Rw5G044020

Biogenesis of lysosome-related organelles complex 1 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008067
Physical Location & Seq
Reverse (-)
77361197 .. 77362293
1097 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G044020.1

Sequence Viewer

Length: 450 bp
ATGTACACACAATCTTCTCTTCCACTCGCCCAAGTCGGCGTGCCGTCGACGTCGTCATTCTTGCCGGAGCCGGAACCAGGTAGCATAGAAGACGCTCTGCTCCAATTGGTCCAGGACCACCACCACAACTCCCTCAAGCTTCGCGAACGCACAGATAGGGCGAAGAAGGACGCGATCAAGAAGGCGGCGCGTGCGGCGGAGCTGATGGTGGAGGCGGTGAACGGCGGCGTTCAAGAGTCGTTCGTAAACGAGAAGCGAATCGAGCTTGAGATTCGGGCTTTGGCGGCCACCATTACCCGATTCACCAAGCAAACCGATCAGTGGCTCACCGTCACTCATTCTATGAACACTGCCATCAAGGAAATTGGGGACTTTGAGAATTGGATGAAGATCATGGAGTTTGATTGTAAGAGCATCACTGCAGCTATACTCAATATTCACCAAGCTTGA

Protein Analysis

149

Amino Acids

16.7

Weight (kDa)

5.95

Isoelectric Point (pI)

49.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCN5L1 PF06320 36 - 148 1.9e-39 GCN5-like protein 1 (GCN5L1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 53
AccI GTMKAC 1 cut(s) 47
AccII CGCG 3 cut(s) 144, 173, 190
AciI CCGC 6 cut(s) 185, 194, 197, 215, 225, 284
AcoI YGGCCR 1 cut(s) 285
AcyI GRCGYC 1 cut(s) 50
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 2 cut(s) 77, 321
AgsI TTSAA 1 cut(s) 233
AjnI CCWGG 2 cut(s) 76, 111
AluBI AGCT 5 cut(s) 139, 202, 265, 425, 446
AluI AGCT 5 cut(s) 139, 202, 265, 425, 446
AoxI GGCC 1 cut(s) 285
ApeKI GCWGC 1 cut(s) 422
AspLEI GCGC 1 cut(s) 190
AspS9I GGNCC 2 cut(s) 109, 115
AsuHPI GGTGA 4 cut(s) 229, 295, 319, 431
AvaII GGWCC 2 cut(s) 109, 115
BbsI GAAGAC 1 cut(s) 96
BbvI GCAGC 1 cut(s) 434
BccI CCATC 2 cut(s) 199, 362
BceAI ACGGC 2 cut(s) 28, 238
BciT130I CCWGG 2 cut(s) 78, 113
BfmI CTRYAG 1 cut(s) 420
BisI GCNGC 5 cut(s) 186, 195, 226, 285, 423
BlsI GCNGC 5 cut(s) 187, 196, 227, 286, 424
Bme1390I CCNGG 2 cut(s) 78, 113
Bme18I GGWCC 2 cut(s) 109, 115
BmgT120I GGNCC 2 cut(s) 109, 115
BmiI GGNNCC 2 cut(s) 69, 75
BmrFI CCNGG 2 cut(s) 78, 113
BmsI GCATC 1 cut(s) 423
BpiI GAAGAC 1 cut(s) 96
BpuEI CTTGAG 2 cut(s) 119, 287
BsaBI GATNNNNATC 1 cut(s) 389
BsaHI GRCGYC 1 cut(s) 50
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 2 cut(s) 77, 321
Bse8I GATNNNNATC 1 cut(s) 389
BseBI CCWGG 2 cut(s) 78, 113
BseGI GGATG 1 cut(s) 390
BseJI GATNNNNATC 1 cut(s) 389
BseLI CCNNNNNNNGG 2 cut(s) 77, 321
BseXI GCAGC 1 cut(s) 434
Bsh1236I CGCG 3 cut(s) 144, 173, 190
BshFI GGCC 1 cut(s) 287
BsiSI CCGG 2 cut(s) 65, 71
BslFI GGGAC 1 cut(s) 383
BslI CCNNNNNNNGG 2 cut(s) 77, 321
BsmFI GGGAC 1 cut(s) 383
BsnI GGCC 1 cut(s) 287
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 3 cut(s) 174, 316, 390
Bsp68I TCGCGA 1 cut(s) 144
BspACI CCGC 6 cut(s) 185, 194, 197, 215, 225, 284
BspANI GGCC 1 cut(s) 287
BspFNI CGCG 3 cut(s) 144, 173, 190
BspLI GGNNCC 2 cut(s) 69, 75
BspMAI CTGCAG 1 cut(s) 424
BsrGI TGTACA 1 cut(s) 3
BssMI GATC 3 cut(s) 174, 316, 390
BssNI GRCGYC 1 cut(s) 50
Bst2UI CCWGG 2 cut(s) 78, 113
Bst4CI ACNGT 1 cut(s) 331
Bst6I CTCTTC 1 cut(s) 24
BstACI GRCGYC 1 cut(s) 50
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 41, 192
BstF5I GGATG 1 cut(s) 390
BstFNI CGCG 3 cut(s) 144, 173, 190
BstHHI GCGC 1 cut(s) 190
BstKTI GATC 3 cut(s) 177, 319, 393
BstMBI GATC 3 cut(s) 174, 316, 390
BstMWI GCNNNNNNNGC 4 cut(s) 191, 194, 262, 284
BstNI CCWGG 2 cut(s) 78, 113
BstSCI CCNGG 2 cut(s) 76, 111
BstSFI CTRYAG 1 cut(s) 420
BstUI CGCG 3 cut(s) 144, 173, 190
BstV1I GCAGC 1 cut(s) 434
BstV2I GAAGAC 1 cut(s) 96
BsuRI GGCC 1 cut(s) 287
BtsCI GGATG 1 cut(s) 390
BtsI GCAGTG 2 cut(s) 348, 417
BtsIMutI CAGTG 3 cut(s) 326, 348, 417
BtuMI TCGCGA 1 cut(s) 144
Cac8I GCNNGC 2 cut(s) 41, 192
CfoI GCGC 1 cut(s) 190
Cfr13I GGNCC 2 cut(s) 109, 115
CseI GACGC 2 cut(s) 101, 179
CsiI ACCWGGT 1 cut(s) 76
Csp6I GTAC 1 cut(s) 4
CviAII CATG 1 cut(s) 394
CviJI RGCY 9 cut(s) 70, 139, 202, 265, 278, 287, 325, 425, 446
CviKI_1 RGCY 9 cut(s) 70, 139, 202, 265, 278, 287, 325, 425, 446
CviQI GTAC 1 cut(s) 4
DpnI GATC 3 cut(s) 176, 318, 392
DpnII GATC 3 cut(s) 174, 316, 390
EaeI YGGCCR 1 cut(s) 285
Eam1104I CTCTTC 1 cut(s) 24
EarI CTCTTC 1 cut(s) 24
EciI GGCGGA 1 cut(s) 212
Eco47I GGWCC 2 cut(s) 109, 115
EcoRII CCWGG 2 cut(s) 76, 111
FaeI CATG 1 cut(s) 397
FaiI YATR 4 cut(s) 86, 344, 395, 428
FaqI GGGAC 1 cut(s) 383
FatI CATG 1 cut(s) 393
FblI GTMKAC 1 cut(s) 47
Fnu4HI GCNGC 5 cut(s) 186, 195, 226, 285, 423
FokI GGATG 1 cut(s) 397
Fsp4HI GCNGC 5 cut(s) 186, 195, 226, 285, 423
GlaI GCGC 1 cut(s) 189
GluI GCNGC 5 cut(s) 186, 195, 226, 285, 423
HaeIII GGCC 1 cut(s) 287
HapII CCGG 2 cut(s) 65, 71
HgaI GACGC 2 cut(s) 101, 179
HhaI GCGC 1 cut(s) 190
Hin1I GRCGYC 1 cut(s) 50
Hin1II CATG 1 cut(s) 397
Hin6I GCGC 1 cut(s) 188
HinP1I GCGC 1 cut(s) 188
HincII GTYRAC 1 cut(s) 48
HindII GTYRAC 1 cut(s) 48
HindIII AAGCTT 2 cut(s) 137, 444
HinfI GANTC 4 cut(s) 236, 258, 271, 300
HpaII CCGG 2 cut(s) 65, 71
HphI GGTGA 4 cut(s) 229, 295, 319, 431
Hpy166II GTNNAC 4 cut(s) 6, 48, 220, 247
Hpy188III TCNNGA 3 cut(s) 143, 178, 233
Hpy8I GTNNAC 4 cut(s) 6, 48, 220, 247
Hpy99I CGWCG 3 cut(s) 49, 52, 55
HpyAV CCTTC 2 cut(s) 160, 175
HpyCH4III ACNGT 1 cut(s) 331
HpyCH4IV ACGT 1 cut(s) 50
HpyCH4V TGCA 1 cut(s) 422
HpyF10VI GCNNNNNNNGC 4 cut(s) 191, 194, 262, 284
HpySE526I ACGT 1 cut(s) 50
Hsp92I GRCGYC 1 cut(s) 50
Hsp92II CATG 1 cut(s) 397
HspAI GCGC 1 cut(s) 188
Kzo9I GATC 3 cut(s) 174, 316, 390
LmnI GCTCC 3 cut(s) 67, 105, 199
LpnPI CCDG 6 cut(s) 63, 78, 84, 90, 98, 125
Lsp1109I GCAGC 1 cut(s) 434
LweI GCATC 1 cut(s) 423
MabI ACCWGGT 1 cut(s) 76
MaeII ACGT 1 cut(s) 50
MaeIII GTNAC 1 cut(s) 331
MalI GATC 3 cut(s) 176, 318, 392
MboI GATC 3 cut(s) 174, 316, 390
MboII GAAGA 5 cut(s) 6, 11, 101, 175, 400
MfeI CAATTG 1 cut(s) 104
MluCI AATT 3 cut(s) 104, 363, 379
MlyI GAGTC 1 cut(s) 245
MnlI CCTC 2 cut(s) 143, 205
MspI CCGG 2 cut(s) 65, 71
MspR9I CCNGG 2 cut(s) 78, 113
MunI CAATTG 1 cut(s) 104
MvaI CCWGG 2 cut(s) 78, 113
MvnI CGCG 3 cut(s) 144, 173, 190
MwoI GCNNNNNNNGC 4 cut(s) 191, 194, 262, 284
NdeII GATC 3 cut(s) 174, 316, 390
NlaIII CATG 1 cut(s) 397
NlaIV GGNNCC 2 cut(s) 69, 75
NmuCI GTSAC 1 cut(s) 331
NruI TCGCGA 1 cut(s) 144
PfeI GAWTC 3 cut(s) 258, 271, 300
PflFI GACNNNGTC 1 cut(s) 52
PfoI TCCNGGA 1 cut(s) 111
PkrI GCNGC 5 cut(s) 187, 196, 227, 286, 424
PleI GAGTC 1 cut(s) 244
PpsI GAGTC 1 cut(s) 244
Psp6I CCWGG 2 cut(s) 76, 111
PspGI CCWGG 2 cut(s) 76, 111
PspN4I GGNNCC 2 cut(s) 69, 75
PspPI GGNCC 2 cut(s) 109, 115
PstI CTGCAG 1 cut(s) 424
PsyI GACNNNGTC 1 cut(s) 52
RruI TCGCGA 1 cut(s) 144
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
SalI GTCGAC 1 cut(s) 46
SatI GCNGC 5 cut(s) 186, 195, 226, 285, 423
Sau3AI GATC 3 cut(s) 174, 316, 390
Sau96I GGNCC 2 cut(s) 109, 115
SchI GAGTC 1 cut(s) 245
ScrFI CCNGG 2 cut(s) 78, 113
SetI ASST 7 cut(s) 53, 82, 141, 204, 267, 427, 448
SexAI ACCWGGT 1 cut(s) 76
SfaNI GCATC 1 cut(s) 423
SfcI CTRYAG 1 cut(s) 420
SgrDI CGTCGACG 1 cut(s) 46
SinI GGWCC 2 cut(s) 109, 115
SmlI CTYRAG 2 cut(s) 134, 266
SmoI CTYRAG 2 cut(s) 134, 266
Sse9I AATT 3 cut(s) 104, 363, 379
SsiI CCGC 6 cut(s) 185, 194, 197, 215, 225, 284
SspI AATATT 1 cut(s) 436
StyD4I CCNGG 2 cut(s) 76, 111
TaaI ACNGT 1 cut(s) 331
TaiI ACGT 1 cut(s) 53
TaqI TCGA 2 cut(s) 47, 261
TasI AATT 3 cut(s) 104, 363, 379
TatI WGTACW 1 cut(s) 3
TauI GCSGC 4 cut(s) 188, 197, 228, 287
TfiI GAWTC 3 cut(s) 258, 271, 300
TscAI CASTG 3 cut(s) 326, 355, 424
TseFI GTSAC 1 cut(s) 331
TseI GCWGC 1 cut(s) 422
Tsp45I GTSAC 1 cut(s) 331
TspDTI ATGAA 2 cut(s) 359, 401
TspRI CASTG 3 cut(s) 326, 355, 424
Tth111I GACNNNGTC 1 cut(s) 52
VpaK11BI GGWCC 2 cut(s) 109, 115
XmiI GTMKAC 1 cut(s) 47
ZraI GACGTC 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.