Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0005264

Physical Location & Seq

Genomic Coordinates Forward (+)

5020235 .. 5024546

Gene Structure 4312 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1062400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2130 bp

ATGGATCAAAGGTGGAGAGTTTCTGGCGTTTCTCAGCACCGCCGGATTGCGGTGTTAGCGGTGGTGTTTCTTTTACTGTTTCATCAGAATCTGAGCCCGTGCTGGTCCCTTAACATTGAAGGTTTGGCGTTGTTGAGGTTCAGGGACAGGGTGGTGACAGACCCTTTTGGGGCTTTATCGAAATGGAACAACAACGGTGGAGAAGATGATCATTGTTCCTGGTTCGGGGTGGAGTGCTCGGATGGAAAAGTTGTCATCTTGAATTTGAGAGATCTTTGTCTTGGAGGAACGTTGGCTCCCGATTTAGGGAAGCTGGCTTACATAAAATCTATCATTTTACGCAATAATTCGTTTACTGGAAACATTCCGAAGGAGATTGCAGAGTTAATGGAGCTGGAGGTTTTGGACTTGGGATACAATAACTTTACTGGACCTTTCCCATCTGATTTCAGCAACAATCCGTCTCTTGCTACTCTTTTACTTGACAATAACAGATTTCTTGGTTCCATATCTTCTGAACTCCATCAATTGAAGATGCTTTTGGAATGCCGGGTAGATCAGGATCAACTAACAGATGCCGCTTTCTGTATTAGCAGATCTATCAGCTGGAGCACTGCCAAACCTGAAGATGCAGCTCACCGTAGGCTGCAGCAGGTGGCAGATGACAAAGCACCGTCCAGATTCAGAAGGGCTAAAGAAAATGAACGCGGTTCATTACCAACTTTGGCTTCTTCATCTTCCTCACCACCACTTCATCAGCCTTCTTTACCACCTTCACAGCCATTTTCAGTCTCATTGCCACCATCAACATTTTCATCTTCACTGTCATTAGATTCCCCTTTACCATCACCATCATCTTCTCCTTCTGCATCTCCCTCAAGTAGTTTTCCTGCTCCTTCACCCTCACCTTTGGTAGCGCCAACTCCCGCCTCTCCAGTCCCAAGAAATCCACCAACAACTATTTTTCCACCACCAGAGTCCAGTCCAGAACCAATTCCTTCCCCTTCTTTAAGTCCAAGTCAAGTGGCTCAAAGCAATTCCAATTCAAGGCATCGTGTAGTTATTATATGCGCAGGCGTTGTTGGGGGTTGTTTATTGATATCCCTTTCGGTTGTTGGCACTATTTTCGCCAGAAGCAGTAAAGTTGTTACTGTCAAGCCCTGGGCCACCGGGTTAAGTGGACAGTTGCAGAAAGCATTTGTCACAGGTGTACCAAAGCTGAACCGATCAGAACTTGAGGCAGCTTGTGAAGATTTCAGTAATGTAATTGGATCTCTCTCAGATGGCAACGTTTATAAGGGAACCCTTTCGAGTGGCGTTGAAATAGCTGTGACATCAATCACAATGGAATCCACTGCAGACTGGACCAAAAGTTTGGAGGCTCAGTTTAGAAAGAAGATAGAAACATTGTCCAAAGTAAACCACAAGAACTTTGTAAACCTTATCGGGTTTTGCGAAGAAGATAAACCATTCACAAGAATAATGGTTTTTGAGTATGCTCCAAATGGAACGCTTTTTGAGCATCTACACATAAAAGAAGCAGAGCACTTGGACTGGGGAATGCGACTACGAATAGCCATGGGCATGGCATACTGTCTAGAGTACATGCACCAACTGAAACCACCTATAGTTCAGAAGAACTTGCAATCATCCTCTGTATACCTGACCGAAGACTACGCAGCCAAAATATCCGATTTTACCTTCTTGAGTGAAGTGACAACAGCGAAGATGGGATCCGTTGACAAGAAGCTTTGCGATTCCATGTCCACAGATCCAGAAAGCAATGTCTACAGCTTCGGGGTAATATTGTTTGAAATGATCACCGGTAGGATTCCATACTCCGTGGAAAACGGTTCTCTTGAAGACTGGGCGTCACACTATCTGAAAGGAGATAAATCCTTGAGAGAAATAGTGGATCCTAGCCTAAACTCTTTCCAAGAAGAAGAGCTAAGTGAACTGTTTCGAGTGATAAAAGACTGCGTCCATCCTGACCCGAAACAAAGACCGACAATGACAGAGATCACGGGCAGGTTAAAGGTAATCACAGCCATGGGACCTGACGGAGCCACACCGAAACTATCACCTCTTTGGTGGGCAGAACTTGAAATTATGTCAACAGAAGGAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

LRRNT_2 LRR_8 Pkinase_Tyr

Protein Analysis

710

Amino Acids

77.78

Weight (kDa)

5.71

Isoelectric Point (pI)

58.38

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	40 - 80	4.8e-09	Leucine rich repeat N-terminal domain
LRR_8	PF13855	108 - 166	6.1e-07	Leucine rich repeat
PK_Tyr_Ser-Thr	PF07714	420 - 677	1.1e-27	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	421 - 675	7.2e-23	Protein kinase domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005264)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G58540 AT5G58540 AT5G58540
fragaria_vesca	FvH4_3g39310 FvH4_3g39310 FvH4_3g39310 FvH4_3g39310 FvH4_3g39310 FvH4_3g39310
malus_domestica	MD03G1062400.v1.1 MD11G1066000.v1.1
prunus_persica	Prupe.6G052400_v2.0.a1 Prupe.6G052400_v2.0.a1 Prupe.6G052400_v2.0.a1
pyrus_communis	pycom03g05000 pycom11g05590
rosa_chinensis	RchiOBHm_Chr5g0070741
rosa_laevigata	RLG00000036155
rosa_multiflora	Rmu_sc0002944.1_g000040 Rmu_sc0036080.1_g000001 Rmu_sc0041274.1_g000001
rosa_roxburghii	Rroxscaffold_1G00010450
rosa_rugosa	Rorug05G0408300 Rorug05G0408400
rosa_samantha	Rh5AG463700 Rh5BG481600 Rh5CG505900 Rh5DG494100
rosa_wichuraiana	Rw5G043160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	1296
AarI	CACCTGC	1 cut(s)	643
Acc16I	TGCGCA	1 cut(s)	1072
Acc36I	ACCTGC	2 cut(s)	643, 2019
AccI	GTMKAC	2 cut(s)	1659, 1788
AccII	CGCG	1 cut(s)	708
AciI	CCGC	6 cut(s)	40, 50, 59, 579, 708, 927
AclI	AACGTT	2 cut(s)	290, 1290
AclWI	GGATC	8 cut(s)	12, 570, 1279, 1728, 1741, 1766, 1910, 1923
AcsI	RAATTY	1 cut(s)	262
AcuI	CTGAAG	1 cut(s)	645
AcyI	GRCGYC	1 cut(s)	1871
AfaI	GTAC	2 cut(s)	1212, 1604
AfiI	CCNNNNNNNGG	6 cut(s)	49, 169, 225, 305, 306, 1047
AgeI	ACCGGT	1 cut(s)	1823
AgsI	TTSAA	8 cut(s)	119, 262, 532, 1047, 1322, 1814, 1862, 2105
AhdI	GACNNNNNGTC	1 cut(s)	1870
AjnI	CCWGG	2 cut(s)	218, 1160
AjuI	GAANNNNNNNTTGG	4 cut(s)	524, 556, 712, 744
AloI	GAACNNNNNNTCC	2 cut(s)	280, 312
Alw21I	GWGCWC	3 cut(s)	239, 614, 1548
Alw26I	GTCTC	2 cut(s)	468, 796
AlwI	GGATC	8 cut(s)	12, 570, 1279, 1728, 1741, 1766, 1910, 1923
AlwNI	CAGNNNCTG	1 cut(s)	91
AoxI	GGCC	1 cut(s)	1164
ApeKI	GCWGC	5 cut(s)	632, 646, 649, 1241, 1679
ApoI	RAATTY	1 cut(s)	262
ArsI	GACNNNNNNTTYG	2 cut(s)	1357, 1389
AsiGI	ACCGGT	1 cut(s)	1823
Asp700I	GAANNNNTTC	3 cut(s)	993, 1306, 1959
AspLEI	GCGC	2 cut(s)	919, 1073
AspS9I	GGNCC	5 cut(s)	105, 431, 1164, 1365, 2054
AsuC2I	CCSGG	2 cut(s)	551, 1171
AsuHPI	GGTGA	8 cut(s)	166, 629, 735, 840, 891, 897, 1813, 2073
AvaII	GGWCC	4 cut(s)	105, 431, 1365, 2054
BaeI	ACNNNNGTAYC	2 cut(s)	1194, 1227
BamHI	GGATCC	2 cut(s)	1733, 1915
BanII	GRGCYC	1 cut(s)	98
BarI	GAAGNNNNNNTAC	2 cut(s)	302, 334
BbsI	GAAGAC	2 cut(s)	1677, 1869
Bbv12I	GWGCWC	3 cut(s)	239, 614, 1548
BbvI	GCAGC	5 cut(s)	633, 644, 661, 1253, 1691
BccI	CCATC	9 cut(s)	236, 448, 531, 811, 853, 859, 1277, 1723, 1992
BcgI	CGANNNNNNTGC	2 cut(s)	1108, 1142
BciT130I	CCWGG	2 cut(s)	220, 1162
BciVI	GTATCC	1 cut(s)	407
BclI	TGATCA	2 cut(s)	208, 1818
BcnI	CCSGG	2 cut(s)	551, 1171
BcoDI	GTCTC	2 cut(s)	468, 796
BfaI	CTAG	2 cut(s)	1598, 1920
BfmI	CTRYAG	4 cut(s)	647, 1356, 1626, 1789
BfoI	RGCGCY	1 cut(s)	920
BfuAI	ACCTGC	2 cut(s)	643, 2019
BfuI	GTATCC	1 cut(s)	407
BglII	AGATCT	2 cut(s)	271, 596
BisI	GCNGC	6 cut(s)	579, 633, 647, 650, 1242, 1680
BlsI	GCNGC	6 cut(s)	580, 634, 648, 651, 1243, 1681
Bme1390I	CCNGG	4 cut(s)	220, 551, 1162, 1171
Bme18I	GGWCC	4 cut(s)	105, 431, 1365, 2054
BmeRI	GACNNNNNGTC	1 cut(s)	1870
BmgT120I	GGNCC	5 cut(s)	105, 431, 1164, 1365, 2054
BmiI	GGNNCC	8 cut(s)	107, 297, 505, 1303, 1735, 1917, 2055, 2065
BmrFI	CCNGG	4 cut(s)	220, 551, 1162, 1171
BmrI	ACTGGG	2 cut(s)	1564, 1876
BmsI	GCATC	6 cut(s)	525, 565, 619, 878, 1060, 1531
BmuI	ACTGGG	2 cut(s)	1564, 1876
BpiI	GAAGAC	2 cut(s)	1677, 1869
BpmI	CTGGAG	3 cut(s)	416, 628, 918
BpuEI	CTTGAG	4 cut(s)	862, 1256, 1726, 1921
BpuMI	CCSGG	2 cut(s)	551, 1171
BsaBI	GATNNNNATC	1 cut(s)	561
BsaHI	GRCGYC	1 cut(s)	1871
BsaJI	CCNNGG	5 cut(s)	1160, 1161, 1578, 1842, 2049
BsaWI	WCCGGW	1 cut(s)	1823
BsaXI	ACNNNNNCTCC	4 cut(s)	280, 310, 1370, 1400
Bsc4I	CCNNNNNNNGG	6 cut(s)	49, 169, 225, 305, 306, 1047
Bse118I	RCCGGY	1 cut(s)	1823
Bse1I	ACTGG	7 cut(s)	361, 433, 935, 981, 1367, 1559, 1871
Bse3DI	GCAATG	2 cut(s)	794, 1789
Bse8I	GATNNNNATC	1 cut(s)	561
BseBI	CCWGG	2 cut(s)	220, 1162
BseDI	CCNNGG	5 cut(s)	1160, 1161, 1578, 1842, 2049
BseGI	GGATG	3 cut(s)	247, 1649, 1984
BseJI	GATNNNNATC	1 cut(s)	561
BseLI	CCNNNNNNNGG	6 cut(s)	49, 169, 225, 305, 306, 1047
BseMI	GCAATG	2 cut(s)	794, 1789
BseMII	CTCAG	4 cut(s)	47, 83, 1293, 1397
BseNI	ACTGG	7 cut(s)	361, 433, 935, 981, 1367, 1559, 1871
BseXI	GCAGC	5 cut(s)	633, 644, 661, 1253, 1691
Bsh1236I	CGCG	1 cut(s)	708
BshFI	GGCC	1 cut(s)	1166
BshTI	ACCGGT	1 cut(s)	1823
BsiHKAI	GWGCWC	3 cut(s)	239, 614, 1548
BsiSI	CCGG	4 cut(s)	43, 550, 1170, 1824
BslFI	GGGAC	4 cut(s)	91, 158, 923, 2067
BslI	CCNNNNNNNGG	6 cut(s)	49, 169, 225, 305, 306, 1047
BsmAI	GTCTC	2 cut(s)	468, 796
BsmBI	CGTCTC	1 cut(s)	468
BsmFI	GGGAC	4 cut(s)	91, 158, 923, 2067
BsmI	GAATGC	2 cut(s)	551, 1566
BsnI	GGCC	1 cut(s)	1166
Bsp1286I	GDGCHC	4 cut(s)	98, 239, 614, 1548
Bsp19I	CCATGG	2 cut(s)	1578, 2049
BspACI	CCGC	6 cut(s)	40, 50, 59, 579, 708, 927
BspANI	GGCC	1 cut(s)	1166
BspCNI	CTCAG	4 cut(s)	46, 84, 1292, 1396
BspFNI	CGCG	1 cut(s)	708
BspLI	GGNNCC	8 cut(s)	107, 297, 505, 1303, 1735, 1917, 2055, 2065
BspMAI	CTGCAG	2 cut(s)	651, 1360
BspMI	ACCTGC	2 cut(s)	643, 2019
BspPI	GGATC	8 cut(s)	12, 570, 1279, 1728, 1741, 1766, 1910, 1923
BspQI	GCTCTTC	1 cut(s)	1938
BsrDI	GCAATG	2 cut(s)	794, 1789
BsrFI	RCCGGY	1 cut(s)	1823
BsrI	ACTGG	7 cut(s)	361, 433, 935, 981, 1367, 1559, 1871
BssAI	RCCGGY	1 cut(s)	1823
BssECI	CCNNGG	5 cut(s)	1160, 1161, 1578, 1842, 2049
BssNAI	GTATAC	1 cut(s)	1660
BssNI	GRCGYC	1 cut(s)	1871
BssT1I	CCWWGG	2 cut(s)	1578, 2049
Bst1107I	GTATAC	1 cut(s)	1660
Bst2UI	CCWGG	2 cut(s)	220, 1162
Bst6I	CTCTTC	1 cut(s)	1938
BstACI	GRCGYC	1 cut(s)	1871
BstC8I	GCNNGC	2 cut(s)	315, 1075
BstDEI	CTNAG	5 cut(s)	33, 92, 1279, 1383, 1949
BstDSI	CCRYGG	3 cut(s)	1578, 1842, 2049
BstF5I	GGATG	3 cut(s)	247, 1649, 1984
BstFNI	CGCG	1 cut(s)	708
BstH2I	RGCGCY	1 cut(s)	920
BstHHI	GCGC	2 cut(s)	919, 1073
BstMAI	GTCTC	2 cut(s)	468, 796
BstMWI	GCNNNNNNNGC	2 cut(s)	56, 1519
BstNI	CCWGG	2 cut(s)	220, 1162
BstNSI	RCATGY	1 cut(s)	1609
BstSCI	CCNGG	4 cut(s)	218, 549, 1160, 1169
BstSFI	CTRYAG	4 cut(s)	647, 1356, 1626, 1789
BstUI	CGCG	1 cut(s)	708
BstV1I	GCAGC	5 cut(s)	633, 644, 661, 1253, 1691
BstV2I	GAAGAC	2 cut(s)	1677, 1869
BstX2I	RGATCY	6 cut(s)	271, 596, 1271, 1733, 1771, 1915
BstXI	CCANNNNNNTGG	2 cut(s)	1375, 1585
BstYI	RGATCY	6 cut(s)	271, 596, 1271, 1733, 1771, 1915
BstZ17I	GTATAC	1 cut(s)	1660
BsuI	GTATCC	1 cut(s)	407
BsuRI	GGCC	1 cut(s)	1166
BtgI	CCRYGG	3 cut(s)	1578, 1842, 2049
BtsCI	GGATG	3 cut(s)	247, 1649, 1984
BtsI	GCAGTG	2 cut(s)	612, 1353
BtsIMutI	CAGTG	3 cut(s)	612, 821, 1353
BveI	ACCTGC	2 cut(s)	643, 2019
Cac8I	GCNNGC	2 cut(s)	315, 1075
CaiI	CAGNNNCTG	1 cut(s)	91
CfoI	GCGC	2 cut(s)	919, 1073
Cfr10I	RCCGGY	1 cut(s)	1823
Cfr13I	GGNCC	5 cut(s)	105, 431, 1164, 1365, 2054
CseI	GACGC	2 cut(s)	1860, 1969
Csp6I	GTAC	2 cut(s)	1211, 1603
CspAI	ACCGGT	1 cut(s)	1823
CspCI	CAANNNNNGTGG	4 cut(s)	178, 213, 1005, 1040
CviAII	CATG	5 cut(s)	1579, 1585, 1606, 1762, 2050
CviQI	GTAC	2 cut(s)	1211, 1603
DdeI	CTNAG	5 cut(s)	33, 92, 1279, 1383, 1949
DriI	GACNNNNNGTC	1 cut(s)	1870
Eam1104I	CTCTTC	1 cut(s)	1938
Eam1105I	GACNNNNNGTC	1 cut(s)	1870
EarI	CTCTTC	1 cut(s)	1938
Eco130I	CCWWGG	2 cut(s)	1578, 2049
Eco24I	GRGCYC	1 cut(s)	98
Eco32I	GATATC	1 cut(s)	1101
Eco47I	GGWCC	4 cut(s)	105, 431, 1365, 2054
Eco57I	CTGAAG	1 cut(s)	645
EcoO109I	RGGNCCY	1 cut(s)	2054
EcoRII	CCWGG	2 cut(s)	218, 1160
EcoRV	GATATC	1 cut(s)	1101
EcoT14I	CCWWGG	2 cut(s)	1578, 2049
EcoT38I	GRGCYC	1 cut(s)	98
ErhI	CCWWGG	2 cut(s)	1578, 2049
Esp3I	CGTCTC	1 cut(s)	468
FaeI	CATG	5 cut(s)	1582, 1588, 1609, 1765, 2053
FaqI	GGGAC	4 cut(s)	91, 158, 923, 2067
FatI	CATG	5 cut(s)	1578, 1584, 1605, 1761, 2049
FauI	CCCGC	1 cut(s)	934
FbaI	TGATCA	2 cut(s)	208, 1818
FblI	GTMKAC	2 cut(s)	1659, 1788
Fnu4HI	GCNGC	6 cut(s)	579, 633, 647, 650, 1242, 1680
FokI	GGATG	3 cut(s)	254, 1636, 1971
FriOI	GRGCYC	1 cut(s)	98
Fsp4HI	GCNGC	6 cut(s)	579, 633, 647, 650, 1242, 1680
FspBI	CTAG	2 cut(s)	1598, 1920
FspI	TGCGCA	1 cut(s)	1072
GlaI	GCGC	2 cut(s)	918, 1072
GluI	GCNGC	6 cut(s)	579, 633, 647, 650, 1242, 1680
GsuI	CTGGAG	3 cut(s)	416, 628, 918
HaeII	RGCGCY	1 cut(s)	920
HaeIII	GGCC	1 cut(s)	1166
HapII	CCGG	4 cut(s)	43, 550, 1170, 1824
HgaI	GACGC	2 cut(s)	1860, 1969
HhaI	GCGC	2 cut(s)	919, 1073
Hin1I	GRCGYC	1 cut(s)	1871
Hin1II	CATG	5 cut(s)	1582, 1588, 1609, 1765, 2053
Hin6I	GCGC	2 cut(s)	917, 1071
HinP1I	GCGC	2 cut(s)	917, 1071
HincII	GTYRAC	2 cut(s)	1741, 2115
HindII	GTYRAC	2 cut(s)	1741, 2115
HindIII	AAGCTT	1 cut(s)	1748
HinfI	GANTC	7 cut(s)	88, 681, 833, 977, 1349, 1757, 1831
HpaII	CCGG	4 cut(s)	43, 550, 1170, 1824
HphI	GGTGA	8 cut(s)	166, 629, 735, 840, 891, 897, 1813, 2073
HpyCH4IV	ACGT	2 cut(s)	290, 1290
HpyCH4V	TGCA	8 cut(s)	380, 632, 649, 869, 1189, 1358, 1609, 1645
HpyF10VI	GCNNNNNNNGC	2 cut(s)	56, 1519
HpyF3I	CTNAG	5 cut(s)	33, 92, 1279, 1383, 1949
HpySE526I	ACGT	2 cut(s)	290, 1290
Hsp92I	GRCGYC	1 cut(s)	1871
Hsp92II	CATG	5 cut(s)	1582, 1588, 1609, 1765, 2053
HspAI	GCGC	2 cut(s)	917, 1071
Ksp22I	TGATCA	2 cut(s)	208, 1818
LguI	GCTCTTC	1 cut(s)	1938
LmnI	GCTCC	6 cut(s)	301, 391, 609, 898, 1504, 2063
Lsp1109I	GCAGC	5 cut(s)	633, 644, 661, 1253, 1691
LweI	GCATC	6 cut(s)	525, 565, 619, 878, 1060, 1531
MaeI	CTAG	2 cut(s)	1598, 1920
MaeII	ACGT	2 cut(s)	290, 1290
MaeIII	GTNAC	6 cut(s)	154, 1147, 1201, 1330, 1714, 1872
MfeI	CAATTG	1 cut(s)	527
MflI	RGATCY	6 cut(s)	271, 596, 1271, 1733, 1771, 1915
MhlI	GDGCHC	4 cut(s)	98, 239, 614, 1548
MluCI	AATT	8 cut(s)	262, 346, 527, 993, 1036, 1042, 1266, 2106
MlyI	GAGTC	1 cut(s)	986
MroXI	GAANNNNTTC	3 cut(s)	993, 1306, 1959
MseI	TTAA	5 cut(s)	111, 386, 1010, 1175, 2033
MslI	CAYNNNNRTG	2 cut(s)	1583, 2048
MspA1I	CMGCKG	1 cut(s)	606
MspI	CCGG	4 cut(s)	43, 550, 1170, 1824
MspR9I	CCNGG	4 cut(s)	220, 551, 1162, 1171
MunI	CAATTG	1 cut(s)	527
Mva1269I	GAATGC	2 cut(s)	551, 1566
MvaI	CCWGG	2 cut(s)	220, 1162
MvnI	CGCG	1 cut(s)	708
MwoI	GCNNNNNNNGC	2 cut(s)	56, 1519
NciI	CCSGG	2 cut(s)	551, 1171
NcoI	CCATGG	2 cut(s)	1578, 2049
NlaIII	CATG	5 cut(s)	1582, 1588, 1609, 1765, 2053
NlaIV	GGNNCC	8 cut(s)	107, 297, 505, 1303, 1735, 1917, 2055, 2065
NmuCI	GTSAC	5 cut(s)	154, 1201, 1330, 1714, 1872
NsbI	TGCGCA	1 cut(s)	1072
NspI	RCATGY	1 cut(s)	1609
PaqCI	CACCTGC	1 cut(s)	643
PasI	CCCWGGG	1 cut(s)	1161
PciSI	GCTCTTC	1 cut(s)	1938
PcsI	WCGNNNNNNNCGW	1 cut(s)	1452
PctI	GAATGC	2 cut(s)	551, 1566
PdmI	GAANNNNTTC	3 cut(s)	993, 1306, 1959
PfeI	GAWTC	6 cut(s)	88, 681, 833, 1349, 1757, 1831
PflFI	GACNNNGTC	1 cut(s)	1979
PinAI	ACCGGT	1 cut(s)	1823
PkrI	GCNGC	6 cut(s)	580, 634, 648, 651, 1243, 1681
PleI	GAGTC	1 cut(s)	985
PpsI	GAGTC	1 cut(s)	985
PpuMI	RGGWCCY	1 cut(s)	2054
PsiI	TTATAA	1 cut(s)	1296
Psp1406I	AACGTT	2 cut(s)	290, 1290
Psp5II	RGGWCCY	1 cut(s)	2054
Psp6I	CCWGG	2 cut(s)	218, 1160
PspGI	CCWGG	2 cut(s)	218, 1160
PspN4I	GGNNCC	8 cut(s)	107, 297, 505, 1303, 1735, 1917, 2055, 2065
PspPI	GGNCC	5 cut(s)	105, 431, 1164, 1365, 2054
PspPPI	RGGWCCY	1 cut(s)	2054
PstI	CTGCAG	2 cut(s)	651, 1360
PstNI	CAGNNNCTG	1 cut(s)	91
PsuI	RGATCY	6 cut(s)	271, 596, 1271, 1733, 1771, 1915
PsyI	GACNNNGTC	1 cut(s)	1979
PvuII	CAGCTG	1 cut(s)	606
RsaI	GTAC	2 cut(s)	1212, 1604
RsaNI	GTAC	2 cut(s)	1211, 1603
RseI	CAYNNNNRTG	2 cut(s)	1583, 2048
SapI	GCTCTTC	1 cut(s)	1938
SaqAI	TTAA	5 cut(s)	111, 386, 1010, 1175, 2033
SatI	GCNGC	6 cut(s)	579, 633, 647, 650, 1242, 1680
Sau96I	GGNCC	5 cut(s)	105, 431, 1164, 1365, 2054
SchI	GAGTC	1 cut(s)	986
ScrFI	CCNGG	4 cut(s)	220, 551, 1162, 1171
SduI	GDGCHC	4 cut(s)	98, 239, 614, 1548
SfaNI	GCATC	6 cut(s)	525, 565, 619, 878, 1060, 1531
SfcI	CTRYAG	4 cut(s)	647, 1356, 1626, 1789
SinI	GGWCC	4 cut(s)	105, 431, 1365, 2054
SmiMI	CAYNNNNRTG	2 cut(s)	1583, 2048
SmlI	CTYRAG	4 cut(s)	877, 1235, 1705, 1900
SmoI	CTYRAG	4 cut(s)	877, 1235, 1705, 1900
Sse9I	AATT	8 cut(s)	262, 346, 527, 993, 1036, 1042, 1266, 2106
SsiI	CCGC	6 cut(s)	40, 50, 59, 579, 708, 927
SspI	AATATT	1 cut(s)	1806
SspMI	CTAG	2 cut(s)	1598, 1920
StyD4I	CCNGG	4 cut(s)	218, 549, 1160, 1169
StyI	CCWWGG	2 cut(s)	1578, 2049
TaiI	ACGT	2 cut(s)	293, 1293
TaqI	TCGA	3 cut(s)	179, 1310, 1963
TaqII	GACCGA	2 cut(s)	1682, 2020
TasI	AATT	8 cut(s)	262, 346, 527, 993, 1036, 1042, 1266, 2106
TatI	WGTACW	1 cut(s)	1602
TauI	GCSGC	1 cut(s)	581
TfiI	GAWTC	6 cut(s)	88, 681, 833, 1349, 1757, 1831
Tru1I	TTAA	5 cut(s)	111, 386, 1010, 1175, 2033
Tru9I	TTAA	5 cut(s)	111, 386, 1010, 1175, 2033
TscAI	CASTG	3 cut(s)	619, 828, 1360
TseFI	GTSAC	5 cut(s)	154, 1201, 1330, 1714, 1872
TseI	GCWGC	5 cut(s)	632, 646, 649, 1241, 1679
Tsp45I	GTSAC	5 cut(s)	154, 1201, 1330, 1714, 1872
TspDTI	ATGAA	6 cut(s)	71, 702, 717, 723, 743, 804
TspGWI	ACGGA	4 cut(s)	450, 1726, 1831, 2076
TspRI	CASTG	3 cut(s)	619, 828, 1360
Tth111I	GACNNNGTC	1 cut(s)	1979
VpaK11BI	GGWCC	4 cut(s)	105, 431, 1365, 2054
XapI	RAATTY	1 cut(s)	262
XbaI	TCTAGA	1 cut(s)	1597
XceI	RCATGY	1 cut(s)	1609
XmiI	GTMKAC	2 cut(s)	1659, 1788
XmnI	GAANNNNTTC	3 cut(s)	993, 1306, 1959
XspI	CTAG	2 cut(s)	1598, 1920

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.