Rmu_sc0009187.1_g000006

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0009187.1
OG0006538
Physical Location & Seq
Reverse (-)
14322 .. 16394
2073 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0009187.1_g000006.1.cds

Sequence Viewer

Length: 582 bp
atggaaagaagactggtaagagatcatgaacaaaaggaacacgagcaaaaatccgacaccccacggagtcggagaccctccggctccgactctgtgcagcgagtcctgctcagggaagaagagcttgacctgaaacatcatgtcctcgaacatggactcgtagtttatgtaccatgtaaagcccaagatgaagaagaggatggagataaagaagcaaagttggagagggggaaagtaatcccagtaagaatagtagtcgttgtagtacctcgccatgttgaatcacttaactccgagaagggttatgttgtttacgacgctgtaatggccactatatccgagctgctgcgacgtttgctggtgatttcgcctttgcttttagtgattgtggttcattggctctccacaggcagccaggtcaacattgggattccggggtctgagccgggagctatcaacagagctgggggatcaccttggggtgtagctttctttctttacttgcatccatctgtagggttgcagatccatcgggataattccaaagacggtgagaatggtttcatggttaggattcgttga
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko05206 map00270 map01100 map05206
Pfam Domains
Protein Families
BAH Chromo DNA_methylase

Protein Analysis

193

Amino Acids

21.74

Weight (kDa)

6.51

Isoelectric Point (pI)

48.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 2 cut(s) 478, 520
AcoI YGGCCR 1 cut(s) 327
AfaI GTAC 2 cut(s) 171, 267
AfiI CCNNNNNNNGG 2 cut(s) 112, 515
AgsI TTSAA 1 cut(s) 281
AjnI CCWGG 1 cut(s) 414
AluBI AGCT 5 cut(s) 124, 343, 452, 464, 488
AluI AGCT 5 cut(s) 124, 343, 452, 464, 488
Alw26I GTCTC 1 cut(s) 67
AlwI GGATC 2 cut(s) 478, 520
AoxI GGCC 1 cut(s) 327
ApeKI GCWGC 4 cut(s) 97, 343, 346, 411
Asp700I GAANNNNTTC 1 cut(s) 560
AsuC2I CCSGG 2 cut(s) 435, 447
AsuHPI GGTGA 3 cut(s) 373, 465, 563
BalI TGGCCA 1 cut(s) 329
BauI CACGAG 1 cut(s) 41
BbsI GAAGAC 1 cut(s) 16
BbvI GCAGC 4 cut(s) 109, 330, 333, 423
BccI CCATC 3 cut(s) 194, 517, 537
BcgI CGANNNNNNTGC 2 cut(s) 512, 546
BciT130I CCWGG 1 cut(s) 416
BcnI CCSGG 2 cut(s) 435, 447
BcoDI GTCTC 1 cut(s) 67
BfmI CTRYAG 1 cut(s) 513
BisI GCNGC 4 cut(s) 98, 344, 347, 412
BlsI GCNGC 4 cut(s) 99, 345, 348, 413
Bme1390I CCNGG 3 cut(s) 416, 435, 447
BmiI GGNNCC 1 cut(s) 85
BmrFI CCNGG 3 cut(s) 416, 435, 447
BmrI ACTGGG 1 cut(s) 236
BmsI GCATC 1 cut(s) 514
BmuI ACTGGG 1 cut(s) 236
BpiI GAAGAC 1 cut(s) 16
BplI GAGNNNNNCTC 2 cut(s) 93, 125
Bpu10I CCTNAGC 1 cut(s) 110
BpuMI CCSGG 2 cut(s) 435, 447
BsaI GGTCTC 1 cut(s) 67
BsaJI CCNNGG 3 cut(s) 62, 434, 476
Bsc4I CCNNNNNNNGG 2 cut(s) 112, 515
Bse1I ACTGG 2 cut(s) 18, 242
BseBI CCWGG 1 cut(s) 416
BseDI CCNNGG 3 cut(s) 62, 434, 476
BseGI GGATG 2 cut(s) 205, 505
BseLI CCNNNNNNNGG 2 cut(s) 112, 515
BseMII CTCAG 2 cut(s) 124, 432
BseNI ACTGG 2 cut(s) 18, 242
BseXI GCAGC 4 cut(s) 109, 330, 333, 423
BseYI CCCAGC 1 cut(s) 464
BsgI GTGCAG 1 cut(s) 116
BshFI GGCC 1 cut(s) 329
BsiSI CCGG 3 cut(s) 81, 434, 446
BslI CCNNNNNNNGG 2 cut(s) 112, 515
BsmAI GTCTC 1 cut(s) 67
BsnI GGCC 1 cut(s) 329
Bso31I GGTCTC 1 cut(s) 67
Bsp143I GATC 3 cut(s) 22, 470, 525
BspANI GGCC 1 cut(s) 329
BspCNI CTCAG 2 cut(s) 123, 433
BspHI TCATGA 1 cut(s) 25
BspLI GGNNCC 1 cut(s) 85
BspPI GGATC 2 cut(s) 478, 520
BspQI GCTCTTC 1 cut(s) 114
BspTNI GGTCTC 1 cut(s) 67
BsrI ACTGG 2 cut(s) 18, 242
BssECI CCNNGG 3 cut(s) 62, 434, 476
BssMI GATC 3 cut(s) 22, 470, 525
BssSI CACGAG 1 cut(s) 41
BssT1I CCWWGG 1 cut(s) 476
Bst2BI CACGAG 1 cut(s) 41
Bst2UI CCWGG 1 cut(s) 416
Bst4CI ACNGT 1 cut(s) 551
Bst6I CTCTTC 2 cut(s) 114, 189
BstDEI CTNAG 2 cut(s) 110, 441
BstDSI CCRYGG 1 cut(s) 62
BstF5I GGATG 2 cut(s) 205, 505
BstKTI GATC 3 cut(s) 25, 473, 528
BstMAI GTCTC 1 cut(s) 67
BstMBI GATC 3 cut(s) 22, 470, 525
BstMWI GCNNNNNNNGC 3 cut(s) 106, 326, 355
BstNI CCWGG 1 cut(s) 416
BstSCI CCNGG 3 cut(s) 414, 433, 445
BstSFI CTRYAG 1 cut(s) 513
BstV1I GCAGC 4 cut(s) 109, 330, 333, 423
BstV2I GAAGAC 1 cut(s) 16
BstX2I RGATCY 1 cut(s) 525
BstYI RGATCY 1 cut(s) 525
BsuRI GGCC 1 cut(s) 329
BtgI CCRYGG 1 cut(s) 62
BtsCI GGATG 2 cut(s) 205, 505
CciI TCATGA 1 cut(s) 25
CseI GACGC 1 cut(s) 326
Csp6I GTAC 2 cut(s) 170, 266
CviAII CATG 6 cut(s) 26, 140, 152, 174, 275, 565
CviQI GTAC 2 cut(s) 170, 266
DdeI CTNAG 2 cut(s) 110, 441
DpnI GATC 3 cut(s) 24, 472, 527
DpnII GATC 3 cut(s) 22, 470, 525
EaeI YGGCCR 1 cut(s) 327
Eam1104I CTCTTC 2 cut(s) 114, 189
EarI CTCTTC 2 cut(s) 114, 189
Eco130I CCWWGG 1 cut(s) 476
Eco31I GGTCTC 1 cut(s) 67
EcoRII CCWGG 1 cut(s) 414
EcoT14I CCWWGG 1 cut(s) 476
ErhI CCWWGG 1 cut(s) 476
FaeI CATG 6 cut(s) 29, 143, 155, 177, 278, 568
FaiI YATR 9 cut(s) 27, 141, 153, 168, 175, 276, 306, 335, 566
FalI AAGNNNNNCTT 2 cut(s) 108, 140
FatI CATG 6 cut(s) 25, 139, 151, 173, 274, 564
Fnu4HI GCNGC 4 cut(s) 98, 344, 347, 412
FokI GGATG 2 cut(s) 212, 492
Fsp4HI GCNGC 4 cut(s) 98, 344, 347, 412
GluI GCNGC 4 cut(s) 98, 344, 347, 412
GsaI CCCAGC 1 cut(s) 468
HaeIII GGCC 1 cut(s) 329
HapII CCGG 3 cut(s) 81, 434, 446
HgaI GACGC 1 cut(s) 326
Hin1II CATG 6 cut(s) 29, 143, 155, 177, 278, 568
HincII GTYRAC 1 cut(s) 421
HindII GTYRAC 1 cut(s) 421
HinfI GANTC 7 cut(s) 67, 89, 102, 156, 281, 430, 574
HpaII CCGG 3 cut(s) 81, 434, 446
HphI GGTGA 3 cut(s) 373, 465, 563
Hpy166II GTNNAC 2 cut(s) 313, 421
Hpy188I TCNGA 6 cut(s) 55, 72, 88, 295, 340, 442
Hpy188III TCNNGA 2 cut(s) 26, 533
Hpy8I GTNNAC 2 cut(s) 313, 421
Hpy99I CGWCG 2 cut(s) 320, 354
HpyAV CCTTC 1 cut(s) 292
HpyCH4III ACNGT 1 cut(s) 551
HpyCH4IV ACGT 1 cut(s) 352
HpyCH4V TGCA 3 cut(s) 97, 505, 523
HpyF10VI GCNNNNNNNGC 3 cut(s) 106, 326, 355
HpyF3I CTNAG 2 cut(s) 110, 441
HpySE526I ACGT 1 cut(s) 352
Hsp92II CATG 6 cut(s) 29, 143, 155, 177, 278, 568
Kzo9I GATC 3 cut(s) 22, 470, 525
LguI GCTCTTC 1 cut(s) 114
LmnI GCTCC 2 cut(s) 89, 449
Lsp1109I GCAGC 4 cut(s) 109, 330, 333, 423
LweI GCATC 1 cut(s) 514
MaeII ACGT 1 cut(s) 352
MalI GATC 3 cut(s) 24, 472, 527
MboI GATC 3 cut(s) 22, 470, 525
MboII GAAGA 5 cut(s) 21, 128, 131, 203, 206
MflI RGATCY 1 cut(s) 525
MlsI TGGCCA 1 cut(s) 329
MluCI AATT 1 cut(s) 538
MluNI TGGCCA 1 cut(s) 329
MlyI GAGTC 4 cut(s) 76, 83, 111, 150
MmeI TCCRAC 4 cut(s) 50, 78, 111, 201
MnlI CCTC 5 cut(s) 88, 155, 190, 219, 279
Mox20I TGGCCA 1 cut(s) 329
MroXI GAANNNNTTC 1 cut(s) 560
MscI TGGCCA 1 cut(s) 329
MseI TTAA 1 cut(s) 288
Msp20I TGGCCA 1 cut(s) 329
MspI CCGG 3 cut(s) 81, 434, 446
MspR9I CCNGG 3 cut(s) 416, 435, 447
MvaI CCWGG 1 cut(s) 416
MwoI GCNNNNNNNGC 3 cut(s) 106, 326, 355
NciI CCSGG 2 cut(s) 435, 447
NdeII GATC 3 cut(s) 22, 470, 525
NlaIII CATG 6 cut(s) 29, 143, 155, 177, 278, 568
NlaIV GGNNCC 1 cut(s) 85
PagI TCATGA 1 cut(s) 25
PciSI GCTCTTC 1 cut(s) 114
PdmI GAANNNNTTC 1 cut(s) 560
PfeI GAWTC 3 cut(s) 281, 430, 574
PkrI GCNGC 4 cut(s) 99, 345, 348, 413
PleI GAGTC 4 cut(s) 75, 83, 110, 150
PpsI GAGTC 4 cut(s) 75, 83, 110, 150
Psp6I CCWGG 1 cut(s) 414
PspFI CCCAGC 1 cut(s) 464
PspGI CCWGG 1 cut(s) 414
PspN4I GGNNCC 1 cut(s) 85
PsuI RGATCY 1 cut(s) 525
RsaI GTAC 2 cut(s) 171, 267
RsaNI GTAC 2 cut(s) 170, 266
SapI GCTCTTC 1 cut(s) 114
SaqAI TTAA 1 cut(s) 288
SatI GCNGC 4 cut(s) 98, 344, 347, 412
Sau3AI GATC 3 cut(s) 22, 470, 525
SchI GAGTC 4 cut(s) 76, 83, 111, 150
ScrFI CCNGG 3 cut(s) 416, 435, 447
SfaNI GCATC 1 cut(s) 514
SfcI CTRYAG 1 cut(s) 513
Sse9I AATT 1 cut(s) 538
StyD4I CCNGG 3 cut(s) 414, 433, 445
StyI CCWWGG 1 cut(s) 476
TaaI ACNGT 1 cut(s) 551
TaiI ACGT 1 cut(s) 355
TaqI TCGA 1 cut(s) 147
TasI AATT 1 cut(s) 538
TfiI GAWTC 3 cut(s) 281, 430, 574
Tru1I TTAA 1 cut(s) 288
Tru9I TTAA 1 cut(s) 288
TseI GCWGC 4 cut(s) 97, 343, 346, 411
TspDTI ATGAA 4 cut(s) 42, 204, 383, 553
TspGWI ACGGA 1 cut(s) 79
XcmI CCANNNNNNNNNTGG 1 cut(s) 422
XmnI GAANNNNTTC 1 cut(s) 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.