Rorug05G0439700
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0008954
Physical Location & Seq
Reverse (-)
61092378 .. 61114732
22355 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0439700.1

Sequence Viewer

Length: 4173 bp
ATGATGAATAAAGTCCAAGCCAAAACATTAGCCTTCACAAAAGAGCCTTTCATCGAAGAAGAAGGGCACTGCAGAATAGCACAATGGCAATTCAATGCTCTGTCCCAGTCAGAGATCAGCAAAATGGCTGCAACTCAAATTTTTCTGGGTCGATATTACGATAGCAATCAAAAACCAATCAAAGGAGGCTTGTTGGATCCCCGCTTGGGCCCTGCAAATAGGCAATGCGGTGACTGTGAGACATGTGGTGCGAAACTTTCAGATTGTCCTGGCCACTTTGGATATTTGGTGCTTGCTGTCCCTGTATTCAATGTTGGATATTTAAGTACTGTTGTGGACGTTTTGAAGTGCATTTGTAAGTCATGCTCTCGAATTATTTTGGATGACAAATCACGCAGTCGCTTTTTGAATATTGTGAGAGATCCCAAAAAGGATAAAAGCGTGACAATGAAATCAATAGTGAAGAAGTGTACTAGCATGGCAAGCAATAATAGATCAGTGAAGTGCCCGAAATGTGGATACATGAATGGTTCAGTGAAAAGGGCTCAAGGTATGCTGGCAATTGTTCATGGTGCTGTGGATGACTTTAAATCAGCGAGTCCTGTCATGAAGGATTCCAAGGCATCCATTAATCTCCAACCTCATATGCTTAACCCTGTTAAAGTTTTTTCTTTGTTCAAAAGAATGCTTGATGAGGACTGTGAACTTCTTAATCTTAAAAGTAGACCAGAGAACCTCATCATGACAACCATTGCTGTGCCACCTATAGCCATTCGTCCTTCCGTTCTTGTGGATGGATCACAGAGTAATGAAAATGACATTACAGAGAGGTTGAAGAGAATTATTCAATCAAACGCTAATGTTCAACTGGACCTATCAGAAGGTTCTACCACAAGATTTTTGTCAAATTGGGACGAACTTCAAGCCAATGTTGCGCTGTACATAAACAGTGATGTTCGCGGTATTCCTTATCACATGCAAGTAAATGTCAAGCCATTGAATGGTTTGGTACAGCGCCTCAAAGGGAAGACGGGCCGTTTTCGTGGAAACCTATGTGGGAAAAGGGTTGAATATACTGGCAGAACTGTTATTTCACCCGACCCTAATCTGAAAATTACTGAGGTCGGAATCCCTATCCAAATGGCTCAGATTTTAAGTTATCCAGAACGTGTGTCCCATCATAATATTGAAAAGTTGAAGCAGTGTGTATTTAATGGACCTCATAAGTACCCTGGTGCCCTAGTGCTCAAACGTCAAAATGGCGAGGAATGGTCCTTGAGGGCTGAGCGACACAGGAAGTCTCATGCTGATAATCTCCGATATGGTGACATAGTTGATCGCCACTTAGAAGATGGGGACATTGTTCTTTTCAACAGACAACCAAGCTTGCATCGGATGTCCATCATGTGTCATAGGGCAAGGATTATGCCTTGGAGAACATTGAGGTTTAATGAATCTGTATGCAACCCTTATAATGCTGATTTTGATGGTGACGAAATGAACATGCATGTGCCGCAAACAGAGGAGGCTCGAACTGAGGCTGTTATGTTGATGGGGGTGCAAAACAATCTATGCACTCCCAAAAATGGGGAGATTTTGGTTGCTTCCACTCAAGATTTTCTAACATCTTCTTTCCTTATAACAAGGAAGGACACATTTTATGACCGTGCATCTTTTTCACTCATGTGTTCGTACATGGGGGATGGCACGGAGACTCTGGATTTGCCAACACCTGCTGTAATTAAGCCAATAGAGCTATGGACAGGAAAGCAGCTATATAGTGTGTTAGTTCGCCCAAATTCAAATGTGAGAGTATATCTAAACCTAACTGTTAGAGAGAAATCCTACTCCAACACAAAAACTGAAGATGGAAGAGAAACAGAAGCAATGTGCCCAAACGATGGGTTTGTCTATTTCCGTAACAGTGAGCTATTATCTGGGCAACTTGGGAAGGGCACTTTAGGAAATGGCAATAAGGATGGACTTTTCTCTGTTCTTCTCAGAGACTATAAAGCTCATGCTGCTGCTTCTTGCATGAATCGTGTAGCTAAATTGAGTGCCCGGTGGATTGGTAATCACGGATTCTCAATTGGGATTAGTGATGTACAACCTAGTGACATCTTGTATGGTGAGAAAGAAAAAATCATTAAGAAAGGTTATGACAAATGTGACGACCAGATAAAGTTGTATAAAGAAGGACAGCTACGACTTGCACCTGGTTGTGATGCTGCCCAATCACTTGAATCTGGGATTACTGCAGTATTAAACGATATTAGGGACCAAACTGGAAAGCTGTGCATGCAAACGCTACATTGGAGAAACAGTCCCCTGATTATGTCCCAGTGTGGTTCCAAAGGATCTGCTATCAATATCAGCCAGATGGTTGCTTGTGTTGGTCAACAATCAGTTGGTGGTCGTCGTGCACCAAATGGTTTCATAGATCGAAGCCTTCCTCACTTTCCTAGAAAAGAAAATACCCCTGCAGCTAAAGGCTTTGTTGCTAGTTCCTTTTACAGTGGCTTGACAGCTACAGAGTTCTTTTTTCATACAATGGGAGGGCGAGAAGGCCTTGTGGATACAGCTGTAAAAACTGCTGACACAGGGTACATGTCTCGTAGATTGTCTAAAGTTCTAGAGGACCTCTCTGTCCAGTATGATAAAACAGTGCGGGCCGCAAATGGAGGTATTGTTCAGTTTTGTTATGGGGATGACGGTATGGATCCTGCGATGATGGAAGGAAAAAGTGGAGCTCCGTTAGACTTCTACCGATTGTTTTTCAAAGCTAAGGCCACATGCCCTGCTGAAGAAAGTAAGAGCCTTTCTCCTGATGAAGTGTCTGAAATTGTGAGAAGCAGGCTTTCGGAACAAGATATGACTCCTGATGGTGGTTGCTCTATGGGTTTTAAAAGTTCTATAGAGCAATTCCTGGATGAATATGTAAAAGCATTAAGAAAAACACAGGAGGCTTTTTTATTGAATCAAAGTCCTGGTTGGAAGGTGAAGTCTGCAACTGTAGAGAAGATTGTCCAAAATATATCTGGTGTCACTTCTAGACAGCTGAAGGTTTTTCTAGATACTTGTATTTCTCGTTATCATTCAAAAAAAGTTGATGCGGGAACAGCAATTGGAGTAATTGGAGCTCAAAGCATTGGCGAGCCTGGGACACAGATGACATTAAAAACTTTTCACTTTGCTGGAGTTGCAAGCATGAATGTTACACTTGGAGTCCCTCGTATCAAGGAAATAATAAATGGAGCAAAAAATATCCATACCCCTATCATCACTACAGTACTTGAGTGTGATAATAATCCGATGATTGCACGGATGGTAGCAGGTCGGATTGAAAAAACAAATCTAGGGCAGGTTGCTGAGTGTATAGAGATTGTAATGAGTCCAAGAGCAGCATATGTAGCCATCAAGCTTGATATGTGTGTGATACAAGATGCACACTTGTCTATAGATGCCAATGTTGTAAAAGAATCAATTTTGGGAACTCCAAGGATTAAACTGAAACATGAGCACATTAGGGTTCCAGATAATCAAACTGTTGAAGTTCATTCTCACGAGAAGGATAGAAGAAAACTCCATTTTCACCTCTTCTATTTGAAGAGTATCCTCCCCGGTGTTATAGTAAGGGGTATAAAGACAATTGAACGTGTTGTCATTGATGAAGAAAAAGTTAAAAGGGACAAAAAAGATATTGACGTTAAATGCGGAGAAGAAAAGAAGTACAAGTTATTTGCTGAAGGCAAGGGTCTTCTGGCAGTAATGAGCACTAAAGGAGTAGATGGTTGTAAAACCACAAGTAATGATATTATTGAAGTGCAGCAGACACTTGGAATTGAAGCTGCAAGAACTTGTATCATTGAGGAGATAAAGTATACTATGAAATCACACGGGATGAACATAGATGATCGTCATATGATGCTTTTAGCGGATGTAATGACATTCAGAGGTTTAGTTCTTGGAATCACAAGATTCGGCGTTCAAAAGATGGGCAAAAGTGTATTGATGCTGGCTTCATTTGAGCGAACAGATGATCATCTATTTAATGCTTGCGTTCAGGGGACAGTTGACAAAATTGAAGGAGTAAGTGAAAGCATAATCATGGGTATACCAATGCCAATAGGCACCGGAATGTTCAAAATTAAACAACGCCTCCCTGCAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1390

Amino Acids

154.57

Weight (kDa)

8.97

Isoelectric Point (pI)

38.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RNA_pol_Rpb1_1 PF04997 25 - 351 1.4e-74 RNA polymerase Rpb1, domain 1
RNA_pol_Rpb1_2 PF00623 353 - 520 5.7e-74 RNA polymerase Rpb1, domain 2
RNA_pol_Rpb1_3 PF04983 523 - 702 1.2e-37 RNA polymerase Rpb1, domain 3
RNA_pol_Rpb1_4 PF05000 730 - 833 4e-29 RNA polymerase Rpb1, domain 4
RNA_pol_Rpb1_5 PF04998 840 - 1335 1.2e-88 RNA polymerase Rpb1, domain 5
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1473, 1640
AarI CACCTGC 1 cut(s) 1741
AasI GACNNNNNNGTC 1 cut(s) 2645
Acc36I ACCTGC 3 cut(s) 1741, 3323, 3352
AccB1I GGYRCC 2 cut(s) 1235, 4130
AccB7I CCANNNNNTGG 2 cut(s) 1001, 1901
AccI GTMKAC 3 cut(s) 724, 3881, 4114
AccII CGCG 1 cut(s) 960
AciI CCGC 9 cut(s) 202, 228, 960, 1514, 2668, 2673, 3113, 3714, 3935
AclWI GGATC 7 cut(s) 191, 204, 416, 805, 2365, 2714, 2727
AcoI YGGCCR 1 cut(s) 271
AcsI RAATTY 2 cut(s) 138, 1798
AcuI CTGAAG 4 cut(s) 1884, 2823, 3080, 3765
AfiI CCNNNNNNNGG 8 cut(s) 182, 206, 515, 1001, 1586, 1587, 1901, 2885
AflIII ACRYGT 4 cut(s) 242, 1168, 2607, 3655
AjnI CCWGG 6 cut(s) 268, 1231, 2215, 2925, 2986, 3157
AleI CACNNNNGTG 1 cut(s) 1684
AloI GAACNNNNNNTCC 2 cut(s) 2673, 2705
Alw21I GWGCWC 6 cut(s) 1248, 2425, 2752, 3142, 3522, 3776
Alw26I GTCTC 5 cut(s) 233, 1305, 1706, 1998, 2616
Alw44I GTGCAC 1 cut(s) 2421
AlwI GGATC 7 cut(s) 191, 204, 416, 805, 2365, 2714, 2727
AoxI GGCC 6 cut(s) 208, 271, 1033, 2566, 2670, 2787
ApaI GGGCCC 1 cut(s) 212
ApaLI GTGCAC 1 cut(s) 2421
ApeKI GCWGC 9 cut(s) 128, 1771, 2021, 2024, 2228, 2483, 3401, 3826, 3848
ApoI RAATTY 2 cut(s) 138, 1798
ArsI GACNNNNNNTTYG 2 cut(s) 1705, 1737
AseI ATTAAT 1 cut(s) 630
AspLEI GCGC 2 cut(s) 937, 1017
AspS9I GGNCC 9 cut(s) 208, 209, 871, 1033, 1217, 1272, 2278, 2638, 2670
AsuC2I CCSGG 2 cut(s) 2062, 3621
AsuHPI GGTGA 7 cut(s) 242, 1086, 1337, 1502, 2141, 3010, 3584
AvaII GGWCC 5 cut(s) 871, 1217, 1272, 2278, 2638
BaeGI GKGCMC 8 cut(s) 69, 212, 509, 1240, 1895, 1958, 2062, 2425
BaeI ACNNNNGTAYC 2 cut(s) 2568, 2601
BalI TGGCCA 1 cut(s) 273
BamHI GGATCC 2 cut(s) 196, 2719
BanI GGYRCC 2 cut(s) 1235, 4130
BanII GRGCYC 4 cut(s) 212, 547, 2752, 3142
BarI GAAGNNNNNNTAC 2 cut(s) 2187, 2219
BauI CACGAG 1 cut(s) 3563
BbsI GAAGAC 2 cut(s) 1034, 3749
Bbv12I GWGCWC 6 cut(s) 1248, 2425, 2752, 3142, 3522, 3776
BbvI GCAGC 9 cut(s) 115, 1783, 2008, 2011, 2215, 2495, 3413, 3835, 3838
BceAI ACGGC 1 cut(s) 1020
BciT130I CCWGG 6 cut(s) 270, 1233, 2217, 2927, 2988, 3159
BciVI GTATCC 3 cut(s) 512, 2569, 3623
BclI TGATCA 1 cut(s) 4039
BcnI CCSGG 2 cut(s) 2062, 3621
BcoDI GTCTC 5 cut(s) 233, 1305, 1706, 1998, 2616
BfaI CTAG 9 cut(s) 474, 1241, 2112, 2463, 2502, 2633, 3051, 3071, 3356
BfmI CTRYAG 9 cut(s) 70, 765, 2256, 2481, 2529, 2913, 3012, 3285, 3456
BfoI RGCGCY 1 cut(s) 1018
BfuAI ACCTGC 3 cut(s) 1741, 3323, 3352
BfuI GTATCC 3 cut(s) 512, 2569, 3623
BlpI GCTNAGC 1 cut(s) 1284
BmcAI AGTACT 2 cut(s) 328, 3291
Bme1390I CCNGG 8 cut(s) 270, 1233, 2062, 2217, 2927, 2988, 3159, 3621
Bme18I GGWCC 5 cut(s) 871, 1217, 1272, 2278, 2638
BmgT120I GGNCC 9 cut(s) 208, 209, 871, 1033, 1217, 1272, 2278, 2638, 2670
BmiI GGNNCC 8 cut(s) 198, 210, 1237, 2279, 2350, 2721, 3531, 4132
BmrFI CCNGG 8 cut(s) 270, 1233, 2062, 2217, 2927, 2988, 3159, 3621
BmrI ACTGGG 2 cut(s) 100, 2335
BmsI GCATC 9 cut(s) 632, 1399, 1679, 2215, 3100, 3433, 3451, 3915, 4002
BmuI ACTGGG 2 cut(s) 100, 2335
BpiI GAAGAC 2 cut(s) 1034, 3749
BplI GAGNNNNNCTC 4 cut(s) 2627, 2659, 2806, 2838
BpmI CTGGAG 1 cut(s) 3216
Bpu10I CCTNAGC 1 cut(s) 2784
Bpu1102I GCTNAGC 1 cut(s) 1284
BpuEI CTTGAG 4 cut(s) 531, 1297, 1596, 3314
BpuMI CCSGG 2 cut(s) 2062, 3621
BsaBI GATNNNNATC 4 cut(s) 165, 1400, 3306, 4041
BsaJI CCNNGG 6 cut(s) 618, 1231, 1430, 3158, 3497, 3619
BsaWI WCCGGW 1 cut(s) 4133
BsaXI ACNNNNNCTCC 8 cut(s) 2308, 2338, 2673, 2703, 3863, 3893, 4143, 4173
Bsc4I CCNNNNNNNGG 8 cut(s) 182, 206, 515, 1001, 1586, 1587, 1901, 2885
Bse1I ACTGG 6 cut(s) 106, 873, 1081, 2290, 2341, 2650
Bse3DI GCAATG 4 cut(s) 230, 750, 1893, 4173
Bse8I GATNNNNATC 4 cut(s) 165, 1400, 3306, 4041
BseBI CCWGG 6 cut(s) 270, 1233, 2217, 2927, 2988, 3159
BseDI CCNNGG 6 cut(s) 618, 1231, 1430, 3158, 3497, 3619
BseJI GATNNNNATC 4 cut(s) 165, 1400, 3306, 4041
BseLI CCNNNNNNNGG 8 cut(s) 182, 206, 515, 1001, 1586, 1587, 1901, 2885
BseMI GCAATG 4 cut(s) 230, 750, 1893, 4173
BseMII CTCAG 6 cut(s) 1110, 1160, 1275, 1527, 2014, 3360
BseNI ACTGG 6 cut(s) 106, 873, 1081, 2290, 2341, 2650
BseRI GAGGAG 2 cut(s) 1538, 3884
BseSI GKGCMC 8 cut(s) 69, 212, 509, 1240, 1895, 1958, 2062, 2425
BseXI GCAGC 9 cut(s) 115, 1783, 2008, 2011, 2215, 2495, 3413, 3835, 3838
BsgI GTGCAG 1 cut(s) 3845
Bsh1236I CGCG 1 cut(s) 960
BshFI GGCC 6 cut(s) 210, 273, 1035, 2568, 2672, 2789
BshNI GGYRCC 2 cut(s) 1235, 4130
BsiHKAI GWGCWC 6 cut(s) 1248, 2425, 2752, 3142, 3522, 3776
BsiSI CCGG 3 cut(s) 2062, 3621, 4134
BslI CCNNNNNNNGG 8 cut(s) 182, 206, 515, 1001, 1586, 1587, 1901, 2885
BsmAI GTCTC 5 cut(s) 233, 1305, 1706, 1998, 2616
BsmI GAATGC 1 cut(s) 690
BsnI GGCC 6 cut(s) 210, 273, 1035, 2568, 2672, 2789
Bsp120I GGGCCC 1 cut(s) 208
Bsp1407I TGTACA 2 cut(s) 939, 2104
Bsp1720I GCTNAGC 1 cut(s) 1284
BspACI CCGC 9 cut(s) 202, 228, 960, 1514, 2668, 2673, 3113, 3714, 3935
BspANI GGCC 6 cut(s) 210, 273, 1035, 2568, 2672, 2789
BspCNI CTCAG 6 cut(s) 1111, 1159, 1276, 1528, 2013, 3361
BspFNI CGCG 1 cut(s) 960
BspHI TCATGA 2 cut(s) 606, 741
BspLI GGNNCC 8 cut(s) 198, 210, 1237, 2279, 2350, 2721, 3531, 4132
BspMAI CTGCAG 3 cut(s) 74, 2260, 2485
BspMI ACCTGC 3 cut(s) 1741, 3323, 3352
BspPI GGATC 7 cut(s) 191, 204, 416, 805, 2365, 2714, 2727
BspT107I GGYRCC 2 cut(s) 1235, 4130
BsrDI GCAATG 4 cut(s) 230, 750, 1893, 4173
BsrGI TGTACA 2 cut(s) 939, 2104
BsrI ACTGG 6 cut(s) 106, 873, 1081, 2290, 2341, 2650
BssECI CCNNGG 6 cut(s) 618, 1231, 1430, 3158, 3497, 3619
BssNAI GTATAC 2 cut(s) 3882, 4115
BssSI CACGAG 1 cut(s) 3563
BssT1I CCWWGG 3 cut(s) 618, 1430, 3497
Bst1107I GTATAC 2 cut(s) 3882, 4115
Bst2BI CACGAG 1 cut(s) 3563
Bst2UI CCWGG 6 cut(s) 270, 1233, 2217, 2927, 2988, 3159
Bst6I CTCTTC 4 cut(s) 830, 1867, 3602, 3602
BstAUI TGTACA 2 cut(s) 939, 2104
BstDEI CTNAG 8 cut(s) 1119, 1146, 1284, 1345, 1536, 2000, 2784, 3369
BstFNI CGCG 1 cut(s) 960
BstH2I RGCGCY 1 cut(s) 1018
BstHHI GCGC 2 cut(s) 937, 1017
BstMAI GTCTC 5 cut(s) 233, 1305, 1706, 1998, 2616
BstMWI GCNNNNNNNGC 9 cut(s) 483, 932, 1513, 1753, 2021, 2299, 3119, 3200, 3410
BstNI CCWGG 6 cut(s) 270, 1233, 2217, 2927, 2988, 3159
BstNSI RCATGY 7 cut(s) 246, 979, 1507, 1511, 2302, 2611, 2796
BstSCI CCNGG 8 cut(s) 268, 1231, 2060, 2215, 2925, 2986, 3157, 3619
BstSFI CTRYAG 9 cut(s) 70, 765, 2256, 2481, 2529, 2913, 3012, 3285, 3456
BstSLI GKGCMC 8 cut(s) 69, 212, 509, 1240, 1895, 1958, 2062, 2425
BstUI CGCG 1 cut(s) 960
BstV1I GCAGC 9 cut(s) 115, 1783, 2008, 2011, 2215, 2495, 3413, 3835, 3838
BstV2I GAAGAC 2 cut(s) 1034, 3749
BstX2I RGATCY 4 cut(s) 196, 421, 2357, 2719
BstYI RGATCY 4 cut(s) 196, 421, 2357, 2719
BstZ17I GTATAC 2 cut(s) 3882, 4115
BsuI GTATCC 3 cut(s) 512, 2569, 3623
BsuRI GGCC 6 cut(s) 210, 273, 1035, 2568, 2672, 2789
BtgZI GCGATG 1 cut(s) 2741
BtsI GCAGTG 2 cut(s) 67, 1208
BtsIMutI CAGTG 9 cut(s) 67, 504, 540, 955, 1208, 1930, 2348, 2521, 2670
BveI ACCTGC 3 cut(s) 1741, 3323, 3352
CciI TCATGA 2 cut(s) 606, 741
CfoI GCGC 2 cut(s) 937, 1017
Cfr13I GGNCC 9 cut(s) 208, 209, 871, 1033, 1217, 1272, 2278, 2638, 2670
CsiI ACCWGGT 1 cut(s) 2215
DdeI CTNAG 8 cut(s) 1119, 1146, 1284, 1345, 1536, 2000, 2784, 3369
DraI TTTAAA 2 cut(s) 589, 2905
DrdI GACNNNNNNGTC 1 cut(s) 2645
DseDI GACNNNNNNGTC 1 cut(s) 2645
EaeI YGGCCR 1 cut(s) 271
Eam1104I CTCTTC 4 cut(s) 830, 1867, 3602, 3602
EarI CTCTTC 4 cut(s) 830, 1867, 3602, 3602
Ecl136II GAGCTC 2 cut(s) 2750, 3140
Eco130I CCWWGG 3 cut(s) 618, 1430, 3497
Eco147I AGGCCT 1 cut(s) 2568
Eco24I GRGCYC 4 cut(s) 212, 547, 2752, 3142
Eco47I GGWCC 5 cut(s) 871, 1217, 1272, 2278, 2638
Eco53kI GAGCTC 2 cut(s) 2750, 3140
Eco57I CTGAAG 4 cut(s) 1884, 2823, 3080, 3765
EcoICRI GAGCTC 2 cut(s) 2750, 3140
EcoO109I RGGNCCY 2 cut(s) 209, 2638
EcoRII CCWGG 6 cut(s) 268, 1231, 2215, 2925, 2986, 3157
EcoT14I CCWWGG 3 cut(s) 618, 1430, 3497
EcoT22I ATGCAT 1 cut(s) 1509
EcoT38I GRGCYC 4 cut(s) 212, 547, 2752, 3142
ErhI CCWWGG 3 cut(s) 618, 1430, 3497
FauI CCCGC 3 cut(s) 209, 2661, 3106
FauNDI CATATG 3 cut(s) 645, 3406, 3921
FbaI TGATCA 1 cut(s) 4039
FblI GTMKAC 3 cut(s) 724, 3881, 4114
FriOI GRGCYC 4 cut(s) 212, 547, 2752, 3142
FspBI CTAG 9 cut(s) 474, 1241, 2112, 2463, 2502, 2633, 3051, 3071, 3356
GlaI GCGC 2 cut(s) 936, 1016
GsuI CTGGAG 1 cut(s) 3216
HaeII RGCGCY 1 cut(s) 1018
HaeIII GGCC 6 cut(s) 210, 273, 1035, 2568, 2672, 2789
HapII CCGG 3 cut(s) 2062, 3621, 4134
HhaI GCGC 2 cut(s) 937, 1017
Hin6I GCGC 2 cut(s) 935, 1015
HinP1I GCGC 2 cut(s) 935, 1015
HincII GTYRAC 2 cut(s) 2399, 4075
HindII GTYRAC 2 cut(s) 2399, 4075
HindIII AAGCTT 2 cut(s) 1384, 3419
HpaII CCGG 3 cut(s) 2062, 3621, 4134
HphI GGTGA 7 cut(s) 242, 1086, 1337, 1502, 2141, 3010, 3584
Hpy166II GTNNAC 9 cut(s) 337, 471, 704, 725, 2399, 2423, 3882, 4075, 4115
Hpy8I GTNNAC 9 cut(s) 337, 471, 704, 725, 2399, 2423, 3882, 4075, 4115
Hpy99I CGWCG 1 cut(s) 2421
HpyCH4IV ACGT 5 cut(s) 339, 1168, 1252, 3655, 3705
HpyF10VI GCNNNNNNNGC 9 cut(s) 483, 932, 1513, 1753, 2021, 2299, 3119, 3200, 3410
HpyF3I CTNAG 8 cut(s) 1119, 1146, 1284, 1345, 1536, 2000, 2784, 3369
HpySE526I ACGT 5 cut(s) 339, 1168, 1252, 3655, 3705
HspAI GCGC 2 cut(s) 935, 1015
Ksp22I TGATCA 1 cut(s) 4039
LmnI GCTCC 4 cut(s) 2747, 2755, 3137, 3254
Lsp1109I GCAGC 9 cut(s) 115, 1783, 2008, 2011, 2215, 2495, 3413, 3835, 3838
LweI GCATC 9 cut(s) 632, 1399, 1679, 2215, 3100, 3433, 3451, 3915, 4002
MabI ACCWGGT 1 cut(s) 2215
MaeI CTAG 9 cut(s) 474, 1241, 2112, 2463, 2502, 2633, 3051, 3071, 3356
MaeII ACGT 5 cut(s) 339, 1168, 1252, 3655, 3705
MaeIII GTNAC 9 cut(s) 230, 442, 1325, 1490, 1919, 2114, 2168, 3043, 3214
MfeI CAATTG 4 cut(s) 561, 2088, 3123, 3648
MflI RGATCY 4 cut(s) 196, 421, 2357, 2719
MlsI TGGCCA 1 cut(s) 273
MluNI TGGCCA 1 cut(s) 273
MlyI GAGTC 5 cut(s) 607, 1708, 2869, 3234, 3400
MmeI TCCRAC 7 cut(s) 174, 295, 661, 1105, 1875, 2972, 3317
Mox20I TGGCCA 1 cut(s) 273
Mph1103I ATGCAT 1 cut(s) 1509
MscI TGGCCA 1 cut(s) 273
MslI CAYNNNNRTG 5 cut(s) 755, 1508, 1684, 4106, 4136
Msp20I TGGCCA 1 cut(s) 273
MspA1I CMGCKG 2 cut(s) 2582, 3058
MspI CCGG 3 cut(s) 2062, 3621, 4134
MspR9I CCNGG 8 cut(s) 270, 1233, 2062, 2217, 2927, 2988, 3159, 3621
MunI CAATTG 4 cut(s) 561, 2088, 3123, 3648
Mva1269I GAATGC 1 cut(s) 690
MvaI CCWGG 6 cut(s) 270, 1233, 2217, 2927, 2988, 3159
MvnI CGCG 1 cut(s) 960
MwoI GCNNNNNNNGC 9 cut(s) 483, 932, 1513, 1753, 2021, 2299, 3119, 3200, 3410
NciI CCSGG 2 cut(s) 2062, 3621
NdeI CATATG 3 cut(s) 645, 3406, 3921
NlaIV GGNNCC 8 cut(s) 198, 210, 1237, 2279, 2350, 2721, 3531, 4132
NmuCI GTSAC 7 cut(s) 230, 442, 1325, 1490, 2114, 2168, 3043
NsiI ATGCAT 1 cut(s) 1509
NspI RCATGY 7 cut(s) 246, 979, 1507, 1511, 2302, 2611, 2796
OliI CACNNNNGTG 1 cut(s) 1684
PaeI GCATGC 1 cut(s) 2302
PagI TCATGA 2 cut(s) 606, 741
PaqCI CACCTGC 1 cut(s) 1741
PceI AGGCCT 1 cut(s) 2568
PciI ACATGT 2 cut(s) 242, 2607
PctI GAATGC 1 cut(s) 690
PflMI CCANNNNNTGG 2 cut(s) 1001, 1901
PfoI TCCNGGA 1 cut(s) 2925
PleI GAGTC 5 cut(s) 606, 1708, 2869, 3233, 3399
PpsI GAGTC 5 cut(s) 606, 1708, 2869, 3233, 3399
PpuMI RGGWCCY 1 cut(s) 2638
PscI ACATGT 2 cut(s) 242, 2607
PshBI ATTAAT 1 cut(s) 630
PsiI TTATAA 2 cut(s) 1473, 1640
Psp124BI GAGCTC 2 cut(s) 2752, 3142
Psp5II RGGWCCY 1 cut(s) 2638
Psp6I CCWGG 6 cut(s) 268, 1231, 2215, 2925, 2986, 3157
PspGI CCWGG 6 cut(s) 268, 1231, 2215, 2925, 2986, 3157
PspN4I GGNNCC 8 cut(s) 198, 210, 1237, 2279, 2350, 2721, 3531, 4132
PspOMI GGGCCC 1 cut(s) 208
PspPI GGNCC 9 cut(s) 208, 209, 871, 1033, 1217, 1272, 2278, 2638, 2670
PspPPI RGGWCCY 1 cut(s) 2638
PstI CTGCAG 3 cut(s) 74, 2260, 2485
PsuI RGATCY 4 cut(s) 196, 421, 2357, 2719
PvuII CAGCTG 2 cut(s) 2582, 3058
RseI CAYNNNNRTG 5 cut(s) 755, 1508, 1684, 4106, 4136
SacI GAGCTC 2 cut(s) 2752, 3142
Sau96I GGNCC 9 cut(s) 208, 209, 871, 1033, 1217, 1272, 2278, 2638, 2670
ScaI AGTACT 2 cut(s) 328, 3291
SchI GAGTC 5 cut(s) 607, 1708, 2869, 3234, 3400
ScrFI CCNGG 8 cut(s) 270, 1233, 2062, 2217, 2927, 2988, 3159, 3621
SexAI ACCWGGT 1 cut(s) 2215
SfaNI GCATC 9 cut(s) 632, 1399, 1679, 2215, 3100, 3433, 3451, 3915, 4002
SfcI CTRYAG 9 cut(s) 70, 765, 2256, 2481, 2529, 2913, 3012, 3285, 3456
SinI GGWCC 5 cut(s) 871, 1217, 1272, 2278, 2638
SmiMI CAYNNNNRTG 5 cut(s) 755, 1508, 1684, 4106, 4136
SmlI CTYRAG 4 cut(s) 546, 1276, 1611, 3293
SmoI CTYRAG 4 cut(s) 546, 1276, 1611, 3293
SphI GCATGC 1 cut(s) 2302
SseBI AGGCCT 1 cut(s) 2568
SsiI CCGC 9 cut(s) 202, 228, 960, 1514, 2668, 2673, 3113, 3714, 3935
SspI AATATT 2 cut(s) 412, 1186
SspMI CTAG 9 cut(s) 474, 1241, 2112, 2463, 2502, 2633, 3051, 3071, 3356
SstI GAGCTC 2 cut(s) 2752, 3142
StuI AGGCCT 1 cut(s) 2568
StyD4I CCNGG 8 cut(s) 268, 1231, 2060, 2215, 2925, 2986, 3157, 3619
StyI CCWWGG 3 cut(s) 618, 1430, 3497
TaiI ACGT 5 cut(s) 342, 1171, 1255, 3658, 3708
TaqI TCGA 5 cut(s) 54, 151, 370, 1531, 2443
TatI WGTACW 6 cut(s) 326, 470, 939, 2104, 3289, 3729
TauI GCSGC 2 cut(s) 1516, 2675
TscAI CASTG 9 cut(s) 74, 504, 540, 955, 1208, 1930, 2348, 2521, 2670
TseFI GTSAC 7 cut(s) 230, 442, 1325, 1490, 2114, 2168, 3043
TseI GCWGC 9 cut(s) 128, 1771, 2021, 2024, 2228, 2483, 3401, 3826, 3848
Tsp45I GTSAC 7 cut(s) 230, 442, 1325, 1490, 2114, 2168, 3043
TspGWI ACGGA 6 cut(s) 772, 1724, 1907, 2094, 2742, 3337
TspRI CASTG 9 cut(s) 74, 504, 540, 955, 1208, 1930, 2348, 2521, 2670
Van91I CCANNNNNTGG 2 cut(s) 1001, 1901
VneI GTGCAC 1 cut(s) 2421
VpaK11BI GGWCC 5 cut(s) 871, 1217, 1272, 2278, 2638
VspI ATTAAT 1 cut(s) 630
XapI RAATTY 2 cut(s) 138, 1798
XbaI TCTAGA 3 cut(s) 2632, 3050, 3070
XceI RCATGY 7 cut(s) 246, 979, 1507, 1511, 2302, 2611, 2796
XcmI CCANNNNNNNNNTGG 3 cut(s) 1349, 1755, 3035
XmiI GTMKAC 3 cut(s) 724, 3881, 4114
XspI CTAG 9 cut(s) 474, 1241, 2112, 2463, 2502, 2633, 3051, 3071, 3356
ZrmI AGTACT 2 cut(s) 328, 3291
Zsp2I ATGCAT 1 cut(s) 1509
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.