RchiOBHm_Chr5g0075461
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0008954
Physical Location & Seq
Reverse (-)
81273689 .. 81277609
3921 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35018

Sequence Viewer

Length: 3291 bp
ATGCCTGCTACTCTAAGGAATCCATTGCTTTCTCCCAATAACATCTTCTCAATAATCTTACTTCTTTCAATAAACTCTCTTTTCTTTTCTTCATGTTATTCCTTTGATGAACAAGGCCAGGCTCTTCTAGCATGGAAGAACAGTTTAAACAGCTCCACAGATGCATTGAAATCATGGGTTTCTTCAGACGCAAGTCCATGCAAGTGGTTTGGGATCCGCTGCAGCGATTCGAATGGAGAAGTCACAGAGATAACATTGAAGGCAATAGATTTGCAAGGCTCATTGCCTTCAAACTTTCAGCCTCTCAAGTCCTTGAGAAGCCTCATCATCTCTTCAACGAATCTCACTGGGACAATCCCGAAAGAGCTTGGAGATTATCATGAGCTAAGTTTCATTGATCTCGGTGACAACTCTCTCTCTGGTGAAATCCCAGAGGAAATATGCAGGCTGAGCAAACTCGAAACCTTATCTCTCAACACGAATTTCCTTGAAGGCAAAATCCCCTCAGGCATTGGAAACCTCTCAAGCCTAGTGTACCTGACACTCTACGACAATCAACTCAGCAGCGAAATCCCAAAGAGTATTGGAGCATTGAGCAAGCTACAAGTCTTCAGAGCAGGAGGGAACAAAAACCTCAATGGTGAGATTCCGTGGGAGATTGGCAACTGCACCAACTTGGTCATGTTAGGCCTTGCTGAGACCAGCATTACCGGAAGTCTTCCTTCCTCGATTGGACTACTGAAAAGTATCCAAACAATTGCCATATACACGTCTCTCTTGTCCGGTCCAATCCCAGAAGAGATTGGAAACTGCAGTGACTTGCAGAGCCTGTACTTGTACCAGAACTCAATCACCGGTCCAATCCCAAAGCAGATAGGTGAGCTCAGCAAGCTTCAGAGTCTGCTGCTGTGGCAGAACAGCTTGGTGGGTTCAATTCCAAATGAGGTAGGAAGCTGCAGTGGGCTCACAGTCATGGACTTATCCGAAAATCTTCTGACAGGCCAGATACCGAAAACTTTTGGTGAGCTTTCGAAACTTCAGGAGCTGCAGTTGAGTGTTAATCAGCTATCAGGTACTATCCCTTCTGAAATCTCAAACTGCAAGGATCTGACACACTTGGAAGTTGACAACAACGACATTTCGGGGGAGATTCCTCCTCTTATTGGCAACCTCAAGAGCTTAACTCTATTTTTCGCCTGGCAAAATAGGCTCACAGGCAACATTCCTGAGAGTCTATCTGATTGCCAGGAGCTTCAAGCTCTTGATCTATCTTACAACAACTTGTTTGGTCCAATACCAAAGAATATCTTTGGACTGAGAAATCTCACCAAGCTTCTGCTACTTTCCAATGATTTATCCGGCTTTATACCACCGGATATAGGAAACTGCTCGAGTTTGTACAGATTAAGGTTGAATCAGAACCGGCTGGCAGGTGCTATTCCAGCTGAGATTGGCAACTTGAAGAGCTTGAATTTTGTTGACATGAGCAACAACCGCCTTGTTGGAGCTGTCCCTCCAGCAATGTCAGGATGTCAAAACCTTGAGTTTCTTGATCTGCATTCAAATGGTCTCACTGGCTCTGTCCCTGATACTCTGCCTCAAAGTCTTCAGTTTGTTGACATCTCAGACAACAGGCTCAACGGTCAGCTCCCTCACAGCATTGGTTCATTAACCGAATTAACGAAACTCAATCTCGGGAAGAATCAACTTTCTGGAAGCATACCAGCTGAGATTCTTTCTTGCACCAAGCTTCAATTGCTAGACCTTGGCAACAATGGCTTCTCAGGCGAAATCCCAAAGCAACTTGGTCAAATTCCATCACTTGAAATTTCTGTCAACTTAAGCTGTAACCTTTTTTCTGGTGAGATCCCATCCCAATTCTCTGGTCTTAGCAAGCTAGGAGTCCTTGACCTCTCCCACAATAAGCTCTCAGGAAACTTAAACACTCTCACAGACCTTCAAAACCTTGTTTCCCTCAATGTCTCTAACAATGACTTCTCCGGTGAATTGCCAAACACCCCATTCTTTCGGAAACTCCCTCTTAGTGACCTTGCTGCAAACAAAGGTCTCTACATCGCCGACGGGGTTGTAACTCCAGCTGACAGAATGAGAGCCGGCCACAGCAGATCAGTTATGAAGCTAATCATGTCCATTCTCATTAGTGCTAGTGCACTACTTCTCCTGCTTGCAGTTTACACGCTAATCCGTGCCCGAATAGCCAACAACATTCCCAGGGAAGATGACAGCTGGGAAATGACCTTGTACCAGAAGCTCGAGTTCTCTGTTGATGACATTGTCAAGAATTTAACTTCATCTAATGTAATTGGCACTGGAAGCTCTGGAGTTGTATACAGGGTGACAATTCCAAATGGGGAGACACTAGCAGTGAAGAAGATGTGGTCATCAGAAGAGTCAGGAGCATTCAGTTCTGAAATTCAGACACTTGGTTCAATTAGGCACAAGAACATCATCAGGCTCCTTGGTTGGTGTTCCAACCGAAACCTGAAGCTTCTGTTCTATGATTACCTTCCCAATGGAAGCTTGAGCTCACAATTACATGGTGCTGGTAAAGGAGGACAAGATTGGGAGTCTAGATATGATGTTGTCTTAGGTGTGGCACATGCCTTATCGTACTTGCATCATGATTGTGTGCCTGCTATTTTACATGGTGATGTGAAAGCCATGAATGTGTTGTTGGGTCCTGGATATGAGCCTTGCCTTGCTGACTTTGGCTTAGCTAGGATCGTGAGTAGCAATGGTGATGATGATGAGTTATCCAAACCAAGTCAAAGGCCTCAGTTAGCTGGTTCTTATGGCTACATGGCTCCAGAGCATGCTTCGATGCAGAGGATCGATGAAAAGAGTGATGTGTACAGTTTTGGTGTGGTTCTGTTAGAGATGTTAACAGGAAGGCATCCACTAGACCCAACTTTGCCTGGTGGTGCACATTTAGTTCAATGGGTTCGTGAGCACTTGGCCGCAAAGCGAGACCCATCAGACATTCTTGACTCAAAGCTTAGAGGAAGAGCTGATCCTACAATGCACGAAATGCAACAAACACTAGCTGTGTCATTTCTTTGTGTCAGCACTCGTTCCAGCGATCGTCCCACTATGAAAGATGTTGTGGCAATGCTCAAAGAAATTCGACACGTTGACACTGCAAGGTCAGAGCCAGAAATGTCAAAGGGAGGAGCATTGCAATCAATTCTAGCATCTCCTGCTGCTAGAAAAGCAGTTTCACAAGGCTCTTCTAACTGCTCCTTTGCTTTCTCTGAAGATTCAATGCAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1096

Amino Acids

118.73

Weight (kDa)

5.31

Isoelectric Point (pI)

37.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 36 - 75 6e-12 Leucine rich repeat N-terminal domain
LRR_14 PF23598 100 - 205 1.3e-11 Leucine-rich repeat region
LRR_14 PF23598 219 - 303 3.1e-07 Leucine-rich repeat region
LRR_8 PF13855 321 - 380 9e-09 Leucine rich repeat
LRR_8 PF13855 465 - 524 2.8e-06 Leucine rich repeat
LRR_8 PF13855 611 - 666 3e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 771 - 1044 2.5e-34 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 772 - 1042 6.7e-36 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1421
Acc36I ACCTGC 1 cut(s) 1421
AccI GTMKAC 1 cut(s) 2349
AciI CCGC 3 cut(s) 217, 1495, 2979
AclWI GGATC 7 cut(s) 208, 221, 1113, 1861, 2750, 2858, 3026
AcoI YGGCCR 2 cut(s) 2116, 2976
AcsI RAATTY 7 cut(s) 481, 1471, 1812, 1827, 2302, 2433, 3141
AcuI CTGAAG 6 cut(s) 168, 595, 878, 1022, 1592, 2525
AfaI GTAC 8 cut(s) 536, 833, 839, 1075, 1400, 2264, 2633, 2873
AfiI CCNNNNNNNGG 2 cut(s) 1163, 1379
AflII CTTAAG 1 cut(s) 1840
AflIII ACRYGT 2 cut(s) 768, 3148
AgeI ACCGGT 1 cut(s) 854
AjiI CACGTC 1 cut(s) 771
AjnI CCWGG 6 cut(s) 117, 1196, 1245, 2231, 2701, 2935
AjuI GAANNNNNNNTTGG 4 cut(s) 29, 61, 2678, 2710
Alw21I GWGCWC 5 cut(s) 885, 2173, 2549, 2947, 2973
Alw26I GTCTC 7 cut(s) 692, 777, 1574, 1987, 2072, 2369, 2982
Alw44I GTGCAC 2 cut(s) 2169, 2943
AlwI GGATC 7 cut(s) 208, 221, 1113, 1861, 2750, 2858, 3026
AlwNI CAGNNNCTG 3 cut(s) 829, 901, 1045
Ama87I CYCGRG 3 cut(s) 1390, 1694, 2273
AoxI GGCC 6 cut(s) 115, 688, 1000, 2116, 2792, 2976
ApaLI GTGCAC 2 cut(s) 2169, 2943
ApeKI GCWGC 8 cut(s) 219, 222, 564, 904, 954, 1045, 2054, 3221
ApoI RAATTY 7 cut(s) 481, 1471, 1812, 1827, 2302, 2433, 3141
AsiGI ACCGGT 1 cut(s) 854
Asp700I GAANNNNTTC 1 cut(s) 990
AspS9I GGNCC 4 cut(s) 785, 857, 1289, 2699
AsuII TTCGAA 2 cut(s) 230, 1031
AvaI CYCGRG 3 cut(s) 1390, 1694, 2273
AvaII GGWCC 4 cut(s) 785, 857, 1289, 2699
AxyI CCTNAGG 1 cut(s) 505
BaeGI GKGCMC 3 cut(s) 2173, 2212, 2947
BamHI GGATCC 1 cut(s) 213
BanII GRGCYC 3 cut(s) 885, 966, 2549
BarI GAAGNNNNNNTAC 2 cut(s) 1066, 1098
BbsI GAAGAC 3 cut(s) 601, 710, 1598
Bbv12I GWGCWC 5 cut(s) 885, 2173, 2549, 2947, 2973
BbvI GCAGC 8 cut(s) 206, 234, 576, 891, 941, 1032, 2041, 3208
BccI CCATC 3 cut(s) 1825, 1879, 3001
BciT130I CCWGG 6 cut(s) 119, 1198, 1247, 2233, 2703, 2937
BciVI GTATCC 1 cut(s) 758
BcoDI GTCTC 7 cut(s) 692, 777, 1574, 1987, 2072, 2369, 2982
BfmI CTRYAG 4 cut(s) 220, 811, 955, 1046
BfrI CTTAAG 1 cut(s) 1840
BfuAI ACCTGC 1 cut(s) 1421
BfuI GTATCC 1 cut(s) 758
BisI GCNGC 9 cut(s) 220, 223, 565, 905, 955, 1046, 2055, 2979, 3222
BlpI GCTNAGC 3 cut(s) 449, 884, 2734
BlsI GCNGC 9 cut(s) 221, 224, 566, 906, 956, 1047, 2056, 2980, 3223
Bme1390I CCNGG 6 cut(s) 119, 1198, 1247, 2233, 2703, 2937
Bme18I GGWCC 4 cut(s) 785, 857, 1289, 2699
BmeT110I CYCGRG 3 cut(s) 1390, 1694, 2273
BmgBI CACGTC 1 cut(s) 771
BmgT120I GGNCC 4 cut(s) 785, 857, 1289, 2699
BmiI GGNNCC 4 cut(s) 215, 2477, 2700, 2826
BmrFI CCNGG 6 cut(s) 119, 1198, 1247, 2233, 2703, 2937
BmrI ACTGGG 1 cut(s) 357
BmsI GCATC 5 cut(s) 151, 2647, 2832, 2923, 3221
BmuI ACTGGG 1 cut(s) 357
BoxI GACNNNNGTC 1 cut(s) 192
BpiI GAAGAC 3 cut(s) 601, 710, 1598
BplI GAGNNNNNCTC 2 cut(s) 1168, 1200
BpmI CTGGAG 4 cut(s) 1500, 2079, 2361, 2811
Bpu1102I GCTNAGC 3 cut(s) 449, 884, 2734
Bpu14I TTCGAA 2 cut(s) 230, 1031
BpuEI CTTGAG 6 cut(s) 290, 334, 508, 1157, 1562, 2563
Bsa29I ATCGAT 1 cut(s) 2853
BsaI GGTCTC 4 cut(s) 692, 1574, 2072, 2982
BsaJI CCNNGG 5 cut(s) 650, 1765, 2231, 2232, 2479
BsaWI WCCGGW 5 cut(s) 710, 782, 854, 1372, 2000
BsaXI ACNNNNNCTCC 4 cut(s) 1034, 1064, 2163, 2193
Bsc4I CCNNNNNNNGG 2 cut(s) 1163, 1379
Bse118I RCCGGY 3 cut(s) 854, 1422, 2114
Bse1I ACTGG 3 cut(s) 352, 1579, 2335
Bse21I CCTNAGG 1 cut(s) 505
Bse3DI GCAATG 6 cut(s) 23, 281, 1527, 2761, 3135, 3194
BseBI CCWGG 6 cut(s) 119, 1198, 1247, 2233, 2703, 2937
BseCI ATCGAT 1 cut(s) 2853
BseDI CCNNGG 5 cut(s) 650, 1765, 2231, 2232, 2479
BseGI GGATG 3 cut(s) 1535, 1871, 2914
BseLI CCNNNNNNNGG 2 cut(s) 1163, 1379
BseMI GCAATG 6 cut(s) 23, 281, 1527, 2761, 3135, 3194
BseNI ACTGG 3 cut(s) 352, 1579, 2335
BseRI GAGGAG 2 cut(s) 1146, 3204
BseSI GKGCMC 3 cut(s) 2173, 2212, 2947
BseXI GCAGC 8 cut(s) 206, 234, 576, 891, 941, 1032, 2041, 3208
BseYI CCCAGC 1 cut(s) 2247
BsgI GTGCAG 1 cut(s) 652
Bsh1285I CGRYCG 1 cut(s) 3103
BshFI GGCC 6 cut(s) 117, 690, 1002, 2118, 2794, 2978
BshTI ACCGGT 1 cut(s) 854
BshVI ATCGAT 1 cut(s) 2853
BsiEI CGRYCG 1 cut(s) 3103
BsiHKAI GWGCWC 5 cut(s) 885, 2173, 2549, 2947, 2973
BsiHKCI CYCGRG 3 cut(s) 1390, 1694, 2273
BsiSI CCGG 8 cut(s) 711, 783, 855, 1359, 1373, 1423, 2001, 2115
BslFI GGGAC 4 cut(s) 364, 1496, 1568, 3090
BslI CCNNNNNNNGG 2 cut(s) 1163, 1379
BsmAI GTCTC 7 cut(s) 692, 777, 1574, 1987, 2072, 2369, 2982
BsmBI CGTCTC 1 cut(s) 777
BsmFI GGGAC 4 cut(s) 364, 1496, 1568, 3090
BsmI GAATGC 2 cut(s) 1558, 2420
BsnI GGCC 6 cut(s) 117, 690, 1002, 2118, 2794, 2978
Bso31I GGTCTC 4 cut(s) 692, 1574, 2072, 2982
BsoBI CYCGRG 3 cut(s) 1390, 1694, 2273
Bsp119I TTCGAA 2 cut(s) 230, 1031
Bsp1286I GDGCHC 7 cut(s) 885, 966, 2173, 2212, 2549, 2947, 2973
Bsp1407I TGTACA 2 cut(s) 1398, 2871
Bsp1720I GCTNAGC 3 cut(s) 449, 884, 2734
BspACI CCGC 3 cut(s) 217, 1495, 2979
BspANI GGCC 6 cut(s) 117, 690, 1002, 2118, 2794, 2978
BspDI ATCGAT 1 cut(s) 2853
BspHI TCATGA 2 cut(s) 379, 2641
BspLI GGNNCC 4 cut(s) 215, 2477, 2700, 2826
BspMAI CTGCAG 4 cut(s) 224, 815, 959, 1050
BspMI ACCTGC 1 cut(s) 1421
BspPI GGATC 7 cut(s) 208, 221, 1113, 1861, 2750, 2858, 3026
BspQI GCTCTTC 4 cut(s) 129, 1457, 3019, 3253
BspT104I TTCGAA 2 cut(s) 230, 1031
BspTI CTTAAG 1 cut(s) 1840
BspTNI GGTCTC 4 cut(s) 692, 1574, 2072, 2982
BsrDI GCAATG 6 cut(s) 23, 281, 1527, 2761, 3135, 3194
BsrFI RCCGGY 3 cut(s) 854, 1422, 2114
BsrGI TGTACA 2 cut(s) 1398, 2871
BsrI ACTGG 3 cut(s) 352, 1579, 2335
BssAI RCCGGY 3 cut(s) 854, 1422, 2114
BssECI CCNNGG 5 cut(s) 650, 1765, 2231, 2232, 2479
BssNAI GTATAC 1 cut(s) 2350
BssT1I CCWWGG 2 cut(s) 1765, 2479
Bst1107I GTATAC 1 cut(s) 2350
Bst2UI CCWGG 6 cut(s) 119, 1198, 1247, 2233, 2703, 2937
Bst4CI ACNGT 4 cut(s) 143, 970, 1643, 2876
Bst6I CTCTTC 7 cut(s) 129, 337, 792, 1457, 2403, 3019, 3253
BstAFI CTTAAG 1 cut(s) 1840
BstAPI GCANNNNNTGC 2 cut(s) 3049, 3218
BstAUI TGTACA 2 cut(s) 1398, 2871
BstBI TTCGAA 2 cut(s) 230, 1031
BstDSI CCRYGG 1 cut(s) 650
BstF5I GGATG 3 cut(s) 1535, 1871, 2914
BstMAI GTCTC 7 cut(s) 692, 777, 1574, 1987, 2072, 2369, 2982
BstMCI CGRYCG 1 cut(s) 3103
BstNI CCWGG 6 cut(s) 119, 1198, 1247, 2233, 2703, 2937
BstNSI RCATGY 2 cut(s) 2624, 2837
BstPAI GACNNNNGTC 1 cut(s) 192
BstSCI CCNGG 6 cut(s) 117, 1196, 1245, 2231, 2701, 2935
BstSFI CTRYAG 4 cut(s) 220, 811, 955, 1046
BstSLI GKGCMC 3 cut(s) 2173, 2212, 2947
BstV1I GCAGC 8 cut(s) 206, 234, 576, 891, 941, 1032, 2041, 3208
BstV2I GAAGAC 3 cut(s) 601, 710, 1598
BstX2I RGATCY 3 cut(s) 213, 1105, 1866
BstXI CCANNNNNNTGG 2 cut(s) 204, 1883
BstYI RGATCY 3 cut(s) 213, 1105, 1866
BstZ17I GTATAC 1 cut(s) 2350
Bsu15I ATCGAT 1 cut(s) 2853
Bsu36I CCTNAGG 1 cut(s) 505
BsuI GTATCC 1 cut(s) 758
BsuRI GGCC 6 cut(s) 117, 690, 1002, 2118, 2794, 2978
BsuTUI ATCGAT 1 cut(s) 2853
BtgI CCRYGG 1 cut(s) 650
BtgZI GCGATG 1 cut(s) 2059
BtrI CACGTC 1 cut(s) 771
BtsCI GGATG 3 cut(s) 1535, 1871, 2914
BtsI GCAGTG 4 cut(s) 820, 964, 2391, 3156
BtsIMutI CAGTG 7 cut(s) 345, 820, 964, 1572, 2328, 2391, 3156
BveI ACCTGC 1 cut(s) 1421
CaiI CAGNNNCTG 3 cut(s) 829, 901, 1045
CciI TCATGA 2 cut(s) 379, 2641
Cfr10I RCCGGY 3 cut(s) 854, 1422, 2114
Cfr13I GGNCC 4 cut(s) 785, 857, 1289, 2699
ClaI ATCGAT 1 cut(s) 2853
CseI GACGC 1 cut(s) 197
Csp6I GTAC 8 cut(s) 535, 832, 838, 1074, 1399, 2263, 2632, 2872
CspAI ACCGGT 1 cut(s) 854
CviQI GTAC 8 cut(s) 535, 832, 838, 1074, 1399, 2263, 2632, 2872
DraI TTTAAA 1 cut(s) 147
EaeI YGGCCR 2 cut(s) 2116, 2976
Eam1104I CTCTTC 7 cut(s) 129, 337, 792, 1457, 2403, 3019, 3253
EarI CTCTTC 7 cut(s) 129, 337, 792, 1457, 2403, 3019, 3253
Ecl136II GAGCTC 2 cut(s) 883, 2547
Eco130I CCWWGG 2 cut(s) 1765, 2479
Eco147I AGGCCT 2 cut(s) 690, 2794
Eco24I GRGCYC 3 cut(s) 885, 966, 2549
Eco31I GGTCTC 4 cut(s) 692, 1574, 2072, 2982
Eco47I GGWCC 4 cut(s) 785, 857, 1289, 2699
Eco53kI GAGCTC 2 cut(s) 883, 2547
Eco57I CTGAAG 6 cut(s) 168, 595, 878, 1022, 1592, 2525
Eco81I CCTNAGG 1 cut(s) 505
Eco88I CYCGRG 3 cut(s) 1390, 1694, 2273
EcoICRI GAGCTC 2 cut(s) 883, 2547
EcoO109I RGGNCCY 1 cut(s) 2699
EcoRII CCWGG 6 cut(s) 117, 1196, 1245, 2231, 2701, 2935
EcoT14I CCWWGG 2 cut(s) 1765, 2479
EcoT22I ATGCAT 1 cut(s) 166
EcoT38I GRGCYC 3 cut(s) 885, 966, 2549
ErhI CCWWGG 2 cut(s) 1765, 2479
Esp3I CGTCTC 1 cut(s) 777
FalI AAGNNNNNCTT 4 cut(s) 706, 738, 1292, 1324
FaqI GGGAC 4 cut(s) 364, 1496, 1568, 3090
FblI GTMKAC 1 cut(s) 2349
Fnu4HI GCNGC 9 cut(s) 220, 223, 565, 905, 955, 1046, 2055, 2979, 3222
FokI GGATG 3 cut(s) 1542, 1858, 2901
FriOI GRGCYC 3 cut(s) 885, 966, 2549
Fsp4HI GCNGC 9 cut(s) 220, 223, 565, 905, 955, 1046, 2055, 2979, 3222
GluI GCNGC 9 cut(s) 220, 223, 565, 905, 955, 1046, 2055, 2979, 3222
GsaI CCCAGC 1 cut(s) 2251
GsuI CTGGAG 4 cut(s) 1500, 2079, 2361, 2811
HaeIII GGCC 6 cut(s) 117, 690, 1002, 2118, 2794, 2978
HapII CCGG 8 cut(s) 711, 783, 855, 1359, 1373, 1423, 2001, 2115
HgaI GACGC 1 cut(s) 197
HincII GTYRAC 6 cut(s) 1126, 1480, 1618, 1837, 2904, 3154
HindII GTYRAC 6 cut(s) 1126, 1480, 1618, 1837, 2904, 3154
HindIII AAGCTT 6 cut(s) 890, 1331, 1748, 2507, 2539, 3014
HpaI GTTAAC 1 cut(s) 2904
HpaII CCGG 8 cut(s) 711, 783, 855, 1359, 1373, 1423, 2001, 2115
Hpy99I CGWCG 1 cut(s) 2084
HpyAV CCTTC 8 cut(s) 253, 297, 485, 732, 1092, 1967, 2537, 2904
HpyCH4III ACNGT 4 cut(s) 143, 970, 1643, 2876
HpyCH4IV ACGT 2 cut(s) 770, 3150
HpySE526I ACGT 2 cut(s) 770, 3150
KroI GCCGGC 1 cut(s) 2114
KroNI GCCGGC 1 cut(s) 2116
KspAI GTTAAC 1 cut(s) 2904
LguI GCTCTTC 4 cut(s) 129, 1457, 3019, 3253
Lsp1109I GCAGC 8 cut(s) 206, 234, 576, 891, 941, 1032, 2041, 3208
LweI GCATC 5 cut(s) 151, 2647, 2832, 2923, 3221
MaeII ACGT 2 cut(s) 770, 3150
MaeIII GTNAC 7 cut(s) 241, 404, 815, 1847, 2045, 2089, 2356
MfeI CAATTG 2 cut(s) 756, 1754
MflI RGATCY 3 cut(s) 213, 1105, 1866
MhlI GDGCHC 7 cut(s) 885, 966, 2173, 2212, 2549, 2947, 2973
MlyI GAGTC 6 cut(s) 907, 1240, 1911, 2420, 2597, 3002
MmeI TCCRAC 2 cut(s) 1483, 2517
Mph1103I ATGCAT 1 cut(s) 166
MroNI GCCGGC 1 cut(s) 2114
MroXI GAANNNNTTC 1 cut(s) 990
MslI CAYNNNNRTG 6 cut(s) 202, 971, 1563, 2646, 2670, 2687
MspA1I CMGCKG 5 cut(s) 219, 1445, 1727, 2099, 2247
MspCI CTTAAG 1 cut(s) 1840
MspI CCGG 8 cut(s) 711, 783, 855, 1359, 1373, 1423, 2001, 2115
MspR9I CCNGG 6 cut(s) 119, 1198, 1247, 2233, 2703, 2937
MssI GTTTAAAC 1 cut(s) 147
MunI CAATTG 2 cut(s) 756, 1754
Mva1269I GAATGC 2 cut(s) 1558, 2420
MvaI CCWGG 6 cut(s) 119, 1198, 1247, 2233, 2703, 2937
NaeI GCCGGC 1 cut(s) 2116
NgoMIV GCCGGC 1 cut(s) 2114
NlaIV GGNNCC 4 cut(s) 215, 2477, 2700, 2826
NmuCI GTSAC 5 cut(s) 241, 404, 815, 2045, 2356
NsiI ATGCAT 1 cut(s) 166
NspI RCATGY 2 cut(s) 2624, 2837
NspV TTCGAA 2 cut(s) 230, 1031
PaeI GCATGC 1 cut(s) 2837
PaeR7I CTCGAG 2 cut(s) 1390, 2273
PagI TCATGA 2 cut(s) 379, 2641
PaqCI CACCTGC 1 cut(s) 1421
PasI CCCWGGG 1 cut(s) 2232
PceI AGGCCT 2 cut(s) 690, 2794
PciSI GCTCTTC 4 cut(s) 129, 1457, 3019, 3253
PctI GAATGC 2 cut(s) 1558, 2420
PdiI GCCGGC 1 cut(s) 2116
PdmI GAANNNNTTC 1 cut(s) 990
PfeI GAWTC 9 cut(s) 19, 227, 340, 646, 1150, 1414, 1702, 1732, 3278
PflFI GACNNNGTC 1 cut(s) 2294
PfoI TCCNGGA 1 cut(s) 2701
PinAI ACCGGT 1 cut(s) 854
PkrI GCNGC 9 cut(s) 221, 224, 566, 906, 956, 1047, 2056, 2980, 3223
Ple19I CGATCG 1 cut(s) 3103
PleI GAGTC 6 cut(s) 906, 1239, 1910, 2419, 2596, 3002
PmeI GTTTAAAC 1 cut(s) 147
PpsI GAGTC 6 cut(s) 906, 1239, 1910, 2419, 2596, 3002
PpuMI RGGWCCY 1 cut(s) 2699
PshAI GACNNNNGTC 1 cut(s) 192
Psp124BI GAGCTC 2 cut(s) 885, 2549
Psp5II RGGWCCY 1 cut(s) 2699
Psp6I CCWGG 6 cut(s) 117, 1196, 1245, 2231, 2701, 2935
PspFI CCCAGC 1 cut(s) 2247
PspGI CCWGG 6 cut(s) 117, 1196, 1245, 2231, 2701, 2935
PspN4I GGNNCC 4 cut(s) 215, 2477, 2700, 2826
PspPI GGNCC 4 cut(s) 785, 857, 1289, 2699
PspPPI RGGWCCY 1 cut(s) 2699
PspXI VCTCGAGB 2 cut(s) 1390, 2273
PstI CTGCAG 4 cut(s) 224, 815, 959, 1050
PstNI CAGNNNCTG 3 cut(s) 829, 901, 1045
PsuI RGATCY 3 cut(s) 213, 1105, 1866
PsyI GACNNNGTC 1 cut(s) 2294
PvuI CGATCG 1 cut(s) 3103
PvuII CAGCTG 4 cut(s) 1445, 1727, 2099, 2247
RsaI GTAC 8 cut(s) 536, 833, 839, 1075, 1400, 2264, 2633, 2873
RsaNI GTAC 8 cut(s) 535, 832, 838, 1074, 1399, 2263, 2632, 2872
RseI CAYNNNNRTG 6 cut(s) 202, 971, 1563, 2646, 2670, 2687
SacI GAGCTC 2 cut(s) 885, 2549
SapI GCTCTTC 4 cut(s) 129, 1457, 3019, 3253
SatI GCNGC 9 cut(s) 220, 223, 565, 905, 955, 1046, 2055, 2979, 3222
Sau96I GGNCC 4 cut(s) 785, 857, 1289, 2699
SchI GAGTC 6 cut(s) 907, 1240, 1911, 2420, 2597, 3002
ScrFI CCNGG 6 cut(s) 119, 1198, 1247, 2233, 2703, 2937
SduI GDGCHC 7 cut(s) 885, 966, 2173, 2212, 2549, 2947, 2973
SfaNI GCATC 5 cut(s) 151, 2647, 2832, 2923, 3221
SfcI CTRYAG 4 cut(s) 220, 811, 955, 1046
Sfr274I CTCGAG 2 cut(s) 1390, 2273
SfuI TTCGAA 2 cut(s) 230, 1031
SinI GGWCC 4 cut(s) 785, 857, 1289, 2699
SlaI CTCGAG 2 cut(s) 1390, 2273
SmiMI CAYNNNNRTG 6 cut(s) 202, 971, 1563, 2646, 2670, 2687
SmlI CTYRAG 9 cut(s) 305, 313, 523, 1172, 1390, 1541, 1840, 2273, 2542
SmoI CTYRAG 9 cut(s) 305, 313, 523, 1172, 1390, 1541, 1840, 2273, 2542
SphI GCATGC 1 cut(s) 2837
SseBI AGGCCT 2 cut(s) 690, 2794
SsiI CCGC 3 cut(s) 217, 1495, 2979
SstI GAGCTC 2 cut(s) 885, 2549
StuI AGGCCT 2 cut(s) 690, 2794
StyD4I CCNGG 6 cut(s) 117, 1196, 1245, 2231, 2701, 2935
StyI CCWWGG 2 cut(s) 1765, 2479
TaaI ACNGT 4 cut(s) 143, 970, 1643, 2876
TaiI ACGT 2 cut(s) 773, 3153
TaqI TCGA 9 cut(s) 230, 459, 728, 1031, 1391, 2274, 2840, 2853, 3145
TatI WGTACW 3 cut(s) 831, 1398, 2871
TauI GCSGC 1 cut(s) 2981
TfiI GAWTC 9 cut(s) 19, 227, 340, 646, 1150, 1414, 1702, 1732, 3278
TscAI CASTG 7 cut(s) 352, 820, 964, 1579, 2335, 2391, 3163
TseFI GTSAC 5 cut(s) 241, 404, 815, 2045, 2356
TseI GCWGC 8 cut(s) 219, 222, 564, 904, 954, 1045, 2054, 3221
Tsp45I GTSAC 5 cut(s) 241, 404, 815, 2045, 2356
TspDTI ATGAA 9 cut(s) 81, 123, 382, 1656, 2150, 2301, 2699, 2871, 3128
TspGWI ACGGA 2 cut(s) 639, 2195
TspRI CASTG 7 cut(s) 352, 820, 964, 1579, 2335, 2391, 3163
Tth111I GACNNNGTC 1 cut(s) 2294
Vha464I CTTAAG 1 cut(s) 1840
VneI GTGCAC 2 cut(s) 2169, 2943
VpaK11BI GGWCC 4 cut(s) 785, 857, 1289, 2699
XapI RAATTY 7 cut(s) 481, 1471, 1812, 1827, 2302, 2433, 3141
XbaI TCTAGA 1 cut(s) 2591
XceI RCATGY 2 cut(s) 2624, 2837
XhoI CTCGAG 2 cut(s) 1390, 2273
XmiI GTMKAC 1 cut(s) 2349
XmnI GAANNNNTTC 1 cut(s) 990
Zsp2I ATGCAT 1 cut(s) 166
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.