Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0006399

Physical Location & Seq

Genomic Coordinates Forward (+)

4125435 .. 4131269

Gene Structure 5835 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1051600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2658 bp

ATGGACTCCAAGATTTGCATGAATCCTTCGTGTAGGACGGCCAATACGCACGAATGGAAGAAAGGTTGGCCTTTGCGAACTGGTGGATTTGCCCATCTCTGCTACAAGTGCGGAGTTGAGTATGAGAATTCAGTTTACTGCAATACGTTCCATTCTGGGGAAACTGGTTGGAGGGACTGCAATTTGTGCCACAAGCCTCTCCACTGTGGATGCATAGTCTCAGAGACATTGTATGAGTGCCTGGATTTTGGGGGTATAGGGTGTATTGGCTGTGCTTCCCAACCTCGTGTGATCCAGAATGATGATGTGTTAAATGGATTTGGTGGATTGAAAATAAGTAATTCCGGTGATCAGCAATCTACAGTTCCTCAGAATGGAGCATTAAGTGATACTGCCAACGAGGGAAAACTTTTGCAATTGTGCCAAGTCATGGAGGCCAATGAATCCAACCTTTCGTCTCAATCTCAGAGAGGTGATATAAATGTATCTCCTGTGCAAAGCAAACAAGAAGAAGTTACTTATCCTAAGGGGGAAGTGGGCCCAGGATTTTCAAGTATCACTAGACCATCTATTGGATCATTAACATTTGCCAAACCAGACAATGGTAGAATAATGATAGAGGTCAAAGATATGAATAATCCATCTTCTGAGCCGTCTTTGAGTATGACTTTGGGAGGCCCATCTGCAACTCCAAATTTTGCTCAACCCTTTACTGGGGGAATTTTGGAGGGAAGAGATAAGAGCAAGATGCCTTCCTCCTTCCAGCAGGGGCAAAAAATTCGGCCTATTTTGCCCAGACCCTTGAGACCTCCCTCTCCTGTTCACCATGTGCGTGTTGCAAGGCCACCTTCTGAAGGGCGAGGGAAGAGTCAGTTACTTCCCCGATATTGGCCAAGGATTACTGACCAAGAGCTACAGAAATTAGCTGGAGATTTGAATTCTACTATTGTGCCACTGTTCGAGAAGGTGCTAAGTGCCAGTGATGCAGGTCGAATTGGTCGTCTGGTTCTCCCAAAAGCATGTGCTGAGGCATACTTTCCTCCTATTTCTCAATCAGAAGGCCTTCCTATAAGGATTCAAGATGTCAAGGGTAATGAATGGACGTTTCAGTTCAGATTTTGGCCAAATAATAATAGCAGGATGTATGTTTTAGAAGGAGTAACGCCTTGCATACAGTCTATGCAACTTCAAGCTGGTGATACCGTGACATTCAGTCGGATAGATCCTGGAGGCAGACTTGTAATTGGGTTCCGAAAGGCATCAAGAAGTTTAGATATGCAGGACCCCCAAACATCTTTCCTTCCCAATGGAACTCCTGGGGAAACTTCCTGTCCTAGTGTCGTTGAGAATCTAGTAACAGGAAGTGGTCAGTCTAGTATTTATCAAACGAATACAGGAAGCAAAGACCCTTACCTAAATGCTTTATCGGGACATTTGCATTTGGCTGAAGGGGATACGAGTTTGCATAATTGTGAGAACCATAGTCACTTAACAAGTGAGGATTTGCTGCAGCGGCCAGTGTCAAATTCTGACAAGAAGAGGGCCCGAAATATTGGGCCTAAAAGTAAGAGGTTGCTCTTGCATAGAGAAGATGTTCTAGAGCTGAGACTGACATGGGAAGAAGCACAGGACTTGCTTCGTCCACCCCCGAGTGTCAAACCAAGCATTGTAACTATAGAAGATCATGAATTCGAAGAGTATGATGAGCCTCCTGTTTTTGGGAAGAGATCTCTATTCATTGCCGAATCTGAGAGACAAGAACAATGGGCTCAGTGTGATGATTGCTTTAAGTGGCGGAGGTTGCCTGTCGACGTTTTTCTTCCTCCAAAGTGGACATGCTCTGAAAATTCTTGGGATATGAACAGGTGCTCATGTTCTGCACCAGAGGAGGTGAGCCAAAAGGAATTAGATAATCTTCTAAGACCAAGCATAGATTTAAAGAAGCGAAGAATCGTAGTAAACCATAATGAAGCCCAAGAACATGAACCTTCTGGCTTGGATGCCCTGGCGAGTGCTAGTGCTGCCATTCTGGGAGATAGTGTGGATTACTCTGGTGAGCAGTCACTTGGAGCCACAACCAGACATCCGCGTCATCGCCCAGGCTGCACTTGCATAGTATGTATTCAACCCCCGAGCGGGAAGGGCAAGCACAAGCCAACATGCACATGCAATGTGTGCATGACAGTAAAACGGCGCTTCAAAACCCTCATGATGAACAAGAGGAAACGCAAATCAGAACGTGAAGCGGAGAATGCCCAGAGGGATAATAATGACCACAAGGACGAATCAGAAATGAATGGCACATCAACAGAGATCGAGTTGCATAGGAATCACTCATCGGAGAATGGAGGCAGCAGCGAGAAGAGAATTGAAGCTGACGTGGCTGAAAGTTCATGCGCTGGACAAATTGATCTGAATTGTGAGCCCAAACACAATTATGTGCAGGTTTCTGGGTTGAACTTGTTGAGGCTTGCTGACGCTGTTGCTCGCCAACCAGTAAACAACGGCACAAGGGAAAGTTTCCTCACAAACAAAACGTGTGAGCCACAGGCTGGTCTTGGTTCCTCTTTACTTGCACAAGAGACTGCGGAGACTGACAGACTCATGTGTAATGAAGGCAGCCTTTCGTCCGTAGCAGCGTTGGACTTTGGAGGGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

B3 zf-CW

Protein Analysis

886

Amino Acids

97.64

Weight (kDa)

6.26

Isoelectric Point (pI)

53.1

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf_VAL1_N	PF25813	49 - 96	3.4e-22	VAL1-like, N-terminal zinc finger domain
B3	PF02362	320 - 420	9.4e-17	B3 DNA binding domain
zf-CW	PF07496	588 - 630	5.3e-11	CW-type Zinc Finger

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006399)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g40520 FvH4_3g40520 FvH4_3g40520 FvH4_3g40520 FvH4_3g40520 FvH4_3g40520 FvH4_3g40520 FvH4_3g40520
malus_domestica	MD03G1051600.v1.1 MD11G1053500.v1.1 MD11G1053700.v1.1
prunus_persica	Prupe.6G041000_v2.0.a1 Prupe.6G041000_v2.0.a1 Prupe.6G041000_v2.0.a1
pyrus_communis	pycom03g04100 pycom11g04330
rosa_chinensis	RchiOBHm_Chr5g0073201
rosa_laevigata	RLG00000036373
rosa_multiflora	Rmu_sc0001887.1_g000012
rosa_roxburghii	Rroxscaffold_1G00007990
rosa_rugosa	Rorug05G0422000 Rorug05G0422100
rosa_samantha	Rh5AG478900 Rh5BG499600 Rh5CG522800 Rh5DG511700
rosa_wichuraiana	Rw5G044520

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	2088
Acc36I	ACCTGC	2 cut(s)	977, 2434
AccB7I	CCANNNNNTGG	4 cut(s)	430, 572, 602, 2552
AccBSI	CCGCTC	1 cut(s)	2136
AccI	GTMKAC	1 cut(s)	1809
AccII	CGCG	1 cut(s)	2089
AciI	CCGC	7 cut(s)	111, 1513, 1795, 2087, 2136, 2246, 2588
AclWI	GGATC	3 cut(s)	286, 583, 1217
AcoI	YGGCCR	4 cut(s)	39, 890, 1121, 1514
AcsI	RAATTY	8 cut(s)	127, 694, 720, 777, 937, 1525, 1688, 1846
AcuI	CTGAAG	2 cut(s)	873, 1467
AdeI	CACNNNGTG	1 cut(s)	829
AflIII	ACRYGT	1 cut(s)	2537
AgsI	TTSAA	9 cut(s)	331, 552, 937, 1079, 1190, 2126, 2200, 2372, 2458
AhdI	GACNNNNNGTC	1 cut(s)	1212
AjiI	CACGTC	1 cut(s)	2380
AjnI	CCWGG	6 cut(s)	240, 541, 1225, 1315, 2004, 2098
AluBI	AGCT	5 cut(s)	913, 926, 1193, 1603, 2375
AluI	AGCT	5 cut(s)	913, 926, 1193, 1603, 2375
Alw21I	GWGCWC	1 cut(s)	1871
Alw26I	GTCTC	8 cut(s)	218, 223, 462, 799, 1600, 1747, 2576, 2585
AlwI	GGATC	3 cut(s)	286, 583, 1217
Ama87I	CYCGRG	2 cut(s)	1648, 2131
ApaI	GGGCCC	2 cut(s)	542, 1546
ApeKI	GCWGC	8 cut(s)	1507, 1510, 2021, 2103, 2352, 2355, 2619, 2636
ApoI	RAATTY	8 cut(s)	127, 694, 720, 777, 937, 1525, 1688, 1846
ArsI	GACNNNNNNTTYG	2 cut(s)	985, 1017
Asp700I	GAANNNNTTC	2 cut(s)	1062, 1593
AspLEI	GCGC	2 cut(s)	2196, 2399
AspS9I	GGNCC	7 cut(s)	538, 539, 677, 1282, 1542, 1543, 1556
AsuHPI	GGTGA	6 cut(s)	359, 485, 815, 1208, 1903, 2066
AsuII	TTCGAA	1 cut(s)	1692
AvaI	CYCGRG	2 cut(s)	1648, 2131
AvaII	GGWCC	1 cut(s)	1282
AxyI	CCTNAGG	1 cut(s)	525
BaeGI	GKGCMC	2 cut(s)	542, 1546
BalI	TGGCCA	2 cut(s)	892, 1123
BanII	GRGCYC	4 cut(s)	542, 1546, 1771, 2427
BauI	CACGAG	1 cut(s)	285
Bbv12I	GWGCWC	1 cut(s)	1871
BbvCI	CCTCAGC	1 cut(s)	1026
BbvI	GCAGC	8 cut(s)	1494, 1522, 2008, 2090, 2364, 2367, 2631, 2648
BccI	CCATC	4 cut(s)	102, 574, 649, 688
BceAI	ACGGC	4 cut(s)	54, 637, 2207, 2521
BcgI	CGANNNNNNTGC	2 cut(s)	761, 795
BciT130I	CCWGG	6 cut(s)	242, 543, 1227, 1317, 2006, 2100
BciVI	GTATCC	1 cut(s)	1447
BclI	TGATCA	1 cut(s)	349
BcoDI	GTCTC	8 cut(s)	218, 223, 462, 799, 1600, 1747, 2576, 2585
BfaI	CTAG	6 cut(s)	561, 1335, 1352, 1374, 1598, 2016
BfmI	CTRYAG	4 cut(s)	360, 914, 1508, 1674
BfoI	RGCGCY	1 cut(s)	2197
BfuAI	ACCTGC	2 cut(s)	977, 2434
BfuI	GTATCC	1 cut(s)	1447
BglII	AGATCT	1 cut(s)	1727
BisI	GCNGC	9 cut(s)	1508, 1511, 1514, 2022, 2104, 2353, 2356, 2620, 2637
BlsI	GCNGC	9 cut(s)	1509, 1512, 1515, 2023, 2105, 2354, 2357, 2621, 2638
Bme1390I	CCNGG	6 cut(s)	242, 543, 1227, 1317, 2006, 2100
Bme18I	GGWCC	1 cut(s)	1282
BmeRI	GACNNNNNGTC	1 cut(s)	1212
BmeT110I	CYCGRG	2 cut(s)	1648, 2131
BmgBI	CACGTC	1 cut(s)	2380
BmgT120I	GGNCC	7 cut(s)	538, 539, 677, 1282, 1542, 1543, 1556
BmiI	GGNNCC	6 cut(s)	540, 1250, 1284, 1544, 2071, 2563
BmrFI	CCNGG	6 cut(s)	242, 543, 1227, 1317, 2006, 2100
BmrI	ACTGGG	1 cut(s)	723
BmsI	GCATC	5 cut(s)	200, 738, 973, 1268, 1990
BmuI	ACTGGG	1 cut(s)	723
BpmI	CTGGAG	2 cut(s)	948, 1248
Bpu10I	CCTNAGC	1 cut(s)	1026
Bpu14I	TTCGAA	1 cut(s)	1692
BpuEI	CTTGAG	1 cut(s)	823
BsaI	GGTCTC	1 cut(s)	799
BsaJI	CCNNGG	5 cut(s)	541, 893, 1316, 2004, 2098
BsaWI	WCCGGW	1 cut(s)	344
BsaXI	ACNNNNNCTCC	2 cut(s)	2025, 2055
Bse1I	ACTGG	6 cut(s)	85, 169, 718, 978, 1517, 2495
Bse21I	CCTNAGG	1 cut(s)	525
Bse3DI	GCAATG	2 cut(s)	1737, 2176
BseBI	CCWGG	6 cut(s)	242, 543, 1227, 1317, 2006, 2100
BseDI	CCNNGG	5 cut(s)	541, 893, 1316, 2004, 2098
BseGI	GGATG	4 cut(s)	215, 1146, 2005, 2083
BseMI	GCAATG	2 cut(s)	1737, 2176
BseMII	CTCAG	8 cut(s)	234, 383, 479, 639, 1017, 1595, 1740, 1784
BseNI	ACTGG	6 cut(s)	85, 169, 718, 978, 1517, 2495
BseRI	GAGGAG	1 cut(s)	1901
BseSI	GKGCMC	2 cut(s)	542, 1546
BseXI	GCAGC	8 cut(s)	1494, 1522, 2008, 2090, 2364, 2367, 2631, 2648
BsgI	GTGCAG	3 cut(s)	1863, 2089, 2462
Bsh1236I	CGCG	1 cut(s)	2089
BsiHKAI	GWGCWC	1 cut(s)	1871
BsiHKCI	CYCGRG	2 cut(s)	1648, 2131
BsiSI	CCGG	1 cut(s)	345
BslFI	GGGAC	2 cut(s)	188, 1443
BsmAI	GTCTC	8 cut(s)	218, 223, 462, 799, 1600, 1747, 2576, 2585
BsmBI	CGTCTC	1 cut(s)	462
BsmFI	GGGAC	2 cut(s)	188, 1443
BsmI	GAATGC	1 cut(s)	2257
Bso31I	GGTCTC	1 cut(s)	799
BsoBI	CYCGRG	2 cut(s)	1648, 2131
Bsp119I	TTCGAA	1 cut(s)	1692
Bsp120I	GGGCCC	2 cut(s)	538, 1542
Bsp1286I	GDGCHC	5 cut(s)	542, 1546, 1771, 1871, 2427
Bsp143I	GATC	8 cut(s)	291, 349, 575, 1222, 1681, 1727, 2313, 2410
BspACI	CCGC	7 cut(s)	111, 1513, 1795, 2087, 2136, 2246, 2588
BspCNI	CTCAG	8 cut(s)	233, 382, 478, 640, 1018, 1596, 1741, 1783
BspFNI	CGCG	1 cut(s)	2089
BspHI	TCATGA	2 cut(s)	1684, 2208
BspLI	GGNNCC	6 cut(s)	540, 1250, 1284, 1544, 2071, 2563
BspMAI	CTGCAG	1 cut(s)	1512
BspMI	ACCTGC	2 cut(s)	977, 2434
BspPI	GGATC	3 cut(s)	286, 583, 1217
BspT104I	TTCGAA	1 cut(s)	1692
BspTNI	GGTCTC	1 cut(s)	799
BsrBI	CCGCTC	1 cut(s)	2136
BsrDI	GCAATG	2 cut(s)	1737, 2176
BsrI	ACTGG	6 cut(s)	85, 169, 718, 978, 1517, 2495
BssECI	CCNNGG	5 cut(s)	541, 893, 1316, 2004, 2098
BssMI	GATC	8 cut(s)	291, 349, 575, 1222, 1681, 1727, 2313, 2410
BssSI	CACGAG	1 cut(s)	285
BssT1I	CCWWGG	1 cut(s)	893
Bst2BI	CACGAG	1 cut(s)	285
Bst2UI	CCWGG	6 cut(s)	242, 543, 1227, 1317, 2006, 2100
Bst4CI	ACNGT	6 cut(s)	206, 364, 957, 1176, 1204, 2185
Bst6I	CTCTTC	6 cut(s)	727, 860, 1532, 1689, 1718, 2357
BstAPI	GCANNNNNTGC	2 cut(s)	186, 2175
BstBI	TTCGAA	1 cut(s)	1692
BstC8I	GCNNGC	3 cut(s)	2147, 2472, 2488
BstENI	CCTNNNNNAGG	1 cut(s)	852
BstF5I	GGATG	4 cut(s)	215, 1146, 2005, 2083
BstFNI	CGCG	1 cut(s)	2089
BstH2I	RGCGCY	1 cut(s)	2197
BstHHI	GCGC	2 cut(s)	2196, 2399
BstKTI	GATC	8 cut(s)	294, 352, 578, 1225, 1684, 1730, 2316, 2413
BstMAI	GTCTC	8 cut(s)	218, 223, 462, 799, 1600, 1747, 2576, 2585
BstMBI	GATC	8 cut(s)	291, 349, 575, 1222, 1681, 1727, 2313, 2410
BstNI	CCWGG	6 cut(s)	242, 543, 1227, 1317, 2006, 2100
BstNSI	RCATGY	4 cut(s)	1023, 1839, 2163, 2169
BstSCI	CCNGG	6 cut(s)	240, 541, 1225, 1315, 2004, 2098
BstSFI	CTRYAG	4 cut(s)	360, 914, 1508, 1674
BstSLI	GKGCMC	2 cut(s)	542, 1546
BstUI	CGCG	1 cut(s)	2089
BstV1I	GCAGC	8 cut(s)	1494, 1522, 2008, 2090, 2364, 2367, 2631, 2648
BstX2I	RGATCY	2 cut(s)	1222, 1727
BstYI	RGATCY	2 cut(s)	1222, 1727
Bsu36I	CCTNAGG	1 cut(s)	525
BsuI	GTATCC	1 cut(s)	1447
BtgZI	GCGATG	1 cut(s)	2078
BtrI	CACGTC	1 cut(s)	2380
BtsCI	GGATG	4 cut(s)	215, 1146, 2005, 2083
BtsIMutI	CAGTG	5 cut(s)	202, 953, 985, 1524, 1778
BveI	ACCTGC	2 cut(s)	977, 2434
Cac8I	GCNNGC	3 cut(s)	2147, 2472, 2488
CciI	TCATGA	2 cut(s)	1684, 2208
CfoI	GCGC	2 cut(s)	2196, 2399
Cfr13I	GGNCC	7 cut(s)	538, 539, 677, 1282, 1542, 1543, 1556
CseI	GACGC	2 cut(s)	2078, 2486
DpnI	GATC	8 cut(s)	293, 351, 577, 1224, 1683, 1729, 2315, 2412
DpnII	GATC	8 cut(s)	291, 349, 575, 1222, 1681, 1727, 2313, 2410
DraI	TTTAAA	1 cut(s)	1938
DraIII	CACNNNGTG	1 cut(s)	829
DrdI	GACNNNNNNGTC	1 cut(s)	2088
DriI	GACNNNNNGTC	1 cut(s)	1212
DseDI	GACNNNNNNGTC	1 cut(s)	2088
EaeI	YGGCCR	4 cut(s)	39, 890, 1121, 1514
Eam1104I	CTCTTC	6 cut(s)	727, 860, 1532, 1689, 1718, 2357
Eam1105I	GACNNNNNGTC	1 cut(s)	1212
EarI	CTCTTC	6 cut(s)	727, 860, 1532, 1689, 1718, 2357
EciI	GGCGGA	1 cut(s)	1810
Eco130I	CCWWGG	1 cut(s)	893
Eco147I	AGGCCT	1 cut(s)	1062
Eco24I	GRGCYC	4 cut(s)	542, 1546, 1771, 2427
Eco31I	GGTCTC	1 cut(s)	799
Eco47I	GGWCC	1 cut(s)	1282
Eco57I	CTGAAG	2 cut(s)	873, 1467
Eco81I	CCTNAGG	1 cut(s)	525
Eco88I	CYCGRG	2 cut(s)	1648, 2131
EcoNI	CCTNNNNNAGG	1 cut(s)	852
EcoO109I	RGGNCCY	2 cut(s)	1282, 1542
EcoRI	GAATTC	3 cut(s)	127, 937, 1688
EcoRII	CCWGG	6 cut(s)	240, 541, 1225, 1315, 2004, 2098
EcoT14I	CCWWGG	1 cut(s)	893
EcoT22I	ATGCAT	1 cut(s)	215
EcoT38I	GRGCYC	4 cut(s)	542, 1546, 1771, 2427
ErhI	CCWWGG	1 cut(s)	893
Esp3I	CGTCTC	1 cut(s)	462
FalI	AAGNNNNNCTT	4 cut(s)	832, 864, 2607, 2639
FaqI	GGGAC	2 cut(s)	188, 1443
FauI	CCCGC	1 cut(s)	2129
FbaI	TGATCA	1 cut(s)	349
FblI	GTMKAC	1 cut(s)	1809
Fnu4HI	GCNGC	9 cut(s)	1508, 1511, 1514, 2022, 2104, 2353, 2356, 2620, 2637
FokI	GGATG	4 cut(s)	222, 1153, 2012, 2070
FriOI	GRGCYC	4 cut(s)	542, 1546, 1771, 2427
Fsp4HI	GCNGC	9 cut(s)	1508, 1511, 1514, 2022, 2104, 2353, 2356, 2620, 2637
FspBI	CTAG	6 cut(s)	561, 1335, 1352, 1374, 1598, 2016
GlaI	GCGC	2 cut(s)	2195, 2398
GluI	GCNGC	9 cut(s)	1508, 1511, 1514, 2022, 2104, 2353, 2356, 2620, 2637
GsuI	CTGGAG	2 cut(s)	948, 1248
HaeII	RGCGCY	1 cut(s)	2197
HapII	CCGG	1 cut(s)	345
HgaI	GACGC	2 cut(s)	2078, 2486
HhaI	GCGC	2 cut(s)	2196, 2399
Hin6I	GCGC	2 cut(s)	2194, 2397
HinP1I	GCGC	2 cut(s)	2194, 2397
HincII	GTYRAC	1 cut(s)	1810
HindII	GTYRAC	1 cut(s)	1810
HpaII	CCGG	1 cut(s)	345
HphI	GGTGA	6 cut(s)	359, 485, 815, 1208, 1903, 2066
Hpy166II	GTNNAC	7 cut(s)	136, 823, 1643, 1810, 1833, 1960, 2500
Hpy188III	TCNNGA	8 cut(s)	295, 961, 1079, 1263, 1428, 1598, 1685, 2209
Hpy8I	GTNNAC	7 cut(s)	136, 823, 1643, 1810, 1833, 1960, 2500
Hpy99I	CGWCG	1 cut(s)	1814
HpyCH4III	ACNGT	6 cut(s)	206, 364, 957, 1176, 1204, 2185
HpyCH4IV	ACGT	6 cut(s)	146, 1103, 1812, 2239, 2379, 2537
HpySE526I	ACGT	6 cut(s)	146, 1103, 1812, 2239, 2379, 2537
HspAI	GCGC	2 cut(s)	2194, 2397
Ksp22I	TGATCA	1 cut(s)	349
Kzo9I	GATC	8 cut(s)	291, 349, 575, 1222, 1681, 1727, 2313, 2410
LmnI	GCTCC	2 cut(s)	377, 2069
Lsp1109I	GCAGC	8 cut(s)	1494, 1522, 2008, 2090, 2364, 2367, 2631, 2648
LweI	GCATC	5 cut(s)	200, 738, 973, 1268, 1990
MaeI	CTAG	6 cut(s)	561, 1335, 1352, 1374, 1598, 2016
MaeII	ACGT	6 cut(s)	146, 1103, 1812, 2239, 2379, 2537
MaeIII	GTNAC	8 cut(s)	514, 873, 1159, 1204, 1354, 1484, 1669, 2061
MalI	GATC	8 cut(s)	293, 351, 577, 1224, 1683, 1729, 2315, 2412
MbiI	CCGCTC	1 cut(s)	2136
MboI	GATC	8 cut(s)	291, 349, 575, 1222, 1681, 1727, 2313, 2410
MfeI	CAATTG	1 cut(s)	416
MflI	RGATCY	2 cut(s)	1222, 1727
MhlI	GDGCHC	5 cut(s)	542, 1546, 1771, 1871, 2427
MlsI	TGGCCA	2 cut(s)	892, 1123
MluNI	TGGCCA	2 cut(s)	892, 1123
MlyI	GAGTC	2 cut(s)	877, 2595
MmeI	TCCRAC	4 cut(s)	149, 471, 1196, 2622
Mox20I	TGGCCA	2 cut(s)	892, 1123
Mph1103I	ATGCAT	1 cut(s)	215
MroXI	GAANNNNTTC	2 cut(s)	1062, 1593
MscI	TGGCCA	2 cut(s)	892, 1123
MseI	TTAA	6 cut(s)	311, 383, 581, 1490, 1788, 1937
MslI	CAYNNNNRTG	4 cut(s)	831, 1470, 2164, 2436
Msp20I	TGGCCA	2 cut(s)	892, 1123
MspA1I	CMGCKG	1 cut(s)	1513
MspI	CCGG	1 cut(s)	345
MspR9I	CCNGG	6 cut(s)	242, 543, 1227, 1317, 2006, 2100
MunI	CAATTG	1 cut(s)	416
Mva1269I	GAATGC	1 cut(s)	2257
MvaI	CCWGG	6 cut(s)	242, 543, 1227, 1317, 2006, 2100
MvnI	CGCG	1 cut(s)	2089
NdeII	GATC	8 cut(s)	291, 349, 575, 1222, 1681, 1727, 2313, 2410
NlaIV	GGNNCC	6 cut(s)	540, 1250, 1284, 1544, 2071, 2563
NmuCI	GTSAC	3 cut(s)	1204, 1484, 2061
NsiI	ATGCAT	1 cut(s)	215
NspI	RCATGY	4 cut(s)	1023, 1839, 2163, 2169
NspV	TTCGAA	1 cut(s)	1692
PagI	TCATGA	2 cut(s)	1684, 2208
PceI	AGGCCT	1 cut(s)	1062
PcsI	WCGNNNNNNNCGW	1 cut(s)	997
PctI	GAATGC	1 cut(s)	2257
PdmI	GAANNNNTTC	2 cut(s)	1062, 1593
PfeI	GAWTC	8 cut(s)	22, 443, 1075, 1348, 1745, 1950, 2285, 2329
PflMI	CCANNNNNTGG	4 cut(s)	430, 572, 602, 2552
PfoI	TCCNGGA	1 cut(s)	1225
PkrI	GCNGC	9 cut(s)	1509, 1512, 1515, 2023, 2105, 2354, 2357, 2621, 2638
PleI	GAGTC	2 cut(s)	876, 2595
PpsI	GAGTC	2 cut(s)	876, 2595
PpuMI	RGGWCCY	1 cut(s)	1282
Psp5II	RGGWCCY	1 cut(s)	1282
Psp6I	CCWGG	6 cut(s)	240, 541, 1225, 1315, 2004, 2098
PspGI	CCWGG	6 cut(s)	240, 541, 1225, 1315, 2004, 2098
PspN4I	GGNNCC	6 cut(s)	540, 1250, 1284, 1544, 2071, 2563
PspOMI	GGGCCC	2 cut(s)	538, 1542
PspPI	GGNCC	7 cut(s)	538, 539, 677, 1282, 1542, 1543, 1556
PspPPI	RGGWCCY	1 cut(s)	1282
PstI	CTGCAG	1 cut(s)	1512
PsuI	RGATCY	2 cut(s)	1222, 1727
RseI	CAYNNNNRTG	4 cut(s)	831, 1470, 2164, 2436
SalI	GTCGAC	1 cut(s)	1808
SaqAI	TTAA	6 cut(s)	311, 383, 581, 1490, 1788, 1937
SatI	GCNGC	9 cut(s)	1508, 1511, 1514, 2022, 2104, 2353, 2356, 2620, 2637
Sau3AI	GATC	8 cut(s)	291, 349, 575, 1222, 1681, 1727, 2313, 2410
Sau96I	GGNCC	7 cut(s)	538, 539, 677, 1282, 1542, 1543, 1556
SchI	GAGTC	2 cut(s)	877, 2595
ScrFI	CCNGG	6 cut(s)	242, 543, 1227, 1317, 2006, 2100
SduI	GDGCHC	5 cut(s)	542, 1546, 1771, 1871, 2427
SfaNI	GCATC	5 cut(s)	200, 738, 973, 1268, 1990
SfcI	CTRYAG	4 cut(s)	360, 914, 1508, 1674
SfuI	TTCGAA	1 cut(s)	1692
SinI	GGWCC	1 cut(s)	1282
SmiMI	CAYNNNNRTG	4 cut(s)	831, 1470, 2164, 2436
SmlI	CTYRAG	1 cut(s)	802
SmoI	CTYRAG	1 cut(s)	802
SseBI	AGGCCT	1 cut(s)	1062
SsiI	CCGC	7 cut(s)	111, 1513, 1795, 2087, 2136, 2246, 2588
SspI	AATATT	1 cut(s)	1552
SspMI	CTAG	6 cut(s)	561, 1335, 1352, 1374, 1598, 2016
StuI	AGGCCT	1 cut(s)	1062
StyD4I	CCNGG	6 cut(s)	240, 541, 1225, 1315, 2004, 2098
StyI	CCWWGG	1 cut(s)	893
TaaI	ACNGT	6 cut(s)	206, 364, 957, 1176, 1204, 2185
TaiI	ACGT	6 cut(s)	149, 1106, 1815, 2242, 2382, 2540
TaqI	TCGA	5 cut(s)	960, 991, 1692, 1809, 2316
TauI	GCSGC	1 cut(s)	1516
TfiI	GAWTC	8 cut(s)	22, 443, 1075, 1348, 1745, 1950, 2285, 2329
Tru1I	TTAA	6 cut(s)	311, 383, 581, 1490, 1788, 1937
Tru9I	TTAA	6 cut(s)	311, 383, 581, 1490, 1788, 1937
TscAI	CASTG	5 cut(s)	209, 960, 985, 1524, 1778
TseFI	GTSAC	3 cut(s)	1204, 1484, 2061
TseI	GCWGC	8 cut(s)	1507, 1510, 2021, 2103, 2352, 2355, 2619, 2636
Tsp45I	GTSAC	3 cut(s)	1204, 1484, 2061
TspGWI	ACGGA	1 cut(s)	2620
TspRI	CASTG	5 cut(s)	209, 960, 985, 1524, 1778
Van91I	CCANNNNNTGG	4 cut(s)	430, 572, 602, 2552
VpaK11BI	GGWCC	1 cut(s)	1282
XagI	CCTNNNNNAGG	1 cut(s)	852
XapI	RAATTY	8 cut(s)	127, 694, 720, 777, 937, 1525, 1688, 1846
XbaI	TCTAGA	1 cut(s)	1597
XceI	RCATGY	4 cut(s)	1023, 1839, 2163, 2169
XmiI	GTMKAC	1 cut(s)	1809
XmnI	GAANNNNTTC	2 cut(s)	1062, 1593
XspI	CTAG	6 cut(s)	561, 1335, 1352, 1374, 1598, 2016
Zsp2I	ATGCAT	1 cut(s)	215

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.