Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0001856

Physical Location & Seq

Genomic Coordinates Forward (+)

8328325 .. 8332527

Gene Structure 4203 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1096800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1041 bp

ATGGCAAACAATTCTACCAATGGGGAGCACCAGACCACAACAAAGCCTCCTCCTTTGCCATCTCCTCTGCGGTTCTCCAAGTTTTTTCAGTCCAATATGAGAATTTTGGTTACTGGAGGAGCTGGATTCATCGGTTCTCACCTGGTTGACAGGTTAATGGAAAATGAAAAGAATGAGGTTATTGTCGTTGATAACTACTTCACTGGCTCCAAGGACAATCTGAAAAAGTGGATTGGTCATCCCAGATTCGAGCTTATTCGTCATGATGTCACAGAGACGCTGTTGGTCGAGGTTGATCAGATTTACCATCTTGCATGCCCAGCTTCTCCAATTTTTTACAAATACAATCCTGTAAAGACAATAAAAACAAATGTGATTGGCACGCTGAACATGCTTGGACTTGCAAAGCGAGTTGGAGCAAGGATTCTGCTGACATCCACTTCAGAGGTATATGGTGATCCTCTTGTGCATCCACAACCTGAAAGCTACTGGGGTAATGTTAACCCAATTGGAGTAAGGAGCTGTTATGATGAGGGGAAGCGTGTTGCTGAGACTTTGATGTTCGACTATCATAGGCAGCATGGGATAGAAATACGTATTGCGAGAATCTTCAACACATATGGCCCCCGCATGAATATTGATGATGGACGTGTTGTGAGCAATTTTATAGCTCAGGCACTTCGTGATGAACCGTTGACAGTTCAGAATCCTGGGACTCAAACTCGCAGTTTCTGTTATGTCTCCGATCTGGTTGATGGCCTCATTCGTCTCATGGAAGGAGAGCACACCGGACCTATTAACCTCGGAAACCCAGGTGAATTTACCATGCTTGAACTTGCAGAGACGGTAAAGGAGCTCATCAACCCCGAGGTGGAGATCAAGAGGGTGGAGAACACTCCCGACGATCCAAGACAGAGGAAACCAGACATCACAAAGGCAAAAGAATTGCTGGGATGGGAGCCGAAGATCAAGTTGCGCGAAGGCCTACCCCTCATGGAGGAGGATTTCCGATTGAGGCTTGGGGTAAACGAAAAGAAATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005886 GO:0005975 GO:0005996 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016043 GO:0016829 GO:0016830 GO:0016831 GO:0019321 GO:0022607 GO:0036094 GO:0042732 GO:0042802 GO:0042803 GO:0043167 GO:0043168 GO:0043933 GO:0044085 GO:0044238 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046983 GO:0048037 GO:0048040 GO:0050662 GO:0051259 GO:0051262 GO:0051287 GO:0065003 GO:0070403 GO:0071704 GO:0071840 GO:0071944 GO:0097159 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00520 ko01100 map00520 map01100

Pfam Domains

Protein Families

GDP_Man_Dehyd

Protein Analysis

347

Amino Acids

39.26

Weight (kDa)

6.53

Isoelectric Point (pI)

29.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RmlD_sub_bind	PF04321	33 - 291	9.3e-09	RmlD substrate binding domain
Epimerase	PF01370	35 - 269	1.7e-47	NAD dependent epimerase/dehydratase family
Polysacc_synt_2	PF02719	35 - 143	1.8e-08	Polysaccharide biosynthesis protein
GDP_Man_Dehyd	PF16363	36 - 330	8.1e-51	GDP-mannose 4,6 dehydratase
3Beta_HSD	PF01073	36 - 187	3e-12	3-beta hydroxysteroid dehydrogenase/isomerase family
NAD_binding_4	PF07993	89 - 205	7.4e-07	Male sterility protein

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0001856)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g35550 FvH4_3g35550 FvH4_3g35550 FvH4_3g35550 FvH4_3g35550 FvH4_3g35550 FvH4_3g35550 FvH4_3g35550 FvH4_3g35550 FvH4_3g35550 FvH4_3g35550 FvH4_3g35550
malus_domestica	MD03G1096800.v1.1 MD11G1143300.v1.1
prunus_persica	Prupe.6G081100_v2.0.a1 Prupe.6G081100_v2.0.a1 Prupe.6G081100_v2.0.a1 Prupe.6G081100_v2.0.a1 Prupe.6G081100_v2.0.a1 Prupe.6G081100_v2.0.a1
pyrus_communis	pycom03g08000 pycom11g09310
rosa_chinensis	RchiOBHm_Chr5g0063761
rosa_laevigata	RLG00000035666
rosa_multiflora	Rmu_sc0002724.1_g000023
rosa_roxburghii	Rroxscaffold_1G00017090 Rroxscaffold_1G00037850 Rroxscaffold_3G00246740
rosa_rugosa	Rorug03G0248000 Rorug05G0359100 Rorug05G0359200 Rorug05G0359200 Rorug05G0359200 Rorug05G0359200 Rorug05G0359300 Rorug05G0359400
rosa_samantha	Rh2BG210700 Rh5AG128600 Rh5AG389400 Rh5BG433300 Rh5CG456500 Rh5DG446900 Rh5DG449700 Rh6AG145900
rosa_wichuraiana	Rw4G004490 Rw5G039270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	1 cut(s)	978
AciI	CCGC	2 cut(s)	70, 628
AclWI	GGATC	2 cut(s)	452, 899
AcsI	RAATTY	2 cut(s)	102, 818
AcuI	CTGAAG	1 cut(s)	426
AdeI	CACNNNGTG	1 cut(s)	683
AfiI	CCNNNNNNNGG	2 cut(s)	871, 997
AflIII	ACRYGT	1 cut(s)	649
AgsI	TTSAA	2 cut(s)	613, 833
AjiI	CACGTC	1 cut(s)	650
AjnI	CCWGG	3 cut(s)	141, 709, 811
AluBI	AGCT	7 cut(s)	122, 253, 323, 486, 522, 671, 856
AluI	AGCT	7 cut(s)	122, 253, 323, 486, 522, 671, 856
Alw21I	GWGCWC	3 cut(s)	30, 786, 858
Alw26I	GTCTC	5 cut(s)	269, 545, 745, 773, 836
AlwI	GGATC	2 cut(s)	452, 899
AlwNI	CAGNNNCTG	1 cut(s)	732
Ama87I	CYCGRG	1 cut(s)	866
AoxI	GGCC	3 cut(s)	622, 757, 982
ApeKI	GCWGC	1 cut(s)	577
ApoI	RAATTY	2 cut(s)	102, 818
AspLEI	GCGC	1 cut(s)	978
AspS9I	GGNCC	2 cut(s)	623, 791
AsuHPI	GGTGA	3 cut(s)	131, 467, 827
AvaI	CYCGRG	1 cut(s)	866
AvaII	GGWCC	1 cut(s)	791
BanII	GRGCYC	1 cut(s)	858
Bbv12I	GWGCWC	3 cut(s)	30, 786, 858
BbvI	GCAGC	1 cut(s)	589
BccI	CCATC	5 cut(s)	67, 315, 638, 749, 948
BciT130I	CCWGG	3 cut(s)	143, 711, 813
BclI	TGATCA	1 cut(s)	295
BcoDI	GTCTC	5 cut(s)	269, 545, 745, 773, 836
BisI	GCNGC	1 cut(s)	578
BlsI	GCNGC	1 cut(s)	579
Bme1390I	CCNGG	3 cut(s)	143, 711, 813
Bme18I	GGWCC	1 cut(s)	791
BmeT110I	CYCGRG	1 cut(s)	866
BmgBI	CACGTC	1 cut(s)	650
BmgT120I	GGNCC	2 cut(s)	623, 791
BmiI	GGNNCC	3 cut(s)	208, 625, 960
BmrFI	CCNGG	3 cut(s)	143, 711, 813
BmrI	ACTGGG	1 cut(s)	499
BmsI	GCATC	1 cut(s)	478
BmuI	ACTGGG	1 cut(s)	499
BpmI	CTGGAG	1 cut(s)	135
Bpu10I	CCTNAGC	1 cut(s)	672
BsaAI	YACGTR	1 cut(s)	596
BsaJI	CCNNGG	5 cut(s)	210, 710, 802, 811, 867
BsaWI	WCCGGW	1 cut(s)	788
BsaXI	ACNNNNNCTCC	2 cut(s)	31, 61
Bsc4I	CCNNNNNNNGG	2 cut(s)	871, 997
Bse1I	ACTGG	3 cut(s)	118, 208, 494
BseBI	CCWGG	3 cut(s)	143, 711, 813
BseDI	CCNNGG	5 cut(s)	210, 710, 802, 811, 867
BseGI	GGATG	4 cut(s)	238, 434, 469, 959
BseLI	CCNNNNNNNGG	2 cut(s)	871, 997
BseMII	CTCAG	2 cut(s)	540, 686
BseNI	ACTGG	3 cut(s)	118, 208, 494
BseRI	GAGGAG	4 cut(s)	39, 54, 132, 1013
BseXI	GCAGC	1 cut(s)	589
BseYI	CCCAGC	2 cut(s)	319, 949
Bsh1236I	CGCG	1 cut(s)	978
BshFI	GGCC	3 cut(s)	624, 759, 984
BsiHKAI	GWGCWC	3 cut(s)	30, 786, 858
BsiHKCI	CYCGRG	1 cut(s)	866
BsiSI	CCGG	1 cut(s)	789
BslFI	GGGAC	1 cut(s)	727
BslI	CCNNNNNNNGG	2 cut(s)	871, 997
BsmAI	GTCTC	5 cut(s)	269, 545, 745, 773, 836
BsmBI	CGTCTC	3 cut(s)	269, 773, 836
BsmFI	GGGAC	1 cut(s)	727
BsnI	GGCC	3 cut(s)	624, 759, 984
BsoBI	CYCGRG	1 cut(s)	866
Bsp1286I	GDGCHC	3 cut(s)	30, 786, 858
Bsp143I	GATC	6 cut(s)	295, 457, 745, 876, 904, 966
BspACI	CCGC	2 cut(s)	70, 628
BspANI	GGCC	3 cut(s)	624, 759, 984
BspCNI	CTCAG	2 cut(s)	541, 685
BspFNI	CGCG	1 cut(s)	978
BspHI	TCATGA	1 cut(s)	262
BspLI	GGNNCC	3 cut(s)	208, 625, 960
BspPI	GGATC	2 cut(s)	452, 899
BsrI	ACTGG	3 cut(s)	118, 208, 494
BssECI	CCNNGG	5 cut(s)	210, 710, 802, 811, 867
BssMI	GATC	6 cut(s)	295, 457, 745, 876, 904, 966
BssT1I	CCWWGG	1 cut(s)	210
Bst2UI	CCWGG	3 cut(s)	143, 711, 813
Bst4CI	ACNGT	3 cut(s)	693, 700, 847
BstBAI	YACGTR	1 cut(s)	596
BstC8I	GCNNGC	2 cut(s)	316, 383
BstDEI	CTNAG	2 cut(s)	549, 672
BstENI	CCTNNNNNAGG	1 cut(s)	995
BstF5I	GGATG	4 cut(s)	238, 434, 469, 959
BstFNI	CGCG	1 cut(s)	978
BstHHI	GCGC	1 cut(s)	978
BstKTI	GATC	6 cut(s)	298, 460, 748, 879, 907, 969
BstMAI	GTCTC	5 cut(s)	269, 545, 745, 773, 836
BstMBI	GATC	6 cut(s)	295, 457, 745, 876, 904, 966
BstMWI	GCNNNNNNNGC	2 cut(s)	320, 391
BstNI	CCWGG	3 cut(s)	143, 711, 813
BstNSI	RCATGY	2 cut(s)	318, 394
BstSCI	CCNGG	3 cut(s)	141, 709, 811
BstSNI	TACGTA	1 cut(s)	596
BstUI	CGCG	1 cut(s)	978
BstV1I	GCAGC	1 cut(s)	589
BsuRI	GGCC	3 cut(s)	624, 759, 984
BtrI	CACGTC	1 cut(s)	650
BtsCI	GGATG	4 cut(s)	238, 434, 469, 959
BtsIMutI	CAGTG	1 cut(s)	201
Cac8I	GCNNGC	2 cut(s)	316, 383
CaiI	CAGNNNCTG	1 cut(s)	732
CciI	TCATGA	1 cut(s)	262
CfoI	GCGC	1 cut(s)	978
Cfr13I	GGNCC	2 cut(s)	623, 791
CseI	GACGC	1 cut(s)	286
CsiI	ACCWGGT	1 cut(s)	141
CviAII	CATG	8 cut(s)	263, 315, 391, 581, 631, 772, 826, 994
DdeI	CTNAG	2 cut(s)	549, 672
DpnI	GATC	6 cut(s)	297, 459, 747, 878, 906, 968
DpnII	GATC	6 cut(s)	295, 457, 745, 876, 904, 966
DraIII	CACNNNGTG	1 cut(s)	683
Ecl136II	GAGCTC	1 cut(s)	856
Eco105I	TACGTA	1 cut(s)	596
Eco130I	CCWWGG	1 cut(s)	210
Eco147I	AGGCCT	1 cut(s)	984
Eco24I	GRGCYC	1 cut(s)	858
Eco47I	GGWCC	1 cut(s)	791
Eco53kI	GAGCTC	1 cut(s)	856
Eco57I	CTGAAG	1 cut(s)	426
Eco88I	CYCGRG	1 cut(s)	866
EcoICRI	GAGCTC	1 cut(s)	856
EcoNI	CCTNNNNNAGG	1 cut(s)	995
EcoRII	CCWGG	3 cut(s)	141, 709, 811
EcoT14I	CCWWGG	1 cut(s)	210
EcoT38I	GRGCYC	1 cut(s)	858
ErhI	CCWWGG	1 cut(s)	210
Esp3I	CGTCTC	3 cut(s)	269, 773, 836
FaeI	CATG	8 cut(s)	266, 318, 394, 584, 634, 775, 829, 997
FaqI	GGGAC	1 cut(s)	727
FatI	CATG	8 cut(s)	262, 314, 390, 580, 630, 771, 825, 993
FauI	CCCGC	1 cut(s)	635
FauNDI	CATATG	1 cut(s)	619
FbaI	TGATCA	1 cut(s)	295
Fnu4HI	GCNGC	1 cut(s)	578
FokI	GGATG	4 cut(s)	225, 421, 456, 966
FriOI	GRGCYC	1 cut(s)	858
Fsp4HI	GCNGC	1 cut(s)	578
GlaI	GCGC	1 cut(s)	977
GluI	GCNGC	1 cut(s)	578
GsaI	CCCAGC	2 cut(s)	323, 953
GsuI	CTGGAG	1 cut(s)	135
HaeIII	GGCC	3 cut(s)	624, 759, 984
HapII	CCGG	1 cut(s)	789
HgaI	GACGC	1 cut(s)	286
HhaI	GCGC	1 cut(s)	978
Hin1II	CATG	8 cut(s)	266, 318, 394, 584, 634, 775, 829, 997
Hin6I	GCGC	1 cut(s)	976
HinP1I	GCGC	1 cut(s)	976
HincII	GTYRAC	3 cut(s)	148, 502, 696
HindII	GTYRAC	3 cut(s)	148, 502, 696
HinfI	GANTC	6 cut(s)	126, 246, 424, 606, 706, 715
HpaI	GTTAAC	1 cut(s)	502
HpaII	CCGG	1 cut(s)	789
HphI	GGTGA	3 cut(s)	131, 467, 827
Hpy166II	GTNNAC	4 cut(s)	148, 502, 696, 1027
Hpy188I	TCNGA	7 cut(s)	222, 300, 445, 705, 745, 806, 1010
Hpy188III	TCNNGA	4 cut(s)	263, 683, 880, 899
Hpy8I	GTNNAC	4 cut(s)	148, 502, 696, 1027
Hpy99I	CGWCG	1 cut(s)	905
HpyAV	CCTTC	2 cut(s)	770, 974
HpyCH4III	ACNGT	3 cut(s)	693, 700, 847
HpyCH4IV	ACGT	2 cut(s)	595, 649
HpyCH4V	TGCA	4 cut(s)	314, 404, 469, 839
HpyF10VI	GCNNNNNNNGC	2 cut(s)	320, 391
HpyF3I	CTNAG	2 cut(s)	549, 672
HpySE526I	ACGT	2 cut(s)	595, 649
Hsp92II	CATG	8 cut(s)	266, 318, 394, 584, 634, 775, 829, 997
HspAI	GCGC	1 cut(s)	976
Ksp22I	TGATCA	1 cut(s)	295
KspAI	GTTAAC	1 cut(s)	502
Kzo9I	GATC	6 cut(s)	295, 457, 745, 876, 904, 966
LmnI	GCTCC	7 cut(s)	25, 119, 212, 416, 519, 853, 958
Lsp1109I	GCAGC	1 cut(s)	589
LweI	GCATC	1 cut(s)	478
MabI	ACCWGGT	1 cut(s)	141
MaeII	ACGT	2 cut(s)	595, 649
MaeIII	GTNAC	2 cut(s)	109, 268
MalI	GATC	6 cut(s)	297, 459, 747, 878, 906, 968
MboI	GATC	6 cut(s)	295, 457, 745, 876, 904, 966
MboII	GAAGA	2 cut(s)	601, 976
MfeI	CAATTG	1 cut(s)	507
MhlI	GDGCHC	3 cut(s)	30, 786, 858
MluCI	AATT	7 cut(s)	10, 102, 330, 507, 661, 818, 944
MlyI	GAGTC	1 cut(s)	709
MmeI	TCCRAC	1 cut(s)	394
MseI	TTAA	3 cut(s)	155, 501, 798
MspI	CCGG	1 cut(s)	789
MspR9I	CCNGG	3 cut(s)	143, 711, 813
MunI	CAATTG	1 cut(s)	507
MvaI	CCWGG	3 cut(s)	143, 711, 813
MvnI	CGCG	1 cut(s)	978
MwoI	GCNNNNNNNGC	2 cut(s)	320, 391
NdeI	CATATG	1 cut(s)	619
NdeII	GATC	6 cut(s)	295, 457, 745, 876, 904, 966
NlaIII	CATG	8 cut(s)	266, 318, 394, 584, 634, 775, 829, 997
NlaIV	GGNNCC	3 cut(s)	208, 625, 960
NmuCI	GTSAC	1 cut(s)	268
NspI	RCATGY	2 cut(s)	318, 394
PaeI	GCATGC	1 cut(s)	318
PagI	TCATGA	1 cut(s)	262
PceI	AGGCCT	1 cut(s)	984
PfeI	GAWTC	5 cut(s)	126, 246, 424, 606, 706
PkrI	GCNGC	1 cut(s)	579
PleI	GAGTC	1 cut(s)	709
PpsI	GAGTC	1 cut(s)	709
Ppu21I	YACGTR	1 cut(s)	596
Psp124BI	GAGCTC	1 cut(s)	858
Psp6I	CCWGG	3 cut(s)	141, 709, 811
PspFI	CCCAGC	2 cut(s)	319, 949
PspGI	CCWGG	3 cut(s)	141, 709, 811
PspN4I	GGNNCC	3 cut(s)	208, 625, 960
PspPI	GGNCC	2 cut(s)	623, 791
PstNI	CAGNNNCTG	1 cut(s)	732
SacI	GAGCTC	1 cut(s)	858
SaqAI	TTAA	3 cut(s)	155, 501, 798
SatI	GCNGC	1 cut(s)	578
Sau3AI	GATC	6 cut(s)	295, 457, 745, 876, 904, 966
Sau96I	GGNCC	2 cut(s)	623, 791
SchI	GAGTC	1 cut(s)	709
ScrFI	CCNGG	3 cut(s)	143, 711, 813
SduI	GDGCHC	3 cut(s)	30, 786, 858
SexAI	ACCWGGT	1 cut(s)	141
SfaNI	GCATC	1 cut(s)	478
SinI	GGWCC	1 cut(s)	791
SnaBI	TACGTA	1 cut(s)	596
SphI	GCATGC	1 cut(s)	318
Sse9I	AATT	7 cut(s)	10, 102, 330, 507, 661, 818, 944
SseBI	AGGCCT	1 cut(s)	984
SsiI	CCGC	2 cut(s)	70, 628
SspI	AATATT	1 cut(s)	637
SstI	GAGCTC	1 cut(s)	858
StuI	AGGCCT	1 cut(s)	984
StyD4I	CCNGG	3 cut(s)	141, 709, 811
StyI	CCWWGG	1 cut(s)	210
TaaI	ACNGT	3 cut(s)	693, 700, 847
TaiI	ACGT	2 cut(s)	598, 652
TaqI	TCGA	3 cut(s)	249, 288, 564
TasI	AATT	7 cut(s)	10, 102, 330, 507, 661, 818, 944
TfiI	GAWTC	5 cut(s)	126, 246, 424, 606, 706
Tru1I	TTAA	3 cut(s)	155, 501, 798
Tru9I	TTAA	3 cut(s)	155, 501, 798
TscAI	CASTG	1 cut(s)	208
TseFI	GTSAC	1 cut(s)	268
TseI	GCWGC	1 cut(s)	577
Tsp45I	GTSAC	1 cut(s)	268
TspDTI	ATGAA	4 cut(s)	118, 180, 647, 702
TspRI	CASTG	1 cut(s)	208
VpaK11BI	GGWCC	1 cut(s)	791
XagI	CCTNNNNNAGG	1 cut(s)	995
XapI	RAATTY	2 cut(s)	102, 818
XceI	RCATGY	2 cut(s)	318, 394

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.