pycom03g02840
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0008954
Physical Location & Seq
Forward (+)
2226989 .. 2228003
1015 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g02840.2

Sequence Viewer

Length: 936 bp
ATGTGGTCATCAGAAGAGTCAGGAGCATTCAATTCCGAAATTCAGACGCTCGGATCAATCAGGCACAAGAACATCATCCGGCTCTTGGGTTGGGGTTCAAACCGAAACCTGAAAATTCTGTTCTATGATTACCTTCCCAGCGGAAGCTTGAGCTCACTTCTACATGGTTACGGGAAAGGAGGAGCAGATTGGGAGGCTAGATACGATATTGTTTTAGGCGTGGCTCATGCACTTGCGTACTTGCACCATGACTGTGTGCCGGCTATTCTGCATGGTGATGTGAAAGCCATGAATGTTCTGTTGGGTCCTGGCCATGAGCCTTATCTTGCTGACTTTGGATTAGCTAGGATTGTGAACAGCAATGGTGGTGATGAATTTTCGAAAACAAGTCATCGGCCTCAATTGGCTGGTTCGTATGGATACATGGCTCCTGGTAATGTTTACTTTCAACGAAGTTTAGTTAAGCATGCATCAATGCAAAGTATCACTGAGAAGAGTGATGTCTACAGTTTTGGCGTTGTTCTTTTAGAGGTACTAACAGGAAGGCATCCGTTAGACCCAGCTCTGCCCGGAGGTGCACATTTAGTTCAGTGGATTCGGGACCACTTAGCGTGCAAGAGAGACCCGATTGACATTCTCGATCGAAATCTCAGTGGAAGATCTGATCCTGCAATGCACGAAATGCTGCAGACACTAGCTGTGTCATTTCTCTGTGTCAGCAACCGTGCTGATGAACGCCCGATGATGAAGGATGTCGTTGCAATGCTCAAGGAAATTCGACATGTAGACTGCGCAAGGCCGGAACCGGAGTTGTTAAAGGGAGGAGGTTTGCAAGCAATTCTGGCATCCCTTCCTGCTAGGAAGGGAGTGTCACAAGGCTCACAAGGCTCTTCTAACTACTCCTTTGCCTTCTCTGAAGATTCAAGGTCTCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_1 LRR_8 Pkinase

Protein Analysis

312

Amino Acids

34.05

Weight (kDa)

6.7

Isoelectric Point (pI)

47.0

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 793
AccI GTMKAC 2 cut(s) 504, 786
AciI CCGC 1 cut(s) 141
AclWI GGATC 2 cut(s) 61, 659
AcoI YGGCCR 1 cut(s) 310
AcsI RAATTY 4 cut(s) 39, 114, 374, 774
AcuI CTGAAG 1 cut(s) 936
AfaI GTAC 2 cut(s) 239, 534
AfiI CCNNNNNNNGG 1 cut(s) 85
AflIII ACRYGT 1 cut(s) 781
AgsI TTSAA 4 cut(s) 31, 99, 449, 924
AjnI CCWGG 2 cut(s) 307, 430
AjuI GAANNNNNNNTTGG 2 cut(s) 284, 316
AluBI AGCT 5 cut(s) 147, 153, 344, 563, 698
AluI AGCT 5 cut(s) 147, 153, 344, 563, 698
Alw21I GWGCWC 2 cut(s) 155, 580
Alw26I GTCTC 1 cut(s) 615
Alw44I GTGCAC 1 cut(s) 576
AlwI GGATC 2 cut(s) 61, 659
AoxI GGCC 3 cut(s) 310, 395, 797
ApaLI GTGCAC 1 cut(s) 576
ApeKI GCWGC 1 cut(s) 685
ApoI RAATTY 4 cut(s) 39, 114, 374, 774
AspLEI GCGC 1 cut(s) 794
AspS9I GGNCC 2 cut(s) 305, 601
AsuC2I CCSGG 1 cut(s) 570
AsuHPI GGTGA 2 cut(s) 287, 380
AsuII TTCGAA 1 cut(s) 380
AvaII GGWCC 2 cut(s) 305, 601
BaeGI GKGCMC 1 cut(s) 580
BalI TGGCCA 1 cut(s) 312
BanII GRGCYC 1 cut(s) 155
Bbv12I GWGCWC 2 cut(s) 155, 580
BbvI GCAGC 1 cut(s) 672
BciT130I CCWGG 2 cut(s) 309, 432
BciVI GTATCC 1 cut(s) 413
BcnI CCSGG 1 cut(s) 570
BcoDI GTCTC 1 cut(s) 615
BfaI CTAG 4 cut(s) 198, 345, 695, 858
BfmI CTRYAG 2 cut(s) 505, 686
BfuI GTATCC 1 cut(s) 413
BglII AGATCT 1 cut(s) 659
BisI GCNGC 1 cut(s) 686
BlsI GCNGC 1 cut(s) 687
Bme1390I CCNGG 3 cut(s) 309, 432, 570
Bme18I GGWCC 2 cut(s) 305, 601
BmgT120I GGNCC 2 cut(s) 305, 601
BmiI GGNNCC 4 cut(s) 306, 429, 602, 804
BmrFI CCNGG 3 cut(s) 309, 432, 570
BmsI GCATC 3 cut(s) 479, 556, 854
Bpu14I TTCGAA 1 cut(s) 380
BpuEI CTTGAG 2 cut(s) 169, 752
BpuMI CCSGG 1 cut(s) 570
BsaBI GATNNNNATC 1 cut(s) 645
BsaI GGTCTC 1 cut(s) 615
BsaWI WCCGGW 1 cut(s) 805
Bsc4I CCNNNNNNNGG 1 cut(s) 85
Bse118I RCCGGY 1 cut(s) 259
Bse3DI GCAATG 3 cut(s) 367, 678, 768
Bse8I GATNNNNATC 1 cut(s) 645
BseBI CCWGG 2 cut(s) 309, 432
BseGI GGATG 4 cut(s) 75, 547, 757, 845
BseJI GATNNNNATC 1 cut(s) 645
BseLI CCNNNNNNNGG 1 cut(s) 85
BseMI GCAATG 3 cut(s) 367, 678, 768
BseMII CTCAG 2 cut(s) 480, 664
BseRI GAGGAG 2 cut(s) 195, 837
BseSI GKGCMC 1 cut(s) 580
BseXI GCAGC 1 cut(s) 672
BseYI CCCAGC 2 cut(s) 137, 559
Bsh1285I CGRYCG 1 cut(s) 643
BshFI GGCC 3 cut(s) 312, 397, 799
BsiEI CGRYCG 1 cut(s) 643
BsiHKAI GWGCWC 2 cut(s) 155, 580
BsiSI CCGG 5 cut(s) 79, 260, 570, 800, 806
BslFI GGGAC 1 cut(s) 614
BslI CCNNNNNNNGG 1 cut(s) 85
BsmAI GTCTC 1 cut(s) 615
BsmFI GGGAC 1 cut(s) 614
BsmI GAATGC 1 cut(s) 26
BsnI GGCC 3 cut(s) 312, 397, 799
Bso31I GGTCTC 1 cut(s) 615
Bsp119I TTCGAA 1 cut(s) 380
Bsp1286I GDGCHC 2 cut(s) 155, 580
Bsp143I GATC 4 cut(s) 53, 640, 659, 664
BspACI CCGC 1 cut(s) 141
BspANI GGCC 3 cut(s) 312, 397, 799
BspCNI CTCAG 2 cut(s) 481, 663
BspLI GGNNCC 4 cut(s) 306, 429, 602, 804
BspMAI CTGCAG 1 cut(s) 690
BspPI GGATC 2 cut(s) 61, 659
BspQI GCTCTTC 1 cut(s) 895
BspT104I TTCGAA 1 cut(s) 380
BspTNI GGTCTC 1 cut(s) 615
BsrDI GCAATG 3 cut(s) 367, 678, 768
BsrFI RCCGGY 1 cut(s) 259
BssAI RCCGGY 1 cut(s) 259
BssMI GATC 4 cut(s) 53, 640, 659, 664
Bst2UI CCWGG 2 cut(s) 309, 432
Bst4CI ACNGT 3 cut(s) 254, 509, 725
Bst6I CTCTTC 3 cut(s) 9, 488, 895
BstAPI GCANNNNNTGC 1 cut(s) 682
BstBI TTCGAA 1 cut(s) 380
BstC8I GCNNGC 4 cut(s) 261, 468, 613, 834
BstDEI CTNAG 3 cut(s) 489, 607, 650
BstF5I GGATG 4 cut(s) 75, 547, 757, 845
BstHHI GCGC 1 cut(s) 794
BstKTI GATC 4 cut(s) 56, 643, 662, 667
BstMAI GTCTC 1 cut(s) 615
BstMBI GATC 4 cut(s) 53, 640, 659, 664
BstMCI CGRYCG 1 cut(s) 643
BstMWI GCNNNNNNNGC 3 cut(s) 682, 842, 885
BstNI CCWGG 2 cut(s) 309, 432
BstNSI RCATGY 2 cut(s) 470, 785
BstSCI CCNGG 3 cut(s) 307, 430, 568
BstSFI CTRYAG 2 cut(s) 505, 686
BstSLI GKGCMC 1 cut(s) 580
BstV1I GCAGC 1 cut(s) 672
BstX2I RGATCY 1 cut(s) 659
BstYI RGATCY 1 cut(s) 659
BsuI GTATCC 1 cut(s) 413
BsuRI GGCC 3 cut(s) 312, 397, 799
BtsCI GGATG 4 cut(s) 75, 547, 757, 845
BtsIMutI CAGTG 3 cut(s) 486, 596, 658
Cac8I GCNNGC 4 cut(s) 261, 468, 613, 834
CfoI GCGC 1 cut(s) 794
Cfr10I RCCGGY 1 cut(s) 259
Cfr13I GGNCC 2 cut(s) 305, 601
CseI GACGC 1 cut(s) 55
Csp6I GTAC 2 cut(s) 238, 533
CviAII CATG 9 cut(s) 164, 227, 248, 272, 289, 314, 424, 467, 782
CviQI GTAC 2 cut(s) 238, 533
DdeI CTNAG 3 cut(s) 489, 607, 650
DpnI GATC 4 cut(s) 55, 642, 661, 666
DpnII GATC 4 cut(s) 53, 640, 659, 664
EaeI YGGCCR 1 cut(s) 310
Eam1104I CTCTTC 3 cut(s) 9, 488, 895
EarI CTCTTC 3 cut(s) 9, 488, 895
Ecl136II GAGCTC 1 cut(s) 153
Eco24I GRGCYC 1 cut(s) 155
Eco31I GGTCTC 1 cut(s) 615
Eco47I GGWCC 2 cut(s) 305, 601
Eco53kI GAGCTC 1 cut(s) 153
Eco57I CTGAAG 1 cut(s) 936
EcoICRI GAGCTC 1 cut(s) 153
EcoO109I RGGNCCY 1 cut(s) 305
EcoRII CCWGG 2 cut(s) 307, 430
EcoT22I ATGCAT 1 cut(s) 472
EcoT38I GRGCYC 1 cut(s) 155
FaeI CATG 9 cut(s) 167, 230, 251, 275, 292, 317, 427, 470, 785
FaqI GGGAC 1 cut(s) 614
FatI CATG 9 cut(s) 163, 226, 247, 271, 288, 313, 423, 466, 781
FblI GTMKAC 2 cut(s) 504, 786
Fnu4HI GCNGC 1 cut(s) 686
FokI GGATG 4 cut(s) 62, 534, 764, 832
FriOI GRGCYC 1 cut(s) 155
Fsp4HI GCNGC 1 cut(s) 686
FspBI CTAG 4 cut(s) 198, 345, 695, 858
FspI TGCGCA 1 cut(s) 793
GlaI GCGC 1 cut(s) 793
GluI GCNGC 1 cut(s) 686
GsaI CCCAGC 2 cut(s) 141, 563
HaeIII GGCC 3 cut(s) 312, 397, 799
HapII CCGG 5 cut(s) 79, 260, 570, 800, 806
HgaI GACGC 1 cut(s) 55
HhaI GCGC 1 cut(s) 794
Hin1II CATG 9 cut(s) 167, 230, 251, 275, 292, 317, 427, 470, 785
Hin6I GCGC 1 cut(s) 792
HinP1I GCGC 1 cut(s) 792
HindIII AAGCTT 1 cut(s) 145
HinfI GANTC 3 cut(s) 17, 595, 920
HpaII CCGG 5 cut(s) 79, 260, 570, 800, 806
HphI GGTGA 2 cut(s) 287, 380
Hpy166II GTNNAC 5 cut(s) 355, 442, 505, 578, 787
Hpy188I TCNGA 6 cut(s) 13, 37, 45, 53, 664, 916
Hpy188III TCNNGA 3 cut(s) 21, 599, 638
Hpy8I GTNNAC 5 cut(s) 355, 442, 505, 578, 787
HpyAV CCTTC 6 cut(s) 143, 537, 742, 856, 860, 919
HpyCH4III ACNGT 3 cut(s) 254, 509, 725
HpyF10VI GCNNNNNNNGC 3 cut(s) 682, 842, 885
HpyF3I CTNAG 3 cut(s) 489, 607, 650
Hsp92II CATG 9 cut(s) 167, 230, 251, 275, 292, 317, 427, 470, 785
HspAI GCGC 1 cut(s) 792
KroI GCCGGC 1 cut(s) 259
KroNI GCCGGC 1 cut(s) 261
Kzo9I GATC 4 cut(s) 53, 640, 659, 664
LguI GCTCTTC 1 cut(s) 895
LmnI GCTCC 3 cut(s) 23, 182, 433
Lsp1109I GCAGC 1 cut(s) 672
LweI GCATC 3 cut(s) 479, 556, 854
MaeI CTAG 4 cut(s) 198, 345, 695, 858
MaeIII GTNAC 2 cut(s) 167, 870
MalI GATC 4 cut(s) 55, 642, 661, 666
MboI GATC 4 cut(s) 53, 640, 659, 664
MboII GAAGA 5 cut(s) 26, 505, 669, 882, 929
MfeI CAATTG 1 cut(s) 401
MflI RGATCY 1 cut(s) 659
MhlI GDGCHC 2 cut(s) 155, 580
MlsI TGGCCA 1 cut(s) 312
MluCI AATT 7 cut(s) 31, 39, 114, 374, 401, 774, 837
MluNI TGGCCA 1 cut(s) 312
MlyI GAGTC 1 cut(s) 26
MnlI CCTC 7 cut(s) 173, 187, 408, 523, 566, 815, 818
Mox20I TGGCCA 1 cut(s) 312
Mph1103I ATGCAT 1 cut(s) 472
MroNI GCCGGC 1 cut(s) 259
MscI TGGCCA 1 cut(s) 312
MseI TTAA 2 cut(s) 462, 815
MslI CAYNNNNRTG 2 cut(s) 252, 276
Msp20I TGGCCA 1 cut(s) 312
MspA1I CMGCKG 1 cut(s) 141
MspI CCGG 5 cut(s) 79, 260, 570, 800, 806
MspR9I CCNGG 3 cut(s) 309, 432, 570
MunI CAATTG 1 cut(s) 401
Mva1269I GAATGC 1 cut(s) 26
MvaI CCWGG 2 cut(s) 309, 432
MwoI GCNNNNNNNGC 3 cut(s) 682, 842, 885
NaeI GCCGGC 1 cut(s) 261
NciI CCSGG 1 cut(s) 570
NdeII GATC 4 cut(s) 53, 640, 659, 664
NgoMIV GCCGGC 1 cut(s) 259
NlaIII CATG 9 cut(s) 167, 230, 251, 275, 292, 317, 427, 470, 785
NlaIV GGNNCC 4 cut(s) 306, 429, 602, 804
NmuCI GTSAC 1 cut(s) 870
NsbI TGCGCA 1 cut(s) 793
NsiI ATGCAT 1 cut(s) 472
NspI RCATGY 2 cut(s) 470, 785
NspV TTCGAA 1 cut(s) 380
PaeI GCATGC 1 cut(s) 470
PciI ACATGT 1 cut(s) 781
PciSI GCTCTTC 1 cut(s) 895
PctI GAATGC 1 cut(s) 26
PdiI GCCGGC 1 cut(s) 261
PfeI GAWTC 2 cut(s) 595, 920
PkrI GCNGC 1 cut(s) 687
Ple19I CGATCG 1 cut(s) 643
PleI GAGTC 1 cut(s) 25
PpsI GAGTC 1 cut(s) 25
PpuMI RGGWCCY 1 cut(s) 305
PscI ACATGT 1 cut(s) 781
Psp124BI GAGCTC 1 cut(s) 155
Psp5II RGGWCCY 1 cut(s) 305
Psp6I CCWGG 2 cut(s) 307, 430
PspFI CCCAGC 2 cut(s) 137, 559
PspGI CCWGG 2 cut(s) 307, 430
PspN4I GGNNCC 4 cut(s) 306, 429, 602, 804
PspPI GGNCC 2 cut(s) 305, 601
PspPPI RGGWCCY 1 cut(s) 305
PstI CTGCAG 1 cut(s) 690
PsuI RGATCY 1 cut(s) 659
PvuI CGATCG 1 cut(s) 643
RsaI GTAC 2 cut(s) 239, 534
RsaNI GTAC 2 cut(s) 238, 533
RseI CAYNNNNRTG 2 cut(s) 252, 276
SacI GAGCTC 1 cut(s) 155
SapI GCTCTTC 1 cut(s) 895
SaqAI TTAA 2 cut(s) 462, 815
SatI GCNGC 1 cut(s) 686
Sau3AI GATC 4 cut(s) 53, 640, 659, 664
Sau96I GGNCC 2 cut(s) 305, 601
SchI GAGTC 1 cut(s) 26
ScrFI CCNGG 3 cut(s) 309, 432, 570
SduI GDGCHC 2 cut(s) 155, 580
SfaNI GCATC 3 cut(s) 479, 556, 854
SfcI CTRYAG 2 cut(s) 505, 686
SfuI TTCGAA 1 cut(s) 380
SinI GGWCC 2 cut(s) 305, 601
SmiMI CAYNNNNRTG 2 cut(s) 252, 276
SmlI CTYRAG 2 cut(s) 148, 767
SmoI CTYRAG 2 cut(s) 148, 767
SphI GCATGC 1 cut(s) 470
Sse9I AATT 7 cut(s) 31, 39, 114, 374, 401, 774, 837
SsiI CCGC 1 cut(s) 141
SspMI CTAG 4 cut(s) 198, 345, 695, 858
SstI GAGCTC 1 cut(s) 155
StyD4I CCNGG 3 cut(s) 307, 430, 568
TaaI ACNGT 3 cut(s) 254, 509, 725
TaqI TCGA 4 cut(s) 380, 639, 643, 778
TasI AATT 7 cut(s) 31, 39, 114, 374, 401, 774, 837
TfiI GAWTC 2 cut(s) 595, 920
Tru1I TTAA 2 cut(s) 462, 815
Tru9I TTAA 2 cut(s) 462, 815
TscAI CASTG 3 cut(s) 493, 596, 658
TseFI GTSAC 1 cut(s) 870
TseI GCWGC 1 cut(s) 685
Tsp45I GTSAC 1 cut(s) 870
TspDTI ATGAA 4 cut(s) 305, 387, 747, 761
TspGWI ACGGA 1 cut(s) 540
TspRI CASTG 3 cut(s) 493, 596, 658
VneI GTGCAC 1 cut(s) 576
VpaK11BI GGWCC 2 cut(s) 305, 601
XapI RAATTY 4 cut(s) 39, 114, 374, 774
XceI RCATGY 2 cut(s) 470, 785
XmiI GTMKAC 2 cut(s) 504, 786
XspI CTAG 4 cut(s) 198, 345, 695, 858
Zsp2I ATGCAT 1 cut(s) 472
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.