MD11G1038100.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0008954
Physical Location & Seq
Forward (+)
3261385 .. 3265072
3688 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1038100.v1.1.491

Sequence Viewer

Length: 3375 bp
ATGGGGTTCCTCCCTCTACAATCCCAAAACAAATCACCTGCTGATCTCTTTCTCTCTCTCTCTCTCTTCTCTCTTCTCTCACCAGCAATGCCTGCAACTCTAAGGAATCTCTTGCTTTCTCCCAATATCTTCTCCATCACCTTACTTCTCTCAATAAACTCTCTCTTCTTCTTTTCTTCTTGTTACTCCATTGACGAACAGGGCCAAGCTCTTCTCTCCTGGAAGAACAGCTTAAACGGCTCCACGGACGCATTGAGATCATGGAATCCTTCCGACACAAGTCCATGCAGCTGGTTCGGGGTTCGCTGCAGCTCCAATGGAGAAGTGGAGGAGATAACCTTGAAGGCATTGAATTTTCAAGGTCCATTGCCTTCAAACTTTCAGCGTCTCAAGTCCTTAAAGACCCTTGTCCTCTCTTCGACGAATCTCACTGGCACGATTCCAAAAGAATTTGGAGAGTATCGCGAGCTTAGTGTCATTGACCTCAGTGGCAATTCTCTCTCTGGTGAAATCCCGGAAGAAATATGCAGCCTAAACAAGCTGCAAACTATATCTCTCAATACAAATTTTTTCGAAGGCAAAATCCCTTCCGGCATTGGAAATCTTTCGAACCTTGTGTATCTTACACTCTACGACAATCAACTTAGCGGCGAAATTCCAAAGAGTATTGGAGAATTGAGAAAGCTAGAAGTTTTCAGAGCAGGTGGGAACAAGAATCTCAATGGTGAGATACCTTGGGAAATTGGAAACTGCACCAACTTGGTCATGTTAGGCCTTGCTGAAACCAGCATTACTGGAAGCCTTCCCTCATCAATTGGAATGCTCAAAAGACTCCAAACCGTAGCCTTGTACACATCTCTGCTATCCGGTCCAATCCCTGAAGAAATTGGAAACTGCAGCGAGCTGCAGAACCTGTACTTGTACCAGAACTCCATCACCGGTCCAATCCCAAAGCGAATTGGAGAGCTCGGAAAACTTCAGAGTCTGTTGCTGTGGCAGAACAGCTTGGTTGGTTCAATCCCAAGTGAGCTTGGAAGCTGCAGGGAGGTCACAGTCATGGACTTCTCCGAAAATCTTCTGGCAGGCCAGATACCAAAAAGTTTTGGTGAGCTTTCAAATCTTCAAGAGCTTCAGTTGAGTGTCAATCAGTTATCAGGTACTATACCTTCTGAAATATCAAACTGCACTGCTCTGACTCATTTGGAAGTTGACAACAACGACATCTCGGGGGAGATTCCTGCTCTCATTGGCAACCTCAAGGGCTTAACTCTATTTTTCGCATGGCAAAATAAGCTTACAGGCAACATTCCTGAGAGTCTCTCATACTGTCAGAATCTTCAGGGTCTTGATCTTTCTTACAACAACCTATTTGGTTCAATACCAAGAAATGTTTTCGGATTGCAAAATCTCACCAAGCTGCTGCTGCTTTCCAATGATTTATCTGGCTTTATACCGCCGGATATAGGAAACTGCACGAACCTGTACAGGTTAAGGTTGAATCATAACCGGCTTTCAGGTACTGTTCCATTGGAGATTGGCAACTTGAAGAGCTTAAATTTTGTTGATTTGAGCAACAACCGCCTCGTTGGAGAAATCCCTCCATCGATATCAGGATGTCAGAACCTCGAGTATCTTGATCTCCATTCAAATGGGATCACCGGCTCTGTCCCTGGCACTCTGCCTAAAAGCCTGCAGTTTGTTGACATCTCTGACAATAGGCTCACCGGCCAGCTTCCTCACAGCATTGGATCATTAACCGAATTGACAAAACTCAATCTCGGGAAGAATCAGCTCTCTGGAAGCATCCCTGCTGAGATCCTTTCTTGCAGTAAGCTTCAACTGCTAGACATTGGCAACAATGGCTTCTCAGGTGAAATTCCAAAGCAACTTGGTCAAATTCCTTCCCTTGAAATATCCCTCAACCTAAGCTGCAACCTATTTTCGGGTGAAATCCCATCCGAGTTCTCTGGTCTTACCAAGATAGGAATCCTTGATATTTCCCACAACAAGCTCTCCGGAAACTTAAACACTCTCACAAGCCTTCAAAACCTTGTTTCCCTCAATGTCTCTTTCAATGACTTATCCGGCGAATTGCCTAACACCCCATTCTTTCGGAAACTCCCTCTTAGTGACCTTGCTGCAAACAACGGTCTCTACATCTCCGGCGGAGTCACAACTCCAGCAGATAGGATGGGATCAAGGCACAACAGATCAGTTATGAAGCTGATCACATCGGTTCTCATTAGTGTCAGTGCAGTACTTCTCCTGCTTGCAGTCTACACACTAGTCCGCGCGCGTATAGCCGGCAACATTCTCAGGGAAGATGACAGCTGGGAAATGACCTGGTACCAGAAGCTTGATTTCTCAATAGATGACATCGTCAGGAATTTGACATCGTCGAATGTGATTGGCACCGGAAGCTCTGGAGTTGTATACAGAGTGACAATTCCGAATGGAGAAACACTAGCAGTGAAGAAGATGTGGTCATCAGAAGAGTTAGGAGCATTCAATTCCGAAATTCAAACGCTTGGATTAATTAGGCACAAGAACATCATCCGCCTCTTGGGTTGGGGTTCAAATCGAAACCTGAAAATTCTGTTCTACGATTACCTCCCCAATGGAAGCTTGAGCTCACTACTACATGGTTCAGGAAAGGGAGGAGCAGATTGGGAAGCTAGATACGATGTTGTTTTAGGCGTAGCTCATGCACTTGCGTACTTGCACCATGACTGCGTGCCGGCTATTCTGCATGGTGATGTGAAAGCCATGAACGTTCTGTTGGGTCCTGGCTATGAGCCATTTCTTGCTGACTTTGGGTTAGCTAGGATTGTGAACAGCAACGGTGATGGCGAATTTTCGAAAACAAGTCAACGGCCTCAATTAGCTGGTTCATATGGATACATGGCTCCAGAGCATGCATCGATGCAGAGTATCACTGAGAAGAGCGATGTGTACAGTTTCGGTGTTGTTCTTTTAGAGGTACTAACAGGAAGGCATCCATTAGACCCAACTCTGCCCGGAGGTGCACATTTAGTTCAATGGATTCGAGATCACTTGGCGAGCAAGCGAGACCCTATTGACATTCTCGATCAAAAGCTCAGGGGAAGATCTGATCCTACAATGCACGAAATGCTGCAGACACTAGCCGTGTCATTTCTCTGTGTCAGCACTCGCGCTGAGGAACGTCCGATGATGAAAGATGTCGTTGCAATGCTCAAGGAAATTCGACATGTAGACAGCGCAAGGCCAGAACCAGAGTTGTTAAAGGGAGGAGGGTTGCAATCAATTCTGGCATCCCCTCCTGCCAGGAGAGCAGTTTCACAAGGCTCTTCTAACTGCTCATTTGCTTTCTCTGAAGATTCAAGGTCTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

1125

Amino Acids

122.19

Weight (kDa)

5.64

Isoelectric Point (pI)

37.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 65 - 104 3.7e-13 Leucine rich repeat N-terminal domain
LRR_14 PF23598 126 - 233 3.6e-11 Leucine-rich repeat region
LRR_14 PF23598 247 - 353 2.2e-08 Leucine-rich repeat region
LRR_8 PF13855 350 - 408 2.1e-06 Leucine rich repeat
LRR_8 PF13855 492 - 552 1.6e-06 Leucine rich repeat
LRR_8 PF13855 563 - 621 6.4e-06 Leucine rich repeat
PK_Tyr_Ser-Thr PF07714 799 - 1071 1.9e-32 Protein tyrosine and serine/threonine kinase
Pkinase PF00069 800 - 1002 2.5e-34 Protein kinase domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 46, 692
Acc36I ACCTGC 2 cut(s) 46, 692
Acc65I GGTACC 1 cut(s) 2346
AccB1I GGYRCC 2 cut(s) 2346, 2411
AccI GTMKAC 3 cut(s) 2277, 2433, 3234
AccII CGCG 5 cut(s) 465, 2292, 2294, 2296, 3174
AccIII TCCGGA 1 cut(s) 2015
AciI CCGC 6 cut(s) 648, 1454, 1579, 2166, 2290, 2557
AclI AACGTT 1 cut(s) 2772
AclWI GGATC 5 cut(s) 1661, 1756, 1810, 2203, 3107
AcoI YGGCCR 1 cut(s) 1726
AcuI CTGAAG 5 cut(s) 900, 962, 1115, 1322, 3375
AfiI CCNNNNNNNGG 3 cut(s) 1463, 1942, 2563
AflIII ACRYGT 1 cut(s) 3229
AgeI ACCGGT 1 cut(s) 938
AhlI ACTAGT 1 cut(s) 2284
AjnI CCWGG 5 cut(s) 218, 1669, 2342, 2785, 3305
AjuI GAANNNNNNNTTGG 2 cut(s) 2762, 2794
Alw21I GWGCWC 3 cut(s) 969, 2633, 3028
Alw26I GTCTC 5 cut(s) 392, 1322, 2071, 2156, 3063
Alw44I GTGCAC 1 cut(s) 3024
AlwI GGATC 5 cut(s) 1661, 1756, 1810, 2203, 3107
AlwNI CAGNNNCTG 3 cut(s) 913, 985, 1520
Ama87I CYCGRG 3 cut(s) 1225, 1625, 1778
Aor13HI TCCGGA 1 cut(s) 2015
AoxI GGCC 6 cut(s) 202, 772, 1084, 1726, 2873, 3245
ApaLI GTGCAC 1 cut(s) 3024
AseI ATTAAT 1 cut(s) 2534
AsiGI ACCGGT 1 cut(s) 938
Asp700I GAANNNNTTC 2 cut(s) 604, 1074
Asp718I GGTACC 1 cut(s) 2346
AspLEI GCGC 4 cut(s) 2294, 2296, 3176, 3242
AspS9I GGNCC 5 cut(s) 202, 362, 869, 941, 2783
AsuC2I CCSGG 2 cut(s) 515, 3018
AsuII TTCGAA 3 cut(s) 573, 608, 2858
AvaI CYCGRG 3 cut(s) 1225, 1625, 1778
AvaII GGWCC 4 cut(s) 362, 869, 941, 2783
BaeGI GKGCMC 1 cut(s) 3028
BanI GGYRCC 2 cut(s) 2346, 2411
BanII GRGCYC 2 cut(s) 969, 2633
BarI GAAGNNNNNNTAC 2 cut(s) 1150, 1182
Bbv12I GWGCWC 3 cut(s) 969, 2633, 3028
BbvCI CCTCAGC 1 cut(s) 3177
BccI CCATC 6 cut(s) 143, 941, 1609, 1963, 2185, 2840
BceAI ACGGC 3 cut(s) 253, 2888, 3131
BcgI CGANNNNNNTGC 2 cut(s) 277, 311
BciT130I CCWGG 5 cut(s) 220, 1671, 2344, 2787, 3307
BciVI GTATCC 1 cut(s) 2891
BclI TGATCA 1 cut(s) 2226
BcnI CCSGG 2 cut(s) 515, 3018
BcoDI GTCTC 5 cut(s) 392, 1322, 2071, 2156, 3063
BcuI ACTAGT 1 cut(s) 2284
BfaI CTAG 7 cut(s) 686, 1844, 2285, 2465, 2676, 2823, 3143
BfmI CTRYAG 6 cut(s) 307, 895, 905, 1039, 1691, 3134
BfuAI ACCTGC 2 cut(s) 46, 692
BfuI GTATCC 1 cut(s) 2891
BglII AGATCT 1 cut(s) 3107
BmcAI AGTACT 1 cut(s) 2259
Bme1390I CCNGG 7 cut(s) 220, 515, 1671, 2344, 2787, 3018, 3307
Bme18I GGWCC 4 cut(s) 362, 869, 941, 2783
BmeT110I CYCGRG 3 cut(s) 1225, 1625, 1778
BmgT120I GGNCC 5 cut(s) 202, 362, 869, 941, 2783
BmiI GGNNCC 6 cut(s) 8, 241, 2348, 2413, 2784, 2907
BmrFI CCNGG 7 cut(s) 220, 515, 1671, 2344, 2787, 3018, 3307
BmsI GCATC 5 cut(s) 1812, 2913, 2927, 3004, 3302
BoxI GACNNNNGTC 2 cut(s) 279, 407
BplI GAGNNNNNCTC 2 cut(s) 1304, 1336
BpmI CTGGAG 3 cut(s) 2163, 2445, 2892
Bpu10I CCTNAGC 3 cut(s) 1925, 3098, 3177
Bpu14I TTCGAA 3 cut(s) 573, 608, 2858
BpuEI CTTGAG 4 cut(s) 374, 1241, 2647, 3200
BpuMI CCSGG 2 cut(s) 515, 3018
Bsa29I ATCGAT 2 cut(s) 1604, 2921
BsaBI GATNNNNATC 1 cut(s) 1985
BsaI GGTCTC 2 cut(s) 2156, 3063
BsaJI CCNNGG 3 cut(s) 243, 734, 1669
BsaWI WCCGGW 4 cut(s) 866, 938, 2015, 2414
BsaXI ACNNNNNCTCC 4 cut(s) 914, 944, 1997, 2027
Bsc4I CCNNNNNNNGG 3 cut(s) 1463, 1942, 2563
Bse118I RCCGGY 6 cut(s) 938, 1506, 1658, 1724, 2303, 2737
Bse1I ACTGG 2 cut(s) 436, 799
Bse3DI GCAATG 3 cut(s) 93, 365, 3216
Bse8I GATNNNNATC 1 cut(s) 1985
BseAI TCCGGA 1 cut(s) 2015
BseBI CCWGG 5 cut(s) 220, 1671, 2344, 2787, 3307
BseCI ATCGAT 2 cut(s) 1604, 2921
BseDI CCNNGG 3 cut(s) 243, 734, 1669
BseGI GGATG 7 cut(s) 1619, 1803, 1955, 2196, 2553, 2995, 3293
BseJI GATNNNNATC 1 cut(s) 1985
BseLI CCNNNNNNNGG 3 cut(s) 1463, 1942, 2563
BseMI GCAATG 3 cut(s) 93, 365, 3216
BseMII CTCAG 8 cut(s) 499, 1302, 1803, 1881, 2329, 2928, 3112, 3168
BseNI ACTGG 2 cut(s) 436, 799
BsePI GCGCGC 1 cut(s) 2292
BseRI GAGGAG 3 cut(s) 344, 2673, 3285
BseSI GKGCMC 1 cut(s) 3028
BseYI CCCAGC 1 cut(s) 2331
BsgI GTGCAG 4 cut(s) 736, 1168, 1456, 2274
Bsh1236I CGCG 5 cut(s) 465, 2292, 2294, 2296, 3174
BshFI GGCC 6 cut(s) 204, 774, 1086, 1728, 2875, 3247
BshNI GGYRCC 2 cut(s) 2346, 2411
BshTI ACCGGT 1 cut(s) 938
BshVI ATCGAT 2 cut(s) 1604, 2921
BsiHKAI GWGCWC 3 cut(s) 969, 2633, 3028
BsiHKCI CYCGRG 3 cut(s) 1225, 1625, 1778
BslFI GGGAC 1 cut(s) 1652
BslI CCNNNNNNNGG 3 cut(s) 1463, 1942, 2563
BsmAI GTCTC 5 cut(s) 392, 1322, 2071, 2156, 3063
BsmBI CGTCTC 1 cut(s) 392
BsmFI GGGAC 1 cut(s) 1652
BsmI GAATGC 2 cut(s) 825, 2504
BsnI GGCC 6 cut(s) 204, 774, 1086, 1728, 2875, 3247
Bso31I GGTCTC 2 cut(s) 2156, 3063
BsoBI CYCGRG 3 cut(s) 1225, 1625, 1778
Bsp119I TTCGAA 3 cut(s) 573, 608, 2858
Bsp1286I GDGCHC 3 cut(s) 969, 2633, 3028
Bsp13I TCCGGA 1 cut(s) 2015
Bsp1407I TGTACA 3 cut(s) 849, 1482, 2952
Bsp68I TCGCGA 1 cut(s) 465
BspACI CCGC 6 cut(s) 648, 1454, 1579, 2166, 2290, 2557
BspANI GGCC 6 cut(s) 204, 774, 1086, 1728, 2875, 3247
BspCNI CTCAG 8 cut(s) 498, 1303, 1804, 1880, 2328, 2929, 3111, 3169
BspDI ATCGAT 2 cut(s) 1604, 2921
BspEI TCCGGA 1 cut(s) 2015
BspFNI CGCG 5 cut(s) 465, 2292, 2294, 2296, 3174
BspLI GGNNCC 6 cut(s) 8, 241, 2348, 2413, 2784, 2907
BspMAI CTGCAG 6 cut(s) 311, 899, 909, 1043, 1695, 3138
BspMI ACCTGC 2 cut(s) 46, 692
BspPI GGATC 5 cut(s) 1661, 1756, 1810, 2203, 3107
BspQI GCTCTTC 4 cut(s) 216, 1541, 2936, 3334
BspT104I TTCGAA 3 cut(s) 573, 608, 2858
BspT107I GGYRCC 2 cut(s) 2346, 2411
BspTNI GGTCTC 2 cut(s) 2156, 3063
BsrDI GCAATG 3 cut(s) 93, 365, 3216
BsrFI RCCGGY 6 cut(s) 938, 1506, 1658, 1724, 2303, 2737
BsrGI TGTACA 3 cut(s) 849, 1482, 2952
BsrI ACTGG 2 cut(s) 436, 799
BssAI RCCGGY 6 cut(s) 938, 1506, 1658, 1724, 2303, 2737
BssECI CCNNGG 3 cut(s) 243, 734, 1669
BssHII GCGCGC 1 cut(s) 2292
BssNAI GTATAC 1 cut(s) 2434
BssT1I CCWWGG 1 cut(s) 734
Bst1107I GTATAC 1 cut(s) 2434
Bst2UI CCWGG 5 cut(s) 220, 1671, 2344, 2787, 3307
Bst4CI ACNGT 7 cut(s) 841, 1054, 1328, 1522, 2150, 2843, 2957
Bst6I CTCTTC 9 cut(s) 71, 78, 170, 216, 421, 1541, 2487, 2936, 3334
BstAPI GCANNNNNTGC 2 cut(s) 92, 3130
BstAUI TGTACA 3 cut(s) 849, 1482, 2952
BstBI TTCGAA 3 cut(s) 573, 608, 2858
BstDSI CCRYGG 1 cut(s) 243
BstF5I GGATG 7 cut(s) 1619, 1803, 1955, 2196, 2553, 2995, 3293
BstFNI CGCG 5 cut(s) 465, 2292, 2294, 2296, 3174
BstHHI GCGC 4 cut(s) 2294, 2296, 3176, 3242
BstMAI GTCTC 5 cut(s) 392, 1322, 2071, 2156, 3063
BstNI CCWGG 5 cut(s) 220, 1671, 2344, 2787, 3307
BstNSI RCATGY 2 cut(s) 2918, 3233
BstPAI GACNNNNGTC 2 cut(s) 279, 407
BstSCI CCNGG 7 cut(s) 218, 513, 1669, 2342, 2785, 3016, 3305
BstSFI CTRYAG 6 cut(s) 307, 895, 905, 1039, 1691, 3134
BstSLI GKGCMC 1 cut(s) 3028
BstUI CGCG 5 cut(s) 465, 2292, 2294, 2296, 3174
BstX2I RGATCY 2 cut(s) 1815, 3107
BstXI CCANNNNNNTGG 2 cut(s) 291, 1649
BstYI RGATCY 2 cut(s) 1815, 3107
BstZ17I GTATAC 1 cut(s) 2434
Bsu15I ATCGAT 2 cut(s) 1604, 2921
BsuI GTATCC 1 cut(s) 2891
BsuRI GGCC 6 cut(s) 204, 774, 1086, 1728, 2875, 3247
BsuTUI ATCGAT 2 cut(s) 1604, 2921
BtgI CCRYGG 1 cut(s) 243
BtgZI GCGATG 1 cut(s) 2961
BtsCI GGATG 7 cut(s) 1619, 1803, 1955, 2196, 2553, 2995, 3293
BtsI GCAGTG 2 cut(s) 1185, 2475
BtsIMutI CAGTG 6 cut(s) 429, 493, 1185, 2257, 2475, 2934
BtuMI TCGCGA 1 cut(s) 465
BveI ACCTGC 2 cut(s) 46, 692
CaiI CAGNNNCTG 3 cut(s) 913, 985, 1520
CfoI GCGC 4 cut(s) 2294, 2296, 3176, 3242
Cfr10I RCCGGY 6 cut(s) 938, 1506, 1658, 1724, 2303, 2737
Cfr13I GGNCC 5 cut(s) 202, 362, 869, 941, 2783
ClaI ATCGAT 2 cut(s) 1604, 2921
CseI GACGC 2 cut(s) 257, 374
CsiI ACCWGGT 1 cut(s) 2342
CspAI ACCGGT 1 cut(s) 938
EaeI YGGCCR 1 cut(s) 1726
Eam1104I CTCTTC 9 cut(s) 71, 78, 170, 216, 421, 1541, 2487, 2936, 3334
EarI CTCTTC 9 cut(s) 71, 78, 170, 216, 421, 1541, 2487, 2936, 3334
EciI GGCGGA 2 cut(s) 2181, 2546
Ecl136II GAGCTC 2 cut(s) 967, 2631
Eco130I CCWWGG 1 cut(s) 734
Eco147I AGGCCT 1 cut(s) 774
Eco24I GRGCYC 2 cut(s) 969, 2633
Eco31I GGTCTC 2 cut(s) 2156, 3063
Eco32I GATATC 1 cut(s) 1608
Eco47I GGWCC 4 cut(s) 362, 869, 941, 2783
Eco53kI GAGCTC 2 cut(s) 967, 2631
Eco57I CTGAAG 5 cut(s) 900, 962, 1115, 1322, 3375
Eco88I CYCGRG 3 cut(s) 1225, 1625, 1778
EcoICRI GAGCTC 2 cut(s) 967, 2631
EcoO109I RGGNCCY 1 cut(s) 2783
EcoRII CCWGG 5 cut(s) 218, 1669, 2342, 2785, 3305
EcoRV GATATC 1 cut(s) 1608
EcoT14I CCWWGG 1 cut(s) 734
EcoT22I ATGCAT 1 cut(s) 2920
EcoT38I GRGCYC 2 cut(s) 969, 2633
ErhI CCWWGG 1 cut(s) 734
Esp3I CGTCTC 1 cut(s) 392
FalI AAGNNNNNCTT 2 cut(s) 215, 247
FaqI GGGAC 1 cut(s) 1652
FauNDI CATATG 1 cut(s) 2893
FbaI TGATCA 1 cut(s) 2226
FblI GTMKAC 3 cut(s) 2277, 2433, 3234
FokI GGATG 7 cut(s) 1626, 1790, 1942, 2203, 2540, 2982, 3280
FriOI GRGCYC 2 cut(s) 969, 2633
FspBI CTAG 7 cut(s) 686, 1844, 2285, 2465, 2676, 2823, 3143
GlaI GCGC 4 cut(s) 2293, 2295, 3175, 3241
GsaI CCCAGC 1 cut(s) 2335
GsuI CTGGAG 3 cut(s) 2163, 2445, 2892
HaeIII GGCC 6 cut(s) 204, 774, 1086, 1728, 2875, 3247
HgaI GACGC 2 cut(s) 257, 374
HhaI GCGC 4 cut(s) 2294, 2296, 3176, 3242
Hin6I GCGC 4 cut(s) 2292, 2294, 3174, 3240
HinP1I GCGC 4 cut(s) 2292, 2294, 3174, 3240
HincII GTYRAC 3 cut(s) 1210, 1702, 2870
HindII GTYRAC 3 cut(s) 1210, 1702, 2870
HindIII AAGCTT 4 cut(s) 1292, 1832, 2354, 2623
Hpy99I CGWCG 2 cut(s) 424, 2401
HpyCH4III ACNGT 7 cut(s) 841, 1054, 1328, 1522, 2150, 2843, 2957
HpyCH4IV ACGT 2 cut(s) 2772, 3184
HpySE526I ACGT 2 cut(s) 2772, 3184
HspAI GCGC 4 cut(s) 2292, 2294, 3174, 3240
Kpn2I TCCGGA 1 cut(s) 2015
KpnI GGTACC 1 cut(s) 2350
KroI GCCGGC 2 cut(s) 2303, 2737
KroNI GCCGGC 2 cut(s) 2305, 2739
Ksp22I TGATCA 1 cut(s) 2226
LguI GCTCTTC 4 cut(s) 216, 1541, 2936, 3334
LmnI GCTCC 5 cut(s) 245, 317, 2501, 2660, 2911
LweI GCATC 5 cut(s) 1812, 2913, 2927, 3004, 3302
MabI ACCWGGT 1 cut(s) 2342
MaeI CTAG 7 cut(s) 686, 1844, 2285, 2465, 2676, 2823, 3143
MaeII ACGT 2 cut(s) 2772, 3184
MaeIII GTNAC 5 cut(s) 182, 1048, 2129, 2170, 2440
MauBI CGCGCGCG 1 cut(s) 2292
MfeI CAATTG 1 cut(s) 813
MflI RGATCY 2 cut(s) 1815, 3107
MhlI GDGCHC 3 cut(s) 969, 2633, 3028
MlyI GAGTC 5 cut(s) 825, 991, 1189, 1324, 2178
MmeI TCCRAC 2 cut(s) 297, 1567
Mph1103I ATGCAT 1 cut(s) 2920
MroI TCCGGA 1 cut(s) 2015
MroNI GCCGGC 2 cut(s) 2303, 2737
MroXI GAANNNNTTC 2 cut(s) 604, 1074
MseI TTAA 9 cut(s) 233, 398, 1265, 1490, 1553, 1754, 2024, 2534, 3263
MslI CAYNNNNRTG 3 cut(s) 1055, 1647, 2754
MspA1I CMGCKG 2 cut(s) 291, 2331
MspR9I CCNGG 7 cut(s) 220, 515, 1671, 2344, 2787, 3018, 3307
MunI CAATTG 1 cut(s) 813
Mva1269I GAATGC 2 cut(s) 825, 2504
MvaI CCWGG 5 cut(s) 220, 1671, 2344, 2787, 3307
MvnI CGCG 5 cut(s) 465, 2292, 2294, 2296, 3174
NaeI GCCGGC 2 cut(s) 2305, 2739
NciI CCSGG 2 cut(s) 515, 3018
NdeI CATATG 1 cut(s) 2893
NgoMIV GCCGGC 2 cut(s) 2303, 2737
NlaIV GGNNCC 6 cut(s) 8, 241, 2348, 2413, 2784, 2907
NmuCI GTSAC 4 cut(s) 1048, 2129, 2170, 2440
NruI TCGCGA 1 cut(s) 465
NsiI ATGCAT 1 cut(s) 2920
NspI RCATGY 2 cut(s) 2918, 3233
NspV TTCGAA 3 cut(s) 573, 608, 2858
PaeI GCATGC 1 cut(s) 2918
PaeR7I CTCGAG 1 cut(s) 1625
PaqCI CACCTGC 2 cut(s) 46, 692
PauI GCGCGC 1 cut(s) 2292
PceI AGGCCT 1 cut(s) 774
PciI ACATGT 1 cut(s) 3229
PciSI GCTCTTC 4 cut(s) 216, 1541, 2936, 3334
PcsI WCGNNNNNNNCGW 1 cut(s) 2847
PctI GAATGC 2 cut(s) 825, 2504
PdiI GCCGGC 2 cut(s) 2305, 2739
PdmI GAANNNNTTC 2 cut(s) 604, 1074
PflFI GACNNNGTC 2 cut(s) 2378, 2395
PfoI TCCNGGA 2 cut(s) 218, 513
PinAI ACCGGT 1 cut(s) 938
PleI GAGTC 5 cut(s) 825, 990, 1189, 1323, 2177
PpsI GAGTC 5 cut(s) 825, 990, 1189, 1323, 2177
PpuMI RGGWCCY 1 cut(s) 2783
PscI ACATGT 1 cut(s) 3229
PshAI GACNNNNGTC 2 cut(s) 279, 407
PshBI ATTAAT 1 cut(s) 2534
Psp124BI GAGCTC 2 cut(s) 969, 2633
Psp1406I AACGTT 1 cut(s) 2772
Psp5II RGGWCCY 1 cut(s) 2783
Psp6I CCWGG 5 cut(s) 218, 1669, 2342, 2785, 3305
PspFI CCCAGC 1 cut(s) 2331
PspGI CCWGG 5 cut(s) 218, 1669, 2342, 2785, 3305
PspN4I GGNNCC 6 cut(s) 8, 241, 2348, 2413, 2784, 2907
PspPI GGNCC 5 cut(s) 202, 362, 869, 941, 2783
PspPPI RGGWCCY 1 cut(s) 2783
PspXI VCTCGAGB 1 cut(s) 1625
PstI CTGCAG 6 cut(s) 311, 899, 909, 1043, 1695, 3138
PstNI CAGNNNCTG 3 cut(s) 913, 985, 1520
PsuI RGATCY 2 cut(s) 1815, 3107
PsyI GACNNNGTC 2 cut(s) 2378, 2395
PteI GCGCGC 1 cut(s) 2292
PvuII CAGCTG 2 cut(s) 291, 2331
RruI TCGCGA 1 cut(s) 465
RseI CAYNNNNRTG 3 cut(s) 1055, 1647, 2754
SacI GAGCTC 2 cut(s) 969, 2633
SapI GCTCTTC 4 cut(s) 216, 1541, 2936, 3334
SaqAI TTAA 9 cut(s) 233, 398, 1265, 1490, 1553, 1754, 2024, 2534, 3263
Sau96I GGNCC 5 cut(s) 202, 362, 869, 941, 2783
ScaI AGTACT 1 cut(s) 2259
SchI GAGTC 5 cut(s) 825, 991, 1189, 1324, 2178
ScrFI CCNGG 7 cut(s) 220, 515, 1671, 2344, 2787, 3018, 3307
SduI GDGCHC 3 cut(s) 969, 2633, 3028
SexAI ACCWGGT 1 cut(s) 2342
SfaNI GCATC 5 cut(s) 1812, 2913, 2927, 3004, 3302
SfcI CTRYAG 6 cut(s) 307, 895, 905, 1039, 1691, 3134
Sfr274I CTCGAG 1 cut(s) 1625
SfuI TTCGAA 3 cut(s) 573, 608, 2858
SinI GGWCC 4 cut(s) 362, 869, 941, 2783
SlaI CTCGAG 1 cut(s) 1625
SmiMI CAYNNNNRTG 3 cut(s) 1055, 1647, 2754
SmlI CTYRAG 5 cut(s) 389, 1256, 1625, 2626, 3215
SmoI CTYRAG 5 cut(s) 389, 1256, 1625, 2626, 3215
SpeI ACTAGT 1 cut(s) 2284
SphI GCATGC 1 cut(s) 2918
SseBI AGGCCT 1 cut(s) 774
SsiI CCGC 6 cut(s) 648, 1454, 1579, 2166, 2290, 2557
SspMI CTAG 7 cut(s) 686, 1844, 2285, 2465, 2676, 2823, 3143
SstI GAGCTC 2 cut(s) 969, 2633
StuI AGGCCT 1 cut(s) 774
StyD4I CCNGG 7 cut(s) 218, 513, 1669, 2342, 2785, 3016, 3305
StyI CCWWGG 1 cut(s) 734
TaaI ACNGT 7 cut(s) 841, 1054, 1328, 1522, 2150, 2843, 2957
TaiI ACGT 2 cut(s) 2775, 3187
TatI WGTACW 5 cut(s) 849, 915, 1482, 2257, 2952
TauI GCSGC 1 cut(s) 651
Tru1I TTAA 9 cut(s) 233, 398, 1265, 1490, 1553, 1754, 2024, 2534, 3263
Tru9I TTAA 9 cut(s) 233, 398, 1265, 1490, 1553, 1754, 2024, 2534, 3263
TscAI CASTG 6 cut(s) 436, 493, 1192, 2257, 2475, 2941
TseFI GTSAC 4 cut(s) 1048, 2129, 2170, 2440
Tsp45I GTSAC 4 cut(s) 1048, 2129, 2170, 2440
TspDTI ATGAA 4 cut(s) 2234, 2783, 2880, 3209
TspGWI ACGGA 1 cut(s) 260
TspRI CASTG 6 cut(s) 436, 493, 1192, 2257, 2475, 2941
Tth111I GACNNNGTC 2 cut(s) 2378, 2395
VneI GTGCAC 1 cut(s) 3024
VpaK11BI GGWCC 4 cut(s) 362, 869, 941, 2783
VspI ATTAAT 1 cut(s) 2534
XceI RCATGY 2 cut(s) 2918, 3233
XcmI CCANNNNNNNNNTGG 1 cut(s) 322
XhoI CTCGAG 1 cut(s) 1625
XmiI GTMKAC 3 cut(s) 2277, 2433, 3234
XmnI GAANNNNTTC 2 cut(s) 604, 1074
XspI CTAG 7 cut(s) 686, 1844, 2285, 2465, 2676, 2823, 3143
ZrmI AGTACT 1 cut(s) 2259
Zsp2I ATGCAT 1 cut(s) 2920
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.