Rorug05G0442000

E3 ubiquitin-protein ligase At4g11680-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0008375
Physical Location & Seq
Reverse (-)
61406696 .. 61407568
873 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0442000.1

Sequence Viewer

Length: 657 bp
ATGGAGGAAACGAGAAGGGCTGATCACCGAATTGAGCCAGGGAAGCTTACTGTTAGAGACCCACCTTCAAAGCCCAATGTAGTCACTGATCTGGAGGATGCCTTGAAGGTGTTCGATCAAATGCTTCAAAGGCGTCCTCTCCTTTCCATTATCCGTTTCACTCAACTATTGGGTCAACTCATGAAATTGAAGCATTATTCGGAAGCAATATCTTTGTATGATCAAATGCGAGTGTTGCGAATTGCCCCTACTCATTATACTCTCACTATCATCATTAACTGCTATTCTCATTTGAACCATATGGGGTTCAATCTCTCTGTCTTGGCACAATTCTTCAAATTTGGTCTTGAACCAAATGTCACCACCTTCACCACTTTGATCAATGGCTTCCTTCTCTGCCATAGAGTTGCCGAGGCTATACAAATTTTTAGAAAAATGGTGGAGGGAGGTACTTGTAAGCCCGATGTGTTTACTTTTGGCACATTGATGAAGGGCTTGTGCATCAAGGGCAACAATACTGCAGCTATTCAATTGCTCAGGAAGATGGAACAATCTCCAGATTGTAAGCCCAACTTAGTTGTCTATAGCACCATCATTGACAGCCTTTGTAAGGATACACTTATACATACCGCCTATATTACATACCGCCAAGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_11 zf-RING_2

Protein Analysis

218

Amino Acids

24.95

Weight (kDa)

9.15

Isoelectric Point (pI)

34.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PPR_2 PF13041 53 - 96 9e-08 PPR repeat family
PPR_3 PF13812 114 - 164 4.8e-06 Pentatricopeptide repeat domain
PPR_1 PF12854 115 - 147 1.1e-08 PPR repeat
PPR_2 PF13041 118 - 167 7.3e-13 PPR repeat family
PPR_3 PF13812 142 - 200 5.2e-07 Pentatricopeptide repeat domain
PPR_1 PF12854 152 - 183 1.9e-09 PPR repeat
PPR_2 PF13041 154 - 204 3.3e-11 PPR repeat family
PPR_1 PF12854 188 - 212 2.3e-06 PPR repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 630, 646
AcsI RAATTY 2 cut(s) 338, 423
AcyI GRCGYC 1 cut(s) 133
AfaI GTAC 1 cut(s) 451
AfiI CCNNNNNNNGG 1 cut(s) 610
AgsI TTSAA 9 cut(s) 69, 106, 128, 190, 295, 310, 337, 350, 530
AjnI CCWGG 1 cut(s) 37
AluBI AGCT 2 cut(s) 46, 524
AluI AGCT 2 cut(s) 46, 524
Alw26I GTCTC 1 cut(s) 51
ApeKI GCWGC 1 cut(s) 521
ApoI RAATTY 2 cut(s) 338, 423
Asp700I GAANNNNTTC 1 cut(s) 110
AsuHPI GGTGA 3 cut(s) 17, 352, 361
BbvI GCAGC 1 cut(s) 533
BccI CCATC 2 cut(s) 538, 599
BciT130I CCWGG 1 cut(s) 39
BciVI GTATCC 1 cut(s) 607
BclI TGATCA 3 cut(s) 22, 220, 378
BcoDI GTCTC 1 cut(s) 51
BfmI CTRYAG 2 cut(s) 519, 583
BfuI GTATCC 1 cut(s) 607
BisI GCNGC 1 cut(s) 522
BlsI GCNGC 1 cut(s) 523
Bme1390I CCNGG 1 cut(s) 39
BmrFI CCNGG 1 cut(s) 39
BmsI GCATC 2 cut(s) 88, 510
BpmI CTGGAG 2 cut(s) 113, 540
Bpu10I CCTNAGC 1 cut(s) 536
BsaHI GRCGYC 1 cut(s) 133
BsaI GGTCTC 1 cut(s) 51
BsaJI CCNNGG 2 cut(s) 38, 411
Bsc4I CCNNNNNNNGG 1 cut(s) 610
BseBI CCWGG 1 cut(s) 39
BseDI CCNNGG 2 cut(s) 38, 411
BseGI GGATG 1 cut(s) 103
BseLI CCNNNNNNNGG 1 cut(s) 610
BseMII CTCAG 1 cut(s) 550
BseXI GCAGC 1 cut(s) 533
BslI CCNNNNNNNGG 1 cut(s) 610
BsmAI GTCTC 1 cut(s) 51
Bso31I GGTCTC 1 cut(s) 51
Bsp143I GATC 5 cut(s) 22, 88, 115, 220, 378
BspACI CCGC 2 cut(s) 630, 646
BspCNI CTCAG 1 cut(s) 549
BspHI TCATGA 1 cut(s) 180
BspMAI CTGCAG 1 cut(s) 523
BspTNI GGTCTC 1 cut(s) 51
BssECI CCNNGG 2 cut(s) 38, 411
BssMI GATC 5 cut(s) 22, 88, 115, 220, 378
BssNI GRCGYC 1 cut(s) 133
Bst2UI CCWGG 1 cut(s) 39
Bst4CI ACNGT 1 cut(s) 52
BstACI GRCGYC 1 cut(s) 133
BstDEI CTNAG 2 cut(s) 536, 574
BstENI CCTNNNNNAGG 1 cut(s) 608
BstF5I GGATG 1 cut(s) 103
BstKTI GATC 5 cut(s) 25, 91, 118, 223, 381
BstMAI GTCTC 1 cut(s) 51
BstMBI GATC 5 cut(s) 22, 88, 115, 220, 378
BstMWI GCNNNNNNNGC 4 cut(s) 43, 130, 235, 507
BstNI CCWGG 1 cut(s) 39
BstSCI CCNGG 1 cut(s) 37
BstSFI CTRYAG 2 cut(s) 519, 583
BstV1I GCAGC 1 cut(s) 533
BsuI GTATCC 1 cut(s) 607
BtsCI GGATG 1 cut(s) 103
BtsIMutI CAGTG 1 cut(s) 84
CciI TCATGA 1 cut(s) 180
CseI GACGC 1 cut(s) 122
Csp6I GTAC 1 cut(s) 450
CviAII CATG 1 cut(s) 181
CviQI GTAC 1 cut(s) 450
DdeI CTNAG 2 cut(s) 536, 574
DpnI GATC 5 cut(s) 24, 90, 117, 222, 380
DpnII GATC 5 cut(s) 22, 88, 115, 220, 378
Eco31I GGTCTC 1 cut(s) 51
EcoNI CCTNNNNNAGG 1 cut(s) 608
EcoRII CCWGG 1 cut(s) 37
FaeI CATG 1 cut(s) 184
FalI AAGNNNNNCTT 2 cut(s) 557, 589
FatI CATG 1 cut(s) 180
FauNDI CATATG 1 cut(s) 300
FbaI TGATCA 3 cut(s) 22, 220, 378
Fnu4HI GCNGC 1 cut(s) 522
FokI GGATG 1 cut(s) 110
Fsp4HI GCNGC 1 cut(s) 522
GluI GCNGC 1 cut(s) 522
GsuI CTGGAG 2 cut(s) 113, 540
HgaI GACGC 1 cut(s) 122
Hin1I GRCGYC 1 cut(s) 133
Hin1II CATG 1 cut(s) 184
HincII GTYRAC 1 cut(s) 176
HindII GTYRAC 1 cut(s) 176
HindIII AAGCTT 1 cut(s) 44
HphI GGTGA 3 cut(s) 17, 352, 361
Hpy166II GTNNAC 2 cut(s) 176, 471
Hpy188I TCNGA 1 cut(s) 202
Hpy188III TCNNGA 5 cut(s) 92, 181, 347, 538, 557
Hpy8I GTNNAC 2 cut(s) 176, 471
HpyAV CCTTC 6 cut(s) 9, 75, 100, 376, 401, 484
HpyCH4III ACNGT 1 cut(s) 52
HpyCH4V TGCA 2 cut(s) 501, 521
HpyF10VI GCNNNNNNNGC 4 cut(s) 43, 130, 235, 507
HpyF3I CTNAG 2 cut(s) 536, 574
Hsp92I GRCGYC 1 cut(s) 133
Hsp92II CATG 1 cut(s) 184
Ksp22I TGATCA 3 cut(s) 22, 220, 378
Kzo9I GATC 5 cut(s) 22, 88, 115, 220, 378
LpnPI CCDG 5 cut(s) 24, 51, 77, 523, 570
Lsp1109I GCAGC 1 cut(s) 533
LweI GCATC 2 cut(s) 88, 510
MaeIII GTNAC 2 cut(s) 82, 358
MalI GATC 5 cut(s) 24, 90, 117, 222, 380
MboI GATC 5 cut(s) 22, 88, 115, 220, 378
MboII GAAGA 2 cut(s) 325, 553
MfeI CAATTG 1 cut(s) 530
MluCI AATT 7 cut(s) 30, 185, 240, 329, 338, 423, 530
MnlI CCTC 5 cut(s) 88, 147, 406, 436, 440
MroXI GAANNNNTTC 1 cut(s) 110
MseI TTAA 1 cut(s) 276
MslI CAYNNNNRTG 1 cut(s) 485
MspR9I CCNGG 1 cut(s) 39
MunI CAATTG 1 cut(s) 530
MvaI CCWGG 1 cut(s) 39
MwoI GCNNNNNNNGC 4 cut(s) 43, 130, 235, 507
NdeI CATATG 1 cut(s) 300
NdeII GATC 5 cut(s) 22, 88, 115, 220, 378
NlaIII CATG 1 cut(s) 184
NmeAIII GCCGAG 1 cut(s) 436
NmuCI GTSAC 2 cut(s) 82, 358
PagI TCATGA 1 cut(s) 180
PdmI GAANNNNTTC 1 cut(s) 110
PkrI GCNGC 1 cut(s) 523
Psp6I CCWGG 1 cut(s) 37
PspGI CCWGG 1 cut(s) 37
PstI CTGCAG 1 cut(s) 523
RsaI GTAC 1 cut(s) 451
RsaNI GTAC 1 cut(s) 450
RseI CAYNNNNRTG 1 cut(s) 485
SaqAI TTAA 1 cut(s) 276
SatI GCNGC 1 cut(s) 522
Sau3AI GATC 5 cut(s) 22, 88, 115, 220, 378
ScrFI CCNGG 1 cut(s) 39
SetI ASST 6 cut(s) 48, 67, 111, 368, 451, 526
SfaNI GCATC 2 cut(s) 88, 510
SfcI CTRYAG 2 cut(s) 519, 583
SmiMI CAYNNNNRTG 1 cut(s) 485
Sse9I AATT 7 cut(s) 30, 185, 240, 329, 338, 423, 530
SsiI CCGC 2 cut(s) 630, 646
StyD4I CCNGG 1 cut(s) 37
TaaI ACNGT 1 cut(s) 52
TaqI TCGA 1 cut(s) 114
TasI AATT 7 cut(s) 30, 185, 240, 329, 338, 423, 530
Tru1I TTAA 1 cut(s) 276
Tru9I TTAA 1 cut(s) 276
TscAI CASTG 1 cut(s) 91
TseFI GTSAC 2 cut(s) 82, 358
TseI GCWGC 1 cut(s) 521
Tsp45I GTSAC 2 cut(s) 82, 358
TspDTI ATGAA 2 cut(s) 197, 503
TspGWI ACGGA 1 cut(s) 143
TspRI CASTG 1 cut(s) 91
XagI CCTNNNNNAGG 1 cut(s) 608
XapI RAATTY 2 cut(s) 338, 423
XmnI GAANNNNTTC 1 cut(s) 110
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.