Rh5AG474100

Biogenesis of lysosome-related organelles complex 1 subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0008067
Physical Location & Seq
Reverse (-)
81376088 .. 81378177
2090 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG474100.1

Sequence Viewer

Length: 405 bp
ATGTACACACAATCTTCTCTTCCACTCGCCCAAGTCGGCGTGCCGTCGACGTCGTCATTCTTGCCGGAGCCGGAACCAGGTAGCATAGAGGACGCTCTGCTCCAATTGGTCCAGGACCACCACCACAACTCCCTCAAGCTTCGCGAACGCACAGAAGATAGGGCGAAGAAGGACGCGATCAAGAAGGCGGCGCGTGCGGCGGAGCTGATGGTGGAGGCGGTGAACGGCGGCGTTCAAGAGTCGTTCGTAAACGAGAAGCGAATCGAGCTTGAGATTCGGGCTTTGGCGGCCACCATTACCCGATTCACCAAGCAAACGGATCAGTGGCTCACCGTCACTCATTCTATGAACACTGCCATCAAGGTAGAGACTTTTAATTTTTTATTTTTTCCGATTTTGTTGTGA

Protein Analysis

134

Amino Acids

15.06

Weight (kDa)

6.08

Isoelectric Point (pI)

51.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GCN5L1 PF06320 36 - 121 3.3e-22 GCN5-like protein 1 (GCN5L1)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 53
AccI GTMKAC 1 cut(s) 47
AccII CGCG 3 cut(s) 144, 176, 193
AciI CCGC 6 cut(s) 188, 197, 200, 218, 228, 287
AclWI GGATC 1 cut(s) 327
AcoI YGGCCR 1 cut(s) 288
AcyI GRCGYC 1 cut(s) 50
AfaI GTAC 1 cut(s) 5
AfiI CCNNNNNNNGG 1 cut(s) 77
AgsI TTSAA 1 cut(s) 236
AjnI CCWGG 2 cut(s) 76, 111
AluBI AGCT 3 cut(s) 139, 205, 268
AluI AGCT 3 cut(s) 139, 205, 268
Alw26I GTCTC 1 cut(s) 362
AlwI GGATC 1 cut(s) 327
AoxI GGCC 1 cut(s) 288
AspLEI GCGC 1 cut(s) 193
AspS9I GGNCC 2 cut(s) 109, 115
AsuHPI GGTGA 3 cut(s) 232, 298, 322
AvaII GGWCC 2 cut(s) 109, 115
BccI CCATC 2 cut(s) 202, 365
BceAI ACGGC 2 cut(s) 28, 241
BciT130I CCWGG 2 cut(s) 78, 113
BcoDI GTCTC 1 cut(s) 362
BisI GCNGC 4 cut(s) 189, 198, 229, 288
BlsI GCNGC 4 cut(s) 190, 199, 230, 289
Bme1390I CCNGG 2 cut(s) 78, 113
Bme18I GGWCC 2 cut(s) 109, 115
BmgT120I GGNCC 2 cut(s) 109, 115
BmiI GGNNCC 2 cut(s) 69, 75
BmrFI CCNGG 2 cut(s) 78, 113
BpuEI CTTGAG 2 cut(s) 119, 290
BsaHI GRCGYC 1 cut(s) 50
BsaXI ACNNNNNCTCC 2 cut(s) 113, 143
Bsc4I CCNNNNNNNGG 1 cut(s) 77
BseBI CCWGG 2 cut(s) 78, 113
BseLI CCNNNNNNNGG 1 cut(s) 77
Bsh1236I CGCG 3 cut(s) 144, 176, 193
BshFI GGCC 1 cut(s) 290
BsiSI CCGG 2 cut(s) 65, 71
BslI CCNNNNNNNGG 1 cut(s) 77
BsmAI GTCTC 1 cut(s) 362
BsnI GGCC 1 cut(s) 290
Bsp1407I TGTACA 1 cut(s) 3
Bsp143I GATC 2 cut(s) 177, 319
Bsp68I TCGCGA 1 cut(s) 144
BspACI CCGC 6 cut(s) 188, 197, 200, 218, 228, 287
BspANI GGCC 1 cut(s) 290
BspFNI CGCG 3 cut(s) 144, 176, 193
BspLI GGNNCC 2 cut(s) 69, 75
BspPI GGATC 1 cut(s) 327
BsrGI TGTACA 1 cut(s) 3
BssMI GATC 2 cut(s) 177, 319
BssNI GRCGYC 1 cut(s) 50
Bst2UI CCWGG 2 cut(s) 78, 113
Bst4CI ACNGT 1 cut(s) 334
Bst6I CTCTTC 1 cut(s) 24
BstACI GRCGYC 1 cut(s) 50
BstAUI TGTACA 1 cut(s) 3
BstC8I GCNNGC 2 cut(s) 41, 195
BstFNI CGCG 3 cut(s) 144, 176, 193
BstHHI GCGC 1 cut(s) 193
BstKTI GATC 2 cut(s) 180, 322
BstMAI GTCTC 1 cut(s) 362
BstMBI GATC 2 cut(s) 177, 319
BstMWI GCNNNNNNNGC 4 cut(s) 194, 197, 265, 287
BstNI CCWGG 2 cut(s) 78, 113
BstSCI CCNGG 2 cut(s) 76, 111
BstUI CGCG 3 cut(s) 144, 176, 193
BsuRI GGCC 1 cut(s) 290
BtsI GCAGTG 1 cut(s) 351
BtsIMutI CAGTG 2 cut(s) 329, 351
BtuMI TCGCGA 1 cut(s) 144
Cac8I GCNNGC 2 cut(s) 41, 195
CfoI GCGC 1 cut(s) 193
Cfr13I GGNCC 2 cut(s) 109, 115
CseI GACGC 2 cut(s) 101, 182
CsiI ACCWGGT 1 cut(s) 76
Csp6I GTAC 1 cut(s) 4
CviJI RGCY 7 cut(s) 70, 139, 205, 268, 281, 290, 328
CviKI_1 RGCY 7 cut(s) 70, 139, 205, 268, 281, 290, 328
CviQI GTAC 1 cut(s) 4
DpnI GATC 2 cut(s) 179, 321
DpnII GATC 2 cut(s) 177, 319
EaeI YGGCCR 1 cut(s) 288
Eam1104I CTCTTC 1 cut(s) 24
EarI CTCTTC 1 cut(s) 24
EciI GGCGGA 1 cut(s) 215
Eco47I GGWCC 2 cut(s) 109, 115
EcoRII CCWGG 2 cut(s) 76, 111
FaiI YATR 2 cut(s) 86, 347
FblI GTMKAC 1 cut(s) 47
Fnu4HI GCNGC 4 cut(s) 189, 198, 229, 288
Fsp4HI GCNGC 4 cut(s) 189, 198, 229, 288
GlaI GCGC 1 cut(s) 192
GluI GCNGC 4 cut(s) 189, 198, 229, 288
HaeIII GGCC 1 cut(s) 290
HapII CCGG 2 cut(s) 65, 71
HgaI GACGC 2 cut(s) 101, 182
HhaI GCGC 1 cut(s) 193
Hin1I GRCGYC 1 cut(s) 50
Hin6I GCGC 1 cut(s) 191
HinP1I GCGC 1 cut(s) 191
HincII GTYRAC 1 cut(s) 48
HindII GTYRAC 1 cut(s) 48
HindIII AAGCTT 1 cut(s) 137
HinfI GANTC 4 cut(s) 239, 261, 274, 303
HpaII CCGG 2 cut(s) 65, 71
HphI GGTGA 3 cut(s) 232, 298, 322
Hpy166II GTNNAC 4 cut(s) 6, 48, 223, 250
Hpy188I TCNGA 1 cut(s) 393
Hpy188III TCNNGA 3 cut(s) 143, 181, 236
Hpy8I GTNNAC 4 cut(s) 6, 48, 223, 250
Hpy99I CGWCG 3 cut(s) 49, 52, 55
HpyAV CCTTC 2 cut(s) 163, 178
HpyCH4III ACNGT 1 cut(s) 334
HpyCH4IV ACGT 1 cut(s) 50
HpyF10VI GCNNNNNNNGC 4 cut(s) 194, 197, 265, 287
HpySE526I ACGT 1 cut(s) 50
Hsp92I GRCGYC 1 cut(s) 50
HspAI GCGC 1 cut(s) 191
Kzo9I GATC 2 cut(s) 177, 319
LmnI GCTCC 3 cut(s) 67, 105, 202
LpnPI CCDG 6 cut(s) 63, 78, 84, 90, 98, 125
MabI ACCWGGT 1 cut(s) 76
MaeII ACGT 1 cut(s) 50
MaeIII GTNAC 1 cut(s) 334
MalI GATC 2 cut(s) 179, 321
MboI GATC 2 cut(s) 177, 319
MboII GAAGA 4 cut(s) 6, 11, 167, 178
MfeI CAATTG 1 cut(s) 104
MluCI AATT 2 cut(s) 104, 376
MlyI GAGTC 1 cut(s) 248
MnlI CCTC 3 cut(s) 82, 143, 208
MseI TTAA 1 cut(s) 375
MspI CCGG 2 cut(s) 65, 71
MspR9I CCNGG 2 cut(s) 78, 113
MunI CAATTG 1 cut(s) 104
MvaI CCWGG 2 cut(s) 78, 113
MvnI CGCG 3 cut(s) 144, 176, 193
MwoI GCNNNNNNNGC 4 cut(s) 194, 197, 265, 287
NdeII GATC 2 cut(s) 177, 319
NlaIV GGNNCC 2 cut(s) 69, 75
NmuCI GTSAC 1 cut(s) 334
NruI TCGCGA 1 cut(s) 144
PfeI GAWTC 3 cut(s) 261, 274, 303
PflFI GACNNNGTC 1 cut(s) 52
PfoI TCCNGGA 1 cut(s) 111
PkrI GCNGC 4 cut(s) 190, 199, 230, 289
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
Psp6I CCWGG 2 cut(s) 76, 111
PspGI CCWGG 2 cut(s) 76, 111
PspN4I GGNNCC 2 cut(s) 69, 75
PspPI GGNCC 2 cut(s) 109, 115
PsyI GACNNNGTC 1 cut(s) 52
RruI TCGCGA 1 cut(s) 144
RsaI GTAC 1 cut(s) 5
RsaNI GTAC 1 cut(s) 4
SalI GTCGAC 1 cut(s) 46
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 4 cut(s) 189, 198, 229, 288
Sau3AI GATC 2 cut(s) 177, 319
Sau96I GGNCC 2 cut(s) 109, 115
SchI GAGTC 1 cut(s) 248
ScrFI CCNGG 2 cut(s) 78, 113
SetI ASST 6 cut(s) 53, 82, 141, 207, 270, 366
SexAI ACCWGGT 1 cut(s) 76
SgrDI CGTCGACG 1 cut(s) 46
SinI GGWCC 2 cut(s) 109, 115
SmlI CTYRAG 2 cut(s) 134, 269
SmoI CTYRAG 2 cut(s) 134, 269
Sse9I AATT 2 cut(s) 104, 376
SsiI CCGC 6 cut(s) 188, 197, 200, 218, 228, 287
StyD4I CCNGG 2 cut(s) 76, 111
TaaI ACNGT 1 cut(s) 334
TaiI ACGT 1 cut(s) 53
TaqI TCGA 2 cut(s) 47, 264
TasI AATT 2 cut(s) 104, 376
TatI WGTACW 1 cut(s) 3
TauI GCSGC 4 cut(s) 191, 200, 231, 290
TfiI GAWTC 3 cut(s) 261, 274, 303
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 2 cut(s) 329, 358
TseFI GTSAC 1 cut(s) 334
Tsp45I GTSAC 1 cut(s) 334
TspDTI ATGAA 1 cut(s) 362
TspGWI ACGGA 1 cut(s) 332
TspRI CASTG 2 cut(s) 329, 358
Tth111I GACNNNGTC 1 cut(s) 52
VpaK11BI GGWCC 2 cut(s) 109, 115
XmiI GTMKAC 1 cut(s) 47
ZraI GACGTC 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.