MD03G1037200.v1.1

Plant protein of unknown function (DUF868)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0017083
Physical Location & Seq
Reverse (-)
2955483 .. 2957301
1819 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1037200.v1.1.491

Sequence Viewer

Length: 1053 bp
ATGTTGCAGTTCTCGATCGAGTTTTTCTTATACTGGAAGCTATTTTTAGGGTTTACAGGTGGAGTAGATAATGAGGCATCTCTTTTCCTCGTGACAATGTCTCATCAAATTCTTCAGGCCTCACCCGTTTCCATTCCAAAATCACCATCTTCCGAGAAAGTGTGCAAGGAAAAGGAGAAAGATAAGTCAGCTCCGCAGAGCGCTGTGACTTATGTCTATCAAGCGAAAGTAGCTGAATTGTGCTGCAATGTCACAGCGACATGGTGTAAAAACCTAACTAGCCATTCCCTCTGCATCACAGTGGAAAAGCCTTGTCAACAAAATCAGTGCTGTACTTGTAAGATAGACTTAAGCTCAACCCAGTTTTGGGGCAAGAAAGGTCTCAAATCGTTCGAAGTAGATGGAAGACGGGTGGATGTGTACTGGGATTTTCGACAGGCGAAATTCTCCTGCACCCCAGAGCCATGCTCGAATTACTATGTTGCCTTGGTGTCCAAACAAGAGGTGGTTTTACTGCTGGGGGACTTAATGGAAGATGCTTATAAGCGGACCAGATCAAGATCATCATCGGAAGAGGCTAGGTTGATGTACAAGAAGGAAAATGTGTGCGGGAAAGGATTGTTTTGCACCAAAGCCATGTTGGAGGAAGGGAGGAAAGAACATGACATTGTGATTGAAACGTTGTTGTCCGGGCCCAAAGATCCTGAAATGTGGATTAGCGTAGATGGGACAGTGGCAATTCGGATTATGAACTTGAATTGGAGGTTTAGAGGGAATAAAACTGTGATGCTGAATGATATGCCAGTTGAAATTTTTTGGGATGTGCATGATTGGTTGTTTAGTAGCTTAGGCACAAGCCAAGGGTTTTTCATTTTTAAGGCAGGGACGGTAGATTGGGAATCATCGAATATCAATTATGATTATGGGAGTAGAAATGGAAACTGCAGCAGTTGCAGCCTGCAGCACAGTCCATGTAACTCACCAAAAGGCAAAGGGAGCTCATCAAACATAGGAGGGTTTTGCCATTTTCTCTATGCTTGGAAAACCGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF868

Protein Analysis

351

Amino Acids

39.61

Weight (kDa)

7.8

Isoelectric Point (pI)

46.49

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF868 PF05910 65 - 348 1.6e-97 Plant protein of unknown function (DUF868)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017083)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G13229
fragaria_vesca FvH4_3g42170
malus_domestica MD03G1037200.v1.1 MD11G1038300.v1.1
pyrus_communis pycom03g02860
rosa_chinensis RchiOBHm_Chr5g0075431 RchiOBHm_Chr5g0075441
rosa_laevigata RLG00000036561
rosa_multiflora Rmu_sc0000770.1_g000016
rosa_rugosa Rorug05G0439500
rosa_samantha Rh5BG517800 Rh5CG541400
rosa_wichuraiana Rw5G046040

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 543
AciI CCGC 3 cut(s) 194, 547, 609
AclI AACGTT 1 cut(s) 680
AclWI GGATC 1 cut(s) 695
AcsI RAATTY 3 cut(s) 108, 443, 810
AcuI CTGAAG 1 cut(s) 98
AfaI GTAC 3 cut(s) 334, 422, 590
AfeI AGCGCT 1 cut(s) 202
AfiI CCNNNNNNNGG 2 cut(s) 366, 367
AflII CTTAAG 1 cut(s) 349
AgsI TTSAA 3 cut(s) 677, 757, 809
AluBI AGCT 6 cut(s) 40, 191, 233, 354, 846, 999
AluI AGCT 6 cut(s) 40, 191, 233, 354, 846, 999
Alw21I GWGCWC 1 cut(s) 1001
Alw26I GTCTC 2 cut(s) 105, 386
AlwI GGATC 1 cut(s) 695
Aor51HI AGCGCT 1 cut(s) 202
AoxI GGCC 2 cut(s) 117, 692
ApaI GGGCCC 1 cut(s) 696
ApeKI GCWGC 4 cut(s) 243, 945, 954, 961
ApoI RAATTY 3 cut(s) 108, 443, 810
AspLEI GCGC 1 cut(s) 203
AspS9I GGNCC 3 cut(s) 549, 692, 693
AsuC2I CCSGG 1 cut(s) 691
AsuHPI GGTGA 3 cut(s) 114, 135, 972
AsuII TTCGAA 1 cut(s) 393
AvaII GGWCC 1 cut(s) 549
BaeGI GKGCMC 1 cut(s) 696
BanII GRGCYC 2 cut(s) 696, 1001
BauI CACGAG 1 cut(s) 89
BbsI GAAGAC 1 cut(s) 412
Bbv12I GWGCWC 1 cut(s) 1001
BbvI GCAGC 4 cut(s) 230, 957, 966, 973
BccI CCATC 3 cut(s) 154, 395, 719
BcnI CCSGG 1 cut(s) 691
BcoDI GTCTC 2 cut(s) 105, 386
BfaI CTAG 2 cut(s) 279, 579
BfmI CTRYAG 2 cut(s) 943, 959
BfoI RGCGCY 1 cut(s) 204
BfrI CTTAAG 1 cut(s) 349
BisI GCNGC 4 cut(s) 244, 946, 955, 962
BlsI GCNGC 4 cut(s) 245, 947, 956, 963
Bme1390I CCNGG 1 cut(s) 691
Bme18I GGWCC 1 cut(s) 549
BmgT120I GGNCC 3 cut(s) 549, 692, 693
BmiI GGNNCC 1 cut(s) 694
BmrFI CCNGG 1 cut(s) 691
BmrI ACTGGG 2 cut(s) 355, 433
BmsI GCATC 4 cut(s) 86, 303, 526, 777
BmuI ACTGGG 2 cut(s) 355, 433
BoxI GACNNNNGTC 1 cut(s) 212
BpiI GAAGAC 1 cut(s) 412
BplI GAGNNNNNCTC 2 cut(s) 452, 484
Bpu10I CCTNAGC 1 cut(s) 847
Bpu14I TTCGAA 1 cut(s) 393
BpuMI CCSGG 1 cut(s) 691
BsaBI GATNNNNATC 2 cut(s) 559, 565
BsaI GGTCTC 1 cut(s) 386
BsaJI CCNNGG 2 cut(s) 486, 859
Bsc4I CCNNNNNNNGG 2 cut(s) 366, 367
Bse1I ACTGG 4 cut(s) 38, 361, 428, 803
Bse3DI GCAATG 1 cut(s) 253
Bse8I GATNNNNATC 2 cut(s) 559, 565
BseDI CCNNGG 2 cut(s) 486, 859
BseGI GGATG 2 cut(s) 421, 826
BseJI GATNNNNATC 2 cut(s) 559, 565
BseLI CCNNNNNNNGG 2 cut(s) 366, 367
BseMI GCAATG 1 cut(s) 253
BseNI ACTGG 4 cut(s) 38, 361, 428, 803
BseSI GKGCMC 1 cut(s) 696
BseXI GCAGC 4 cut(s) 230, 957, 966, 973
BseYI CCCAGC 1 cut(s) 517
BsgI GTGCAG 1 cut(s) 436
Bsh1285I CGRYCG 1 cut(s) 18
BshFI GGCC 2 cut(s) 119, 694
BsiEI CGRYCG 1 cut(s) 18
BsiHKAI GWGCWC 1 cut(s) 1001
BsiSI CCGG 1 cut(s) 690
BslFI GGGAC 3 cut(s) 536, 742, 898
BslI CCNNNNNNNGG 2 cut(s) 366, 367
BsmAI GTCTC 2 cut(s) 105, 386
BsmFI GGGAC 3 cut(s) 536, 742, 898
BsnI GGCC 2 cut(s) 119, 694
Bso31I GGTCTC 1 cut(s) 386
Bsp119I TTCGAA 1 cut(s) 393
Bsp120I GGGCCC 1 cut(s) 692
Bsp1286I GDGCHC 2 cut(s) 696, 1001
Bsp1407I TGTACA 1 cut(s) 588
Bsp143I GATC 4 cut(s) 15, 554, 560, 700
BspACI CCGC 3 cut(s) 194, 547, 609
BspANI GGCC 2 cut(s) 119, 694
BspLI GGNNCC 1 cut(s) 694
BspMAI CTGCAG 2 cut(s) 947, 963
BspPI GGATC 1 cut(s) 695
BspT104I TTCGAA 1 cut(s) 393
BspTI CTTAAG 1 cut(s) 349
BspTNI GGTCTC 1 cut(s) 386
BsrDI GCAATG 1 cut(s) 253
BsrGI TGTACA 1 cut(s) 588
BsrI ACTGG 4 cut(s) 38, 361, 428, 803
BssECI CCNNGG 2 cut(s) 486, 859
BssMI GATC 4 cut(s) 15, 554, 560, 700
BssSI CACGAG 1 cut(s) 89
BssT1I CCWWGG 2 cut(s) 486, 859
Bst2BI CACGAG 1 cut(s) 89
Bst4CI ACNGT 5 cut(s) 301, 733, 784, 889, 968
Bst6I CTCTTC 1 cut(s) 567
BstAFI CTTAAG 1 cut(s) 349
BstAPI GCANNNNNTGC 1 cut(s) 951
BstAUI TGTACA 1 cut(s) 588
BstBI TTCGAA 1 cut(s) 393
BstC8I GCNNGC 1 cut(s) 959
BstDEI CTNAG 1 cut(s) 847
BstF5I GGATG 2 cut(s) 421, 826
BstH2I RGCGCY 1 cut(s) 204
BstHHI GCGC 1 cut(s) 203
BstKTI GATC 4 cut(s) 18, 557, 563, 703
BstMAI GTCTC 2 cut(s) 105, 386
BstMBI GATC 4 cut(s) 15, 554, 560, 700
BstMCI CGRYCG 1 cut(s) 18
BstMWI GCNNNNNNNGC 4 cut(s) 230, 951, 954, 996
BstPAI GACNNNNGTC 1 cut(s) 212
BstSCI CCNGG 1 cut(s) 689
BstSFI CTRYAG 2 cut(s) 943, 959
BstSLI GKGCMC 1 cut(s) 696
BstV1I GCAGC 4 cut(s) 230, 957, 966, 973
BstV2I GAAGAC 1 cut(s) 412
BstX2I RGATCY 1 cut(s) 700
BstYI RGATCY 1 cut(s) 700
BsuRI GGCC 2 cut(s) 119, 694
BtsCI GGATG 2 cut(s) 421, 826
BtsIMutI CAGTG 3 cut(s) 306, 332, 738
Cac8I GCNNGC 1 cut(s) 959
CfoI GCGC 1 cut(s) 203
Cfr13I GGNCC 3 cut(s) 549, 692, 693
Csp6I GTAC 3 cut(s) 333, 421, 589
CviAII CATG 6 cut(s) 261, 465, 637, 662, 827, 972
CviQI GTAC 3 cut(s) 333, 421, 589
DdeI CTNAG 1 cut(s) 847
DpnI GATC 4 cut(s) 17, 556, 562, 702
DpnII GATC 4 cut(s) 15, 554, 560, 700
Eam1104I CTCTTC 1 cut(s) 567
EarI CTCTTC 1 cut(s) 567
Ecl136II GAGCTC 1 cut(s) 999
Eco130I CCWWGG 2 cut(s) 486, 859
Eco147I AGGCCT 1 cut(s) 119
Eco24I GRGCYC 2 cut(s) 696, 1001
Eco31I GGTCTC 1 cut(s) 386
Eco47I GGWCC 1 cut(s) 549
Eco47III AGCGCT 1 cut(s) 202
Eco53kI GAGCTC 1 cut(s) 999
Eco57I CTGAAG 1 cut(s) 98
EcoICRI GAGCTC 1 cut(s) 999
EcoT14I CCWWGG 2 cut(s) 486, 859
EcoT38I GRGCYC 2 cut(s) 696, 1001
ErhI CCWWGG 2 cut(s) 486, 859
FaeI CATG 6 cut(s) 264, 468, 640, 665, 830, 975
FalI AAGNNNNNCTT 2 cut(s) 332, 364
FaqI GGGAC 3 cut(s) 536, 742, 898
FatI CATG 6 cut(s) 260, 464, 636, 661, 826, 971
FauI CCCGC 1 cut(s) 602
Fnu4HI GCNGC 4 cut(s) 244, 946, 955, 962
FokI GGATG 2 cut(s) 428, 833
FriOI GRGCYC 2 cut(s) 696, 1001
Fsp4HI GCNGC 4 cut(s) 244, 946, 955, 962
FspBI CTAG 2 cut(s) 279, 579
GlaI GCGC 1 cut(s) 202
GluI GCNGC 4 cut(s) 244, 946, 955, 962
GsaI CCCAGC 1 cut(s) 521
HaeII RGCGCY 1 cut(s) 204
HaeIII GGCC 2 cut(s) 119, 694
HapII CCGG 1 cut(s) 690
HhaI GCGC 1 cut(s) 203
Hin1II CATG 6 cut(s) 264, 468, 640, 665, 830, 975
Hin6I GCGC 1 cut(s) 201
HinP1I GCGC 1 cut(s) 201
HincII GTYRAC 1 cut(s) 317
HindII GTYRAC 1 cut(s) 317
HinfI GANTC 1 cut(s) 899
HpaII CCGG 1 cut(s) 690
HphI GGTGA 3 cut(s) 114, 135, 972
Hpy166II GTNNAC 3 cut(s) 54, 317, 421
Hpy188I TCNGA 3 cut(s) 154, 571, 744
Hpy188III TCNNGA 4 cut(s) 13, 91, 558, 704
Hpy8I GTNNAC 3 cut(s) 54, 317, 421
HpyAV CCTTC 2 cut(s) 589, 641
HpyCH4III ACNGT 5 cut(s) 301, 733, 784, 889, 968
HpyCH4IV ACGT 1 cut(s) 680
HpyF10VI GCNNNNNNNGC 4 cut(s) 230, 951, 954, 996
HpyF3I CTNAG 1 cut(s) 847
HpySE526I ACGT 1 cut(s) 680
Hsp92II CATG 6 cut(s) 264, 468, 640, 665, 830, 975
HspAI GCGC 1 cut(s) 201
Kzo9I GATC 4 cut(s) 15, 554, 560, 700
LmnI GCTCC 2 cut(s) 196, 996
Lsp1109I GCAGC 4 cut(s) 230, 957, 966, 973
LweI GCATC 4 cut(s) 86, 303, 526, 777
MaeI CTAG 2 cut(s) 279, 579
MaeII ACGT 1 cut(s) 680
MaeIII GTNAC 4 cut(s) 91, 205, 250, 974
MalI GATC 4 cut(s) 17, 556, 562, 702
MboI GATC 4 cut(s) 15, 554, 560, 700
MboII GAAGA 5 cut(s) 104, 141, 417, 545, 584
MflI RGATCY 1 cut(s) 700
MhlI GDGCHC 2 cut(s) 696, 1001
MluCI AATT 8 cut(s) 108, 236, 443, 472, 738, 757, 810, 913
MmeI TCCRAC 1 cut(s) 621
MseI TTAA 3 cut(s) 350, 527, 876
MslI CAYNNNNRTG 1 cut(s) 299
MspCI CTTAAG 1 cut(s) 349
MspI CCGG 1 cut(s) 690
MspR9I CCNGG 1 cut(s) 691
MwoI GCNNNNNNNGC 4 cut(s) 230, 951, 954, 996
NciI CCSGG 1 cut(s) 691
NdeII GATC 4 cut(s) 15, 554, 560, 700
NlaIII CATG 6 cut(s) 264, 468, 640, 665, 830, 975
NlaIV GGNNCC 1 cut(s) 694
NmuCI GTSAC 3 cut(s) 91, 205, 250
NspV TTCGAA 1 cut(s) 393
PceI AGGCCT 1 cut(s) 119
PfeI GAWTC 1 cut(s) 899
PflFI GACNNNGTC 1 cut(s) 97
PkrI GCNGC 4 cut(s) 245, 947, 956, 963
Ple19I CGATCG 1 cut(s) 18
PshAI GACNNNNGTC 1 cut(s) 212
PsiI TTATAA 1 cut(s) 543
Psp124BI GAGCTC 1 cut(s) 1001
Psp1406I AACGTT 1 cut(s) 680
PspFI CCCAGC 1 cut(s) 517
PspN4I GGNNCC 1 cut(s) 694
PspOMI GGGCCC 1 cut(s) 692
PspPI GGNCC 3 cut(s) 549, 692, 693
PstI CTGCAG 2 cut(s) 947, 963
PsuI RGATCY 1 cut(s) 700
PsyI GACNNNGTC 1 cut(s) 97
PvuI CGATCG 1 cut(s) 18
RsaI GTAC 3 cut(s) 334, 422, 590
RsaNI GTAC 3 cut(s) 333, 421, 589
RseI CAYNNNNRTG 1 cut(s) 299
SacI GAGCTC 1 cut(s) 1001
SaqAI TTAA 3 cut(s) 350, 527, 876
SatI GCNGC 4 cut(s) 244, 946, 955, 962
Sau3AI GATC 4 cut(s) 15, 554, 560, 700
Sau96I GGNCC 3 cut(s) 549, 692, 693
ScrFI CCNGG 1 cut(s) 691
SduI GDGCHC 2 cut(s) 696, 1001
SfaNI GCATC 4 cut(s) 86, 303, 526, 777
SfcI CTRYAG 2 cut(s) 943, 959
SfuI TTCGAA 1 cut(s) 393
SinI GGWCC 1 cut(s) 549
SmiMI CAYNNNNRTG 1 cut(s) 299
SmlI CTYRAG 1 cut(s) 349
SmoI CTYRAG 1 cut(s) 349
Sse9I AATT 8 cut(s) 108, 236, 443, 472, 738, 757, 810, 913
SseBI AGGCCT 1 cut(s) 119
SsiI CCGC 3 cut(s) 194, 547, 609
SspMI CTAG 2 cut(s) 279, 579
SstI GAGCTC 1 cut(s) 1001
StuI AGGCCT 1 cut(s) 119
StyD4I CCNGG 1 cut(s) 689
StyI CCWWGG 2 cut(s) 486, 859
TaaI ACNGT 5 cut(s) 301, 733, 784, 889, 968
TaiI ACGT 1 cut(s) 683
TaqI TCGA 6 cut(s) 14, 18, 393, 433, 470, 905
TasI AATT 8 cut(s) 108, 236, 443, 472, 738, 757, 810, 913
TatI WGTACW 3 cut(s) 332, 420, 588
TfiI GAWTC 1 cut(s) 899
Tru1I TTAA 3 cut(s) 350, 527, 876
Tru9I TTAA 3 cut(s) 350, 527, 876
TscAI CASTG 3 cut(s) 306, 332, 738
TseFI GTSAC 3 cut(s) 91, 205, 250
TseI GCWGC 4 cut(s) 243, 945, 954, 961
Tsp45I GTSAC 3 cut(s) 91, 205, 250
TspDTI ATGAA 2 cut(s) 764, 859
TspRI CASTG 3 cut(s) 306, 332, 738
Tth111I GACNNNGTC 1 cut(s) 97
Vha464I CTTAAG 1 cut(s) 349
VpaK11BI GGWCC 1 cut(s) 549
XapI RAATTY 3 cut(s) 108, 443, 810
XcmI CCANNNNNNNNNTGG 2 cut(s) 502, 637
XspI CTAG 2 cut(s) 279, 579
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.