MD03G1086600.v1.1

DnaJ homolog subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0011368
Physical Location & Seq
Reverse (-)
7076225 .. 7079585
3361 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1086600.v1.1.491

Sequence Viewer

Length: 798 bp
ATGCATGGATTGGCTCTCCTGCCTCTAACTCCACACACCCTCTGTTTCTTCAACCTCCCTCCTTCTTCTTCTTCTTCTTCTTCTTCGAGATCTCTGCTTTTTCTGCAAACCCGACCCGAATTTTCGAAACCCGTAACCAGCTTCTCGAATTTGAACCAGAAACGGCGGCGTTTTGCGACCGTTTTGAGAGCGAGTCGCAGAGAGTCTCCTTACCAGGTTCTGGGCGTGTCTCCTTCTGCAACTGATGTCGAGATCAAAAGGGCTTATCGCAAACTTGCTCTTAAGTATCACCCCGATGTTAACAAAGAGGCAAATGCACAGGAGAAATTTATGCGGATTAAGCATGCCTACAATACATTGCTGAGTTCTAAATCTCGGGGAAAATACGACTCTAATTTCGGATCTGACTATTCCTATTCCTCTGCTCAAAAAAACCAGAGCAAGAAGTCTCAAGATGAAGAGGAGTTTTACGGATTTGAGGACTTCTTTAAAGACATTCAAGAAGAATTCCAGAACTGGGAAGCGAGTGCTTCGTCACAAGGAAAGCCAAAGAGTCTATGGGAGGAACTGGGGGAGATTGGAGAAGAACTGGTGGAATTTCTTGAGAAAGAACTCAACATAACAGATCCAGAAGACGGAGTAAATAACAGTGATGAAGGCAATCCTTTCAGAAGCTCCGGGGAACAGAGAACAGGAAATGTTGGTCAAAACGAAACTGGTAAGGAGAGCAGCATAGAGGATAACATTGATGAGATAGAAGCTACTCTTGCTCGGTTAAAAAAGGAATTGGGATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

266

Amino Acids

30.01

Weight (kDa)

5.47

Isoelectric Point (pI)

65.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 70 - 130 3.5e-23 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011368)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G59610 AT5G59610 AT5G59610 AT5G59610 AT5G59610
fragaria_vesca FvH4_3g36901
malus_domestica MD03G1086600.v1.1 MD11G1095700.v1.1
prunus_persica Prupe.6G071200_v2.0.a1 Prupe.6G071200_v2.0.a1
pyrus_communis pycom05g24500
rosa_chinensis RchiOBHm_Chr5g0066291
rosa_laevigata RLG00000035848
rosa_multiflora Rmu_sc0006887.1_g000014
rosa_rugosa Rorug05G0376900
rosa_samantha Rh5AG435500 Rh5BG451700 Rh5CG474000 Rh5DG466600
rosa_wichuraiana Rw5G040780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 220
AciI CCGC 2 cut(s) 166, 334
AclWI GGATC 2 cut(s) 409, 620
AcsI RAATTY 5 cut(s) 119, 148, 326, 506, 596
AfiI CCNNNNNNNGG 3 cut(s) 220, 517, 635
AflII CTTAAG 1 cut(s) 281
AgsI TTSAA 3 cut(s) 52, 154, 500
AjnI CCWGG 1 cut(s) 213
AluBI AGCT 3 cut(s) 141, 675, 761
AluI AGCT 3 cut(s) 141, 675, 761
Alw26I GTCTC 3 cut(s) 210, 234, 453
AlwI GGATC 2 cut(s) 409, 620
AlwNI CAGNNNCTG 1 cut(s) 220
Ama87I CYCGRG 1 cut(s) 375
ApeKI GCWGC 1 cut(s) 729
ApoI RAATTY 5 cut(s) 119, 148, 326, 506, 596
ArsI GACNNNNNNTTYG 2 cut(s) 380, 412
AsuC2I CCSGG 1 cut(s) 679
AsuHPI GGTGA 1 cut(s) 281
AsuII TTCGAA 1 cut(s) 125
AvaI CYCGRG 1 cut(s) 375
BbsI GAAGAC 1 cut(s) 639
BbvI GCAGC 1 cut(s) 741
BceAI ACGGC 1 cut(s) 179
BcgI CGANNNNNNTGC 2 cut(s) 76, 110
BciT130I CCWGG 1 cut(s) 215
BcnI CCSGG 1 cut(s) 679
BcoDI GTCTC 3 cut(s) 210, 234, 453
BfrI CTTAAG 1 cut(s) 281
BglII AGATCT 1 cut(s) 89
BisI GCNGC 2 cut(s) 167, 730
BlsI GCNGC 2 cut(s) 168, 731
Bme1390I CCNGG 2 cut(s) 215, 679
BmeT110I CYCGRG 1 cut(s) 375
BmrFI CCNGG 2 cut(s) 215, 679
BmrI ACTGGG 2 cut(s) 526, 578
BmuI ACTGGG 2 cut(s) 526, 578
BpiI GAAGAC 1 cut(s) 639
Bpu14I TTCGAA 1 cut(s) 125
BpuEI CTTGAG 2 cut(s) 435, 623
BpuMI CCSGG 1 cut(s) 679
BsaJI CCNNGG 1 cut(s) 678
Bsc4I CCNNNNNNNGG 3 cut(s) 220, 517, 635
Bse1I ACTGG 4 cut(s) 521, 573, 594, 721
Bse3DI GCAATG 1 cut(s) 356
BseBI CCWGG 1 cut(s) 215
BseDI CCNNGG 1 cut(s) 678
BseLI CCNNNNNNNGG 3 cut(s) 220, 517, 635
BseMI GCAATG 1 cut(s) 356
BseMII CTCAG 1 cut(s) 353
BseNI ACTGG 4 cut(s) 521, 573, 594, 721
BseRI GAGGAG 1 cut(s) 476
BseXI GCAGC 1 cut(s) 741
Bsh1285I CGRYCG 1 cut(s) 180
BsiEI CGRYCG 1 cut(s) 180
BsiHKCI CYCGRG 1 cut(s) 375
BsiSI CCGG 1 cut(s) 678
BslI CCNNNNNNNGG 3 cut(s) 220, 517, 635
BsmAI GTCTC 3 cut(s) 210, 234, 453
BsoBI CYCGRG 1 cut(s) 375
Bsp119I TTCGAA 1 cut(s) 125
Bsp143I GATC 4 cut(s) 89, 252, 401, 625
BspACI CCGC 2 cut(s) 166, 334
BspCNI CTCAG 1 cut(s) 354
BspPI GGATC 2 cut(s) 409, 620
BspT104I TTCGAA 1 cut(s) 125
BspTI CTTAAG 1 cut(s) 281
BsrDI GCAATG 1 cut(s) 356
BsrI ACTGG 4 cut(s) 521, 573, 594, 721
BssECI CCNNGG 1 cut(s) 678
BssMI GATC 4 cut(s) 89, 252, 401, 625
Bst2UI CCWGG 1 cut(s) 215
Bst4CI ACNGT 2 cut(s) 181, 650
Bst6I CTCTTC 1 cut(s) 453
BstAFI CTTAAG 1 cut(s) 281
BstBI TTCGAA 1 cut(s) 125
BstC8I GCNNGC 1 cut(s) 345
BstDEI CTNAG 1 cut(s) 362
BstKTI GATC 4 cut(s) 92, 255, 404, 628
BstMAI GTCTC 3 cut(s) 210, 234, 453
BstMBI GATC 4 cut(s) 89, 252, 401, 625
BstMCI CGRYCG 1 cut(s) 180
BstMWI GCNNNNNNNGC 3 cut(s) 103, 340, 767
BstNI CCWGG 1 cut(s) 215
BstNSI RCATGY 1 cut(s) 347
BstSCI CCNGG 2 cut(s) 213, 677
BstV1I GCAGC 1 cut(s) 741
BstV2I GAAGAC 1 cut(s) 639
BstX2I RGATCY 3 cut(s) 89, 401, 625
BstYI RGATCY 3 cut(s) 89, 401, 625
BtsIMutI CAGTG 1 cut(s) 655
Cac8I GCNNGC 1 cut(s) 345
CaiI CAGNNNCTG 1 cut(s) 220
CsiI ACCWGGT 1 cut(s) 213
CviAII CATG 2 cut(s) 5, 344
CviJI RGCY 6 cut(s) 14, 141, 263, 547, 675, 761
CviKI_1 RGCY 6 cut(s) 14, 141, 263, 547, 675, 761
DdeI CTNAG 1 cut(s) 362
DpnI GATC 4 cut(s) 91, 254, 403, 627
DpnII GATC 4 cut(s) 89, 252, 401, 625
DraI TTTAAA 1 cut(s) 490
Eam1104I CTCTTC 1 cut(s) 453
EarI CTCTTC 1 cut(s) 453
Eco88I CYCGRG 1 cut(s) 375
EcoRI GAATTC 1 cut(s) 506
EcoRII CCWGG 1 cut(s) 213
EcoT22I ATGCAT 1 cut(s) 6
FaeI CATG 2 cut(s) 8, 347
FaiI YATR 6 cut(s) 6, 332, 345, 559, 620, 734
FalI AAGNNNNNCTT 2 cut(s) 750, 782
FatI CATG 2 cut(s) 4, 343
Fnu4HI GCNGC 2 cut(s) 167, 730
Fsp4HI GCNGC 2 cut(s) 167, 730
GluI GCNGC 2 cut(s) 167, 730
HapII CCGG 1 cut(s) 678
Hin1II CATG 2 cut(s) 8, 347
HincII GTYRAC 1 cut(s) 301
HindII GTYRAC 1 cut(s) 301
HinfI GANTC 4 cut(s) 193, 203, 389, 553
HpaI GTTAAC 1 cut(s) 301
HpaII CCGG 1 cut(s) 678
HphI GGTGA 1 cut(s) 281
Hpy166II GTNNAC 1 cut(s) 301
Hpy188I TCNGA 3 cut(s) 401, 406, 671
Hpy188III TCNNGA 8 cut(s) 87, 145, 250, 452, 500, 511, 602, 629
Hpy8I GTNNAC 1 cut(s) 301
HpyAV CCTTC 3 cut(s) 72, 243, 650
HpyCH4III ACNGT 2 cut(s) 181, 650
HpyCH4V TGCA 4 cut(s) 4, 106, 239, 317
HpyF10VI GCNNNNNNNGC 3 cut(s) 103, 340, 767
HpyF3I CTNAG 1 cut(s) 362
Hsp92II CATG 2 cut(s) 8, 347
KspAI GTTAAC 1 cut(s) 301
Kzo9I GATC 4 cut(s) 89, 252, 401, 625
LmnI GCTCC 1 cut(s) 680
Lsp1109I GCAGC 1 cut(s) 741
MabI ACCWGGT 1 cut(s) 213
MaeIII GTNAC 2 cut(s) 133, 534
MalI GATC 4 cut(s) 91, 254, 403, 627
MboI GATC 4 cut(s) 89, 252, 401, 625
MflI RGATCY 3 cut(s) 89, 401, 625
MluCI AATT 7 cut(s) 119, 148, 326, 394, 506, 596, 785
MlyI GAGTC 4 cut(s) 202, 212, 383, 562
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 5 cut(s) 282, 300, 339, 489, 776
MslI CAYNNNNRTG 1 cut(s) 294
MspCI CTTAAG 1 cut(s) 281
MspI CCGG 1 cut(s) 678
MspR9I CCNGG 2 cut(s) 215, 679
MvaI CCWGG 1 cut(s) 215
MwoI GCNNNNNNNGC 3 cut(s) 103, 340, 767
NciI CCSGG 1 cut(s) 679
NdeII GATC 4 cut(s) 89, 252, 401, 625
NlaIII CATG 2 cut(s) 8, 347
NmuCI GTSAC 1 cut(s) 534
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 347
NspV TTCGAA 1 cut(s) 125
PaeI GCATGC 1 cut(s) 347
PflMI CCANNNNNTGG 1 cut(s) 220
PkrI GCNGC 2 cut(s) 168, 731
PleI GAGTC 4 cut(s) 201, 211, 383, 561
PpsI GAGTC 4 cut(s) 201, 211, 383, 561
Psp6I CCWGG 1 cut(s) 213
PspGI CCWGG 1 cut(s) 213
PstNI CAGNNNCTG 1 cut(s) 220
PsuI RGATCY 3 cut(s) 89, 401, 625
RseI CAYNNNNRTG 1 cut(s) 294
SaqAI TTAA 5 cut(s) 282, 300, 339, 489, 776
SatI GCNGC 2 cut(s) 167, 730
Sau3AI GATC 4 cut(s) 89, 252, 401, 625
SchI GAGTC 4 cut(s) 202, 212, 383, 562
ScrFI CCNGG 2 cut(s) 215, 679
SetI ASST 5 cut(s) 57, 143, 219, 677, 763
SexAI ACCWGGT 1 cut(s) 213
SfuI TTCGAA 1 cut(s) 125
SmiMI CAYNNNNRTG 1 cut(s) 294
SmlI CTYRAG 3 cut(s) 281, 450, 602
SmoI CTYRAG 3 cut(s) 281, 450, 602
SphI GCATGC 1 cut(s) 347
Sse9I AATT 7 cut(s) 119, 148, 326, 394, 506, 596, 785
SsiI CCGC 2 cut(s) 166, 334
StyD4I CCNGG 2 cut(s) 213, 677
TaaI ACNGT 2 cut(s) 181, 650
TaqI TCGA 4 cut(s) 86, 125, 146, 249
TasI AATT 7 cut(s) 119, 148, 326, 394, 506, 596, 785
TauI GCSGC 1 cut(s) 169
Tru1I TTAA 5 cut(s) 282, 300, 339, 489, 776
Tru9I TTAA 5 cut(s) 282, 300, 339, 489, 776
TscAI CASTG 1 cut(s) 655
TseFI GTSAC 1 cut(s) 534
TseI GCWGC 1 cut(s) 729
Tsp45I GTSAC 1 cut(s) 534
TspDTI ATGAA 2 cut(s) 471, 669
TspGWI ACGGA 2 cut(s) 486, 651
TspRI CASTG 1 cut(s) 655
Van91I CCANNNNNTGG 1 cut(s) 220
Vha464I CTTAAG 1 cut(s) 281
XapI RAATTY 5 cut(s) 119, 148, 326, 506, 596
XceI RCATGY 1 cut(s) 347
XcmI CCANNNNNNNNNTGG 1 cut(s) 555
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.