Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0008375

Physical Location & Seq

Genomic Coordinates Forward (+)

2798520 .. 2800896

Gene Structure 2377 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1034800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1029 bp

ATGAATAGCCGTTACCTTTTTTCACCAGAACCCATGTGTCAATCTGCTTCCTCTGCGGTTTCATTTTCGTCAAATTCTCTGGGAGATGATCGTGGGGCGGTTAGCCTGGTCAGGACAAGGTCTTCTAGAGCTCCCCCTTCTTCATTCTTAATGAGAATGGCTATGAGAATATCTAGAGCAAGGTGGTTCAACTTCTTGAGAAGAGTCTTCCACTACCAAAATGGTTCGAGGTCCAATCTTGGATCAAATCCTTTCAATAACAGCACTTGGATGATGCTGGAATTCATGGCTTTGGTTGTTCAAATAGGCGTGACCACGATTACTTTGGCTGTTTCGAAGAAGGAGAGGCCGGTTTGGCCTATGAGAATTTGGATTATTGGGTATGATATTGGATGTGTTCTCAATTTGTTCGTGCTCTTTGGGCGTTACAGGCTACTTCATCCGACTCAAAGTGATGGTTTCAACCTCTCCGATTTGGAACAGCAGAGAAGCACTGAAGAGTCCAGGACCACACATTTGATGAACAGGTGTAGAACCTCACTTGACCTCTTCTTTGCGATATGGTTTGTGATGGGTAATGTTTGGGTCTTTGATTCTCGCTTTAGTTCGCTTCCCGGAGCCCCGAAACTCCACGTGCTCTGCATCTCTCTGCTGGCTTGGAATGCCATCAGCTACTCTTTTCCCTTCCTGCTGTTTGTGTTGCTATGTTGCTGTGTACCCCTAATTAGCAGCCTCCTTGGATACAACATGAATATGGGGTCAGTTGATAAAGGAGCATCTGATGACCAAATTTCTCAGCTTCCCAGCTGGAGATACAAAGAAGTTAACGCCAAAGTAGAGCTCGGAAATGATTGCAATGCAGGATCCCTAAATGAAGATCCAGATTGCTGCATTTGCCTGGCCAAATACAGAGACAAGGAGGAAGTAAGGCAGTTGCCATGTTCCCACATGTTTCACCTCAAGTGCGTCGATCAGTGGCTCAGAATCATATCCTGCTGTCCTCTTTGTAAGCAAGAACTGCAGAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_11 zf-RING_2

Protein Analysis

343

Amino Acids

39.08

Weight (kDa)

8.87

Isoelectric Point (pI)

42.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-RING_2	PF13639	295 - 337	2.8e-12	Ring finger domain
zf-RING_11	PF17123	295 - 323	5.7e-11	RING-like zinc finger
zf-rbx1	PF12678	295 - 337	5.7e-06	RING-H2 zinc finger domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0008375)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G55970 AT5G55970 AT5G55970
fragaria_vesca	FvH4_3g42300 FvH4_3g42300
malus_domestica	MD03G1034800.v1.1 MD11G1036700.v1.1
prunus_persica	Prupe.6G028000_v2.0.a1 Prupe.6G028000_v2.0.a1
pyrus_communis	pycom03g02700 pycom11g03040
rosa_chinensis	RchiOBHm_Chr5g0075651
rosa_laevigata	RLG00000036585
rosa_multiflora	Rmu_sc0001764.1_g000001
rosa_roxburghii	Rroxscaffold_1G00005940 Rroxscaffold_3G00219110
rosa_rugosa	Rorug05G0442000 Rorug05G0442100
rosa_samantha	Rh5AG498400 Rh5BG519300 Rh5CG542900 Rh5DG529900
rosa_wichuraiana	Rw5G046220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AciI	CCGC	2 cut(s)	56, 98
AclWI	GGATC	4 cut(s)	250, 858, 871, 872
AcoI	YGGCCR	1 cut(s)	900
AcsI	RAATTY	4 cut(s)	73, 281, 366, 789
AcuI	CTGAAG	1 cut(s)	516
AcvI	CACGTG	1 cut(s)	634
AfaI	GTAC	1 cut(s)	717
AflIII	ACRYGT	1 cut(s)	948
AgsI	TTSAA	4 cut(s)	190, 256, 302, 463
AjnI	CCWGG	3 cut(s)	105, 503, 897
AluBI	AGCT	5 cut(s)	131, 672, 799, 807, 841
AluI	AGCT	5 cut(s)	131, 672, 799, 807, 841
Alw21I	GWGCWC	4 cut(s)	133, 417, 639, 843
Alw26I	GTCTC	1 cut(s)	906
AlwI	GGATC	4 cut(s)	250, 858, 871, 872
AoxI	GGCC	3 cut(s)	347, 356, 900
ApeKI	GCWGC	2 cut(s)	729, 888
ApoI	RAATTY	4 cut(s)	73, 281, 366, 789
ArsI	GACNNNNNNTTYG	4 cut(s)	499, 531, 536, 568
AspS9I	GGNCC	2 cut(s)	231, 507
AsuC2I	CCSGG	1 cut(s)	615
AsuHPI	GGTGA	2 cut(s)	15, 947
AsuII	TTCGAA	1 cut(s)	335
AvaII	GGWCC	2 cut(s)	231, 507
BalI	TGGCCA	1 cut(s)	902
BamHI	GGATCC	1 cut(s)	863
BanII	GRGCYC	3 cut(s)	133, 622, 843
BbrPI	CACGTG	1 cut(s)	634
BbsI	GAAGAC	2 cut(s)	114, 199
Bbv12I	GWGCWC	4 cut(s)	133, 417, 639, 843
BbvI	GCAGC	2 cut(s)	741, 875
BccI	CCATC	3 cut(s)	449, 565, 674
BciT130I	CCWGG	3 cut(s)	107, 505, 899
BciVI	GTATCC	1 cut(s)	734
BcnI	CCSGG	1 cut(s)	615
BcoDI	GTCTC	1 cut(s)	906
BfaI	CTAG	2 cut(s)	126, 174
BfmI	CTRYAG	1 cut(s)	1019
BfuI	GTATCC	1 cut(s)	734
BglI	GCCNNNNNGGC	1 cut(s)	355
BisI	GCNGC	2 cut(s)	730, 889
BlsI	GCNGC	2 cut(s)	731, 890
Bme1390I	CCNGG	4 cut(s)	107, 505, 615, 899
Bme18I	GGWCC	2 cut(s)	231, 507
BmgT120I	GGNCC	2 cut(s)	231, 507
BmiI	GGNNCC	2 cut(s)	619, 865
BmrFI	CCNGG	4 cut(s)	107, 505, 615, 899
BmsI	GCATC	3 cut(s)	264, 651, 785
BpiI	GAAGAC	2 cut(s)	114, 199
BpmI	CTGGAG	1 cut(s)	829
Bpu14I	TTCGAA	1 cut(s)	335
BpuEI	CTTGAG	2 cut(s)	217, 944
BpuMI	CCSGG	1 cut(s)	615
BsaAI	YACGTR	1 cut(s)	634
BsaJI	CCNNGG	1 cut(s)	736
Bse118I	RCCGGY	1 cut(s)	349
Bse3DI	GCAATG	1 cut(s)	862
BseBI	CCWGG	3 cut(s)	107, 505, 899
BseDI	CCNNGG	1 cut(s)	736
BseGI	GGATG	3 cut(s)	276, 398, 439
BseMI	GCAATG	1 cut(s)	862
BseMII	CTCAG	2 cut(s)	809, 994
BseXI	GCAGC	2 cut(s)	741, 875
BseYI	CCCAGC	1 cut(s)	803
BshFI	GGCC	3 cut(s)	349, 358, 902
BsiHKAI	GWGCWC	4 cut(s)	133, 417, 639, 843
BsiSI	CCGG	2 cut(s)	350, 615
BsmAI	GTCTC	1 cut(s)	906
BsmI	GAATGC	1 cut(s)	667
BsnI	GGCC	3 cut(s)	349, 358, 902
Bsp119I	TTCGAA	1 cut(s)	335
Bsp1286I	GDGCHC	5 cut(s)	133, 417, 622, 639, 843
Bsp143I	GATC	5 cut(s)	88, 242, 863, 877, 970
BspACI	CCGC	2 cut(s)	56, 98
BspANI	GGCC	3 cut(s)	349, 358, 902
BspCNI	CTCAG	2 cut(s)	808, 993
BspLI	GGNNCC	2 cut(s)	619, 865
BspMAI	CTGCAG	1 cut(s)	1023
BspPI	GGATC	4 cut(s)	250, 858, 871, 872
BspT104I	TTCGAA	1 cut(s)	335
BsrDI	GCAATG	1 cut(s)	862
BsrFI	RCCGGY	1 cut(s)	349
BssAI	RCCGGY	1 cut(s)	349
BssECI	CCNNGG	1 cut(s)	736
BssMI	GATC	5 cut(s)	88, 242, 863, 877, 970
BssT1I	CCWWGG	1 cut(s)	736
Bst2UI	CCWGG	3 cut(s)	107, 505, 899
Bst6I	CTCTTC	3 cut(s)	196, 492, 554
BstAPI	GCANNNNNTGC	1 cut(s)	1018
BstBAI	YACGTR	1 cut(s)	634
BstBI	TTCGAA	1 cut(s)	335
BstC8I	GCNNGC	1 cut(s)	654
BstDEI	CTNAG	2 cut(s)	795, 980
BstF5I	GGATG	3 cut(s)	276, 398, 439
BstKTI	GATC	5 cut(s)	91, 245, 866, 880, 973
BstMAI	GTCTC	1 cut(s)	906
BstMBI	GATC	5 cut(s)	88, 242, 863, 877, 970
BstMWI	GCNNNNNNNGC	7 cut(s)	53, 355, 421, 430, 662, 894, 1018
BstNI	CCWGG	3 cut(s)	107, 505, 899
BstNSI	RCATGY	1 cut(s)	952
BstSCI	CCNGG	4 cut(s)	105, 503, 613, 897
BstSFI	CTRYAG	1 cut(s)	1019
BstV1I	GCAGC	2 cut(s)	741, 875
BstV2I	GAAGAC	2 cut(s)	114, 199
BstX2I	RGATCY	2 cut(s)	863, 877
BstYI	RGATCY	2 cut(s)	863, 877
BsuI	GTATCC	1 cut(s)	734
BsuRI	GGCC	3 cut(s)	349, 358, 902
BtsCI	GGATG	3 cut(s)	276, 398, 439
BtsIMutI	CAGTG	2 cut(s)	492, 980
Cac8I	GCNNGC	1 cut(s)	654
Cfr10I	RCCGGY	1 cut(s)	349
Cfr13I	GGNCC	2 cut(s)	231, 507
CseI	GACGC	1 cut(s)	955
Csp6I	GTAC	1 cut(s)	716
CviAII	CATG	5 cut(s)	34, 286, 748, 939, 949
CviQI	GTAC	1 cut(s)	716
DdeI	CTNAG	2 cut(s)	795, 980
DpnI	GATC	5 cut(s)	90, 244, 865, 879, 972
DpnII	GATC	5 cut(s)	88, 242, 863, 877, 970
EaeI	YGGCCR	1 cut(s)	900
Eam1104I	CTCTTC	3 cut(s)	196, 492, 554
EarI	CTCTTC	3 cut(s)	196, 492, 554
Ecl136II	GAGCTC	2 cut(s)	131, 841
Eco130I	CCWWGG	1 cut(s)	736
Eco24I	GRGCYC	3 cut(s)	133, 622, 843
Eco47I	GGWCC	2 cut(s)	231, 507
Eco53kI	GAGCTC	2 cut(s)	131, 841
Eco57I	CTGAAG	1 cut(s)	516
Eco72I	CACGTG	1 cut(s)	634
EcoICRI	GAGCTC	2 cut(s)	131, 841
EcoRI	GAATTC	1 cut(s)	281
EcoRII	CCWGG	3 cut(s)	105, 503, 897
EcoT14I	CCWWGG	1 cut(s)	736
EcoT38I	GRGCYC	3 cut(s)	133, 622, 843
ErhI	CCWWGG	1 cut(s)	736
FaeI	CATG	5 cut(s)	37, 289, 751, 942, 952
FatI	CATG	5 cut(s)	33, 285, 747, 938, 948
Fnu4HI	GCNGC	2 cut(s)	730, 889
FokI	GGATG	3 cut(s)	283, 405, 426
FriOI	GRGCYC	3 cut(s)	133, 622, 843
Fsp4HI	GCNGC	2 cut(s)	730, 889
FspBI	CTAG	2 cut(s)	126, 174
GluI	GCNGC	2 cut(s)	730, 889
GsaI	CCCAGC	1 cut(s)	807
GsuI	CTGGAG	1 cut(s)	829
HaeIII	GGCC	3 cut(s)	349, 358, 902
HapII	CCGG	2 cut(s)	350, 615
HgaI	GACGC	1 cut(s)	955
Hin1II	CATG	5 cut(s)	37, 289, 751, 942, 952
HincII	GTYRAC	1 cut(s)	826
HindII	GTYRAC	1 cut(s)	826
HinfI	GANTC	5 cut(s)	204, 445, 500, 593, 984
HpaI	GTTAAC	1 cut(s)	826
HpaII	CCGG	2 cut(s)	350, 615
HphI	GGTGA	2 cut(s)	15, 947
Hpy166II	GTNNAC	2 cut(s)	716, 826
Hpy188I	TCNGA	5 cut(s)	444, 472, 781, 845, 983
Hpy188III	TCNNGA	5 cut(s)	112, 126, 174, 196, 881
Hpy8I	GTNNAC	2 cut(s)	716, 826
Hpy99I	CGWCG	1 cut(s)	971
HpyAV	CCTTC	3 cut(s)	147, 334, 694
HpyCH4IV	ACGT	1 cut(s)	633
HpyCH4V	TGCA	5 cut(s)	642, 855, 860, 891, 1021
HpyF10VI	GCNNNNNNNGC	7 cut(s)	53, 355, 421, 430, 662, 894, 1018
HpyF3I	CTNAG	2 cut(s)	795, 980
HpySE526I	ACGT	1 cut(s)	633
Hsp92II	CATG	5 cut(s)	37, 289, 751, 942, 952
KspAI	GTTAAC	1 cut(s)	826
Kzo9I	GATC	5 cut(s)	88, 242, 863, 877, 970
LmnI	GCTCC	3 cut(s)	136, 617, 773
Lsp1109I	GCAGC	2 cut(s)	741, 875
LweI	GCATC	3 cut(s)	264, 651, 785
MaeI	CTAG	2 cut(s)	126, 174
MaeII	ACGT	1 cut(s)	633
MaeIII	GTNAC	3 cut(s)	11, 310, 425
MalI	GATC	5 cut(s)	90, 244, 865, 879, 972
MboI	GATC	5 cut(s)	88, 242, 863, 877, 970
MboII	GAAGA	8 cut(s)	114, 132, 199, 213, 349, 509, 541, 887
MflI	RGATCY	2 cut(s)	863, 877
MhlI	GDGCHC	5 cut(s)	133, 417, 622, 639, 843
MlsI	TGGCCA	1 cut(s)	902
MluCI	AATT	6 cut(s)	73, 281, 366, 403, 723, 789
MluNI	TGGCCA	1 cut(s)	902
MlyI	GAGTC	3 cut(s)	213, 439, 509
MmeI	TCCRAC	1 cut(s)	467
Mox20I	TGGCCA	1 cut(s)	902
MscI	TGGCCA	1 cut(s)	902
MseI	TTAA	2 cut(s)	149, 825
MslI	CAYNNNNRTG	2 cut(s)	269, 752
Msp20I	TGGCCA	1 cut(s)	902
MspA1I	CMGCKG	1 cut(s)	807
MspI	CCGG	2 cut(s)	350, 615
MspR9I	CCNGG	4 cut(s)	107, 505, 615, 899
Mva1269I	GAATGC	1 cut(s)	667
MvaI	CCWGG	3 cut(s)	107, 505, 899
MwoI	GCNNNNNNNGC	7 cut(s)	53, 355, 421, 430, 662, 894, 1018
NciI	CCSGG	1 cut(s)	615
NdeII	GATC	5 cut(s)	88, 242, 863, 877, 970
NlaIII	CATG	5 cut(s)	37, 289, 751, 942, 952
NlaIV	GGNNCC	2 cut(s)	619, 865
NmuCI	GTSAC	1 cut(s)	310
NspI	RCATGY	1 cut(s)	952
NspV	TTCGAA	1 cut(s)	335
PciI	ACATGT	1 cut(s)	948
PctI	GAATGC	1 cut(s)	667
PfeI	GAWTC	2 cut(s)	593, 984
PflFI	GACNNNGTC	1 cut(s)	118
PfoI	TCCNGGA	2 cut(s)	503, 613
PkrI	GCNGC	2 cut(s)	731, 890
PleI	GAGTC	3 cut(s)	212, 439, 508
PmaCI	CACGTG	1 cut(s)	634
PmlI	CACGTG	1 cut(s)	634
PpsI	GAGTC	3 cut(s)	212, 439, 508
Ppu21I	YACGTR	1 cut(s)	634
PscI	ACATGT	1 cut(s)	948
Psp124BI	GAGCTC	2 cut(s)	133, 843
Psp6I	CCWGG	3 cut(s)	105, 503, 897
PspCI	CACGTG	1 cut(s)	634
PspFI	CCCAGC	1 cut(s)	803
PspGI	CCWGG	3 cut(s)	105, 503, 897
PspN4I	GGNNCC	2 cut(s)	619, 865
PspPI	GGNCC	2 cut(s)	231, 507
PstI	CTGCAG	1 cut(s)	1023
PsuI	RGATCY	2 cut(s)	863, 877
PsyI	GACNNNGTC	1 cut(s)	118
PvuII	CAGCTG	1 cut(s)	807
RsaI	GTAC	1 cut(s)	717
RsaNI	GTAC	1 cut(s)	716
RseI	CAYNNNNRTG	2 cut(s)	269, 752
SacI	GAGCTC	2 cut(s)	133, 843
SaqAI	TTAA	2 cut(s)	149, 825
SatI	GCNGC	2 cut(s)	730, 889
Sau3AI	GATC	5 cut(s)	88, 242, 863, 877, 970
Sau96I	GGNCC	2 cut(s)	231, 507
SchI	GAGTC	3 cut(s)	213, 439, 509
ScrFI	CCNGG	4 cut(s)	107, 505, 615, 899
SduI	GDGCHC	5 cut(s)	133, 417, 622, 639, 843
SfaNI	GCATC	3 cut(s)	264, 651, 785
SfcI	CTRYAG	1 cut(s)	1019
SfiI	GGCCNNNNNGGCC	1 cut(s)	355
SfuI	TTCGAA	1 cut(s)	335
SinI	GGWCC	2 cut(s)	231, 507
SmiMI	CAYNNNNRTG	2 cut(s)	269, 752
SmlI	CTYRAG	2 cut(s)	196, 959
SmoI	CTYRAG	2 cut(s)	196, 959
Sse9I	AATT	6 cut(s)	73, 281, 366, 403, 723, 789
SsiI	CCGC	2 cut(s)	56, 98
SspMI	CTAG	2 cut(s)	126, 174
SstI	GAGCTC	2 cut(s)	133, 843
StyD4I	CCNGG	4 cut(s)	105, 503, 613, 897
StyI	CCWWGG	1 cut(s)	736
TaiI	ACGT	1 cut(s)	636
TaqI	TCGA	3 cut(s)	227, 335, 969
TasI	AATT	6 cut(s)	73, 281, 366, 403, 723, 789
TfiI	GAWTC	2 cut(s)	593, 984
Tru1I	TTAA	2 cut(s)	149, 825
Tru9I	TTAA	2 cut(s)	149, 825
TscAI	CASTG	2 cut(s)	499, 980
TseFI	GTSAC	1 cut(s)	310
TseI	GCWGC	2 cut(s)	729, 888
Tsp45I	GTSAC	1 cut(s)	310
TspDTI	ATGAA	8 cut(s)	17, 51, 132, 274, 428, 536, 764, 888
TspRI	CASTG	2 cut(s)	499, 980
Tth111I	GACNNNGTC	1 cut(s)	118
VpaK11BI	GGWCC	2 cut(s)	231, 507
XapI	RAATTY	4 cut(s)	73, 281, 366, 789
XbaI	TCTAGA	2 cut(s)	125, 173
XceI	RCATGY	1 cut(s)	952
XcmI	CCANNNNNNNNNTGG	2 cut(s)	218, 322
XspI	CTAG	2 cut(s)	126, 174

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.