Rorug05G0442100

E3 ubiquitin-protein ligase At4g11680-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0008375
Physical Location & Seq
Reverse (-)
61410378 .. 61411300
923 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0442100.1

Sequence Viewer

Length: 792 bp
ATGGCATTTCCAAAGCTCAAGCTTATGATCACCCTTGAGCTGAATTCAACAATTGACAATGTCCCCATTAACCCAAATGTTGAGTTCCACTTCCTTCTCTCCTTCGCTATCGACTACGACACTTCAGGCTCTTCACCAACAAATGGAAATTTCAATGTCTTTTGGGACACCGACAACCTCGGCTCCTCTCAAGTTTCGGGCATCAAGAATGCACATTCAAATGTGAAAGTAGGCTTAAGCTTAGGAGGGGACAGTGTCAATAGTGGCTCTTGTTACTTCAACCCTTCCTCAGTTGATTCATGGGTTACTAATGCTGTTTCTTCACTCACAAGTATCATCCAGCAGTACAATCTAGATGGGATTGACATTGATTACGAGCACTTTCAATCCGATCCCGATACCTTTTCCGAGTGCATTGGAAGACTTATAAAAACCCTCAAGGACAATGGTGTCATCAAGTTTGCTTCCATTGCTCCGTTTGATGACGATGACGTCCAGAGCCACTACTTGGTTTTGTGGAAGAACTATGGCCAAGCACCACAGTGTCTCGATCAGTTCATCGATTATTTCAAGACTCAAAGCTCAAACTACAATGGCGGGAAGGTCTTAGCAAGCTTTAGCACCGATGGGAGTGGTGGATTGTCCCCTGAAAATGTGTTCTTCACTGCCTGCCATAGGCTCAAGAGTGAACAAAACCTTCATGGTATCTTTGTTTGGTCTGCTGATGATTCCAAGAAAAATGGTTTTCGCTATGAAAAGCAATCCCAAGATCTTTTGGCAATTCCACACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_11 zf-RING_2

Protein Analysis

263

Amino Acids

29.07

Weight (kDa)

4.74

Isoelectric Point (pI)

32.37

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_18 PF00704 57 - 149 9.2e-11 Glycosyl hydrolases family 18
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 428
AasI GACNNNNNNGTC 2 cut(s) 449, 491
AatII GACGTC 1 cut(s) 495
AccB7I CCANNNNNTGG 2 cut(s) 143, 508
AciI CCGC 1 cut(s) 597
AclWI GGATC 1 cut(s) 386
AcoI YGGCCR 1 cut(s) 529
AcsI RAATTY 2 cut(s) 43, 148
AcuI CTGAAG 1 cut(s) 108
AcyI GRCGYC 1 cut(s) 492
AfaI GTAC 1 cut(s) 347
AfiI CCNNNNNNNGG 3 cut(s) 143, 508, 675
AflII CTTAAG 1 cut(s) 235
AgsI TTSAA 6 cut(s) 48, 154, 219, 280, 386, 571
AleI CACNNNNGTG 1 cut(s) 541
AluBI AGCT 6 cut(s) 16, 22, 40, 240, 582, 615
AluI AGCT 6 cut(s) 16, 22, 40, 240, 582, 615
Alw21I GWGCWC 1 cut(s) 381
Alw26I GTCTC 1 cut(s) 551
AlwI GGATC 1 cut(s) 386
AoxI GGCC 1 cut(s) 529
ApoI RAATTY 2 cut(s) 43, 148
AsuHPI GGTGA 2 cut(s) 22, 126
BalI TGGCCA 1 cut(s) 531
BbsI GAAGAC 1 cut(s) 427
Bbv12I GWGCWC 1 cut(s) 381
BccI CCATC 2 cut(s) 350, 620
BclI TGATCA 1 cut(s) 27
BcoDI GTCTC 1 cut(s) 551
BfaI CTAG 2 cut(s) 353, 790
BfrI CTTAAG 1 cut(s) 235
BglII AGATCT 1 cut(s) 769
BmiI GGNNCC 1 cut(s) 184
BmsI GCATC 1 cut(s) 210
BpiI GAAGAC 1 cut(s) 427
Bpu10I CCTNAGC 1 cut(s) 241
BpuEI CTTGAG 4 cut(s) 56, 174, 422, 665
Bsa29I ATCGAT 1 cut(s) 561
BsaHI GRCGYC 1 cut(s) 492
BsaJI CCNNGG 1 cut(s) 178
BsaXI ACNNNNNCTCC 2 cut(s) 167, 197
Bsc4I CCNNNNNNNGG 3 cut(s) 143, 508, 675
Bse3DI GCAATG 1 cut(s) 468
BseCI ATCGAT 1 cut(s) 561
BseDI CCNNGG 1 cut(s) 178
BseGI GGATG 1 cut(s) 336
BseLI CCNNNNNNNGG 3 cut(s) 143, 508, 675
BseMI GCAATG 1 cut(s) 468
BseMII CTCAG 1 cut(s) 303
BseRI GAGGAG 1 cut(s) 175
BshFI GGCC 1 cut(s) 531
BshVI ATCGAT 1 cut(s) 561
BsiHKAI GWGCWC 1 cut(s) 381
BslFI GGGAC 4 cut(s) 47, 179, 263, 628
BslI CCNNNNNNNGG 3 cut(s) 143, 508, 675
BsmAI GTCTC 1 cut(s) 551
BsmFI GGGAC 4 cut(s) 47, 179, 263, 628
BsmI GAATGC 1 cut(s) 214
BsnI GGCC 1 cut(s) 531
Bsp1286I GDGCHC 1 cut(s) 381
Bsp143I GATC 4 cut(s) 27, 391, 550, 769
BspACI CCGC 1 cut(s) 597
BspANI GGCC 1 cut(s) 531
BspCNI CTCAG 1 cut(s) 302
BspDI ATCGAT 1 cut(s) 561
BspLI GGNNCC 1 cut(s) 184
BspPI GGATC 1 cut(s) 386
BspQI GCTCTTC 1 cut(s) 136
BspTI CTTAAG 1 cut(s) 235
BsrDI GCAATG 1 cut(s) 468
BssECI CCNNGG 1 cut(s) 178
BssMI GATC 4 cut(s) 27, 391, 550, 769
BssNI GRCGYC 1 cut(s) 492
Bst4CI ACNGT 2 cut(s) 254, 543
Bst6I CTCTTC 1 cut(s) 136
BstACI GRCGYC 1 cut(s) 492
BstAFI CTTAAG 1 cut(s) 235
BstC8I GCNNGC 2 cut(s) 613, 670
BstDEI CTNAG 3 cut(s) 241, 289, 607
BstENI CCTNNNNNAGG 1 cut(s) 673
BstF5I GGATG 1 cut(s) 336
BstKTI GATC 4 cut(s) 30, 394, 553, 772
BstMAI GTCTC 1 cut(s) 551
BstMBI GATC 4 cut(s) 27, 391, 550, 769
BstMWI GCNNNNNNNGC 1 cut(s) 470
BstV2I GAAGAC 1 cut(s) 427
BstX2I RGATCY 1 cut(s) 769
BstYI RGATCY 1 cut(s) 769
Bsu15I ATCGAT 1 cut(s) 561
BsuRI GGCC 1 cut(s) 531
BsuTUI ATCGAT 1 cut(s) 561
BtsCI GGATG 1 cut(s) 336
BtsI GCAGTG 1 cut(s) 663
BtsIMutI CAGTG 3 cut(s) 259, 548, 663
Cac8I GCNNGC 2 cut(s) 613, 670
ClaI ATCGAT 1 cut(s) 561
Csp6I GTAC 1 cut(s) 346
CviAII CATG 2 cut(s) 300, 701
CviQI GTAC 1 cut(s) 346
DdeI CTNAG 3 cut(s) 241, 289, 607
DpnI GATC 4 cut(s) 29, 393, 552, 771
DpnII GATC 4 cut(s) 27, 391, 550, 769
DrdI GACNNNNNNGTC 2 cut(s) 449, 491
DseDI GACNNNNNNGTC 2 cut(s) 449, 491
EaeI YGGCCR 1 cut(s) 529
Eam1104I CTCTTC 1 cut(s) 136
EarI CTCTTC 1 cut(s) 136
Eco57I CTGAAG 1 cut(s) 108
EcoNI CCTNNNNNAGG 1 cut(s) 673
EcoRI GAATTC 1 cut(s) 43
FaeI CATG 2 cut(s) 303, 704
FaiI YATR 7 cut(s) 26, 301, 428, 528, 675, 702, 753
FaqI GGGAC 4 cut(s) 47, 179, 263, 628
FatI CATG 2 cut(s) 299, 700
FauI CCCGC 1 cut(s) 590
FbaI TGATCA 1 cut(s) 27
FokI GGATG 1 cut(s) 323
FspBI CTAG 2 cut(s) 353, 790
HaeIII GGCC 1 cut(s) 531
Hin1I GRCGYC 1 cut(s) 492
Hin1II CATG 2 cut(s) 303, 704
HindIII AAGCTT 3 cut(s) 20, 238, 613
HinfI GANTC 3 cut(s) 296, 574, 728
HphI GGTGA 2 cut(s) 22, 126
Hpy166II GTNNAC 1 cut(s) 689
Hpy188I TCNGA 2 cut(s) 391, 409
Hpy188III TCNNGA 7 cut(s) 205, 353, 395, 496, 548, 571, 682
Hpy8I GTNNAC 1 cut(s) 689
HpyAV CCTTC 5 cut(s) 104, 112, 294, 595, 707
HpyCH4III ACNGT 2 cut(s) 254, 543
HpyCH4IV ACGT 1 cut(s) 492
HpyCH4V TGCA 2 cut(s) 212, 414
HpyF10VI GCNNNNNNNGC 1 cut(s) 470
HpyF3I CTNAG 3 cut(s) 241, 289, 607
HpySE526I ACGT 1 cut(s) 492
Hsp92I GRCGYC 1 cut(s) 492
Hsp92II CATG 2 cut(s) 303, 704
Ksp22I TGATCA 1 cut(s) 27
Kzo9I GATC 4 cut(s) 27, 391, 550, 769
LguI GCTCTTC 1 cut(s) 136
LmnI GCTCC 2 cut(s) 188, 478
LpnPI CCDG 5 cut(s) 111, 353, 509, 660, 682
LweI GCATC 1 cut(s) 210
MaeI CTAG 2 cut(s) 353, 790
MaeII ACGT 1 cut(s) 492
MaeIII GTNAC 2 cut(s) 272, 304
MalI GATC 4 cut(s) 29, 393, 552, 771
MboI GATC 4 cut(s) 27, 391, 550, 769
MboII GAAGA 5 cut(s) 123, 312, 432, 532, 652
MfeI CAATTG 1 cut(s) 51
MflI RGATCY 1 cut(s) 769
MhlI GDGCHC 1 cut(s) 381
MlsI TGGCCA 1 cut(s) 531
MluCI AATT 4 cut(s) 43, 51, 148, 780
MluNI TGGCCA 1 cut(s) 531
MlyI GAGTC 1 cut(s) 568
MnlI CCTC 5 cut(s) 188, 196, 239, 298, 446
Mox20I TGGCCA 1 cut(s) 531
MscI TGGCCA 1 cut(s) 531
MseI TTAA 2 cut(s) 69, 236
MslI CAYNNNNRTG 2 cut(s) 219, 541
Msp20I TGGCCA 1 cut(s) 531
MspCI CTTAAG 1 cut(s) 235
MunI CAATTG 1 cut(s) 51
Mva1269I GAATGC 1 cut(s) 214
MwoI GCNNNNNNNGC 1 cut(s) 470
NdeII GATC 4 cut(s) 27, 391, 550, 769
NlaIII CATG 2 cut(s) 303, 704
NlaIV GGNNCC 1 cut(s) 184
NmeAIII GCCGAG 1 cut(s) 159
OliI CACNNNNGTG 1 cut(s) 541
PciSI GCTCTTC 1 cut(s) 136
PctI GAATGC 1 cut(s) 214
PfeI GAWTC 2 cut(s) 296, 728
PflFI GACNNNGTC 2 cut(s) 59, 254
PflMI CCANNNNNTGG 2 cut(s) 143, 508
PleI GAGTC 1 cut(s) 568
PpsI GAGTC 1 cut(s) 568
PsiI TTATAA 1 cut(s) 428
PspN4I GGNNCC 1 cut(s) 184
PsuI RGATCY 1 cut(s) 769
PsyI GACNNNGTC 2 cut(s) 59, 254
RsaI GTAC 1 cut(s) 347
RsaNI GTAC 1 cut(s) 346
RseI CAYNNNNRTG 2 cut(s) 219, 541
SapI GCTCTTC 1 cut(s) 136
SaqAI TTAA 2 cut(s) 69, 236
Sau3AI GATC 4 cut(s) 27, 391, 550, 769
SchI GAGTC 1 cut(s) 568
SduI GDGCHC 1 cut(s) 381
SfaNI GCATC 1 cut(s) 210
SmiMI CAYNNNNRTG 2 cut(s) 219, 541
SmlI CTYRAG 6 cut(s) 17, 35, 189, 235, 437, 680
SmoI CTYRAG 6 cut(s) 17, 35, 189, 235, 437, 680
Sse9I AATT 4 cut(s) 43, 51, 148, 780
SsiI CCGC 1 cut(s) 597
SspMI CTAG 2 cut(s) 353, 790
TaaI ACNGT 2 cut(s) 254, 543
TaiI ACGT 1 cut(s) 495
TaqI TCGA 3 cut(s) 111, 549, 561
TasI AATT 4 cut(s) 43, 51, 148, 780
TatI WGTACW 1 cut(s) 345
TfiI GAWTC 2 cut(s) 296, 728
Tru1I TTAA 2 cut(s) 69, 236
Tru9I TTAA 2 cut(s) 69, 236
TscAI CASTG 3 cut(s) 259, 548, 670
TspDTI ATGAA 4 cut(s) 288, 547, 689, 768
TspGWI ACGGA 1 cut(s) 465
TspRI CASTG 3 cut(s) 259, 548, 670
Tth111I GACNNNGTC 2 cut(s) 59, 254
Van91I CCANNNNNTGG 2 cut(s) 143, 508
Vha464I CTTAAG 1 cut(s) 235
XagI CCTNNNNNAGG 1 cut(s) 673
XapI RAATTY 2 cut(s) 43, 148
XbaI TCTAGA 1 cut(s) 352
XspI CTAG 2 cut(s) 353, 790
ZraI GACGTC 1 cut(s) 493
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.