Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0010972

Physical Location & Seq

Genomic Coordinates Forward (+)

4525548 .. 4529182

Gene Structure 3635 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1056600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1758 bp

ATGATGGGCTCTGAGAACCGGGGCTCCGACGAAGCCCACCTCCACGCGCCGAAAGAAGAAATGGAAAATCTCGTCCTCGACGATCAATCGAACGGCAATAAATCGTACTCGAATTATCGCAGCGCCATGTCGTCGCTCTCCGATACGACACACCCTCTCTCGTCGTCGCCGTCCTCCTCCCAGATCGTATCCGAAGTCCCCGCCACCACCGCCAATACAACGCCACAGGACGACGCCGATCCCCTGTTCGTTCCCGCACCGTACCGCGACCTCCGAAACCCTAGCTCGGCCCCGGACAACACCACTTACATCGACCCGCCTTCTTACGCCGACGTGATCTACAGCCCCTACGATGCCGAAACCGCCGCCGAAATCCACGGCATCGAGAGTCCTGGTAAGAGCTCCGACAACTCGAGCTCGTACTCTCTCTCGCGATCTCCGTCTTCCACATCCGAGTACATCAAAATCACGGTCTCGAATCCGCAGAAGGAGCCGGAAAGCGGGAATTCGATCGTTCCGGGAGCTAACAATTACGTCACGTACCTGATCACGACTCGGACGAACATTCCGGATTTCGGCGGATCGGAATTCGGAGTCCGGCGGCGGTTTCGGGACGTGGTAACGTTATCAGACCGGTTGGCGGAGTCGTACAGAGGGTTCTTTATACCGCCGAGGCCAGACAAGAGCGTGGTGGAGAGTCAGGTGATGCAGAAACAGGAGTTTGTGGAGCAGAGAAGAGTGGCATTGGAGAAGTACTTGAGGCGATTGGCTTCGCACCCGGTGATCAAGAGAAGCGACGAATTGAAGGTGTTTTTGCAGGTGCAGGGGAAGCTACCCCTGGCATTGACCACTGATGTGGCGTCTAGGATGCTTGATGGGGCCGTGAAGCTTCCGAATCAGTTGTTTTCGGAGAGTGCTCCGCCACTGGCGCTTCACGAGGTGGTTCAGCCTGCGAAAGGGGGCAGGGATTTGTTGAGGTTGTTCAAGGAGTTGAAGCAGTCTGTGGCTAATGATTGGGGAGCTTCGAGGCCGCTGGTGGGGGAGGAGGATAAGGAGTTTTTAGAAAAGAAAGAGAAGATGAATGAGCTGGAGCAACATCTCATTCATGCATCTCAGCAGGCCGAATCACTCGTTAAAGCTCAACAGGACATGGGGGAGACGATGGGAGAGTTAGGACTAGCGTTTATTAAGCTGACCAAGTTTGAGAACGAGGAGGCCTTGTTCAACTCTCAGAGAGTGCGGGCTGCCGACATGAAGAATGTGGCAACTGCTGCAGTCAAAGCCAGCAGATTCTATCGGGAGTTAAATGCTCAAACCGTCAAGCATTTCGATACATTTCATGAATATCTTGGGCTAATGTTAGCTGTCAATGGTGCATTCTCGGATAGGTCAAGTGCTCTATTGACAGTGCAGACTCTTCTATCAGATCTGGGTTCGCTGCAATCACGGGCTGAAAAACTTGAAGTTCAATCCAATAAAATTTTTGGTGGTGACAAATCAAGGAATCGAAAACTAGAGGAGTTAAATGAAACCATAAGAGCCACTGAGGATGCTAAAAATGTAGCAATCAGAGAATATGAGCGGATCAAGGAAAATAACAGGAGTGAACTTGAAAGACTTGACAATGAGAGGCATGCTGACTTCTTGAACATGTTGAAGGGATTTGTGCATAATCAGGTGGGATACGCTGAAAAGATAGCAAATGTGTGGTCGACTGTTGCAGAGGAGACCCGTTCGTATGCAAAAGAGAGCCCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000139 GO:0003674 GO:0005488 GO:0005515 GO:0005543 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005768 GO:0005770 GO:0005771 GO:0005794 GO:0005802 GO:0005829 GO:0006807 GO:0006810 GO:0006897 GO:0006996 GO:0007034 GO:0007275 GO:0008150 GO:0008152 GO:0008289 GO:0009791 GO:0009987 GO:0010008 GO:0010467 GO:0012505 GO:0016020 GO:0016043 GO:0016050 GO:0016192 GO:0019538 GO:0019898 GO:0030904 GO:0031090 GO:0031410 GO:0031902 GO:0031982 GO:0031984 GO:0032501 GO:0032502 GO:0032585 GO:0032588 GO:0032991 GO:0035091 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043621 GO:0044238 GO:0044422 GO:0044424 GO:0044425 GO:0044431 GO:0044433 GO:0044440 GO:0044444 GO:0044446 GO:0044464 GO:0045324 GO:0046907 GO:0046982 GO:0046983 GO:0048856 GO:0051179 GO:0051234 GO:0051604 GO:0051641 GO:0051649 GO:0071704 GO:0071840 GO:0090351 GO:0097708 GO:0098588 GO:0098657 GO:0098791 GO:0098796 GO:0098805 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04144 map04144

Pfam Domains

Protein Families

PX Vps5

Protein Analysis

586

Amino Acids

65.34

Weight (kDa)

5.53

Isoelectric Point (pI)

50.75

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PX	PF00787	193 - 274	4e-18	PX domain
Vps5	PF09325	343 - 575	1.7e-19	Vps5 C terminal like

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010972)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G07120 AT5G07120 AT5G07120 AT5G58440
fragaria_vesca	FvH4_3g39770
malus_domestica	MD03G1056600.v1.1 MD11G1058400.v1.1
prunus_persica	Prupe.6G048200_v2.0.a1
pyrus_communis	pycom11g04840
rosa_chinensis	RchiOBHm_Chr5g0071651
rosa_laevigata	RLG00000036238
rosa_multiflora	Rmu_sc0002060.1_g000020 Rmu_sc0005745.1_g000035 Rmu_sc0006567.1_g000005
rosa_roxburghii	Rroxscaffold_1G00009520
rosa_rugosa	Rorug05G0414000
rosa_samantha	Rh5BG489500 Rh5CG513300 Rh5DG500300
rosa_wichuraiana	Rw5G043660

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	808
Acc36I	ACCTGC	1 cut(s)	808
AccBSI	CCGCTC	1 cut(s)	1582
AccI	GTMKAC	1 cut(s)	1712
AccII	CGCG	3 cut(s)	47, 267, 433
AccIII	TCCGGA	1 cut(s)	568
AclI	AACGTT	1 cut(s)	623
AclWI	GGATC	3 cut(s)	233, 589, 1592
AcsI	RAATTY	3 cut(s)	505, 587, 1479
AcyI	GRCGYC	2 cut(s)	234, 860
AdeI	CACNNNGTG	2 cut(s)	781, 940
AfaI	GTAC	7 cut(s)	107, 263, 422, 458, 542, 650, 755
AfiI	CCNNNNNNNGG	6 cut(s)	286, 500, 575, 640, 956, 1037
AflIII	ACRYGT	1 cut(s)	1650
AgeI	ACCGGT	1 cut(s)	633
AgsI	TTSAA	9 cut(s)	805, 985, 994, 1225, 1463, 1469, 1613, 1648, 1657
AjiI	CACGTC	2 cut(s)	334, 616
AjnI	CCWGG	2 cut(s)	391, 837
AleI	CACNNNNGTG	1 cut(s)	854
AloI	GAACNNNNNNTCC	4 cut(s)	8, 40, 1205, 1237
Alw21I	GWGCWC	4 cut(s)	404, 419, 919, 1399
Alw26I	GTCTC	3 cut(s)	478, 1151, 1721
AlwI	GGATC	3 cut(s)	233, 589, 1592
Ama87I	CYCGRG	1 cut(s)	412
Aor13HI	TCCGGA	1 cut(s)	568
AoxI	GGCC	6 cut(s)	288, 674, 879, 1028, 1119, 1215
ApeKI	GCWGC	4 cut(s)	120, 1244, 1271, 1438
ApoI	RAATTY	3 cut(s)	505, 587, 1479
ArsI	GACNNNNNNTTYG	4 cut(s)	456, 488, 1694, 1726
AsiGI	ACCGGT	1 cut(s)	633
AspLEI	GCGC	3 cut(s)	49, 125, 931
AspS9I	GGNCC	2 cut(s)	289, 879
AsuC2I	CCSGG	4 cut(s)	20, 293, 519, 779
AsuHPI	GGTGA	3 cut(s)	715, 793, 1502
AvaI	CYCGRG	1 cut(s)	412
BanII	GRGCYC	5 cut(s)	11, 26, 404, 419, 1754
BauI	CACGAG	1 cut(s)	935
BbsI	GAAGAC	1 cut(s)	435
Bbv12I	GWGCWC	4 cut(s)	404, 419, 919, 1399
BbvI	GCAGC	4 cut(s)	132, 1231, 1258, 1425
BccI	CCATC	2 cut(s)	869, 1156
BceAI	ACGGC	4 cut(s)	109, 154, 394, 866
BciT130I	CCWGG	2 cut(s)	393, 839
BciVI	GTATCC	2 cut(s)	199, 1676
BclI	TGATCA	2 cut(s)	546, 783
BcnI	CCSGG	4 cut(s)	20, 293, 519, 779
BcoDI	GTCTC	3 cut(s)	478, 1151, 1721
BfaI	CTAG	4 cut(s)	282, 864, 1178, 1514
BfmI	CTRYAG	2 cut(s)	340, 1272
BfoI	RGCGCY	2 cut(s)	126, 932
BfuAI	ACCTGC	1 cut(s)	808
BfuI	GTATCC	2 cut(s)	199, 1676
BglII	AGATCT	1 cut(s)	1426
BisI	GCNGC	7 cut(s)	121, 366, 602, 1031, 1245, 1272, 1439
BlsI	GCNGC	7 cut(s)	122, 367, 603, 1032, 1246, 1273, 1440
BmcAI	AGTACT	1 cut(s)	755
Bme1390I	CCNGG	6 cut(s)	20, 293, 393, 519, 779, 839
BmeT110I	CYCGRG	1 cut(s)	412
BmgBI	CACGTC	2 cut(s)	334, 616
BmgT120I	GGNCC	2 cut(s)	289, 879
BmiI	GGNNCC	4 cut(s)	25, 291, 492, 880
BmrFI	CCNGG	6 cut(s)	20, 293, 393, 519, 779, 839
BmsI	GCATC	6 cut(s)	343, 390, 696, 858, 1118, 1540
BpiI	GAAGAC	1 cut(s)	435
BpmI	CTGGAG	1 cut(s)	1109
BpuEI	CTTGAG	1 cut(s)	778
BpuMI	CCSGG	4 cut(s)	20, 293, 519, 779
BsaAI	YACGTR	1 cut(s)	540
BsaHI	GRCGYC	2 cut(s)	234, 860
BsaI	GGTCTC	2 cut(s)	478, 1721
BsaJI	CCNNGG	5 cut(s)	19, 291, 376, 671, 837
BsaWI	WCCGGW	2 cut(s)	568, 633
BsaXI	ACNNNNNCTCC	4 cut(s)	8, 38, 1205, 1235
Bsc4I	CCNNNNNNNGG	6 cut(s)	286, 500, 575, 640, 956, 1037
Bse118I	RCCGGY	1 cut(s)	633
Bse1I	ACTGG	1 cut(s)	930
BseAI	TCCGGA	1 cut(s)	568
BseBI	CCWGG	2 cut(s)	393, 839
BseDI	CCNNGG	5 cut(s)	19, 291, 376, 671, 837
BseGI	GGATG	3 cut(s)	449, 873, 1555
BseLI	CCNNNNNNNGG	6 cut(s)	286, 500, 575, 640, 956, 1037
BseMII	CTCAG	3 cut(s)	1127, 1244, 1536
BseNI	ACTGG	1 cut(s)	930
BseRI	GAGGAG	5 cut(s)	166, 1058, 1226, 1532, 1739
BseXI	GCAGC	4 cut(s)	132, 1231, 1258, 1425
BsgI	GTGCAG	2 cut(s)	842, 1430
Bsh1236I	CGCG	3 cut(s)	47, 267, 433
Bsh1285I	CGRYCG	1 cut(s)	513
BshFI	GGCC	6 cut(s)	290, 676, 881, 1030, 1121, 1217
BshTI	ACCGGT	1 cut(s)	633
BsiEI	CGRYCG	1 cut(s)	513
BsiHKAI	GWGCWC	4 cut(s)	404, 419, 919, 1399
BsiHKCI	CYCGRG	1 cut(s)	412
BsiSI	CCGG	8 cut(s)	19, 293, 494, 518, 569, 598, 634, 779
BslFI	GGGAC	2 cut(s)	182, 626
BslI	CCNNNNNNNGG	6 cut(s)	286, 500, 575, 640, 956, 1037
BsmAI	GTCTC	3 cut(s)	478, 1151, 1721
BsmBI	CGTCTC	1 cut(s)	1151
BsmFI	GGGAC	2 cut(s)	182, 626
BsmI	GAATGC	1 cut(s)	1376
BsnI	GGCC	6 cut(s)	290, 676, 881, 1030, 1121, 1217
Bso31I	GGTCTC	2 cut(s)	478, 1721
BsoBI	CYCGRG	1 cut(s)	412
Bsp1286I	GDGCHC	7 cut(s)	11, 26, 404, 419, 919, 1399, 1754
Bsp13I	TCCGGA	1 cut(s)	568
Bsp68I	TCGCGA	1 cut(s)	433
BspANI	GGCC	6 cut(s)	290, 676, 881, 1030, 1121, 1217
BspCNI	CTCAG	4 cut(s)	4, 1126, 1243, 1537
BspEI	TCCGGA	1 cut(s)	568
BspFNI	CGCG	3 cut(s)	47, 267, 433
BspHI	TCATGA	1 cut(s)	1339
BspLI	GGNNCC	4 cut(s)	25, 291, 492, 880
BspMAI	CTGCAG	1 cut(s)	1276
BspMI	ACCTGC	1 cut(s)	808
BspPI	GGATC	3 cut(s)	233, 589, 1592
BspTNI	GGTCTC	2 cut(s)	478, 1721
BsrBI	CCGCTC	1 cut(s)	1582
BsrFI	RCCGGY	1 cut(s)	633
BsrI	ACTGG	1 cut(s)	930
BssAI	RCCGGY	1 cut(s)	633
BssECI	CCNNGG	5 cut(s)	19, 291, 376, 671, 837
BssNI	GRCGYC	2 cut(s)	234, 860
BssSI	CACGAG	1 cut(s)	935
Bst2BI	CACGAG	1 cut(s)	935
Bst2UI	CCWGG	2 cut(s)	393, 839
Bst4CI	ACNGT	5 cut(s)	261, 472, 1318, 1408, 1717
Bst6I	CTCTTC	2 cut(s)	730, 1422
BstACI	GRCGYC	2 cut(s)	234, 860
BstAPI	GCANNNNNTGC	1 cut(s)	1271
BstBAI	YACGTR	1 cut(s)	540
BstC8I	GCNNGC	5 cut(s)	951, 1119, 1242, 1285, 1635
BstDEI	CTNAG	4 cut(s)	12, 1113, 1230, 1545
BstDSI	CCRYGG	1 cut(s)	376
BstENI	CCTNNNNNAGG	1 cut(s)	954
BstF5I	GGATG	3 cut(s)	449, 873, 1555
BstFNI	CGCG	3 cut(s)	47, 267, 433
BstH2I	RGCGCY	2 cut(s)	126, 932
BstHHI	GCGC	3 cut(s)	49, 125, 931
BstMAI	GTCTC	3 cut(s)	478, 1151, 1721
BstMCI	CGRYCG	1 cut(s)	513
BstMWI	GCNNNNNNNGC	7 cut(s)	209, 362, 490, 829, 928, 1271, 1280
BstNI	CCWGG	2 cut(s)	393, 839
BstNSI	RCATGY	2 cut(s)	1637, 1654
BstSCI	CCNGG	6 cut(s)	18, 291, 391, 517, 777, 837
BstSFI	CTRYAG	2 cut(s)	340, 1272
BstUI	CGCG	3 cut(s)	47, 267, 433
BstV1I	GCAGC	4 cut(s)	132, 1231, 1258, 1425
BstV2I	GAAGAC	1 cut(s)	435
BstX2I	RGATCY	1 cut(s)	1426
BstXI	CCANNNNNNTGG	1 cut(s)	856
BstYI	RGATCY	1 cut(s)	1426
BsuI	GTATCC	2 cut(s)	199, 1676
BsuRI	GGCC	6 cut(s)	290, 676, 881, 1030, 1121, 1217
BtgI	CCRYGG	1 cut(s)	376
BtrI	CACGTC	2 cut(s)	334, 616
BtsCI	GGATG	3 cut(s)	449, 873, 1555
BtsIMutI	CAGTG	4 cut(s)	849, 923, 1413, 1542
BtuMI	TCGCGA	1 cut(s)	433
BveI	ACCTGC	1 cut(s)	808
Cac8I	GCNNGC	5 cut(s)	951, 1119, 1242, 1285, 1635
CciI	TCATGA	1 cut(s)	1339
CfoI	GCGC	3 cut(s)	49, 125, 931
Cfr10I	RCCGGY	1 cut(s)	633
Cfr13I	GGNCC	2 cut(s)	289, 879
CseI	GACGC	2 cut(s)	242, 849
Csp6I	GTAC	7 cut(s)	106, 262, 421, 457, 541, 649, 754
CspAI	ACCGGT	1 cut(s)	633
CviAII	CATG	7 cut(s)	127, 1106, 1150, 1252, 1340, 1634, 1651
CviQI	GTAC	7 cut(s)	106, 262, 421, 457, 541, 649, 754
DdeI	CTNAG	4 cut(s)	12, 1113, 1230, 1545
DraIII	CACNNNGTG	2 cut(s)	781, 940
Eam1104I	CTCTTC	2 cut(s)	730, 1422
EarI	CTCTTC	2 cut(s)	730, 1422
EciI	GGCGGA	3 cut(s)	594, 656, 909
Ecl136II	GAGCTC	2 cut(s)	402, 417
Eco147I	AGGCCT	1 cut(s)	1217
Eco24I	GRGCYC	5 cut(s)	11, 26, 404, 419, 1754
Eco31I	GGTCTC	2 cut(s)	478, 1721
Eco53kI	GAGCTC	2 cut(s)	402, 417
Eco88I	CYCGRG	1 cut(s)	412
EcoICRI	GAGCTC	2 cut(s)	402, 417
EcoNI	CCTNNNNNAGG	1 cut(s)	954
EcoRI	GAATTC	2 cut(s)	505, 587
EcoRII	CCWGG	2 cut(s)	391, 837
EcoT22I	ATGCAT	1 cut(s)	1111
EcoT38I	GRGCYC	5 cut(s)	11, 26, 404, 419, 1754
Esp3I	CGTCTC	1 cut(s)	1151
FaeI	CATG	7 cut(s)	130, 1109, 1153, 1255, 1343, 1637, 1654
FaqI	GGGAC	2 cut(s)	182, 626
FatI	CATG	7 cut(s)	126, 1105, 1149, 1251, 1339, 1633, 1650
FauI	CCCGC	5 cut(s)	208, 262, 324, 494, 1233
FbaI	TGATCA	2 cut(s)	546, 783
FblI	GTMKAC	1 cut(s)	1712
Fnu4HI	GCNGC	7 cut(s)	121, 366, 602, 1031, 1245, 1272, 1439
FokI	GGATG	3 cut(s)	436, 880, 1562
FriOI	GRGCYC	5 cut(s)	11, 26, 404, 419, 1754
Fsp4HI	GCNGC	7 cut(s)	121, 366, 602, 1031, 1245, 1272, 1439
FspBI	CTAG	4 cut(s)	282, 864, 1178, 1514
GlaI	GCGC	3 cut(s)	48, 124, 930
GluI	GCNGC	7 cut(s)	121, 366, 602, 1031, 1245, 1272, 1439
GsuI	CTGGAG	1 cut(s)	1109
HaeII	RGCGCY	2 cut(s)	126, 932
HaeIII	GGCC	6 cut(s)	290, 676, 881, 1030, 1121, 1217
HapII	CCGG	8 cut(s)	19, 293, 494, 518, 569, 598, 634, 779
HgaI	GACGC	2 cut(s)	242, 849
HhaI	GCGC	3 cut(s)	49, 125, 931
Hin1I	GRCGYC	2 cut(s)	234, 860
Hin1II	CATG	7 cut(s)	130, 1109, 1153, 1255, 1343, 1637, 1654
Hin6I	GCGC	3 cut(s)	47, 123, 929
HinP1I	GCGC	3 cut(s)	47, 123, 929
HincII	GTYRAC	1 cut(s)	1713
HindII	GTYRAC	1 cut(s)	1713
HindIII	AAGCTT	1 cut(s)	887
HpaII	CCGG	8 cut(s)	19, 293, 494, 518, 569, 598, 634, 779
HphI	GGTGA	3 cut(s)	715, 793, 1502
Hpy166II	GTNNAC	2 cut(s)	1607, 1713
Hpy8I	GTNNAC	2 cut(s)	1607, 1713
Hpy99I	CGWCG	8 cut(s)	32, 83, 136, 166, 169, 236, 335, 800
HpyAV	CCTTC	4 cut(s)	330, 481, 799, 1651
HpyCH4III	ACNGT	5 cut(s)	261, 472, 1318, 1408, 1717
HpyCH4IV	ACGT	5 cut(s)	333, 534, 539, 615, 623
HpyF10VI	GCNNNNNNNGC	7 cut(s)	209, 362, 490, 829, 928, 1271, 1280
HpyF3I	CTNAG	4 cut(s)	12, 1113, 1230, 1545
HpySE526I	ACGT	5 cut(s)	333, 534, 539, 615, 623
Hsp92I	GRCGYC	2 cut(s)	234, 860
Hsp92II	CATG	7 cut(s)	130, 1109, 1153, 1255, 1343, 1637, 1654
HspAI	GCGC	3 cut(s)	47, 123, 929
Kpn2I	TCCGGA	1 cut(s)	568
Ksp22I	TGATCA	2 cut(s)	546, 783
LmnI	GCTCC	8 cut(s)	29, 407, 490, 521, 727, 922, 1019, 1090
Lsp1109I	GCAGC	4 cut(s)	132, 1231, 1258, 1425
LweI	GCATC	6 cut(s)	343, 390, 696, 858, 1118, 1540
MaeI	CTAG	4 cut(s)	282, 864, 1178, 1514
MaeII	ACGT	5 cut(s)	333, 534, 539, 615, 623
MaeIII	GTNAC	3 cut(s)	535, 619, 1490
MbiI	CCGCTC	1 cut(s)	1582
MboII	GAAGA	6 cut(s)	68, 435, 747, 1087, 1267, 1409
MflI	RGATCY	1 cut(s)	1426
MhlI	GDGCHC	7 cut(s)	11, 26, 404, 419, 919, 1399, 1754
MluCI	AATT	6 cut(s)	112, 505, 529, 587, 800, 1479
MlyI	GAGTC	6 cut(s)	397, 547, 603, 653, 706, 1408
MmeI	TCCRAC	2 cut(s)	51, 429
Mph1103I	ATGCAT	1 cut(s)	1111
MroI	TCCGGA	1 cut(s)	568
MseI	TTAA	4 cut(s)	1134, 1188, 1304, 1523
MslI	CAYNNNNRTG	1 cut(s)	854
MspA1I	CMGCKG	1 cut(s)	1033
MspI	CCGG	8 cut(s)	19, 293, 494, 518, 569, 598, 634, 779
MspR9I	CCNGG	6 cut(s)	20, 293, 393, 519, 779, 839
Mva1269I	GAATGC	1 cut(s)	1376
MvaI	CCWGG	2 cut(s)	393, 839
MvnI	CGCG	3 cut(s)	47, 267, 433
MwoI	GCNNNNNNNGC	7 cut(s)	209, 362, 490, 829, 928, 1271, 1280
NciI	CCSGG	4 cut(s)	20, 293, 519, 779
NlaIII	CATG	7 cut(s)	130, 1109, 1153, 1255, 1343, 1637, 1654
NlaIV	GGNNCC	4 cut(s)	25, 291, 492, 880
NmeAIII	GCCGAG	2 cut(s)	266, 696
NmuCI	GTSAC	2 cut(s)	535, 1490
NruI	TCGCGA	1 cut(s)	433
NsiI	ATGCAT	1 cut(s)	1111
NspI	RCATGY	2 cut(s)	1637, 1654
OliI	CACNNNNGTG	1 cut(s)	854
PaeI	GCATGC	1 cut(s)	1637
PaeR7I	CTCGAG	1 cut(s)	412
PagI	TCATGA	1 cut(s)	1339
PaqCI	CACCTGC	1 cut(s)	808
PceI	AGGCCT	1 cut(s)	1217
PciI	ACATGT	1 cut(s)	1650
PcsI	WCGNNNNNNNCGW	4 cut(s)	78, 167, 437, 557
PctI	GAATGC	1 cut(s)	1376
PfeI	GAWTC	5 cut(s)	478, 895, 1124, 1290, 1504
PfoI	TCCNGGA	1 cut(s)	517
PinAI	ACCGGT	1 cut(s)	633
PkrI	GCNGC	7 cut(s)	122, 367, 603, 1032, 1246, 1273, 1440
Ple19I	CGATCG	1 cut(s)	513
PleI	GAGTC	6 cut(s)	396, 547, 602, 652, 705, 1408
PpsI	GAGTC	6 cut(s)	396, 547, 602, 652, 705, 1408
Ppu21I	YACGTR	1 cut(s)	540
PscI	ACATGT	1 cut(s)	1650
Psp124BI	GAGCTC	2 cut(s)	404, 419
Psp1406I	AACGTT	1 cut(s)	623
Psp6I	CCWGG	2 cut(s)	391, 837
PspGI	CCWGG	2 cut(s)	391, 837
PspN4I	GGNNCC	4 cut(s)	25, 291, 492, 880
PspPI	GGNCC	2 cut(s)	289, 879
PspXI	VCTCGAGB	1 cut(s)	412
PsrI	GAACNNNNNNTAC	2 cut(s)	641, 673
PstI	CTGCAG	1 cut(s)	1276
PsuI	RGATCY	1 cut(s)	1426
PvuI	CGATCG	1 cut(s)	513
RruI	TCGCGA	1 cut(s)	433
RsaI	GTAC	7 cut(s)	107, 263, 422, 458, 542, 650, 755
RsaNI	GTAC	7 cut(s)	106, 262, 421, 457, 541, 649, 754
RseI	CAYNNNNRTG	1 cut(s)	854
SacI	GAGCTC	2 cut(s)	404, 419
SalI	GTCGAC	1 cut(s)	1711
SaqAI	TTAA	4 cut(s)	1134, 1188, 1304, 1523
SatI	GCNGC	7 cut(s)	121, 366, 602, 1031, 1245, 1272, 1439
Sau96I	GGNCC	2 cut(s)	289, 879
ScaI	AGTACT	1 cut(s)	755
SchI	GAGTC	6 cut(s)	397, 547, 603, 653, 706, 1408
ScrFI	CCNGG	6 cut(s)	20, 293, 393, 519, 779, 839
SduI	GDGCHC	7 cut(s)	11, 26, 404, 419, 919, 1399, 1754
SfaNI	GCATC	6 cut(s)	343, 390, 696, 858, 1118, 1540
SfcI	CTRYAG	2 cut(s)	340, 1272
Sfr274I	CTCGAG	1 cut(s)	412
SlaI	CTCGAG	1 cut(s)	412
SmiMI	CAYNNNNRTG	1 cut(s)	854
SmlI	CTYRAG	2 cut(s)	412, 757
SmoI	CTYRAG	2 cut(s)	412, 757
SphI	GCATGC	1 cut(s)	1637
Sse9I	AATT	6 cut(s)	112, 505, 529, 587, 800, 1479
SseBI	AGGCCT	1 cut(s)	1217
SspMI	CTAG	4 cut(s)	282, 864, 1178, 1514
SstI	GAGCTC	2 cut(s)	404, 419
StuI	AGGCCT	1 cut(s)	1217
StyD4I	CCNGG	6 cut(s)	18, 291, 391, 517, 777, 837
TaaI	ACNGT	5 cut(s)	261, 472, 1318, 1408, 1717
TaiI	ACGT	5 cut(s)	336, 537, 542, 618, 626
TasI	AATT	6 cut(s)	112, 505, 529, 587, 800, 1479
TatI	WGTACW	2 cut(s)	456, 753
TauI	GCSGC	3 cut(s)	368, 604, 1033
TfiI	GAWTC	5 cut(s)	478, 895, 1124, 1290, 1504
Tru1I	TTAA	4 cut(s)	1134, 1188, 1304, 1523
Tru9I	TTAA	4 cut(s)	1134, 1188, 1304, 1523
TscAI	CASTG	4 cut(s)	856, 930, 1413, 1549
TseFI	GTSAC	2 cut(s)	535, 1490
TseI	GCWGC	4 cut(s)	120, 1244, 1271, 1438
Tsp45I	GTSAC	2 cut(s)	535, 1490
TspDTI	ATGAA	6 cut(s)	1094, 1094, 1268, 1328, 1356, 1542
TspGWI	ACGGA	1 cut(s)	429
TspRI	CASTG	4 cut(s)	856, 930, 1413, 1549
XagI	CCTNNNNNAGG	1 cut(s)	954
XapI	RAATTY	3 cut(s)	505, 587, 1479
XceI	RCATGY	2 cut(s)	1637, 1654
XhoI	CTCGAG	1 cut(s)	412
XmiI	GTMKAC	1 cut(s)	1712
XspI	CTAG	4 cut(s)	282, 864, 1178, 1514
ZrmI	AGTACT	1 cut(s)	755
Zsp2I	ATGCAT	1 cut(s)	1111

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.