Rorug05G0422100

CW-type Zinc Finger

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0006399
Physical Location & Seq
Forward (+)
58679802 .. 58681272
1471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0422100.1

Sequence Viewer

Length: 1320 bp
ATGGCTAAAGTTAGAGGGTCATATATTTTGTTGGCTCTCTTTGTAGTGCTCATTTCGATGGAAGCCTCTGTTGTTTTTGGTCAAGCCAACGGCAATGGAAATGCGAATGGAAACGGCAACACTGGCAGTGGCAACGGAAATGCCAACGGCAATGGCAACACCGGCAGTGGCAACGGAAATGGGAACGGCAATGGCAACACTGGCAGTGGCAACGGCAATGGGAATGGGAACGGCAACACTGGCAGTGGCAATGGTAACGGGAATGGGAACGGCAATACTGGCAGTGGCAACGGAAACGGGAATGGAAACGGCAACGGGAATGGCAATGGCAATGGAAACGGAAACGGGAATGGGAATGGAAATCAAGTTAATGCTGATGTTACAAACTACCAGGTATTGTCACCACTAGCAACCGGACAAGAGAGATGCATGTGCAAAGCTCAGGGCACCTGTTTCTACAAGACCCTTGTCTGTCCACCGGAGTGCCCTCAGAGAAAGCCTAAGCAGAACAAGAAACACAAGGGCTGCTTCGTCAACTGCGGCAGCAAGTGTGAAGTCACCTGCAAGTTTAGAAGAGCCAACTGTAATGGGTATGGTTCTCTCTGCTATGATCCTCGATTTGTTGGTGGAGATGGTGTTATGTTCTACTTCCATGGAGCCAAGGGAGGAAACTTTGCCATTGTTTCAGACGATAACCTCCAGATCAATGCACACTTCATTGGGACTCGACCACTAGGAAGGACTCGCGACTTCACATGGGTCCAAGCCCTCTCAATCATGTTCGACAATCACAACCTTGTCATTGCAGCAAAGAGGGTCTCAAAGTGGGATGACAAAGTTGATGCTCTCATCATAAGGTGGGATGGTGAGTCGGTTAGCATTCCTTCGGATGGAGAAGCCGAATGGAGGACTAATGGTAATCAAGAACGACAGGTAATCGTGGAAAGAACCGATGATACTAACAACGTAAGAGTTACAGTTGCAGGGCTACTGGAAATGGACATAAAGATTAGGCCTATTGGAGAAGAAGAAAACAAGGTTCATAAGTATCAAATACCAGCTGATGATACTTTTGCTCACTTGGAAACACAGTTCAGATTTTCGAACTTATCTGATCTTGTCGAAGGAGTTTTGGGCAAGACTTATAGGCCAGGTTATGTTAGTCCAGTCAAGGTTGGAGTTCCAATGCCAATGATGGGTGGGGAGGACAAGTACAGAACTCCTTCACTATTCTCACCTCTCTGCAAGGTTTGCAGGTTCCAAACTACTAGTCAATCTTCAGGGTTTGAAATTACAAGTGGAGGAGTGGCATCATATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
B3 zf-CW

Protein Analysis

439

Amino Acids

46.46

Weight (kDa)

8.49

Isoelectric Point (pI)

4.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Root_cap PF06830 364 - 420 1.8e-29 Root cap
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 569
Acc36I ACCTGC 2 cut(s) 569, 1245
AccB1I GGYRCC 1 cut(s) 446
AccII CGCG 1 cut(s) 747
AciI CCGC 1 cut(s) 540
AclWI GGATC 1 cut(s) 605
AcuI CTGAAG 1 cut(s) 1263
AfaI GTAC 1 cut(s) 1214
AfiI CCNNNNNNNGG 3 cut(s) 890, 906, 1196
AgsI TTSAA 1 cut(s) 1289
AhlI ACTAGT 1 cut(s) 1268
AjnI CCWGG 2 cut(s) 390, 1150
AleI CACNNNNGTG 1 cut(s) 481
AloI GAACNNNNNNTCC 2 cut(s) 1076, 1108
AluBI AGCT 2 cut(s) 440, 1061
AluI AGCT 2 cut(s) 440, 1061
Alw21I GWGCWC 1 cut(s) 51
Alw26I GTCTC 1 cut(s) 823
AlwI GGATC 1 cut(s) 605
AoxI GGCC 2 cut(s) 1013, 1148
ApeKI GCWGC 3 cut(s) 525, 543, 806
ArsI GACNNNNNNTTYG 2 cut(s) 1255, 1287
Asp700I GAANNNNTTC 1 cut(s) 1222
AspS9I GGNCC 1 cut(s) 760
AsuHPI GGTGA 4 cut(s) 393, 550, 878, 1227
AsuII TTCGAA 1 cut(s) 1103
AvaII GGWCC 1 cut(s) 760
BaeGI GKGCMC 2 cut(s) 449, 488
BanI GGYRCC 1 cut(s) 446
Bbv12I GWGCWC 1 cut(s) 51
BbvI GCAGC 3 cut(s) 512, 555, 818
BccI CCATC 5 cut(s) 52, 626, 857, 884, 1189
BceAI ACGGC 8 cut(s) 106, 130, 163, 202, 229, 247, 286, 325
BciT130I CCWGG 2 cut(s) 392, 1152
BcoDI GTCTC 1 cut(s) 823
BcuI ACTAGT 1 cut(s) 1268
BfaI CTAG 3 cut(s) 407, 734, 1269
BfuAI ACCTGC 2 cut(s) 569, 1245
BisI GCNGC 4 cut(s) 526, 541, 544, 807
BlsI GCNGC 4 cut(s) 527, 542, 545, 808
Bme1390I CCNGG 2 cut(s) 392, 1152
Bme18I GGWCC 1 cut(s) 760
BmgT120I GGNCC 1 cut(s) 760
BmiI GGNNCC 4 cut(s) 448, 658, 761, 1259
BmrFI CCNGG 2 cut(s) 392, 1152
BmsI GCATC 2 cut(s) 416, 832
BoxI GACNNNNGTC 1 cut(s) 467
BpmI CTGGAG 1 cut(s) 683
Bpu10I CCTNAGC 2 cut(s) 441, 501
Bpu14I TTCGAA 1 cut(s) 1103
BsaI GGTCTC 1 cut(s) 823
BsaJI CCNNGG 2 cut(s) 652, 660
BsaWI WCCGGW 2 cut(s) 413, 478
BsaXI ACNNNNNCTCC 4 cut(s) 621, 651, 1196, 1226
Bsc4I CCNNNNNNNGG 3 cut(s) 890, 906, 1196
Bse118I RCCGGY 1 cut(s) 161
Bse1I ACTGG 6 cut(s) 127, 205, 244, 283, 996, 1166
Bse3DI GCAATG 8 cut(s) 100, 157, 196, 223, 256, 331, 337, 801
BseBI CCWGG 2 cut(s) 392, 1152
BseDI CCNNGG 2 cut(s) 652, 660
BseGI GGATG 3 cut(s) 835, 868, 895
BseLI CCNNNNNNNGG 3 cut(s) 890, 906, 1196
BseMI GCAATG 8 cut(s) 100, 157, 196, 223, 256, 331, 337, 801
BseMII CTCAG 2 cut(s) 455, 503
BseNI ACTGG 6 cut(s) 127, 205, 244, 283, 996, 1166
BseRI GAGGAG 1 cut(s) 1317
BseSI GKGCMC 2 cut(s) 449, 488
BseXI GCAGC 3 cut(s) 512, 555, 818
Bsh1236I CGCG 1 cut(s) 747
BshFI GGCC 2 cut(s) 1015, 1150
BshNI GGYRCC 1 cut(s) 446
BsiHKAI GWGCWC 1 cut(s) 51
BsiSI CCGG 3 cut(s) 162, 414, 479
BslFI GGGAC 1 cut(s) 736
BslI CCNNNNNNNGG 3 cut(s) 890, 906, 1196
BsmAI GTCTC 1 cut(s) 823
BsmFI GGGAC 1 cut(s) 736
BsmI GAATGC 1 cut(s) 879
BsnI GGCC 2 cut(s) 1015, 1150
Bso31I GGTCTC 1 cut(s) 823
Bsp119I TTCGAA 1 cut(s) 1103
Bsp1286I GDGCHC 3 cut(s) 51, 449, 488
Bsp143I GATC 3 cut(s) 610, 702, 1114
Bsp19I CCATGG 1 cut(s) 652
Bsp68I TCGCGA 1 cut(s) 747
BspACI CCGC 1 cut(s) 540
BspANI GGCC 2 cut(s) 1015, 1150
BspCNI CTCAG 2 cut(s) 454, 502
BspFNI CGCG 1 cut(s) 747
BspLI GGNNCC 4 cut(s) 448, 658, 761, 1259
BspMI ACCTGC 2 cut(s) 569, 1245
BspPI GGATC 1 cut(s) 605
BspQI GCTCTTC 1 cut(s) 568
BspT104I TTCGAA 1 cut(s) 1103
BspT107I GGYRCC 1 cut(s) 446
BspTNI GGTCTC 1 cut(s) 823
BsrDI GCAATG 8 cut(s) 100, 157, 196, 223, 256, 331, 337, 801
BsrFI RCCGGY 1 cut(s) 161
BsrI ACTGG 6 cut(s) 127, 205, 244, 283, 996, 1166
BssAI RCCGGY 1 cut(s) 161
BssECI CCNNGG 2 cut(s) 652, 660
BssMI GATC 3 cut(s) 610, 702, 1114
BssT1I CCWWGG 2 cut(s) 652, 660
Bst2UI CCWGG 2 cut(s) 392, 1152
Bst4CI ACNGT 3 cut(s) 584, 979, 1092
Bst6I CTCTTC 1 cut(s) 568
BstAPI GCANNNNNTGC 1 cut(s) 1251
BstBI TTCGAA 1 cut(s) 1103
BstDEI CTNAG 3 cut(s) 441, 489, 501
BstDSI CCRYGG 1 cut(s) 652
BstF5I GGATG 3 cut(s) 835, 868, 895
BstFNI CGCG 1 cut(s) 747
BstKTI GATC 3 cut(s) 613, 705, 1117
BstMAI GTCTC 1 cut(s) 823
BstMBI GATC 3 cut(s) 610, 702, 1114
BstMWI GCNNNNNNNGC 6 cut(s) 123, 162, 201, 240, 279, 1251
BstNI CCWGG 2 cut(s) 392, 1152
BstNSI RCATGY 1 cut(s) 433
BstPAI GACNNNNGTC 1 cut(s) 467
BstSCI CCNGG 2 cut(s) 390, 1150
BstSLI GKGCMC 2 cut(s) 449, 488
BstUI CGCG 1 cut(s) 747
BstV1I GCAGC 3 cut(s) 512, 555, 818
BsuRI GGCC 2 cut(s) 1015, 1150
BtgI CCRYGG 1 cut(s) 652
BtsCI GGATG 3 cut(s) 835, 868, 895
BtsI GCAGTG 5 cut(s) 133, 172, 211, 250, 289
BtsIMutI CAGTG 8 cut(s) 120, 133, 172, 198, 211, 237, 250, 289
BtuMI TCGCGA 1 cut(s) 747
BveI ACCTGC 2 cut(s) 569, 1245
Cfr10I RCCGGY 1 cut(s) 161
Cfr13I GGNCC 1 cut(s) 760
CsiI ACCWGGT 1 cut(s) 390
Csp6I GTAC 1 cut(s) 1213
CviAII CATG 4 cut(s) 430, 653, 756, 778
CviQI GTAC 1 cut(s) 1213
DdeI CTNAG 3 cut(s) 441, 489, 501
DpnI GATC 3 cut(s) 612, 704, 1116
DpnII GATC 3 cut(s) 610, 702, 1114
Eam1104I CTCTTC 1 cut(s) 568
EarI CTCTTC 1 cut(s) 568
Eco130I CCWWGG 2 cut(s) 652, 660
Eco147I AGGCCT 1 cut(s) 1015
Eco31I GGTCTC 1 cut(s) 823
Eco47I GGWCC 1 cut(s) 760
Eco57I CTGAAG 1 cut(s) 1263
EcoRII CCWGG 2 cut(s) 390, 1150
EcoT14I CCWWGG 2 cut(s) 652, 660
EcoT22I ATGCAT 1 cut(s) 431
ErhI CCWWGG 2 cut(s) 652, 660
FaeI CATG 4 cut(s) 433, 656, 759, 781
FalI AAGNNNNNCTT 2 cut(s) 512, 544
FaqI GGGAC 1 cut(s) 736
FatI CATG 4 cut(s) 429, 652, 755, 777
Fnu4HI GCNGC 4 cut(s) 526, 541, 544, 807
FokI GGATG 3 cut(s) 842, 875, 902
Fsp4HI GCNGC 4 cut(s) 526, 541, 544, 807
FspBI CTAG 3 cut(s) 407, 734, 1269
GluI GCNGC 4 cut(s) 526, 541, 544, 807
GsuI CTGGAG 1 cut(s) 683
HaeIII GGCC 2 cut(s) 1015, 1150
HapII CCGG 3 cut(s) 162, 414, 479
Hin1II CATG 4 cut(s) 433, 656, 759, 781
HincII GTYRAC 1 cut(s) 535
HindII GTYRAC 1 cut(s) 535
HinfI GANTC 3 cut(s) 724, 742, 869
HpaII CCGG 3 cut(s) 162, 414, 479
HphI GGTGA 4 cut(s) 393, 550, 878, 1227
Hpy166II GTNNAC 2 cut(s) 476, 535
Hpy188I TCNGA 5 cut(s) 492, 688, 889, 1097, 1114
Hpy188III TCNNGA 3 cut(s) 700, 746, 923
Hpy8I GTNNAC 2 cut(s) 476, 535
HpyAV CCTTC 4 cut(s) 732, 894, 1118, 1233
HpyCH4III ACNGT 3 cut(s) 584, 979, 1092
HpyCH4IV ACGT 1 cut(s) 966
HpyCH4V TGCA 8 cut(s) 429, 435, 564, 710, 806, 983, 1245, 1254
HpyF10VI GCNNNNNNNGC 6 cut(s) 123, 162, 201, 240, 279, 1251
HpyF3I CTNAG 3 cut(s) 441, 489, 501
HpySE526I ACGT 1 cut(s) 966
Hsp92II CATG 4 cut(s) 433, 656, 759, 781
Kzo9I GATC 3 cut(s) 610, 702, 1114
LguI GCTCTTC 1 cut(s) 568
LmnI GCTCC 1 cut(s) 656
Lsp1109I GCAGC 3 cut(s) 512, 555, 818
LweI GCATC 2 cut(s) 416, 832
MabI ACCWGGT 1 cut(s) 390
MaeI CTAG 3 cut(s) 407, 734, 1269
MaeII ACGT 1 cut(s) 966
MaeIII GTNAC 5 cut(s) 254, 379, 399, 556, 973
MalI GATC 3 cut(s) 612, 704, 1116
MboI GATC 3 cut(s) 610, 702, 1114
MboII GAAGA 4 cut(s) 585, 1037, 1040, 1269
MhlI GDGCHC 3 cut(s) 51, 449, 488
MluCI AATT 1 cut(s) 1290
MlyI GAGTC 3 cut(s) 718, 736, 878
MmeI TCCRAC 1 cut(s) 1156
Mph1103I ATGCAT 1 cut(s) 431
MroXI GAANNNNTTC 1 cut(s) 1222
MseI TTAA 1 cut(s) 369
MslI CAYNNNNRTG 2 cut(s) 56, 481
MspA1I CMGCKG 1 cut(s) 1061
MspI CCGG 3 cut(s) 162, 414, 479
MspR9I CCNGG 2 cut(s) 392, 1152
Mva1269I GAATGC 1 cut(s) 879
MvaI CCWGG 2 cut(s) 392, 1152
MvnI CGCG 1 cut(s) 747
MwoI GCNNNNNNNGC 6 cut(s) 123, 162, 201, 240, 279, 1251
NcoI CCATGG 1 cut(s) 652
NdeII GATC 3 cut(s) 610, 702, 1114
NlaIII CATG 4 cut(s) 433, 656, 759, 781
NlaIV GGNNCC 4 cut(s) 448, 658, 761, 1259
NmuCI GTSAC 2 cut(s) 399, 556
NruI TCGCGA 1 cut(s) 747
NsiI ATGCAT 1 cut(s) 431
NspI RCATGY 1 cut(s) 433
NspV TTCGAA 1 cut(s) 1103
OliI CACNNNNGTG 1 cut(s) 481
PaqCI CACCTGC 1 cut(s) 569
PceI AGGCCT 1 cut(s) 1015
PciSI GCTCTTC 1 cut(s) 568
PctI GAATGC 1 cut(s) 879
PdmI GAANNNNTTC 1 cut(s) 1222
PkrI GCNGC 4 cut(s) 527, 542, 545, 808
PleI GAGTC 3 cut(s) 718, 736, 877
PpsI GAGTC 3 cut(s) 718, 736, 877
PshAI GACNNNNGTC 1 cut(s) 467
Psp6I CCWGG 2 cut(s) 390, 1150
PspGI CCWGG 2 cut(s) 390, 1150
PspN4I GGNNCC 4 cut(s) 448, 658, 761, 1259
PspPI GGNCC 1 cut(s) 760
PsrI GAACNNNNNNTAC 2 cut(s) 940, 972
PvuII CAGCTG 1 cut(s) 1061
RruI TCGCGA 1 cut(s) 747
RsaI GTAC 1 cut(s) 1214
RsaNI GTAC 1 cut(s) 1213
RseI CAYNNNNRTG 2 cut(s) 56, 481
SapI GCTCTTC 1 cut(s) 568
SaqAI TTAA 1 cut(s) 369
SatI GCNGC 4 cut(s) 526, 541, 544, 807
Sau3AI GATC 3 cut(s) 610, 702, 1114
Sau96I GGNCC 1 cut(s) 760
SchI GAGTC 3 cut(s) 718, 736, 878
ScrFI CCNGG 2 cut(s) 392, 1152
SduI GDGCHC 3 cut(s) 51, 449, 488
SexAI ACCWGGT 1 cut(s) 390
SfaNI GCATC 2 cut(s) 416, 832
SfuI TTCGAA 1 cut(s) 1103
SinI GGWCC 1 cut(s) 760
SmiMI CAYNNNNRTG 2 cut(s) 56, 481
SpeI ACTAGT 1 cut(s) 1268
Sse9I AATT 1 cut(s) 1290
SseBI AGGCCT 1 cut(s) 1015
SsiI CCGC 1 cut(s) 540
SspMI CTAG 3 cut(s) 407, 734, 1269
StuI AGGCCT 1 cut(s) 1015
StyD4I CCNGG 2 cut(s) 390, 1150
StyI CCWWGG 2 cut(s) 652, 660
TaaI ACNGT 3 cut(s) 584, 979, 1092
TaiI ACGT 1 cut(s) 969
TaqI TCGA 6 cut(s) 56, 616, 727, 783, 1103, 1122
TasI AATT 1 cut(s) 1290
TatI WGTACW 1 cut(s) 1212
TauI GCSGC 1 cut(s) 543
Tru1I TTAA 1 cut(s) 369
Tru9I TTAA 1 cut(s) 369
TscAI CASTG 8 cut(s) 127, 133, 172, 205, 211, 244, 250, 289
TseFI GTSAC 2 cut(s) 399, 556
TseI GCWGC 3 cut(s) 525, 543, 806
Tsp45I GTSAC 2 cut(s) 399, 556
TspDTI ATGAA 2 cut(s) 706, 1031
TspGWI ACGGA 4 cut(s) 150, 189, 306, 354
TspRI CASTG 8 cut(s) 127, 133, 172, 205, 211, 244, 250, 289
VpaK11BI GGWCC 1 cut(s) 760
XceI RCATGY 1 cut(s) 433
XmnI GAANNNNTTC 1 cut(s) 1222
XspI CTAG 3 cut(s) 407, 734, 1269
Zsp2I ATGCAT 1 cut(s) 431
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.