Rw5G040780

DnaJ homolog subfamily

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0011368
Physical Location & Seq
Forward (+)
72030191 .. 72032967
2777 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G040780.1

Sequence Viewer

Length: 843 bp
ATGCATGGTTTTCTGGCTCTACTGCCTCTAACACCACCACAGCTCTATTTCTTCAACCTCTCTTCTTCTTCTTCTTCTTCTTCAAGATCTCTTCTTTACCTGCAAATCAGACCCAAATCTTCAACACCCATTTTGGGATACCCCTCAAAAACCAACCTGAATCTCGTCGTGTTCTCCTCCCACTCTTCGAGTTTCTCGAATTTCAGTAGCAAACGACGACGTTTTGTTACGAAAGCGAGTCGCAGAGAGTCCCCGTACCAAGTTTTGGGGGTGTCTCCTTCTGCTACTGATGTCGAGATCAAGAAGGCGTATCGGAAACTTGCCCTCAAGTATCACCCAGATGTTAATAAAGAGCCAAATGCGCAGGAGAAATTTATGAGGATTAAACATGCCTATAGTACATTGCTGAATTCAAAATCTCGGGGGAATTATGACTCTAATTATGGATCTGACTATTCCTATTCTGGTTCTCAAAGGAGCCAAAGCAAGAAGGCTCAAGATGAAGAAGAGTTTTATGGATTCGGTAATTTTGTGAGGGATGTTCAAATAACAATAGAGGACTTCTTTAAAGATATTCAAGCAGAATTCCAGAACTGGGAAGAAAGTGCTTCTACACAAGGAAAGCCAAGGAGTCTGTGGGAGGAATTGTCGGAAATTGGAGAAGAATTTGTAGAATTTCTCGAGAAAGAACTCAACATAACGGATCCAGAGGTTGGATCAGATAACATCAATGAAGGGCCTGAGAAGGGTCCATTCAGAAATTCTGGGAAAGAGACAACGGAAAGTGCAAGTAAAAAGGAGCCAGTATGGGGGCTAGTATTGAAGAGAATATTGATGAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DnaJ

Protein Analysis

280

Amino Acids

31.91

Weight (kDa)

8.8

Isoelectric Point (pI)

64.12

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DnaJ PF00226 85 - 145 1.7e-24 DnaJ domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011368)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G59610 AT5G59610 AT5G59610 AT5G59610 AT5G59610
fragaria_vesca FvH4_3g36901
malus_domestica MD03G1086600.v1.1 MD11G1095700.v1.1
prunus_persica Prupe.6G071200_v2.0.a1 Prupe.6G071200_v2.0.a1
pyrus_communis pycom05g24500
rosa_chinensis RchiOBHm_Chr5g0066291
rosa_laevigata RLG00000035848
rosa_multiflora Rmu_sc0006887.1_g000014
rosa_rugosa Rorug05G0376900
rosa_samantha Rh5AG435500 Rh5BG451700 Rh5CG474000 Rh5DG466600
rosa_wichuraiana Rw5G040780

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 363
Acc36I ACCTGC 1 cut(s) 108
AccB7I CCANNNNNTGG 2 cut(s) 265, 713
AclWI GGATC 4 cut(s) 454, 698, 711, 724
AcsI RAATTY 7 cut(s) 199, 371, 409, 584, 665, 674, 760
AfaI GTAC 2 cut(s) 257, 400
AfiI CCNNNNNNNGG 6 cut(s) 134, 265, 595, 713, 746, 809
AgsI TTSAA 7 cut(s) 55, 84, 123, 414, 545, 578, 823
AluBI AGCT 1 cut(s) 43
AluI AGCT 1 cut(s) 43
Alw26I GTCTC 2 cut(s) 279, 767
AlwI GGATC 4 cut(s) 454, 698, 711, 724
Ama87I CYCGRG 2 cut(s) 420, 680
AoxI GGCC 1 cut(s) 737
ApoI RAATTY 7 cut(s) 199, 371, 409, 584, 665, 674, 760
AspLEI GCGC 1 cut(s) 364
AspS9I GGNCC 2 cut(s) 737, 749
AsuHPI GGTGA 1 cut(s) 326
AvaI CYCGRG 2 cut(s) 420, 680
AvaII GGWCC 1 cut(s) 749
BamHI GGATCC 1 cut(s) 703
BciVI GTATCC 1 cut(s) 131
BcoDI GTCTC 2 cut(s) 279, 767
BfaI CTAG 1 cut(s) 815
BfmI CTRYAG 1 cut(s) 394
BfuAI ACCTGC 1 cut(s) 108
BfuI GTATCC 1 cut(s) 131
BglII AGATCT 1 cut(s) 86
Bme18I GGWCC 1 cut(s) 749
BmeT110I CYCGRG 2 cut(s) 420, 680
BmgT120I GGNCC 2 cut(s) 737, 749
BmiI GGNNCC 4 cut(s) 479, 705, 750, 801
BmrI ACTGGG 1 cut(s) 604
BmuI ACTGGG 1 cut(s) 604
BpuEI CTTGAG 2 cut(s) 311, 480
BsaJI CCNNGG 1 cut(s) 626
BsaXI ACNNNNNCTCC 2 cut(s) 632, 662
Bsc4I CCNNNNNNNGG 6 cut(s) 134, 265, 595, 713, 746, 809
Bse1I ACTGG 2 cut(s) 599, 803
Bse3DI GCAATG 1 cut(s) 401
BseDI CCNNGG 1 cut(s) 626
BseGI GGATG 1 cut(s) 544
BseLI CCNNNNNNNGG 6 cut(s) 134, 265, 595, 713, 746, 809
BseMI GCAATG 1 cut(s) 401
BseMII CTCAG 1 cut(s) 732
BseNI ACTGG 2 cut(s) 599, 803
BseRI GAGGAG 1 cut(s) 166
BshFI GGCC 1 cut(s) 739
BsiHKCI CYCGRG 2 cut(s) 420, 680
BslFI GGGAC 1 cut(s) 235
BslI CCNNNNNNNGG 6 cut(s) 134, 265, 595, 713, 746, 809
BsmAI GTCTC 2 cut(s) 279, 767
BsmFI GGGAC 1 cut(s) 235
BsnI GGCC 1 cut(s) 739
BsoBI CYCGRG 2 cut(s) 420, 680
Bsp143I GATC 5 cut(s) 86, 297, 446, 703, 716
BspANI GGCC 1 cut(s) 739
BspCNI CTCAG 1 cut(s) 733
BspLI GGNNCC 4 cut(s) 479, 705, 750, 801
BspMI ACCTGC 1 cut(s) 108
BspPI GGATC 4 cut(s) 454, 698, 711, 724
BsrDI GCAATG 1 cut(s) 401
BsrI ACTGG 2 cut(s) 599, 803
BssECI CCNNGG 1 cut(s) 626
BssMI GATC 5 cut(s) 86, 297, 446, 703, 716
BssT1I CCWWGG 1 cut(s) 626
Bst6I CTCTTC 5 cut(s) 67, 96, 190, 501, 818
BstDEI CTNAG 1 cut(s) 741
BstF5I GGATG 1 cut(s) 544
BstHHI GCGC 1 cut(s) 364
BstKTI GATC 5 cut(s) 89, 300, 449, 706, 719
BstMAI GTCTC 2 cut(s) 279, 767
BstMBI GATC 5 cut(s) 86, 297, 446, 703, 716
BstMWI GCNNNNNNNGC 1 cut(s) 361
BstNSI RCATGY 1 cut(s) 392
BstSFI CTRYAG 1 cut(s) 394
BstX2I RGATCY 3 cut(s) 86, 446, 703
BstYI RGATCY 3 cut(s) 86, 446, 703
BsuI GTATCC 1 cut(s) 131
BsuRI GGCC 1 cut(s) 739
BtsCI GGATG 1 cut(s) 544
BveI ACCTGC 1 cut(s) 108
CfoI GCGC 1 cut(s) 364
Cfr13I GGNCC 2 cut(s) 737, 749
Csp6I GTAC 2 cut(s) 256, 399
CviAII CATG 2 cut(s) 5, 389
CviJI RGCY 9 cut(s) 17, 43, 355, 480, 494, 625, 739, 802, 814
CviKI_1 RGCY 9 cut(s) 17, 43, 355, 480, 494, 625, 739, 802, 814
CviQI GTAC 2 cut(s) 256, 399
DdeI CTNAG 1 cut(s) 741
DpnI GATC 5 cut(s) 88, 299, 448, 705, 718
DpnII GATC 5 cut(s) 86, 297, 446, 703, 716
DraI TTTAAA 1 cut(s) 568
Eam1104I CTCTTC 5 cut(s) 67, 96, 190, 501, 818
EarI CTCTTC 5 cut(s) 67, 96, 190, 501, 818
Eco130I CCWWGG 1 cut(s) 626
Eco47I GGWCC 1 cut(s) 749
Eco88I CYCGRG 2 cut(s) 420, 680
EcoO109I RGGNCCY 1 cut(s) 737
EcoRI GAATTC 2 cut(s) 409, 584
EcoT14I CCWWGG 1 cut(s) 626
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 1 cut(s) 626
FaeI CATG 2 cut(s) 8, 392
FaiI YATR 9 cut(s) 6, 377, 390, 396, 432, 444, 516, 698, 808
FaqI GGGAC 1 cut(s) 235
FatI CATG 2 cut(s) 4, 388
FokI GGATG 1 cut(s) 551
FspBI CTAG 1 cut(s) 815
FspI TGCGCA 1 cut(s) 363
GlaI GCGC 1 cut(s) 363
HaeIII GGCC 1 cut(s) 739
HhaI GCGC 1 cut(s) 364
Hin1II CATG 2 cut(s) 8, 392
Hin6I GCGC 1 cut(s) 362
HinP1I GCGC 1 cut(s) 362
HinfI GANTC 6 cut(s) 160, 238, 248, 434, 519, 631
HphI GGTGA 1 cut(s) 326
Hpy188I TCNGA 6 cut(s) 110, 315, 451, 652, 721, 758
Hpy188III TCNNGA 9 cut(s) 84, 196, 295, 301, 497, 589, 680, 682, 707
Hpy99I CGWCG 3 cut(s) 170, 219, 222
HpyAV CCTTC 5 cut(s) 288, 298, 484, 728, 739
HpyCH4IV ACGT 1 cut(s) 220
HpyCH4V TGCA 3 cut(s) 4, 103, 788
HpyF10VI GCNNNNNNNGC 1 cut(s) 361
HpyF3I CTNAG 1 cut(s) 741
HpySE526I ACGT 1 cut(s) 220
Hsp92II CATG 2 cut(s) 8, 392
HspAI GCGC 1 cut(s) 362
Kzo9I GATC 5 cut(s) 86, 297, 446, 703, 716
LmnI GCTCC 2 cut(s) 477, 799
MaeI CTAG 1 cut(s) 815
MaeII ACGT 1 cut(s) 220
MaeIII GTNAC 1 cut(s) 226
MalI GATC 5 cut(s) 88, 299, 448, 705, 718
MboI GATC 5 cut(s) 86, 297, 446, 703, 716
MflI RGATCY 3 cut(s) 86, 446, 703
MlyI GAGTC 4 cut(s) 247, 257, 428, 640
MmeI TCCRAC 2 cut(s) 630, 694
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 3 cut(s) 345, 384, 567
MslI CAYNNNNRTG 1 cut(s) 339
MwoI GCNNNNNNNGC 1 cut(s) 361
NdeII GATC 5 cut(s) 86, 297, 446, 703, 716
NlaIII CATG 2 cut(s) 8, 392
NlaIV GGNNCC 4 cut(s) 479, 705, 750, 801
NsbI TGCGCA 1 cut(s) 363
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 392
PaeR7I CTCGAG 1 cut(s) 680
PcsI WCGNNNNNNNCGW 1 cut(s) 194
PfeI GAWTC 2 cut(s) 160, 519
PflMI CCANNNNNTGG 2 cut(s) 265, 713
PleI GAGTC 4 cut(s) 246, 256, 428, 639
PpsI GAGTC 4 cut(s) 246, 256, 428, 639
PspN4I GGNNCC 4 cut(s) 479, 705, 750, 801
PspPI GGNCC 2 cut(s) 737, 749
PsuI RGATCY 3 cut(s) 86, 446, 703
RsaI GTAC 2 cut(s) 257, 400
RsaNI GTAC 2 cut(s) 256, 399
RseI CAYNNNNRTG 1 cut(s) 339
SaqAI TTAA 3 cut(s) 345, 384, 567
Sau3AI GATC 5 cut(s) 86, 297, 446, 703, 716
Sau96I GGNCC 2 cut(s) 737, 749
SchI GAGTC 4 cut(s) 247, 257, 428, 640
SetI ASST 6 cut(s) 45, 60, 102, 159, 223, 714
SfcI CTRYAG 1 cut(s) 394
Sfr274I CTCGAG 1 cut(s) 680
SinI GGWCC 1 cut(s) 749
SlaI CTCGAG 1 cut(s) 680
SmiMI CAYNNNNRTG 1 cut(s) 339
SmlI CTYRAG 3 cut(s) 326, 495, 680
SmoI CTYRAG 3 cut(s) 326, 495, 680
SspI AATATT 1 cut(s) 831
SspMI CTAG 1 cut(s) 815
StyI CCWWGG 1 cut(s) 626
TaiI ACGT 1 cut(s) 223
TaqI TCGA 4 cut(s) 188, 197, 294, 681
TatI WGTACW 1 cut(s) 398
TfiI GAWTC 2 cut(s) 160, 519
Tru1I TTAA 3 cut(s) 345, 384, 567
Tru9I TTAA 3 cut(s) 345, 384, 567
TspDTI ATGAA 2 cut(s) 516, 747
TspGWI ACGGA 2 cut(s) 716, 794
Van91I CCANNNNNTGG 2 cut(s) 265, 713
VpaK11BI GGWCC 1 cut(s) 749
XapI RAATTY 7 cut(s) 199, 371, 409, 584, 665, 674, 760
XceI RCATGY 1 cut(s) 392
XcmI CCANNNNNNNNNTGG 1 cut(s) 633
XhoI CTCGAG 1 cut(s) 680
XspI CTAG 1 cut(s) 815
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.