pycom03g00970

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0006538
Physical Location & Seq
Reverse (-)
759913 .. 760389
477 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g00970.1

Sequence Viewer

Length: 477 bp
ATGGGACAGAAGTTAAAGAAGTGCGTGTCTCAATTATTTTTCTTGCTGAGCAGTGCACAGTACACACCTCACTCCACCAGAGTAACTGCAAAAAGGCTTTTCACTCTCTGCACACTTCACTATTTGCTCATGGCTTCAGCTTCCAGATCAAGCTCTTGGCTTTTCAGTCTGTCGGAGAATTGGCTCCCAATGGCTACTATAATACTCGTTTGCGGGTTTGTCGGCTATACTGTTTACGATGCAGTGATGGCCACCGTGTCTGAGCTGCTGCAACGTTTGTTGGTAATTTCTCCTTTACTTATAGTCATTGTAGTTCACTGGCTCTCCACAGGCAGCCAAGTTAACATTGGCATTCCGGGGTCGGAGCCCGGAGCCATCCATAGAGCCGGGGGATCGCCTTGGGGTGTGGCCTTTCTACTATTTCTGCTCTTCTTTCTCATATCTTACCAGCCTTCCTTGCATGGCCTTCTTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko05206 map00270 map01100 map05206
Pfam Domains
Protein Families
BAH Chromo DNA_methylase

Protein Analysis

159

Amino Acids

17.42

Weight (kDa)

9.41

Isoelectric Point (pI)

43.49

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 213
AclI AACGTT 1 cut(s) 274
AclWI GGATC 1 cut(s) 400
AcoI YGGCCR 1 cut(s) 249
AcuI CTGAAG 1 cut(s) 120
AfaI GTAC 1 cut(s) 62
AluBI AGCT 3 cut(s) 140, 153, 265
AluI AGCT 3 cut(s) 140, 153, 265
Alw21I GWGCWC 1 cut(s) 58
Alw26I GTCTC 1 cut(s) 33
Alw44I GTGCAC 1 cut(s) 54
AlwI GGATC 1 cut(s) 400
AoxI GGCC 3 cut(s) 249, 408, 463
ApaLI GTGCAC 1 cut(s) 54
ApeKI GCWGC 3 cut(s) 265, 268, 333
AsuC2I CCSGG 3 cut(s) 357, 369, 388
BaeGI GKGCMC 1 cut(s) 58
BalI TGGCCA 1 cut(s) 251
BanII GRGCYC 1 cut(s) 369
Bbv12I GWGCWC 1 cut(s) 58
BbvI GCAGC 3 cut(s) 252, 255, 345
BccI CCATC 2 cut(s) 241, 383
BcnI CCSGG 3 cut(s) 357, 369, 388
BcoDI GTCTC 1 cut(s) 33
BfaI CTAG 1 cut(s) 475
BisI GCNGC 3 cut(s) 266, 269, 334
BlpI GCTNAGC 1 cut(s) 47
BlsI GCNGC 3 cut(s) 267, 270, 335
Bme1390I CCNGG 3 cut(s) 357, 369, 388
BmiI GGNNCC 3 cut(s) 185, 366, 373
BmrFI CCNGG 3 cut(s) 357, 369, 388
BmsI GCATC 1 cut(s) 229
Bpu1102I GCTNAGC 1 cut(s) 47
BpuMI CCSGG 3 cut(s) 357, 369, 388
BsaJI CCNNGG 3 cut(s) 356, 387, 398
BsaXI ACNNNNNCTCC 2 cut(s) 308, 338
Bse1I ACTGG 1 cut(s) 323
BseDI CCNNGG 3 cut(s) 356, 387, 398
BseGI GGATG 1 cut(s) 375
BseMII CTCAG 2 cut(s) 38, 252
BseNI ACTGG 1 cut(s) 323
BseSI GKGCMC 1 cut(s) 58
BseXI GCAGC 3 cut(s) 252, 255, 345
BsgI GTGCAG 1 cut(s) 94
BshFI GGCC 3 cut(s) 251, 410, 465
BsiHKAI GWGCWC 1 cut(s) 58
BsiSI CCGG 3 cut(s) 356, 369, 387
BslFI GGGAC 1 cut(s) 18
BsmAI GTCTC 1 cut(s) 33
BsmFI GGGAC 1 cut(s) 18
BsmI GAATGC 1 cut(s) 351
BsnI GGCC 3 cut(s) 251, 410, 465
Bsp1286I GDGCHC 2 cut(s) 58, 369
Bsp143I GATC 2 cut(s) 146, 392
Bsp1720I GCTNAGC 1 cut(s) 47
BspACI CCGC 1 cut(s) 213
BspANI GGCC 3 cut(s) 251, 410, 465
BspCNI CTCAG 2 cut(s) 39, 253
BspLI GGNNCC 3 cut(s) 185, 366, 373
BspPI GGATC 1 cut(s) 400
BspQI GCTCTTC 1 cut(s) 434
BsrI ACTGG 1 cut(s) 323
BssECI CCNNGG 3 cut(s) 356, 387, 398
BssMI GATC 2 cut(s) 146, 392
BssT1I CCWWGG 1 cut(s) 398
Bst4CI ACNGT 3 cut(s) 60, 232, 256
Bst6I CTCTTC 1 cut(s) 434
BstDEI CTNAG 2 cut(s) 47, 261
BstF5I GGATG 1 cut(s) 375
BstKTI GATC 2 cut(s) 149, 395
BstMAI GTCTC 1 cut(s) 33
BstMBI GATC 2 cut(s) 146, 392
BstMWI GCNNNNNNNGC 2 cut(s) 248, 457
BstSCI CCNGG 3 cut(s) 355, 367, 386
BstSLI GKGCMC 1 cut(s) 58
BstV1I GCAGC 3 cut(s) 252, 255, 345
BsuRI GGCC 3 cut(s) 251, 410, 465
BtsCI GGATG 1 cut(s) 375
BtsI GCAGTG 2 cut(s) 58, 249
BtsIMutI CAGTG 3 cut(s) 58, 249, 316
Csp6I GTAC 1 cut(s) 61
CviAII CATG 2 cut(s) 130, 461
CviQI GTAC 1 cut(s) 61
DdeI CTNAG 2 cut(s) 47, 261
DpnI GATC 2 cut(s) 148, 394
DpnII GATC 2 cut(s) 146, 392
EaeI YGGCCR 1 cut(s) 249
Eam1104I CTCTTC 1 cut(s) 434
EarI CTCTTC 1 cut(s) 434
Eco130I CCWWGG 1 cut(s) 398
Eco24I GRGCYC 1 cut(s) 369
Eco57I CTGAAG 1 cut(s) 120
EcoT14I CCWWGG 1 cut(s) 398
EcoT38I GRGCYC 1 cut(s) 369
ErhI CCWWGG 1 cut(s) 398
FaeI CATG 2 cut(s) 133, 464
FaiI YATR 7 cut(s) 131, 200, 228, 302, 381, 440, 462
FaqI GGGAC 1 cut(s) 18
FatI CATG 2 cut(s) 129, 460
FauI CCCGC 1 cut(s) 206
Fnu4HI GCNGC 3 cut(s) 266, 269, 334
FokI GGATG 1 cut(s) 362
FriOI GRGCYC 1 cut(s) 369
Fsp4HI GCNGC 3 cut(s) 266, 269, 334
FspBI CTAG 1 cut(s) 475
GluI GCNGC 3 cut(s) 266, 269, 334
HaeIII GGCC 3 cut(s) 251, 410, 465
HapII CCGG 3 cut(s) 356, 369, 387
Hin1II CATG 2 cut(s) 133, 464
HincII GTYRAC 1 cut(s) 343
HindII GTYRAC 1 cut(s) 343
HpaI GTTAAC 1 cut(s) 343
HpaII CCGG 3 cut(s) 356, 369, 387
Hpy166II GTNNAC 5 cut(s) 56, 63, 235, 316, 343
Hpy188I TCNGA 3 cut(s) 175, 262, 364
Hpy188III TCNNGA 1 cut(s) 144
Hpy8I GTNNAC 5 cut(s) 56, 63, 235, 316, 343
HpyAV CCTTC 2 cut(s) 462, 476
HpyCH4III ACNGT 3 cut(s) 60, 232, 256
HpyCH4IV ACGT 1 cut(s) 274
HpyCH4V TGCA 6 cut(s) 56, 89, 111, 242, 271, 460
HpyF10VI GCNNNNNNNGC 2 cut(s) 248, 457
HpyF3I CTNAG 2 cut(s) 47, 261
HpySE526I ACGT 1 cut(s) 274
Hsp92II CATG 2 cut(s) 133, 464
KspAI GTTAAC 1 cut(s) 343
Kzo9I GATC 2 cut(s) 146, 392
LguI GCTCTTC 1 cut(s) 434
LmnI GCTCC 3 cut(s) 189, 364, 371
LpnPI CCDG 8 cut(s) 91, 157, 304, 315, 369, 382, 400, 461
Lsp1109I GCAGC 3 cut(s) 252, 255, 345
LweI GCATC 1 cut(s) 229
MaeI CTAG 1 cut(s) 475
MaeII ACGT 1 cut(s) 274
MaeIII GTNAC 1 cut(s) 82
MalI GATC 2 cut(s) 148, 394
MboI GATC 2 cut(s) 146, 392
MboII GAAGA 1 cut(s) 421
MhlI GDGCHC 2 cut(s) 58, 369
MlsI TGGCCA 1 cut(s) 251
MluCI AATT 3 cut(s) 32, 178, 285
MluNI TGGCCA 1 cut(s) 251
MmeI TCCRAC 2 cut(s) 153, 342
MnlI CCTC 1 cut(s) 78
Mox20I TGGCCA 1 cut(s) 251
MscI TGGCCA 1 cut(s) 251
MseI TTAA 2 cut(s) 14, 342
Msp20I TGGCCA 1 cut(s) 251
MspI CCGG 3 cut(s) 356, 369, 387
MspR9I CCNGG 3 cut(s) 357, 369, 388
Mva1269I GAATGC 1 cut(s) 351
MwoI GCNNNNNNNGC 2 cut(s) 248, 457
NciI CCSGG 3 cut(s) 357, 369, 388
NdeII GATC 2 cut(s) 146, 392
NlaIII CATG 2 cut(s) 133, 464
NlaIV GGNNCC 3 cut(s) 185, 366, 373
PciSI GCTCTTC 1 cut(s) 434
PctI GAATGC 1 cut(s) 351
PkrI GCNGC 3 cut(s) 267, 270, 335
Psp1406I AACGTT 1 cut(s) 274
PspN4I GGNNCC 3 cut(s) 185, 366, 373
RsaI GTAC 1 cut(s) 62
RsaNI GTAC 1 cut(s) 61
SapI GCTCTTC 1 cut(s) 434
SaqAI TTAA 2 cut(s) 14, 342
SatI GCNGC 3 cut(s) 266, 269, 334
Sau3AI GATC 2 cut(s) 146, 392
ScrFI CCNGG 3 cut(s) 357, 369, 388
SduI GDGCHC 2 cut(s) 58, 369
SetI ASST 5 cut(s) 70, 142, 155, 267, 277
SfaNI GCATC 1 cut(s) 229
Sse9I AATT 3 cut(s) 32, 178, 285
SsiI CCGC 1 cut(s) 213
SspMI CTAG 1 cut(s) 475
StyD4I CCNGG 3 cut(s) 355, 367, 386
StyI CCWWGG 1 cut(s) 398
TaaI ACNGT 3 cut(s) 60, 232, 256
TaiI ACGT 1 cut(s) 277
TasI AATT 3 cut(s) 32, 178, 285
TatI WGTACW 1 cut(s) 60
Tru1I TTAA 2 cut(s) 14, 342
Tru9I TTAA 2 cut(s) 14, 342
TscAI CASTG 3 cut(s) 58, 249, 323
TseI GCWGC 3 cut(s) 265, 268, 333
TspRI CASTG 3 cut(s) 58, 249, 323
VneI GTGCAC 1 cut(s) 54
XcmI CCANNNNNNNNNTGG 1 cut(s) 344
XspI CTAG 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.