut(s) 910
Bsp1286I GDGCHC 7 cut(s) 410, 1502, 1786, 2006, 2226, 2886, 2955
Bsp1407I TGTACA 2 cut(s) 978, 1146
Bsp1720I GCTNAGC 1 cut(s) 2880
Bsp19I CCATGG 1 cut(s) 1431
BspACI CCGC 3 cut(s) 385, 479, 2681
BspANI GGCC 5 cut(s) 122, 143, 1266, 1389, 1713
BspLI GGNNCC 8 cut(s) 176, 283, 1462, 1558, 1559, 2294, 2644, 2665
BspMI ACCTGC 1 cut(s) 1068
BspPI GGATC 3 cut(s) 653, 1007, 1627
BspQI GCTCTTC 1 cut(s) 57
BspT104I TTCGAA 1 cut(s) 910
BspT107I GGYRCC 1 cut(s) 2292
BspTNI GGTCTC 3 cut(s) 1028, 1767, 2187
BsrGI TGTACA 2 cut(s) 978, 1146
BsrI ACTGG 4 cut(s) 718, 872, 1696, 2767
BssECI CCNNGG 4 cut(s) 947, 1431, 1615, 2197
BssT1I CCWWGG 2 cut(s) 1431, 2197
Bst2UI CCWGG 2 cut(s) 948, 1617
Bst6I CTCTTC 3 cut(s) 57, 1405, 2246
BstAPI GCANNNNNTGC 1 cut(s) 86
BstAUI TGTACA 2 cut(s) 978, 1146
BstBAI YACGTR 1 cut(s) 1781
BstBI TTCGAA 1 cut(s) 910
BstC8I GCNNGC 2 cut(s) 2598, 3045
BstDSI CCRYGG 1 cut(s) 1431
BstF5I GGATG 5 cut(s) 185, 581, 599, 2621, 3004
BstHHI GCGC 2 cut(s) 80, 2412
BstMAI GTCTC 4 cut(s) 1028, 1767, 2187, 3023
BstMWI GCNNNNNNNGC 3 cut(s) 86, 2723, 3040
BstNI CCWGG 2 cut(s) 948, 1617
BstNSI RCATGY 1 cut(s) 964
BstSCI CCNGG 3 cut(s) 946, 1615, 2136
BstSFI CTRYAG 1 cut(s) 1578
BstSLI GKGCMC 1 cut(s) 1786
BstV1I GCAGC 4 cut(s) 207, 2021, 2095, 3074
BstV2I GAAGAC 2 cut(s) 2027, 2898
BstX2I RGATCY 1 cut(s) 392
BstYI RGATCY 1 cut(s) 392
Bsu36I CCTNAGG 1 cut(s) 507
BsuRI GGCC 5 cut(s) 122, 143, 1266, 1389, 1713
BtgI CCRYGG 1 cut(s) 1431
BtsCI GGATG 5 cut(s) 185, 581, 599, 2621, 3004
BtsI GCAGTG 2 cut(s) 1021, 2526
BtsIMutI CAGTG 9 cut(s) 646, 711, 879, 1021, 1254, 1689, 2526, 2527, 3064
BveI ACCTGC 1 cut(s) 1068
Cac8I GCNNGC 2 cut(s) 2598, 3045
CaiI CAGNNNCTG 1 cut(s) 86
CfoI GCGC 2 cut(s) 80, 2412
Cfr13I GGNCC 7 cut(s) 175, 204, 282, 425, 1535, 1557, 1679
CseI GACGC 1 cut(s) 3040
Csp6I GTAC 4 cut(s) 627, 979, 1147, 2852
CviAII CATG 8 cut(s) 43, 442, 961, 1150, 1432, 1908, 2347, 2569
CviQI GTAC 4 cut(s) 627, 979, 1147, 2852
EaeI YGGCCR 1 cut(s) 1387
Eam1104I CTCTTC 3 cut(s) 57, 1405, 2246
EarI CTCTTC 3 cut(s) 57, 1405, 2246
Ecl136II GAGCTC 1 cut(s) 408
Eco130I CCWWGG 2 cut(s) 1431, 2197
Eco147I AGGCCT 3 cut(s) 122, 143, 1713
Eco24I GRGCYC 3 cut(s) 410, 2006, 2955
Eco31I GGTCTC 3 cut(s) 1028, 1767, 2187
Eco32I GATATC 1 cut(s) 1612
Eco47I GGWCC 7 cut(s) 175, 204, 282, 425, 1535, 1557, 1679
Eco53kI GAGCTC 1 cut(s) 408
Eco57I CTGAAG 1 cut(s) 1530
Eco81I CCTNAGG 1 cut(s) 507
EcoICRI GAGCTC 1 cut(s) 408
EcoO109I RGGNCCY 2 cut(s) 1557, 1679
EcoRI GAATTC 1 cut(s) 181
EcoRII CCWGG 2 cut(s) 946, 1615
EcoRV GATATC 1 cut(s) 1612
EcoT14I CCWWGG 2 cut(s) 1431, 2197
EcoT22I ATGCAT 4 cut(s) 65, 1600, 2278, 2728
EcoT38I GRGCYC 3 cut(s) 410, 2006, 2955
ErhI CCWWGG 2 cut(s) 1431, 2197
Esp3I CGTCTC 1 cut(s) 3023
FaeI CATG 8 cut(s) 46, 445, 964, 1153, 1435, 1911, 2350, 2572
FaqI GGGAC 6 cut(s) 140, 188, 285, 564, 730, 1543
FatI CATG 8 cut(s) 42, 441, 960, 1149, 1431, 1907, 2346, 2568
FauI CCCGC 1 cut(s) 472
FauNDI CATATG 1 cut(s) 1988
FbaI TGATCA 2 cut(s) 1962, 2313
FblI GTMKAC 1 cut(s) 667
Fnu4HI GCNGC 4 cut(s) 196, 2035, 2084, 3063
FokI GGATG 5 cut(s) 172, 568, 586, 2608, 3011
FriOI GRGCYC 3 cut(s) 410, 2006, 2955
Fsp4HI GCNGC 4 cut(s) 196, 2035, 2084, 3063
FspBI CTAG 9 cut(s) 461, 654, 1491, 1697, 1724, 1949, 1955, 2855, 3024
GlaI GCGC 2 cut(s) 79, 2411
GluI GCNGC 4 cut(s) 196, 2035, 2084, 3063
GsaI CCCAGC 2 cut(s) 175, 956
GsuI CTGGAG 2 cut(s) 1626, 2739
HaeIII GGCC 5 cut(s) 122, 143, 1266, 1389, 1713
HapII CCGG 4 cut(s) 207, 838, 1470, 2138
HgaI GACGC 1 cut(s) 3040
HhaI GCGC 2 cut(s) 80, 2412
Hin1II CATG 8 cut(s) 46, 445, 964, 1153, 1435, 1911, 2350, 2572
Hin6I GCGC 2 cut(s) 78, 2410
HinP1I GCGC 2 cut(s) 78, 2410
HincII GTYRAC 2 cut(s) 348, 376
HindII GTYRAC 2 cut(s) 348, 376
HindIII AAGCTT 4 cut(s) 1639, 2329, 2785, 3041
HpaI GTTAAC 2 cut(s) 348, 376
HpaII CCGG 4 cut(s) 207, 838, 1470, 2138
HphI GGTGA 5 cut(s) 800, 883, 1256, 1484, 2308
Hpy166II GTNNAC 8 cut(s) 348, 376, 428, 668, 711, 762, 1535, 2760
Hpy8I GTNNAC 8 cut(s) 348, 376, 428, 668, 711, 762, 1535, 2760
Hpy99I CGWCG 3 cut(s) 1082, 2118, 2891
HpyCH4IV ACGT 3 cut(s) 226, 1780, 2067
HpyF10VI GCNNNNNNNGC 3 cut(s) 86, 2723, 3040
HpySE526I ACGT 3 cut(s) 226, 1780, 2067
Hsp92II CATG 8 cut(s) 46, 445, 964, 1153, 1435, 1911, 2350, 2572
HspAI GCGC 2 cut(s) 78, 2410
KflI GGGWCCC 1 cut(s) 1557
Ksp22I TGATCA 2 cut(s) 1962, 2313
KspAI GTTAAC 2 cut(s) 348, 376
LguI GCTCTTC 1 cut(s) 57
LmnI GCTCC 9 cut(s) 353, 413, 507, 730, 1757, 2160, 2388, 2648, 2752
Lsp1109I GCAGC 4 cut(s) 207, 2021, 2095, 3074
LweI GCATC 3 cut(s) 2095, 2713, 2989
MaeI CTAG 9 cut(s) 461, 654, 1491, 1697, 1724, 1949, 1955, 2855, 3024
MaeII ACGT 3 cut(s) 226, 1780, 2067
MaeIII GTNAC 7 cut(s) 302, 701, 1172, 1573, 1849, 2604, 2868
MfeI CAATTG 4 cut(s) 199, 1268, 2469, 3083
MflI RGATCY 1 cut(s) 392
MhlI GDGCHC 7 cut(s) 410, 1502, 1786, 2006, 2226, 2886, 2955
MlyI GAGTC 5 cut(s) 92, 244, 1107, 2852, 2888
MmeI TCCRAC 5 cut(s) 435, 2131, 2563, 2730, 2798
Mph1103I ATGCAT 4 cut(s) 65, 1600, 2278, 2728
MroXI GAANNNNTTC 4 cut(s) 816, 1465, 2255, 2422
MseI TTAA 9 cut(s) 167, 347, 375, 849, 1208, 1217, 1809, 2279, 2406
MslI CAYNNNNRTG 1 cut(s) 1200
MspA1I CMGCKG 2 cut(s) 1744, 2717
MspI CCGG 4 cut(s) 207, 838, 1470, 2138
MspR9I CCNGG 3 cut(s) 948, 1617, 2138
MunI CAATTG 4 cut(s) 199, 1268, 2469, 3083
MvaI CCWGG 2 cut(s) 948, 1617
MwoI GCNNNNNNNGC 3 cut(s) 86, 2723, 3040
NciI CCSGG 1 cut(s) 2138
NcoI CCATGG 1 cut(s) 1431
NdeI CATATG 1 cut(s) 1988
NlaIII CATG 8 cut(s) 46, 445, 964, 1153, 1435, 1911, 2350, 2572
NlaIV GGNNCC 8 cut(s) 176, 283, 1462, 1558, 1559, 2294, 2644, 2665
NmuCI GTSAC 2 cut(s) 1573, 2868
NsiI ATGCAT 4 cut(s) 65, 1600, 2278, 2728
NspI RCATGY 1 cut(s) 964
NspV TTCGAA 1 cut(s) 910
PceI AGGCCT 3 cut(s) 122, 143, 1713
PciSI GCTCTTC 1 cut(s) 57
PcsI WCGNNNNNNNCGW 3 cut(s) 907, 1777, 1786
PdmI GAANNNNTTC 4 cut(s) 816, 1465, 2255, 2422
PfeI GAWTC 8 cut(s) 5, 544, 793, 898, 1109, 1159, 1375, 1828
PflMI CCANNNNNTGG 1 cut(s) 2690
PkrI GCNGC 4 cut(s) 197, 2036, 2085, 3064
PleI GAGTC 5 cut(s) 91, 243, 1106, 2852, 2888
PpsI GAGTC 5 cut(s) 91, 243, 1106, 2852, 2888
Ppu21I YACGTR 1 cut(s) 1781
PpuMI RGGWCCY 2 cut(s) 1557, 1679
PsiI TTATAA 1 cut(s) 2178
Psp124BI GAGCTC 1 cut(s) 410
Psp5II RGGWCCY 2 cut(s) 1557, 1679
Psp6I CCWGG 2 cut(s) 946, 1615
PspFI CCCAGC 2 cut(s) 171, 952
PspGI CCWGG 2 cut(s) 946, 1615
PspN4I GGNNCC 8 cut(s) 176, 283, 1462, 1558, 1559, 2294, 2644, 2665
PspPI GGNCC 7 cut(s) 175, 204, 282, 425, 1535, 1557, 1679
PspPPI RGGWCCY 2 cut(s) 1557, 1679
PstNI CAGNNNCTG 1 cut(s) 86
PsuI RGATCY 1 cut(s) 392
PvuII CAGCTG 2 cut(s) 1744, 2717
RsaI GTAC 4 cut(s) 628, 980, 1148, 2853
RsaNI GTAC 4 cut(s) 627, 979, 1147, 2852
RseI CAYNNNNRTG 1 cut(s) 1200
SacI GAGCTC 1 cut(s) 410
SapI GCTCTTC 1 cut(s) 57
SaqAI TTAA 9 cut(s) 167, 347, 375, 849, 1208, 1217, 1809, 2279, 2406
SatI GCNGC 4 cut(s) 196, 2035, 2084, 3063
Sau96I GGNCC 7 cut(s) 175, 204, 282, 425, 1535, 1557, 1679
ScaI AGTACT 1 cut(s) 2853
SchI GAGTC 5 cut(s) 92, 244, 1107, 2852, 2888
ScrFI CCNGG 3 cut(s) 948, 1617, 2138
SduI GDGCHC 7 cut(s) 410, 1502, 1786, 2006, 2226, 2886, 2955
SfaNI GCATC 3 cut(s) 2095, 2713, 2989
SfcI CTRYAG 1 cut(s) 1578
SfuI TTCGAA 1 cut(s) 910
SinI GGWCC 7 cut(s) 175, 204, 282, 425, 1535, 1557, 1679
SmiMI CAYNNNNRTG 1 cut(s) 1200
SmlI CTYRAG 6 cut(s) 366, 388, 680, 2326, 2657, 2992
SmoI CTYRAG 6 cut(s) 366, 388, 680, 2326, 2657, 2992
SpeI ACTAGT 1 cut(s) 1948
SseBI AGGCCT 3 cut(s) 122, 143, 1713
SsiI CCGC 3 cut(s) 385, 479, 2681
SspI AATATT 1 cut(s) 805
SspMI CTAG 9 cut(s) 461, 654, 1491, 1697, 1724, 1949, 1955, 2855, 3024
SstI GAGCTC 1 cut(s) 410
StuI AGGCCT 3 cut(s) 122, 143, 1713
StyD4I CCNGG 3 cut(s) 946, 1615, 2136
StyI CCWWGG 2 cut(s) 1431, 2197
TaiI ACGT 3 cut(s) 229, 1783, 2070
TatI WGTACW 3 cut(s) 978, 1146, 2851
TfiI GAWTC 8 cut(s) 5, 544, 793, 898, 1109, 1159, 1375, 1828
Tru1I TTAA 9 cut(s) 167, 347, 375, 849, 1208, 1217, 1809, 2279, 2406
Tru9I TTAA 9 cut(s) 167, 347, 375, 849, 1208, 1217, 1809, 2279, 2406
TscAI CASTG 9 cut(s) 646, 718, 879, 1021, 1261, 1696, 2526, 2534, 3064
TseFI GTSAC 2 cut(s) 1573, 2868
TseI GCWGC 4 cut(s) 195, 2034, 2083, 3062
Tsp45I GTSAC 2 cut(s) 1573, 2868
TspDTI ATGAA 8 cut(s) 174, 570, 738, 825, 1320, 1859, 2118, 2796
TspGWI ACGGA 3 cut(s) 1473, 2102, 2998
TspRI CASTG 9 cut(s) 646, 718, 879, 1021, 1261, 1696, 2526, 2534, 3064
Van91I CCANNNNNTGG 1 cut(s) 2690
VpaK11BI GGWCC 7 cut(s) 175, 204, 282, 425, 1535, 1557, 1679
XbaI TCTAGA 1 cut(s) 1723
XceI RCATGY 1 cut(s) 964
XcmI CCANNNNNNNNNTGG 1 cut(s) 168
XmiI GTMKAC 1 cut(s) 667
XmnI GAANNNNTTC 4 cut(s) 816, 1465, 2255, 2422
XspI CTAG 9 cut(s) 461, 654, 1491, 1697, 1724, 1949, 1955, 2855, 3024
ZrmI AGTACT 1 cut(s) 2853
Zsp2I ATGCAT 4 cut(s) 65, 1600, 2278, 2728
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.