Rw5G049050

Protein kinase domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011667
Physical Location & Seq
Reverse (-)
85078546 .. 85084658
6113 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G049050.1

Sequence Viewer

Length: 2169 bp
ATGCCGGAGCTTCGAAAAGGTGTCCGCCGAGGTCGTGCTAGGGTAGCTCACAAGCCGTCGGACCCCCCGCCGCCGCCGCCTCCGTCTCGAAGGACGCGTGCCACGGTCGCGAGGCAGGTAGCTGAGGCTGTGGTCAGGCCGAGGACGAGGTTGGCCGTGAAAAAGCTGAAGGAGGAGGAGGTGCTGCCGGAGCCGGAGAAGGAGAACGAGAAGGACCGCGTGATTGTGATTTCGGAGAAGGAAGACTCCGATTCGGAGGGTGAAAGGGAGGAAGAAGAAGTAGAAGAAGAGGAGGAGGAGGAGGAGGAGGAGGAAGAAGAAGAGGAAGAAGAAGAAGAAGAAGAGAACAAAGCTGTGATGGCTGACGATAGTGGTGGCTTGAGTGCTAATAAGGCTGCTGGGCAAGAAGAAGAGGGCAGTACGGCGCCGTTTCCTGAAAAGGTTCAAGTAGGAGGATCGCCGCAATATAAGGTGGAAAGGAAGCTTGGCAAAGGTGGCTTTGGTCAGGTGTTCGTTGGTCGTCGTGTTACAGGTGGAGTTGACCGAACAAGTGGTCCTGGTGCTATTGAGGTAGCACTGAAATTCGAGCACAGAAATAGTAAAGGCTGCAGCTATGGTCCTCCATATGAGTGGCAAGTTTACAACACTCTTGGTGGTAGTCATGGAGTTCCCAGAGTACATTATAAAGGAAAGCAAGGGGACTATTATGTAATGGTTATGGACATGCTAGGACCTAGTTTATGGGATGTATGGAATACTTCAGGGCAAGCGATGTCTGCAGAAATGGTAGCTTGTATAGCTGTTGAGTCTTTATCGATTCTGGAGAAGATGCACTCTAGAGGGTATGTGCATGGAGATGTAAAGCCAGAGAACTTTTTGCTTGGTCAGCCATCTACCGCTCAAGAGAAGAAATTGTATCTTGTTGACCTCGGATTAGCAACAAAGTGGAAAGACAGCTCAAGTGGGCTTCATGTTGAATATGATCAACGTCCTGATATGTTTAGAGGAACTGTTCGATATGCTAGTGTTCATGCTCACTTGGGAAGAACTGCTAGTAGACGAGATGACCTCGAATCACTTGCTTATACACTTATTTTTCTCCATCGAGGAAGGTTGCCATGGCAGGGGTATCAGGGTGATAACAAATCTTTCCTAGTTTGTAAAAAGAAGATGGCAACATCACCTGAAATGCTGTGCTGTTTCTGCCCCGCAGCTCTAAGGCAATTTCTTGAGATCGTGGTGAACATGAAATTTGATGAAGAGCCTAACTATTCAAAATTAATATCACTGTTTGAGGGATTGATAGGAACAAATCCTGCTGTAAGACCATTAAAAATTGATGGTGCTCAGAAGATTATTAGCCAGGTTGGCCAGAAACGGGGTAGATTGAATATTGAGGAAGATGATGACGGGCAGCCAAGGAAGAAGGTTCGGCTAGGAGTACCTGCTACACAATGGATTTCAATTTACAATGCTCGGATGCCAATGAAACAAAGGTATCATTACAACGTCGCTGATGCAAGGTTGGCACAACATGTGGAGAGAGGAATTGCAGATGGTCTGCTTATAAGTTGCGTGTCTTCGTGTTCTAATCTCTGGGCACTTATTATGGATGCTGGAACTGGTTTTTCAAACCAAGTTTATGAGCTTTCTCCCTTCTTCTTGCACAAGGAATGGATAATGGAACAATGGGAAAAGAATTATTACATTAGTTCTATTGCTGGTGCTAACAATGGGAGTTCCCTTGTGGTGATGTCCAAAGGAACCCAGTATACTCAACAGTCTTACAAAGTGAGCGATTCTTTCCCCTTCAAGTGGATAAACAAAAAGTGGAGGGAAGGTTTTCATGTAACTTCAATGGCAACTGCTGGGAGCCGGTGGGGTGTTGTAATGTCTCGGAATGCTGGCTTCAGTGATCAGGTTGTGGAGCTTGATTTTCTCTATCCAAGTGAAGGCATCCACAGGCGTTGGGATAATGGTTTCCGGATAACATCAACAGCTGCAACCTGGGATCAAGCGGCTCTTATCTTGAGTGTTCCCAAGCGCAAACCTGGTGATGAAACACAGGAGACCCTGCGTACATCACAATTTCCTAGCACACATGTCAAGGAAAAATGGGCAAAGAATCTGTATCTTGCTTGTTTGTGCTATGGACGTACCGTATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04011 ko04392 map04011 map04392
Pfam Domains
Protein Families
Pkinase

Protein Analysis

722

Amino Acids

81.31

Weight (kDa)

6.23

Isoelectric Point (pI)

56.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 156 - 391 2.5e-17 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 157 - 428 5.3e-07 Protein tyrosine and serine/threonine kinase
Pkinase_fungal PF17667 269 - 366 4.1e-07 Fungal protein kinase
DUF7477 PF24289 455 - 722 1.8e-162 Domain of unknown function (DUF7477)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011667)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13670
fragaria_vesca FvH4_3g44690
malus_domestica MD03G1011500.v1.1 MD11G1014200.v1.1
prunus_persica Prupe.6G011900_v2.0.a1 Prupe.6G011900_v2.0.a1 Prupe.6G011900_v2.0.a1
pyrus_communis pycom03g01040 pycom11g00960
rosa_chinensis RchiOBHm_Chr5g0081361
rosa_laevigata RLG00000036927
rosa_multiflora Rmu_sc0007768.1_g000010 Rmu_sc0009187.1_g000017
rosa_roxburghii Rroxscaffold_1G00002180
rosa_rugosa Rorug05G0476000
rosa_samantha Rh5AG527400 Rh5BG551600 Rh5CG574400
rosa_wichuraiana Rw5G049050

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 684, 1568
Acc36I ACCTGC 2 cut(s) 106, 1453
AccB1I GGYRCC 1 cut(s) 424
AccBSI CCGCTC 1 cut(s) 899
AccI GTMKAC 2 cut(s) 1057, 1772
AccII CGCG 3 cut(s) 97, 110, 219
AccIII TCCGGA 1 cut(s) 1983
AclWI GGATC 2 cut(s) 463, 2019
AcoI YGGCCR 2 cut(s) 153, 1369
AcsI RAATTY 2 cut(s) 581, 1250
AcuI CTGAAG 3 cut(s) 188, 744, 1894
AcyI GRCGYC 1 cut(s) 425
AfaI GTAC 5 cut(s) 421, 678, 1443, 2080, 2158
AfiI CCNNNNNNNGG 4 cut(s) 1124, 1378, 1815, 1952
AflIII ACRYGT 3 cut(s) 95, 1534, 2101
AgsI TTSAA 8 cut(s) 446, 977, 1275, 1390, 1464, 1632, 1813, 1857
AjnI CCWGG 4 cut(s) 556, 1362, 2006, 2050
AjuI GAANNNNNNNTTGG 2 cut(s) 468, 500
AloI GAACNNNNNNTCC 2 cut(s) 538, 570
Alw21I GWGCWC 2 cut(s) 591, 1348
Alw26I GTCTC 3 cut(s) 90, 1899, 2063
AlwI GGATC 2 cut(s) 463, 2019
Aor13HI TCCGGA 1 cut(s) 1983
AoxI GGCC 3 cut(s) 137, 153, 1369
ApeKI GCWGC 7 cut(s) 184, 395, 606, 609, 1211, 1414, 2000
ApoI RAATTY 2 cut(s) 581, 1250
ArsI GACNNNNNNTTYG 2 cut(s) 538, 570
AseI ATTAAT 1 cut(s) 1280
Asp700I GAANNNNTTC 2 cut(s) 441, 1842
AspLEI GCGC 2 cut(s) 427, 2046
AspS9I GGNCC 5 cut(s) 61, 214, 554, 617, 731
AsuHPI GGTGA 6 cut(s) 272, 1148, 1173, 1252, 1762, 2066
AsuII TTCGAA 1 cut(s) 13
AvaII GGWCC 5 cut(s) 61, 214, 554, 617, 731
BaeGI GKGCMC 1 cut(s) 1603
BaeI ACNNNNGTAYC 2 cut(s) 1481, 1514
BalI TGGCCA 1 cut(s) 1371
BanI GGYRCC 1 cut(s) 424
BbsI GAAGAC 2 cut(s) 249, 1572
Bbv12I GWGCWC 2 cut(s) 591, 1348
BbvCI CCTCAGC 1 cut(s) 123
BbvI GCAGC 7 cut(s) 171, 382, 593, 621, 1223, 1426, 1987
BccI CCATC 6 cut(s) 352, 898, 1110, 1165, 1334, 1550
BceAI ACGGC 4 cut(s) 40, 140, 412, 438
BcgI CGANNNNNNTGC 2 cut(s) 1061, 1095
BciT130I CCWGG 4 cut(s) 558, 1364, 2008, 2052
BclI TGATCA 2 cut(s) 982, 1915
BcoDI GTCTC 3 cut(s) 90, 1899, 2063
BfaI CTAG 9 cut(s) 39, 728, 735, 837, 1023, 1053, 1154, 1436, 2094
BfmI CTRYAG 2 cut(s) 607, 777
BfoI RGCGCY 1 cut(s) 428
BfuAI ACCTGC 2 cut(s) 106, 1453
BglI GCCNNNNNGGC 1 cut(s) 1368
Bme1390I CCNGG 4 cut(s) 558, 1364, 2008, 2052
Bme18I GGWCC 5 cut(s) 61, 214, 554, 617, 731
BmgT120I GGNCC 5 cut(s) 61, 214, 554, 617, 731
BmiI GGNNCC 5 cut(s) 63, 192, 426, 1765, 1874
BmrFI CCNGG 4 cut(s) 558, 1364, 2008, 2052
BmrI ACTGGG 1 cut(s) 1762
BmsI GCATC 5 cut(s) 819, 1470, 1507, 1603, 1965
BmuI ACTGGG 1 cut(s) 1762
BpiI GAAGAC 2 cut(s) 249, 1572
BplI GAGNNNNNCTC 2 cut(s) 1053, 1085
BpmI CTGGAG 1 cut(s) 842
Bpu10I CCTNAGC 1 cut(s) 123
Bpu14I TTCGAA 1 cut(s) 13
BpuEI CTTGAG 5 cut(s) 400, 885, 943, 1250, 2050
Bsa29I ATCGAT 1 cut(s) 815
BsaHI GRCGYC 1 cut(s) 425
BsaI GGTCTC 1 cut(s) 2063
BsaJI CCNNGG 7 cut(s) 28, 102, 140, 928, 1118, 1418, 2007
BsaWI WCCGGW 1 cut(s) 1983
BsaXI ACNNNNNCTCC 2 cut(s) 1825, 1855
Bsc4I CCNNNNNNNGG 4 cut(s) 1124, 1378, 1815, 1952
Bse118I RCCGGY 1 cut(s) 1875
Bse1I ACTGG 2 cut(s) 1627, 1768
BseAI TCCGGA 1 cut(s) 1983
BseBI CCWGG 4 cut(s) 558, 1364, 2008, 2052
BseCI ATCGAT 1 cut(s) 815
BseDI CCNNGG 7 cut(s) 28, 102, 140, 928, 1118, 1418, 2007
BseGI GGATG 4 cut(s) 751, 1485, 1618, 1956
BseLI CCNNNNNNNGG 4 cut(s) 1124, 1378, 1815, 1952
BseMII CTCAG 2 cut(s) 114, 1361
BseNI ACTGG 2 cut(s) 1627, 1768
BseRI GAGGAG 9 cut(s) 188, 191, 305, 308, 311, 314, 317, 320, 323
BseSI GKGCMC 1 cut(s) 1603
BseXI GCAGC 7 cut(s) 171, 382, 593, 621, 1223, 1426, 1987
BseYI CCCAGC 2 cut(s) 398, 1868
Bsh1236I CGCG 3 cut(s) 97, 110, 219
Bsh1285I CGRYCG 1 cut(s) 108
BshFI GGCC 3 cut(s) 139, 155, 1371
BshNI GGYRCC 1 cut(s) 424
BshVI ATCGAT 1 cut(s) 815
BsiEI CGRYCG 1 cut(s) 108
BsiHKAI GWGCWC 2 cut(s) 591, 1348
BsiSI CCGG 5 cut(s) 5, 188, 194, 1876, 1984
BslFI GGGAC 1 cut(s) 713
BslI CCNNNNNNNGG 4 cut(s) 1124, 1378, 1815, 1952
BsmAI GTCTC 3 cut(s) 90, 1899, 2063
BsmBI CGTCTC 1 cut(s) 90
BsmFI GGGAC 1 cut(s) 713
BsmI GAATGC 1 cut(s) 1906
BsnI GGCC 3 cut(s) 139, 155, 1371
Bso31I GGTCTC 1 cut(s) 2063
Bsp119I TTCGAA 1 cut(s) 13
Bsp1286I GDGCHC 3 cut(s) 591, 1348, 1603
Bsp13I TCCGGA 1 cut(s) 1983
Bsp143I GATC 5 cut(s) 455, 982, 1233, 1915, 2011
Bsp19I CCATGG 1 cut(s) 1118
Bsp68I TCGCGA 1 cut(s) 110
BspANI GGCC 3 cut(s) 139, 155, 1371
BspCNI CTCAG 2 cut(s) 115, 1360
BspDI ATCGAT 1 cut(s) 815
BspEI TCCGGA 1 cut(s) 1983
BspFNI CGCG 3 cut(s) 97, 110, 219
BspLI GGNNCC 5 cut(s) 63, 192, 426, 1765, 1874
BspMAI CTGCAG 2 cut(s) 611, 781
BspMI ACCTGC 2 cut(s) 106, 1453
BspPI GGATC 2 cut(s) 463, 2019
BspQI GCTCTTC 1 cut(s) 1254
BspT104I TTCGAA 1 cut(s) 13
BspT107I GGYRCC 1 cut(s) 424
BspTNI GGTCTC 1 cut(s) 2063
BsrBI CCGCTC 1 cut(s) 899
BsrFI RCCGGY 1 cut(s) 1875
BsrI ACTGG 2 cut(s) 1627, 1768
BssAI RCCGGY 1 cut(s) 1875
BssECI CCNNGG 7 cut(s) 28, 102, 140, 928, 1118, 1418, 2007
BssMI GATC 5 cut(s) 455, 982, 1233, 1915, 2011
BssNAI GTATAC 1 cut(s) 1773
BssNI GRCGYC 1 cut(s) 425
BssT1I CCWWGG 2 cut(s) 1118, 1418
Bst1107I GTATAC 1 cut(s) 1773
Bst2UI CCWGG 4 cut(s) 558, 1364, 2008, 2052
Bst4CI ACNGT 5 cut(s) 106, 1012, 1290, 1782, 2161
Bst6I CTCTTC 5 cut(s) 282, 315, 336, 405, 1254
BstACI GRCGYC 1 cut(s) 425
BstBI TTCGAA 1 cut(s) 13
BstC8I GCNNGC 3 cut(s) 99, 768, 1906
BstDEI CTNAG 3 cut(s) 123, 1217, 1347
BstDSI CCRYGG 2 cut(s) 102, 1118
BstF5I GGATG 4 cut(s) 751, 1485, 1618, 1956
BstFNI CGCG 3 cut(s) 97, 110, 219
BstH2I RGCGCY 1 cut(s) 428
BstHHI GCGC 2 cut(s) 427, 2046
BstKTI GATC 5 cut(s) 458, 985, 1236, 1918, 2014
BstMAI GTCTC 3 cut(s) 90, 1899, 2063
BstMBI GATC 5 cut(s) 455, 982, 1233, 1915, 2011
BstMCI CGRYCG 1 cut(s) 108
BstNI CCWGG 4 cut(s) 558, 1364, 2008, 2052
BstNSI RCATGY 3 cut(s) 727, 1538, 2105
BstSCI CCNGG 4 cut(s) 556, 1362, 2006, 2050
BstSFI CTRYAG 2 cut(s) 607, 777
BstSLI GKGCMC 1 cut(s) 1603
BstUI CGCG 3 cut(s) 97, 110, 219
BstV1I GCAGC 7 cut(s) 171, 382, 593, 621, 1223, 1426, 1987
BstV2I GAAGAC 2 cut(s) 249, 1572
BstXI CCANNNNNNTGG 1 cut(s) 630
BstZ17I GTATAC 1 cut(s) 1773
Bsu15I ATCGAT 1 cut(s) 815
BsuRI GGCC 3 cut(s) 139, 155, 1371
BsuTUI ATCGAT 1 cut(s) 815
BtgI CCRYGG 2 cut(s) 102, 1118
BtgZI GCGATG 1 cut(s) 785
BtsCI GGATG 4 cut(s) 751, 1485, 1618, 1956
BtsIMutI CAGTG 3 cut(s) 575, 1286, 1918
BtuMI TCGCGA 1 cut(s) 110
BveI ACCTGC 2 cut(s) 106, 1453
Cac8I GCNNGC 3 cut(s) 99, 768, 1906
CfoI GCGC 2 cut(s) 427, 2046
Cfr10I RCCGGY 1 cut(s) 1875
Cfr13I GGNCC 5 cut(s) 61, 214, 554, 617, 731
ClaI ATCGAT 1 cut(s) 815
CseI GACGC 1 cut(s) 103
CsiI ACCWGGT 1 cut(s) 2050
Csp6I GTAC 5 cut(s) 420, 677, 1442, 2079, 2157
CspCI CAANNNNNGTGG 2 cut(s) 1949, 1984
CviQI GTAC 5 cut(s) 420, 677, 1442, 2079, 2157
DdeI CTNAG 3 cut(s) 123, 1217, 1347
DinI GGCGCC 1 cut(s) 426
DpnI GATC 5 cut(s) 457, 984, 1235, 1917, 2013
DpnII GATC 5 cut(s) 455, 982, 1233, 1915, 2011
EaeI YGGCCR 2 cut(s) 153, 1369
Eam1104I CTCTTC 5 cut(s) 282, 315, 336, 405, 1254
EarI CTCTTC 5 cut(s) 282, 315, 336, 405, 1254
EciI GGCGGA 1 cut(s) 14
Eco130I CCWWGG 2 cut(s) 1118, 1418
Eco31I GGTCTC 1 cut(s) 2063
Eco47I GGWCC 5 cut(s) 61, 214, 554, 617, 731
Eco57I CTGAAG 3 cut(s) 188, 744, 1894
EcoO109I RGGNCCY 1 cut(s) 731
EcoRII CCWGG 4 cut(s) 556, 1362, 2006, 2050
EcoT14I CCWWGG 2 cut(s) 1118, 1418
EgeI GGCGCC 1 cut(s) 426
EheI GGCGCC 1 cut(s) 426
ErhI CCWWGG 2 cut(s) 1118, 1418
Esp3I CGTCTC 1 cut(s) 90
FalI AAGNNNNNCTT 2 cut(s) 2007, 2039
FaqI GGGAC 1 cut(s) 713
FauI CCCGC 2 cut(s) 75, 1216
FauNDI CATATG 1 cut(s) 625
FbaI TGATCA 2 cut(s) 982, 1915
FblI GTMKAC 2 cut(s) 1057, 1772
FokI GGATG 4 cut(s) 758, 1492, 1625, 1943
FspBI CTAG 9 cut(s) 39, 728, 735, 837, 1023, 1053, 1154, 1436, 2094
GlaI GCGC 2 cut(s) 426, 2045
GsaI CCCAGC 2 cut(s) 402, 1872
GsuI CTGGAG 1 cut(s) 842
HaeII RGCGCY 1 cut(s) 428
HaeIII GGCC 3 cut(s) 139, 155, 1371
HapII CCGG 5 cut(s) 5, 188, 194, 1876, 1984
HgaI GACGC 1 cut(s) 103
HhaI GCGC 2 cut(s) 427, 2046
Hin1I GRCGYC 1 cut(s) 425
Hin6I GCGC 2 cut(s) 425, 2044
HinP1I GCGC 2 cut(s) 425, 2044
HincII GTYRAC 2 cut(s) 541, 925
HindII GTYRAC 2 cut(s) 541, 925
HindIII AAGCTT 1 cut(s) 482
HinfI GANTC 7 cut(s) 245, 251, 806, 817, 1073, 1799, 2125
HpaII CCGG 5 cut(s) 5, 188, 194, 1876, 1984
HphI GGTGA 6 cut(s) 272, 1148, 1173, 1252, 1762, 2066
Hpy166II GTNNAC 6 cut(s) 541, 640, 925, 1058, 1243, 1773
Hpy188I TCNGA 8 cut(s) 61, 235, 250, 256, 932, 1350, 1479, 1899
Hpy8I GTNNAC 6 cut(s) 541, 640, 925, 1058, 1243, 1773
Hpy99I CGWCG 3 cut(s) 61, 525, 1514
HpyCH4III ACNGT 5 cut(s) 106, 1012, 1290, 1782, 2161
HpyCH4IV ACGT 3 cut(s) 988, 1509, 2155
HpyCH4V TGCA 8 cut(s) 609, 779, 832, 850, 1520, 1553, 1666, 2003
HpyF3I CTNAG 3 cut(s) 123, 1217, 1347
HpySE526I ACGT 3 cut(s) 988, 1509, 2155
Hsp92I GRCGYC 1 cut(s) 425
HspAI GCGC 2 cut(s) 425, 2044
KasI GGCGCC 1 cut(s) 424
Kpn2I TCCGGA 1 cut(s) 1983
Ksp22I TGATCA 2 cut(s) 982, 1915
Kzo9I GATC 5 cut(s) 455, 982, 1233, 1915, 2011
LguI GCTCTTC 1 cut(s) 1254
LmnI GCTCC 4 cut(s) 7, 190, 1872, 1927
Lsp1109I GCAGC 7 cut(s) 171, 382, 593, 621, 1223, 1426, 1987
LweI GCATC 5 cut(s) 819, 1470, 1507, 1603, 1965
MabI ACCWGGT 1 cut(s) 2050
MaeI CTAG 9 cut(s) 39, 728, 735, 837, 1023, 1053, 1154, 1436, 2094
MaeII ACGT 3 cut(s) 988, 1509, 2155
MaeIII GTNAC 2 cut(s) 526, 1849
MalI GATC 5 cut(s) 457, 984, 1235, 1917, 2013
MbiI CCGCTC 1 cut(s) 899
MboI GATC 5 cut(s) 455, 982, 1233, 1915, 2011
MhlI GDGCHC 3 cut(s) 591, 1348, 1603
MlsI TGGCCA 1 cut(s) 1371
MluI ACGCGT 1 cut(s) 95
MluNI TGGCCA 1 cut(s) 1371
Mly113I GGCGCC 1 cut(s) 425
MlyI GAGTC 2 cut(s) 239, 815
MmeI TCCRAC 1 cut(s) 39
Mox20I TGGCCA 1 cut(s) 1371
MroI TCCGGA 1 cut(s) 1983
MroXI GAANNNNTTC 2 cut(s) 441, 1842
MscI TGGCCA 1 cut(s) 1371
MseI TTAA 3 cut(s) 1280, 1331, 2167
MslI CAYNNNNRTG 2 cut(s) 628, 855
Msp20I TGGCCA 1 cut(s) 1371
MspA1I CMGCKG 1 cut(s) 2000
MspI CCGG 5 cut(s) 5, 188, 194, 1876, 1984
MspR9I CCNGG 4 cut(s) 558, 1364, 2008, 2052
Mva1269I GAATGC 1 cut(s) 1906
MvaI CCWGG 4 cut(s) 558, 1364, 2008, 2052
MvnI CGCG 3 cut(s) 97, 110, 219
NarI GGCGCC 1 cut(s) 425
NcoI CCATGG 1 cut(s) 1118
NdeI CATATG 1 cut(s) 625
NdeII GATC 5 cut(s) 455, 982, 1233, 1915, 2011
NlaIV GGNNCC 5 cut(s) 63, 192, 426, 1765, 1874
NmeAIII GCCGAG 2 cut(s) 53, 165
NruI TCGCGA 1 cut(s) 110
NspI RCATGY 3 cut(s) 727, 1538, 2105
NspV TTCGAA 1 cut(s) 13
PciI ACATGT 2 cut(s) 1534, 2101
PciSI GCTCTTC 1 cut(s) 1254
PcsI WCGNNNNNNNCGW 1 cut(s) 94
PctI GAATGC 1 cut(s) 1906
PdmI GAANNNNTTC 2 cut(s) 441, 1842
PfeI GAWTC 5 cut(s) 251, 817, 1073, 1799, 2125
PleI GAGTC 2 cut(s) 239, 814
PluTI GGCGCC 1 cut(s) 428
PpsI GAGTC 2 cut(s) 239, 814
PpuMI RGGWCCY 1 cut(s) 731
PscI ACATGT 2 cut(s) 1534, 2101
PshBI ATTAAT 1 cut(s) 1280
PsiI TTATAA 2 cut(s) 684, 1568
Psp5II RGGWCCY 1 cut(s) 731
Psp6I CCWGG 4 cut(s) 556, 1362, 2006, 2050
PspFI CCCAGC 2 cut(s) 398, 1868
PspGI CCWGG 4 cut(s) 556, 1362, 2006, 2050
PspN4I GGNNCC 5 cut(s) 63, 192, 426, 1765, 1874
PspPI GGNCC 5 cut(s) 61, 214, 554, 617, 731
PspPPI RGGWCCY 1 cut(s) 731
PsrI GAACNNNNNNTAC 2 cut(s) 1756, 1788
PstI CTGCAG 2 cut(s) 611, 781
PvuII CAGCTG 1 cut(s) 2000
RruI TCGCGA 1 cut(s) 110
RsaI GTAC 5 cut(s) 421, 678, 1443, 2080, 2158
RsaNI GTAC 5 cut(s) 420, 677, 1442, 2079, 2157
RseI CAYNNNNRTG 2 cut(s) 628, 855
SapI GCTCTTC 1 cut(s) 1254
SaqAI TTAA 3 cut(s) 1280, 1331, 2167
Sau3AI GATC 5 cut(s) 455, 982, 1233, 1915, 2011
Sau96I GGNCC 5 cut(s) 61, 214, 554, 617, 731
SchI GAGTC 2 cut(s) 239, 815
ScrFI CCNGG 4 cut(s) 558, 1364, 2008, 2052
SduI GDGCHC 3 cut(s) 591, 1348, 1603
SexAI ACCWGGT 1 cut(s) 2050
SfaNI GCATC 5 cut(s) 819, 1470, 1507, 1603, 1965
SfcI CTRYAG 2 cut(s) 607, 777
SfoI GGCGCC 1 cut(s) 426
SfuI TTCGAA 1 cut(s) 13
SinI GGWCC 5 cut(s) 61, 214, 554, 617, 731
SmiMI CAYNNNNRTG 2 cut(s) 628, 855
SmlI CTYRAG 5 cut(s) 379, 900, 958, 1229, 2029
SmoI CTYRAG 5 cut(s) 379, 900, 958, 1229, 2029
SspDI GGCGCC 1 cut(s) 424
SspI AATATT 1 cut(s) 1393
SspMI CTAG 9 cut(s) 39, 728, 735, 837, 1023, 1053, 1154, 1436, 2094
StyD4I CCNGG 4 cut(s) 556, 1362, 2006, 2050
StyI CCWWGG 2 cut(s) 1118, 1418
TaaI ACNGT 5 cut(s) 106, 1012, 1290, 1782, 2161
TaiI ACGT 3 cut(s) 991, 1512, 2158
TaqI TCGA 7 cut(s) 13, 88, 585, 815, 1015, 1071, 1105
TaqII GACCGA 1 cut(s) 558
TatI WGTACW 1 cut(s) 676
TauI GCSGC 5 cut(s) 73, 76, 79, 463, 2021
TfiI GAWTC 5 cut(s) 251, 817, 1073, 1799, 2125
Tru1I TTAA 3 cut(s) 1280, 1331, 2167
Tru9I TTAA 3 cut(s) 1280, 1331, 2167
TscAI CASTG 3 cut(s) 582, 1293, 1918
TseI GCWGC 7 cut(s) 184, 395, 606, 609, 1211, 1414, 2000
TspDTI ATGAA 7 cut(s) 959, 1019, 1262, 1272, 1502, 1835, 2073
TspGWI ACGGA 1 cut(s) 72
TspRI CASTG 3 cut(s) 582, 1293, 1918
VpaK11BI GGWCC 5 cut(s) 61, 214, 554, 617, 731
VspI ATTAAT 1 cut(s) 1280
XapI RAATTY 2 cut(s) 581, 1250
XbaI TCTAGA 1 cut(s) 836
XceI RCATGY 3 cut(s) 727, 1538, 2105
XmiI GTMKAC 2 cut(s) 1057, 1772
XmnI GAANNNNTTC 2 cut(s) 441, 1842
XspI CTAG 9 cut(s) 39, 728, 735, 837, 1023, 1053, 1154, 1436, 2094
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.