MD11G1036700.v1.1

E3 ubiquitin-protein ligase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0008375
Physical Location & Seq
Forward (+)
3175555 .. 3178606
3052 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1036700.v1.1.491

Sequence Viewer

Length: 1026 bp
ATGGATAGCCGTTACATTTTTCAATCAGAACCCACGTACCAATCTGCTTCCGCTGCATTTTCATTTTCCTCAAATTATCCGGGAGATGATGGTGCGGCGGTTAGTTTGGTCAGGACAAGGTCCTCTAGAGCTCCTGCTTCCTTCTTAATGAGAATGGCTATGAGAATATCTAGAGCAAGGTGGTTCACCTTCTTGAGAAGAGTCTTCCACTACCAAAATGGTTCGAGGTCCAATCTTGGATCAAATCCTTTCGATAACAGCACTTGGATGATGCTGGAATTCATGGCTTTGGTTGTTCAAATAGGCGTGACCACGATTACCTTGGCTGTTTCGAAGAAGGAGAGGCCGGTTTGGCCTATGAGAGTTTGGATTGTTGGGTATGATATTGGTTGTGTTCTCCATATGTTGGTGCTCTTTGGGCGTTACAGGCTACTTTATCTGACTCAAAGTGATGGTTTCAACCTCTCCGATTTGGAACAGCAGAGAAGCACTGAAGATTCCAGGACCACACATTTGATGAACAGGTGTAGAACCTCACTCGAGCTCTTCTTTGCCGTATGGTTTGTGATGGGTAATGTTTGGGTCTTCGATTCTCGCTTTAGTTCGTTTACTGTAGCCCCGAAACTCCATGTGCTCTGCATCTCTCTGCTTGCTTGGAATGCCATTAGCTACTCCTTTCCCTTCCTGCTGTTTGTGCTGCTATGTTGCTGTGTACCCCTAATTAGCAGCCTCCTTGGATACAACATGAACATGGGATCCGTCGATAAAGGGGCTTCTGATGACCAAATTTCTCAGCTTCCCAGCTGGAGATACGAAGAAGTTAACACCAAAATAGAGCTCAGAAATGATTGCGATTCAGGATCCCTAAATGAAGATCCAGATTGCTGCATTTGCCTGGCAAAATACCGAGACAAGGAAGAAGTACGGCGGTTGCCATGTTCCCATATGTTTCACCTCAATTGCGTCGATCATTGGCTCAGAATAATATCTTGCTGTCCTCTTTGTAAGCAAGAACTGCAGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
zf-RING_11 zf-RING_2

Protein Analysis

342

Amino Acids

39.29

Weight (kDa)

8.34

Isoelectric Point (pI)

47.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 294 - 336 1.9e-11 Ring finger domain
zf-RING_11 PF17123 294 - 322 6.3e-11 RING-like zinc finger
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 406
AciI CCGC 4 cut(s) 51, 95, 98, 928
AclWI GGATC 6 cut(s) 247, 750, 763, 855, 868, 869
AcsI RAATTY 2 cut(s) 278, 786
AcuI CTGAAG 1 cut(s) 513
AfaI GTAC 3 cut(s) 38, 714, 924
AfiI CCNNNNNNNGG 2 cut(s) 406, 913
AgsI TTSAA 3 cut(s) 23, 299, 460
AjnI CCWGG 2 cut(s) 500, 894
AloI GAACNNNNNNTCC 2 cut(s) 740, 772
AluBI AGCT 6 cut(s) 131, 544, 669, 796, 804, 838
AluI AGCT 6 cut(s) 131, 544, 669, 796, 804, 838
Alw21I GWGCWC 5 cut(s) 133, 414, 546, 636, 840
Alw26I GTCTC 1 cut(s) 903
AlwI GGATC 6 cut(s) 247, 750, 763, 855, 868, 869
Ama87I CYCGRG 1 cut(s) 539
AoxI GGCC 2 cut(s) 344, 353
ApeKI GCWGC 4 cut(s) 53, 697, 726, 885
ApoI RAATTY 2 cut(s) 278, 786
ArsI GACNNNNNNTTYG 2 cut(s) 496, 528
AspS9I GGNCC 3 cut(s) 120, 228, 504
AsuC2I CCSGG 1 cut(s) 81
AsuHPI GGTGA 2 cut(s) 178, 944
AsuII TTCGAA 1 cut(s) 332
AvaI CYCGRG 1 cut(s) 539
AvaII GGWCC 3 cut(s) 120, 228, 504
BaeI ACNNNNGTAYC 2 cut(s) 20, 53
BamHI GGATCC 2 cut(s) 755, 860
BanII GRGCYC 3 cut(s) 133, 546, 840
BbsI GAAGAC 2 cut(s) 196, 577
Bbv12I GWGCWC 5 cut(s) 133, 414, 546, 636, 840
BbvI GCAGC 4 cut(s) 40, 684, 738, 872
BccI CCATC 3 cut(s) 83, 446, 562
BceAI ACGGC 2 cut(s) 539, 941
BciT130I CCWGG 2 cut(s) 502, 896
BciVI GTATCC 1 cut(s) 731
BcnI CCSGG 1 cut(s) 81
BcoDI GTCTC 1 cut(s) 903
BfaI CTAG 2 cut(s) 126, 171
BfmI CTRYAG 2 cut(s) 612, 1016
BfuI GTATCC 1 cut(s) 731
BglI GCCNNNNNGGC 1 cut(s) 352
BisI GCNGC 5 cut(s) 54, 96, 698, 727, 886
BlsI GCNGC 5 cut(s) 55, 97, 699, 728, 887
Bme1390I CCNGG 3 cut(s) 81, 502, 896
Bme18I GGWCC 3 cut(s) 120, 228, 504
BmeT110I CYCGRG 1 cut(s) 539
BmgT120I GGNCC 3 cut(s) 120, 228, 504
BmiI GGNNCC 2 cut(s) 757, 862
BmrFI CCNGG 3 cut(s) 81, 502, 896
BmsI GCATC 2 cut(s) 261, 648
BpiI GAAGAC 2 cut(s) 196, 577
BpmI CTGGAG 1 cut(s) 826
Bpu14I TTCGAA 1 cut(s) 332
BpuEI CTTGAG 1 cut(s) 214
BpuMI CCSGG 1 cut(s) 81
BsaAI YACGTR 1 cut(s) 36
BsaJI CCNNGG 2 cut(s) 321, 733
Bsc4I CCNNNNNNNGG 2 cut(s) 406, 913
Bse118I RCCGGY 1 cut(s) 346
BseBI CCWGG 2 cut(s) 502, 896
BseDI CCNNGG 2 cut(s) 321, 733
BseGI GGATG 1 cut(s) 273
BseLI CCNNNNNNNGG 2 cut(s) 406, 913
BseMII CTCAG 3 cut(s) 806, 853, 991
BseXI GCAGC 4 cut(s) 40, 684, 738, 872
BseYI CCCAGC 1 cut(s) 800
BshFI GGCC 2 cut(s) 346, 355
BsiHKAI GWGCWC 5 cut(s) 133, 414, 546, 636, 840
BsiHKCI CYCGRG 1 cut(s) 539
BsiSI CCGG 2 cut(s) 80, 347
BslI CCNNNNNNNGG 2 cut(s) 406, 913
BsmAI GTCTC 1 cut(s) 903
BsmI GAATGC 1 cut(s) 664
BsnI GGCC 2 cut(s) 346, 355
BsoBI CYCGRG 1 cut(s) 539
Bsp119I TTCGAA 1 cut(s) 332
Bsp1286I GDGCHC 5 cut(s) 133, 414, 546, 636, 840
Bsp143I GATC 5 cut(s) 239, 755, 860, 874, 967
BspACI CCGC 4 cut(s) 51, 95, 98, 928
BspANI GGCC 2 cut(s) 346, 355
BspCNI CTCAG 3 cut(s) 805, 852, 990
BspLI GGNNCC 2 cut(s) 757, 862
BspMAI CTGCAG 1 cut(s) 1020
BspPI GGATC 6 cut(s) 247, 750, 763, 855, 868, 869
BspQI GCTCTTC 1 cut(s) 551
BspT104I TTCGAA 1 cut(s) 332
BsrFI RCCGGY 1 cut(s) 346
BssAI RCCGGY 1 cut(s) 346
BssECI CCNNGG 2 cut(s) 321, 733
BssMI GATC 5 cut(s) 239, 755, 860, 874, 967
BssT1I CCWWGG 2 cut(s) 321, 733
Bst2UI CCWGG 2 cut(s) 502, 896
Bst4CI ACNGT 1 cut(s) 613
Bst6I CTCTTC 2 cut(s) 193, 551
BstAPI GCANNNNNTGC 1 cut(s) 1015
BstBAI YACGTR 1 cut(s) 36
BstBI TTCGAA 1 cut(s) 332
BstC8I GCNNGC 1 cut(s) 651
BstDEI CTNAG 3 cut(s) 792, 839, 977
BstF5I GGATG 1 cut(s) 273
BstKTI GATC 5 cut(s) 242, 758, 863, 877, 970
BstMAI GTCTC 1 cut(s) 903
BstMBI GATC 5 cut(s) 239, 755, 860, 874, 967
BstMWI GCNNNNNNNGC 8 cut(s) 53, 352, 418, 427, 659, 694, 891, 1015
BstNI CCWGG 2 cut(s) 502, 896
BstSCI CCNGG 3 cut(s) 79, 500, 894
BstSFI CTRYAG 2 cut(s) 612, 1016
BstV1I GCAGC 4 cut(s) 40, 684, 738, 872
BstV2I GAAGAC 2 cut(s) 196, 577
BstX2I RGATCY 3 cut(s) 755, 860, 874
BstYI RGATCY 3 cut(s) 755, 860, 874
BsuI GTATCC 1 cut(s) 731
BsuRI GGCC 2 cut(s) 346, 355
BtsCI GGATG 1 cut(s) 273
BtsIMutI CAGTG 1 cut(s) 489
Cac8I GCNNGC 1 cut(s) 651
Cfr10I RCCGGY 1 cut(s) 346
Cfr13I GGNCC 3 cut(s) 120, 228, 504
CseI GACGC 1 cut(s) 952
Csp6I GTAC 3 cut(s) 37, 713, 923
CviAII CATG 5 cut(s) 283, 629, 745, 751, 936
CviQI GTAC 3 cut(s) 37, 713, 923
DdeI CTNAG 3 cut(s) 792, 839, 977
DpnI GATC 5 cut(s) 241, 757, 862, 876, 969
DpnII GATC 5 cut(s) 239, 755, 860, 874, 967
Eam1104I CTCTTC 2 cut(s) 193, 551
EarI CTCTTC 2 cut(s) 193, 551
Ecl136II GAGCTC 3 cut(s) 131, 544, 838
Eco130I CCWWGG 2 cut(s) 321, 733
Eco24I GRGCYC 3 cut(s) 133, 546, 840
Eco47I GGWCC 3 cut(s) 120, 228, 504
Eco53kI GAGCTC 3 cut(s) 131, 544, 838
Eco57I CTGAAG 1 cut(s) 513
Eco88I CYCGRG 1 cut(s) 539
EcoICRI GAGCTC 3 cut(s) 131, 544, 838
EcoO109I RGGNCCY 1 cut(s) 120
EcoRI GAATTC 1 cut(s) 278
EcoRII CCWGG 2 cut(s) 500, 894
EcoT14I CCWWGG 2 cut(s) 321, 733
EcoT38I GRGCYC 3 cut(s) 133, 546, 840
ErhI CCWWGG 2 cut(s) 321, 733
FaeI CATG 5 cut(s) 286, 632, 748, 754, 939
FatI CATG 5 cut(s) 282, 628, 744, 750, 935
FauNDI CATATG 2 cut(s) 402, 945
Fnu4HI GCNGC 5 cut(s) 54, 96, 698, 727, 886
FokI GGATG 1 cut(s) 280
FriOI GRGCYC 3 cut(s) 133, 546, 840
Fsp4HI GCNGC 5 cut(s) 54, 96, 698, 727, 886
FspBI CTAG 2 cut(s) 126, 171
GluI GCNGC 5 cut(s) 54, 96, 698, 727, 886
GsaI CCCAGC 1 cut(s) 804
GsuI CTGGAG 1 cut(s) 826
HaeIII GGCC 2 cut(s) 346, 355
HapII CCGG 2 cut(s) 80, 347
HgaI GACGC 1 cut(s) 952
Hin1II CATG 5 cut(s) 286, 632, 748, 754, 939
HincII GTYRAC 1 cut(s) 823
HindII GTYRAC 1 cut(s) 823
HinfI GANTC 5 cut(s) 201, 442, 497, 590, 854
HpaI GTTAAC 1 cut(s) 823
HpaII CCGG 2 cut(s) 80, 347
HphI GGTGA 2 cut(s) 178, 944
Hpy166II GTNNAC 4 cut(s) 186, 609, 713, 823
Hpy188I TCNGA 6 cut(s) 28, 441, 469, 778, 842, 980
Hpy188III TCNNGA 6 cut(s) 112, 126, 171, 193, 858, 878
Hpy8I GTNNAC 4 cut(s) 186, 609, 713, 823
Hpy99I CGWCG 2 cut(s) 764, 968
HpyAV CCTTC 4 cut(s) 151, 199, 331, 691
HpyCH4III ACNGT 1 cut(s) 613
HpyCH4IV ACGT 1 cut(s) 35
HpyCH4V TGCA 4 cut(s) 56, 639, 888, 1018
HpyF10VI GCNNNNNNNGC 8 cut(s) 53, 352, 418, 427, 659, 694, 891, 1015
HpyF3I CTNAG 3 cut(s) 792, 839, 977
HpySE526I ACGT 1 cut(s) 35
Hsp92II CATG 5 cut(s) 286, 632, 748, 754, 939
KspAI GTTAAC 1 cut(s) 823
Kzo9I GATC 5 cut(s) 239, 755, 860, 874, 967
LguI GCTCTTC 1 cut(s) 551
LmnI GCTCC 1 cut(s) 136
Lsp1109I GCAGC 4 cut(s) 40, 684, 738, 872
LweI GCATC 2 cut(s) 261, 648
MaeI CTAG 2 cut(s) 126, 171
MaeII ACGT 1 cut(s) 35
MaeIII GTNAC 3 cut(s) 11, 307, 422
MalI GATC 5 cut(s) 241, 757, 862, 876, 969
MboI GATC 5 cut(s) 239, 755, 860, 874, 967
MboII GAAGA 9 cut(s) 196, 210, 346, 506, 538, 577, 827, 884, 929
MfeI CAATTG 1 cut(s) 958
MflI RGATCY 3 cut(s) 755, 860, 874
MhlI GDGCHC 5 cut(s) 133, 414, 546, 636, 840
MluCI AATT 5 cut(s) 73, 278, 720, 786, 958
MlyI GAGTC 2 cut(s) 210, 436
MnlI CCTC 9 cut(s) 79, 133, 219, 336, 473, 544, 740, 965, 1008
MseI TTAA 2 cut(s) 146, 822
MslI CAYNNNNRTG 2 cut(s) 266, 749
MspA1I CMGCKG 2 cut(s) 53, 804
MspI CCGG 2 cut(s) 80, 347
MspR9I CCNGG 3 cut(s) 81, 502, 896
MunI CAATTG 1 cut(s) 958
Mva1269I GAATGC 1 cut(s) 664
MvaI CCWGG 2 cut(s) 502, 896
MwoI GCNNNNNNNGC 8 cut(s) 53, 352, 418, 427, 659, 694, 891, 1015
NciI CCSGG 1 cut(s) 81
NdeI CATATG 2 cut(s) 402, 945
NdeII GATC 5 cut(s) 239, 755, 860, 874, 967
NlaIII CATG 5 cut(s) 286, 632, 748, 754, 939
NlaIV GGNNCC 2 cut(s) 757, 862
NmuCI GTSAC 1 cut(s) 307
NspV TTCGAA 1 cut(s) 332
PaeR7I CTCGAG 1 cut(s) 539
PciSI GCTCTTC 1 cut(s) 551
PctI GAATGC 1 cut(s) 664
PfeI GAWTC 3 cut(s) 497, 590, 854
PflFI GACNNNGTC 1 cut(s) 118
PflMI CCANNNNNTGG 1 cut(s) 406
PfoI TCCNGGA 2 cut(s) 79, 500
PkrI GCNGC 5 cut(s) 55, 97, 699, 728, 887
PleI GAGTC 2 cut(s) 209, 436
PpsI GAGTC 2 cut(s) 209, 436
Ppu21I YACGTR 1 cut(s) 36
PpuMI RGGWCCY 1 cut(s) 120
Psp124BI GAGCTC 3 cut(s) 133, 546, 840
Psp5II RGGWCCY 1 cut(s) 120
Psp6I CCWGG 2 cut(s) 500, 894
PspFI CCCAGC 1 cut(s) 800
PspGI CCWGG 2 cut(s) 500, 894
PspN4I GGNNCC 2 cut(s) 757, 862
PspPI GGNCC 3 cut(s) 120, 228, 504
PspPPI RGGWCCY 1 cut(s) 120
PspXI VCTCGAGB 1 cut(s) 539
PstI CTGCAG 1 cut(s) 1020
PsuI RGATCY 3 cut(s) 755, 860, 874
PsyI GACNNNGTC 1 cut(s) 118
PvuII CAGCTG 1 cut(s) 804
RsaI GTAC 3 cut(s) 38, 714, 924
RsaNI GTAC 3 cut(s) 37, 713, 923
RseI CAYNNNNRTG 2 cut(s) 266, 749
SacI GAGCTC 3 cut(s) 133, 546, 840
SapI GCTCTTC 1 cut(s) 551
SaqAI TTAA 2 cut(s) 146, 822
SatI GCNGC 5 cut(s) 54, 96, 698, 727, 886
Sau3AI GATC 5 cut(s) 239, 755, 860, 874, 967
Sau96I GGNCC 3 cut(s) 120, 228, 504
SchI GAGTC 2 cut(s) 210, 436
ScrFI CCNGG 3 cut(s) 81, 502, 896
SduI GDGCHC 5 cut(s) 133, 414, 546, 636, 840
SfaNI GCATC 2 cut(s) 261, 648
SfcI CTRYAG 2 cut(s) 612, 1016
SfiI GGCCNNNNNGGCC 1 cut(s) 352
Sfr274I CTCGAG 1 cut(s) 539
SfuI TTCGAA 1 cut(s) 332
SinI GGWCC 3 cut(s) 120, 228, 504
SlaI CTCGAG 1 cut(s) 539
SmiMI CAYNNNNRTG 2 cut(s) 266, 749
SmlI CTYRAG 2 cut(s) 193, 539
SmoI CTYRAG 2 cut(s) 193, 539
Sse9I AATT 5 cut(s) 73, 278, 720, 786, 958
SsiI CCGC 4 cut(s) 51, 95, 98, 928
SspMI CTAG 2 cut(s) 126, 171
SstI GAGCTC 3 cut(s) 133, 546, 840
StyD4I CCNGG 3 cut(s) 79, 500, 894
StyI CCWWGG 2 cut(s) 321, 733
TaaI ACNGT 1 cut(s) 613
TaiI ACGT 1 cut(s) 38
TaqI TCGA 7 cut(s) 224, 252, 332, 540, 588, 762, 966
TasI AATT 5 cut(s) 73, 278, 720, 786, 958
TauI GCSGC 1 cut(s) 98
TfiI GAWTC 3 cut(s) 497, 590, 854
Tru1I TTAA 2 cut(s) 146, 822
Tru9I TTAA 2 cut(s) 146, 822
TscAI CASTG 1 cut(s) 496
TseFI GTSAC 1 cut(s) 307
TseI GCWGC 4 cut(s) 53, 697, 726, 885
Tsp45I GTSAC 1 cut(s) 307
TspDTI ATGAA 5 cut(s) 51, 271, 533, 761, 885
TspGWI ACGGA 1 cut(s) 748
TspRI CASTG 1 cut(s) 496
Tth111I GACNNNGTC 1 cut(s) 118
Van91I CCANNNNNTGG 1 cut(s) 406
VpaK11BI GGWCC 3 cut(s) 120, 228, 504
XapI RAATTY 2 cut(s) 278, 786
XbaI TCTAGA 2 cut(s) 125, 170
XcmI CCANNNNNNNNNTGG 2 cut(s) 215, 319
XhoI CTCGAG 1 cut(s) 539
XspI CTAG 2 cut(s) 126, 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.