MD03G1059100.v1.1

Biogenesis of lysosome-related organelles complex 1 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008067
Physical Location & Seq
Reverse (-)
4761035 .. 4761888
854 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1059100.v1.1.491

Sequence Viewer

Length: 453 bp
ATGCACTCACAAACGTCTCTTCCACTGGCCCATGGCGCCGTCGCATCGCCGTCAACCTTCTCGTTCTCGTCGGAGCCAGAGCCGGGAGGTGTAGAAGAAGCTCTGCTCCAGCTCGTCCAGGATCACCACCACGCCTCCCTCAGACTCCGCGACGCCACCGGCGAAGAAGGACGCGATCAAGAAGGCAGCGCGTGCGGCGGACCTGATGGTGGAGGCGGTGAACGGCGGCGCGTGCAAGAGGCGTTCGTAAACGAGAAGCGAATCGAGTATGAAATTAGGGCTTTGGCTGCCACCATTGCACGATTCAGCAAACAGACCGATCAGTCGCTTTCTGTCACTCACTCTATGAACACTGCCATCAAGGAAATTGGAGATTTTGAGAACTGGATGAAGATCATGGAATTTGATTGTAAAAGCATCACTGCAGCCATCCACAATATTCACCAAGCATGA

Protein Analysis

151

Amino Acids

16.37

Weight (kDa)

5.42

Isoelectric Point (pI)

68.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCN5L1 PF06320 75 - 149 2e-26 GCN5-like protein 1 (GCN5L1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 322
AccB1I GGYRCC 1 cut(s) 35
AccII CGCG 4 cut(s) 150, 174, 191, 231
AciI CCGC 5 cut(s) 148, 195, 198, 216, 226
AclWI GGATC 1 cut(s) 129
AcsI RAATTY 1 cut(s) 401
AcyI GRCGYC 2 cut(s) 36, 153
AfiI CCNNNNNNNGG 2 cut(s) 83, 209
AjnI CCWGG 1 cut(s) 117
AluBI AGCT 2 cut(s) 101, 112
AluI AGCT 2 cut(s) 101, 112
Alw26I GTCTC 1 cut(s) 21
AlwI GGATC 1 cut(s) 129
AoxI GGCC 1 cut(s) 27
ApeKI GCWGC 3 cut(s) 186, 287, 425
ApoI RAATTY 1 cut(s) 401
AspLEI GCGC 3 cut(s) 38, 191, 231
AspS9I GGNCC 2 cut(s) 28, 200
AsuC2I CCSGG 1 cut(s) 84
AsuHPI GGTGA 3 cut(s) 116, 230, 434
AvaII GGWCC 1 cut(s) 200
BanI GGYRCC 1 cut(s) 35
BbvI GCAGC 3 cut(s) 198, 274, 437
BccI CCATC 3 cut(s) 200, 365, 437
BceAI ACGGC 3 cut(s) 23, 34, 239
BciT130I CCWGG 1 cut(s) 119
BcnI CCSGG 1 cut(s) 84
BcoDI GTCTC 1 cut(s) 21
BfmI CTRYAG 1 cut(s) 423
BfoI RGCGCY 1 cut(s) 39
BisI GCNGC 5 cut(s) 187, 196, 227, 288, 426
BlsI GCNGC 5 cut(s) 188, 197, 228, 289, 427
Bme1390I CCNGG 2 cut(s) 84, 119
Bme18I GGWCC 1 cut(s) 200
BmgT120I GGNCC 2 cut(s) 28, 200
BmiI GGNNCC 2 cut(s) 37, 75
BmrFI CCNGG 2 cut(s) 84, 119
BmsI GCATC 2 cut(s) 53, 426
BpmI CTGGAG 1 cut(s) 92
BpuMI CCSGG 1 cut(s) 84
BsaBI GATNNNNATC 1 cut(s) 392
BsaHI GRCGYC 2 cut(s) 36, 153
BsaJI CCNNGG 1 cut(s) 31
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 2 cut(s) 83, 209
Bse118I RCCGGY 1 cut(s) 158
Bse1I ACTGG 2 cut(s) 30, 389
Bse3DI GCAATG 1 cut(s) 294
Bse8I GATNNNNATC 1 cut(s) 392
BseBI CCWGG 1 cut(s) 119
BseDI CCNNGG 1 cut(s) 31
BseGI GGATG 2 cut(s) 393, 429
BseJI GATNNNNATC 1 cut(s) 392
BseLI CCNNNNNNNGG 2 cut(s) 83, 209
BseMI GCAATG 1 cut(s) 294
BseMII CTCAG 1 cut(s) 154
BseNI ACTGG 2 cut(s) 30, 389
BseXI GCAGC 3 cut(s) 198, 274, 437
Bsh1236I CGCG 4 cut(s) 150, 174, 191, 231
BshFI GGCC 1 cut(s) 29
BshNI GGYRCC 1 cut(s) 35
BsiSI CCGG 2 cut(s) 83, 159
BslI CCNNNNNNNGG 2 cut(s) 83, 209
BsmAI GTCTC 1 cut(s) 21
BsmBI CGTCTC 1 cut(s) 21
BsnI GGCC 1 cut(s) 29
Bsp143I GATC 4 cut(s) 121, 175, 319, 393
Bsp19I CCATGG 1 cut(s) 31
BspACI CCGC 5 cut(s) 148, 195, 198, 216, 226
BspANI GGCC 1 cut(s) 29
BspCNI CTCAG 1 cut(s) 153
BspFNI CGCG 4 cut(s) 150, 174, 191, 231
BspLI GGNNCC 2 cut(s) 37, 75
BspMAI CTGCAG 1 cut(s) 427
BspPI GGATC 1 cut(s) 129
BspT107I GGYRCC 1 cut(s) 35
BsrDI GCAATG 1 cut(s) 294
BsrFI RCCGGY 1 cut(s) 158
BsrI ACTGG 2 cut(s) 30, 389
BssAI RCCGGY 1 cut(s) 158
BssECI CCNNGG 1 cut(s) 31
BssMI GATC 4 cut(s) 121, 175, 319, 393
BssNI GRCGYC 2 cut(s) 36, 153
BssT1I CCWWGG 1 cut(s) 31
Bst2UI CCWGG 1 cut(s) 119
Bst6I CTCTTC 1 cut(s) 24
BstACI GRCGYC 2 cut(s) 36, 153
BstAPI GCANNNNNTGC 1 cut(s) 192
BstC8I GCNNGC 2 cut(s) 193, 233
BstDEI CTNAG 1 cut(s) 140
BstDSI CCRYGG 1 cut(s) 31
BstF5I GGATG 2 cut(s) 393, 429
BstFNI CGCG 4 cut(s) 150, 174, 191, 231
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 3 cut(s) 38, 191, 231
BstKTI GATC 4 cut(s) 124, 178, 322, 396
BstMAI GTCTC 1 cut(s) 21
BstMBI GATC 4 cut(s) 121, 175, 319, 393
BstMWI GCNNNNNNNGC 6 cut(s) 35, 192, 195, 232, 287, 296
BstNI CCWGG 1 cut(s) 119
BstSCI CCNGG 2 cut(s) 82, 117
BstSFI CTRYAG 1 cut(s) 423
BstUI CGCG 4 cut(s) 150, 174, 191, 231
BstV1I GCAGC 3 cut(s) 198, 274, 437
BsuRI GGCC 1 cut(s) 29
BtgI CCRYGG 1 cut(s) 31
BtgZI GCGATG 1 cut(s) 30
BtsCI GGATG 2 cut(s) 393, 429
BtsI GCAGTG 2 cut(s) 351, 420
BtsIMutI CAGTG 3 cut(s) 23, 351, 420
Cac8I GCNNGC 2 cut(s) 193, 233
CfoI GCGC 3 cut(s) 38, 191, 231
Cfr10I RCCGGY 1 cut(s) 158
Cfr13I GGNCC 2 cut(s) 28, 200
CseI GACGC 2 cut(s) 161, 180
CviAII CATG 3 cut(s) 32, 397, 450
CviJI RGCY 8 cut(s) 29, 76, 82, 101, 112, 281, 287, 428
CviKI_1 RGCY 8 cut(s) 29, 76, 82, 101, 112, 281, 287, 428
DdeI CTNAG 1 cut(s) 140
DinI GGCGCC 1 cut(s) 37
DpnI GATC 4 cut(s) 123, 177, 321, 395
DpnII GATC 4 cut(s) 121, 175, 319, 393
DrdI GACNNNNNNGTC 1 cut(s) 322
DseDI GACNNNNNNGTC 1 cut(s) 322
Eam1104I CTCTTC 1 cut(s) 24
EarI CTCTTC 1 cut(s) 24
EciI GGCGGA 1 cut(s) 213
Eco130I CCWWGG 1 cut(s) 31
Eco47I GGWCC 1 cut(s) 200
EcoRII CCWGG 1 cut(s) 117
EcoT14I CCWWGG 1 cut(s) 31
EgeI GGCGCC 1 cut(s) 37
EheI GGCGCC 1 cut(s) 37
ErhI CCWWGG 1 cut(s) 31
Esp3I CGTCTC 1 cut(s) 21
FaeI CATG 3 cut(s) 35, 400, 453
FaiI YATR 5 cut(s) 33, 270, 347, 398, 451
FatI CATG 3 cut(s) 31, 396, 449
Fnu4HI GCNGC 5 cut(s) 187, 196, 227, 288, 426
FokI GGATG 2 cut(s) 400, 416
Fsp4HI GCNGC 5 cut(s) 187, 196, 227, 288, 426
GlaI GCGC 3 cut(s) 37, 190, 230
GluI GCNGC 5 cut(s) 187, 196, 227, 288, 426
GsuI CTGGAG 1 cut(s) 92
HaeII RGCGCY 1 cut(s) 39
HaeIII GGCC 1 cut(s) 29
HapII CCGG 2 cut(s) 83, 159
HgaI GACGC 2 cut(s) 161, 180
HhaI GCGC 3 cut(s) 38, 191, 231
Hin1I GRCGYC 2 cut(s) 36, 153
Hin1II CATG 3 cut(s) 35, 400, 453
Hin6I GCGC 3 cut(s) 36, 189, 229
HinP1I GCGC 3 cut(s) 36, 189, 229
HincII GTYRAC 1 cut(s) 54
HindII GTYRAC 1 cut(s) 54
HinfI GANTC 3 cut(s) 144, 261, 303
HpaII CCGG 2 cut(s) 83, 159
HphI GGTGA 3 cut(s) 116, 230, 434
Hpy166II GTNNAC 3 cut(s) 54, 221, 250
Hpy188I TCNGA 2 cut(s) 73, 143
Hpy188III TCNNGA 1 cut(s) 179
Hpy8I GTNNAC 3 cut(s) 54, 221, 250
Hpy99I CGWCG 3 cut(s) 44, 73, 155
HpyAV CCTTC 3 cut(s) 67, 161, 176
HpyCH4IV ACGT 1 cut(s) 14
HpyCH4V TGCA 4 cut(s) 4, 235, 299, 425
HpyF10VI GCNNNNNNNGC 6 cut(s) 35, 192, 195, 232, 287, 296
HpyF3I CTNAG 1 cut(s) 140
HpySE526I ACGT 1 cut(s) 14
Hsp92I GRCGYC 2 cut(s) 36, 153
Hsp92II CATG 3 cut(s) 35, 400, 453
HspAI GCGC 3 cut(s) 36, 189, 229
KasI GGCGCC 1 cut(s) 35
Kzo9I GATC 4 cut(s) 121, 175, 319, 393
LmnI GCTCC 2 cut(s) 73, 111
LpnPI CCDG 9 cut(s) 11, 90, 96, 104, 122, 131, 172, 216, 370
Lsp1109I GCAGC 3 cut(s) 198, 274, 437
LweI GCATC 2 cut(s) 53, 426
MaeII ACGT 1 cut(s) 14
MaeIII GTNAC 1 cut(s) 334
MalI GATC 4 cut(s) 123, 177, 321, 395
MboI GATC 4 cut(s) 121, 175, 319, 393
MboII GAAGA 4 cut(s) 11, 107, 176, 403
MluCI AATT 3 cut(s) 273, 366, 401
Mly113I GGCGCC 1 cut(s) 36
MlyI GAGTC 1 cut(s) 138
MmeI TCCRAC 1 cut(s) 51
MnlI CCTC 5 cut(s) 80, 145, 149, 206, 232
MspI CCGG 2 cut(s) 83, 159
MspR9I CCNGG 2 cut(s) 84, 119
MvaI CCWGG 1 cut(s) 119
MvnI CGCG 4 cut(s) 150, 174, 191, 231
MwoI GCNNNNNNNGC 6 cut(s) 35, 192, 195, 232, 287, 296
NarI GGCGCC 1 cut(s) 36
NciI CCSGG 1 cut(s) 84
NcoI CCATGG 1 cut(s) 31
NdeII GATC 4 cut(s) 121, 175, 319, 393
NlaIII CATG 3 cut(s) 35, 400, 453
NlaIV GGNNCC 2 cut(s) 37, 75
NmuCI GTSAC 1 cut(s) 334
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PfeI GAWTC 2 cut(s) 261, 303
PfoI TCCNGGA 1 cut(s) 117
PkrI GCNGC 5 cut(s) 188, 197, 228, 289, 427
PleI GAGTC 1 cut(s) 138
PluTI GGCGCC 1 cut(s) 39
PpsI GAGTC 1 cut(s) 138
Psp6I CCWGG 1 cut(s) 117
PspGI CCWGG 1 cut(s) 117
PspN4I GGNNCC 2 cut(s) 37, 75
PspPI GGNCC 2 cut(s) 28, 200
PstI CTGCAG 1 cut(s) 427
SatI GCNGC 5 cut(s) 187, 196, 227, 288, 426
Sau3AI GATC 4 cut(s) 121, 175, 319, 393
Sau96I GGNCC 2 cut(s) 28, 200
SchI GAGTC 1 cut(s) 138
ScrFI CCNGG 2 cut(s) 84, 119
SetI ASST 6 cut(s) 17, 59, 91, 103, 114, 205
SfaNI GCATC 2 cut(s) 53, 426
SfcI CTRYAG 1 cut(s) 423
SfoI GGCGCC 1 cut(s) 37
SgrAI CRCCGGYG 1 cut(s) 158
SinI GGWCC 1 cut(s) 200
Sse9I AATT 3 cut(s) 273, 366, 401
SsiI CCGC 5 cut(s) 148, 195, 198, 216, 226
SspDI GGCGCC 1 cut(s) 35
SspI AATATT 1 cut(s) 439
StyD4I CCNGG 2 cut(s) 82, 117
StyI CCWWGG 1 cut(s) 31
TaiI ACGT 1 cut(s) 17
TaqI TCGA 1 cut(s) 264
TaqII GACCGA 1 cut(s) 332
TasI AATT 3 cut(s) 273, 366, 401
TauI GCSGC 2 cut(s) 198, 229
TfiI GAWTC 2 cut(s) 261, 303
TscAI CASTG 3 cut(s) 30, 358, 427
TseFI GTSAC 1 cut(s) 334
TseI GCWGC 3 cut(s) 186, 287, 425
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 3 cut(s) 285, 362, 404
TspRI CASTG 3 cut(s) 30, 358, 427
VpaK11BI GGWCC 1 cut(s) 200
XapI RAATTY 1 cut(s) 401
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.