MD03G1036900.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008954
Physical Location & Seq
Forward (+)
2939125 .. 2943091
3967 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1036900.v1.1.491

Sequence Viewer

Length: 3288 bp
ATGCCTGCAACTCTAAGGAATCTCTTGCTTTCTCCCAATATCTTCTCCATCGCCTTACTTCTTTCAATAAACTCTCTCTTCTTCTTTTCTTCTTGTTACTCCATTGATGAACAAGGCCAAGCTCTTCTATCATGGAAGAACAGCTTAAACGGCTCCACGGACACATTGAGGTCGTGGAATCCTTCCGACACAAGTCCATGCAGCTGGTTCGGGGTTCGCTGCAGCTCCAACGGAGAAGTGGAGGAGATAACGTTGAAGGCATTGGAATTGCAAGGTCCATTGCCTTCAAACTTTCAACCTCTCAAGTCCTTAAAGACTCTTGTCCTCTCTTCGACGAATCTCACTGGCCCGATTCCAAAACAGTTTGGAGAGTATCACGAGCTTAGGGTCGTTGATCTCAGTGACAATTCTCTCTCTGGTGAAATCCCAGAAGAAATATGCAGCCTAACCCAGCTGCAAACTTTATCTCTCAATACGAATCTTTTCGAAGGCAAAATCCCTTCCGGCATCGGAAATCTTTCGCGCCTTGTGTATCTTACACTCTATGACAATCAACTCAGCGGCGAAATTCCAAAGAGTGTTGGAGAATTGAGAAAGCTAGAAGTTTTCAGAGCAGGAGGGAACAAGAATCTCAATGGTGAGATACCTTGGGAGATTGGAAACTGCACCAACTTGGTCATGTTAGGCCTTGCTGAAACCGGCATTACCGGAAGCCTTCCCACGTCGATTGGAATGCTCAAAAGACTCCGAACGATAGCCTTATACACATCTCTGCTGTCTGGTCCAATCCCTGAAGAAATCGGAAACTGCAGCGAGCTGCTGAACCTGTACTTGTATCAGAACTCCATCACCGGTCCAATCCCAAAGCGAATTGGAGAGCTCGGGAAGCTTCAGAGTCTGTTGCTGTGGCAGAACAGCTTGGTGGGTTCAATCCCAAATGAGCTTGGAAGCTGCAGAGAGCTCACAGTCTTGGACTTCTCAGAAAACCTTCTGACAGGACAAATACCTAAAAGCTTTGGTGAGCTTTCGAATCTTCAAGAGCTTCAGCTGAGTGTCAATCAGTTATCAGGTACTATACCTTCTGAAATCTCAAACTGCACTGCTCTGACTCAATTGGAAGTTGACAACAACGACATCTCGGGGGAGATTCCTGCTCTCATTGGCAACCTCAAGGGCTTAACTCTATTTTTCGCGTGGCAAAATAGGCTTACGGGCAACATTCCTGAGAGTCTCTCAGACTGTCAGGATCTTCAGGCTCTTGATCTTTCTTACAACAACCTATTTGGTTCAATACCAAGAAATCTTTTCGGATTACGAAATCTCACAAAGATTCTGCTGCTTTCCAATGATTTATCTGGCTTTATACCGCCGGATATAGGAAACTGCACAAACCTGTACAGGTTAAGGTTGAATCGTAATCGGCTGGCAGGTACTGTTCCATCGGAGATTGGCAACTTGAAGAGATTGAATTTCGTTGATTTGAGCAACAACCGCCTCGTTGGAGCAATCCCTCCATCGATATCAGGATGTCAGAACCTTGAGTTTCTTGATCTCCATTCGAATGGGATCGCCGGCTCTGTCCCTGACACACTGCCTAAAAGCCTGCAGTTTGTTGACATCTCCGATAATAGGCTCACCGGTCAGCTTCCTCCCAGCATTGGATCATTAACCGAATTGACAAAACTCAATCTCGGGAAGAATCAGCTCTCTGGCAGCATCCCAGCAGAGATCCTCTCTTGCAGTAAGCTTCAATTGCTAGACATTGGCAACAATGGCTTCTCAGGTGAAATTCCAAATCAACTTGGTCAAATTCCTTCGCTCGAAATATCCCTCAACCTAAGCTGCAACCTATTTTCGGGTGAAATCCCATCCGAGTTATCTAGTCTTACCAAGCTAGGAACCCTTGACATCTCCCACAACAAGCTCTCCGGAAACTTGAACACTCTCACAAACCTTCAAAACCTTGTTTCCCTCAATGTCTCTTTCAATAACTTATCCGGCGAATTGCCTAACACCCCATTCTTTCAGAAACTCCCTCTTAGTGACCTTGCTGAGAACACAGGTCTCTACATCTCCGGCGGAGTCACAACTCCAGCTGATAGGATGGGATCAAAGCACAATAGATCAGTTGTGAAGCTGATCACATCGGTTTTCATTTGTGTCACTGCAGCTCTTCTCCTGCTTGCAGTCTACACCCTAGTCCGTGCGCGAATAGCTAGCAATATTCTCAGGAAAGATGACAGCTGGGAAATAACCTTGTACCAGAAGCTCGATTTCTCTATAGATGACATTGTCAGGAGTTTGACATCATCGAATGTGATTGGCACCGGAAGCTCTGGGGTTGTATACAGGGTGACAATTCCAAATGGAGAAACTCTAGCAGTGAAGAAGATGTGGTCATCAGAAGAGTCAGGAGCATTCAATTCCGAAATTCAGACGCTCGGATCAATCAGGCACAAGAACATCATCCGGCTCTTGGGTTGGGGTTCAAACCGAAACCTGAAAATTCTGTTCTATGATTACCTTCCCAGCGGAAGCTTGAGCTCGCTTCTACATGGTTCCAGGAAAGGAGGAGCAGATTGGGAGGCTAGATACGATATTGTTTTAGGCGTGGCTCATGCACTTGCGTACTTGCACCATGACTGTGTGCCGGCTATTCTGCATGGTGATGTGAAAGCCATGAATGTTCTGTTGGGTCCTGGCCATGAGCCGTATCTTGCTGACTTTGGATTAGCTAGGATTGTGAACAGCAATGGTGGTGATGAATTTTCGAAAACAAGTCATCGGCCTCAATTGGCCGGTTCGTATGGATACATGGCTCCAGAGCATGCATCAATGCAAAGTATCACTGAGAAGAGTGATGTCTACAGTTTTGGCGTTGTTCTTTTAGAGGTACTAACAGGAAGGCATCCATTAGACCCAACTCTTCCCAAAGGTGCACATTTAGTTCAGTGGATTCGGGACCACTTAGCGTGCAAGAGAGACCCGATTGACATTCTCGATCAAAAGCTCAGGGGAAGATCTGATCCTGCGATGCACGAAATGCAGCAGACACTAGCTGTGTCGCTTCTCTGTGTCAGCAACCGTGCTGATGAACGCCCGATGATGAAGGATGTCGTTGCAATGCTCAAGGAAATTCGACATGTAGACTGCGCAAGGCCGGAACCGGAGTTGTTAAAGGGAGGAGGGTTGCAAGCAATTCTGGCATCCCCTCCTGCTAGGAAGGCAGTTTCACAATGCTCTTCTAACTACTCATTTGCCTTCTCTGAAGATTCAAGGTCTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1096

Amino Acids

119.18

Weight (kDa)

5.74

Isoelectric Point (pI)

35.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 36 - 75 9.3e-13 Leucine rich repeat N-terminal domain
LRR_14 PF23598 101 - 204 8.8e-11 Leucine-rich repeat region
LRR_8 PF13855 103 - 161 1.1e-06 Leucine rich repeat
LRR_14 PF23598 218 - 326 3.3e-09 Leucine-rich repeat region
LRR_8 PF13855 296 - 355 1.2e-06 Leucine rich repeat
LRR_8 PF13855 319 - 379 6.2e-08 Leucine rich repeat
LRR_8 PF13855 463 - 523 4.6e-07 Leucine rich repeat
LRR_14 PF23598 535 - 666 8.9e-08 Leucine-rich repeat region
PK_Tyr_Ser-Thr PF07714 770 - 1042 2.4e-33 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 771 - 974 6.7e-36 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 3154
Acc36I ACCTGC 1 cut(s) 1418
AccB1I GGYRCC 1 cut(s) 2324
AccI GTMKAC 4 cut(s) 2190, 2346, 2865, 3147
AccII CGCG 3 cut(s) 523, 1193, 2209
AccIII TCCGGA 1 cut(s) 1928
AciI CCGC 5 cut(s) 561, 1367, 1492, 2079, 2532
AclI AACGTT 1 cut(s) 251
AclWI GGATC 7 cut(s) 1254, 1574, 1669, 1723, 2116, 2452, 3020
AcoI YGGCCR 2 cut(s) 2701, 2796
AcsI RAATTY 8 cut(s) 567, 1468, 1788, 1809, 2430, 2505, 2765, 3135
AcuI CTGAAG 5 cut(s) 813, 875, 1028, 1235, 3288
AfaI GTAC 7 cut(s) 830, 1072, 1397, 1432, 2261, 2630, 2895
AfiI CCNNNNNNNGG 5 cut(s) 1376, 1629, 1658, 1855, 2476
AflIII ACRYGT 1 cut(s) 3142
AgeI ACCGGT 2 cut(s) 851, 1637
AjiI CACGTC 1 cut(s) 723
AjnI CCWGG 2 cut(s) 2561, 2698
AjuI GAANNNNNNNTTGG 2 cut(s) 2675, 2707
Alw21I GWGCWC 4 cut(s) 882, 963, 2546, 2941
Alw26I GTCTC 4 cut(s) 1235, 1984, 2069, 2976
Alw44I GTGCAC 1 cut(s) 2937
AlwI GGATC 7 cut(s) 1254, 1574, 1669, 1723, 2116, 2452, 3020
AlwNI CAGNNNCTG 2 cut(s) 898, 1433
Ama87I CYCGRG 3 cut(s) 881, 1138, 1691
Aor13HI TCCGGA 1 cut(s) 1928
AoxI GGCC 7 cut(s) 115, 346, 685, 2701, 2786, 2796, 3158
ApaLI GTGCAC 1 cut(s) 2937
ApoI RAATTY 8 cut(s) 567, 1468, 1788, 1809, 2430, 2505, 2765, 3135
AsiGI ACCGGT 2 cut(s) 851, 1637
Asp700I GAANNNNTTC 3 cut(s) 482, 517, 987
AspLEI GCGC 3 cut(s) 525, 2209, 3155
AspS9I GGNCC 6 cut(s) 275, 347, 782, 854, 2696, 2962
AsuII TTCGAA 4 cut(s) 486, 1028, 1559, 2771
AsuNHI GCTAGC 1 cut(s) 2216
AvaI CYCGRG 3 cut(s) 881, 1138, 1691
AvaII GGWCC 5 cut(s) 275, 782, 854, 2696, 2962
BaeGI GKGCMC 1 cut(s) 2941
BalI TGGCCA 1 cut(s) 2703
BanI GGYRCC 1 cut(s) 2324
BanII GRGCYC 3 cut(s) 882, 963, 2546
BarI GAAGNNNNNNTAC 2 cut(s) 1063, 1095
BauI CACGAG 1 cut(s) 377
Bbv12I GWGCWC 4 cut(s) 882, 963, 2546, 2941
BccI CCATC 6 cut(s) 56, 854, 1447, 1522, 1876, 2098
BceAI ACGGC 2 cut(s) 166, 2695
BcgI CGANNNNNNTGC 4 cut(s) 190, 224, 715, 749
BciT130I CCWGG 2 cut(s) 2563, 2700
BciVI GTATCC 1 cut(s) 2804
BclI TGATCA 1 cut(s) 2139
BcoDI GTCTC 4 cut(s) 1235, 1984, 2069, 2976
BfmI CTRYAG 7 cut(s) 220, 808, 952, 1604, 2166, 2280, 2866
BfuAI ACCTGC 1 cut(s) 1418
BfuI GTATCC 1 cut(s) 2804
BglII AGATCT 1 cut(s) 3020
Bme1390I CCNGG 2 cut(s) 2563, 2700
Bme18I GGWCC 5 cut(s) 275, 782, 854, 2696, 2962
BmeT110I CYCGRG 3 cut(s) 881, 1138, 1691
BmgBI CACGTC 1 cut(s) 723
BmgT120I GGNCC 6 cut(s) 275, 347, 782, 854, 2696, 2962
BmiI GGNNCC 8 cut(s) 154, 1900, 2326, 2560, 2697, 2820, 2963, 3165
BmrFI CCNGG 2 cut(s) 2563, 2700
BmsI GCATC 6 cut(s) 516, 1725, 2840, 2917, 3024, 3215
BmtI GCTAGC 1 cut(s) 2220
BoxI GACNNNNGTC 2 cut(s) 192, 320
BplI GAGNNNNNCTC 4 cut(s) 1217, 1249, 1718, 1750
BpmI CTGGAG 2 cut(s) 2076, 2805
Bpu10I CCTNAGC 3 cut(s) 383, 1838, 3011
Bpu14I TTCGAA 4 cut(s) 486, 1028, 1559, 2771
BpuEI CTTGAG 5 cut(s) 287, 1154, 1559, 2560, 3113
Bsa29I ATCGAT 1 cut(s) 1517
BsaI GGTCTC 2 cut(s) 2069, 2976
BsaJI CCNNGG 2 cut(s) 156, 647
BsaWI WCCGGW 6 cut(s) 707, 851, 1637, 1928, 2327, 3166
BsaXI ACNNNNNCTCC 4 cut(s) 236, 266, 1910, 1940
Bsc4I CCNNNNNNNGG 5 cut(s) 1376, 1629, 1658, 1855, 2476
Bse118I RCCGGY 6 cut(s) 698, 851, 1571, 1637, 2650, 2798
Bse1I ACTGG 1 cut(s) 349
Bse3DI GCAATG 3 cut(s) 278, 2758, 3129
BseAI TCCGGA 1 cut(s) 1928
BseBI CCWGG 2 cut(s) 2563, 2700
BseCI ATCGAT 1 cut(s) 1517
BseDI CCNNGG 2 cut(s) 156, 647
BseGI GGATG 8 cut(s) 1532, 1716, 1868, 2109, 2466, 2908, 3118, 3206
BseLI CCNNNNNNNGG 5 cut(s) 1376, 1629, 1658, 1855, 2476
BseMI GCAATG 3 cut(s) 278, 2758, 3129
BseNI ACTGG 1 cut(s) 349
BseRI GAGGAG 3 cut(s) 257, 2586, 3198
BseSI GKGCMC 1 cut(s) 2941
BseYI CCCAGC 5 cut(s) 450, 1652, 1720, 2244, 2528
BsgI GTGCAG 3 cut(s) 649, 1081, 1369
Bsh1236I CGCG 3 cut(s) 523, 1193, 2209
BshFI GGCC 7 cut(s) 117, 348, 687, 2703, 2788, 2798, 3160
BshNI GGYRCC 1 cut(s) 2324
BshTI ACCGGT 2 cut(s) 851, 1637
BshVI ATCGAT 1 cut(s) 1517
BsiHKAI GWGCWC 4 cut(s) 882, 963, 2546, 2941
BsiHKCI CYCGRG 3 cut(s) 881, 1138, 1691
BslFI GGGAC 2 cut(s) 1565, 2975
BslI CCNNNNNNNGG 5 cut(s) 1376, 1629, 1658, 1855, 2476
BsmAI GTCTC 4 cut(s) 1235, 1984, 2069, 2976
BsmFI GGGAC 2 cut(s) 1565, 2975
BsmI GAATGC 2 cut(s) 738, 2417
BsnI GGCC 7 cut(s) 117, 348, 687, 2703, 2788, 2798, 3160
Bso31I GGTCTC 2 cut(s) 2069, 2976
BsoBI CYCGRG 3 cut(s) 881, 1138, 1691
Bsp119I TTCGAA 4 cut(s) 486, 1028, 1559, 2771
Bsp1286I GDGCHC 4 cut(s) 882, 963, 2546, 2941
Bsp13I TCCGGA 1 cut(s) 1928
Bsp1407I TGTACA 1 cut(s) 1395
BspACI CCGC 5 cut(s) 561, 1367, 1492, 2079, 2532
BspANI GGCC 7 cut(s) 117, 348, 687, 2703, 2788, 2798, 3160
BspDI ATCGAT 1 cut(s) 1517
BspEI TCCGGA 1 cut(s) 1928
BspFNI CGCG 3 cut(s) 523, 1193, 2209
BspLI GGNNCC 8 cut(s) 154, 1900, 2326, 2560, 2697, 2820, 2963, 3165
BspMAI CTGCAG 5 cut(s) 224, 812, 956, 1608, 2170
BspMI ACCTGC 1 cut(s) 1418
BspOI GCTAGC 1 cut(s) 2220
BspPI GGATC 7 cut(s) 1254, 1574, 1669, 1723, 2116, 2452, 3020
BspQI GCTCTTC 3 cut(s) 129, 2178, 3247
BspT104I TTCGAA 4 cut(s) 486, 1028, 1559, 2771
BspT107I GGYRCC 1 cut(s) 2324
BspTNI GGTCTC 2 cut(s) 2069, 2976
BsrDI GCAATG 3 cut(s) 278, 2758, 3129
BsrFI RCCGGY 6 cut(s) 698, 851, 1571, 1637, 2650, 2798
BsrGI TGTACA 1 cut(s) 1395
BsrI ACTGG 1 cut(s) 349
BssAI RCCGGY 6 cut(s) 698, 851, 1571, 1637, 2650, 2798
BssECI CCNNGG 2 cut(s) 156, 647
BssNAI GTATAC 1 cut(s) 2347
BssSI CACGAG 1 cut(s) 377
BssT1I CCWWGG 1 cut(s) 647
Bst1107I GTATAC 1 cut(s) 2347
Bst2BI CACGAG 1 cut(s) 377
Bst2UI CCWGG 2 cut(s) 2563, 2700
Bst4CI ACNGT 7 cut(s) 363, 967, 1241, 1435, 2645, 2870, 3086
Bst6I CTCTTC 9 cut(s) 83, 129, 334, 1454, 2178, 2400, 2849, 2931, 3247
BstAPI GCANNNNNTGC 1 cut(s) 3043
BstAUI TGTACA 1 cut(s) 1395
BstBI TTCGAA 4 cut(s) 486, 1028, 1559, 2771
BstDSI CCRYGG 1 cut(s) 156
BstF5I GGATG 8 cut(s) 1532, 1716, 1868, 2109, 2466, 2908, 3118, 3206
BstFNI CGCG 3 cut(s) 523, 1193, 2209
BstHHI GCGC 3 cut(s) 525, 2209, 3155
BstMAI GTCTC 4 cut(s) 1235, 1984, 2069, 2976
BstNI CCWGG 2 cut(s) 2563, 2700
BstNSI RCATGY 2 cut(s) 2831, 3146
BstPAI GACNNNNGTC 2 cut(s) 192, 320
BstSCI CCNGG 2 cut(s) 2561, 2698
BstSFI CTRYAG 7 cut(s) 220, 808, 952, 1604, 2166, 2280, 2866
BstSLI GKGCMC 1 cut(s) 2941
BstUI CGCG 3 cut(s) 523, 1193, 2209
BstX2I RGATCY 3 cut(s) 1246, 1728, 3020
BstXI CCANNNNNNTGG 2 cut(s) 204, 1562
BstYI RGATCY 3 cut(s) 1246, 1728, 3020
BstZ17I GTATAC 1 cut(s) 2347
Bsu15I ATCGAT 1 cut(s) 1517
BsuI GTATCC 1 cut(s) 2804
BsuRI GGCC 7 cut(s) 117, 348, 687, 2703, 2788, 2798, 3160
BsuTUI ATCGAT 1 cut(s) 1517
BtgI CCRYGG 1 cut(s) 156
BtgZI GCGATG 2 cut(s) 34, 3047
BtrI CACGTC 1 cut(s) 723
BtsCI GGATG 8 cut(s) 1532, 1716, 1868, 2109, 2466, 2908, 3118, 3206
BtsI GCAGTG 4 cut(s) 1098, 1589, 2163, 2388
BtsIMutI CAGTG 8 cut(s) 342, 406, 1098, 1589, 2163, 2388, 2847, 2957
BveI ACCTGC 1 cut(s) 1418
CaiI CAGNNNCTG 2 cut(s) 898, 1433
CfoI GCGC 3 cut(s) 525, 2209, 3155
Cfr10I RCCGGY 6 cut(s) 698, 851, 1571, 1637, 2650, 2798
Cfr13I GGNCC 6 cut(s) 275, 347, 782, 854, 2696, 2962
ClaI ATCGAT 1 cut(s) 1517
CseI GACGC 1 cut(s) 2446
Csp6I GTAC 7 cut(s) 829, 1071, 1396, 1431, 2260, 2629, 2894
CspAI ACCGGT 2 cut(s) 851, 1637
CspCI CAANNNNNGTGG 2 cut(s) 709, 744
CviQI GTAC 7 cut(s) 829, 1071, 1396, 1431, 2260, 2629, 2894
EaeI YGGCCR 2 cut(s) 2701, 2796
Eam1104I CTCTTC 9 cut(s) 83, 129, 334, 1454, 2178, 2400, 2849, 2931, 3247
EarI CTCTTC 9 cut(s) 83, 129, 334, 1454, 2178, 2400, 2849, 2931, 3247
EciI GGCGGA 1 cut(s) 2094
Ecl136II GAGCTC 3 cut(s) 880, 961, 2544
Eco130I CCWWGG 1 cut(s) 647
Eco147I AGGCCT 1 cut(s) 687
Eco24I GRGCYC 3 cut(s) 882, 963, 2546
Eco31I GGTCTC 2 cut(s) 2069, 2976
Eco32I GATATC 1 cut(s) 1521
Eco47I GGWCC 5 cut(s) 275, 782, 854, 2696, 2962
Eco53kI GAGCTC 3 cut(s) 880, 961, 2544
Eco57I CTGAAG 5 cut(s) 813, 875, 1028, 1235, 3288
Eco88I CYCGRG 3 cut(s) 881, 1138, 1691
EcoICRI GAGCTC 3 cut(s) 880, 961, 2544
EcoO109I RGGNCCY 1 cut(s) 2696
EcoRII CCWGG 2 cut(s) 2561, 2698
EcoRV GATATC 1 cut(s) 1521
EcoT14I CCWWGG 1 cut(s) 647
EcoT22I ATGCAT 1 cut(s) 2833
EcoT38I GRGCYC 3 cut(s) 882, 963, 2546
ErhI CCWWGG 1 cut(s) 647
FalI AAGNNNNNCTT 2 cut(s) 128, 160
FaqI GGGAC 2 cut(s) 1565, 2975
FbaI TGATCA 1 cut(s) 2139
FblI GTMKAC 4 cut(s) 2190, 2346, 2865, 3147
FokI GGATG 8 cut(s) 1539, 1703, 1855, 2116, 2453, 2895, 3125, 3193
FriOI GRGCYC 3 cut(s) 882, 963, 2546
FspI TGCGCA 1 cut(s) 3154
GlaI GCGC 3 cut(s) 524, 2208, 3154
GsaI CCCAGC 5 cut(s) 454, 1656, 1724, 2248, 2532
GsuI CTGGAG 2 cut(s) 2076, 2805
HaeIII GGCC 7 cut(s) 117, 348, 687, 2703, 2788, 2798, 3160
HgaI GACGC 1 cut(s) 2446
HhaI GCGC 3 cut(s) 525, 2209, 3155
Hin6I GCGC 3 cut(s) 523, 2207, 3153
HinP1I GCGC 3 cut(s) 523, 2207, 3153
HincII GTYRAC 2 cut(s) 1123, 1615
HindII GTYRAC 2 cut(s) 1123, 1615
HindIII AAGCTT 4 cut(s) 887, 1012, 1745, 2536
Hpy166II GTNNAC 8 cut(s) 1123, 1615, 2191, 2347, 2746, 2866, 2939, 3148
Hpy8I GTNNAC 8 cut(s) 1123, 1615, 2191, 2347, 2746, 2866, 2939, 3148
Hpy99I CGWCG 2 cut(s) 337, 727
HpyCH4III ACNGT 7 cut(s) 363, 967, 1241, 1435, 2645, 2870, 3086
HpyCH4IV ACGT 2 cut(s) 251, 722
HpySE526I ACGT 2 cut(s) 251, 722
HspAI GCGC 3 cut(s) 523, 2207, 3153
Kpn2I TCCGGA 1 cut(s) 1928
KroI GCCGGC 2 cut(s) 1571, 2650
KroNI GCCGGC 2 cut(s) 1573, 2652
Ksp22I TGATCA 1 cut(s) 2139
LguI GCTCTTC 3 cut(s) 129, 2178, 3247
LmnI GCTCC 6 cut(s) 158, 230, 1502, 2414, 2573, 2824
LweI GCATC 6 cut(s) 516, 1725, 2840, 2917, 3024, 3215
MaeII ACGT 2 cut(s) 251, 722
MaeIII GTNAC 6 cut(s) 95, 401, 2042, 2083, 2161, 2353
MfeI CAATTG 3 cut(s) 1112, 1751, 2792
MflI RGATCY 3 cut(s) 1246, 1728, 3020
MhlI GDGCHC 4 cut(s) 882, 963, 2546, 2941
MlsI TGGCCA 1 cut(s) 2703
MluNI TGGCCA 1 cut(s) 2703
MlyI GAGTC 7 cut(s) 310, 738, 904, 1102, 1237, 2091, 2417
MmeI TCCRAC 4 cut(s) 210, 252, 562, 1480
Mox20I TGGCCA 1 cut(s) 2703
Mph1103I ATGCAT 1 cut(s) 2833
MroI TCCGGA 1 cut(s) 1928
MroNI GCCGGC 2 cut(s) 1571, 2650
MroXI GAANNNNTTC 3 cut(s) 482, 517, 987
MscI TGGCCA 1 cut(s) 2703
MseI TTAA 6 cut(s) 146, 311, 1178, 1403, 1667, 3176
MslI CAYNNNNRTG 3 cut(s) 1560, 2643, 2667
Msp20I TGGCCA 1 cut(s) 2703
MspA1I CMGCKG 7 cut(s) 204, 454, 561, 1048, 2096, 2244, 2532
MspR9I CCNGG 2 cut(s) 2563, 2700
MunI CAATTG 3 cut(s) 1112, 1751, 2792
Mva1269I GAATGC 2 cut(s) 738, 2417
MvaI CCWGG 2 cut(s) 2563, 2700
MvnI CGCG 3 cut(s) 523, 1193, 2209
NaeI GCCGGC 2 cut(s) 1573, 2652
NgoMIV GCCGGC 2 cut(s) 1571, 2650
NheI GCTAGC 1 cut(s) 2216
NlaIV GGNNCC 8 cut(s) 154, 1900, 2326, 2560, 2697, 2820, 2963, 3165
NmuCI GTSAC 5 cut(s) 401, 2042, 2083, 2161, 2353
NsbI TGCGCA 1 cut(s) 3154
NsiI ATGCAT 1 cut(s) 2833
NspI RCATGY 2 cut(s) 2831, 3146
NspV TTCGAA 4 cut(s) 486, 1028, 1559, 2771
PaeI GCATGC 1 cut(s) 2831
PceI AGGCCT 1 cut(s) 687
PciI ACATGT 1 cut(s) 3142
PciSI GCTCTTC 3 cut(s) 129, 2178, 3247
PctI GAATGC 2 cut(s) 738, 2417
PdiI GCCGGC 2 cut(s) 1573, 2652
PdmI GAANNNNTTC 3 cut(s) 482, 517, 987
PflFI GACNNNGTC 1 cut(s) 2291
PfoI TCCNGGA 1 cut(s) 2561
PinAI ACCGGT 2 cut(s) 851, 1637
PleI GAGTC 7 cut(s) 310, 738, 903, 1102, 1236, 2090, 2416
PpsI GAGTC 7 cut(s) 310, 738, 903, 1102, 1236, 2090, 2416
PpuMI RGGWCCY 1 cut(s) 2696
PscI ACATGT 1 cut(s) 3142
PshAI GACNNNNGTC 2 cut(s) 192, 320
Psp124BI GAGCTC 3 cut(s) 882, 963, 2546
Psp1406I AACGTT 1 cut(s) 251
Psp5II RGGWCCY 1 cut(s) 2696
Psp6I CCWGG 2 cut(s) 2561, 2698
PspFI CCCAGC 5 cut(s) 450, 1652, 1720, 2244, 2528
PspGI CCWGG 2 cut(s) 2561, 2698
PspN4I GGNNCC 8 cut(s) 154, 1900, 2326, 2560, 2697, 2820, 2963, 3165
PspPI GGNCC 6 cut(s) 275, 347, 782, 854, 2696, 2962
PspPPI RGGWCCY 1 cut(s) 2696
PstI CTGCAG 5 cut(s) 224, 812, 956, 1608, 2170
PstNI CAGNNNCTG 2 cut(s) 898, 1433
PsuI RGATCY 3 cut(s) 1246, 1728, 3020
PsyI GACNNNGTC 1 cut(s) 2291
PvuII CAGCTG 5 cut(s) 204, 454, 1048, 2096, 2244
RsaI GTAC 7 cut(s) 830, 1072, 1397, 1432, 2261, 2630, 2895
RsaNI GTAC 7 cut(s) 829, 1071, 1396, 1431, 2260, 2629, 2894
RseI CAYNNNNRTG 3 cut(s) 1560, 2643, 2667
SacI GAGCTC 3 cut(s) 882, 963, 2546
SapI GCTCTTC 3 cut(s) 129, 2178, 3247
SaqAI TTAA 6 cut(s) 146, 311, 1178, 1403, 1667, 3176
Sau96I GGNCC 6 cut(s) 275, 347, 782, 854, 2696, 2962
SchI GAGTC 7 cut(s) 310, 738, 904, 1102, 1237, 2091, 2417
ScrFI CCNGG 2 cut(s) 2563, 2700
SduI GDGCHC 4 cut(s) 882, 963, 2546, 2941
SfaNI GCATC 6 cut(s) 516, 1725, 2840, 2917, 3024, 3215
SfcI CTRYAG 7 cut(s) 220, 808, 952, 1604, 2166, 2280, 2866
SfuI TTCGAA 4 cut(s) 486, 1028, 1559, 2771
SinI GGWCC 5 cut(s) 275, 782, 854, 2696, 2962
SmiMI CAYNNNNRTG 3 cut(s) 1560, 2643, 2667
SmlI CTYRAG 5 cut(s) 302, 1169, 1538, 2539, 3128
SmoI CTYRAG 5 cut(s) 302, 1169, 1538, 2539, 3128
SphI GCATGC 1 cut(s) 2831
SseBI AGGCCT 1 cut(s) 687
SsiI CCGC 5 cut(s) 561, 1367, 1492, 2079, 2532
SspI AATATT 1 cut(s) 2224
SstI GAGCTC 3 cut(s) 882, 963, 2546
StuI AGGCCT 1 cut(s) 687
StyD4I CCNGG 2 cut(s) 2561, 2698
StyI CCWWGG 1 cut(s) 647
TaaI ACNGT 7 cut(s) 363, 967, 1241, 1435, 2645, 2870, 3086
TaiI ACGT 2 cut(s) 254, 725
TatI WGTACW 2 cut(s) 828, 1395
TauI GCSGC 1 cut(s) 564
Tru1I TTAA 6 cut(s) 146, 311, 1178, 1403, 1667, 3176
Tru9I TTAA 6 cut(s) 146, 311, 1178, 1403, 1667, 3176
TscAI CASTG 8 cut(s) 349, 406, 1105, 1596, 2170, 2388, 2854, 2957
TseFI GTSAC 5 cut(s) 401, 2042, 2083, 2161, 2353
Tsp45I GTSAC 5 cut(s) 401, 2042, 2083, 2161, 2353
TspDTI ATGAA 6 cut(s) 123, 2143, 2696, 2778, 3108, 3122
TspGWI ACGGA 3 cut(s) 173, 246, 2192
TspRI CASTG 8 cut(s) 349, 406, 1105, 1596, 2170, 2388, 2854, 2957
Tth111I GACNNNGTC 1 cut(s) 2291
VneI GTGCAC 1 cut(s) 2937
VpaK11BI GGWCC 5 cut(s) 275, 782, 854, 2696, 2962
XapI RAATTY 8 cut(s) 567, 1468, 1788, 1809, 2430, 2505, 2765, 3135
XceI RCATGY 2 cut(s) 2831, 3146
XcmI CCANNNNNNNNNTGG 1 cut(s) 235
XmiI GTMKAC 4 cut(s) 2190, 2346, 2865, 3147
XmnI GAANNNNTTC 3 cut(s) 482, 517, 987
Zsp2I ATGCAT 1 cut(s) 2833
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.