Rorug05G0476300

DNA (cytosine-5)-methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0006538
Physical Location & Seq
Forward (+)
65547865 .. 65555847
7983 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0476300.1

Sequence Viewer

Length: 3246 bp
ATGTTGTCTGAATTGGGGAGAAGGCCAATGCTTGGAGGTAACGAGGGTTCGTTTGGGGATGAATTTGAGAAGGAGATTGGTATGTTGCTTAGGGAGCAGCGTCGGCAGGAGGCTGATGATCGTGAGAGTGACCTGAATATTTACAGGAGTGGGTCGGCGCCGCCGACTGTGGAGGGTTCTTTGAATGCGGTGGGAGGGTTGTTTGCAGGCGGTGGCGGTGGAGGTGGAGGAGCGGGTGTTGGCGGTGGTGGGGGTGCTGGGTCTGTTCTTTCGGAGTTTCCTGGAGCTAAGAATGGGAATGGGTTTGCGTCGGAGGAGGAGCTGAGGTCTGATCCAGCTTATCTGCAATACTATTACTCGAATGTGAATCTGAATCCGAGGCTTCCGCCTCCGTTGTTGTCGAAGGAGGATTGGAGGTATGCACAGAGGATGAAAGGAGGGAGTTCTGTTTTGGGTGGGATTGGGGACAGGAGGAAAGTGAACAGGGCTGATGATGCTAGTGGTGGGAGATCCATGTTTTCGATGCCGCCTGGTTTTAACTCGAGGAAACAAGAGAGTGAGGTGGAGCCGGACAAAGTGCGTGGCTCAGCGGAGTGGGGTGTTGATGGGCTAATTGGTTTGCCAGGTTTAGGACTTGGCAACAAGCAAAAAAGCCTTGCTGAAATCTTTCAGGATGACTTGGGGCGTACGACTCCTGTTCCTGGGCTTCCTTCTCGTCCAGCCAGCCGTAATGCATTTGATGAAAATGTAGAGGGACTTGGTTCTGCTGAAGCAGACCTGGCTCATTTACGCCGTGACTTGATGGCATCTGATGCTCTACGATCTGGTGCAAATGGCCAAGGCATGTCTGCAGCCCAAAGCATGGGCCCACCTTCTTCATACTCCTATGCTGCAGCTCTAGGTGCTTCCTTGTCAAGAAGCACCACTCCTGATCCTCAAATTGTTGCTAGGGCTCCTAGTCCTTGCCTTACACCTATTGGAGGAGGAAGAGTTAGTGCTTCAGAAAAGAGAGGCATTTCTAGTCCAAGCTCCTTTAATGCTGTCTCATCTGGCATCAATGAGTCTGGAGATCTGGTAGCTGCCTTATCCAGCATGAATTTGTCTTCAAATGGTGTAATAGATGATGAGCACCATTTGCCATCACAGATCAAACAGGATGTTATTGATCACCAGAATTATCTCTTTGGTCTGCAAGGTGCTGATAGTCATGCCAAGCAACTTGCTTACTTAAAGAAGTCTGAATCTGGGAATATACATCTGCCTTCTCCACAGTCGGCAAAAGGTTCGTACTCGGATTTGGGTAAGAGCAATGGGGGTGGGTCAGATCTGAACAACTCATCCCCTGACAGGCAAGTTGAGCTGCAAAAATCTGCTGTTCCTTCTAGTAACTTGTATAAAGGATCATCTGCATCGAATCTTAATGGTGGAGGTGGATTGCATAATCAGTATCAGCAAGTGGATAATGCAAATTCATCATTTTCTAACTATGGTTTAAGTGGCTACTCTATGAATCCAGCCTTGGCATCCATGGTGTCTAGCCAACTTGGTACTGGTAATCTGCCTCCTCTGTTTGAAAATGTTGCTGCAGCATCAGGTATGGTACCTCCTGGAATGGATTCAAGAGTACTTGGAGGAGGTTTGGCTTCTGGACCTAACCTAGCAGCTGCTGCATCTGATTCACATAATCTTGGTAGATTAGGGAGTCCGATTGCGGGGACTGGTCTCCAGGCGCCTTATGTTGATCCTATGTATCTTCAATACTTGAGGACATCAGAGTATGCTGCAGCACAGCTTGCTGCCCTGAATGATCCTTCTGTGGATAGGAACTACTTAGGTAATTCATACATGAATTTACTTGAACTTCAAAAGGCGTATCTTGGAGCCTTGCTGTCACCTCAGAAGTCGCAGTATGGTGTTGGTGTTCCCTTGGGGGGTAAGACTGGTGGTTCAAATCATCATGGTTATTATGGAAATCATGCCTTTGGCATGTCTTATCCAGGAAGTCCCATGGCAAGTCCCGTTATTCCCAACTCTCCAGTTGGCCCTGGTAGTCCAATGAGGCACAATGATCTTAATATGTGTTATCCTTCTGGGATGAGGAATTTGAATTTGGGTGGGAGTGTCATGGGACCATGGCATCTGGATGCTGGTTGTAACATAGATGAGACCTTTGCATCATCACTCTTAGAAGAGTTTAAGAGCAACAAAGCCAAGTCTTTTGAACTTTCGGAAATCGGAGGGCATGTTGTGGAATTCAGTGCGGATCAATACGGGAGTCGGTTTATTCAACAAAAACTTGAAACAGCCACTACGGATGAGAAAAACATGGTTTACCAGGAAATCATGCCTCAAGCTCTTGCTCTAATGACTGATGTGTTTGGTAATTATGTGATTCAAAAGTTTTTTGAGCACGGACTTCCATCTCAAAGAAGGGAATTGGCCAACAAGCTTTTTAGTCATGTGCTGACACTTAGCCTTCAAATGTATGGTTGTCGGGTGATACAGAAGGCAATAGAAGTTGTTGATCTGGACCAGAAGATCAAAATGGTCGAAGAACTTGACGGCCATGTCATGCGCTGTGTGCGTGACCAGAATGGGAACCATGTCATTCAGAAGTGTATTGAATGTGTCCCTGAAGAGGCCATTCGTTTTATAGTCTCAACGTTTTTTGATCAAGTTGTGACCCTTTCCACTCATCCATATGGGTGTCGTGTGATACAGAGAGTGCTTGAGCACTGCAATGACCAGAATACTCAGAGCAAAGTTATGGATGAGATTTTAGGAGCTGTTAGCATGCTGGCACAAGATCAGTATGGCAATTATGTTGTTCAGCATGTACTGGAGCATGGAAAGTCACACGAGCGTTCTGCTATAATAAAGGAATTAGCTGGGAAGATAGTCCAAATGAGTCAACAGAAGTTTGCCTCTAATGTTGTAGAGAAATGCTTAGCTTTTGGTGGTCCTGCTGAAAGGGAATTACTAGTGAATGAGATGCTTGGCACTACTGATGAAAACGAGCCTCTTCAGGCAATGATGAAAGATCAGTTTGCAAACTACGTTGTACAGAAAGTGTTGGAGACTTGTGATGACCAACAACGCGAGCTGATTCTTTCACGAATTAAAGTTCATTTGAATGCATTGAAGAAATACACTTACGGGAAACATATCGTGGCTCGTGTAGAAAAACTTGTTGCTGCTGGGGAAAGGAGAGTTGCTGCTGCTCAGTTGGCTCCTCACCCTGCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko01100 ko05206 map00270 map01100 map05206
Pfam Domains
Protein Families
BAH Chromo DNA_methylase

Protein Analysis

1081

Amino Acids

115.54

Weight (kDa)

5.85

Isoelectric Point (pI)

49.24

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 317 - 743 7.9e-173 Nucleic acid binding protein NABP
PUF PF00806 748 - 782 2.8e-10 Pumilio-family RNA binding repeat
PUF PF00806 786 - 817 1.3e-07 Pumilio-family RNA binding repeat
PUF PF00806 820 - 853 1.8e-09 Pumilio-family RNA binding repeat
PUF_NOP9 PF22493 856 - 937 9.9e-08 NOP9-like PUF repeat domain
PUF PF00806 862 - 886 4e-06 Pumilio-family RNA binding repeat
PUF PF00806 892 - 927 7.4e-13 Pumilio-family RNA binding repeat
PUF PF00806 932 - 962 2.6e-09 Pumilio-family RNA binding repeat
PUF PF00806 966 - 999 4.2e-07 Pumilio-family RNA binding repeat
PUF PF00806 1011 - 1040 2e-08 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 2567
Acc65I GGTACC 1 cut(s) 1600
AccB1I GGYRCC 3 cut(s) 157, 1600, 1729
AccB7I CCANNNNNTGG 2 cut(s) 32, 862
AccBSI CCGCTC 1 cut(s) 233
AccII CGCG 1 cut(s) 3099
AclI AACGTT 1 cut(s) 2663
AclWI GGATC 7 cut(s) 326, 504, 926, 1408, 1736, 1802, 2271
AcoI YGGCCR 3 cut(s) 835, 2439, 2563
AcsI RAATTY 7 cut(s) 62, 1096, 1468, 1849, 2101, 2107, 2252
AcuI CTGAAG 4 cut(s) 789, 984, 2655, 3008
AcyI GRCGYC 2 cut(s) 158, 1730
AfaI GTAC 7 cut(s) 688, 1289, 1549, 1602, 1626, 2838, 3063
AfiI CCNNNNNNNGG 9 cut(s) 32, 629, 701, 862, 980, 1347, 1712, 1931, 2638
AhlI ACTAGT 1 cut(s) 2980
AjuI GAANNNNNNNTTGG 4 cut(s) 1502, 1534, 2093, 2125
AloI GAACNNNNNNTCC 2 cut(s) 1322, 1354
Alw21I GWGCWC 3 cut(s) 1131, 2412, 2736
Alw26I GTCTC 5 cut(s) 1048, 1727, 2159, 2662, 3071
AlwI GGATC 7 cut(s) 326, 504, 926, 1408, 1736, 1802, 2271
AlwNI CAGNNNCTG 1 cut(s) 1667
Ama87I CYCGRG 1 cut(s) 541
AoxI GGCC 7 cut(s) 23, 835, 865, 2041, 2439, 2563, 2640
ApaI GGGCCC 1 cut(s) 869
ApoI RAATTY 7 cut(s) 62, 1096, 1468, 1849, 2101, 2107, 2252
Asp700I GAANNNNTTC 2 cut(s) 666, 1615
Asp718I GGTACC 1 cut(s) 1600
AspLEI GCGC 3 cut(s) 160, 1732, 2577
AspS9I GGNCC 7 cut(s) 865, 866, 1649, 2042, 2129, 2530, 2960
AsuHPI GGTGA 4 cut(s) 1160, 1884, 2509, 3227
AvaI CYCGRG 1 cut(s) 541
AvaII GGWCC 4 cut(s) 1649, 2129, 2530, 2960
BaeGI GKGCMC 1 cut(s) 869
BalI TGGCCA 2 cut(s) 837, 2441
BanI GGYRCC 3 cut(s) 157, 1600, 1729
BanII GRGCYC 2 cut(s) 869, 955
BauI CACGAG 2 cut(s) 2858, 3174
BbsI GAAGAC 1 cut(s) 1095
Bbv12I GWGCWC 3 cut(s) 1131, 2412, 2736
BbvCI CCTCAGC 1 cut(s) 323
BccI CCATC 4 cut(s) 599, 796, 1147, 2428
BceAI ACGGC 3 cut(s) 711, 777, 2578
BcgI CGANNNNNNTGC 4 cut(s) 1266, 1300, 2849, 2883
BclI TGATCA 2 cut(s) 1165, 2671
BcoDI GTCTC 5 cut(s) 1048, 1727, 2159, 2662, 3071
BcuI ACTAGT 1 cut(s) 2980
BfaI CTAG 9 cut(s) 498, 899, 948, 957, 1020, 1383, 1536, 1658, 2981
BfmI CTRYAG 4 cut(s) 849, 891, 1584, 1782
BfoI RGCGCY 2 cut(s) 161, 1733
BglII AGATCT 2 cut(s) 1069, 1324
BlpI GCTNAGC 2 cut(s) 586, 2947
BmcAI AGTACT 1 cut(s) 1626
Bme18I GGWCC 4 cut(s) 1649, 2129, 2530, 2960
BmeT110I CYCGRG 1 cut(s) 541
BmgT120I GGNCC 7 cut(s) 865, 866, 1649, 2042, 2129, 2530, 2960
BpiI GAAGAC 1 cut(s) 1095
BpmI CTGGAG 5 cut(s) 303, 1086, 1709, 2019, 2861
Bpu10I CCTNAGC 2 cut(s) 89, 323
Bpu1102I GCTNAGC 2 cut(s) 586, 2947
BpuEI CTTGAG 3 cut(s) 1783, 2334, 2750
BsaHI GRCGYC 2 cut(s) 158, 1730
BsaI GGTCTC 2 cut(s) 1727, 2159
BsaJI CCNNGG 9 cut(s) 377, 701, 838, 1518, 1527, 1926, 2007, 2044, 2132
Bsc4I CCNNNNNNNGG 9 cut(s) 32, 629, 701, 862, 980, 1347, 1712, 1931, 2638
Bse1I ACTGG 5 cut(s) 1555, 1723, 1945, 2036, 2844
Bse3DI GCAATG 3 cut(s) 1315, 2746, 3036
BseDI CCNNGG 9 cut(s) 377, 701, 838, 1518, 1527, 1926, 2007, 2044, 2132
BseLI CCNNNNNNNGG 9 cut(s) 32, 629, 701, 862, 980, 1347, 1712, 1931, 2638
BseMI GCAATG 3 cut(s) 1315, 2746, 3036
BseMII CTCAG 5 cut(s) 314, 600, 1910, 2768, 3236
BseNI ACTGG 5 cut(s) 1555, 1723, 1945, 2036, 2844
BseRI GAGGAG 7 cut(s) 243, 329, 332, 996, 1554, 1647, 3222
BseSI GKGCMC 1 cut(s) 869
BseYI CCCAGC 3 cut(s) 257, 2888, 3197
Bsh1236I CGCG 1 cut(s) 3099
BshFI GGCC 7 cut(s) 25, 837, 867, 2043, 2441, 2565, 2642
BshNI GGYRCC 3 cut(s) 157, 1600, 1729
BsiHKAI GWGCWC 3 cut(s) 1131, 2412, 2736
BsiHKCI CYCGRG 1 cut(s) 541
BsiSI CCGG 1 cut(s) 569
BsiWI CGTACG 1 cut(s) 686
BslFI GGGAC 7 cut(s) 479, 768, 1729, 1989, 2001, 2142, 2615
BslI CCNNNNNNNGG 9 cut(s) 32, 629, 701, 862, 980, 1347, 1712, 1931, 2638
BsmAI GTCTC 5 cut(s) 1048, 1727, 2159, 2662, 3071
BsmFI GGGAC 7 cut(s) 479, 768, 1729, 1989, 2001, 2142, 2615
BsmI GAATGC 2 cut(s) 190, 3139
BsnI GGCC 7 cut(s) 25, 837, 867, 2043, 2441, 2565, 2642
Bso31I GGTCTC 2 cut(s) 1727, 2159
BsoBI CYCGRG 1 cut(s) 541
Bsp120I GGGCCC 1 cut(s) 865
Bsp1286I GDGCHC 5 cut(s) 869, 955, 1131, 2412, 2736
Bsp1407I TGTACA 1 cut(s) 3061
Bsp1720I GCTNAGC 2 cut(s) 586, 2947
Bsp19I CCATGG 3 cut(s) 1527, 2007, 2132
BspANI GGCC 7 cut(s) 25, 837, 867, 2043, 2441, 2565, 2642
BspCNI CTCAG 5 cut(s) 315, 599, 1909, 2767, 3235
BspFNI CGCG 1 cut(s) 3099
BspMAI CTGCAG 4 cut(s) 853, 895, 1588, 1786
BspPI GGATC 7 cut(s) 326, 504, 926, 1408, 1736, 1802, 2271
BspT107I GGYRCC 3 cut(s) 157, 1600, 1729
BspTNI GGTCTC 2 cut(s) 1727, 2159
BsrBI CCGCTC 1 cut(s) 233
BsrDI GCAATG 3 cut(s) 1315, 2746, 3036
BsrGI TGTACA 1 cut(s) 3061
BsrI ACTGG 5 cut(s) 1555, 1723, 1945, 2036, 2844
BssECI CCNNGG 9 cut(s) 377, 701, 838, 1518, 1527, 1926, 2007, 2044, 2132
BssNI GRCGYC 2 cut(s) 158, 1730
BssSI CACGAG 2 cut(s) 2858, 3174
BssT1I CCWWGG 6 cut(s) 838, 1518, 1527, 1926, 2007, 2132
Bst2BI CACGAG 2 cut(s) 2858, 3174
Bst4CI ACNGT 2 cut(s) 169, 1272
Bst6I CTCTTC 4 cut(s) 982, 2184, 2631, 3027
BstACI GRCGYC 2 cut(s) 158, 1730
BstAPI GCANNNNNTGC 4 cut(s) 812, 1135, 1667, 1793
BstAUI TGTACA 1 cut(s) 3061
BstC8I GCNNGC 6 cut(s) 208, 724, 1794, 2795, 2799, 3101
BstDSI CCRYGG 3 cut(s) 1527, 2007, 2132
BstENI CCTNNNNNAGG 1 cut(s) 978
BstFNI CGCG 1 cut(s) 3099
BstH2I RGCGCY 2 cut(s) 161, 1733
BstHHI GCGC 3 cut(s) 160, 1732, 2577
BstMAI GTCTC 5 cut(s) 1048, 1727, 2159, 2662, 3071
BstNSI RCATGY 5 cut(s) 847, 1990, 2246, 2797, 2837
BstSFI CTRYAG 4 cut(s) 849, 891, 1584, 1782
BstSLI GKGCMC 1 cut(s) 869
BstUI CGCG 1 cut(s) 3099
BstV2I GAAGAC 1 cut(s) 1095
BstX2I RGATCY 3 cut(s) 509, 1069, 1324
BstYI RGATCY 3 cut(s) 509, 1069, 1324
BsuRI GGCC 7 cut(s) 25, 837, 867, 2043, 2441, 2565, 2642
BtgI CCRYGG 3 cut(s) 1527, 2007, 2132
BtsI GCAGTG 1 cut(s) 2734
BtsIMutI CAGTG 2 cut(s) 2263, 2734
Cac8I GCNNGC 6 cut(s) 208, 724, 1794, 2795, 2799, 3101
CaiI CAGNNNCTG 1 cut(s) 1667
CfoI GCGC 3 cut(s) 160, 1732, 2577
Cfr13I GGNCC 7 cut(s) 865, 866, 1649, 2042, 2129, 2530, 2960
CseI GACGC 2 cut(s) 89, 297
Csp6I GTAC 7 cut(s) 687, 1288, 1548, 1601, 1625, 2837, 3062
CspCI CAANNNNNGTGG 4 cut(s) 562, 597, 1297, 1332
CviQI GTAC 7 cut(s) 687, 1288, 1548, 1601, 1625, 2837, 3062
DinI GGCGCC 2 cut(s) 159, 1731
DrdI GACNNNNNNGTC 1 cut(s) 2567
DseDI GACNNNNNNGTC 1 cut(s) 2567
EaeI YGGCCR 3 cut(s) 835, 2439, 2563
Eam1104I CTCTTC 4 cut(s) 982, 2184, 2631, 3027
EarI CTCTTC 4 cut(s) 982, 2184, 2631, 3027
EciI GGCGGA 1 cut(s) 375
Eco130I CCWWGG 6 cut(s) 838, 1518, 1527, 1926, 2007, 2132
Eco24I GRGCYC 2 cut(s) 869, 955
Eco31I GGTCTC 2 cut(s) 1727, 2159
Eco47I GGWCC 4 cut(s) 1649, 2129, 2530, 2960
Eco57I CTGAAG 4 cut(s) 789, 984, 2655, 3008
Eco88I CYCGRG 1 cut(s) 541
EcoNI CCTNNNNNAGG 1 cut(s) 978
EcoRI GAATTC 1 cut(s) 2252
EcoT14I CCWWGG 6 cut(s) 838, 1518, 1527, 1926, 2007, 2132
EcoT22I ATGCAT 2 cut(s) 736, 3139
EcoT38I GRGCYC 2 cut(s) 869, 955
EgeI GGCGCC 2 cut(s) 159, 1731
EheI GGCGCC 2 cut(s) 159, 1731
ErhI CCWWGG 6 cut(s) 838, 1518, 1527, 1926, 2007, 2132
FaqI GGGAC 7 cut(s) 479, 768, 1729, 1989, 2001, 2142, 2615
FauI CCCGC 2 cut(s) 226, 1705
FauNDI CATATG 1 cut(s) 2701
FbaI TGATCA 2 cut(s) 1165, 2671
FriOI GRGCYC 2 cut(s) 869, 955
FspBI CTAG 9 cut(s) 498, 899, 948, 957, 1020, 1383, 1536, 1658, 2981
GlaI GCGC 3 cut(s) 159, 1731, 2576
GsaI CCCAGC 3 cut(s) 261, 2892, 3201
GsuI CTGGAG 5 cut(s) 303, 1086, 1709, 2019, 2861
HaeII RGCGCY 2 cut(s) 161, 1733
HaeIII GGCC 7 cut(s) 25, 837, 867, 2043, 2441, 2565, 2642
HapII CCGG 1 cut(s) 569
HgaI GACGC 2 cut(s) 89, 297
HhaI GCGC 3 cut(s) 160, 1732, 2577
Hin1I GRCGYC 2 cut(s) 158, 1730
Hin6I GCGC 3 cut(s) 158, 1730, 2575
HinP1I GCGC 3 cut(s) 158, 1730, 2575
HincII GTYRAC 1 cut(s) 2912
HindII GTYRAC 1 cut(s) 2912
HindIII AAGCTT 1 cut(s) 2447
HpaII CCGG 1 cut(s) 569
HphI GGTGA 4 cut(s) 1160, 1884, 2509, 3227
Hpy166II GTNNAC 3 cut(s) 481, 2332, 2912
Hpy8I GTNNAC 3 cut(s) 481, 2332, 2912
Hpy99I CGWCG 2 cut(s) 105, 313
HpyCH4III ACNGT 2 cut(s) 169, 1272
HpyCH4IV ACGT 2 cut(s) 2663, 3057
HpySE526I ACGT 2 cut(s) 2663, 3057
Hsp92I GRCGYC 2 cut(s) 158, 1730
HspAI GCGC 3 cut(s) 158, 1730, 2575
KasI GGCGCC 2 cut(s) 157, 1729
KpnI GGTACC 1 cut(s) 1604
Ksp22I TGATCA 2 cut(s) 1165, 2671
MaeI CTAG 9 cut(s) 498, 899, 948, 957, 1020, 1383, 1536, 1658, 2981
MaeII ACGT 2 cut(s) 2663, 3057
MaeIII GTNAC 9 cut(s) 38, 128, 794, 1385, 1890, 2153, 2585, 2680, 2853
MbiI CCGCTC 1 cut(s) 233
MflI RGATCY 3 cut(s) 509, 1069, 1324
MhlI GDGCHC 5 cut(s) 869, 955, 1131, 2412, 2736
MlsI TGGCCA 2 cut(s) 837, 2441
MluNI TGGCCA 2 cut(s) 837, 2441
Mly113I GGCGCC 2 cut(s) 158, 1730
MlyI GAGTC 5 cut(s) 685, 1070, 1711, 2284, 2917
MmeI TCCRAC 2 cut(s) 291, 3054
Mox20I TGGCCA 2 cut(s) 837, 2441
Mph1103I ATGCAT 2 cut(s) 736, 3139
MroXI GAANNNNTTC 2 cut(s) 666, 1615
MscI TGGCCA 2 cut(s) 837, 2441
MseI TTAA 8 cut(s) 537, 1035, 1229, 1419, 1493, 2073, 2196, 3120
MslI CAYNNNNRTG 5 cut(s) 2142, 2700, 2704, 2739, 3132
Msp20I TGGCCA 2 cut(s) 837, 2441
MspA1I CMGCKG 2 cut(s) 590, 1664
MspI CCGG 1 cut(s) 569
Mva1269I GAATGC 2 cut(s) 190, 3139
MvnI CGCG 1 cut(s) 3099
NarI GGCGCC 2 cut(s) 158, 1730
NcoI CCATGG 3 cut(s) 1527, 2007, 2132
NdeI CATATG 1 cut(s) 2701
NmuCI GTSAC 6 cut(s) 128, 794, 1890, 2585, 2680, 2853
NsiI ATGCAT 2 cut(s) 736, 3139
NspI RCATGY 5 cut(s) 847, 1990, 2246, 2797, 2837
PaeI GCATGC 1 cut(s) 2797
PaeR7I CTCGAG 1 cut(s) 541
PcsI WCGNNNNNNNCGW 1 cut(s) 161
PctI GAATGC 2 cut(s) 190, 3139
PdmI GAANNNNTTC 2 cut(s) 666, 1615
PfeI GAWTC 9 cut(s) 367, 373, 1241, 1414, 1510, 1616, 1676, 2392, 3106
Pfl23II CGTACG 1 cut(s) 686
PflMI CCANNNNNTGG 2 cut(s) 32, 862
PfoI TCCNGGA 3 cut(s) 280, 1606, 1996
PleI GAGTC 5 cut(s) 685, 1069, 1710, 2283, 2916
PluTI GGCGCC 2 cut(s) 161, 1733
PpsI GAGTC 5 cut(s) 685, 1069, 1710, 2283, 2916
Psp1406I AACGTT 1 cut(s) 2663
PspFI CCCAGC 3 cut(s) 257, 2888, 3197
PspLI CGTACG 1 cut(s) 686
PspOMI GGGCCC 1 cut(s) 865
PspPI GGNCC 7 cut(s) 865, 866, 1649, 2042, 2129, 2530, 2960
PspXI VCTCGAGB 1 cut(s) 541
PsrI GAACNNNNNNTAC 2 cut(s) 31, 63
PstI CTGCAG 4 cut(s) 853, 895, 1588, 1786
PstNI CAGNNNCTG 1 cut(s) 1667
PsuI RGATCY 3 cut(s) 509, 1069, 1324
PvuII CAGCTG 1 cut(s) 1664
RsaI GTAC 7 cut(s) 688, 1289, 1549, 1602, 1626, 2838, 3063
RsaNI GTAC 7 cut(s) 687, 1288, 1548, 1601, 1625, 2837, 3062
RseI CAYNNNNRTG 5 cut(s) 2142, 2700, 2704, 2739, 3132
SaqAI TTAA 8 cut(s) 537, 1035, 1229, 1419, 1493, 2073, 2196, 3120
Sau96I GGNCC 7 cut(s) 865, 866, 1649, 2042, 2129, 2530, 2960
ScaI AGTACT 1 cut(s) 1626
SchI GAGTC 5 cut(s) 685, 1070, 1711, 2284, 2917
SduI GDGCHC 5 cut(s) 869, 955, 1131, 2412, 2736
SfcI CTRYAG 4 cut(s) 849, 891, 1584, 1782
SfoI GGCGCC 2 cut(s) 159, 1731
Sfr274I CTCGAG 1 cut(s) 541
SinI GGWCC 4 cut(s) 1649, 2129, 2530, 2960
SlaI CTCGAG 1 cut(s) 541
SmiMI CAYNNNNRTG 5 cut(s) 2142, 2700, 2704, 2739, 3132
SmlI CTYRAG 4 cut(s) 541, 1762, 2349, 2729
SmoI CTYRAG 4 cut(s) 541, 1762, 2349, 2729
SpeI ACTAGT 1 cut(s) 2980
SphI GCATGC 1 cut(s) 2797
SspDI GGCGCC 2 cut(s) 157, 1729
SspI AATATT 1 cut(s) 139
SspMI CTAG 9 cut(s) 498, 899, 948, 957, 1020, 1383, 1536, 1658, 2981
StyI CCWWGG 6 cut(s) 838, 1518, 1527, 1926, 2007, 2132
TaaI ACNGT 2 cut(s) 169, 1272
TaiI ACGT 2 cut(s) 2666, 3060
TaqI TCGA 6 cut(s) 359, 401, 521, 542, 1412, 2550
TatI WGTACW 3 cut(s) 1624, 2836, 3061
TauI GCSGC 2 cut(s) 163, 529
TfiI GAWTC 9 cut(s) 367, 373, 1241, 1414, 1510, 1616, 1676, 2392, 3106
Tru1I TTAA 8 cut(s) 537, 1035, 1229, 1419, 1493, 2073, 2196, 3120
Tru9I TTAA 8 cut(s) 537, 1035, 1229, 1419, 1493, 2073, 2196, 3120
TscAI CASTG 2 cut(s) 2263, 2741
TseFI GTSAC 6 cut(s) 128, 794, 1890, 2585, 2680, 2853
Tsp45I GTSAC 6 cut(s) 128, 794, 1890, 2585, 2680, 2853
TspGWI ACGGA 3 cut(s) 381, 2327, 2427
TspRI CASTG 2 cut(s) 2263, 2741
Van91I CCANNNNNTGG 2 cut(s) 32, 862
VpaK11BI GGWCC 4 cut(s) 1649, 2129, 2530, 2960
XagI CCTNNNNNAGG 1 cut(s) 978
XapI RAATTY 7 cut(s) 62, 1096, 1468, 1849, 2101, 2107, 2252
XceI RCATGY 5 cut(s) 847, 1990, 2246, 2797, 2837
XcmI CCANNNNNNNNNTGG 1 cut(s) 1547
XhoI CTCGAG 1 cut(s) 541
XmnI GAANNNNTTC 2 cut(s) 666, 1615
XspI CTAG 9 cut(s) 498, 899, 948, 957, 1020, 1383, 1536, 1658, 2981
ZrmI AGTACT 1 cut(s) 1626
Zsp2I ATGCAT 2 cut(s) 736, 3139
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.