MD03G1010900.v1.1

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0006538
Physical Location & Seq
Reverse (-)
821688 .. 822170
483 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1010900.v1.1.491

Sequence Viewer

Length: 483 bp
ATGGAAAATGGGACAAAAGTTACACAAGTGCGCGTCTCAATTATTTTTCTTGCTGTGCAGTGCACTGTACACACCTCGTTCCACCAGAGTAACTACATAAAGGCTTTCACTCTCTGCACGCTTCACTATTTGCTCATGGCTTCAGCTTCCAGATCAAGCACTTGGCTTTTCAGTCTGTCGGAGAATTGGCTCCCAATGGCTACTATAATATTCGCTTGCGGGTTTGTCGGTTACGCTGTTTACGACGCAGTGATGGCCACCGTGTCTGAGCTGCTGCAACGTTTGTTGGTAATTTCTCCTTTACTTATAGTCATTGTAGTTCATTGGCTCTCCACAGGCAGCCAAGTTAACATTAGCATTCCGGGGTCGGAGCCCGGAGCCATCCATAGAGCCGGGGGATCGCCTTGGGGTGTGGCCTTTGTACTATTTCTGCTCTTCTTTCTCATATCTTACCAGCCTTCCTTGCATGGCCTTCTTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko05206 map00270 map01100 map05206
Pfam Domains
Protein Families
BAH Chromo DNA_methylase

Protein Analysis

161

Amino Acids

17.61

Weight (kDa)

7.81

Isoelectric Point (pI)

43.47

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 33
AciI CCGC 1 cut(s) 219
AclI AACGTT 1 cut(s) 280
AclWI GGATC 1 cut(s) 406
AcoI YGGCCR 1 cut(s) 255
AcuI CTGAAG 1 cut(s) 126
AfaI GTAC 2 cut(s) 69, 423
AluBI AGCT 2 cut(s) 146, 271
AluI AGCT 2 cut(s) 146, 271
Alw21I GWGCWC 1 cut(s) 65
Alw26I GTCTC 1 cut(s) 40
Alw44I GTGCAC 1 cut(s) 61
AlwI GGATC 1 cut(s) 406
AoxI GGCC 3 cut(s) 255, 414, 469
ApaLI GTGCAC 1 cut(s) 61
ApeKI GCWGC 3 cut(s) 271, 274, 339
AspLEI GCGC 1 cut(s) 33
AsuC2I CCSGG 3 cut(s) 363, 375, 394
BaeGI GKGCMC 1 cut(s) 65
BalI TGGCCA 1 cut(s) 257
BanII GRGCYC 1 cut(s) 375
Bbv12I GWGCWC 1 cut(s) 65
BbvI GCAGC 3 cut(s) 258, 261, 351
BccI CCATC 2 cut(s) 247, 389
BcnI CCSGG 3 cut(s) 363, 375, 394
BcoDI GTCTC 1 cut(s) 40
BfaI CTAG 1 cut(s) 481
BisI GCNGC 3 cut(s) 272, 275, 340
BlsI GCNGC 3 cut(s) 273, 276, 341
Bme1390I CCNGG 3 cut(s) 363, 375, 394
BmiI GGNNCC 3 cut(s) 191, 372, 379
BmrFI CCNGG 3 cut(s) 363, 375, 394
BpuMI CCSGG 3 cut(s) 363, 375, 394
BsaJI CCNNGG 3 cut(s) 362, 393, 404
BseDI CCNNGG 3 cut(s) 362, 393, 404
BseGI GGATG 1 cut(s) 381
BseMII CTCAG 1 cut(s) 258
BseSI GKGCMC 1 cut(s) 65
BseXI GCAGC 3 cut(s) 258, 261, 351
BsgI GTGCAG 2 cut(s) 77, 100
Bsh1236I CGCG 1 cut(s) 33
BshFI GGCC 3 cut(s) 257, 416, 471
BsiHKAI GWGCWC 1 cut(s) 65
BsiSI CCGG 3 cut(s) 362, 375, 393
BslFI GGGAC 1 cut(s) 25
BsmAI GTCTC 1 cut(s) 40
BsmBI CGTCTC 1 cut(s) 40
BsmFI GGGAC 1 cut(s) 25
BsmI GAATGC 1 cut(s) 357
BsnI GGCC 3 cut(s) 257, 416, 471
Bsp1286I GDGCHC 2 cut(s) 65, 375
Bsp1407I TGTACA 1 cut(s) 67
Bsp143I GATC 2 cut(s) 152, 398
BspACI CCGC 1 cut(s) 219
BspANI GGCC 3 cut(s) 257, 416, 471
BspCNI CTCAG 1 cut(s) 259
BspFNI CGCG 1 cut(s) 33
BspLI GGNNCC 3 cut(s) 191, 372, 379
BspPI GGATC 1 cut(s) 406
BspQI GCTCTTC 1 cut(s) 440
BsrGI TGTACA 1 cut(s) 67
BssECI CCNNGG 3 cut(s) 362, 393, 404
BssMI GATC 2 cut(s) 152, 398
BssT1I CCWWGG 1 cut(s) 404
Bst4CI ACNGT 2 cut(s) 67, 262
Bst6I CTCTTC 1 cut(s) 440
BstAUI TGTACA 1 cut(s) 67
BstC8I GCNNGC 2 cut(s) 119, 217
BstDEI CTNAG 1 cut(s) 267
BstF5I GGATG 1 cut(s) 381
BstFNI CGCG 1 cut(s) 33
BstHHI GCGC 1 cut(s) 33
BstKTI GATC 2 cut(s) 155, 401
BstMAI GTCTC 1 cut(s) 40
BstMBI GATC 2 cut(s) 152, 398
BstMWI GCNNNNNNNGC 2 cut(s) 254, 463
BstSCI CCNGG 3 cut(s) 361, 373, 392
BstSLI GKGCMC 1 cut(s) 65
BstUI CGCG 1 cut(s) 33
BstV1I GCAGC 3 cut(s) 258, 261, 351
BsuRI GGCC 3 cut(s) 257, 416, 471
BtsCI GGATG 1 cut(s) 381
BtsI GCAGTG 2 cut(s) 65, 255
BtsIMutI CAGTG 3 cut(s) 63, 65, 255
Cac8I GCNNGC 2 cut(s) 119, 217
CfoI GCGC 1 cut(s) 33
CseI GACGC 2 cut(s) 22, 254
Csp6I GTAC 2 cut(s) 68, 422
CviAII CATG 2 cut(s) 136, 467
CviQI GTAC 2 cut(s) 68, 422
DdeI CTNAG 1 cut(s) 267
DpnI GATC 2 cut(s) 154, 400
DpnII GATC 2 cut(s) 152, 398
EaeI YGGCCR 1 cut(s) 255
Eam1104I CTCTTC 1 cut(s) 440
EarI CTCTTC 1 cut(s) 440
Eco130I CCWWGG 1 cut(s) 404
Eco24I GRGCYC 1 cut(s) 375
Eco57I CTGAAG 1 cut(s) 126
EcoT14I CCWWGG 1 cut(s) 404
EcoT38I GRGCYC 1 cut(s) 375
ErhI CCWWGG 1 cut(s) 404
Esp3I CGTCTC 1 cut(s) 40
FaeI CATG 2 cut(s) 139, 470
FaiI YATR 7 cut(s) 98, 137, 206, 308, 387, 446, 468
FaqI GGGAC 1 cut(s) 25
FatI CATG 2 cut(s) 135, 466
FauI CCCGC 1 cut(s) 212
Fnu4HI GCNGC 3 cut(s) 272, 275, 340
FokI GGATG 1 cut(s) 368
FriOI GRGCYC 1 cut(s) 375
Fsp4HI GCNGC 3 cut(s) 272, 275, 340
FspBI CTAG 1 cut(s) 481
GlaI GCGC 1 cut(s) 32
GluI GCNGC 3 cut(s) 272, 275, 340
HaeIII GGCC 3 cut(s) 257, 416, 471
HapII CCGG 3 cut(s) 362, 375, 393
HgaI GACGC 2 cut(s) 22, 254
HhaI GCGC 1 cut(s) 33
Hin1II CATG 2 cut(s) 139, 470
Hin6I GCGC 1 cut(s) 31
HinP1I GCGC 1 cut(s) 31
HincII GTYRAC 1 cut(s) 349
HindII GTYRAC 1 cut(s) 349
HpaI GTTAAC 1 cut(s) 349
HpaII CCGG 3 cut(s) 362, 375, 393
Hpy166II GTNNAC 4 cut(s) 63, 70, 241, 349
Hpy188I TCNGA 3 cut(s) 181, 268, 370
Hpy188III TCNNGA 1 cut(s) 150
Hpy8I GTNNAC 4 cut(s) 63, 70, 241, 349
Hpy99I CGWCG 1 cut(s) 248
HpyAV CCTTC 2 cut(s) 468, 482
HpyCH4III ACNGT 2 cut(s) 67, 262
HpyCH4IV ACGT 1 cut(s) 280
HpyCH4V TGCA 5 cut(s) 58, 63, 117, 277, 466
HpyF10VI GCNNNNNNNGC 2 cut(s) 254, 463
HpyF3I CTNAG 1 cut(s) 267
HpySE526I ACGT 1 cut(s) 280
Hsp92II CATG 2 cut(s) 139, 470
HspAI GCGC 1 cut(s) 31
KspAI GTTAAC 1 cut(s) 349
Kzo9I GATC 2 cut(s) 152, 398
LguI GCTCTTC 1 cut(s) 440
LmnI GCTCC 3 cut(s) 195, 370, 377
LpnPI CCDG 7 cut(s) 98, 163, 321, 375, 388, 406, 467
Lsp1109I GCAGC 3 cut(s) 258, 261, 351
MaeI CTAG 1 cut(s) 481
MaeII ACGT 1 cut(s) 280
MaeIII GTNAC 3 cut(s) 19, 89, 230
MalI GATC 2 cut(s) 154, 400
MboI GATC 2 cut(s) 152, 398
MboII GAAGA 1 cut(s) 427
MhlI GDGCHC 2 cut(s) 65, 375
MlsI TGGCCA 1 cut(s) 257
MluCI AATT 3 cut(s) 39, 184, 291
MluNI TGGCCA 1 cut(s) 257
MmeI TCCRAC 2 cut(s) 159, 348
MnlI CCTC 1 cut(s) 85
Mox20I TGGCCA 1 cut(s) 257
MscI TGGCCA 1 cut(s) 257
MseI TTAA 1 cut(s) 348
Msp20I TGGCCA 1 cut(s) 257
MspI CCGG 3 cut(s) 362, 375, 393
MspR9I CCNGG 3 cut(s) 363, 375, 394
Mva1269I GAATGC 1 cut(s) 357
MvnI CGCG 1 cut(s) 33
MwoI GCNNNNNNNGC 2 cut(s) 254, 463
NciI CCSGG 3 cut(s) 363, 375, 394
NdeII GATC 2 cut(s) 152, 398
NlaIII CATG 2 cut(s) 139, 470
NlaIV GGNNCC 3 cut(s) 191, 372, 379
PciSI GCTCTTC 1 cut(s) 440
PcsI WCGNNNNNNNCGW 1 cut(s) 240
PctI GAATGC 1 cut(s) 357
PkrI GCNGC 3 cut(s) 273, 276, 341
Psp1406I AACGTT 1 cut(s) 280
PspN4I GGNNCC 3 cut(s) 191, 372, 379
RsaI GTAC 2 cut(s) 69, 423
RsaNI GTAC 2 cut(s) 68, 422
SapI GCTCTTC 1 cut(s) 440
SaqAI TTAA 1 cut(s) 348
SatI GCNGC 3 cut(s) 272, 275, 340
Sau3AI GATC 2 cut(s) 152, 398
ScrFI CCNGG 3 cut(s) 363, 375, 394
SduI GDGCHC 2 cut(s) 65, 375
SetI ASST 4 cut(s) 77, 148, 273, 283
Sse9I AATT 3 cut(s) 39, 184, 291
SsiI CCGC 1 cut(s) 219
SspI AATATT 1 cut(s) 210
SspMI CTAG 1 cut(s) 481
StyD4I CCNGG 3 cut(s) 361, 373, 392
StyI CCWWGG 1 cut(s) 404
TaaI ACNGT 2 cut(s) 67, 262
TaiI ACGT 1 cut(s) 283
TasI AATT 3 cut(s) 39, 184, 291
TatI WGTACW 2 cut(s) 67, 421
Tru1I TTAA 1 cut(s) 348
Tru9I TTAA 1 cut(s) 348
TscAI CASTG 3 cut(s) 65, 70, 255
TseI GCWGC 3 cut(s) 271, 274, 339
TspDTI ATGAA 1 cut(s) 311
TspRI CASTG 3 cut(s) 65, 70, 255
VneI GTGCAC 1 cut(s) 61
XspI CTAG 1 cut(s) 481
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.